Rw4G020410

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0010939
Physical Location & Seq
Forward (+)
44339129 .. 44341458
2330 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G020410.1

Sequence Viewer

Length: 606 bp
ATGGCATCGACGGATAAGCCAGCTAACCCGGAAACCGGTAAGGAAGTTCCACCACCAAAGGCCTCGTACGGAGACACGCCTCCTCCTCCTCCTCCTCCAAAAGCAGTTGACTTGTTTGCAGTCGATGTGGCACTTAGGTTATTGGTGTTTGCAGCATCACTAACAAGTGTATTGGTCATGGTAACTAGCAAGCAGACGGTATTCCGAGGCCCTATCCCGTTAAAAGCCAAGTTTGATCACTCTCCTGCCCTCATATACTTTGTAGTGGCACTATCAGTTGCAGGCCTCTATGGTATTCTTACTACACTAGCATCCCTTTCAGTCATCTGGAAGCCAATATTCTCAACCAAGTTCTTGCTCCACTTTGCATTTTGGGATGTGCTGATACTGGGTCTAGTGGCCTCTGCCACGGGTACCGCCGGGGGTGTTGCGTATATTGGATACAAGGGCAATGAACATTTGCAGTGGAACAAGGTTTGCTCTATTTTTGACAAGTACTGCAAACATCTAGCAGGCTCCCTCGCTACGTCGTTGTTTGCTTCGGTTTTGCTGGTCCTTCTGGTGTGGCTGTCAACTTTCTCACTCCACAAGAAAATTCCCAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0048037 GO:0051536 GO:0051540 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

201

Amino Acids

21.61

Weight (kDa)

9.52

Isoelectric Point (pI)

24.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 40 - 183 2.3e-35 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010939)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G06390 AT4G15610 AT4G15610
fragaria_vesca FvH4_4g17250
malus_domestica MD13G1173500.v1.1 MD16G1174700.v1.1
prunus_persica Prupe.1G141700_v2.0.a1 Prupe.1G141700_v2.0.a1
pyrus_communis pycom13g14910 pycom16g14660
rosa_chinensis RchiOBHm_Chr4g0421171
rosa_laevigata RLG00000007710
rosa_multiflora Rmu_sc0009290.1_g000005
rosa_roxburghii Rroxscaffold_5G00363470
rosa_rugosa Rorug04G0170000
rosa_samantha Rh4AG229700 Rh4BG232400 Rh4CG244000 Rh4DG228100
rosa_wichuraiana Rw4G020410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 413
AccB1I GGYRCC 1 cut(s) 413
AciI CCGC 1 cut(s) 417
AcsI RAATTY 1 cut(s) 594
AfaI GTAC 3 cut(s) 68, 415, 497
AfiI CCNNNNNNNGG 1 cut(s) 35
AgeI ACCGGT 1 cut(s) 35
AluBI AGCT 1 cut(s) 23
AluI AGCT 1 cut(s) 23
Alw26I GTCTC 1 cut(s) 66
AoxI GGCC 4 cut(s) 60, 208, 283, 399
ApeKI GCWGC 1 cut(s) 152
ApoI RAATTY 1 cut(s) 594
AsiGI ACCGGT 1 cut(s) 35
Asp718I GGTACC 1 cut(s) 413
AspS9I GGNCC 2 cut(s) 209, 553
AsuC2I CCSGG 2 cut(s) 29, 421
AvaII GGWCC 1 cut(s) 553
BanI GGYRCC 1 cut(s) 413
BbvI GCAGC 1 cut(s) 164
BciVI GTATCC 1 cut(s) 434
BclI TGATCA 1 cut(s) 235
BcnI CCSGG 2 cut(s) 29, 421
BcoDI GTCTC 1 cut(s) 66
BfaI CTAG 4 cut(s) 186, 308, 395, 509
BfuI GTATCC 1 cut(s) 434
BisI GCNGC 1 cut(s) 153
BlsI GCNGC 1 cut(s) 154
BmcAI AGTACT 1 cut(s) 497
Bme1390I CCNGG 2 cut(s) 29, 421
Bme18I GGWCC 1 cut(s) 553
BmgT120I GGNCC 2 cut(s) 209, 553
BmiI GGNNCC 2 cut(s) 415, 517
BmrFI CCNGG 2 cut(s) 29, 421
BmrI ACTGGG 1 cut(s) 398
BmsI GCATC 3 cut(s) 14, 164, 320
BmuI ACTGGG 1 cut(s) 398
BpuMI CCSGG 2 cut(s) 29, 421
BsaJI CCNNGG 3 cut(s) 205, 408, 420
BsaWI WCCGGW 1 cut(s) 35
BsaXI ACNNNNNCTCC 2 cut(s) 67, 97
Bsc4I CCNNNNNNNGG 1 cut(s) 35
Bse118I RCCGGY 1 cut(s) 35
Bse1I ACTGG 1 cut(s) 393
Bse3DI GCAATG 1 cut(s) 457
BseDI CCNNGG 3 cut(s) 205, 408, 420
BseGI GGATG 2 cut(s) 311, 382
BseLI CCNNNNNNNGG 1 cut(s) 35
BseMI GCAATG 1 cut(s) 457
BseNI ACTGG 1 cut(s) 393
BseRI GAGGAG 5 cut(s) 72, 75, 78, 81, 84
BseXI GCAGC 1 cut(s) 164
BshFI GGCC 4 cut(s) 62, 210, 285, 401
BshNI GGYRCC 1 cut(s) 413
BshTI ACCGGT 1 cut(s) 35
BsiSI CCGG 3 cut(s) 29, 36, 420
BsiWI CGTACG 1 cut(s) 66
BslI CCNNNNNNNGG 1 cut(s) 35
BsmAI GTCTC 1 cut(s) 66
BsnI GGCC 4 cut(s) 62, 210, 285, 401
Bsp143I GATC 1 cut(s) 235
BspACI CCGC 1 cut(s) 417
BspANI GGCC 4 cut(s) 62, 210, 285, 401
BspLI GGNNCC 2 cut(s) 415, 517
BspT107I GGYRCC 1 cut(s) 413
BsrDI GCAATG 1 cut(s) 457
BsrFI RCCGGY 1 cut(s) 35
BsrI ACTGG 1 cut(s) 393
BssAI RCCGGY 1 cut(s) 35
BssECI CCNNGG 3 cut(s) 205, 408, 420
BssMI GATC 1 cut(s) 235
Bst4CI ACNGT 1 cut(s) 199
BstC8I GCNNGC 4 cut(s) 21, 191, 283, 514
BstDEI CTNAG 1 cut(s) 134
BstDSI CCRYGG 1 cut(s) 408
BstF5I GGATG 2 cut(s) 311, 382
BstKTI GATC 1 cut(s) 238
BstMAI GTCTC 1 cut(s) 66
BstMBI GATC 1 cut(s) 235
BstSCI CCNGG 2 cut(s) 27, 419
BstV1I GCAGC 1 cut(s) 164
BsuI GTATCC 1 cut(s) 434
BsuRI GGCC 4 cut(s) 62, 210, 285, 401
BtgI CCRYGG 1 cut(s) 408
BtsCI GGATG 2 cut(s) 311, 382
BtsI GCAGTG 1 cut(s) 470
BtsIMutI CAGTG 1 cut(s) 470
Cac8I GCNNGC 4 cut(s) 21, 191, 283, 514
Cfr10I RCCGGY 1 cut(s) 35
Cfr13I GGNCC 2 cut(s) 209, 553
Csp6I GTAC 3 cut(s) 67, 414, 496
CspAI ACCGGT 1 cut(s) 35
CviAII CATG 1 cut(s) 178
CviQI GTAC 3 cut(s) 67, 414, 496
DdeI CTNAG 1 cut(s) 134
DpnI GATC 1 cut(s) 237
DpnII GATC 1 cut(s) 235
Eco147I AGGCCT 2 cut(s) 62, 285
Eco47I GGWCC 1 cut(s) 553
EcoO109I RGGNCCY 1 cut(s) 209
FaeI CATG 1 cut(s) 181
FaiI YATR 5 cut(s) 179, 254, 256, 291, 435
FatI CATG 1 cut(s) 177
FbaI TGATCA 1 cut(s) 235
Fnu4HI GCNGC 1 cut(s) 153
FokI GGATG 2 cut(s) 298, 389
Fsp4HI GCNGC 1 cut(s) 153
FspBI CTAG 4 cut(s) 186, 308, 395, 509
GluI GCNGC 1 cut(s) 153
HaeIII GGCC 4 cut(s) 62, 210, 285, 401
HapII CCGG 3 cut(s) 29, 36, 420
Hin1II CATG 1 cut(s) 181
HincII GTYRAC 2 cut(s) 109, 573
HindII GTYRAC 2 cut(s) 109, 573
HpaII CCGG 3 cut(s) 29, 36, 420
Hpy166II GTNNAC 2 cut(s) 109, 573
Hpy188I TCNGA 1 cut(s) 206
Hpy188III TCNNGA 1 cut(s) 328
Hpy8I GTNNAC 2 cut(s) 109, 573
Hpy99I CGWCG 2 cut(s) 13, 532
HpyAV CCTTC 1 cut(s) 566
HpyCH4III ACNGT 1 cut(s) 199
HpyCH4IV ACGT 1 cut(s) 527
HpyCH4V TGCA 6 cut(s) 119, 152, 281, 368, 463, 501
HpyF3I CTNAG 1 cut(s) 134
HpySE526I ACGT 1 cut(s) 527
Hsp92II CATG 1 cut(s) 181
KpnI GGTACC 1 cut(s) 417
Ksp22I TGATCA 1 cut(s) 235
Kzo9I GATC 1 cut(s) 235
LmnI GCTCC 2 cut(s) 363, 521
Lsp1109I GCAGC 1 cut(s) 164
LweI GCATC 3 cut(s) 14, 164, 320
MaeI CTAG 4 cut(s) 186, 308, 395, 509
MaeII ACGT 1 cut(s) 527
MaeIII GTNAC 1 cut(s) 181
MalI GATC 1 cut(s) 237
MboI GATC 1 cut(s) 235
MluCI AATT 1 cut(s) 594
MseI TTAA 1 cut(s) 221
MspI CCGG 3 cut(s) 29, 36, 420
MspR9I CCNGG 2 cut(s) 29, 421
NciI CCSGG 2 cut(s) 29, 421
NdeII GATC 1 cut(s) 235
NlaIII CATG 1 cut(s) 181
NlaIV GGNNCC 2 cut(s) 415, 517
PceI AGGCCT 2 cut(s) 62, 285
Pfl23II CGTACG 1 cut(s) 66
PinAI ACCGGT 1 cut(s) 35
PkrI GCNGC 1 cut(s) 154
PspLI CGTACG 1 cut(s) 66
PspN4I GGNNCC 2 cut(s) 415, 517
PspPI GGNCC 2 cut(s) 209, 553
RsaI GTAC 3 cut(s) 68, 415, 497
RsaNI GTAC 3 cut(s) 67, 414, 496
SaqAI TTAA 1 cut(s) 221
SatI GCNGC 1 cut(s) 153
Sau3AI GATC 1 cut(s) 235
Sau96I GGNCC 2 cut(s) 209, 553
ScaI AGTACT 1 cut(s) 497
ScrFI CCNGG 2 cut(s) 29, 421
SetI ASST 4 cut(s) 25, 140, 477, 530
SfaNI GCATC 3 cut(s) 14, 164, 320
SinI GGWCC 1 cut(s) 553
Sse9I AATT 1 cut(s) 594
SseBI AGGCCT 2 cut(s) 62, 285
SsiI CCGC 1 cut(s) 417
SspI AATATT 1 cut(s) 339
SspMI CTAG 4 cut(s) 186, 308, 395, 509
StuI AGGCCT 2 cut(s) 62, 285
StyD4I CCNGG 2 cut(s) 27, 419
TaaI ACNGT 1 cut(s) 199
TaiI ACGT 1 cut(s) 530
TaqI TCGA 2 cut(s) 8, 123
TasI AATT 1 cut(s) 594
TatI WGTACW 1 cut(s) 495
Tru1I TTAA 1 cut(s) 221
Tru9I TTAA 1 cut(s) 221
TscAI CASTG 1 cut(s) 470
TseI GCWGC 1 cut(s) 152
TspDTI ATGAA 1 cut(s) 468
TspGWI ACGGA 2 cut(s) 26, 84
TspRI CASTG 1 cut(s) 470
VpaK11BI GGWCC 1 cut(s) 553
XapI RAATTY 1 cut(s) 594
XspI CTAG 4 cut(s) 186, 308, 395, 509
ZrmI AGTACT 1 cut(s) 497
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.