Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0013285

Physical Location & Seq

Genomic Coordinates Forward (+)

10390955 .. 10394361

Gene Structure 3407 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1135300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 723 bp

ATGGCGCCAAAGCTCTTAGCAACTTCCCCGTTTGTAGGAATTGCAGTACTCGTCCCCCTCATAGCTCTCTTCGCCTTCTATTACAAGCCCTTCTCCCAACAGAGATTGTTCACTGTTGAAGAACTGGCTTTGTACAACGGGACTGATGTGAGTTTACCGATTTTCCTAGGAATTCTCGGGTCAGTTTTCGATGTGACGAAAGGAAAATCTCATTATGGTGAGGGTGGGGGGTACAATCATTTTTCTGGAAGAGATGCTTCACGAGCATTTGTTTCAGGAAACTTTACAGGAGATGGCCTCACAGACTCGTTACGTGGTTTATCAAGTACACAGGTGAAGAGTGTCGTTGAATGGAGGGATTTCTACTTTAAAAGTTACACATTTGTAGGGAAGCTAGTAGGTCGTTACTATGATTCTCAAGGAAATCCTACAAAATATTTGAAGGGAGTGGAAGCAAAGGCTGCGAGAGGTGCTCAGCTTTTGGAGAAACAGGAGAATGAAGAAGCGAAGCAACCTGGTTGCAACTCAAGGTGGAGTCAGGATGAGGGTGGAGAGGTTTGGTGCGATGAGGGTGTCCCAAGGTTAGTTCAGAGACCGCTACAAATGGCTTTGACTGGGAAAATGAGCACACGATGCGCATGCTTTAATGAAGATCAACTGGACCGACCGGGGTTAAAGATCTACGAAGGATGTGATTATCTTGCCAAGACCTGCCGAGTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005575 GO:0005576 GO:0005623 GO:0007275 GO:0007399 GO:0008150 GO:0009987 GO:0010720 GO:0012505 GO:0016020 GO:0020037 GO:0022008 GO:0030154 GO:0032501 GO:0032502 GO:0044464 GO:0045595 GO:0045597 GO:0045664 GO:0045666 GO:0046906 GO:0048037 GO:0048518 GO:0048522 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0050767 GO:0050769 GO:0050789 GO:0050793 GO:0050794 GO:0051094 GO:0051239 GO:0051240 GO:0051960 GO:0051962 GO:0060284 GO:0065007 GO:0097159 GO:1901363 GO:2000026

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cyt-b5

Protein Analysis

241

Amino Acids

26.58

Weight (kDa)

8.14

Isoelectric Point (pI)

39.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cyt-b5	PF00173	38 - 98	2.5e-07	Cytochrome b5-like Heme/Steroid binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013285)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G14965 AT4G14965
fragaria_vesca	FvH4_6g40100 FvH4_6g40100
malus_domestica	MD09G1135300.v1.1
prunus_persica	Prupe.3G191400_v2.0.a1
pyrus_communis	pycom09g05640
rosa_chinensis	RchiOBHm_Chr2g0154731
rosa_laevigata	RLG00000020798
rosa_multiflora	Rmu_sc0001004.1_g000005 Rmu_sc0001027.1_g000007
rosa_roxburghii	Rroxscaffold_2G00094150
rosa_rugosa	Rorug02G0444800
rosa_samantha	Rh2AG510200 Rh2BG521100 Rh2CG495400 Rh2DG531200
rosa_wichuraiana	Rw2G041960

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	637
Acc36I	ACCTGC	1 cut(s)	719
AccB1I	GGYRCC	1 cut(s)	4
AciI	CCGC	1 cut(s)	596
AcsI	RAATTY	1 cut(s)	171
AcyI	GRCGYC	1 cut(s)	5
AfaI	GTAC	4 cut(s)	48, 134, 233, 328
AfiI	CCNNNNNNNGG	1 cut(s)	35
AgsI	TTSAA	3 cut(s)	119, 350, 442
AjnI	CCWGG	1 cut(s)	514
AluBI	AGCT	4 cut(s)	13, 65, 394, 478
AluI	AGCT	4 cut(s)	13, 65, 394, 478
Alw21I	GWGCWC	2 cut(s)	475, 629
Alw26I	GTCTC	1 cut(s)	586
Ama87I	CYCGRG	1 cut(s)	176
AoxI	GGCC	1 cut(s)	295
ApeKI	GCWGC	1 cut(s)	461
ApoI	RAATTY	1 cut(s)	171
AspA2I	CCTAGG	1 cut(s)	166
AspLEI	GCGC	2 cut(s)	7, 638
AspS9I	GGNCC	1 cut(s)	661
AsuC2I	CCSGG	1 cut(s)	669
AsuHPI	GGTGA	2 cut(s)	230, 346
AvaI	CYCGRG	1 cut(s)	176
AvaII	GGWCC	1 cut(s)	661
AvrII	CCTAGG	1 cut(s)	166
BanI	GGYRCC	1 cut(s)	4
BauI	CACGAG	1 cut(s)	261
Bbv12I	GWGCWC	2 cut(s)	475, 629
BbvI	GCAGC	1 cut(s)	448
BccI	CCATC	1 cut(s)	287
BcgI	CGANNNNNNTGC	2 cut(s)	621, 655
BciT130I	CCWGG	1 cut(s)	516
BcnI	CCSGG	1 cut(s)	669
BcoDI	GTCTC	1 cut(s)	586
BfaI	CTAG	2 cut(s)	167, 395
BfoI	RGCGCY	1 cut(s)	8
BfuAI	ACCTGC	1 cut(s)	719
BglII	AGATCT	1 cut(s)	678
BisI	GCNGC	1 cut(s)	462
BlnI	CCTAGG	1 cut(s)	166
BlpI	GCTNAGC	1 cut(s)	474
BlsI	GCNGC	1 cut(s)	463
BmcAI	AGTACT	1 cut(s)	48
Bme1390I	CCNGG	2 cut(s)	516, 669
Bme18I	GGWCC	1 cut(s)	661
BmeT110I	CYCGRG	1 cut(s)	176
BmgT120I	GGNCC	1 cut(s)	661
BmiI	GGNNCC	1 cut(s)	6
BmrFI	CCNGG	2 cut(s)	516, 669
BmrI	ACTGGG	1 cut(s)	624
BmsI	GCATC	2 cut(s)	244, 623
BmuI	ACTGGG	1 cut(s)	624
BplI	GAGNNNNNCTC	4 cut(s)	282, 314, 457, 489
Bpu1102I	GCTNAGC	1 cut(s)	474
BpuEI	CTTGAG	2 cut(s)	402, 511
BpuMI	CCSGG	1 cut(s)	669
BsaAI	YACGTR	1 cut(s)	314
BsaHI	GRCGYC	1 cut(s)	5
BsaI	GGTCTC	1 cut(s)	586
BsaJI	CCNNGG	3 cut(s)	166, 578, 668
Bsc4I	CCNNNNNNNGG	1 cut(s)	35
Bse1I	ACTGG	3 cut(s)	129, 619, 663
BseBI	CCWGG	1 cut(s)	516
BseDI	CCNNGG	3 cut(s)	166, 578, 668
BseGI	GGATG	2 cut(s)	547, 695
BseLI	CCNNNNNNNGG	1 cut(s)	35
BseMII	CTCAG	1 cut(s)	488
BseNI	ACTGG	3 cut(s)	129, 619, 663
BseXI	GCAGC	1 cut(s)	448
Bsh1285I	CGRYCG	1 cut(s)	668
BshFI	GGCC	1 cut(s)	297
BshNI	GGYRCC	1 cut(s)	4
BsiEI	CGRYCG	1 cut(s)	668
BsiHKAI	GWGCWC	2 cut(s)	475, 629
BsiHKCI	CYCGRG	1 cut(s)	176
BsiSI	CCGG	1 cut(s)	668
BslFI	GGGAC	3 cut(s)	38, 154, 560
BslI	CCNNNNNNNGG	1 cut(s)	35
BsmAI	GTCTC	1 cut(s)	586
BsmFI	GGGAC	3 cut(s)	38, 154, 560
BsnI	GGCC	1 cut(s)	297
Bso31I	GGTCTC	1 cut(s)	586
BsoBI	CYCGRG	1 cut(s)	176
Bsp1286I	GDGCHC	2 cut(s)	475, 629
Bsp1407I	TGTACA	1 cut(s)	132
Bsp143I	GATC	2 cut(s)	652, 678
Bsp1720I	GCTNAGC	1 cut(s)	474
BspACI	CCGC	1 cut(s)	596
BspANI	GGCC	1 cut(s)	297
BspCNI	CTCAG	1 cut(s)	487
BspLI	GGNNCC	1 cut(s)	6
BspMI	ACCTGC	1 cut(s)	719
BspT107I	GGYRCC	1 cut(s)	4
BspTNI	GGTCTC	1 cut(s)	586
BsrGI	TGTACA	1 cut(s)	132
BsrI	ACTGG	3 cut(s)	129, 619, 663
BssECI	CCNNGG	3 cut(s)	166, 578, 668
BssMI	GATC	2 cut(s)	652, 678
BssNI	GRCGYC	1 cut(s)	5
BssSI	CACGAG	1 cut(s)	261
BssT1I	CCWWGG	2 cut(s)	166, 578
Bst2BI	CACGAG	1 cut(s)	261
Bst2UI	CCWGG	1 cut(s)	516
Bst4CI	ACNGT	1 cut(s)	115
Bst6I	CTCTTC	3 cut(s)	74, 244, 332
BstACI	GRCGYC	1 cut(s)	5
BstAPI	GCANNNNNTGC	2 cut(s)	461, 633
BstAUI	TGTACA	1 cut(s)	132
BstBAI	YACGTR	1 cut(s)	314
BstC8I	GCNNGC	1 cut(s)	640
BstDEI	CTNAG	2 cut(s)	16, 474
BstF5I	GGATG	2 cut(s)	547, 695
BstH2I	RGCGCY	1 cut(s)	8
BstHHI	GCGC	2 cut(s)	7, 638
BstKTI	GATC	2 cut(s)	655, 681
BstMAI	GTCTC	1 cut(s)	586
BstMBI	GATC	2 cut(s)	652, 678
BstMCI	CGRYCG	1 cut(s)	668
BstMWI	GCNNNNNNNGC	5 cut(s)	71, 263, 461, 470, 633
BstNI	CCWGG	1 cut(s)	516
BstNSI	RCATGY	1 cut(s)	642
BstSCI	CCNGG	2 cut(s)	514, 667
BstV1I	GCAGC	1 cut(s)	448
BstX2I	RGATCY	1 cut(s)	678
BstYI	RGATCY	1 cut(s)	678
BsuRI	GGCC	1 cut(s)	297
BtgZI	GCGATG	1 cut(s)	579
BtsCI	GGATG	2 cut(s)	547, 695
BtsIMutI	CAGTG	1 cut(s)	111
BveI	ACCTGC	1 cut(s)	719
Cac8I	GCNNGC	1 cut(s)	640
CfoI	GCGC	2 cut(s)	7, 638
Cfr13I	GGNCC	1 cut(s)	661
CsiI	ACCWGGT	1 cut(s)	514
Csp6I	GTAC	4 cut(s)	47, 133, 232, 327
CviAII	CATG	1 cut(s)	639
CviJI	RGCY	9 cut(s)	13, 65, 88, 128, 297, 394, 461, 478, 608
CviKI_1	RGCY	9 cut(s)	13, 65, 88, 128, 297, 394, 461, 478, 608
CviQI	GTAC	4 cut(s)	47, 133, 232, 327
DdeI	CTNAG	2 cut(s)	16, 474
DinI	GGCGCC	1 cut(s)	6
DpnI	GATC	2 cut(s)	654, 680
DpnII	GATC	2 cut(s)	652, 678
DraI	TTTAAA	1 cut(s)	370
Eam1104I	CTCTTC	3 cut(s)	74, 244, 332
EarI	CTCTTC	3 cut(s)	74, 244, 332
Eco130I	CCWWGG	2 cut(s)	166, 578
Eco31I	GGTCTC	1 cut(s)	586
Eco47I	GGWCC	1 cut(s)	661
Eco88I	CYCGRG	1 cut(s)	176
EcoRI	GAATTC	1 cut(s)	171
EcoRII	CCWGG	1 cut(s)	514
EcoT14I	CCWWGG	2 cut(s)	166, 578
EgeI	GGCGCC	1 cut(s)	6
EheI	GGCGCC	1 cut(s)	6
ErhI	CCWWGG	2 cut(s)	166, 578
FaeI	CATG	1 cut(s)	642
FaiI	YATR	5 cut(s)	62, 216, 411, 640, 721
FalI	AAGNNNNNCTT	2 cut(s)	241, 273
FaqI	GGGAC	3 cut(s)	38, 154, 560
FatI	CATG	1 cut(s)	638
Fnu4HI	GCNGC	1 cut(s)	462
FokI	GGATG	2 cut(s)	554, 702
Fsp4HI	GCNGC	1 cut(s)	462
FspAI	RTGCGCAY	1 cut(s)	637
FspBI	CTAG	2 cut(s)	167, 395
FspI	TGCGCA	1 cut(s)	637
GlaI	GCGC	2 cut(s)	6, 637
GluI	GCNGC	1 cut(s)	462
HaeII	RGCGCY	1 cut(s)	8
HaeIII	GGCC	1 cut(s)	297
HapII	CCGG	1 cut(s)	668
HhaI	GCGC	2 cut(s)	7, 638
Hin1I	GRCGYC	1 cut(s)	5
Hin1II	CATG	1 cut(s)	642
Hin6I	GCGC	2 cut(s)	5, 636
HinP1I	GCGC	2 cut(s)	5, 636
HinfI	GANTC	3 cut(s)	305, 413, 535
HpaII	CCGG	1 cut(s)	668
HphI	GGTGA	2 cut(s)	230, 346
Hpy166II	GTNNAC	3 cut(s)	111, 155, 329
Hpy188I	TCNGA	1 cut(s)	591
Hpy188III	TCNNGA	4 cut(s)	246, 261, 276, 539
Hpy8I	GTNNAC	3 cut(s)	111, 155, 329
HpyAV	CCTTC	4 cut(s)	85, 100, 436, 680
HpyCH4III	ACNGT	1 cut(s)	115
HpyCH4IV	ACGT	1 cut(s)	313
HpyCH4V	TGCA	2 cut(s)	44, 522
HpyF10VI	GCNNNNNNNGC	5 cut(s)	71, 263, 461, 470, 633
HpyF3I	CTNAG	2 cut(s)	16, 474
HpySE526I	ACGT	1 cut(s)	313
Hsp92I	GRCGYC	1 cut(s)	5
Hsp92II	CATG	1 cut(s)	642
HspAI	GCGC	2 cut(s)	5, 636
KasI	GGCGCC	1 cut(s)	4
Kzo9I	GATC	2 cut(s)	652, 678
Lsp1109I	GCAGC	1 cut(s)	448
LweI	GCATC	2 cut(s)	244, 623
MabI	ACCWGGT	1 cut(s)	514
MaeI	CTAG	2 cut(s)	167, 395
MaeII	ACGT	1 cut(s)	313
MaeIII	GTNAC	4 cut(s)	193, 309, 374, 404
MalI	GATC	2 cut(s)	654, 680
MboI	GATC	2 cut(s)	652, 678
MboII	GAAGA	6 cut(s)	61, 131, 261, 349, 512, 662
MflI	RGATCY	1 cut(s)	678
MhlI	GDGCHC	2 cut(s)	475, 629
MluCI	AATT	2 cut(s)	39, 171
Mly113I	GGCGCC	1 cut(s)	5
MlyI	GAGTC	2 cut(s)	299, 544
MnlI	CCTC	8 cut(s)	68, 214, 308, 348, 461, 538, 547, 562
MseI	TTAA	3 cut(s)	369, 645, 674
MslI	CAYNNNNRTG	1 cut(s)	216
MspI	CCGG	1 cut(s)	668
MspR9I	CCNGG	2 cut(s)	516, 669
MvaI	CCWGG	1 cut(s)	516
MwoI	GCNNNNNNNGC	5 cut(s)	71, 263, 461, 470, 633
NarI	GGCGCC	1 cut(s)	5
NciI	CCSGG	1 cut(s)	669
NdeII	GATC	2 cut(s)	652, 678
NlaIII	CATG	1 cut(s)	642
NlaIV	GGNNCC	1 cut(s)	6
NmuCI	GTSAC	1 cut(s)	193
NsbI	TGCGCA	1 cut(s)	637
NspI	RCATGY	1 cut(s)	642
PaeI	GCATGC	1 cut(s)	642
PfeI	GAWTC	1 cut(s)	413
PkrI	GCNGC	1 cut(s)	463
PleI	GAGTC	2 cut(s)	299, 543
PluTI	GGCGCC	1 cut(s)	8
PpsI	GAGTC	2 cut(s)	299, 543
Ppu21I	YACGTR	1 cut(s)	314
Psp6I	CCWGG	1 cut(s)	514
PspGI	CCWGG	1 cut(s)	514
PspN4I	GGNNCC	1 cut(s)	6
PspPI	GGNCC	1 cut(s)	661
PsuI	RGATCY	1 cut(s)	678
RsaI	GTAC	4 cut(s)	48, 134, 233, 328
RsaNI	GTAC	4 cut(s)	47, 133, 232, 327
RseI	CAYNNNNRTG	1 cut(s)	216
SaqAI	TTAA	3 cut(s)	369, 645, 674
SatI	GCNGC	1 cut(s)	462
Sau3AI	GATC	2 cut(s)	652, 678
Sau96I	GGNCC	1 cut(s)	661
ScaI	AGTACT	1 cut(s)	48
SchI	GAGTC	2 cut(s)	299, 544
ScrFI	CCNGG	2 cut(s)	516, 669
SduI	GDGCHC	2 cut(s)	475, 629
SexAI	ACCWGGT	1 cut(s)	514
SfaNI	GCATC	2 cut(s)	244, 623
SfoI	GGCGCC	1 cut(s)	6
SinI	GGWCC	1 cut(s)	661
SmiMI	CAYNNNNRTG	1 cut(s)	216
SmlI	CTYRAG	2 cut(s)	417, 526
SmoI	CTYRAG	2 cut(s)	417, 526
SphI	GCATGC	1 cut(s)	642
Sse9I	AATT	2 cut(s)	39, 171
SsiI	CCGC	1 cut(s)	596
SspDI	GGCGCC	1 cut(s)	4
SspI	AATATT	1 cut(s)	437
SspMI	CTAG	2 cut(s)	167, 395
StyD4I	CCNGG	2 cut(s)	514, 667
StyI	CCWWGG	2 cut(s)	166, 578
TaaI	ACNGT	1 cut(s)	115
TaiI	ACGT	1 cut(s)	316
TaqI	TCGA	1 cut(s)	189
TaqII	GACCGA	1 cut(s)	678
TasI	AATT	2 cut(s)	39, 171
TatI	WGTACW	3 cut(s)	46, 132, 326
TfiI	GAWTC	1 cut(s)	413
Tru1I	TTAA	3 cut(s)	369, 645, 674
Tru9I	TTAA	3 cut(s)	369, 645, 674
TscAI	CASTG	1 cut(s)	118
TseFI	GTSAC	1 cut(s)	193
TseI	GCWGC	1 cut(s)	461
Tsp45I	GTSAC	1 cut(s)	193
TspDTI	ATGAA	2 cut(s)	513, 663
TspRI	CASTG	1 cut(s)	118
VpaK11BI	GGWCC	1 cut(s)	661
XapI	RAATTY	1 cut(s)	171
XceI	RCATGY	1 cut(s)	642
XmaJI	CCTAGG	1 cut(s)	166
XspI	CTAG	2 cut(s)	167, 395
ZrmI	AGTACT	1 cut(s)	48

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.