MD16G1174700.v1.1

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0010939
Physical Location & Seq
Forward (+)
14853544 .. 14858358
4815 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1174700.v1.1.491

Sequence Viewer

Length: 591 bp
ATGGAAACCAAGGATAAGCCAGCTGACCCGGAAGTCCCGCCGCCATCAAAGGAGGAGCCCGAAGCCAAGCCTCCTCCTCCCCCCAAACCCGACTACCTCTTTCTAGTTGATGTGGCTCTCAGGTTCATAGTGTTTGCAGCATCACTGACAAGTGTGTTGGTCATGGTCACCAGCAAGCAAACAGTATACCGAGGTCCTTTCCCAGTTCGGGCCAAGTTTAATCACTCTCCCGCCTTCACATACTTTGTGGTGGCTCTATCAGTTGCAGGCCTCTATGCTCTTCTTACAACACTAGCATCCATTTCAGTCATCTGGAAGCCAATTTTCCCACCAAAGTTGTTGCTCCATTTTGTATTTCTTGATATACTGATACTGGGTTTGGTCGCCTCAGCCACCGGCACAGCCGGGGGTGTTGCATATATCGGATACAAGGGTAATGAACATGTGATGTGGCAGAAGATATGCTCTGTTTTCGACAAGTTCTGTAAGCATATAGTAGGCGCCCTCGCTACCTCGCTGTTTGCCTCAGTTTTGCTGGTTCTTCTGGTCTGGCTCTCCACTTTCACCCTCCACAAAAAAATTCGCCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0048037 GO:0051536 GO:0051540 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

197

Amino Acids

21.55

Weight (kDa)

9.44

Isoelectric Point (pI)

31.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 35 - 178 3.7e-37 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010939)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G06390 AT4G15610 AT4G15610
fragaria_vesca FvH4_4g17250
malus_domestica MD13G1173500.v1.1 MD16G1174700.v1.1
prunus_persica Prupe.1G141700_v2.0.a1 Prupe.1G141700_v2.0.a1
pyrus_communis pycom13g14910 pycom16g14660
rosa_chinensis RchiOBHm_Chr4g0421171
rosa_laevigata RLG00000007710
rosa_multiflora Rmu_sc0009290.1_g000005
rosa_roxburghii Rroxscaffold_5G00363470
rosa_rugosa Rorug04G0170000
rosa_samantha Rh4AG229700 Rh4BG232400 Rh4CG244000 Rh4DG228100
rosa_wichuraiana Rw4G020410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 32
AccB1I GGYRCC 1 cut(s) 500
AccI GTMKAC 1 cut(s) 186
AciI CCGC 3 cut(s) 38, 41, 231
AcsI RAATTY 1 cut(s) 579
AcyI GRCGYC 1 cut(s) 501
AfiI CCNNNNNNNGG 1 cut(s) 208
AflIII ACRYGT 1 cut(s) 442
AluBI AGCT 1 cut(s) 23
AluI AGCT 1 cut(s) 23
AoxI GGCC 2 cut(s) 210, 268
ApeKI GCWGC 1 cut(s) 137
ApoI RAATTY 1 cut(s) 579
AspLEI GCGC 1 cut(s) 503
AspS9I GGNCC 2 cut(s) 194, 210
AsuC2I CCSGG 2 cut(s) 29, 406
AsuHPI GGTGA 2 cut(s) 160, 556
AvaII GGWCC 1 cut(s) 194
BaeI ACNNNNGTAYC 2 cut(s) 418, 451
BanI GGYRCC 1 cut(s) 500
BanII GRGCYC 1 cut(s) 60
BbvCI CCTCAGC 1 cut(s) 388
BbvI GCAGC 1 cut(s) 149
BccI CCATC 1 cut(s) 52
BciVI GTATCC 1 cut(s) 419
BcnI CCSGG 2 cut(s) 29, 406
BfaI CTAG 2 cut(s) 104, 293
BfoI RGCGCY 1 cut(s) 504
BfuI GTATCC 1 cut(s) 419
BisI GCNGC 2 cut(s) 41, 138
BlsI GCNGC 2 cut(s) 42, 139
Bme1390I CCNGG 2 cut(s) 29, 406
Bme18I GGWCC 1 cut(s) 194
BmgT120I GGNCC 2 cut(s) 194, 210
BmiI GGNNCC 2 cut(s) 57, 502
BmrFI CCNGG 2 cut(s) 29, 406
BmrI ACTGGG 2 cut(s) 197, 383
BmsI GCATC 2 cut(s) 149, 305
BmuI ACTGGG 2 cut(s) 197, 383
Bpu10I CCTNAGC 1 cut(s) 388
BpuMI CCSGG 2 cut(s) 29, 406
BsaHI GRCGYC 1 cut(s) 501
BsaJI CCNNGG 3 cut(s) 9, 190, 405
Bsc4I CCNNNNNNNGG 1 cut(s) 208
Bse118I RCCGGY 1 cut(s) 395
Bse1I ACTGG 3 cut(s) 203, 378, 586
BseDI CCNNGG 3 cut(s) 9, 190, 405
BseGI GGATG 1 cut(s) 296
BseLI CCNNNNNNNGG 1 cut(s) 208
BseMII CTCAG 3 cut(s) 133, 402, 540
BseNI ACTGG 3 cut(s) 203, 378, 586
BseRI GAGGAG 3 cut(s) 63, 66, 68
BseXI GCAGC 1 cut(s) 149
BshFI GGCC 2 cut(s) 212, 270
BshNI GGYRCC 1 cut(s) 500
BsiSI CCGG 3 cut(s) 29, 396, 405
BslFI GGGAC 1 cut(s) 20
BslI CCNNNNNNNGG 1 cut(s) 208
BsmFI GGGAC 1 cut(s) 20
BsnI GGCC 2 cut(s) 212, 270
Bsp1286I GDGCHC 1 cut(s) 60
BspACI CCGC 3 cut(s) 38, 41, 231
BspANI GGCC 2 cut(s) 212, 270
BspCNI CTCAG 3 cut(s) 132, 401, 539
BspLI GGNNCC 2 cut(s) 57, 502
BspQI GCTCTTC 1 cut(s) 285
BspT107I GGYRCC 1 cut(s) 500
BsrFI RCCGGY 1 cut(s) 395
BsrI ACTGG 3 cut(s) 203, 378, 586
BssAI RCCGGY 1 cut(s) 395
BssECI CCNNGG 3 cut(s) 9, 190, 405
BssNAI GTATAC 1 cut(s) 187
BssNI GRCGYC 1 cut(s) 501
BssT1I CCWWGG 1 cut(s) 9
Bst1107I GTATAC 1 cut(s) 187
Bst4CI ACNGT 1 cut(s) 184
Bst6I CTCTTC 1 cut(s) 285
BstACI GRCGYC 1 cut(s) 501
BstC8I GCNNGC 3 cut(s) 21, 176, 268
BstDEI CTNAG 3 cut(s) 119, 388, 526
BstEII GGTNACC 1 cut(s) 166
BstF5I GGATG 1 cut(s) 296
BstH2I RGCGCY 1 cut(s) 504
BstHHI GCGC 1 cut(s) 503
BstNSI RCATGY 1 cut(s) 446
BstPI GGTNACC 1 cut(s) 166
BstSCI CCNGG 2 cut(s) 27, 404
BstV1I GCAGC 1 cut(s) 149
BstZ17I GTATAC 1 cut(s) 187
BsuI GTATCC 1 cut(s) 419
BsuRI GGCC 2 cut(s) 212, 270
BtsCI GGATG 1 cut(s) 296
BtsIMutI CAGTG 1 cut(s) 143
Cac8I GCNNGC 3 cut(s) 21, 176, 268
CfoI GCGC 1 cut(s) 503
Cfr10I RCCGGY 1 cut(s) 395
Cfr13I GGNCC 2 cut(s) 194, 210
CspCI CAANNNNNGTGG 2 cut(s) 318, 353
CviAII CATG 2 cut(s) 163, 443
DdeI CTNAG 3 cut(s) 119, 388, 526
DinI GGCGCC 1 cut(s) 502
DrdI GACNNNNNNGTC 1 cut(s) 32
DseDI GACNNNNNNGTC 1 cut(s) 32
Eam1104I CTCTTC 1 cut(s) 285
EarI CTCTTC 1 cut(s) 285
Eco130I CCWWGG 1 cut(s) 9
Eco147I AGGCCT 1 cut(s) 270
Eco24I GRGCYC 1 cut(s) 60
Eco47I GGWCC 1 cut(s) 194
Eco91I GGTNACC 1 cut(s) 166
EcoO109I RGGNCCY 1 cut(s) 194
EcoO65I GGTNACC 1 cut(s) 166
EcoT14I CCWWGG 1 cut(s) 9
EcoT38I GRGCYC 1 cut(s) 60
EgeI GGCGCC 1 cut(s) 502
EheI GGCGCC 1 cut(s) 502
ErhI CCWWGG 1 cut(s) 9
FaeI CATG 2 cut(s) 166, 446
FaqI GGGAC 1 cut(s) 20
FatI CATG 2 cut(s) 162, 442
FauI CCCGC 2 cut(s) 45, 238
FblI GTMKAC 1 cut(s) 186
Fnu4HI GCNGC 2 cut(s) 41, 138
FokI GGATG 1 cut(s) 283
FriOI GRGCYC 1 cut(s) 60
Fsp4HI GCNGC 2 cut(s) 41, 138
FspBI CTAG 2 cut(s) 104, 293
GlaI GCGC 1 cut(s) 502
GluI GCNGC 2 cut(s) 41, 138
HaeII RGCGCY 1 cut(s) 504
HaeIII GGCC 2 cut(s) 212, 270
HapII CCGG 3 cut(s) 29, 396, 405
HhaI GCGC 1 cut(s) 503
Hin1I GRCGYC 1 cut(s) 501
Hin1II CATG 2 cut(s) 166, 446
Hin6I GCGC 1 cut(s) 501
HinP1I GCGC 1 cut(s) 501
HpaII CCGG 3 cut(s) 29, 396, 405
HphI GGTGA 2 cut(s) 160, 556
Hpy166II GTNNAC 1 cut(s) 187
Hpy188I TCNGA 1 cut(s) 425
Hpy188III TCNNGA 2 cut(s) 313, 359
Hpy8I GTNNAC 1 cut(s) 187
HpyAV CCTTC 1 cut(s) 244
HpyCH4III ACNGT 1 cut(s) 184
HpyCH4V TGCA 3 cut(s) 137, 266, 416
HpyF3I CTNAG 3 cut(s) 119, 388, 526
Hsp92I GRCGYC 1 cut(s) 501
Hsp92II CATG 2 cut(s) 166, 446
HspAI GCGC 1 cut(s) 501
KasI GGCGCC 1 cut(s) 500
LguI GCTCTTC 1 cut(s) 285
LmnI GCTCC 2 cut(s) 55, 348
Lsp1109I GCAGC 1 cut(s) 149
LweI GCATC 2 cut(s) 149, 305
MaeI CTAG 2 cut(s) 104, 293
MaeIII GTNAC 1 cut(s) 166
MboII GAAGA 3 cut(s) 272, 469, 533
MhlI GDGCHC 1 cut(s) 60
MluCI AATT 2 cut(s) 321, 579
Mly113I GGCGCC 1 cut(s) 501
MseI TTAA 1 cut(s) 219
MspA1I CMGCKG 1 cut(s) 23
MspI CCGG 3 cut(s) 29, 396, 405
MspR9I CCNGG 2 cut(s) 29, 406
NarI GGCGCC 1 cut(s) 501
NciI CCSGG 2 cut(s) 29, 406
NlaIII CATG 2 cut(s) 166, 446
NlaIV GGNNCC 2 cut(s) 57, 502
NmuCI GTSAC 1 cut(s) 166
NspI RCATGY 1 cut(s) 446
PceI AGGCCT 1 cut(s) 270
PciI ACATGT 1 cut(s) 442
PciSI GCTCTTC 1 cut(s) 285
PkrI GCNGC 2 cut(s) 42, 139
PluTI GGCGCC 1 cut(s) 504
PpuMI RGGWCCY 1 cut(s) 194
PscI ACATGT 1 cut(s) 442
Psp5II RGGWCCY 1 cut(s) 194
PspEI GGTNACC 1 cut(s) 166
PspN4I GGNNCC 2 cut(s) 57, 502
PspPI GGNCC 2 cut(s) 194, 210
PspPPI RGGWCCY 1 cut(s) 194
PvuII CAGCTG 1 cut(s) 23
SapI GCTCTTC 1 cut(s) 285
SaqAI TTAA 1 cut(s) 219
SatI GCNGC 2 cut(s) 41, 138
Sau96I GGNCC 2 cut(s) 194, 210
ScrFI CCNGG 2 cut(s) 29, 406
SduI GDGCHC 1 cut(s) 60
SetI ASST 5 cut(s) 25, 99, 125, 196, 515
SfaNI GCATC 2 cut(s) 149, 305
SfoI GGCGCC 1 cut(s) 502
SinI GGWCC 1 cut(s) 194
Sse9I AATT 2 cut(s) 321, 579
SseBI AGGCCT 1 cut(s) 270
SsiI CCGC 3 cut(s) 38, 41, 231
SspDI GGCGCC 1 cut(s) 500
SspMI CTAG 2 cut(s) 104, 293
StuI AGGCCT 1 cut(s) 270
StyD4I CCNGG 2 cut(s) 27, 404
StyI CCWWGG 1 cut(s) 9
TaaI ACNGT 1 cut(s) 184
TaqI TCGA 1 cut(s) 474
TasI AATT 2 cut(s) 321, 579
TauI GCSGC 1 cut(s) 43
Tru1I TTAA 1 cut(s) 219
Tru9I TTAA 1 cut(s) 219
TscAI CASTG 1 cut(s) 150
TseFI GTSAC 1 cut(s) 166
TseI GCWGC 1 cut(s) 137
Tsp45I GTSAC 1 cut(s) 166
TspDTI ATGAA 2 cut(s) 115, 453
TspRI CASTG 1 cut(s) 150
VpaK11BI GGWCC 1 cut(s) 194
XapI RAATTY 1 cut(s) 579
XceI RCATGY 1 cut(s) 446
XmiI GTMKAC 1 cut(s) 186
XspI CTAG 2 cut(s) 104, 293
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.