Rroxscaffold_5G00363470

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0010939
Physical Location & Seq
Reverse (-)
44682657 .. 44685024
2368 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00363470.1

Sequence Viewer

Length: 711 bp
ATGGCATCGACGGATAAGCCAGCTAACCCGGAAACCGGTAAGGAAGTTCCACCACCAAAGGCCTCGTACGGAGACACGCCTCCTCCTCCAAAAGCAGTTGACTTGTTTGCAGTCGATGTGGCACTTAGGTTCTTGGTGTTTGCAGCATCACTAACAAGTGTATTGATCATGGTAACTAGCAAGCAGACGGAATTCCGAGGCCTTATCCCGTTAAAAGCCAAGTTTGATCACTCTCCTGCCTTCATATACTTTGTAGTGGCACTATCAGTTGCAGGCCTCTATAGTATTCTTACTACACTAGCATCCCTTTCAGTCATCTGGAAGCCAACATTCTCAACCAAGTTCTTGCTCCACTTTGCATTTTTGGGATGTGGTAAGCAAGCTCAGCTGCATTCTTTCTGTATATACTATATAGTATATTTGTACCAACAACAACACAAACAAACTGGACGGTACTGTGATACCCTTCTTTTGTTTATGATGCAGCTGATACTGGGTCTAGTGGCCTCTGCCACGGGTACCGCCGGGGGTGTTGCGTATATTGGATACAAGGGCAATGAACATTTGCAGTGGAACAAGGTTTGCTCTATTTTTGACAAGTACTGCAAACATCTAGCAGGCTCCCTCGCTACGTCGTTGTTTGCTTCGGTTTTGCTGGTCCTTCTGGTGTGGCTGTCAACTTTCTCACTCCACAAGAAAATTCCCAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0048037 GO:0051536 GO:0051540 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

236

Amino Acids

25.98

Weight (kDa)

9.37

Isoelectric Point (pI)

25.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 37 - 110 2.9e-15 Casparian strip membrane protein domain
CASP_dom PF04535 145 - 218 4.7e-16 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010939)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G06390 AT4G15610 AT4G15610
fragaria_vesca FvH4_4g17250
malus_domestica MD13G1173500.v1.1 MD16G1174700.v1.1
prunus_persica Prupe.1G141700_v2.0.a1 Prupe.1G141700_v2.0.a1
pyrus_communis pycom13g14910 pycom16g14660
rosa_chinensis RchiOBHm_Chr4g0421171
rosa_laevigata RLG00000007710
rosa_multiflora Rmu_sc0009290.1_g000005
rosa_roxburghii Rroxscaffold_5G00363470
rosa_rugosa Rorug04G0170000
rosa_samantha Rh4AG229700 Rh4BG232400 Rh4CG244000 Rh4DG228100
rosa_wichuraiana Rw4G020410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 518
AccB1I GGYRCC 1 cut(s) 518
AciI CCGC 1 cut(s) 522
AcsI RAATTY 2 cut(s) 191, 699
AfaI GTAC 5 cut(s) 68, 425, 455, 520, 602
AfiI CCNNNNNNNGG 1 cut(s) 35
AgeI ACCGGT 1 cut(s) 35
AluBI AGCT 4 cut(s) 23, 383, 388, 487
AluI AGCT 4 cut(s) 23, 383, 388, 487
Alw26I GTCTC 1 cut(s) 66
AoxI GGCC 4 cut(s) 60, 199, 274, 504
ApeKI GCWGC 3 cut(s) 143, 388, 484
ApoI RAATTY 2 cut(s) 191, 699
AsiGI ACCGGT 1 cut(s) 35
Asp718I GGTACC 1 cut(s) 518
AspS9I GGNCC 1 cut(s) 658
AsuC2I CCSGG 2 cut(s) 29, 526
AvaII GGWCC 1 cut(s) 658
BanI GGYRCC 1 cut(s) 518
BbvI GCAGC 3 cut(s) 155, 375, 496
BciVI GTATCC 1 cut(s) 539
BclI TGATCA 2 cut(s) 165, 226
BcnI CCSGG 2 cut(s) 29, 526
BcoDI GTCTC 1 cut(s) 66
BfaI CTAG 4 cut(s) 177, 299, 500, 614
BfmI CTRYAG 1 cut(s) 280
BfuI GTATCC 1 cut(s) 539
BisI GCNGC 3 cut(s) 144, 389, 485
BlpI GCTNAGC 1 cut(s) 384
BlsI GCNGC 3 cut(s) 145, 390, 486
BmcAI AGTACT 1 cut(s) 602
Bme1390I CCNGG 2 cut(s) 29, 526
Bme18I GGWCC 1 cut(s) 658
BmgT120I GGNCC 1 cut(s) 658
BmiI GGNNCC 2 cut(s) 520, 622
BmrFI CCNGG 2 cut(s) 29, 526
BmrI ACTGGG 1 cut(s) 503
BmsI GCATC 4 cut(s) 14, 155, 311, 471
BmuI ACTGGG 1 cut(s) 503
Bpu1102I GCTNAGC 1 cut(s) 384
BpuMI CCSGG 2 cut(s) 29, 526
BsaJI CCNNGG 3 cut(s) 196, 513, 525
BsaWI WCCGGW 1 cut(s) 35
BsaXI ACNNNNNCTCC 2 cut(s) 67, 97
Bsc4I CCNNNNNNNGG 1 cut(s) 35
Bse118I RCCGGY 1 cut(s) 35
Bse1I ACTGG 2 cut(s) 451, 498
Bse3DI GCAATG 1 cut(s) 562
BseDI CCNNGG 3 cut(s) 196, 513, 525
BseGI GGATG 2 cut(s) 302, 374
BseLI CCNNNNNNNGG 1 cut(s) 35
BseMI GCAATG 1 cut(s) 562
BseMII CTCAG 1 cut(s) 398
BseNI ACTGG 2 cut(s) 451, 498
BseRI GAGGAG 2 cut(s) 72, 75
BseXI GCAGC 3 cut(s) 155, 375, 496
BshFI GGCC 4 cut(s) 62, 201, 276, 506
BshNI GGYRCC 1 cut(s) 518
BshTI ACCGGT 1 cut(s) 35
BsiSI CCGG 3 cut(s) 29, 36, 525
BsiWI CGTACG 1 cut(s) 66
BslI CCNNNNNNNGG 1 cut(s) 35
BsmAI GTCTC 1 cut(s) 66
BsmI GAATGC 1 cut(s) 391
BsnI GGCC 4 cut(s) 62, 201, 276, 506
Bsp143I GATC 2 cut(s) 165, 226
Bsp1720I GCTNAGC 1 cut(s) 384
BspACI CCGC 1 cut(s) 522
BspANI GGCC 4 cut(s) 62, 201, 276, 506
BspCNI CTCAG 1 cut(s) 397
BspLI GGNNCC 2 cut(s) 520, 622
BspT107I GGYRCC 1 cut(s) 518
BsrDI GCAATG 1 cut(s) 562
BsrFI RCCGGY 1 cut(s) 35
BsrI ACTGG 2 cut(s) 451, 498
BssAI RCCGGY 1 cut(s) 35
BssECI CCNNGG 3 cut(s) 196, 513, 525
BssMI GATC 2 cut(s) 165, 226
Bst4CI ACNGT 2 cut(s) 453, 458
BstC8I GCNNGC 5 cut(s) 21, 182, 274, 381, 619
BstDEI CTNAG 2 cut(s) 125, 384
BstDSI CCRYGG 1 cut(s) 513
BstF5I GGATG 2 cut(s) 302, 374
BstKTI GATC 2 cut(s) 168, 229
BstMAI GTCTC 1 cut(s) 66
BstMBI GATC 2 cut(s) 165, 226
BstMWI GCNNNNNNNGC 1 cut(s) 385
BstSCI CCNGG 2 cut(s) 27, 524
BstSFI CTRYAG 1 cut(s) 280
BstV1I GCAGC 3 cut(s) 155, 375, 496
BsuI GTATCC 1 cut(s) 539
BsuRI GGCC 4 cut(s) 62, 201, 276, 506
BtgI CCRYGG 1 cut(s) 513
BtsCI GGATG 2 cut(s) 302, 374
BtsI GCAGTG 1 cut(s) 575
BtsIMutI CAGTG 1 cut(s) 575
Cac8I GCNNGC 5 cut(s) 21, 182, 274, 381, 619
Cfr10I RCCGGY 1 cut(s) 35
Cfr13I GGNCC 1 cut(s) 658
Csp6I GTAC 5 cut(s) 67, 424, 454, 519, 601
CspAI ACCGGT 1 cut(s) 35
CviAII CATG 1 cut(s) 169
CviQI GTAC 5 cut(s) 67, 424, 454, 519, 601
DdeI CTNAG 2 cut(s) 125, 384
DpnI GATC 2 cut(s) 167, 228
DpnII GATC 2 cut(s) 165, 226
Eco147I AGGCCT 3 cut(s) 62, 201, 276
Eco47I GGWCC 1 cut(s) 658
EcoRI GAATTC 1 cut(s) 191
FaeI CATG 1 cut(s) 172
FatI CATG 1 cut(s) 168
FbaI TGATCA 2 cut(s) 165, 226
Fnu4HI GCNGC 3 cut(s) 144, 389, 485
FokI GGATG 2 cut(s) 289, 381
Fsp4HI GCNGC 3 cut(s) 144, 389, 485
FspBI CTAG 4 cut(s) 177, 299, 500, 614
GluI GCNGC 3 cut(s) 144, 389, 485
HaeIII GGCC 4 cut(s) 62, 201, 276, 506
HapII CCGG 3 cut(s) 29, 36, 525
Hin1II CATG 1 cut(s) 172
HincII GTYRAC 2 cut(s) 100, 678
HindII GTYRAC 2 cut(s) 100, 678
HpaII CCGG 3 cut(s) 29, 36, 525
Hpy166II GTNNAC 2 cut(s) 100, 678
Hpy188I TCNGA 1 cut(s) 197
Hpy188III TCNNGA 1 cut(s) 319
Hpy8I GTNNAC 2 cut(s) 100, 678
Hpy99I CGWCG 2 cut(s) 13, 637
HpyAV CCTTC 3 cut(s) 250, 476, 671
HpyCH4III ACNGT 2 cut(s) 453, 458
HpyCH4IV ACGT 1 cut(s) 632
HpyCH4V TGCA 8 cut(s) 110, 143, 272, 359, 391, 484, 568, 606
HpyF10VI GCNNNNNNNGC 1 cut(s) 385
HpyF3I CTNAG 2 cut(s) 125, 384
HpySE526I ACGT 1 cut(s) 632
Hsp92II CATG 1 cut(s) 172
KpnI GGTACC 1 cut(s) 522
Ksp22I TGATCA 2 cut(s) 165, 226
Kzo9I GATC 2 cut(s) 165, 226
LmnI GCTCC 2 cut(s) 354, 626
Lsp1109I GCAGC 3 cut(s) 155, 375, 496
LweI GCATC 4 cut(s) 14, 155, 311, 471
MaeI CTAG 4 cut(s) 177, 299, 500, 614
MaeII ACGT 1 cut(s) 632
MaeIII GTNAC 1 cut(s) 172
MalI GATC 2 cut(s) 167, 228
MboI GATC 2 cut(s) 165, 226
MluCI AATT 2 cut(s) 191, 699
MnlI CCTC 8 cut(s) 73, 90, 93, 96, 191, 287, 517, 635
MseI TTAA 1 cut(s) 212
MspA1I CMGCKG 2 cut(s) 388, 487
MspI CCGG 3 cut(s) 29, 36, 525
MspR9I CCNGG 2 cut(s) 29, 526
Mva1269I GAATGC 1 cut(s) 391
MwoI GCNNNNNNNGC 1 cut(s) 385
NciI CCSGG 2 cut(s) 29, 526
NdeII GATC 2 cut(s) 165, 226
NlaIII CATG 1 cut(s) 172
NlaIV GGNNCC 2 cut(s) 520, 622
PceI AGGCCT 3 cut(s) 62, 201, 276
PctI GAATGC 1 cut(s) 391
Pfl23II CGTACG 1 cut(s) 66
PinAI ACCGGT 1 cut(s) 35
PkrI GCNGC 3 cut(s) 145, 390, 486
PspLI CGTACG 1 cut(s) 66
PspN4I GGNNCC 2 cut(s) 520, 622
PspPI GGNCC 1 cut(s) 658
PvuII CAGCTG 2 cut(s) 388, 487
RsaI GTAC 5 cut(s) 68, 425, 455, 520, 602
RsaNI GTAC 5 cut(s) 67, 424, 454, 519, 601
SaqAI TTAA 1 cut(s) 212
SatI GCNGC 3 cut(s) 144, 389, 485
Sau3AI GATC 2 cut(s) 165, 226
Sau96I GGNCC 1 cut(s) 658
ScaI AGTACT 1 cut(s) 602
ScrFI CCNGG 2 cut(s) 29, 526
SetI ASST 7 cut(s) 25, 131, 385, 390, 489, 582, 635
SfaNI GCATC 4 cut(s) 14, 155, 311, 471
SfcI CTRYAG 1 cut(s) 280
SinI GGWCC 1 cut(s) 658
Sse9I AATT 2 cut(s) 191, 699
SseBI AGGCCT 3 cut(s) 62, 201, 276
SsiI CCGC 1 cut(s) 522
SspMI CTAG 4 cut(s) 177, 299, 500, 614
StuI AGGCCT 3 cut(s) 62, 201, 276
StyD4I CCNGG 2 cut(s) 27, 524
TaaI ACNGT 2 cut(s) 453, 458
TaiI ACGT 1 cut(s) 635
TaqI TCGA 2 cut(s) 8, 114
TasI AATT 2 cut(s) 191, 699
TatI WGTACW 1 cut(s) 600
Tru1I TTAA 1 cut(s) 212
Tru9I TTAA 1 cut(s) 212
TscAI CASTG 1 cut(s) 575
TseI GCWGC 3 cut(s) 143, 388, 484
TspDTI ATGAA 2 cut(s) 232, 573
TspGWI ACGGA 3 cut(s) 26, 84, 203
TspRI CASTG 1 cut(s) 575
VpaK11BI GGWCC 1 cut(s) 658
XapI RAATTY 2 cut(s) 191, 699
XspI CTAG 4 cut(s) 177, 299, 500, 614
ZrmI AGTACT 1 cut(s) 602
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.