MD05G1177500.v1.1

protein At4g13200, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0012498
Physical Location & Seq
Reverse (-)
30450930 .. 30452649
1720 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1177500.v1.1.491

Sequence Viewer

Length: 639 bp
ATGATTAGTGGGGTTTTAACTTCTGCTTCTGCTTCCCCACAAGCACTTTCTTCCCTGCTTTCTAAACCCAGAAGCAGCAGCTTTTTCTCTGCGTCGTCTTCAAGCTCTGCAAGTGTTCATACAGATTTGAAGCTTAAAAGCGTTGGATTTTATGGAAGAATTGGGTCTCAGAGAAGCAGATTGGACTGCAGTGCAAATCCTGGTGGGCCCGGATTCGGTGAGGGTGATAGCAGGAGTGTTCTGGATGCCTTTTTCTTGGGGAAAGCTCTAGCTGAAGCTATAAACGACCGCATTGAGTCCGGTCTAGGCGAGTTTTTGAGCACGATTGGTAGGCTGCAAGCTGAGCAGCAGAAGCTTGTAGAGGAGTTTCAGGAAGATGTACTGGAAAGAGCCAAAAAGGCCAAAGAGAAAGCAGCACGCGAGGCCGTGGAGGCACAAGGAATCGTGACATCAACTACAGAATCCATAACACCAGTCACAACCACCAACGGCGTTACAGCAGTATCTTCGACAACCACCACGACTGTGGTCGCTCCTGTGAATCAATCCTCATTGTCTGAACCAGCTCCCTCAACAACTAAGGACGAACAAGATCCGGGTGACGATGACACGGTTCGAGGGGTGCCAATTGATGATTGA

Protein Analysis

213

Amino Acids

22.08

Weight (kDa)

4.79

Isoelectric Point (pI)

46.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF6825 PF20711 77 - 170 5.5e-32 Family of unknown function (DUF6825)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012498)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13200
fragaria_vesca FvH4_2g19250
malus_domestica MD05G1177500.v1.1 MD10G1165900.v1.1
prunus_persica Prupe.8G204400_v2.0.a1
pyrus_communis pycom05g16340 pycom05g16390 pycom10g14310
rosa_chinensis RchiOBHm_Chr6g0285061
rosa_laevigata RLG00000012681
rosa_multiflora Rmu_sc0002478.1_g000008
rosa_roxburghii Rroxscaffold_7G00183770 Rroxscaffold_7G00184070
rosa_rugosa Rorug06G0170400
rosa_samantha Rh6AG282100 Rh6BG282600 Rh6CG284600 Rh6DG277800
rosa_wichuraiana Rw6G024230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 622
AccII CGCG 1 cut(s) 420
AciI CCGC 1 cut(s) 289
AclWI GGATC 1 cut(s) 587
AcuI CTGAAG 1 cut(s) 294
AfaI GTAC 1 cut(s) 381
AfiI CCNNNNNNNGG 1 cut(s) 215
AgsI TTSAA 2 cut(s) 102, 130
AjnI CCWGG 1 cut(s) 199
AleI CACNNNNGTG 1 cut(s) 524
AluBI AGCT 9 cut(s) 81, 105, 133, 266, 272, 278, 341, 355, 566
AluI AGCT 9 cut(s) 81, 105, 133, 266, 272, 278, 341, 355, 566
Alw21I GWGCWC 1 cut(s) 323
Alw26I GTCTC 1 cut(s) 171
AlwI GGATC 1 cut(s) 587
AoxI GGCC 3 cut(s) 206, 399, 423
ApaI GGGCCC 1 cut(s) 210
ApeKI GCWGC 5 cut(s) 75, 78, 334, 346, 413
AspS9I GGNCC 2 cut(s) 206, 207
AsuC2I CCSGG 2 cut(s) 210, 597
AsuHPI GGTGA 3 cut(s) 230, 236, 611
BaeGI GKGCMC 1 cut(s) 210
BaeI ACNNNNGTAYC 2 cut(s) 486, 519
BanI GGYRCC 1 cut(s) 622
BanII GRGCYC 1 cut(s) 210
BbsI GAAGAC 1 cut(s) 90
Bbv12I GWGCWC 1 cut(s) 323
BbvI GCAGC 5 cut(s) 87, 90, 321, 358, 425
BceAI ACGGC 2 cut(s) 410, 505
BcgI CGANNNNNNTGC 2 cut(s) 489, 523
BciT130I CCWGG 1 cut(s) 201
BcnI CCSGG 2 cut(s) 210, 597
BcoDI GTCTC 1 cut(s) 171
BfaI CTAG 2 cut(s) 269, 305
BfmI CTRYAG 2 cut(s) 187, 456
BglI GCCNNNNNGGC 2 cut(s) 398, 431
BisI GCNGC 5 cut(s) 76, 79, 335, 347, 414
BlpI GCTNAGC 1 cut(s) 342
BlsI GCNGC 5 cut(s) 77, 80, 336, 348, 415
Bme1390I CCNGG 3 cut(s) 201, 210, 597
BmgT120I GGNCC 2 cut(s) 206, 207
BmiI GGNNCC 2 cut(s) 208, 624
BmrFI CCNGG 3 cut(s) 201, 210, 597
BmsI GCATC 1 cut(s) 235
BoxI GACNNNNGTC 1 cut(s) 527
BpiI GAAGAC 1 cut(s) 90
Bpu1102I GCTNAGC 1 cut(s) 342
BpuMI CCSGG 2 cut(s) 210, 597
BsaI GGTCTC 1 cut(s) 171
BsaJI CCNNGG 1 cut(s) 426
BsaWI WCCGGW 1 cut(s) 299
Bsc4I CCNNNNNNNGG 1 cut(s) 215
Bse1I ACTGG 2 cut(s) 387, 473
BseBI CCWGG 1 cut(s) 201
BseDI CCNNGG 1 cut(s) 426
BseGI GGATG 1 cut(s) 250
BseLI CCNNNNNNNGG 1 cut(s) 215
BseMII CTCAG 2 cut(s) 182, 333
BseNI ACTGG 2 cut(s) 387, 473
BseRI GAGGAG 1 cut(s) 377
BseSI GKGCMC 1 cut(s) 210
BseXI GCAGC 5 cut(s) 87, 90, 321, 358, 425
Bsh1236I CGCG 1 cut(s) 420
Bsh1285I CGRYCG 1 cut(s) 289
BshFI GGCC 3 cut(s) 208, 401, 425
BshNI GGYRCC 1 cut(s) 622
BsiEI CGRYCG 1 cut(s) 289
BsiHKAI GWGCWC 1 cut(s) 323
BsiSI CCGG 3 cut(s) 210, 300, 596
BslI CCNNNNNNNGG 1 cut(s) 215
BsmAI GTCTC 1 cut(s) 171
BsnI GGCC 3 cut(s) 208, 401, 425
Bso31I GGTCTC 1 cut(s) 171
Bsp120I GGGCCC 1 cut(s) 206
Bsp1286I GDGCHC 2 cut(s) 210, 323
Bsp143I GATC 1 cut(s) 592
Bsp1720I GCTNAGC 1 cut(s) 342
BspACI CCGC 1 cut(s) 289
BspANI GGCC 3 cut(s) 208, 401, 425
BspCNI CTCAG 2 cut(s) 181, 334
BspFNI CGCG 1 cut(s) 420
BspLI GGNNCC 2 cut(s) 208, 624
BspMAI CTGCAG 1 cut(s) 191
BspPI GGATC 1 cut(s) 587
BspT107I GGYRCC 1 cut(s) 622
BspTNI GGTCTC 1 cut(s) 171
BsrI ACTGG 2 cut(s) 387, 473
BssECI CCNNGG 1 cut(s) 426
BssMI GATC 1 cut(s) 592
Bst2UI CCWGG 1 cut(s) 201
Bst4CI ACNGT 2 cut(s) 526, 613
BstC8I GCNNGC 2 cut(s) 339, 418
BstDEI CTNAG 3 cut(s) 168, 342, 579
BstDSI CCRYGG 1 cut(s) 426
BstF5I GGATG 1 cut(s) 250
BstFNI CGCG 1 cut(s) 420
BstKTI GATC 1 cut(s) 595
BstMAI GTCTC 1 cut(s) 171
BstMBI GATC 1 cut(s) 592
BstMCI CGRYCG 1 cut(s) 289
BstMWI GCNNNNNNNGC 5 cut(s) 343, 352, 398, 422, 431
BstNI CCWGG 1 cut(s) 201
BstPAI GACNNNNGTC 1 cut(s) 527
BstSCI CCNGG 3 cut(s) 199, 208, 595
BstSFI CTRYAG 2 cut(s) 187, 456
BstSLI GKGCMC 1 cut(s) 210
BstUI CGCG 1 cut(s) 420
BstV1I GCAGC 5 cut(s) 87, 90, 321, 358, 425
BstV2I GAAGAC 1 cut(s) 90
BstX2I RGATCY 1 cut(s) 592
BstXI CCANNNNNNTGG 1 cut(s) 526
BstYI RGATCY 1 cut(s) 592
BsuRI GGCC 3 cut(s) 208, 401, 425
BtgI CCRYGG 1 cut(s) 426
BtsCI GGATG 1 cut(s) 250
BtsI GCAGTG 1 cut(s) 196
BtsIMutI CAGTG 1 cut(s) 196
Cac8I GCNNGC 2 cut(s) 339, 418
Cfr13I GGNCC 2 cut(s) 206, 207
CseI GACGC 1 cut(s) 81
Csp6I GTAC 1 cut(s) 380
CviQI GTAC 1 cut(s) 380
DdeI CTNAG 3 cut(s) 168, 342, 579
DpnI GATC 1 cut(s) 594
DpnII GATC 1 cut(s) 592
Eco24I GRGCYC 1 cut(s) 210
Eco31I GGTCTC 1 cut(s) 171
Eco57I CTGAAG 1 cut(s) 294
EcoRII CCWGG 1 cut(s) 199
EcoT38I GRGCYC 1 cut(s) 210
FaiI YATR 4 cut(s) 120, 153, 281, 467
Fnu4HI GCNGC 5 cut(s) 76, 79, 335, 347, 414
FokI GGATG 1 cut(s) 257
FriOI GRGCYC 1 cut(s) 210
Fsp4HI GCNGC 5 cut(s) 76, 79, 335, 347, 414
FspBI CTAG 2 cut(s) 269, 305
GluI GCNGC 5 cut(s) 76, 79, 335, 347, 414
HaeIII GGCC 3 cut(s) 208, 401, 425
HapII CCGG 3 cut(s) 210, 300, 596
HgaI GACGC 1 cut(s) 81
HindIII AAGCTT 2 cut(s) 131, 353
HinfI GANTC 5 cut(s) 213, 296, 441, 461, 541
HpaII CCGG 3 cut(s) 210, 300, 596
HphI GGTGA 3 cut(s) 230, 236, 611
Hpy188I TCNGA 2 cut(s) 171, 559
Hpy188III TCNNGA 3 cut(s) 242, 371, 445
Hpy99I CGWCG 1 cut(s) 97
HpyCH4III ACNGT 2 cut(s) 526, 613
HpyCH4V TGCA 4 cut(s) 110, 189, 194, 337
HpyF10VI GCNNNNNNNGC 5 cut(s) 343, 352, 398, 422, 431
HpyF3I CTNAG 3 cut(s) 168, 342, 579
Kzo9I GATC 1 cut(s) 592
LmnI GCTCC 2 cut(s) 538, 571
Lsp1109I GCAGC 5 cut(s) 87, 90, 321, 358, 425
LweI GCATC 1 cut(s) 235
MaeI CTAG 2 cut(s) 269, 305
MaeIII GTNAC 4 cut(s) 445, 475, 493, 599
MalI GATC 1 cut(s) 594
MboI GATC 1 cut(s) 592
MboII GAAGA 5 cut(s) 42, 90, 168, 386, 498
MfeI CAATTG 1 cut(s) 627
MflI RGATCY 1 cut(s) 592
MhlI GDGCHC 2 cut(s) 210, 323
MluCI AATT 2 cut(s) 159, 627
MlyI GAGTC 1 cut(s) 305
MmeI TCCRAC 1 cut(s) 124
MnlI CCTC 7 cut(s) 214, 355, 415, 424, 559, 580, 611
MseI TTAA 2 cut(s) 17, 135
MslI CAYNNNNRTG 1 cut(s) 524
MspI CCGG 3 cut(s) 210, 300, 596
MspR9I CCNGG 3 cut(s) 201, 210, 597
MunI CAATTG 1 cut(s) 627
MvaI CCWGG 1 cut(s) 201
MvnI CGCG 1 cut(s) 420
MwoI GCNNNNNNNGC 5 cut(s) 343, 352, 398, 422, 431
NciI CCSGG 2 cut(s) 210, 597
NdeII GATC 1 cut(s) 592
NlaIV GGNNCC 2 cut(s) 208, 624
NmuCI GTSAC 3 cut(s) 445, 475, 599
OliI CACNNNNGTG 1 cut(s) 524
PfeI GAWTC 4 cut(s) 213, 441, 461, 541
PkrI GCNGC 5 cut(s) 77, 80, 336, 348, 415
PleI GAGTC 1 cut(s) 304
PpsI GAGTC 1 cut(s) 304
PshAI GACNNNNGTC 1 cut(s) 527
Psp6I CCWGG 1 cut(s) 199
PspGI CCWGG 1 cut(s) 199
PspN4I GGNNCC 2 cut(s) 208, 624
PspOMI GGGCCC 1 cut(s) 206
PspPI GGNCC 2 cut(s) 206, 207
PstI CTGCAG 1 cut(s) 191
PsuI RGATCY 1 cut(s) 592
RsaI GTAC 1 cut(s) 381
RsaNI GTAC 1 cut(s) 380
RseI CAYNNNNRTG 1 cut(s) 524
SaqAI TTAA 2 cut(s) 17, 135
SatI GCNGC 5 cut(s) 76, 79, 335, 347, 414
Sau3AI GATC 1 cut(s) 592
Sau96I GGNCC 2 cut(s) 206, 207
SchI GAGTC 1 cut(s) 305
ScrFI CCNGG 3 cut(s) 201, 210, 597
SduI GDGCHC 2 cut(s) 210, 323
SetI ASST 9 cut(s) 83, 107, 135, 268, 274, 280, 343, 357, 568
SfaNI GCATC 1 cut(s) 235
SfcI CTRYAG 2 cut(s) 187, 456
SmiMI CAYNNNNRTG 1 cut(s) 524
Sse9I AATT 2 cut(s) 159, 627
SsiI CCGC 1 cut(s) 289
SspMI CTAG 2 cut(s) 269, 305
StyD4I CCNGG 3 cut(s) 199, 208, 595
TaaI ACNGT 2 cut(s) 526, 613
TaqI TCGA 2 cut(s) 509, 616
TasI AATT 2 cut(s) 159, 627
TatI WGTACW 1 cut(s) 379
TfiI GAWTC 4 cut(s) 213, 441, 461, 541
Tru1I TTAA 2 cut(s) 17, 135
Tru9I TTAA 2 cut(s) 17, 135
TscAI CASTG 1 cut(s) 196
TseFI GTSAC 3 cut(s) 445, 475, 599
TseI GCWGC 5 cut(s) 75, 78, 334, 346, 413
Tsp45I GTSAC 3 cut(s) 445, 475, 599
TspDTI ATGAA 1 cut(s) 107
TspRI CASTG 1 cut(s) 196
XcmI CCANNNNNNNNNTGG 1 cut(s) 523
XspI CTAG 2 cut(s) 269, 305
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.