AT4G14965

Belongs to the cytochrome b5 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0013285
Physical Location & Seq
Forward (+)
8551244 .. 8553618
2375 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G14965.1

Sequence Viewer

Length: 738 bp
ATGATTCCGGCGAGGAGGTTTCTCTTATCTCCATTTGTCGGCGTCACGTTTATCGTCGTTCTCGTCTCTCTCTACTTCAGATCTTCCTTCAAGTCTCCACAACACCAATATCAAAAGAGGTTATTTTCTGCTGAAGAATTGGCATTGTATAATGGTACTGATGAAACATTACCTATACTCTTAGGGATTCTTGGATCTGTGTTCGATGTGACGAAAGGAAAATTTCATTACGGTAGTGGAGGAGGGTACAACCATTTTGCTGGAAGAGACGCTTCTCGTGCATTTGTTTCTGGCAACTTTACAGGAGATGGACTTACAGATTCGTTACAAGGGTTATCTAGCAGTGAGGTGAAGAGCATTGTTGACTGGCGAGGTTTCTACTCCAGGACCTACACTCCTGTTGGGAAGCTTGTTGGGCGGTACTACGATAGCCAAGGGAATCCAACTAAACACCTTAAAGGAGCTGAAGCAAAAGCCTCCAGAGGTGCACAACTCATGGAGAAACAGAAGACTGAAGAAGCCAAGCAATCGAATTGCAACTCACGATGGAGTCAAGATGAAGGTGGAGAGGTTTGGTGTGATGTTGGAGTGCCAAGACTGGTACAAAGACCTTTGGAGATAGCAATAACTGGCTCGATGAGCAAACGCTGTGCTTGCTTTGAGGAAGACCAACTTGATCAGTCTGGCTTGGAGATCTATAAAGACTGTGAACCTCTTGCCAAGACTTGCAGGGTTTGA

Protein Analysis

245

Amino Acids

27.17

Weight (kDa)

8.46

Isoelectric Point (pI)

52.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyt-b5 PF00173 44 - 103 4.3e-08 Cytochrome b5-like Heme/Steroid binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013285)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G14965 AT4G14965
fragaria_vesca FvH4_6g40100 FvH4_6g40100
malus_domestica MD09G1135300.v1.1
prunus_persica Prupe.3G191400_v2.0.a1
pyrus_communis pycom09g05640
rosa_chinensis RchiOBHm_Chr2g0154731
rosa_laevigata RLG00000020798
rosa_multiflora Rmu_sc0001004.1_g000005 Rmu_sc0001027.1_g000007
rosa_roxburghii Rroxscaffold_2G00094150
rosa_rugosa Rorug02G0444800
rosa_samantha Rh2AG510200 Rh2BG521100 Rh2CG495400 Rh2DG531200
rosa_wichuraiana Rw2G041960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 418
AclWI GGATC 1 cut(s) 202
AcsI RAATTY 1 cut(s) 221
AcuI CTGAAG 4 cut(s) 61, 153, 486, 534
AcyI GRCGYC 1 cut(s) 42
AfaI GTAC 4 cut(s) 157, 248, 422, 603
AfiI CCNNNNNNNGG 1 cut(s) 38
AgsI TTSAA 1 cut(s) 91
AjnI CCWGG 1 cut(s) 383
AluBI AGCT 2 cut(s) 409, 464
AluI AGCT 2 cut(s) 409, 464
Alw21I GWGCWC 1 cut(s) 490
Alw26I GTCTC 3 cut(s) 70, 99, 261
Alw44I GTGCAC 1 cut(s) 486
AlwI GGATC 1 cut(s) 202
ApaLI GTGCAC 1 cut(s) 486
ApoI RAATTY 1 cut(s) 221
AspS9I GGNCC 1 cut(s) 387
AsuHPI GGTGA 1 cut(s) 361
AvaII GGWCC 1 cut(s) 387
BaeGI GKGCMC 1 cut(s) 490
BauI CACGAG 1 cut(s) 276
BbsI GAAGAC 2 cut(s) 515, 672
Bbv12I GWGCWC 1 cut(s) 490
BccI CCATC 2 cut(s) 302, 540
BciT130I CCWGG 1 cut(s) 385
BclI TGATCA 1 cut(s) 676
BcoDI GTCTC 3 cut(s) 70, 99, 261
BfaI CTAG 1 cut(s) 339
BglII AGATCT 2 cut(s) 80, 693
Bme1390I CCNGG 1 cut(s) 385
Bme18I GGWCC 1 cut(s) 387
BmgT120I GGNCC 1 cut(s) 387
BmrFI CCNGG 1 cut(s) 385
BpiI GAAGAC 2 cut(s) 515, 672
BpmI CTGGAG 2 cut(s) 367, 463
BsaHI GRCGYC 1 cut(s) 42
BsaJI CCNNGG 1 cut(s) 433
BsaXI ACNNNNNCTCC 2 cut(s) 379, 409
Bsc4I CCNNNNNNNGG 1 cut(s) 38
Bse1I ACTGG 3 cut(s) 371, 603, 634
BseBI CCWGG 1 cut(s) 385
BseDI CCNNGG 1 cut(s) 433
BseLI CCNNNNNNNGG 1 cut(s) 38
BseNI ACTGG 3 cut(s) 371, 603, 634
BseRI GAGGAG 2 cut(s) 28, 255
BseSI GKGCMC 1 cut(s) 490
BsiHKAI GWGCWC 1 cut(s) 490
BsiSI CCGG 1 cut(s) 8
BslI CCNNNNNNNGG 1 cut(s) 38
BsmAI GTCTC 3 cut(s) 70, 99, 261
BsmBI CGTCTC 2 cut(s) 70, 261
Bsp1286I GDGCHC 1 cut(s) 490
Bsp143I GATC 4 cut(s) 80, 194, 676, 693
BspACI CCGC 1 cut(s) 418
BspPI GGATC 1 cut(s) 202
BspQI GCTCTTC 1 cut(s) 347
BsrI ACTGG 3 cut(s) 371, 603, 634
BssECI CCNNGG 1 cut(s) 433
BssMI GATC 4 cut(s) 80, 194, 676, 693
BssNI GRCGYC 1 cut(s) 42
BssSI CACGAG 1 cut(s) 276
BssT1I CCWWGG 1 cut(s) 433
Bst2BI CACGAG 1 cut(s) 276
Bst2UI CCWGG 1 cut(s) 385
Bst4CI ACNGT 2 cut(s) 233, 707
Bst6I CTCTTC 2 cut(s) 259, 347
BstACI GRCGYC 1 cut(s) 42
BstC8I GCNNGC 1 cut(s) 655
BstDEI CTNAG 1 cut(s) 181
BstKTI GATC 4 cut(s) 83, 197, 679, 696
BstMAI GTCTC 3 cut(s) 70, 99, 261
BstMBI GATC 4 cut(s) 80, 194, 676, 693
BstMWI GCNNNNNNNGC 4 cut(s) 278, 415, 639, 654
BstNI CCWGG 1 cut(s) 385
BstSCI CCNGG 1 cut(s) 383
BstSLI GKGCMC 1 cut(s) 490
BstV2I GAAGAC 2 cut(s) 515, 672
BstX2I RGATCY 3 cut(s) 80, 194, 693
BstXI CCANNNNNNTGG 1 cut(s) 260
BstYI RGATCY 3 cut(s) 80, 194, 693
BtsI GCAGTG 1 cut(s) 349
BtsIMutI CAGTG 1 cut(s) 349
Cac8I GCNNGC 1 cut(s) 655
Cfr13I GGNCC 1 cut(s) 387
CseI GACGC 2 cut(s) 31, 278
Csp6I GTAC 4 cut(s) 156, 247, 421, 602
CviAII CATG 1 cut(s) 496
CviJI RGCY 7 cut(s) 409, 432, 464, 476, 521, 633, 687
CviKI_1 RGCY 7 cut(s) 409, 432, 464, 476, 521, 633, 687
CviQI GTAC 4 cut(s) 156, 247, 421, 602
DdeI CTNAG 1 cut(s) 181
DpnI GATC 4 cut(s) 82, 196, 678, 695
DpnII GATC 4 cut(s) 80, 194, 676, 693
Eam1104I CTCTTC 2 cut(s) 259, 347
EarI CTCTTC 2 cut(s) 259, 347
Eco130I CCWWGG 1 cut(s) 433
Eco47I GGWCC 1 cut(s) 387
Eco57I CTGAAG 4 cut(s) 61, 153, 486, 534
EcoO109I RGGNCCY 1 cut(s) 387
EcoRII CCWGG 1 cut(s) 383
EcoT14I CCWWGG 1 cut(s) 433
ErhI CCWWGG 1 cut(s) 433
Esp3I CGTCTC 2 cut(s) 70, 261
FaeI CATG 1 cut(s) 499
FaiI YATR 4 cut(s) 150, 176, 497, 699
FalI AAGNNNNNCTT 4 cut(s) 256, 288, 657, 689
FatI CATG 1 cut(s) 495
FbaI TGATCA 1 cut(s) 676
FspBI CTAG 1 cut(s) 339
GsuI CTGGAG 2 cut(s) 367, 463
HapII CCGG 1 cut(s) 8
HgaI GACGC 2 cut(s) 31, 278
Hin1I GRCGYC 1 cut(s) 42
Hin1II CATG 1 cut(s) 499
HincII GTYRAC 1 cut(s) 364
HindII GTYRAC 1 cut(s) 364
HindIII AAGCTT 1 cut(s) 407
HinfI GANTC 5 cut(s) 4, 187, 320, 439, 550
HpaII CCGG 1 cut(s) 8
HphI GGTGA 1 cut(s) 361
Hpy166II GTNNAC 3 cut(s) 364, 488, 710
Hpy188I TCNGA 1 cut(s) 80
Hpy188III TCNNGA 3 cut(s) 480, 543, 554
Hpy8I GTNNAC 3 cut(s) 364, 488, 710
Hpy99I CGWCG 1 cut(s) 59
HpyAV CCTTC 2 cut(s) 97, 554
HpyCH4III ACNGT 2 cut(s) 233, 707
HpyCH4IV ACGT 1 cut(s) 47
HpyCH4V TGCA 4 cut(s) 281, 488, 537, 729
HpyF10VI GCNNNNNNNGC 4 cut(s) 278, 415, 639, 654
HpyF3I CTNAG 1 cut(s) 181
HpySE526I ACGT 1 cut(s) 47
Hsp92I GRCGYC 1 cut(s) 42
Hsp92II CATG 1 cut(s) 499
Ksp22I TGATCA 1 cut(s) 676
Kzo9I GATC 4 cut(s) 80, 194, 676, 693
LguI GCTCTTC 1 cut(s) 347
LmnI GCTCC 1 cut(s) 461
MaeI CTAG 1 cut(s) 339
MaeII ACGT 1 cut(s) 47
MaeIII GTNAC 3 cut(s) 43, 208, 324
MalI GATC 4 cut(s) 82, 196, 678, 695
MboI GATC 4 cut(s) 80, 194, 676, 693
MboII GAAGA 7 cut(s) 75, 146, 276, 364, 520, 527, 677
MflI RGATCY 3 cut(s) 80, 194, 693
MhlI GDGCHC 1 cut(s) 490
MluCI AATT 3 cut(s) 137, 221, 532
MlyI GAGTC 1 cut(s) 559
MmeI TCCRAC 2 cut(s) 467, 565
MseI TTAA 1 cut(s) 456
MspI CCGG 1 cut(s) 8
MspR9I CCNGG 1 cut(s) 385
MvaI CCWGG 1 cut(s) 385
MwoI GCNNNNNNNGC 4 cut(s) 278, 415, 639, 654
NdeII GATC 4 cut(s) 80, 194, 676, 693
NlaIII CATG 1 cut(s) 499
NmuCI GTSAC 2 cut(s) 43, 208
PciSI GCTCTTC 1 cut(s) 347
PcsI WCGNNNNNNNCGW 1 cut(s) 60
PfeI GAWTC 4 cut(s) 4, 187, 320, 439
PfoI TCCNGGA 1 cut(s) 383
PleI GAGTC 1 cut(s) 558
PpsI GAGTC 1 cut(s) 558
PpuMI RGGWCCY 1 cut(s) 387
Psp5II RGGWCCY 1 cut(s) 387
Psp6I CCWGG 1 cut(s) 383
PspGI CCWGG 1 cut(s) 383
PspPI GGNCC 1 cut(s) 387
PspPPI RGGWCCY 1 cut(s) 387
PsuI RGATCY 3 cut(s) 80, 194, 693
RsaI GTAC 4 cut(s) 157, 248, 422, 603
RsaNI GTAC 4 cut(s) 156, 247, 421, 602
SapI GCTCTTC 1 cut(s) 347
SaqAI TTAA 1 cut(s) 456
Sau3AI GATC 4 cut(s) 80, 194, 676, 693
Sau96I GGNCC 1 cut(s) 387
SchI GAGTC 1 cut(s) 559
ScrFI CCNGG 1 cut(s) 385
SduI GDGCHC 1 cut(s) 490
SinI GGWCC 1 cut(s) 387
Sse9I AATT 3 cut(s) 137, 221, 532
SsiI CCGC 1 cut(s) 418
SspMI CTAG 1 cut(s) 339
StyD4I CCNGG 1 cut(s) 383
StyI CCWWGG 1 cut(s) 433
TaaI ACNGT 2 cut(s) 233, 707
TaiI ACGT 1 cut(s) 50
TaqI TCGA 3 cut(s) 204, 530, 635
TasI AATT 3 cut(s) 137, 221, 532
TfiI GAWTC 4 cut(s) 4, 187, 320, 439
Tru1I TTAA 1 cut(s) 456
Tru9I TTAA 1 cut(s) 456
TscAI CASTG 1 cut(s) 349
TseFI GTSAC 2 cut(s) 43, 208
Tsp45I GTSAC 2 cut(s) 43, 208
TspDTI ATGAA 3 cut(s) 177, 215, 573
TspRI CASTG 1 cut(s) 349
VneI GTGCAC 1 cut(s) 486
VpaK11BI GGWCC 1 cut(s) 387
XapI RAATTY 1 cut(s) 221
XspI CTAG 1 cut(s) 339
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.