FvH4_4g17250

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0010939
Physical Location & Seq
Reverse (-)
21227259 .. 21230126
2868 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g17250.t1

Sequence Viewer

Length: 597 bp
ATGGCATCGACGGATAAGCCAGCTGACCCGGAAACCGGTAAGGAAGTTCTGCCATCAAAGGCCTCATGCGACGACACGCCTCCTCCTCCAAAAGCAGTTGACTTGTTTGCAGTTGATGTGGCTCTTAGGTTCTTGGTGTTTGCAGCATCACTAACAAGTGTATTGGTCATTGTAACTAGCAAGCAGACGGTATTCCAAGGTCCTATCCCATTTGAAGCCAAGTTTGAGAACTCTCCTGCCTTCATATACTTTATAGTGGCACTATCAGTTGCAGGCCTCTATAGTATTCTTACTACACTAGCATCCCTTTCAGTCATCTGGAAGCCAATATTCTCAACCAAGTTATTGCTCCACTTTGCATTTTGGGATGTGCTGATACTGGGTCTAGTGGCCTCGGCCACTGGCACTGCCGGCGGTGTTGCATATATCGGATACAAGGGCAATGAACATGTGCAGTGGAACAAGGTTTGCTCTATTTTTGACGAGTACTGCAAACATATAGCAGGCTCCCTCGCTTCATCGTTGTTTGCTTCGGTTTTGCTAGTCCTTCTGGTGTGGTTGTCAACTTTCTCACTCCATAAGAAAATTCCCAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0048037 GO:0051536 GO:0051540 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

199

Amino Acids

21.35

Weight (kDa)

7.71

Isoelectric Point (pI)

26.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 37 - 180 3.6e-36 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010939)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G06390 AT4G15610 AT4G15610
fragaria_vesca FvH4_4g17250
malus_domestica MD13G1173500.v1.1 MD16G1174700.v1.1
prunus_persica Prupe.1G141700_v2.0.a1 Prupe.1G141700_v2.0.a1
pyrus_communis pycom13g14910 pycom16g14660
rosa_chinensis RchiOBHm_Chr4g0421171
rosa_laevigata RLG00000007710
rosa_multiflora Rmu_sc0009290.1_g000005
rosa_roxburghii Rroxscaffold_5G00363470
rosa_rugosa Rorug04G0170000
rosa_samantha Rh4AG229700 Rh4BG232400 Rh4CG244000 Rh4DG228100
rosa_wichuraiana Rw4G020410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 414
AcoI YGGCCR 1 cut(s) 396
AcsI RAATTY 1 cut(s) 585
AfaI GTAC 1 cut(s) 488
AfiI CCNNNNNNNGG 1 cut(s) 35
AflIII ACRYGT 1 cut(s) 448
AgeI ACCGGT 1 cut(s) 35
AgsI TTSAA 1 cut(s) 215
AluBI AGCT 1 cut(s) 23
AluI AGCT 1 cut(s) 23
AoxI GGCC 4 cut(s) 60, 274, 390, 396
ApeKI GCWGC 1 cut(s) 143
ApoI RAATTY 1 cut(s) 585
AsiGI ACCGGT 1 cut(s) 35
AspS9I GGNCC 1 cut(s) 200
AsuC2I CCSGG 1 cut(s) 29
AvaII GGWCC 1 cut(s) 200
BbvI GCAGC 1 cut(s) 155
BccI CCATC 1 cut(s) 61
BciVI GTATCC 1 cut(s) 425
BcnI CCSGG 1 cut(s) 29
BfaI CTAG 4 cut(s) 177, 299, 386, 542
BfmI CTRYAG 1 cut(s) 280
BfuI GTATCC 1 cut(s) 425
BisI GCNGC 1 cut(s) 144
BlsI GCNGC 1 cut(s) 145
BmcAI AGTACT 1 cut(s) 488
Bme1390I CCNGG 1 cut(s) 29
Bme18I GGWCC 1 cut(s) 200
BmgT120I GGNCC 1 cut(s) 200
BmiI GGNNCC 1 cut(s) 508
BmrFI CCNGG 1 cut(s) 29
BmrI ACTGGG 1 cut(s) 389
BmsI GCATC 3 cut(s) 14, 155, 311
BmuI ACTGGG 1 cut(s) 389
BpuMI CCSGG 1 cut(s) 29
BsaJI CCNNGG 2 cut(s) 196, 393
BsaWI WCCGGW 1 cut(s) 35
BsaXI ACNNNNNCTCC 2 cut(s) 67, 97
Bsc4I CCNNNNNNNGG 1 cut(s) 35
Bse118I RCCGGY 2 cut(s) 35, 410
Bse1I ACTGG 2 cut(s) 384, 406
Bse3DI GCAATG 1 cut(s) 448
BseDI CCNNGG 2 cut(s) 196, 393
BseGI GGATG 2 cut(s) 302, 373
BseLI CCNNNNNNNGG 1 cut(s) 35
BseMI GCAATG 1 cut(s) 448
BseNI ACTGG 2 cut(s) 384, 406
BseRI GAGGAG 2 cut(s) 72, 75
BseXI GCAGC 1 cut(s) 155
BsgI GTGCAG 1 cut(s) 473
BshFI GGCC 4 cut(s) 62, 276, 392, 398
BshTI ACCGGT 1 cut(s) 35
BsiSI CCGG 3 cut(s) 29, 36, 411
BslI CCNNNNNNNGG 1 cut(s) 35
BsnI GGCC 4 cut(s) 62, 276, 392, 398
BspACI CCGC 1 cut(s) 414
BspANI GGCC 4 cut(s) 62, 276, 392, 398
BspLI GGNNCC 1 cut(s) 508
BsrDI GCAATG 1 cut(s) 448
BsrFI RCCGGY 2 cut(s) 35, 410
BsrI ACTGG 2 cut(s) 384, 406
BssAI RCCGGY 2 cut(s) 35, 410
BssECI CCNNGG 2 cut(s) 196, 393
BssT1I CCWWGG 1 cut(s) 196
Bst4CI ACNGT 1 cut(s) 190
BstC8I GCNNGC 5 cut(s) 21, 182, 274, 412, 505
BstDEI CTNAG 1 cut(s) 125
BstF5I GGATG 2 cut(s) 302, 373
BstMWI GCNNNNNNNGC 1 cut(s) 411
BstNSI RCATGY 1 cut(s) 452
BstSCI CCNGG 1 cut(s) 27
BstSFI CTRYAG 1 cut(s) 280
BstV1I GCAGC 1 cut(s) 155
BsuI GTATCC 1 cut(s) 425
BsuRI GGCC 4 cut(s) 62, 276, 392, 398
BtsCI GGATG 2 cut(s) 302, 373
BtsI GCAGTG 2 cut(s) 405, 461
BtsIMutI CAGTG 3 cut(s) 399, 405, 461
Cac8I GCNNGC 5 cut(s) 21, 182, 274, 412, 505
Cfr10I RCCGGY 2 cut(s) 35, 410
Cfr13I GGNCC 1 cut(s) 200
Csp6I GTAC 1 cut(s) 487
CspAI ACCGGT 1 cut(s) 35
CviAII CATG 2 cut(s) 66, 449
CviQI GTAC 1 cut(s) 487
DdeI CTNAG 1 cut(s) 125
EaeI YGGCCR 1 cut(s) 396
Eco130I CCWWGG 1 cut(s) 196
Eco147I AGGCCT 2 cut(s) 62, 276
Eco47I GGWCC 1 cut(s) 200
EcoO109I RGGNCCY 1 cut(s) 200
EcoT14I CCWWGG 1 cut(s) 196
ErhI CCWWGG 1 cut(s) 196
FaeI CATG 2 cut(s) 69, 452
FatI CATG 2 cut(s) 65, 448
Fnu4HI GCNGC 1 cut(s) 144
FokI GGATG 2 cut(s) 289, 380
Fsp4HI GCNGC 1 cut(s) 144
FspBI CTAG 4 cut(s) 177, 299, 386, 542
GluI GCNGC 1 cut(s) 144
HaeIII GGCC 4 cut(s) 62, 276, 392, 398
HapII CCGG 3 cut(s) 29, 36, 411
Hin1II CATG 2 cut(s) 69, 452
HincII GTYRAC 2 cut(s) 100, 564
HindII GTYRAC 2 cut(s) 100, 564
HpaII CCGG 3 cut(s) 29, 36, 411
Hpy166II GTNNAC 2 cut(s) 100, 564
Hpy188I TCNGA 1 cut(s) 431
Hpy188III TCNNGA 1 cut(s) 319
Hpy8I GTNNAC 2 cut(s) 100, 564
Hpy99I CGWCG 2 cut(s) 13, 74
HpyAV CCTTC 2 cut(s) 250, 557
HpyCH4III ACNGT 1 cut(s) 190
HpyCH4V TGCA 7 cut(s) 110, 143, 272, 359, 422, 454, 492
HpyF10VI GCNNNNNNNGC 1 cut(s) 411
HpyF3I CTNAG 1 cut(s) 125
Hsp92II CATG 2 cut(s) 69, 452
KroI GCCGGC 1 cut(s) 410
KroNI GCCGGC 1 cut(s) 412
LmnI GCTCC 2 cut(s) 354, 512
Lsp1109I GCAGC 1 cut(s) 155
LweI GCATC 3 cut(s) 14, 155, 311
MaeI CTAG 4 cut(s) 177, 299, 386, 542
MaeIII GTNAC 1 cut(s) 172
MluCI AATT 1 cut(s) 585
MnlI CCTC 7 cut(s) 73, 90, 93, 96, 287, 403, 521
MroNI GCCGGC 1 cut(s) 410
MspA1I CMGCKG 1 cut(s) 23
MspI CCGG 3 cut(s) 29, 36, 411
MspR9I CCNGG 1 cut(s) 29
MwoI GCNNNNNNNGC 1 cut(s) 411
NaeI GCCGGC 1 cut(s) 412
NciI CCSGG 1 cut(s) 29
NgoMIV GCCGGC 1 cut(s) 410
NlaIII CATG 2 cut(s) 69, 452
NlaIV GGNNCC 1 cut(s) 508
NmeAIII GCCGAG 1 cut(s) 374
NspI RCATGY 1 cut(s) 452
PceI AGGCCT 2 cut(s) 62, 276
PciI ACATGT 1 cut(s) 448
PdiI GCCGGC 1 cut(s) 412
PinAI ACCGGT 1 cut(s) 35
PkrI GCNGC 1 cut(s) 145
PpuMI RGGWCCY 1 cut(s) 200
PscI ACATGT 1 cut(s) 448
Psp5II RGGWCCY 1 cut(s) 200
PspN4I GGNNCC 1 cut(s) 508
PspPI GGNCC 1 cut(s) 200
PspPPI RGGWCCY 1 cut(s) 200
PvuII CAGCTG 1 cut(s) 23
RsaI GTAC 1 cut(s) 488
RsaNI GTAC 1 cut(s) 487
SatI GCNGC 1 cut(s) 144
Sau96I GGNCC 1 cut(s) 200
ScaI AGTACT 1 cut(s) 488
ScrFI CCNGG 1 cut(s) 29
SetI ASST 4 cut(s) 25, 131, 202, 468
SfaNI GCATC 3 cut(s) 14, 155, 311
SfcI CTRYAG 1 cut(s) 280
SinI GGWCC 1 cut(s) 200
Sse9I AATT 1 cut(s) 585
SseBI AGGCCT 2 cut(s) 62, 276
SsiI CCGC 1 cut(s) 414
SspI AATATT 1 cut(s) 330
SspMI CTAG 4 cut(s) 177, 299, 386, 542
StuI AGGCCT 2 cut(s) 62, 276
StyD4I CCNGG 1 cut(s) 27
StyI CCWWGG 1 cut(s) 196
TaaI ACNGT 1 cut(s) 190
TaqI TCGA 1 cut(s) 8
TasI AATT 1 cut(s) 585
TatI WGTACW 1 cut(s) 486
TscAI CASTG 3 cut(s) 406, 412, 461
TseI GCWGC 1 cut(s) 143
TspDTI ATGAA 3 cut(s) 232, 459, 507
TspGWI ACGGA 1 cut(s) 26
TspRI CASTG 3 cut(s) 406, 412, 461
VpaK11BI GGWCC 1 cut(s) 200
XapI RAATTY 1 cut(s) 585
XceI RCATGY 1 cut(s) 452
XspI CTAG 4 cut(s) 177, 299, 386, 542
ZrmI AGTACT 1 cut(s) 488
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.