RLG00000020798

Belongs to the cytochrome b5 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0013285
Physical Location & Seq
Reverse (-)
68890021 .. 68893263
3243 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020798

Sequence Viewer

Length: 654 bp
ATGGCGGCAAAGATTTTAACTTCTCCTTTTGTAGGAATTGCCCTTTTTGTCACCTTGCTCGCTCTCTTCGCCTTTTACTATAAGCCACTCTCAAAACAGAGATTGTTCACTGTTGAAGAATTGGCTTTGTACAATGGGACTGATCAAAGTTTGCCAATCTTGTTAGGAATTCTCGGATCGGTTTTCGATGTGACGAAAGGAAAGTCTCATTACGGTGAGGGTGGGGGTTATAATCATTTTTCTGGAAGAGATGCTTCACGAGCATTTGTTTCTGGAAACTTTACAGGAGATGGGCTCACAGACTCGTTAAGTGGTTTATCCAGCCAACAGATCCATTACCAAAGAATTGGCTTGTGGATAAGTCATTTTCTTTATTTCCGCTCGAATTGCCTCTGGCCTCTTCTTTGGAATAAACTAAAACAGGAGATTGAAGAAGCTAAGCAACCTGGTTGCAATTCAAGGTGGAGTCAGGATGAGGGCGGAGAGGTTTGGTGTGATGTGGGTGTCCCACGATTAGTTCAGAGGCCACTAGAAATTGCTTTGACTGGGAAAATGAGCAAGCGATGTGCGTGCTTTAAAGAGGATCAACTGAGCCAACCAGGGTTGGAAGTCTATGAAGGATGTGAATATCTTGCCAAGACCTGCCGAGTTTAA

Protein Analysis

218

Amino Acids

24.25

Weight (kDa)

7.57

Isoelectric Point (pI)

57.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyt-b5 PF00173 37 - 95 2.1e-07 Cytochrome b5-like Heme/Steroid binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013285)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G14965 AT4G14965
fragaria_vesca FvH4_6g40100 FvH4_6g40100
malus_domestica MD09G1135300.v1.1
prunus_persica Prupe.3G191400_v2.0.a1
pyrus_communis pycom09g05640
rosa_chinensis RchiOBHm_Chr2g0154731
rosa_laevigata RLG00000020798
rosa_multiflora Rmu_sc0001004.1_g000005 Rmu_sc0001027.1_g000007
rosa_roxburghii Rroxscaffold_2G00094150
rosa_rugosa Rorug02G0444800
rosa_samantha Rh2AG510200 Rh2BG521100 Rh2CG495400 Rh2DG531200
rosa_wichuraiana Rw2G041960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 231
Acc36I ACCTGC 1 cut(s) 650
AccBSI CCGCTC 1 cut(s) 381
AciI CCGC 3 cut(s) 5, 379, 480
AclWI GGATC 3 cut(s) 184, 325, 591
AcsI RAATTY 1 cut(s) 168
AfaI GTAC 1 cut(s) 131
AfiI CCNNNNNNNGG 1 cut(s) 32
AgsI TTSAA 3 cut(s) 116, 431, 459
AjnI CCWGG 2 cut(s) 445, 598
AluBI AGCT 1 cut(s) 437
AluI AGCT 1 cut(s) 437
Alw26I GTCTC 1 cut(s) 210
AlwI GGATC 3 cut(s) 184, 325, 591
AoxI GGCC 2 cut(s) 395, 524
ApoI RAATTY 1 cut(s) 168
ArsI GACNNNNNNTTYG 2 cut(s) 188, 220
AsuHPI GGTGA 2 cut(s) 43, 227
BanII GRGCYC 1 cut(s) 297
BauI CACGAG 1 cut(s) 258
BccI CCATC 1 cut(s) 284
BcgI CGANNNNNNTGC 2 cut(s) 552, 586
BciT130I CCWGG 2 cut(s) 447, 600
BclI TGATCA 1 cut(s) 142
BcoDI GTCTC 1 cut(s) 210
BfaI CTAG 1 cut(s) 530
BfuAI ACCTGC 1 cut(s) 650
BisI GCNGC 1 cut(s) 6
BlpI GCTNAGC 1 cut(s) 438
BlsI GCNGC 1 cut(s) 7
Bme1390I CCNGG 2 cut(s) 447, 600
BmrFI CCNGG 2 cut(s) 447, 600
BmrI ACTGGG 1 cut(s) 555
BmsI GCATC 1 cut(s) 241
BmuI ACTGGG 1 cut(s) 555
BplI GAGNNNNNCTC 2 cut(s) 279, 311
Bpu1102I GCTNAGC 1 cut(s) 438
BsaJI CCNNGG 1 cut(s) 599
Bsc4I CCNNNNNNNGG 1 cut(s) 32
Bse1I ACTGG 1 cut(s) 550
BseBI CCWGG 2 cut(s) 447, 600
BseDI CCNNGG 1 cut(s) 599
BseGI GGATG 2 cut(s) 478, 626
BseLI CCNNNNNNNGG 1 cut(s) 32
BseMII CTCAG 1 cut(s) 581
BseNI ACTGG 1 cut(s) 550
BshFI GGCC 2 cut(s) 397, 526
BslFI GGGAC 2 cut(s) 151, 491
BslI CCNNNNNNNGG 1 cut(s) 32
BsmAI GTCTC 1 cut(s) 210
BsmFI GGGAC 2 cut(s) 151, 491
BsnI GGCC 2 cut(s) 397, 526
Bsp1286I GDGCHC 1 cut(s) 297
Bsp1407I TGTACA 1 cut(s) 129
Bsp143I GATC 4 cut(s) 142, 176, 330, 583
Bsp1720I GCTNAGC 1 cut(s) 438
BspACI CCGC 3 cut(s) 5, 379, 480
BspANI GGCC 2 cut(s) 397, 526
BspCNI CTCAG 1 cut(s) 582
BspMI ACCTGC 1 cut(s) 650
BspPI GGATC 3 cut(s) 184, 325, 591
BsrBI CCGCTC 1 cut(s) 381
BsrGI TGTACA 1 cut(s) 129
BsrI ACTGG 1 cut(s) 550
BssECI CCNNGG 1 cut(s) 599
BssMI GATC 4 cut(s) 142, 176, 330, 583
BssSI CACGAG 1 cut(s) 258
Bst2BI CACGAG 1 cut(s) 258
Bst2UI CCWGG 2 cut(s) 447, 600
Bst4CI ACNGT 2 cut(s) 112, 215
Bst6I CTCTTC 3 cut(s) 71, 241, 405
BstAUI TGTACA 1 cut(s) 129
BstC8I GCNNGC 3 cut(s) 60, 560, 571
BstDEI CTNAG 2 cut(s) 438, 590
BstENI CCTNNNNNAGG 1 cut(s) 30
BstF5I GGATG 2 cut(s) 478, 626
BstKTI GATC 4 cut(s) 145, 179, 333, 586
BstMAI GTCTC 1 cut(s) 210
BstMBI GATC 4 cut(s) 142, 176, 330, 583
BstMWI GCNNNNNNNGC 3 cut(s) 68, 260, 387
BstNI CCWGG 2 cut(s) 447, 600
BstSCI CCNGG 2 cut(s) 445, 598
BstX2I RGATCY 1 cut(s) 330
BstXI CCANNNNNNTGG 1 cut(s) 347
BstYI RGATCY 1 cut(s) 330
BsuRI GGCC 2 cut(s) 397, 526
BtgZI GCGATG 1 cut(s) 577
BtsCI GGATG 2 cut(s) 478, 626
BtsIMutI CAGTG 1 cut(s) 108
BveI ACCTGC 1 cut(s) 650
Cac8I GCNNGC 3 cut(s) 60, 560, 571
CsiI ACCWGGT 1 cut(s) 445
Csp6I GTAC 1 cut(s) 130
CviJI RGCY 9 cut(s) 85, 125, 295, 324, 351, 397, 437, 526, 594
CviKI_1 RGCY 9 cut(s) 85, 125, 295, 324, 351, 397, 437, 526, 594
CviQI GTAC 1 cut(s) 130
DdeI CTNAG 2 cut(s) 438, 590
DpnI GATC 4 cut(s) 144, 178, 332, 585
DpnII GATC 4 cut(s) 142, 176, 330, 583
DraI TTTAAA 1 cut(s) 577
Eam1104I CTCTTC 3 cut(s) 71, 241, 405
EarI CTCTTC 3 cut(s) 71, 241, 405
EciI GGCGGA 1 cut(s) 495
Eco24I GRGCYC 1 cut(s) 297
EcoNI CCTNNNNNAGG 1 cut(s) 30
EcoRI GAATTC 1 cut(s) 168
EcoRII CCWGG 2 cut(s) 445, 598
EcoT38I GRGCYC 1 cut(s) 297
FaiI YATR 3 cut(s) 81, 231, 615
FalI AAGNNNNNCTT 2 cut(s) 238, 270
FaqI GGGAC 2 cut(s) 151, 491
FbaI TGATCA 1 cut(s) 142
Fnu4HI GCNGC 1 cut(s) 6
FokI GGATG 2 cut(s) 485, 633
FriOI GRGCYC 1 cut(s) 297
Fsp4HI GCNGC 1 cut(s) 6
FspBI CTAG 1 cut(s) 530
GluI GCNGC 1 cut(s) 6
HaeIII GGCC 2 cut(s) 397, 526
HinfI GANTC 2 cut(s) 302, 466
HphI GGTGA 2 cut(s) 43, 227
Hpy166II GTNNAC 1 cut(s) 108
Hpy188I TCNGA 2 cut(s) 176, 522
Hpy188III TCNNGA 4 cut(s) 243, 258, 273, 470
Hpy8I GTNNAC 1 cut(s) 108
HpyAV CCTTC 1 cut(s) 611
HpyCH4III ACNGT 2 cut(s) 112, 215
HpyCH4V TGCA 1 cut(s) 453
HpyF10VI GCNNNNNNNGC 3 cut(s) 68, 260, 387
HpyF3I CTNAG 2 cut(s) 438, 590
Ksp22I TGATCA 1 cut(s) 142
Kzo9I GATC 4 cut(s) 142, 176, 330, 583
LweI GCATC 1 cut(s) 241
MabI ACCWGGT 1 cut(s) 445
MaeI CTAG 1 cut(s) 530
MaeIII GTNAC 2 cut(s) 49, 190
MalI GATC 4 cut(s) 144, 178, 332, 585
MbiI CCGCTC 1 cut(s) 381
MboI GATC 4 cut(s) 142, 176, 330, 583
MboII GAAGA 5 cut(s) 58, 128, 258, 392, 443
MflI RGATCY 1 cut(s) 330
MhlI GDGCHC 1 cut(s) 297
MluCI AATT 7 cut(s) 36, 119, 168, 345, 385, 454, 534
MlyI GAGTC 2 cut(s) 296, 475
MmeI TCCRAC 1 cut(s) 585
MnlI CCTC 7 cut(s) 211, 401, 408, 469, 478, 516, 574
MseI TTAA 4 cut(s) 17, 308, 576, 652
MslI CAYNNNNRTG 1 cut(s) 213
MspR9I CCNGG 2 cut(s) 447, 600
MvaI CCWGG 2 cut(s) 447, 600
MwoI GCNNNNNNNGC 3 cut(s) 68, 260, 387
NdeII GATC 4 cut(s) 142, 176, 330, 583
NmuCI GTSAC 2 cut(s) 49, 190
PkrI GCNGC 1 cut(s) 7
PleI GAGTC 2 cut(s) 296, 474
PpsI GAGTC 2 cut(s) 296, 474
PsiI TTATAA 1 cut(s) 231
Psp6I CCWGG 2 cut(s) 445, 598
PspGI CCWGG 2 cut(s) 445, 598
PsuI RGATCY 1 cut(s) 330
RsaI GTAC 1 cut(s) 131
RsaNI GTAC 1 cut(s) 130
RseI CAYNNNNRTG 1 cut(s) 213
SaqAI TTAA 4 cut(s) 17, 308, 576, 652
SatI GCNGC 1 cut(s) 6
Sau3AI GATC 4 cut(s) 142, 176, 330, 583
SchI GAGTC 2 cut(s) 296, 475
ScrFI CCNGG 2 cut(s) 447, 600
SduI GDGCHC 1 cut(s) 297
SetI ASST 6 cut(s) 56, 439, 448, 464, 489, 644
SexAI ACCWGGT 1 cut(s) 445
SfaNI GCATC 1 cut(s) 241
SmiMI CAYNNNNRTG 1 cut(s) 213
Sse9I AATT 7 cut(s) 36, 119, 168, 345, 385, 454, 534
SsiI CCGC 3 cut(s) 5, 379, 480
SspMI CTAG 1 cut(s) 530
StyD4I CCNGG 2 cut(s) 445, 598
TaaI ACNGT 2 cut(s) 112, 215
TaqI TCGA 2 cut(s) 186, 383
TasI AATT 7 cut(s) 36, 119, 168, 345, 385, 454, 534
TatI WGTACW 1 cut(s) 129
TauI GCSGC 1 cut(s) 8
Tru1I TTAA 4 cut(s) 17, 308, 576, 652
Tru9I TTAA 4 cut(s) 17, 308, 576, 652
TscAI CASTG 1 cut(s) 115
TseFI GTSAC 2 cut(s) 49, 190
Tsp45I GTSAC 2 cut(s) 49, 190
TspDTI ATGAA 1 cut(s) 630
TspRI CASTG 1 cut(s) 115
XagI CCTNNNNNAGG 1 cut(s) 30
XapI RAATTY 1 cut(s) 168
XspI CTAG 1 cut(s) 530
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.