AT4G15610

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0010939
Physical Location & Seq
Forward (+)
8908763 .. 8910945
2183 bp
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UTR
Exon/CDS
Intron
AT4G15610.1

Sequence Viewer

Length: 582 bp
ATGGGTTATGAAACCAAATCGACTTTGGACACCGAGAGATCGACCGCGCCAGGAACCGGCACCACGACGAAGAGTTGTTCGATGACTCAAGTTGTCCTAAGATTTGTTCTGTTTGCAGCCACTTTGACATCAATAGTGGTAATGGTTACAAGTAAACAGACCAAGAATATATTCCTTCCTGGAACTCCCATTCGTATTCCTGCTGCAGAGTTCACCAACTCACCGGCTCTTATATACTTTGTGGTAGCATTATCCGTGGCATGCTTTTACAGCATTGTATCTACATTTGTCACCGTATCTGCTTTCAAGAAGCATTCCTGCTCTGCGGTTCTCTTGCTAAATCTTGCCATTATGGATGCGGTGATGGTGGGGATAGTGGCCTCAGCAACCGGGGCAGGAGGTGGAGTAGCTTACCTTGGTCTGAAGGGAAACAAAGAAGTTAGATGGGGCAAAATCTGTCATATCTACGACAAGTTTTGCCGTCATGTTGGAGGAGCAATCGCCGTCTCATTGTTTGCTTCGGTTGTCCTCCTCCTCCTATCCATCATCTCTGTCCTCTCCCTCTACAAGAAAATCCGTTGA

Protein Analysis

193

Amino Acids

20.52

Weight (kDa)

9.68

Isoelectric Point (pI)

29.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 24 - 176 3.3e-41 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0010939)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G06390 AT4G15610 AT4G15610
fragaria_vesca FvH4_4g17250
malus_domestica MD13G1173500.v1.1 MD16G1174700.v1.1
prunus_persica Prupe.1G141700_v2.0.a1 Prupe.1G141700_v2.0.a1
pyrus_communis pycom13g14910 pycom16g14660
rosa_chinensis RchiOBHm_Chr4g0421171
rosa_laevigata RLG00000007710
rosa_multiflora Rmu_sc0009290.1_g000005
rosa_roxburghii Rroxscaffold_5G00363470
rosa_rugosa Rorug04G0170000
rosa_samantha Rh4AG229700 Rh4BG232400 Rh4CG244000 Rh4DG228100
rosa_wichuraiana Rw4G020410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 59
AccII CGCG 1 cut(s) 47
AciI CCGC 3 cut(s) 45, 326, 359
AcuI CTGAAG 1 cut(s) 443
AfiI CCNNNNNNNGG 1 cut(s) 56
AgsI TTSAA 1 cut(s) 307
AjnI CCWGG 2 cut(s) 49, 178
AjuI GAANNNNNNNTTGG 2 cut(s) 155, 187
AluBI AGCT 1 cut(s) 410
AluI AGCT 1 cut(s) 410
Alw26I GTCTC 1 cut(s) 511
AoxI GGCC 1 cut(s) 378
ApeKI GCWGC 2 cut(s) 116, 203
Asp700I GAANNNNTTC 1 cut(s) 170
AspLEI GCGC 1 cut(s) 49
AsuC2I CCSGG 1 cut(s) 391
AsuHPI GGTGA 4 cut(s) 205, 213, 283, 373
BanI GGYRCC 1 cut(s) 59
BbvCI CCTCAGC 1 cut(s) 382
BbvI GCAGC 2 cut(s) 128, 190
BccI CCATC 3 cut(s) 358, 438, 551
BceAI ACGGC 2 cut(s) 465, 488
BciT130I CCWGG 2 cut(s) 51, 180
BcnI CCSGG 1 cut(s) 391
BcoDI GTCTC 1 cut(s) 511
BfmI CTRYAG 1 cut(s) 204
BisI GCNGC 2 cut(s) 117, 204
BlsI GCNGC 2 cut(s) 118, 205
Bme1390I CCNGG 3 cut(s) 51, 180, 391
BmiI GGNNCC 2 cut(s) 55, 61
BmrFI CCNGG 3 cut(s) 51, 180, 391
BmsI GCATC 1 cut(s) 346
Bpu10I CCTNAGC 1 cut(s) 382
BpuEI CTTGAG 1 cut(s) 72
BpuMI CCSGG 1 cut(s) 391
BsaJI CCNNGG 3 cut(s) 255, 390, 415
BsaXI ACNNNNNCTCC 2 cut(s) 390, 420
Bsc4I CCNNNNNNNGG 1 cut(s) 56
Bse118I RCCGGY 2 cut(s) 56, 223
BseBI CCWGG 2 cut(s) 51, 180
BseDI CCNNGG 3 cut(s) 255, 390, 415
BseGI GGATG 1 cut(s) 361
BseLI CCNNNNNNNGG 1 cut(s) 56
BseMII CTCAG 1 cut(s) 396
BseRI GAGGAG 3 cut(s) 507, 521, 524
BseXI GCAGC 2 cut(s) 128, 190
Bsh1236I CGCG 1 cut(s) 47
Bsh1285I CGRYCG 1 cut(s) 45
BshFI GGCC 1 cut(s) 380
BshNI GGYRCC 1 cut(s) 59
BsiEI CGRYCG 1 cut(s) 45
BsiSI CCGG 3 cut(s) 57, 224, 390
BslI CCNNNNNNNGG 1 cut(s) 56
BsmAI GTCTC 1 cut(s) 511
BsmBI CGTCTC 1 cut(s) 511
BsmI GAATGC 1 cut(s) 313
BsnI GGCC 1 cut(s) 380
Bsp143I GATC 1 cut(s) 38
BspACI CCGC 3 cut(s) 45, 326, 359
BspANI GGCC 1 cut(s) 380
BspCNI CTCAG 1 cut(s) 395
BspFNI CGCG 1 cut(s) 47
BspLI GGNNCC 2 cut(s) 55, 61
BspMAI CTGCAG 1 cut(s) 208
BspT107I GGYRCC 1 cut(s) 59
BsrFI RCCGGY 2 cut(s) 56, 223
BssAI RCCGGY 2 cut(s) 56, 223
BssECI CCNNGG 3 cut(s) 255, 390, 415
BssMI GATC 1 cut(s) 38
BssT1I CCWWGG 1 cut(s) 415
Bst2UI CCWGG 2 cut(s) 51, 180
Bst4CI ACNGT 1 cut(s) 295
Bst6I CTCTTC 1 cut(s) 65
BstC8I GCNNGC 1 cut(s) 262
BstDEI CTNAG 2 cut(s) 98, 382
BstDSI CCRYGG 1 cut(s) 255
BstF5I GGATG 1 cut(s) 361
BstFNI CGCG 1 cut(s) 47
BstHHI GCGC 1 cut(s) 49
BstKTI GATC 1 cut(s) 41
BstMAI GTCTC 1 cut(s) 511
BstMBI GATC 1 cut(s) 38
BstMCI CGRYCG 1 cut(s) 45
BstMWI GCNNNNNNNGC 2 cut(s) 270, 392
BstNI CCWGG 2 cut(s) 51, 180
BstNSI RCATGY 1 cut(s) 264
BstSCI CCNGG 3 cut(s) 49, 178, 389
BstSFI CTRYAG 1 cut(s) 204
BstUI CGCG 1 cut(s) 47
BstV1I GCAGC 2 cut(s) 128, 190
BsuRI GGCC 1 cut(s) 380
BtgI CCRYGG 1 cut(s) 255
BtsCI GGATG 1 cut(s) 361
Cac8I GCNNGC 1 cut(s) 262
CfoI GCGC 1 cut(s) 49
Cfr10I RCCGGY 2 cut(s) 56, 223
CviAII CATG 2 cut(s) 261, 485
CviJI RGCY 4 cut(s) 119, 227, 380, 410
CviKI_1 RGCY 4 cut(s) 119, 227, 380, 410
DdeI CTNAG 2 cut(s) 98, 382
DpnI GATC 1 cut(s) 40
DpnII GATC 1 cut(s) 38
Eam1104I CTCTTC 1 cut(s) 65
EarI CTCTTC 1 cut(s) 65
Eco130I CCWWGG 1 cut(s) 415
Eco57I CTGAAG 1 cut(s) 443
EcoRII CCWGG 2 cut(s) 49, 178
EcoT14I CCWWGG 1 cut(s) 415
ErhI CCWWGG 1 cut(s) 415
Esp3I CGTCTC 1 cut(s) 511
FaeI CATG 2 cut(s) 264, 488
FaiI YATR 8 cut(s) 9, 170, 233, 235, 262, 353, 462, 486
FatI CATG 2 cut(s) 260, 484
Fnu4HI GCNGC 2 cut(s) 117, 204
FokI GGATG 1 cut(s) 368
Fsp4HI GCNGC 2 cut(s) 117, 204
GlaI GCGC 1 cut(s) 48
GluI GCNGC 2 cut(s) 117, 204
HaeIII GGCC 1 cut(s) 380
HapII CCGG 3 cut(s) 57, 224, 390
HhaI GCGC 1 cut(s) 49
Hin1II CATG 2 cut(s) 264, 488
Hin6I GCGC 1 cut(s) 47
HinP1I GCGC 1 cut(s) 47
HinfI GANTC 1 cut(s) 85
HpaII CCGG 3 cut(s) 57, 224, 390
HphI GGTGA 4 cut(s) 205, 213, 283, 373
Hpy166II GTNNAC 2 cut(s) 155, 213
Hpy188I TCNGA 1 cut(s) 423
Hpy188III TCNNGA 1 cut(s) 307
Hpy8I GTNNAC 2 cut(s) 155, 213
Hpy99I CGWCG 1 cut(s) 70
HpyAV CCTTC 2 cut(s) 185, 418
HpyCH4III ACNGT 1 cut(s) 295
HpyCH4V TGCA 2 cut(s) 116, 206
HpyF10VI GCNNNNNNNGC 2 cut(s) 270, 392
HpyF3I CTNAG 2 cut(s) 98, 382
Hsp92II CATG 2 cut(s) 264, 488
HspAI GCGC 1 cut(s) 47
Kzo9I GATC 1 cut(s) 38
LmnI GCTCC 1 cut(s) 494
Lsp1109I GCAGC 2 cut(s) 128, 190
LweI GCATC 1 cut(s) 346
MaeIII GTNAC 2 cut(s) 145, 289
MalI GATC 1 cut(s) 40
MboI GATC 1 cut(s) 38
MboII GAAGA 1 cut(s) 82
MlyI GAGTC 1 cut(s) 79
MmeI TCCRAC 1 cut(s) 469
MnlI CCTC 8 cut(s) 391, 392, 485, 539, 542, 545, 566, 572
MroXI GAANNNNTTC 1 cut(s) 170
MspI CCGG 3 cut(s) 57, 224, 390
MspR9I CCNGG 3 cut(s) 51, 180, 391
Mva1269I GAATGC 1 cut(s) 313
MvaI CCWGG 2 cut(s) 51, 180
MvnI CGCG 1 cut(s) 47
MwoI GCNNNNNNNGC 2 cut(s) 270, 392
NciI CCSGG 1 cut(s) 391
NdeII GATC 1 cut(s) 38
NlaIII CATG 2 cut(s) 264, 488
NlaIV GGNNCC 2 cut(s) 55, 61
NmuCI GTSAC 1 cut(s) 289
NspI RCATGY 1 cut(s) 264
PaeI GCATGC 1 cut(s) 264
PctI GAATGC 1 cut(s) 313
PdmI GAANNNNTTC 1 cut(s) 170
PfoI TCCNGGA 1 cut(s) 178
PkrI GCNGC 2 cut(s) 118, 205
PleI GAGTC 1 cut(s) 79
PpsI GAGTC 1 cut(s) 79
Psp6I CCWGG 2 cut(s) 49, 178
PspGI CCWGG 2 cut(s) 49, 178
PspN4I GGNNCC 2 cut(s) 55, 61
PstI CTGCAG 1 cut(s) 208
SatI GCNGC 2 cut(s) 117, 204
Sau3AI GATC 1 cut(s) 38
SchI GAGTC 1 cut(s) 79
ScrFI CCNGG 3 cut(s) 51, 180, 391
SetI ASST 3 cut(s) 403, 412, 417
SfaNI GCATC 1 cut(s) 346
SfcI CTRYAG 1 cut(s) 204
SmlI CTYRAG 1 cut(s) 87
SmoI CTYRAG 1 cut(s) 87
SphI GCATGC 1 cut(s) 264
SsiI CCGC 3 cut(s) 45, 326, 359
StyD4I CCNGG 3 cut(s) 49, 178, 389
StyI CCWWGG 1 cut(s) 415
TaaI ACNGT 1 cut(s) 295
TaqI TCGA 3 cut(s) 20, 41, 80
TseFI GTSAC 1 cut(s) 289
TseI GCWGC 2 cut(s) 116, 203
Tsp45I GTSAC 1 cut(s) 289
TspDTI ATGAA 1 cut(s) 24
TspGWI ACGGA 2 cut(s) 244, 566
XceI RCATGY 1 cut(s) 264
XcmI CCANNNNNNNNNTGG 1 cut(s) 22
XmnI GAANNNNTTC 1 cut(s) 170
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.