Rw2G041960

Belongs to the cytochrome b5 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0013285
Physical Location & Seq
Reverse (-)
68196171 .. 68199473
3303 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G041960.1

Sequence Viewer

Length: 720 bp
ATGGCGGCAAAGATTTTAACGTCTCCTTTTGTAGGAATTGCCGTTTTTGTCACCTTGCTCGCTCTCTTCGCCTTTTACTATAAGCCACTCTCAAAACAGAGATTGTTCACTGTTGAAGAATTGGCTTTGTACAATGGGACTGATCAAAGTTTGCCAATCTTGTTAGGAATTCTCGGATCGGTTTTCGATGTGACAAAAGGAAAGTCTCATTACGGTGAGGGTGGGGGTTATAATCACTTTTCCGGAAGAGATGCTTCACGAGCATTTGTTTCTGGAAACTTTACAGGAGATGGGCTCACAGACTCATTAAGCGGTTTATCCAGCCAACAGGTGAAGAGTATTGTTGAATGGAGGGATTTCTACTTTAGAACTTACACATTTGTTGGTAAGCTAGTAGGTCGGTACTATGATCCCAAAGGAAATCCTACGAAATATTTGAAGGGAGTTGAAGCAAAGGCTGCCAGAGGTGCACAGCTATTGAAGAAACAGGAGATTGAAGAAGCTAAGCAACCTGGTTGCAATTCAAGGTGGAGTCAGGATGAAGGCGGAGAGGTTTGGTGCGATGTGGGCGTCCCACGATTAGTTCAGAGGCCACTAGAAATTGCTTTGACTGGGAAAATGAGCAAGCGATGTGCGTGCTTTAAAGAGGATCAACTGAGCCAACCGGGGTTGGAAGTCTACGAAGGATGTGATTATCTTGCCAAGACCTGCCGAGTCTAA

Protein Analysis

239

Amino Acids

26.43

Weight (kDa)

8.79

Isoelectric Point (pI)

42.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyt-b5 PF00173 37 - 97 1.9e-07 Cytochrome b5-like Heme/Steroid binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013285)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G14965 AT4G14965
fragaria_vesca FvH4_6g40100 FvH4_6g40100
malus_domestica MD09G1135300.v1.1
prunus_persica Prupe.3G191400_v2.0.a1
pyrus_communis pycom09g05640
rosa_chinensis RchiOBHm_Chr2g0154731
rosa_laevigata RLG00000020798
rosa_multiflora Rmu_sc0001004.1_g000005 Rmu_sc0001027.1_g000007
rosa_roxburghii Rroxscaffold_2G00094150
rosa_rugosa Rorug02G0444800
rosa_samantha Rh2AG510200 Rh2BG521100 Rh2CG495400 Rh2DG531200
rosa_wichuraiana Rw2G041960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 231
Acc36I ACCTGC 1 cut(s) 716
AccI GTMKAC 1 cut(s) 678
AccIII TCCGGA 1 cut(s) 242
AciI CCGC 3 cut(s) 5, 312, 546
AclWI GGATC 3 cut(s) 184, 404, 657
AcsI RAATTY 1 cut(s) 168
AcyI GRCGYC 1 cut(s) 570
AfaI GTAC 2 cut(s) 131, 404
AfiI CCNNNNNNNGG 1 cut(s) 32
AgsI TTSAA 7 cut(s) 116, 347, 439, 449, 481, 497, 525
AjnI CCWGG 1 cut(s) 511
AluBI AGCT 3 cut(s) 391, 475, 503
AluI AGCT 3 cut(s) 391, 475, 503
Alw21I GWGCWC 1 cut(s) 472
Alw26I GTCTC 2 cut(s) 27, 210
Alw44I GTGCAC 1 cut(s) 468
AlwI GGATC 3 cut(s) 184, 404, 657
Aor13HI TCCGGA 1 cut(s) 242
AoxI GGCC 1 cut(s) 590
ApaLI GTGCAC 1 cut(s) 468
ApeKI GCWGC 1 cut(s) 458
ApoI RAATTY 1 cut(s) 168
ArsI GACNNNNNNTTYG 2 cut(s) 188, 220
AsuC2I CCSGG 1 cut(s) 666
AsuHPI GGTGA 3 cut(s) 43, 227, 343
BaeGI GKGCMC 1 cut(s) 472
BanII GRGCYC 1 cut(s) 297
BauI CACGAG 1 cut(s) 258
Bbv12I GWGCWC 1 cut(s) 472
BbvI GCAGC 1 cut(s) 445
BccI CCATC 1 cut(s) 284
BceAI ACGGC 1 cut(s) 26
BcgI CGANNNNNNTGC 2 cut(s) 618, 652
BciT130I CCWGG 1 cut(s) 513
BclI TGATCA 1 cut(s) 142
BcnI CCSGG 1 cut(s) 666
BcoDI GTCTC 2 cut(s) 27, 210
BfaI CTAG 2 cut(s) 392, 596
BfuAI ACCTGC 1 cut(s) 716
BisI GCNGC 2 cut(s) 6, 459
BlpI GCTNAGC 1 cut(s) 504
BlsI GCNGC 2 cut(s) 7, 460
Bme1390I CCNGG 2 cut(s) 513, 666
BmrFI CCNGG 2 cut(s) 513, 666
BmrI ACTGGG 1 cut(s) 621
BmsI GCATC 1 cut(s) 241
BmuI ACTGGG 1 cut(s) 621
BplI GAGNNNNNCTC 2 cut(s) 279, 311
Bpu1102I GCTNAGC 1 cut(s) 504
BpuMI CCSGG 1 cut(s) 666
BsaHI GRCGYC 1 cut(s) 570
BsaJI CCNNGG 1 cut(s) 665
BsaWI WCCGGW 1 cut(s) 242
Bsc4I CCNNNNNNNGG 1 cut(s) 32
Bse1I ACTGG 1 cut(s) 616
BseAI TCCGGA 1 cut(s) 242
BseBI CCWGG 1 cut(s) 513
BseDI CCNNGG 1 cut(s) 665
BseGI GGATG 2 cut(s) 544, 692
BseLI CCNNNNNNNGG 1 cut(s) 32
BseMII CTCAG 1 cut(s) 647
BseNI ACTGG 1 cut(s) 616
BseSI GKGCMC 1 cut(s) 472
BseXI GCAGC 1 cut(s) 445
BshFI GGCC 1 cut(s) 592
BsiHKAI GWGCWC 1 cut(s) 472
BsiSI CCGG 2 cut(s) 243, 665
BslFI GGGAC 2 cut(s) 151, 557
BslI CCNNNNNNNGG 1 cut(s) 32
BsmAI GTCTC 2 cut(s) 27, 210
BsmBI CGTCTC 1 cut(s) 27
BsmFI GGGAC 2 cut(s) 151, 557
BsnI GGCC 1 cut(s) 592
Bsp1286I GDGCHC 2 cut(s) 297, 472
Bsp13I TCCGGA 1 cut(s) 242
Bsp1407I TGTACA 1 cut(s) 129
Bsp143I GATC 4 cut(s) 142, 176, 409, 649
Bsp1720I GCTNAGC 1 cut(s) 504
BspACI CCGC 3 cut(s) 5, 312, 546
BspANI GGCC 1 cut(s) 592
BspCNI CTCAG 1 cut(s) 648
BspEI TCCGGA 1 cut(s) 242
BspMI ACCTGC 1 cut(s) 716
BspPI GGATC 3 cut(s) 184, 404, 657
BsrGI TGTACA 1 cut(s) 129
BsrI ACTGG 1 cut(s) 616
BssECI CCNNGG 1 cut(s) 665
BssMI GATC 4 cut(s) 142, 176, 409, 649
BssNI GRCGYC 1 cut(s) 570
BssSI CACGAG 1 cut(s) 258
Bst2BI CACGAG 1 cut(s) 258
Bst2UI CCWGG 1 cut(s) 513
Bst4CI ACNGT 2 cut(s) 112, 215
Bst6I CTCTTC 3 cut(s) 71, 241, 329
BstACI GRCGYC 1 cut(s) 570
BstAPI GCANNNNNTGC 1 cut(s) 458
BstAUI TGTACA 1 cut(s) 129
BstC8I GCNNGC 3 cut(s) 60, 626, 637
BstDEI CTNAG 2 cut(s) 504, 656
BstENI CCTNNNNNAGG 1 cut(s) 30
BstF5I GGATG 2 cut(s) 544, 692
BstKTI GATC 4 cut(s) 145, 179, 412, 652
BstMAI GTCTC 2 cut(s) 27, 210
BstMBI GATC 4 cut(s) 142, 176, 409, 649
BstMWI GCNNNNNNNGC 5 cut(s) 68, 260, 458, 467, 567
BstNI CCWGG 1 cut(s) 513
BstSCI CCNGG 2 cut(s) 511, 664
BstSLI GKGCMC 1 cut(s) 472
BstV1I GCAGC 1 cut(s) 445
BsuRI GGCC 1 cut(s) 592
BtgZI GCGATG 2 cut(s) 576, 643
BtsCI GGATG 2 cut(s) 544, 692
BtsIMutI CAGTG 1 cut(s) 108
BveI ACCTGC 1 cut(s) 716
Cac8I GCNNGC 3 cut(s) 60, 626, 637
CseI GACGC 1 cut(s) 559
CsiI ACCWGGT 1 cut(s) 511
Csp6I GTAC 2 cut(s) 130, 403
CviQI GTAC 2 cut(s) 130, 403
DdeI CTNAG 2 cut(s) 504, 656
DpnI GATC 4 cut(s) 144, 178, 411, 651
DpnII GATC 4 cut(s) 142, 176, 409, 649
DraI TTTAAA 1 cut(s) 643
Eam1104I CTCTTC 3 cut(s) 71, 241, 329
EarI CTCTTC 3 cut(s) 71, 241, 329
EciI GGCGGA 1 cut(s) 561
Eco24I GRGCYC 1 cut(s) 297
EcoNI CCTNNNNNAGG 1 cut(s) 30
EcoRI GAATTC 1 cut(s) 168
EcoRII CCWGG 1 cut(s) 511
EcoT38I GRGCYC 1 cut(s) 297
Esp3I CGTCTC 1 cut(s) 27
FaiI YATR 3 cut(s) 81, 231, 408
FalI AAGNNNNNCTT 2 cut(s) 238, 270
FaqI GGGAC 2 cut(s) 151, 557
FbaI TGATCA 1 cut(s) 142
FblI GTMKAC 1 cut(s) 678
Fnu4HI GCNGC 2 cut(s) 6, 459
FokI GGATG 2 cut(s) 551, 699
FriOI GRGCYC 1 cut(s) 297
Fsp4HI GCNGC 2 cut(s) 6, 459
FspBI CTAG 2 cut(s) 392, 596
GluI GCNGC 2 cut(s) 6, 459
HaeIII GGCC 1 cut(s) 592
HapII CCGG 2 cut(s) 243, 665
HgaI GACGC 1 cut(s) 559
Hin1I GRCGYC 1 cut(s) 570
HinfI GANTC 3 cut(s) 302, 532, 714
HpaII CCGG 2 cut(s) 243, 665
HphI GGTGA 3 cut(s) 43, 227, 343
Hpy166II GTNNAC 3 cut(s) 108, 470, 679
Hpy188I TCNGA 2 cut(s) 176, 588
Hpy188III TCNNGA 4 cut(s) 243, 258, 273, 536
Hpy8I GTNNAC 3 cut(s) 108, 470, 679
HpyAV CCTTC 3 cut(s) 433, 536, 677
HpyCH4III ACNGT 2 cut(s) 112, 215
HpyCH4IV ACGT 1 cut(s) 20
HpyCH4V TGCA 2 cut(s) 470, 519
HpyF10VI GCNNNNNNNGC 5 cut(s) 68, 260, 458, 467, 567
HpyF3I CTNAG 2 cut(s) 504, 656
HpySE526I ACGT 1 cut(s) 20
Hsp92I GRCGYC 1 cut(s) 570
Kpn2I TCCGGA 1 cut(s) 242
Ksp22I TGATCA 1 cut(s) 142
Kzo9I GATC 4 cut(s) 142, 176, 409, 649
Lsp1109I GCAGC 1 cut(s) 445
LweI GCATC 1 cut(s) 241
MabI ACCWGGT 1 cut(s) 511
MaeI CTAG 2 cut(s) 392, 596
MaeII ACGT 1 cut(s) 20
MaeIII GTNAC 2 cut(s) 49, 190
MalI GATC 4 cut(s) 144, 178, 411, 651
MboI GATC 4 cut(s) 142, 176, 409, 649
MboII GAAGA 6 cut(s) 58, 128, 258, 346, 493, 509
MhlI GDGCHC 2 cut(s) 297, 472
MluCI AATT 5 cut(s) 36, 119, 168, 520, 600
MlyI GAGTC 2 cut(s) 296, 541
MmeI TCCRAC 1 cut(s) 651
MnlI CCTC 6 cut(s) 211, 345, 458, 544, 582, 640
MroI TCCGGA 1 cut(s) 242
MseI TTAA 3 cut(s) 17, 308, 642
MslI CAYNNNNRTG 1 cut(s) 213
MspI CCGG 2 cut(s) 243, 665
MspR9I CCNGG 2 cut(s) 513, 666
MvaI CCWGG 1 cut(s) 513
MwoI GCNNNNNNNGC 5 cut(s) 68, 260, 458, 467, 567
NciI CCSGG 1 cut(s) 666
NdeII GATC 4 cut(s) 142, 176, 409, 649
NmuCI GTSAC 2 cut(s) 49, 190
PkrI GCNGC 2 cut(s) 7, 460
PleI GAGTC 2 cut(s) 296, 540
PpsI GAGTC 2 cut(s) 296, 540
PsiI TTATAA 1 cut(s) 231
Psp6I CCWGG 1 cut(s) 511
PspGI CCWGG 1 cut(s) 511
RsaI GTAC 2 cut(s) 131, 404
RsaNI GTAC 2 cut(s) 130, 403
RseI CAYNNNNRTG 1 cut(s) 213
SaqAI TTAA 3 cut(s) 17, 308, 642
SatI GCNGC 2 cut(s) 6, 459
Sau3AI GATC 4 cut(s) 142, 176, 409, 649
SchI GAGTC 2 cut(s) 296, 541
ScrFI CCNGG 2 cut(s) 513, 666
SduI GDGCHC 2 cut(s) 297, 472
SexAI ACCWGGT 1 cut(s) 511
SfaNI GCATC 1 cut(s) 241
SmiMI CAYNNNNRTG 1 cut(s) 213
Sse9I AATT 5 cut(s) 36, 119, 168, 520, 600
SsiI CCGC 3 cut(s) 5, 312, 546
SspI AATATT 1 cut(s) 434
SspMI CTAG 2 cut(s) 392, 596
StyD4I CCNGG 2 cut(s) 511, 664
TaaI ACNGT 2 cut(s) 112, 215
TaiI ACGT 1 cut(s) 23
TaqI TCGA 1 cut(s) 186
TasI AATT 5 cut(s) 36, 119, 168, 520, 600
TatI WGTACW 1 cut(s) 129
TauI GCSGC 1 cut(s) 8
Tru1I TTAA 3 cut(s) 17, 308, 642
Tru9I TTAA 3 cut(s) 17, 308, 642
TscAI CASTG 1 cut(s) 115
TseFI GTSAC 2 cut(s) 49, 190
TseI GCWGC 1 cut(s) 458
Tsp45I GTSAC 2 cut(s) 49, 190
TspDTI ATGAA 1 cut(s) 555
TspRI CASTG 1 cut(s) 115
VneI GTGCAC 1 cut(s) 468
XagI CCTNNNNNAGG 1 cut(s) 30
XapI RAATTY 1 cut(s) 168
XmiI GTMKAC 1 cut(s) 678
XspI CTAG 2 cut(s) 392, 596
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.