MD10G1165900.v1.1

protein At4g13200, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0012498
Physical Location & Seq
Reverse (-)
25641339 .. 25642176
838 bp
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UTR
Exon/CDS
Intron
MD10G1165900.v1.1.491

Sequence Viewer

Length: 417 bp
ATGGTATGTGGGGTCTTAGCTTCTGCTTCTGCTCCCACACAAGCACTTTCTTCCCCGTTTTCTAAACCCAGAAGCACCAGCTTTTTCTCTGCATCGTCTTCAAGCTCTGGAAAGGTTCACACAGATTTGAAGCTCAAAACAGTCGGATTTTATGGAAGAAAAGGGTCTCAGAGAAGCATATTTCACTGCAGTAGCAGCGCAGGACCCGGTGGACCCGGATTTGGTGAGGGTGATAGCAGGAGTGTTCTGGATGCGTTTTTCTTGGGGAAAGCTCTAGCTGAAGCTATAAACGAGCGCATTGAGTCCGGTGTAGGCGAGTTTTTGAGCACGATTGGTAGGCTGCAAGCTGAGCAGCAGAAGCAAGTTGACGAGTTTCAGGTAAGAAGTTCCAGTTGCCGGGGTTCGGTTTACTCATGA

Protein Analysis

139

Amino Acids

14.45

Weight (kDa)

9.18

Isoelectric Point (pI)

49.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF6825 PF20711 79 - 127 2.2e-23 Family of unknown function (DUF6825)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012498)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13200
fragaria_vesca FvH4_2g19250
malus_domestica MD05G1177500.v1.1 MD10G1165900.v1.1
prunus_persica Prupe.8G204400_v2.0.a1
pyrus_communis pycom05g16340 pycom05g16390 pycom10g14310
rosa_chinensis RchiOBHm_Chr6g0285061
rosa_laevigata RLG00000012681
rosa_multiflora Rmu_sc0002478.1_g000008
rosa_roxburghii Rroxscaffold_7G00183770 Rroxscaffold_7G00184070
rosa_rugosa Rorug06G0170400
rosa_samantha Rh6AG282100 Rh6BG282600 Rh6CG284600 Rh6DG277800
rosa_wichuraiana Rw6G024230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcuI CTGAAG 1 cut(s) 300
AfiI CCNNNNNNNGG 3 cut(s) 221, 396, 403
AgsI TTSAA 2 cut(s) 102, 130
AluBI AGCT 8 cut(s) 20, 81, 105, 133, 272, 278, 284, 347
AluI AGCT 8 cut(s) 20, 81, 105, 133, 272, 278, 284, 347
Alw21I GWGCWC 1 cut(s) 329
Alw26I GTCTC 1 cut(s) 171
ApeKI GCWGC 3 cut(s) 195, 340, 352
AspLEI GCGC 2 cut(s) 200, 297
AspS9I GGNCC 2 cut(s) 203, 212
AsuC2I CCSGG 3 cut(s) 207, 216, 398
AsuHPI GGTGA 2 cut(s) 236, 242
AvaII GGWCC 2 cut(s) 203, 212
BbsI GAAGAC 1 cut(s) 90
Bbv12I GWGCWC 1 cut(s) 329
BbvI GCAGC 3 cut(s) 207, 327, 364
BcnI CCSGG 3 cut(s) 207, 216, 398
BcoDI GTCTC 1 cut(s) 171
BfaI CTAG 1 cut(s) 275
BfmI CTRYAG 1 cut(s) 187
BisI GCNGC 3 cut(s) 196, 341, 353
BlpI GCTNAGC 1 cut(s) 348
BlsI GCNGC 3 cut(s) 197, 342, 354
Bme1390I CCNGG 3 cut(s) 207, 216, 398
Bme18I GGWCC 2 cut(s) 203, 212
BmgT120I GGNCC 2 cut(s) 203, 212
BmiI GGNNCC 2 cut(s) 205, 214
BmrFI CCNGG 3 cut(s) 207, 216, 398
BmsI GCATC 2 cut(s) 101, 241
BpiI GAAGAC 1 cut(s) 90
Bpu1102I GCTNAGC 1 cut(s) 348
BpuMI CCSGG 3 cut(s) 207, 216, 398
BsaI GGTCTC 1 cut(s) 171
BsaJI CCNNGG 1 cut(s) 397
BsaWI WCCGGW 1 cut(s) 305
Bsc4I CCNNNNNNNGG 3 cut(s) 221, 396, 403
Bse1I ACTGG 1 cut(s) 390
BseDI CCNNGG 1 cut(s) 397
BseGI GGATG 1 cut(s) 256
BseLI CCNNNNNNNGG 3 cut(s) 221, 396, 403
BseMII CTCAG 2 cut(s) 182, 339
BseNI ACTGG 1 cut(s) 390
BseXI GCAGC 3 cut(s) 207, 327, 364
BsiHKAI GWGCWC 1 cut(s) 329
BsiSI CCGG 4 cut(s) 207, 216, 306, 397
BslI CCNNNNNNNGG 3 cut(s) 221, 396, 403
BsmAI GTCTC 1 cut(s) 171
Bso31I GGTCTC 1 cut(s) 171
Bsp1286I GDGCHC 1 cut(s) 329
Bsp1720I GCTNAGC 1 cut(s) 348
BspCNI CTCAG 2 cut(s) 181, 340
BspHI TCATGA 1 cut(s) 413
BspLI GGNNCC 2 cut(s) 205, 214
BspMAI CTGCAG 1 cut(s) 191
BspTNI GGTCTC 1 cut(s) 171
BsrI ACTGG 1 cut(s) 390
BssECI CCNNGG 1 cut(s) 397
Bst4CI ACNGT 1 cut(s) 142
BstC8I GCNNGC 1 cut(s) 345
BstDEI CTNAG 3 cut(s) 16, 168, 348
BstF5I GGATG 1 cut(s) 256
BstHHI GCGC 2 cut(s) 200, 297
BstMAI GTCTC 1 cut(s) 171
BstMWI GCNNNNNNNGC 3 cut(s) 195, 349, 358
BstSCI CCNGG 3 cut(s) 205, 214, 396
BstSFI CTRYAG 1 cut(s) 187
BstV1I GCAGC 3 cut(s) 207, 327, 364
BstV2I GAAGAC 1 cut(s) 90
BtsCI GGATG 1 cut(s) 256
BtsI GCAGTG 1 cut(s) 184
BtsIMutI CAGTG 1 cut(s) 184
Cac8I GCNNGC 1 cut(s) 345
CciI TCATGA 1 cut(s) 413
CfoI GCGC 2 cut(s) 200, 297
Cfr13I GGNCC 2 cut(s) 203, 212
CviAII CATG 1 cut(s) 414
CviJI RGCY 9 cut(s) 20, 81, 105, 133, 272, 278, 284, 340, 347
CviKI_1 RGCY 9 cut(s) 20, 81, 105, 133, 272, 278, 284, 340, 347
DdeI CTNAG 3 cut(s) 16, 168, 348
Eco31I GGTCTC 1 cut(s) 171
Eco47I GGWCC 2 cut(s) 203, 212
Eco57I CTGAAG 1 cut(s) 300
EcoO109I RGGNCCY 1 cut(s) 203
FaeI CATG 1 cut(s) 417
FaiI YATR 5 cut(s) 7, 153, 179, 287, 415
FatI CATG 1 cut(s) 413
Fnu4HI GCNGC 3 cut(s) 196, 341, 353
FokI GGATG 1 cut(s) 263
Fsp4HI GCNGC 3 cut(s) 196, 341, 353
FspBI CTAG 1 cut(s) 275
GlaI GCGC 2 cut(s) 199, 296
GluI GCNGC 3 cut(s) 196, 341, 353
HapII CCGG 4 cut(s) 207, 216, 306, 397
HhaI GCGC 2 cut(s) 200, 297
Hin1II CATG 1 cut(s) 417
Hin6I GCGC 2 cut(s) 198, 295
HinP1I GCGC 2 cut(s) 198, 295
HincII GTYRAC 1 cut(s) 367
HindII GTYRAC 1 cut(s) 367
HinfI GANTC 1 cut(s) 302
HpaII CCGG 4 cut(s) 207, 216, 306, 397
HphI GGTGA 2 cut(s) 236, 242
Hpy166II GTNNAC 4 cut(s) 118, 212, 367, 409
Hpy188I TCNGA 2 cut(s) 146, 171
Hpy188III TCNNGA 3 cut(s) 108, 248, 414
Hpy8I GTNNAC 4 cut(s) 118, 212, 367, 409
HpyCH4III ACNGT 1 cut(s) 142
HpyCH4V TGCA 3 cut(s) 92, 189, 343
HpyF10VI GCNNNNNNNGC 3 cut(s) 195, 349, 358
HpyF3I CTNAG 3 cut(s) 16, 168, 348
Hsp92II CATG 1 cut(s) 417
HspAI GCGC 2 cut(s) 198, 295
LmnI GCTCC 1 cut(s) 37
Lsp1109I GCAGC 3 cut(s) 207, 327, 364
LweI GCATC 2 cut(s) 101, 241
MaeI CTAG 1 cut(s) 275
MboII GAAGA 3 cut(s) 42, 90, 168
MhlI GDGCHC 1 cut(s) 329
MlyI GAGTC 1 cut(s) 311
MmeI TCCRAC 1 cut(s) 124
MnlI CCTC 1 cut(s) 220
MspI CCGG 4 cut(s) 207, 216, 306, 397
MspR9I CCNGG 3 cut(s) 207, 216, 398
MwoI GCNNNNNNNGC 3 cut(s) 195, 349, 358
NciI CCSGG 3 cut(s) 207, 216, 398
NlaIII CATG 1 cut(s) 417
NlaIV GGNNCC 2 cut(s) 205, 214
PagI TCATGA 1 cut(s) 413
PkrI GCNGC 3 cut(s) 197, 342, 354
PleI GAGTC 1 cut(s) 310
PpsI GAGTC 1 cut(s) 310
PpuMI RGGWCCY 1 cut(s) 203
Psp5II RGGWCCY 1 cut(s) 203
PspN4I GGNNCC 2 cut(s) 205, 214
PspPI GGNCC 2 cut(s) 203, 212
PspPPI RGGWCCY 1 cut(s) 203
PstI CTGCAG 1 cut(s) 191
SatI GCNGC 3 cut(s) 196, 341, 353
Sau96I GGNCC 2 cut(s) 203, 212
SchI GAGTC 1 cut(s) 311
ScrFI CCNGG 3 cut(s) 207, 216, 398
SduI GDGCHC 1 cut(s) 329
SfaNI GCATC 2 cut(s) 101, 241
SfcI CTRYAG 1 cut(s) 187
SinI GGWCC 2 cut(s) 203, 212
SspMI CTAG 1 cut(s) 275
StyD4I CCNGG 3 cut(s) 205, 214, 396
TaaI ACNGT 1 cut(s) 142
TscAI CASTG 1 cut(s) 191
TseI GCWGC 3 cut(s) 195, 340, 352
TspRI CASTG 1 cut(s) 191
VpaK11BI GGWCC 2 cut(s) 203, 212
XspI CTAG 1 cut(s) 275
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.