Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0014058

Physical Location & Seq

Genomic Coordinates Reverse (-)

8306590 .. 8307934

Gene Structure 1345 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT4G14440.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 717 bp

ATGTGTACGTTAGAGAAGCGCGGCGATCTCTTTCTCCTAACCCTAACCGGCGAAGACGAGCACAGATTCCACCCCGACACGATCGCCTCTGTTCTCTCGCTTCTTGAACAAGCAAAGTCTCAATCCACAAAAGGATCCGTCCTCATCACTACCGGTCACGGAAAGTTCTTCTCCAATGGCTTTGACCTCGCGTGGGCTCAATCCGCTGGACACGGTGCTATAAAACGGATGCACCAAATGGTCAAATCCTTTAAACCGGTGTTAGCCGCCCTCCTCGATCTTCCAATGCCGACGATCGCCGCCTTGAACGGCCACGCGGCAGCCTCGGGGCTGATGTTTGCGCTGAGCCACGACTACGTTTTCATGAGGAAAGACCGTGGGGTTCTATATATGAGCGAGGTGGACATCGGGCTACCGGTGCCGGACTACTTCTCGGCGTTGGTCGTGGCTAAGGTTGGGTCAGGTATCGCGAGAAGGGAGCTGTTACTGAGCGGGAAGAAGCTCAAAGGAGAAGAAGCGGTGGCTCTCGGGATCGTGGACTCAGCGGCGCATGATAGTGCGGAAGGTGTGGTGGAGGCTACAGTGAGCCTTGGAGAAAGTTTGGCCGCTAAGAAATGGAATGGTGAGGTGTATGCAACCATTAGGAAGAGTTTGTATCCGGAGCTTTGTCGGATGGTTGATTTAACGGCAAATAACTTGGCCACTCACAACCTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004165 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005777 GO:0005829 GO:0006082 GO:0006629 GO:0006631 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009062 GO:0009628 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009850 GO:0009888 GO:0009987 GO:0010015 GO:0010033 GO:0010053 GO:0010054 GO:0010243 GO:0010817 GO:0014070 GO:0016042 GO:0016049 GO:0016054 GO:0016853 GO:0016860 GO:0016863 GO:0019752 GO:0021700 GO:0022622 GO:0030154 GO:0032501 GO:0032502 GO:0032787 GO:0034641 GO:0040007 GO:0042221 GO:0042430 GO:0042445 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044281 GO:0044282 GO:0044424 GO:0044444 GO:0044464 GO:0046395 GO:0046483 GO:0048364 GO:0048468 GO:0048469 GO:0048588 GO:0048589 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050896 GO:0060560 GO:0065007 GO:0065008 GO:0071695 GO:0071704 GO:0072329 GO:0080024 GO:0080026 GO:0080147 GO:0080167 GO:0090558 GO:0090627 GO:0099402 GO:1901360 GO:1901564 GO:1901575 GO:1901698 GO:1901700 GO:1905392

KEGG Pathways

Metabolic & Signaling

ko00062 ko01110 ko04626 map00062 map01110 map04626

Pfam Domains

Protein Families

ECH_1

Protein Analysis

238

Amino Acids

25.6

Weight (kDa)

6.75

Isoelectric Point (pI)

26.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ECH_1	PF00378	24 - 205	2.1e-22	Enoyl-CoA hydratase/isomerase
ECH_2	PF16113	26 - 194	6e-09	Enoyl-CoA hydratase/isomerase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014058)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G14430 AT4G14440
fragaria_vesca	FvH4_4g03200
malus_domestica	MD13G1210400.v1.1
prunus_persica	Prupe.1G034900_v2.0.a1
pyrus_communis	pycom13g18290
rosa_chinensis	RchiOBHm_Chr4g0391851
rosa_laevigata	RLG00000009854
rosa_multiflora	Rmu_sc0000383.1_g000017 Rmu_sc0007693.1_g000007
rosa_roxburghii	Rroxscaffold_5G00337180
rosa_rugosa	Rorug03G0336500
rosa_samantha	Rh4AG038900 Rh4BG033500 Rh4CG041500 Rh4DG036400
rosa_wichuraiana	Rw4G003030

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	418
AccBSI	CCGCTC	1 cut(s)	492
AccII	CGCG	4 cut(s)	21, 191, 317, 470
AccIII	TCCGGA	1 cut(s)	658
AclWI	GGATC	3 cut(s)	129, 142, 539
AcoI	YGGCCR	3 cut(s)	310, 603, 699
AfaI	GTAC	1 cut(s)	7
AfiI	CCNNNNNNNGG	1 cut(s)	193
AgeI	ACCGGT	3 cut(s)	152, 256, 415
AgsI	TTSAA	2 cut(s)	107, 307
AluBI	AGCT	3 cut(s)	481, 502, 664
AluI	AGCT	3 cut(s)	481, 502, 664
Alw21I	GWGCWC	1 cut(s)	63
Alw26I	GTCTC	1 cut(s)	123
AlwI	GGATC	3 cut(s)	129, 142, 539
Ama87I	CYCGRG	2 cut(s)	325, 527
Aor13HI	TCCGGA	1 cut(s)	658
AoxI	GGCC	3 cut(s)	310, 603, 699
ApeKI	GCWGC	1 cut(s)	320
AsiGI	ACCGGT	3 cut(s)	152, 256, 415
AspLEI	GCGC	3 cut(s)	21, 343, 550
AsuHPI	GGTGA	1 cut(s)	635
AvaI	CYCGRG	2 cut(s)	325, 527
BalI	TGGCCA	1 cut(s)	701
BamHI	GGATCC	1 cut(s)	134
BanI	GGYRCC	1 cut(s)	418
BanII	GRGCYC	1 cut(s)	199
BbsI	GAAGAC	1 cut(s)	60
Bbv12I	GWGCWC	1 cut(s)	63
BbvI	GCAGC	1 cut(s)	332
BccI	CCATC	1 cut(s)	667
BceAI	ACGGC	2 cut(s)	325, 702
BciVI	GTATCC	1 cut(s)	666
BcoDI	GTCTC	1 cut(s)	123
BfaI	CTAG	1 cut(s)	715
BfmI	CTRYAG	1 cut(s)	579
BfuI	GTATCC	1 cut(s)	666
BisI	GCNGC	7 cut(s)	22, 267, 300, 318, 321, 546, 606
BlpI	GCTNAGC	1 cut(s)	344
BlsI	GCNGC	7 cut(s)	23, 268, 301, 319, 322, 547, 607
BmeT110I	CYCGRG	2 cut(s)	325, 527
BmiI	GGNNCC	2 cut(s)	136, 420
BmsI	GCATC	1 cut(s)	219
BpiI	GAAGAC	1 cut(s)	60
Bpu10I	CCTNAGC	1 cut(s)	450
Bpu1102I	GCTNAGC	1 cut(s)	344
BsaJI	CCNNGG	3 cut(s)	324, 376, 589
BsaWI	WCCGGW	4 cut(s)	152, 256, 415, 658
Bsc4I	CCNNNNNNNGG	1 cut(s)	193
Bse118I	RCCGGY	4 cut(s)	47, 152, 256, 415
BseAI	TCCGGA	1 cut(s)	658
BseDI	CCNNGG	3 cut(s)	324, 376, 589
BseGI	GGATG	2 cut(s)	234, 678
BseLI	CCNNNNNNNGG	1 cut(s)	193
BseMII	CTCAG	3 cut(s)	335, 479, 555
BseRI	GAGGAG	1 cut(s)	263
BseXI	GCAGC	1 cut(s)	332
Bsh1236I	CGCG	4 cut(s)	21, 191, 317, 470
Bsh1285I	CGRYCG	2 cut(s)	84, 297
BshFI	GGCC	3 cut(s)	312, 605, 701
BshNI	GGYRCC	1 cut(s)	418
BshTI	ACCGGT	3 cut(s)	152, 256, 415
BsiEI	CGRYCG	2 cut(s)	84, 297
BsiHKAI	GWGCWC	1 cut(s)	63
BsiHKCI	CYCGRG	2 cut(s)	325, 527
BsiSI	CCGG	6 cut(s)	48, 153, 257, 416, 422, 659
BslI	CCNNNNNNNGG	1 cut(s)	193
BsmAI	GTCTC	1 cut(s)	123
BsnI	GGCC	3 cut(s)	312, 605, 701
BsoBI	CYCGRG	2 cut(s)	325, 527
Bsp1286I	GDGCHC	2 cut(s)	63, 199
Bsp13I	TCCGGA	1 cut(s)	658
Bsp143I	GATC	6 cut(s)	25, 81, 134, 277, 294, 531
Bsp1720I	GCTNAGC	1 cut(s)	344
Bsp68I	TCGCGA	1 cut(s)	470
BspANI	GGCC	3 cut(s)	312, 605, 701
BspCNI	CTCAG	3 cut(s)	336, 480, 554
BspEI	TCCGGA	1 cut(s)	658
BspFNI	CGCG	4 cut(s)	21, 191, 317, 470
BspHI	TCATGA	1 cut(s)	363
BspLI	GGNNCC	2 cut(s)	136, 420
BspPI	GGATC	3 cut(s)	129, 142, 539
BspT107I	GGYRCC	1 cut(s)	418
BsrBI	CCGCTC	1 cut(s)	492
BsrFI	RCCGGY	4 cut(s)	47, 152, 256, 415
BssAI	RCCGGY	4 cut(s)	47, 152, 256, 415
BssECI	CCNNGG	3 cut(s)	324, 376, 589
BssMI	GATC	6 cut(s)	25, 81, 134, 277, 294, 531
BssT1I	CCWWGG	1 cut(s)	589
Bst4CI	ACNGT	3 cut(s)	215, 377, 583
Bst6I	CTCTTC	1 cut(s)	641
BstDEI	CTNAG	5 cut(s)	344, 450, 488, 541, 609
BstDSI	CCRYGG	1 cut(s)	376
BstF5I	GGATG	2 cut(s)	234, 678
BstFNI	CGCG	4 cut(s)	21, 191, 317, 470
BstHHI	GCGC	3 cut(s)	21, 343, 550
BstKTI	GATC	6 cut(s)	28, 84, 137, 280, 297, 534
BstMAI	GTCTC	1 cut(s)	123
BstMBI	GATC	6 cut(s)	25, 81, 134, 277, 294, 531
BstMCI	CGRYCG	2 cut(s)	84, 297
BstMWI	GCNNNNNNNGC	2 cut(s)	203, 418
BstSFI	CTRYAG	1 cut(s)	579
BstUI	CGCG	4 cut(s)	21, 191, 317, 470
BstV1I	GCAGC	1 cut(s)	332
BstV2I	GAAGAC	1 cut(s)	60
BstX2I	RGATCY	1 cut(s)	134
BstYI	RGATCY	1 cut(s)	134
BsuI	GTATCC	1 cut(s)	666
BsuRI	GGCC	3 cut(s)	312, 605, 701
BtgI	CCRYGG	1 cut(s)	376
BtsCI	GGATG	2 cut(s)	234, 678
BtsIMutI	CAGTG	1 cut(s)	588
BtuMI	TCGCGA	1 cut(s)	470
CciI	TCATGA	1 cut(s)	363
CfoI	GCGC	3 cut(s)	21, 343, 550
Cfr10I	RCCGGY	4 cut(s)	47, 152, 256, 415
Csp6I	GTAC	1 cut(s)	6
CspAI	ACCGGT	3 cut(s)	152, 256, 415
CviAII	CATG	2 cut(s)	364, 551
CviQI	GTAC	1 cut(s)	6
DdeI	CTNAG	5 cut(s)	344, 450, 488, 541, 609
DpnI	GATC	6 cut(s)	27, 83, 136, 279, 296, 533
DpnII	GATC	6 cut(s)	25, 81, 134, 277, 294, 531
DraI	TTTAAA	1 cut(s)	253
EaeI	YGGCCR	3 cut(s)	310, 603, 699
Eam1104I	CTCTTC	1 cut(s)	641
EarI	CTCTTC	1 cut(s)	641
Eco130I	CCWWGG	1 cut(s)	589
Eco24I	GRGCYC	1 cut(s)	199
Eco88I	CYCGRG	2 cut(s)	325, 527
EcoT14I	CCWWGG	1 cut(s)	589
EcoT38I	GRGCYC	1 cut(s)	199
ErhI	CCWWGG	1 cut(s)	589
FaeI	CATG	2 cut(s)	367, 554
FaiI	YATR	7 cut(s)	221, 365, 388, 390, 392, 552, 633
FatI	CATG	2 cut(s)	363, 550
FauI	CCCGC	1 cut(s)	485
Fnu4HI	GCNGC	7 cut(s)	22, 267, 300, 318, 321, 546, 606
FokI	GGATG	2 cut(s)	241, 685
FriOI	GRGCYC	1 cut(s)	199
Fsp4HI	GCNGC	7 cut(s)	22, 267, 300, 318, 321, 546, 606
FspBI	CTAG	1 cut(s)	715
GlaI	GCGC	3 cut(s)	20, 342, 549
GluI	GCNGC	7 cut(s)	22, 267, 300, 318, 321, 546, 606
HaeIII	GGCC	3 cut(s)	312, 605, 701
HapII	CCGG	6 cut(s)	48, 153, 257, 416, 422, 659
HhaI	GCGC	3 cut(s)	21, 343, 550
Hin1II	CATG	2 cut(s)	367, 554
Hin6I	GCGC	3 cut(s)	19, 341, 548
HinP1I	GCGC	3 cut(s)	19, 341, 548
HinfI	GANTC	2 cut(s)	66, 539
HpaII	CCGG	6 cut(s)	48, 153, 257, 416, 422, 659
HphI	GGTGA	1 cut(s)	635
Hpy166II	GTNNAC	3 cut(s)	6, 403, 538
Hpy188I	TCNGA	1 cut(s)	672
Hpy188III	TCNNGA	5 cut(s)	104, 364, 469, 529, 659
Hpy8I	GTNNAC	3 cut(s)	6, 403, 538
Hpy99I	CGWCG	1 cut(s)	295
HpyAV	CCTTC	2 cut(s)	468, 557
HpyCH4III	ACNGT	3 cut(s)	215, 377, 583
HpyCH4IV	ACGT	2 cut(s)	8, 357
HpyCH4V	TGCA	2 cut(s)	232, 635
HpyF10VI	GCNNNNNNNGC	2 cut(s)	203, 418
HpyF3I	CTNAG	5 cut(s)	344, 450, 488, 541, 609
HpySE526I	ACGT	2 cut(s)	8, 357
Hsp92II	CATG	2 cut(s)	367, 554
HspAI	GCGC	3 cut(s)	19, 341, 548
Kpn2I	TCCGGA	1 cut(s)	658
Kzo9I	GATC	6 cut(s)	25, 81, 134, 277, 294, 531
LmnI	GCTCC	2 cut(s)	478, 661
LpnPI	CCDG	8 cut(s)	61, 166, 192, 270, 429, 435, 447, 672
Lsp1109I	GCAGC	1 cut(s)	332
LweI	GCATC	1 cut(s)	219
MaeI	CTAG	1 cut(s)	715
MaeII	ACGT	2 cut(s)	8, 357
MaeIII	GTNAC	2 cut(s)	155, 483
MalI	GATC	6 cut(s)	27, 83, 136, 279, 296, 533
MbiI	CCGCTC	1 cut(s)	492
MboI	GATC	6 cut(s)	25, 81, 134, 277, 294, 531
MboII	GAAGA	6 cut(s)	65, 160, 272, 508, 524, 658
MflI	RGATCY	1 cut(s)	134
MhlI	GDGCHC	2 cut(s)	63, 199
MlsI	TGGCCA	1 cut(s)	701
MluNI	TGGCCA	1 cut(s)	701
MlyI	GAGTC	1 cut(s)	533
MmeI	TCCRAC	1 cut(s)	650
Mox20I	TGGCCA	1 cut(s)	701
MroI	TCCGGA	1 cut(s)	658
MscI	TGGCCA	1 cut(s)	701
MseI	TTAA	2 cut(s)	252, 683
MslI	CAYNNNNRTG	1 cut(s)	555
Msp20I	TGGCCA	1 cut(s)	701
MspA1I	CMGCKG	2 cut(s)	206, 545
MspI	CCGG	6 cut(s)	48, 153, 257, 416, 422, 659
MvnI	CGCG	4 cut(s)	21, 191, 317, 470
MwoI	GCNNNNNNNGC	2 cut(s)	203, 418
NdeII	GATC	6 cut(s)	25, 81, 134, 277, 294, 531
NlaIII	CATG	2 cut(s)	367, 554
NlaIV	GGNNCC	2 cut(s)	136, 420
NmeAIII	GCCGAG	1 cut(s)	413
NmuCI	GTSAC	1 cut(s)	155
NruI	TCGCGA	1 cut(s)	470
PagI	TCATGA	1 cut(s)	363
PfeI	GAWTC	1 cut(s)	66
PinAI	ACCGGT	3 cut(s)	152, 256, 415
PkrI	GCNGC	7 cut(s)	23, 268, 301, 319, 322, 547, 607
Ple19I	CGATCG	2 cut(s)	84, 297
PleI	GAGTC	1 cut(s)	533
PpsI	GAGTC	1 cut(s)	533
PspN4I	GGNNCC	2 cut(s)	136, 420
PsuI	RGATCY	1 cut(s)	134
PvuI	CGATCG	2 cut(s)	84, 297
RruI	TCGCGA	1 cut(s)	470
RsaI	GTAC	1 cut(s)	7
RsaNI	GTAC	1 cut(s)	6
RseI	CAYNNNNRTG	1 cut(s)	555
SaqAI	TTAA	2 cut(s)	252, 683
SatI	GCNGC	7 cut(s)	22, 267, 300, 318, 321, 546, 606
Sau3AI	GATC	6 cut(s)	25, 81, 134, 277, 294, 531
SchI	GAGTC	1 cut(s)	533
SduI	GDGCHC	2 cut(s)	63, 199
SfaNI	GCATC	1 cut(s)	219
SfcI	CTRYAG	1 cut(s)	579
SmiMI	CAYNNNNRTG	1 cut(s)	555
SspMI	CTAG	1 cut(s)	715
StyI	CCWWGG	1 cut(s)	589
TaaI	ACNGT	3 cut(s)	215, 377, 583
TaiI	ACGT	2 cut(s)	11, 360
TaqI	TCGA	1 cut(s)	276
TauI	GCSGC	6 cut(s)	24, 269, 302, 320, 548, 608
TfiI	GAWTC	1 cut(s)	66
Tru1I	TTAA	2 cut(s)	252, 683
Tru9I	TTAA	2 cut(s)	252, 683
TscAI	CASTG	1 cut(s)	588
TseFI	GTSAC	1 cut(s)	155
TseI	GCWGC	1 cut(s)	320
Tsp45I	GTSAC	1 cut(s)	155
TspDTI	ATGAA	1 cut(s)	352
TspGWI	ACGGA	3 cut(s)	127, 174, 241
TspRI	CASTG	1 cut(s)	588
XspI	CTAG	1 cut(s)	715

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.