MD13G1173500.v1.1

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0010939
Physical Location & Seq
Forward (+)
14254661 .. 14257251
2591 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1173500.v1.1.491

Sequence Viewer

Length: 606 bp
ATGGGAACCACGGATAAGCCAGCTGACCCAGAAACCGCCACCAAGGTAGTCCCACCGCCATCAAAGGAGGAGTCCGAAGCCACGCCTCCTCCCCCTAAACCCGACTACCTCTTTCTAGCTGATGTGGCTATCAGGTTCTTAGTGTTCGCAGCATCACTGACAAGTGTGTTGGTCATGGTCACTAGCAAGCAAACAGTATTCCGAGGTCGTTTCCCAGTTAAGGCCAAGTTTAATCACTCTCCTGCTTTCATATACTTTGTGGCAGCTTTATCGGTTGCAGGCCTCTATGCTCTTCTTACAACACTAGCATCCATTTCAGTCATCTGGAAGCCAATTTGCCCACCAAAGTTGTTGCTCCATTTTGCATTTCTTGATGTGCTGATACTTGGTTTGGTTGCCTCAGCCACCGGCACAGCCGGGGGTGTTGCTTATATCGGATACAAGGGTAATGAACATGTGATGTGGCAGAAGGTGTGCTCAGCTTTTGACAAGTACTGTAAGCACATAGCAGGCTCCCTCGCTACCTCGCTGTTTGCCTCGGTCTTGCTGGTTCTTCTGGTCTGGCTCTCAACTTTCACCCTTCACAAAAAAATTCGCCAAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0048037 GO:0051536 GO:0051540 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

202

Amino Acids

21.78

Weight (kDa)

9.36

Isoelectric Point (pI)

30.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 39 - 182 6.9e-37 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010939)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G06390 AT4G15610 AT4G15610
fragaria_vesca FvH4_4g17250
malus_domestica MD13G1173500.v1.1 MD16G1174700.v1.1
prunus_persica Prupe.1G141700_v2.0.a1 Prupe.1G141700_v2.0.a1
pyrus_communis pycom13g14910 pycom16g14660
rosa_chinensis RchiOBHm_Chr4g0421171
rosa_laevigata RLG00000007710
rosa_multiflora Rmu_sc0009290.1_g000005
rosa_roxburghii Rroxscaffold_5G00363470
rosa_rugosa Rorug04G0170000
rosa_samantha Rh4AG229700 Rh4BG232400 Rh4CG244000 Rh4DG228100
rosa_wichuraiana Rw4G020410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 36, 56
AcsI RAATTY 1 cut(s) 591
AfaI GTAC 1 cut(s) 494
AfiI CCNNNNNNNGG 1 cut(s) 220
AflIII ACRYGT 1 cut(s) 454
AluBI AGCT 4 cut(s) 23, 119, 266, 482
AluI AGCT 4 cut(s) 23, 119, 266, 482
Alw21I GWGCWC 1 cut(s) 479
AoxI GGCC 2 cut(s) 222, 280
ApeKI GCWGC 2 cut(s) 149, 263
ApoI RAATTY 1 cut(s) 591
ArsI GACNNNNNNTTYG 2 cut(s) 56, 88
AsuC2I CCSGG 1 cut(s) 418
AsuHPI GGTGA 1 cut(s) 568
BaeI ACNNNNGTAYC 2 cut(s) 430, 463
Bbv12I GWGCWC 1 cut(s) 479
BbvCI CCTCAGC 1 cut(s) 400
BbvI GCAGC 2 cut(s) 161, 275
BccI CCATC 1 cut(s) 67
BcgI CGANNNNNNTGC 2 cut(s) 252, 286
BciVI GTATCC 1 cut(s) 431
BcnI CCSGG 1 cut(s) 418
BfaI CTAG 3 cut(s) 116, 183, 305
BfuI GTATCC 1 cut(s) 431
BisI GCNGC 2 cut(s) 150, 264
BlpI GCTNAGC 1 cut(s) 478
BlsI GCNGC 2 cut(s) 151, 265
BmcAI AGTACT 1 cut(s) 494
Bme1390I CCNGG 1 cut(s) 418
BmiI GGNNCC 2 cut(s) 7, 514
BmrFI CCNGG 1 cut(s) 418
BmrI ACTGGG 1 cut(s) 209
BmsI GCATC 2 cut(s) 161, 317
BmuI ACTGGG 1 cut(s) 209
Bpu10I CCTNAGC 1 cut(s) 400
Bpu1102I GCTNAGC 1 cut(s) 478
BpuMI CCSGG 1 cut(s) 418
BsaJI CCNNGG 5 cut(s) 9, 42, 202, 417, 537
BsaXI ACNNNNNCTCC 2 cut(s) 73, 103
Bsc4I CCNNNNNNNGG 1 cut(s) 220
Bse118I RCCGGY 1 cut(s) 407
Bse1I ACTGG 1 cut(s) 215
BseDI CCNNGG 5 cut(s) 9, 42, 202, 417, 537
BseGI GGATG 1 cut(s) 308
BseLI CCNNNNNNNGG 1 cut(s) 220
BseMII CTCAG 2 cut(s) 414, 492
BseNI ACTGG 1 cut(s) 215
BseRI GAGGAG 2 cut(s) 78, 83
BseXI GCAGC 2 cut(s) 161, 275
BshFI GGCC 2 cut(s) 224, 282
BsiHKAI GWGCWC 1 cut(s) 479
BsiSI CCGG 2 cut(s) 408, 417
BslFI GGGAC 1 cut(s) 35
BslI CCNNNNNNNGG 1 cut(s) 220
BsmFI GGGAC 1 cut(s) 35
BsnI GGCC 2 cut(s) 224, 282
Bsp1286I GDGCHC 1 cut(s) 479
Bsp1720I GCTNAGC 1 cut(s) 478
BspACI CCGC 2 cut(s) 36, 56
BspANI GGCC 2 cut(s) 224, 282
BspCNI CTCAG 2 cut(s) 413, 491
BspLI GGNNCC 2 cut(s) 7, 514
BspQI GCTCTTC 1 cut(s) 297
BsrFI RCCGGY 1 cut(s) 407
BsrI ACTGG 1 cut(s) 215
BssAI RCCGGY 1 cut(s) 407
BssECI CCNNGG 5 cut(s) 9, 42, 202, 417, 537
BssT1I CCWWGG 1 cut(s) 42
Bst4CI ACNGT 2 cut(s) 196, 497
Bst6I CTCTTC 1 cut(s) 297
BstC8I GCNNGC 4 cut(s) 21, 188, 280, 511
BstDEI CTNAG 3 cut(s) 139, 400, 478
BstDSI CCRYGG 1 cut(s) 9
BstF5I GGATG 1 cut(s) 308
BstMWI GCNNNNNNNGC 1 cut(s) 125
BstNSI RCATGY 1 cut(s) 458
BstSCI CCNGG 1 cut(s) 416
BstV1I GCAGC 2 cut(s) 161, 275
BsuI GTATCC 1 cut(s) 431
BsuRI GGCC 2 cut(s) 224, 282
BtgI CCRYGG 1 cut(s) 9
BtsCI GGATG 1 cut(s) 308
BtsIMutI CAGTG 1 cut(s) 155
Cac8I GCNNGC 4 cut(s) 21, 188, 280, 511
Cfr10I RCCGGY 1 cut(s) 407
Csp6I GTAC 1 cut(s) 493
CspCI CAANNNNNGTGG 2 cut(s) 330, 365
CviAII CATG 2 cut(s) 175, 455
CviQI GTAC 1 cut(s) 493
DdeI CTNAG 3 cut(s) 139, 400, 478
Eam1104I CTCTTC 1 cut(s) 297
EarI CTCTTC 1 cut(s) 297
Eco130I CCWWGG 1 cut(s) 42
Eco147I AGGCCT 1 cut(s) 282
EcoT14I CCWWGG 1 cut(s) 42
ErhI CCWWGG 1 cut(s) 42
FaeI CATG 2 cut(s) 178, 458
FaiI YATR 7 cut(s) 176, 251, 253, 288, 432, 456, 506
FaqI GGGAC 1 cut(s) 35
FatI CATG 2 cut(s) 174, 454
Fnu4HI GCNGC 2 cut(s) 150, 264
FokI GGATG 1 cut(s) 295
Fsp4HI GCNGC 2 cut(s) 150, 264
FspBI CTAG 3 cut(s) 116, 183, 305
GluI GCNGC 2 cut(s) 150, 264
HaeIII GGCC 2 cut(s) 224, 282
HapII CCGG 2 cut(s) 408, 417
Hin1II CATG 2 cut(s) 178, 458
HinfI GANTC 1 cut(s) 71
HpaII CCGG 2 cut(s) 408, 417
HphI GGTGA 1 cut(s) 568
Hpy188I TCNGA 3 cut(s) 76, 203, 437
Hpy188III TCNNGA 2 cut(s) 325, 371
HpyAV CCTTC 2 cut(s) 463, 590
HpyCH4III ACNGT 2 cut(s) 196, 497
HpyCH4V TGCA 2 cut(s) 278, 365
HpyF10VI GCNNNNNNNGC 1 cut(s) 125
HpyF3I CTNAG 3 cut(s) 139, 400, 478
Hsp92II CATG 2 cut(s) 178, 458
LguI GCTCTTC 1 cut(s) 297
LmnI GCTCC 2 cut(s) 360, 518
Lsp1109I GCAGC 2 cut(s) 161, 275
LweI GCATC 2 cut(s) 161, 317
MaeI CTAG 3 cut(s) 116, 183, 305
MaeIII GTNAC 1 cut(s) 178
MboII GAAGA 2 cut(s) 284, 545
MhlI GDGCHC 1 cut(s) 479
MluCI AATT 2 cut(s) 333, 591
MlyI GAGTC 1 cut(s) 80
MseI TTAA 2 cut(s) 219, 231
MspA1I CMGCKG 1 cut(s) 23
MspI CCGG 2 cut(s) 408, 417
MspR9I CCNGG 1 cut(s) 418
MwoI GCNNNNNNNGC 1 cut(s) 125
NciI CCSGG 1 cut(s) 418
NlaIII CATG 2 cut(s) 178, 458
NlaIV GGNNCC 2 cut(s) 7, 514
NmuCI GTSAC 1 cut(s) 178
NspI RCATGY 1 cut(s) 458
PceI AGGCCT 1 cut(s) 282
PciI ACATGT 1 cut(s) 454
PciSI GCTCTTC 1 cut(s) 297
PkrI GCNGC 2 cut(s) 151, 265
PleI GAGTC 1 cut(s) 79
PpsI GAGTC 1 cut(s) 79
PscI ACATGT 1 cut(s) 454
PspN4I GGNNCC 2 cut(s) 7, 514
PvuII CAGCTG 1 cut(s) 23
RsaI GTAC 1 cut(s) 494
RsaNI GTAC 1 cut(s) 493
SapI GCTCTTC 1 cut(s) 297
SaqAI TTAA 2 cut(s) 219, 231
SatI GCNGC 2 cut(s) 150, 264
ScaI AGTACT 1 cut(s) 494
SchI GAGTC 1 cut(s) 80
ScrFI CCNGG 1 cut(s) 418
SduI GDGCHC 1 cut(s) 479
SfaNI GCATC 2 cut(s) 161, 317
Sse9I AATT 2 cut(s) 333, 591
SseBI AGGCCT 1 cut(s) 282
SsiI CCGC 2 cut(s) 36, 56
SspMI CTAG 3 cut(s) 116, 183, 305
StuI AGGCCT 1 cut(s) 282
StyD4I CCNGG 1 cut(s) 416
StyI CCWWGG 1 cut(s) 42
TaaI ACNGT 2 cut(s) 196, 497
TaqII GACCGA 1 cut(s) 529
TasI AATT 2 cut(s) 333, 591
TatI WGTACW 1 cut(s) 492
Tru1I TTAA 2 cut(s) 219, 231
Tru9I TTAA 2 cut(s) 219, 231
TscAI CASTG 1 cut(s) 162
TseFI GTSAC 1 cut(s) 178
TseI GCWGC 2 cut(s) 149, 263
Tsp45I GTSAC 1 cut(s) 178
TspDTI ATGAA 2 cut(s) 238, 465
TspGWI ACGGA 1 cut(s) 26
TspRI CASTG 1 cut(s) 162
XapI RAATTY 1 cut(s) 591
XceI RCATGY 1 cut(s) 458
XspI CTAG 3 cut(s) 116, 183, 305
ZrmI AGTACT 1 cut(s) 494
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.