Rh2BG521100

Belongs to the cytochrome b5 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0013285
Physical Location & Seq
Reverse (-)
73179480 .. 73183161
3682 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG521100.1

Sequence Viewer

Length: 651 bp
ATGGCGGCAAAGATTTTAACGTCTCCTTTTGTAGGAATTGCCGTTTTTGTCACCTTGCTCGCTCTCTTCGCCTTTTACTATAAGCCACTCTCAAAACAGAGATTGTTCACTGTTGAAGAATTGGCTTTGTACAATGGGACTGATCAAAGTTTGCCAATCTTCTTAGGAATTCTCGGATCGGTTTTCGATGTGACGAAGGGAAAGTCTCATTACGGTGAGGGTGGGGGTTATAATCATTTTTCTGGAAGAGATGCTTCACGAGCATTTGTTTCTGGAAACTTTACAGGAGATGGGCTCACAGACTCGTTAAGCGGTTTATCCAGCCAACAGGTGAAGAGTATTGTTGAATGGAGGGATTTCTACTTTAGAACTTACACATTTGTTGGTAAGCTAGTAGGTCGGTACTATGATCCCAAAGGAAATCCTACTAAATATTTGAAGGGGGTTGAAGCAAAGGCTGCCAGAGGGGCACAGCTATTGAAGAAACAGGAGATTGAAGAAGCTAAGCAACCTGGTTGCAATTCAAGGTGGAGTCAGGATGAGGGCGGAGAGGTATGTGTTTTGGGTCTTATAATATTTCTGTGCCATGTGTATCAACTTTGTGCTGTTGTGGAGGCTTATAAACTAGGATACCAACTCAATGGTGTGTAG

Protein Analysis

216

Amino Acids

23.8

Weight (kDa)

8.77

Isoelectric Point (pI)

33.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyt-b5 PF00173 37 - 97 1.1e-07 Cytochrome b5-like Heme/Steroid binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013285)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G14965 AT4G14965
fragaria_vesca FvH4_6g40100 FvH4_6g40100
malus_domestica MD09G1135300.v1.1
prunus_persica Prupe.3G191400_v2.0.a1
pyrus_communis pycom09g05640
rosa_chinensis RchiOBHm_Chr2g0154731
rosa_laevigata RLG00000020798
rosa_multiflora Rmu_sc0001004.1_g000005 Rmu_sc0001027.1_g000007
rosa_roxburghii Rroxscaffold_2G00094150
rosa_rugosa Rorug02G0444800
rosa_samantha Rh2AG510200 Rh2BG521100 Rh2CG495400 Rh2DG531200
rosa_wichuraiana Rw2G041960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 231, 572, 621
AciI CCGC 3 cut(s) 5, 312, 546
AclWI GGATC 2 cut(s) 184, 404
AcsI RAATTY 1 cut(s) 168
AfaI GTAC 2 cut(s) 131, 404
AfiI CCNNNNNNNGG 1 cut(s) 32
AgsI TTSAA 7 cut(s) 116, 347, 439, 449, 481, 497, 525
AjnI CCWGG 1 cut(s) 511
AluBI AGCT 3 cut(s) 391, 475, 503
AluI AGCT 3 cut(s) 391, 475, 503
Alw26I GTCTC 2 cut(s) 27, 210
AlwI GGATC 2 cut(s) 184, 404
ApeKI GCWGC 1 cut(s) 458
ApoI RAATTY 1 cut(s) 168
ArsI GACNNNNNNTTYG 2 cut(s) 188, 220
AsuHPI GGTGA 3 cut(s) 43, 227, 343
BaeGI GKGCMC 1 cut(s) 472
BanII GRGCYC 1 cut(s) 297
BauI CACGAG 1 cut(s) 258
BbvI GCAGC 1 cut(s) 445
BccI CCATC 1 cut(s) 284
BceAI ACGGC 1 cut(s) 26
BciT130I CCWGG 1 cut(s) 513
BciVI GTATCC 1 cut(s) 623
BclI TGATCA 1 cut(s) 142
BcoDI GTCTC 2 cut(s) 27, 210
BfaI CTAG 2 cut(s) 392, 626
BfuI GTATCC 1 cut(s) 623
BglI GCCNNNNNGGC 1 cut(s) 467
BisI GCNGC 2 cut(s) 6, 459
BlpI GCTNAGC 1 cut(s) 504
BlsI GCNGC 2 cut(s) 7, 460
Bme1390I CCNGG 1 cut(s) 513
BmrFI CCNGG 1 cut(s) 513
BmsI GCATC 1 cut(s) 241
BplI GAGNNNNNCTC 2 cut(s) 279, 311
Bpu1102I GCTNAGC 1 cut(s) 504
Bsc4I CCNNNNNNNGG 1 cut(s) 32
BseBI CCWGG 1 cut(s) 513
BseGI GGATG 1 cut(s) 544
BseLI CCNNNNNNNGG 1 cut(s) 32
BseSI GKGCMC 1 cut(s) 472
BseXI GCAGC 1 cut(s) 445
BslFI GGGAC 1 cut(s) 151
BslI CCNNNNNNNGG 1 cut(s) 32
BsmAI GTCTC 2 cut(s) 27, 210
BsmBI CGTCTC 1 cut(s) 27
BsmFI GGGAC 1 cut(s) 151
Bsp1286I GDGCHC 2 cut(s) 297, 472
Bsp1407I TGTACA 1 cut(s) 129
Bsp143I GATC 3 cut(s) 142, 176, 409
Bsp1720I GCTNAGC 1 cut(s) 504
BspACI CCGC 3 cut(s) 5, 312, 546
BspPI GGATC 2 cut(s) 184, 404
BsrGI TGTACA 1 cut(s) 129
BssMI GATC 3 cut(s) 142, 176, 409
BssSI CACGAG 1 cut(s) 258
Bst2BI CACGAG 1 cut(s) 258
Bst2UI CCWGG 1 cut(s) 513
Bst4CI ACNGT 2 cut(s) 112, 215
Bst6I CTCTTC 3 cut(s) 71, 241, 329
BstAPI GCANNNNNTGC 1 cut(s) 458
BstAUI TGTACA 1 cut(s) 129
BstC8I GCNNGC 1 cut(s) 60
BstDEI CTNAG 2 cut(s) 163, 504
BstENI CCTNNNNNAGG 1 cut(s) 30
BstF5I GGATG 1 cut(s) 544
BstKTI GATC 3 cut(s) 145, 179, 412
BstMAI GTCTC 2 cut(s) 27, 210
BstMBI GATC 3 cut(s) 142, 176, 409
BstMWI GCNNNNNNNGC 4 cut(s) 68, 260, 458, 467
BstNI CCWGG 1 cut(s) 513
BstSCI CCNGG 1 cut(s) 511
BstSLI GKGCMC 1 cut(s) 472
BstV1I GCAGC 1 cut(s) 445
BstXI CCANNNNNNTGG 1 cut(s) 641
BsuI GTATCC 1 cut(s) 623
BtsCI GGATG 1 cut(s) 544
BtsIMutI CAGTG 1 cut(s) 108
Cac8I GCNNGC 1 cut(s) 60
CsiI ACCWGGT 1 cut(s) 511
Csp6I GTAC 2 cut(s) 130, 403
CviAII CATG 1 cut(s) 587
CviJI RGCY 9 cut(s) 85, 125, 295, 324, 391, 458, 475, 503, 617
CviKI_1 RGCY 9 cut(s) 85, 125, 295, 324, 391, 458, 475, 503, 617
CviQI GTAC 2 cut(s) 130, 403
DdeI CTNAG 2 cut(s) 163, 504
DpnI GATC 3 cut(s) 144, 178, 411
DpnII GATC 3 cut(s) 142, 176, 409
Eam1104I CTCTTC 3 cut(s) 71, 241, 329
EarI CTCTTC 3 cut(s) 71, 241, 329
EciI GGCGGA 1 cut(s) 561
Eco24I GRGCYC 1 cut(s) 297
EcoNI CCTNNNNNAGG 1 cut(s) 30
EcoRI GAATTC 1 cut(s) 168
EcoRII CCWGG 1 cut(s) 511
EcoT38I GRGCYC 1 cut(s) 297
Esp3I CGTCTC 1 cut(s) 27
FaeI CATG 1 cut(s) 590
FaiI YATR 7 cut(s) 81, 231, 408, 556, 572, 588, 621
FalI AAGNNNNNCTT 2 cut(s) 238, 270
FaqI GGGAC 1 cut(s) 151
FatI CATG 1 cut(s) 586
FbaI TGATCA 1 cut(s) 142
Fnu4HI GCNGC 2 cut(s) 6, 459
FokI GGATG 1 cut(s) 551
FriOI GRGCYC 1 cut(s) 297
Fsp4HI GCNGC 2 cut(s) 6, 459
FspBI CTAG 2 cut(s) 392, 626
GluI GCNGC 2 cut(s) 6, 459
Hin1II CATG 1 cut(s) 590
HinfI GANTC 2 cut(s) 302, 532
HphI GGTGA 3 cut(s) 43, 227, 343
Hpy166II GTNNAC 1 cut(s) 108
Hpy188I TCNGA 1 cut(s) 176
Hpy188III TCNNGA 4 cut(s) 243, 258, 273, 536
Hpy8I GTNNAC 1 cut(s) 108
HpyAV CCTTC 2 cut(s) 190, 433
HpyCH4III ACNGT 2 cut(s) 112, 215
HpyCH4IV ACGT 1 cut(s) 20
HpyCH4V TGCA 1 cut(s) 519
HpyF10VI GCNNNNNNNGC 4 cut(s) 68, 260, 458, 467
HpyF3I CTNAG 2 cut(s) 163, 504
HpySE526I ACGT 1 cut(s) 20
Hsp92II CATG 1 cut(s) 590
Ksp22I TGATCA 1 cut(s) 142
Kzo9I GATC 3 cut(s) 142, 176, 409
Lsp1109I GCAGC 1 cut(s) 445
LweI GCATC 1 cut(s) 241
MabI ACCWGGT 1 cut(s) 511
MaeI CTAG 2 cut(s) 392, 626
MaeII ACGT 1 cut(s) 20
MaeIII GTNAC 2 cut(s) 49, 190
MalI GATC 3 cut(s) 144, 178, 411
MboI GATC 3 cut(s) 142, 176, 409
MboII GAAGA 7 cut(s) 58, 128, 151, 258, 346, 493, 509
MhlI GDGCHC 2 cut(s) 297, 472
MluCI AATT 4 cut(s) 36, 119, 168, 520
MlyI GAGTC 2 cut(s) 296, 541
MnlI CCTC 6 cut(s) 211, 345, 458, 535, 544, 607
MseI TTAA 2 cut(s) 17, 308
MslI CAYNNNNRTG 1 cut(s) 213
MspR9I CCNGG 1 cut(s) 513
MvaI CCWGG 1 cut(s) 513
MwoI GCNNNNNNNGC 4 cut(s) 68, 260, 458, 467
NdeII GATC 3 cut(s) 142, 176, 409
NlaIII CATG 1 cut(s) 590
NmuCI GTSAC 2 cut(s) 49, 190
PkrI GCNGC 2 cut(s) 7, 460
PleI GAGTC 2 cut(s) 296, 540
PpsI GAGTC 2 cut(s) 296, 540
PsiI TTATAA 3 cut(s) 231, 572, 621
Psp6I CCWGG 1 cut(s) 511
PspGI CCWGG 1 cut(s) 511
RsaI GTAC 2 cut(s) 131, 404
RsaNI GTAC 2 cut(s) 130, 403
RseI CAYNNNNRTG 1 cut(s) 213
SaqAI TTAA 2 cut(s) 17, 308
SatI GCNGC 2 cut(s) 6, 459
Sau3AI GATC 3 cut(s) 142, 176, 409
SchI GAGTC 2 cut(s) 296, 541
ScrFI CCNGG 1 cut(s) 513
SduI GDGCHC 2 cut(s) 297, 472
SexAI ACCWGGT 1 cut(s) 511
SfaNI GCATC 1 cut(s) 241
SmiMI CAYNNNNRTG 1 cut(s) 213
Sse9I AATT 4 cut(s) 36, 119, 168, 520
SsiI CCGC 3 cut(s) 5, 312, 546
SspI AATATT 2 cut(s) 434, 576
SspMI CTAG 2 cut(s) 392, 626
StyD4I CCNGG 1 cut(s) 511
TaaI ACNGT 2 cut(s) 112, 215
TaiI ACGT 1 cut(s) 23
TaqI TCGA 1 cut(s) 186
TasI AATT 4 cut(s) 36, 119, 168, 520
TatI WGTACW 1 cut(s) 129
TauI GCSGC 1 cut(s) 8
Tru1I TTAA 2 cut(s) 17, 308
Tru9I TTAA 2 cut(s) 17, 308
TscAI CASTG 1 cut(s) 115
TseFI GTSAC 2 cut(s) 49, 190
TseI GCWGC 1 cut(s) 458
Tsp45I GTSAC 2 cut(s) 49, 190
TspRI CASTG 1 cut(s) 115
XagI CCTNNNNNAGG 1 cut(s) 30
XapI RAATTY 1 cut(s) 168
XspI CTAG 2 cut(s) 392, 626
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.