Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0014058

Physical Location & Seq

Genomic Coordinates Forward (+)

19584335 .. 19585051

Gene Structure 717 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1210400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 717 bp

ATGTGCACATTAGAGAAACGCGGCGATCTCTTCTTCCTAACCCTCACCGGAGACGACGACCACCGCCTCGGCCTCCCAATCATCGACTCCCTCCTCTCCGCCATCGCCGAAGCCAAGTCCCAAGCCACGCGCGGCTCCGTCTTCATCACCACCGCCCAAGGCAAGTTCTTCTCCAATGGCTTCGACCTCGGCTGGGCCCAATCCGCCGGCTCCGCCACCGCTGCCCGCGCCCGCCTCAATCAAATGGTCCTCGCCTTCAAGCCGGTCGTCGTCGCGCTCCTCTCGCTCCCGATGCCGACGATCGCCGCAGTCCAAGGCCACGCCGCCGCAGCCGGCTTCTTGTTCGCCCTGAGCCACGATTACTTCCTGATGAGGCGCGACAGAGGCGTGCTGTACATGAGCGAGGTCGATTTGGGGCTGCCGTTCCCGGACTACTTCACGGCTGCGTTTCGGTCGAAGATTGGGTCGGTGTCAGGTCGGAGGGATGTGATGCTGAGGGGGATGAAGGTGAAGGGAGAGGAGGCGGTGAAAATGGGGATCGTGGAGTCGGCGCACGATAGCGCGGAGAGCACGGTGGAGGCTGCGGTGCGCCTGGGGGAGGAGTTGGGGAAGAGGAAGTGGAACGGCGACGTTTACGCGGAGATCAGGAAGAGCTTGTATCCGGAGTTGTGTGGAGTTCTTGGAGTGGCGATTGCTACTCCAAAGGCAAAGCTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004165 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005777 GO:0005829 GO:0006082 GO:0006629 GO:0006631 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009062 GO:0009628 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009850 GO:0009888 GO:0009987 GO:0010015 GO:0010033 GO:0010053 GO:0010054 GO:0010243 GO:0010817 GO:0014070 GO:0016042 GO:0016049 GO:0016054 GO:0016853 GO:0016860 GO:0016863 GO:0019752 GO:0021700 GO:0022622 GO:0030154 GO:0032501 GO:0032502 GO:0032787 GO:0034641 GO:0040007 GO:0042221 GO:0042430 GO:0042445 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044281 GO:0044282 GO:0044424 GO:0044444 GO:0044464 GO:0046395 GO:0046483 GO:0048364 GO:0048468 GO:0048469 GO:0048588 GO:0048589 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050896 GO:0060560 GO:0065007 GO:0065008 GO:0071695 GO:0071704 GO:0072329 GO:0080024 GO:0080026 GO:0080147 GO:0080167 GO:0090558 GO:0090627 GO:0099402 GO:1901360 GO:1901564 GO:1901575 GO:1901698 GO:1901700 GO:1905392

KEGG Pathways

Metabolic & Signaling

ko00062 ko01110 ko04626 map00062 map01110 map04626

Pfam Domains

Protein Families

ECH_1

Protein Analysis

239

Amino Acids

25.65

Weight (kDa)

8.85

Isoelectric Point (pI)

36.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ECH_2	PF16113	23 - 190	2.2e-06	Enoyl-CoA hydratase/isomerase
ECH_1	PF00378	27 - 204	3.8e-19	Enoyl-CoA hydratase/isomerase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014058)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G14430 AT4G14440
fragaria_vesca	FvH4_4g03200
malus_domestica	MD13G1210400.v1.1
prunus_persica	Prupe.1G034900_v2.0.a1
pyrus_communis	pycom13g18290
rosa_chinensis	RchiOBHm_Chr4g0391851
rosa_laevigata	RLG00000009854
rosa_multiflora	Rmu_sc0000383.1_g000017 Rmu_sc0007693.1_g000007
rosa_roxburghii	Rroxscaffold_5G00337180
rosa_rugosa	Rorug03G0336500
rosa_samantha	Rh4AG038900 Rh4BG033500 Rh4CG041500 Rh4DG036400
rosa_wichuraiana	Rw4G003030

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	8 cut(s)	21, 130, 132, 228, 275, 378, 563, 638
AccIII	TCCGGA	1 cut(s)	661
AclWI	GGATC	1 cut(s)	545
AfaI	GTAC	1 cut(s)	395
AfiI	CCNNNNNNNGG	2 cut(s)	193, 598
AgsI	TTSAA	1 cut(s)	259
AjnI	CCWGG	1 cut(s)	591
AluBI	AGCT	2 cut(s)	654, 712
AluI	AGCT	2 cut(s)	654, 712
Alw21I	GWGCWC	2 cut(s)	8, 572
Alw26I	GTCTC	1 cut(s)	45
Alw44I	GTGCAC	1 cut(s)	4
AlwI	GGATC	1 cut(s)	545
Aor13HI	TCCGGA	1 cut(s)	661
AoxI	GGCC	3 cut(s)	70, 195, 316
ApaI	GGGCCC	1 cut(s)	199
ApaLI	GTGCAC	1 cut(s)	4
ApeKI	GCWGC	5 cut(s)	221, 329, 418, 443, 581
ArsI	GACNNNNNNTTYG	2 cut(s)	449, 481
AspLEI	GCGC	7 cut(s)	132, 230, 277, 378, 553, 563, 591
AspS9I	GGNCC	3 cut(s)	195, 196, 247
AsuC2I	CCSGG	1 cut(s)	428
AsuHPI	GGTGA	4 cut(s)	37, 139, 520, 538
AvaII	GGWCC	1 cut(s)	247
BaeGI	GKGCMC	2 cut(s)	8, 199
BanII	GRGCYC	1 cut(s)	199
BbsI	GAAGAC	1 cut(s)	133
Bbv12I	GWGCWC	2 cut(s)	8, 572
BbvCI	CCTCAGC	1 cut(s)	494
BbvI	GCAGC	5 cut(s)	208, 341, 405, 430, 568
BccI	CCATC	1 cut(s)	110
BceAI	ACGGC	3 cut(s)	406, 456, 640
BciT130I	CCWGG	1 cut(s)	593
BciVI	GTATCC	1 cut(s)	669
BcnI	CCSGG	1 cut(s)	428
BcoDI	GTCTC	1 cut(s)	45
BfuI	GTATCC	1 cut(s)	669
Bme1390I	CCNGG	2 cut(s)	428, 593
Bme18I	GGWCC	1 cut(s)	247
BmgT120I	GGNCC	3 cut(s)	195, 196, 247
BmiI	GGNNCC	3 cut(s)	136, 197, 211
BmrFI	CCNGG	2 cut(s)	428, 593
BmsI	GCATC	2 cut(s)	282, 480
BpiI	GAAGAC	1 cut(s)	133
Bpu10I	CCTNAGC	2 cut(s)	350, 494
BpuMI	CCSGG	1 cut(s)	428
BsaJI	CCNNGG	5 cut(s)	67, 157, 187, 313, 592
BsaWI	WCCGGW	2 cut(s)	47, 661
Bsc4I	CCNNNNNNNGG	2 cut(s)	193, 598
Bse118I	RCCGGY	3 cut(s)	206, 262, 332
BseAI	TCCGGA	1 cut(s)	661
BseBI	CCWGG	1 cut(s)	593
BseDI	CCNNGG	5 cut(s)	67, 157, 187, 313, 592
BseGI	GGATG	2 cut(s)	490, 507
BseLI	CCNNNNNNNGG	2 cut(s)	193, 598
BseMII	CTCAG	2 cut(s)	341, 485
BseRI	GAGGAG	4 cut(s)	83, 269, 533, 614
BseSI	GKGCMC	2 cut(s)	8, 199
BseXI	GCAGC	5 cut(s)	208, 341, 405, 430, 568
BseYI	CCCAGC	1 cut(s)	192
Bsh1236I	CGCG	8 cut(s)	21, 130, 132, 228, 275, 378, 563, 638
Bsh1285I	CGRYCG	3 cut(s)	267, 303, 455
BshFI	GGCC	3 cut(s)	72, 197, 318
BsiEI	CGRYCG	3 cut(s)	267, 303, 455
BsiHKAI	GWGCWC	2 cut(s)	8, 572
BsiSI	CCGG	6 cut(s)	48, 207, 263, 333, 428, 662
BslFI	GGGAC	1 cut(s)	103
BslI	CCNNNNNNNGG	2 cut(s)	193, 598
BsmAI	GTCTC	1 cut(s)	45
BsmBI	CGTCTC	1 cut(s)	45
BsmFI	GGGAC	1 cut(s)	103
BsnI	GGCC	3 cut(s)	72, 197, 318
Bsp120I	GGGCCC	1 cut(s)	195
Bsp1286I	GDGCHC	3 cut(s)	8, 199, 572
Bsp13I	TCCGGA	1 cut(s)	661
Bsp1407I	TGTACA	1 cut(s)	393
Bsp143I	GATC	4 cut(s)	25, 300, 537, 642
BspANI	GGCC	3 cut(s)	72, 197, 318
BspCNI	CTCAG	2 cut(s)	342, 486
BspEI	TCCGGA	1 cut(s)	661
BspFNI	CGCG	8 cut(s)	21, 130, 132, 228, 275, 378, 563, 638
BspLI	GGNNCC	3 cut(s)	136, 197, 211
BspPI	GGATC	1 cut(s)	545
BspQI	GCTCTTC	1 cut(s)	644
BsrFI	RCCGGY	3 cut(s)	206, 262, 332
BsrGI	TGTACA	1 cut(s)	393
BssAI	RCCGGY	3 cut(s)	206, 262, 332
BssECI	CCNNGG	5 cut(s)	67, 157, 187, 313, 592
BssMI	GATC	4 cut(s)	25, 300, 537, 642
BssT1I	CCWWGG	2 cut(s)	157, 313
Bst2UI	CCWGG	1 cut(s)	593
Bst4CI	ACNGT	1 cut(s)	574
Bst6I	CTCTTC	3 cut(s)	35, 605, 644
BstAUI	TGTACA	1 cut(s)	393
BstC8I	GCNNGC	5 cut(s)	208, 226, 232, 334, 389
BstDEI	CTNAG	2 cut(s)	350, 494
BstENI	CCTNNNNNAGG	1 cut(s)	596
BstF5I	GGATG	2 cut(s)	490, 507
BstFNI	CGCG	8 cut(s)	21, 130, 132, 228, 275, 378, 563, 638
BstHHI	GCGC	7 cut(s)	132, 230, 277, 378, 553, 563, 591
BstKTI	GATC	4 cut(s)	28, 303, 540, 645
BstMAI	GTCTC	1 cut(s)	45
BstMBI	GATC	4 cut(s)	25, 300, 537, 642
BstMCI	CGRYCG	3 cut(s)	267, 303, 455
BstMWI	GCNNNNNNNGC	9 cut(s)	203, 212, 221, 227, 283, 292, 329, 384, 567
BstNI	CCWGG	1 cut(s)	593
BstSCI	CCNGG	2 cut(s)	426, 591
BstSLI	GKGCMC	2 cut(s)	8, 199
BstUI	CGCG	8 cut(s)	21, 130, 132, 228, 275, 378, 563, 638
BstV1I	GCAGC	5 cut(s)	208, 341, 405, 430, 568
BstV2I	GAAGAC	1 cut(s)	133
BsuI	GTATCC	1 cut(s)	669
BsuRI	GGCC	3 cut(s)	72, 197, 318
BtgZI	GCGATG	1 cut(s)	88
BtsCI	GGATG	2 cut(s)	490, 507
Cac8I	GCNNGC	5 cut(s)	208, 226, 232, 334, 389
CfoI	GCGC	7 cut(s)	132, 230, 277, 378, 553, 563, 591
Cfr10I	RCCGGY	3 cut(s)	206, 262, 332
Cfr13I	GGNCC	3 cut(s)	195, 196, 247
Csp6I	GTAC	1 cut(s)	394
CviAII	CATG	1 cut(s)	397
CviQI	GTAC	1 cut(s)	394
DdeI	CTNAG	2 cut(s)	350, 494
DpnI	GATC	4 cut(s)	27, 302, 539, 644
DpnII	GATC	4 cut(s)	25, 300, 537, 642
Eam1104I	CTCTTC	3 cut(s)	35, 605, 644
EarI	CTCTTC	3 cut(s)	35, 605, 644
EciI	GGCGGA	3 cut(s)	88, 193, 202
Eco130I	CCWWGG	2 cut(s)	157, 313
Eco24I	GRGCYC	1 cut(s)	199
Eco47I	GGWCC	1 cut(s)	247
EcoNI	CCTNNNNNAGG	1 cut(s)	596
EcoRII	CCWGG	1 cut(s)	591
EcoT14I	CCWWGG	2 cut(s)	157, 313
EcoT38I	GRGCYC	1 cut(s)	199
ErhI	CCWWGG	2 cut(s)	157, 313
Esp3I	CGTCTC	1 cut(s)	45
FaeI	CATG	1 cut(s)	400
FaiI	YATR	1 cut(s)	398
FaqI	GGGAC	1 cut(s)	103
FatI	CATG	1 cut(s)	396
FauI	CCCGC	2 cut(s)	233, 239
FokI	GGATG	2 cut(s)	497, 514
FriOI	GRGCYC	1 cut(s)	199
GlaI	GCGC	7 cut(s)	131, 229, 276, 377, 552, 562, 590
GsaI	CCCAGC	1 cut(s)	196
HaeIII	GGCC	3 cut(s)	72, 197, 318
HapII	CCGG	6 cut(s)	48, 207, 263, 333, 428, 662
HhaI	GCGC	7 cut(s)	132, 230, 277, 378, 553, 563, 591
Hin1II	CATG	1 cut(s)	400
Hin6I	GCGC	7 cut(s)	130, 228, 275, 376, 551, 561, 589
HinP1I	GCGC	7 cut(s)	130, 228, 275, 376, 551, 561, 589
HinfI	GANTC	2 cut(s)	86, 545
HpaII	CCGG	6 cut(s)	48, 207, 263, 333, 428, 662
HphI	GGTGA	4 cut(s)	37, 139, 520, 538
Hpy166II	GTNNAC	2 cut(s)	6, 634
Hpy188I	TCNGA	2 cut(s)	480, 716
Hpy188III	TCNNGA	4 cut(s)	289, 367, 646, 662
Hpy8I	GTNNAC	2 cut(s)	6, 634
Hpy99I	CGWCG	5 cut(s)	59, 272, 275, 301, 632
HpyAV	CCTTC	3 cut(s)	265, 499, 505
HpyCH4III	ACNGT	1 cut(s)	574
HpyCH4IV	ACGT	1 cut(s)	630
HpyCH4V	TGCA	1 cut(s)	6
HpyF10VI	GCNNNNNNNGC	9 cut(s)	203, 212, 221, 227, 283, 292, 329, 384, 567
HpyF3I	CTNAG	2 cut(s)	350, 494
HpySE526I	ACGT	1 cut(s)	630
Hsp92II	CATG	1 cut(s)	400
HspAI	GCGC	7 cut(s)	130, 228, 275, 376, 551, 561, 589
Kpn2I	TCCGGA	1 cut(s)	661
KroI	GCCGGC	2 cut(s)	206, 332
KroNI	GCCGGC	2 cut(s)	208, 334
Kzo9I	GATC	4 cut(s)	25, 300, 537, 642
LguI	GCTCTTC	1 cut(s)	644
LmnI	GCTCC	4 cut(s)	140, 215, 282, 291
Lsp1109I	GCAGC	5 cut(s)	208, 341, 405, 430, 568
LweI	GCATC	2 cut(s)	282, 480
MaeII	ACGT	1 cut(s)	630
MalI	GATC	4 cut(s)	27, 302, 539, 644
MboI	GATC	4 cut(s)	25, 300, 537, 642
MboII	GAAGA	7 cut(s)	22, 25, 133, 160, 469, 622, 661
MhlI	GDGCHC	3 cut(s)	8, 199, 572
MlyI	GAGTC	2 cut(s)	80, 554
MmeI	TCCRAC	1 cut(s)	458
MroI	TCCGGA	1 cut(s)	661
MroNI	GCCGGC	2 cut(s)	206, 332
MspA1I	CMGCKG	1 cut(s)	221
MspI	CCGG	6 cut(s)	48, 207, 263, 333, 428, 662
MspR9I	CCNGG	2 cut(s)	428, 593
MvaI	CCWGG	1 cut(s)	593
MvnI	CGCG	8 cut(s)	21, 130, 132, 228, 275, 378, 563, 638
MwoI	GCNNNNNNNGC	9 cut(s)	203, 212, 221, 227, 283, 292, 329, 384, 567
NaeI	GCCGGC	2 cut(s)	208, 334
NciI	CCSGG	1 cut(s)	428
NdeII	GATC	4 cut(s)	25, 300, 537, 642
NgoMIV	GCCGGC	2 cut(s)	206, 332
NlaIII	CATG	1 cut(s)	400
NlaIV	GGNNCC	3 cut(s)	136, 197, 211
NmeAIII	GCCGAG	2 cut(s)	48, 168
PciSI	GCTCTTC	1 cut(s)	644
PdiI	GCCGGC	2 cut(s)	208, 334
PfoI	TCCNGGA	1 cut(s)	426
Ple19I	CGATCG	1 cut(s)	303
PleI	GAGTC	2 cut(s)	80, 553
PpsI	GAGTC	2 cut(s)	80, 553
Psp6I	CCWGG	1 cut(s)	591
PspFI	CCCAGC	1 cut(s)	192
PspGI	CCWGG	1 cut(s)	591
PspN4I	GGNNCC	3 cut(s)	136, 197, 211
PspOMI	GGGCCC	1 cut(s)	195
PspPI	GGNCC	3 cut(s)	195, 196, 247
PvuI	CGATCG	1 cut(s)	303
RsaI	GTAC	1 cut(s)	395
RsaNI	GTAC	1 cut(s)	394
SapI	GCTCTTC	1 cut(s)	644
Sau3AI	GATC	4 cut(s)	25, 300, 537, 642
Sau96I	GGNCC	3 cut(s)	195, 196, 247
SchI	GAGTC	2 cut(s)	80, 554
ScrFI	CCNGG	2 cut(s)	428, 593
SduI	GDGCHC	3 cut(s)	8, 199, 572
SetI	ASST	7 cut(s)	189, 408, 478, 510, 633, 656, 714
SfaNI	GCATC	2 cut(s)	282, 480
SinI	GGWCC	1 cut(s)	247
StyD4I	CCNGG	2 cut(s)	426, 591
StyI	CCWWGG	2 cut(s)	157, 313
TaaI	ACNGT	1 cut(s)	574
TaiI	ACGT	1 cut(s)	633
TaqI	TCGA	4 cut(s)	84, 183, 408, 455
TaqII	GACCGA	1 cut(s)	441
TatI	WGTACW	1 cut(s)	393
TauI	GCSGC	5 cut(s)	24, 135, 308, 326, 329
TseI	GCWGC	5 cut(s)	221, 329, 418, 443, 581
TspDTI	ATGAA	2 cut(s)	133, 518
TspGWI	ACGGA	1 cut(s)	127
VneI	GTGCAC	1 cut(s)	4
VpaK11BI	GGWCC	1 cut(s)	247
XagI	CCTNNNNNAGG	1 cut(s)	596

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.