RLG00000012681

protein At4g13200, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0012498
Physical Location & Seq
Forward (+)
21259898 .. 21261638
1741 bp
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UTR
Exon/CDS
Intron
RLM00000012681

Sequence Viewer

Length: 642 bp
ATGATTAGTGGGGCTTTAGCTTCAGCTTCCCCACAAACTTTTTCTTCTTCATCATTTCTCGTATCTAAACCCAAAACCAGCTGTTACAGCTATGCTTCCACCTCCGTCAGTGTTGCCAAGCTAAGCTTCTTGGATCTGAAGCTTGAAAGCTCTGGACTTTATGGAGTGAAGGGGTCAAGGAGAGGCAGCAGAGTTCACTGCACTTCCGGACCTGGTGAGGGTGATAGCAAGAGTGTTCTGGATGCCTTCTTCTTGGGGAGAGCTCTAGCGGAAGCGCTAAATGAGCGAATTGAGTCCAGTGTCGGCGAGTTTTTGAGTACAATTGGTAGGCTGCAAGCTGAGCAACAAAAGCAAGTAGAGGACTTTCAGGCAGACGTACTGGACCGAGCCAAGAAAGCTAAAGAGAAAGCAGCACGTGAGGCTGCAGAGGCACGAGGAATTGTTTCAAAATCTACTACAGAAAGTGAAACACCAGTCAACGTCACCAATGGTGTTTCGCCAATACCTCCAACAACCACCACAACTGTTGACACTCCTGCTGTCAGTCAACCTTCATCGTCTGAGCCAACCCCCACAATAACTGAGAATGAACCAGGTCCTGCCGACGAGGATACATCTTCAGAGGTGCCAATTGATGATTGA

Protein Analysis

214

Amino Acids

22.32

Weight (kDa)

4.84

Isoelectric Point (pI)

45.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF6825 PF20711 76 - 172 3e-34 Family of unknown function (DUF6825)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012498)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13200
fragaria_vesca FvH4_2g19250
malus_domestica MD05G1177500.v1.1 MD10G1165900.v1.1
prunus_persica Prupe.8G204400_v2.0.a1
pyrus_communis pycom05g16340 pycom05g16390 pycom10g14310
rosa_chinensis RchiOBHm_Chr6g0285061
rosa_laevigata RLG00000012681
rosa_multiflora Rmu_sc0002478.1_g000008
rosa_roxburghii Rroxscaffold_7G00183770 Rroxscaffold_7G00184070
rosa_rugosa Rorug06G0170400
rosa_samantha Rh6AG282100 Rh6BG282600 Rh6CG284600 Rh6DG277800
rosa_wichuraiana Rw6G024230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 625
AccIII TCCGGA 1 cut(s) 206
AciI CCGC 1 cut(s) 269
AclWI GGATC 1 cut(s) 141
AcuI CTGAAG 3 cut(s) 6, 158, 603
AcvI CACGTG 1 cut(s) 416
AfaI GTAC 2 cut(s) 319, 378
AfeI AGCGCT 1 cut(s) 276
AfiI CCNNNNNNNGG 1 cut(s) 218
AgsI TTSAA 2 cut(s) 146, 447
AjnI CCWGG 2 cut(s) 211, 592
AjuI GAANNNNNNNTTGG 2 cut(s) 110, 142
Alw21I GWGCWC 1 cut(s) 265
AlwI GGATC 1 cut(s) 141
AlwNI CAGNNNCTG 1 cut(s) 599
Aor13HI TCCGGA 1 cut(s) 206
Aor51HI AGCGCT 1 cut(s) 276
ApeKI GCWGC 4 cut(s) 186, 331, 410, 422
Asp700I GAANNNNTTC 1 cut(s) 442
AspLEI GCGC 1 cut(s) 277
AspS9I GGNCC 3 cut(s) 209, 382, 596
AsuHPI GGTGA 3 cut(s) 227, 233, 475
AvaII GGWCC 3 cut(s) 209, 382, 596
BanI GGYRCC 1 cut(s) 625
BanII GRGCYC 1 cut(s) 265
BauI CACGAG 1 cut(s) 432
BbrPI CACGTG 1 cut(s) 416
Bbv12I GWGCWC 1 cut(s) 265
BbvI GCAGC 4 cut(s) 198, 318, 409, 422
BciT130I CCWGG 2 cut(s) 213, 594
BciVI GTATCC 1 cut(s) 604
BfaI CTAG 1 cut(s) 266
BfmI CTRYAG 2 cut(s) 423, 456
BfoI RGCGCY 1 cut(s) 278
BfuI GTATCC 1 cut(s) 604
BisI GCNGC 4 cut(s) 187, 332, 411, 423
BlpI GCTNAGC 2 cut(s) 122, 339
BlsI GCNGC 4 cut(s) 188, 333, 412, 424
Bme1390I CCNGG 2 cut(s) 213, 594
Bme18I GGWCC 3 cut(s) 209, 382, 596
BmgT120I GGNCC 3 cut(s) 209, 382, 596
BmiI GGNNCC 1 cut(s) 627
BmrFI CCNGG 2 cut(s) 213, 594
BmsI GCATC 1 cut(s) 232
Bpu1102I GCTNAGC 2 cut(s) 122, 339
BsaAI YACGTR 1 cut(s) 416
BsaWI WCCGGW 1 cut(s) 206
Bsc4I CCNNNNNNNGG 1 cut(s) 218
Bse1I ACTGG 3 cut(s) 297, 384, 473
BseAI TCCGGA 1 cut(s) 206
BseBI CCWGG 2 cut(s) 213, 594
BseGI GGATG 1 cut(s) 247
BseLI CCNNNNNNNGG 1 cut(s) 218
BseMII CTCAG 3 cut(s) 330, 552, 573
BseNI ACTGG 3 cut(s) 297, 384, 473
BseXI GCAGC 4 cut(s) 198, 318, 409, 422
BsgI GTGCAG 1 cut(s) 184
BshNI GGYRCC 1 cut(s) 625
BsiHKAI GWGCWC 1 cut(s) 265
BsiSI CCGG 1 cut(s) 207
BslI CCNNNNNNNGG 1 cut(s) 218
Bsp1286I GDGCHC 1 cut(s) 265
Bsp13I TCCGGA 1 cut(s) 206
Bsp143I GATC 1 cut(s) 133
Bsp1720I GCTNAGC 2 cut(s) 122, 339
BspACI CCGC 1 cut(s) 269
BspCNI CTCAG 3 cut(s) 331, 553, 574
BspEI TCCGGA 1 cut(s) 206
BspLI GGNNCC 1 cut(s) 627
BspMAI CTGCAG 1 cut(s) 427
BspPI GGATC 1 cut(s) 141
BspT107I GGYRCC 1 cut(s) 625
BsrI ACTGG 3 cut(s) 297, 384, 473
BssMI GATC 1 cut(s) 133
BssSI CACGAG 1 cut(s) 432
Bst2BI CACGAG 1 cut(s) 432
Bst2UI CCWGG 2 cut(s) 213, 594
Bst4CI ACNGT 1 cut(s) 526
BstBAI YACGTR 1 cut(s) 416
BstC8I GCNNGC 1 cut(s) 336
BstDEI CTNAG 4 cut(s) 122, 339, 561, 582
BstF5I GGATG 1 cut(s) 247
BstH2I RGCGCY 1 cut(s) 278
BstHHI GCGC 1 cut(s) 277
BstKTI GATC 1 cut(s) 136
BstMBI GATC 1 cut(s) 133
BstMWI GCNNNNNNNGC 7 cut(s) 87, 283, 340, 349, 395, 419, 428
BstNI CCWGG 2 cut(s) 213, 594
BstSCI CCNGG 2 cut(s) 211, 592
BstSFI CTRYAG 2 cut(s) 423, 456
BstV1I GCAGC 4 cut(s) 198, 318, 409, 422
BstX2I RGATCY 1 cut(s) 133
BstYI RGATCY 1 cut(s) 133
BsuI GTATCC 1 cut(s) 604
BtsCI GGATG 1 cut(s) 247
BtsI GCAGTG 1 cut(s) 196
BtsIMutI CAGTG 3 cut(s) 115, 196, 304
Cac8I GCNNGC 1 cut(s) 336
CaiI CAGNNNCTG 1 cut(s) 599
CfoI GCGC 1 cut(s) 277
Cfr13I GGNCC 3 cut(s) 209, 382, 596
CsiI ACCWGGT 2 cut(s) 211, 592
Csp6I GTAC 2 cut(s) 318, 377
CspCI CAANNNNNGTGG 2 cut(s) 508, 543
CviQI GTAC 2 cut(s) 318, 377
DdeI CTNAG 4 cut(s) 122, 339, 561, 582
DpnI GATC 1 cut(s) 135
DpnII GATC 1 cut(s) 133
Ecl136II GAGCTC 1 cut(s) 263
Eco24I GRGCYC 1 cut(s) 265
Eco47I GGWCC 3 cut(s) 209, 382, 596
Eco47III AGCGCT 1 cut(s) 276
Eco53kI GAGCTC 1 cut(s) 263
Eco57I CTGAAG 3 cut(s) 6, 158, 603
Eco72I CACGTG 1 cut(s) 416
EcoICRI GAGCTC 1 cut(s) 263
EcoO109I RGGNCCY 1 cut(s) 596
EcoRII CCWGG 2 cut(s) 211, 592
EcoT38I GRGCYC 1 cut(s) 265
FaiI YATR 2 cut(s) 93, 162
FalI AAGNNNNNCTT 2 cut(s) 110, 142
Fnu4HI GCNGC 4 cut(s) 187, 332, 411, 423
FokI GGATG 1 cut(s) 254
FriOI GRGCYC 1 cut(s) 265
Fsp4HI GCNGC 4 cut(s) 187, 332, 411, 423
FspBI CTAG 1 cut(s) 266
GlaI GCGC 1 cut(s) 276
GluI GCNGC 4 cut(s) 187, 332, 411, 423
HaeII RGCGCY 1 cut(s) 278
HapII CCGG 1 cut(s) 207
HhaI GCGC 1 cut(s) 277
Hin6I GCGC 1 cut(s) 275
HinP1I GCGC 1 cut(s) 275
HincII GTYRAC 3 cut(s) 478, 529, 548
HindII GTYRAC 3 cut(s) 478, 529, 548
HindIII AAGCTT 2 cut(s) 124, 140
HinfI GANTC 1 cut(s) 293
HpaII CCGG 1 cut(s) 207
HphI GGTGA 3 cut(s) 227, 233, 475
Hpy166II GTNNAC 4 cut(s) 196, 478, 529, 548
Hpy188I TCNGA 3 cut(s) 138, 562, 622
Hpy188III TCNNGA 3 cut(s) 153, 207, 239
Hpy8I GTNNAC 4 cut(s) 196, 478, 529, 548
Hpy99I CGWCG 1 cut(s) 608
HpyAV CCTTC 3 cut(s) 163, 256, 561
HpyCH4III ACNGT 1 cut(s) 526
HpyCH4IV ACGT 3 cut(s) 375, 415, 480
HpyCH4V TGCA 3 cut(s) 201, 334, 425
HpyF10VI GCNNNNNNNGC 7 cut(s) 87, 283, 340, 349, 395, 419, 428
HpyF3I CTNAG 4 cut(s) 122, 339, 561, 582
HpySE526I ACGT 3 cut(s) 375, 415, 480
HspAI GCGC 1 cut(s) 275
Kpn2I TCCGGA 1 cut(s) 206
Kzo9I GATC 1 cut(s) 133
Lsp1109I GCAGC 4 cut(s) 198, 318, 409, 422
LweI GCATC 1 cut(s) 232
MabI ACCWGGT 2 cut(s) 211, 592
MaeI CTAG 1 cut(s) 266
MaeII ACGT 3 cut(s) 375, 415, 480
MaeIII GTNAC 2 cut(s) 83, 481
MalI GATC 1 cut(s) 135
MboI GATC 1 cut(s) 133
MboII GAAGA 4 cut(s) 36, 39, 241, 609
MfeI CAATTG 2 cut(s) 321, 630
MflI RGATCY 1 cut(s) 133
MhlI GDGCHC 1 cut(s) 265
MluCI AATT 4 cut(s) 288, 321, 438, 630
MlyI GAGTC 1 cut(s) 302
MmeI TCCRAC 1 cut(s) 533
MroI TCCGGA 1 cut(s) 206
MroXI GAANNNNTTC 1 cut(s) 442
MspA1I CMGCKG 1 cut(s) 81
MspI CCGG 1 cut(s) 207
MspR9I CCNGG 2 cut(s) 213, 594
MunI CAATTG 2 cut(s) 321, 630
MvaI CCWGG 2 cut(s) 213, 594
MwoI GCNNNNNNNGC 7 cut(s) 87, 283, 340, 349, 395, 419, 428
NdeII GATC 1 cut(s) 133
NlaIV GGNNCC 1 cut(s) 627
NmuCI GTSAC 1 cut(s) 481
PdmI GAANNNNTTC 1 cut(s) 442
PkrI GCNGC 4 cut(s) 188, 333, 412, 424
PleI GAGTC 1 cut(s) 301
PmaCI CACGTG 1 cut(s) 416
PmlI CACGTG 1 cut(s) 416
PpsI GAGTC 1 cut(s) 301
Ppu21I YACGTR 1 cut(s) 416
PpuMI RGGWCCY 1 cut(s) 596
Psp124BI GAGCTC 1 cut(s) 265
Psp5II RGGWCCY 1 cut(s) 596
Psp6I CCWGG 2 cut(s) 211, 592
PspCI CACGTG 1 cut(s) 416
PspGI CCWGG 2 cut(s) 211, 592
PspN4I GGNNCC 1 cut(s) 627
PspPI GGNCC 3 cut(s) 209, 382, 596
PspPPI RGGWCCY 1 cut(s) 596
PstI CTGCAG 1 cut(s) 427
PstNI CAGNNNCTG 1 cut(s) 599
PsuI RGATCY 1 cut(s) 133
PvuII CAGCTG 1 cut(s) 81
RsaI GTAC 2 cut(s) 319, 378
RsaNI GTAC 2 cut(s) 318, 377
SacI GAGCTC 1 cut(s) 265
SatI GCNGC 4 cut(s) 187, 332, 411, 423
Sau3AI GATC 1 cut(s) 133
Sau96I GGNCC 3 cut(s) 209, 382, 596
SchI GAGTC 1 cut(s) 302
ScrFI CCNGG 2 cut(s) 213, 594
SduI GDGCHC 1 cut(s) 265
SexAI ACCWGGT 2 cut(s) 211, 592
SfaNI GCATC 1 cut(s) 232
SfcI CTRYAG 2 cut(s) 423, 456
SinI GGWCC 3 cut(s) 209, 382, 596
Sse9I AATT 4 cut(s) 288, 321, 438, 630
SsiI CCGC 1 cut(s) 269
SspMI CTAG 1 cut(s) 266
SstI GAGCTC 1 cut(s) 265
StyD4I CCNGG 2 cut(s) 211, 592
TaaI ACNGT 1 cut(s) 526
TaiI ACGT 3 cut(s) 378, 418, 483
TaqII GACCGA 1 cut(s) 399
TasI AATT 4 cut(s) 288, 321, 438, 630
TatI WGTACW 1 cut(s) 317
TscAI CASTG 3 cut(s) 115, 203, 304
TseFI GTSAC 1 cut(s) 481
TseI GCWGC 4 cut(s) 186, 331, 410, 422
Tsp45I GTSAC 1 cut(s) 481
TspDTI ATGAA 3 cut(s) 39, 543, 603
TspGWI ACGGA 1 cut(s) 94
TspRI CASTG 3 cut(s) 115, 203, 304
VpaK11BI GGWCC 3 cut(s) 209, 382, 596
XmnI GAANNNNTTC 1 cut(s) 442
XspI CTAG 1 cut(s) 266
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.