Rw6G024230

protein At4g13200, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0012498
Physical Location & Seq
Reverse (-)
47102230 .. 47104430
2201 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G024230.1

Sequence Viewer

Length: 642 bp
ATGATTAGTGGGGCTTTAGCTTCAGCTTCCCCTCAAACTTTTTCTTCTTCATCATCTCTCGTATCTAAACCCAAAACCAGCTGTTACAGCTATGCTTCCACCTCTGTCAGTGTTGCCAGGCTAAGCTTCTTGGATCCGAAGCTTGAAAGCTTTGGACTTTATGGAGTAAAGGGGTCGAGGAGAGGCAGCAGAGTTCACTGCACTGCCGGACCTGGTGAGGGTGATAGCAAGAGTGTTCTGGATGCCTTTTTCTTGGGGAGAGCTCTAGCGGAAGCGGTAAATGAGCGAATTGAGTCCAGTGTCGGCGAGTTTTTGAGTACAATTGGTAGGCTGCAAGCTGAGCAACAAAAGCAAGTAGAGGAGTTTCAGGCAGACATACTGGACAGAGCCAAGAAAGCTAAAGAGAAAGCAGCACGTGAGGCTGCAGAGGCACGAGGAATTGTTTCAAAATCTACTACAGAAAGTGTAACACCAGTCACCGTCACCAATGGTGTTTCGCCAATAACTCCAACAACCACCACCACTGTTGACACTCCTGCTGTCAGTCAACCTTCATCGTCTGAGCCAACCCCCACAATAACTGAGAATGAACCGGGTCCTGCCAACGAGGATACATCTTCAGAGGTGCCAATTGATGATTGA

Protein Analysis

213

Amino Acids

22.29

Weight (kDa)

5.0

Isoelectric Point (pI)

46.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF6825 PF20711 76 - 174 1.8e-33 Family of unknown function (DUF6825)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012498)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13200
fragaria_vesca FvH4_2g19250
malus_domestica MD05G1177500.v1.1 MD10G1165900.v1.1
prunus_persica Prupe.8G204400_v2.0.a1
pyrus_communis pycom05g16340 pycom05g16390 pycom10g14310
rosa_chinensis RchiOBHm_Chr6g0285061
rosa_laevigata RLG00000012681
rosa_multiflora Rmu_sc0002478.1_g000008
rosa_roxburghii Rroxscaffold_7G00183770 Rroxscaffold_7G00184070
rosa_rugosa Rorug06G0170400
rosa_samantha Rh6AG282100 Rh6BG282600 Rh6CG284600 Rh6DG277800
rosa_wichuraiana Rw6G024230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 625
AciI CCGC 2 cut(s) 269, 275
AclWI GGATC 2 cut(s) 128, 141
AcuI CTGAAG 2 cut(s) 6, 603
AcvI CACGTG 1 cut(s) 416
AfaI GTAC 1 cut(s) 319
AfiI CCNNNNNNNGG 1 cut(s) 218
AgsI TTSAA 2 cut(s) 146, 447
AjnI CCWGG 2 cut(s) 116, 211
Alw21I GWGCWC 1 cut(s) 265
AlwI GGATC 2 cut(s) 128, 141
ApeKI GCWGC 4 cut(s) 186, 331, 410, 422
Asp700I GAANNNNTTC 1 cut(s) 442
AspS9I GGNCC 2 cut(s) 209, 596
AsuC2I CCSGG 1 cut(s) 594
AsuHPI GGTGA 4 cut(s) 227, 233, 469, 475
AvaII GGWCC 2 cut(s) 209, 596
BamHI GGATCC 1 cut(s) 133
BanI GGYRCC 1 cut(s) 625
BanII GRGCYC 1 cut(s) 265
BauI CACGAG 1 cut(s) 432
BbrPI CACGTG 1 cut(s) 416
Bbv12I GWGCWC 1 cut(s) 265
BbvI GCAGC 4 cut(s) 198, 318, 409, 422
BciT130I CCWGG 2 cut(s) 118, 213
BciVI GTATCC 1 cut(s) 604
BcnI CCSGG 1 cut(s) 594
BfaI CTAG 1 cut(s) 266
BfmI CTRYAG 2 cut(s) 423, 456
BfuI GTATCC 1 cut(s) 604
BisI GCNGC 4 cut(s) 187, 332, 411, 423
BlpI GCTNAGC 2 cut(s) 122, 339
BlsI GCNGC 4 cut(s) 188, 333, 412, 424
Bme1390I CCNGG 3 cut(s) 118, 213, 594
Bme18I GGWCC 2 cut(s) 209, 596
BmgT120I GGNCC 2 cut(s) 209, 596
BmiI GGNNCC 3 cut(s) 135, 597, 627
BmrFI CCNGG 3 cut(s) 118, 213, 594
BmsI GCATC 1 cut(s) 232
Bpu1102I GCTNAGC 2 cut(s) 122, 339
BpuMI CCSGG 1 cut(s) 594
BsaAI YACGTR 1 cut(s) 416
Bsc4I CCNNNNNNNGG 1 cut(s) 218
Bse1I ACTGG 3 cut(s) 297, 384, 473
BseBI CCWGG 2 cut(s) 118, 213
BseGI GGATG 1 cut(s) 247
BseLI CCNNNNNNNGG 1 cut(s) 218
BseMII CTCAG 3 cut(s) 330, 552, 573
BseNI ACTGG 3 cut(s) 297, 384, 473
BseRI GAGGAG 2 cut(s) 193, 374
BseXI GCAGC 4 cut(s) 198, 318, 409, 422
BsgI GTGCAG 1 cut(s) 184
BshNI GGYRCC 1 cut(s) 625
BsiHKAI GWGCWC 1 cut(s) 265
BsiSI CCGG 2 cut(s) 207, 593
BslI CCNNNNNNNGG 1 cut(s) 218
Bsp1286I GDGCHC 1 cut(s) 265
Bsp143I GATC 1 cut(s) 133
Bsp1720I GCTNAGC 2 cut(s) 122, 339
BspACI CCGC 2 cut(s) 269, 275
BspCNI CTCAG 3 cut(s) 331, 553, 574
BspLI GGNNCC 3 cut(s) 135, 597, 627
BspMAI CTGCAG 1 cut(s) 427
BspPI GGATC 2 cut(s) 128, 141
BspT107I GGYRCC 1 cut(s) 625
BsrI ACTGG 3 cut(s) 297, 384, 473
BssMI GATC 1 cut(s) 133
BssSI CACGAG 1 cut(s) 432
Bst2BI CACGAG 1 cut(s) 432
Bst2UI CCWGG 2 cut(s) 118, 213
Bst4CI ACNGT 2 cut(s) 481, 526
BstBAI YACGTR 1 cut(s) 416
BstC8I GCNNGC 1 cut(s) 336
BstDEI CTNAG 4 cut(s) 122, 339, 561, 582
BstF5I GGATG 1 cut(s) 247
BstKTI GATC 1 cut(s) 136
BstMBI GATC 1 cut(s) 133
BstMWI GCNNNNNNNGC 6 cut(s) 87, 340, 349, 395, 419, 428
BstNI CCWGG 2 cut(s) 118, 213
BstSCI CCNGG 3 cut(s) 116, 211, 592
BstSFI CTRYAG 2 cut(s) 423, 456
BstV1I GCAGC 4 cut(s) 198, 318, 409, 422
BstX2I RGATCY 1 cut(s) 133
BstYI RGATCY 1 cut(s) 133
BsuI GTATCC 1 cut(s) 604
BtsCI GGATG 1 cut(s) 247
BtsI GCAGTG 2 cut(s) 196, 201
BtsIMutI CAGTG 5 cut(s) 115, 196, 201, 304, 522
Cac8I GCNNGC 1 cut(s) 336
Cfr13I GGNCC 2 cut(s) 209, 596
CsiI ACCWGGT 1 cut(s) 211
Csp6I GTAC 1 cut(s) 318
CspCI CAANNNNNGTGG 2 cut(s) 508, 543
CviQI GTAC 1 cut(s) 318
DdeI CTNAG 4 cut(s) 122, 339, 561, 582
DpnI GATC 1 cut(s) 135
DpnII GATC 1 cut(s) 133
Ecl136II GAGCTC 1 cut(s) 263
Eco24I GRGCYC 1 cut(s) 265
Eco47I GGWCC 2 cut(s) 209, 596
Eco53kI GAGCTC 1 cut(s) 263
Eco57I CTGAAG 2 cut(s) 6, 603
Eco72I CACGTG 1 cut(s) 416
EcoICRI GAGCTC 1 cut(s) 263
EcoO109I RGGNCCY 1 cut(s) 596
EcoRII CCWGG 2 cut(s) 116, 211
EcoT38I GRGCYC 1 cut(s) 265
FaiI YATR 3 cut(s) 93, 162, 377
Fnu4HI GCNGC 4 cut(s) 187, 332, 411, 423
FokI GGATG 1 cut(s) 254
FriOI GRGCYC 1 cut(s) 265
Fsp4HI GCNGC 4 cut(s) 187, 332, 411, 423
FspBI CTAG 1 cut(s) 266
GluI GCNGC 4 cut(s) 187, 332, 411, 423
HapII CCGG 2 cut(s) 207, 593
HincII GTYRAC 2 cut(s) 529, 548
HindII GTYRAC 2 cut(s) 529, 548
HindIII AAGCTT 3 cut(s) 124, 140, 148
HinfI GANTC 1 cut(s) 293
HpaII CCGG 2 cut(s) 207, 593
HphI GGTGA 4 cut(s) 227, 233, 469, 475
Hpy166II GTNNAC 3 cut(s) 196, 529, 548
Hpy188I TCNGA 3 cut(s) 138, 562, 622
Hpy188III TCNNGA 1 cut(s) 239
Hpy8I GTNNAC 3 cut(s) 196, 529, 548
HpyAV CCTTC 1 cut(s) 561
HpyCH4III ACNGT 2 cut(s) 481, 526
HpyCH4IV ACGT 1 cut(s) 415
HpyCH4V TGCA 3 cut(s) 201, 334, 425
HpyF10VI GCNNNNNNNGC 6 cut(s) 87, 340, 349, 395, 419, 428
HpyF3I CTNAG 4 cut(s) 122, 339, 561, 582
HpySE526I ACGT 1 cut(s) 415
Kzo9I GATC 1 cut(s) 133
Lsp1109I GCAGC 4 cut(s) 198, 318, 409, 422
LweI GCATC 1 cut(s) 232
MabI ACCWGGT 1 cut(s) 211
MaeI CTAG 1 cut(s) 266
MaeII ACGT 1 cut(s) 415
MaeIII GTNAC 4 cut(s) 83, 466, 475, 481
MalI GATC 1 cut(s) 135
MboI GATC 1 cut(s) 133
MboII GAAGA 3 cut(s) 36, 39, 609
MfeI CAATTG 2 cut(s) 321, 630
MflI RGATCY 1 cut(s) 133
MhlI GDGCHC 1 cut(s) 265
MluCI AATT 4 cut(s) 288, 321, 438, 630
MlyI GAGTC 1 cut(s) 302
MmeI TCCRAC 1 cut(s) 533
MroXI GAANNNNTTC 1 cut(s) 442
MspA1I CMGCKG 1 cut(s) 81
MspI CCGG 2 cut(s) 207, 593
MspR9I CCNGG 3 cut(s) 118, 213, 594
MunI CAATTG 2 cut(s) 321, 630
MvaI CCWGG 2 cut(s) 118, 213
MwoI GCNNNNNNNGC 6 cut(s) 87, 340, 349, 395, 419, 428
NciI CCSGG 1 cut(s) 594
NdeII GATC 1 cut(s) 133
NlaIV GGNNCC 3 cut(s) 135, 597, 627
NmuCI GTSAC 2 cut(s) 475, 481
PdmI GAANNNNTTC 1 cut(s) 442
PkrI GCNGC 4 cut(s) 188, 333, 412, 424
PleI GAGTC 1 cut(s) 301
PmaCI CACGTG 1 cut(s) 416
PmlI CACGTG 1 cut(s) 416
PpsI GAGTC 1 cut(s) 301
Ppu21I YACGTR 1 cut(s) 416
PpuMI RGGWCCY 1 cut(s) 596
Psp124BI GAGCTC 1 cut(s) 265
Psp5II RGGWCCY 1 cut(s) 596
Psp6I CCWGG 2 cut(s) 116, 211
PspCI CACGTG 1 cut(s) 416
PspGI CCWGG 2 cut(s) 116, 211
PspN4I GGNNCC 3 cut(s) 135, 597, 627
PspPI GGNCC 2 cut(s) 209, 596
PspPPI RGGWCCY 1 cut(s) 596
PstI CTGCAG 1 cut(s) 427
PsuI RGATCY 1 cut(s) 133
PvuII CAGCTG 1 cut(s) 81
RsaI GTAC 1 cut(s) 319
RsaNI GTAC 1 cut(s) 318
SacI GAGCTC 1 cut(s) 265
SatI GCNGC 4 cut(s) 187, 332, 411, 423
Sau3AI GATC 1 cut(s) 133
Sau96I GGNCC 2 cut(s) 209, 596
SchI GAGTC 1 cut(s) 302
ScrFI CCNGG 3 cut(s) 118, 213, 594
SduI GDGCHC 1 cut(s) 265
SexAI ACCWGGT 1 cut(s) 211
SfaNI GCATC 1 cut(s) 232
SfcI CTRYAG 2 cut(s) 423, 456
SinI GGWCC 2 cut(s) 209, 596
Sse9I AATT 4 cut(s) 288, 321, 438, 630
SsiI CCGC 2 cut(s) 269, 275
SspMI CTAG 1 cut(s) 266
SstI GAGCTC 1 cut(s) 265
StyD4I CCNGG 3 cut(s) 116, 211, 592
TaaI ACNGT 2 cut(s) 481, 526
TaiI ACGT 1 cut(s) 418
TaqI TCGA 1 cut(s) 176
TasI AATT 4 cut(s) 288, 321, 438, 630
TatI WGTACW 1 cut(s) 317
TscAI CASTG 5 cut(s) 115, 203, 208, 304, 529
TseFI GTSAC 2 cut(s) 475, 481
TseI GCWGC 4 cut(s) 186, 331, 410, 422
Tsp45I GTSAC 2 cut(s) 475, 481
TspDTI ATGAA 3 cut(s) 39, 543, 603
TspRI CASTG 5 cut(s) 115, 203, 208, 304, 529
VpaK11BI GGWCC 2 cut(s) 209, 596
XmnI GAANNNNTTC 1 cut(s) 442
XspI CTAG 1 cut(s) 266
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.