AT4G16210

enoyl-CoA hydratase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0012967
Physical Location & Seq
Reverse (-)
9176499 .. 9178139
1641 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G16210.1

Sequence Viewer

Length: 798 bp
ATGGATCAAACAGTATCGGAAAATCTCATTCAGGTGAAGAAAGAATCAGGCGGGATCGCCGTCATCACAATCAACCGTCCGAAATCTCTCAATTCACTCACGAGAGCGATGATGGTTGATCTCGCCAAGGCATTCAAGGATATGGACTCCGACGAATCGGTCCAGGTCGTGATTTTCACCGGATCAGGTCGATCTTTCTGCTCTGGCGTTGATTTGACTGCGGCGGAGTCTGTTTTCAAAGGAGACGTGAAGGATCCGGAAACCGACCCGGTTGTGCAGATGGAGCGGTTACGTAAACCGATCATCGGAGCTATTAACGGTTTTGCCATCACCGCCGGGTTTGAACTCGCCTTGGCCTGTGATATTTTGGTCGCTTCTAGAGGAGCTAAGTTCATGGATACTCACGCCAGGTTTGGGATATTTCCTTCATGGGGTTTGTCTCAGAAGCTGTCGAGGATCATCGGAGCAAACAAAGCTCGAGAAGTTTCTTTAACATCGATGCCACTGACAGCAGACGTGGCTGGGAAGTTAGGGTTTGTAAACCATGTGGTTGAAGAAGGAGAAGCGTTAAAGAAAGCTAGAGAAATCGCAGAGGCTATAATCAAGAATGAACAAGGCATGGTTCTGAGGATTAAATCCGTTATCAATGATGGACTTAAGCTTGATCTTGGGCATGCTCTCACACTAGAAAAGGAGCGAGCTCACGCGTATTACAGTGGGATGACGAAGGAACAGTTTAGAAAGATGCAGGAGTTTATAGCCGGACGTGGATCCAAGAAACCTTCTTCTAAGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ECH_1

Protein Analysis

265

Amino Acids

28.82

Weight (kDa)

9.11

Isoelectric Point (pI)

23.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ECH_1 PF00378 15 - 239 4.3e-50 Enoyl-CoA hydratase/isomerase
ECH_2 PF16113 20 - 199 2.1e-24 Enoyl-CoA hydratase/isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012967)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G16210
fragaria_vesca FvH4_4g19880
malus_domestica MD16G1152800.v1.1
prunus_persica Prupe.1G116000_v2.0.a1
pyrus_communis pycom16g12990
rosa_chinensis RchiOBHm_Chr4g0425501 RchiOBHm_Chr4g0425511
rosa_laevigata RLG00000007359 RLG00000007363 RLG00000007365 RLG00000007367
rosa_multiflora Rmu_sc0001900.1_g000011
rosa_roxburghii Rroxscaffold_5G00367450
rosa_rugosa Rorug04G0203600
rosa_samantha Rh4AG260600 Rh4BG266300 Rh4DG262600
rosa_wichuraiana Rw4G022560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 158
AccBSI CCGCTC 1 cut(s) 286
AccII CGCG 1 cut(s) 707
AccIII TCCGGA 1 cut(s) 256
AciI CCGC 5 cut(s) 51, 221, 224, 286, 333
AclWI GGATC 8 cut(s) 12, 62, 190, 248, 261, 464, 765, 778
AfiI CCNNNNNNNGG 3 cut(s) 305, 414, 431
AflII CTTAAG 1 cut(s) 656
AflIII ACRYGT 1 cut(s) 705
AgsI TTSAA 4 cut(s) 136, 238, 344, 554
AjiI CACGTC 3 cut(s) 247, 517, 767
AjnI CCWGG 2 cut(s) 162, 407
AluBI AGCT 7 cut(s) 311, 386, 448, 476, 578, 661, 701
AluI AGCT 7 cut(s) 311, 386, 448, 476, 578, 661, 701
Alw21I GWGCWC 1 cut(s) 703
Alw26I GTCTC 2 cut(s) 237, 444
AlwI GGATC 8 cut(s) 12, 62, 190, 248, 261, 464, 765, 778
AlwNI CAGNNNCTG 1 cut(s) 448
Ama87I CYCGRG 1 cut(s) 477
Aor13HI TCCGGA 1 cut(s) 256
AoxI GGCC 1 cut(s) 354
AspS9I GGNCC 1 cut(s) 160
AsuC2I CCSGG 2 cut(s) 269, 337
AsuHPI GGTGA 3 cut(s) 46, 169, 322
AvaI CYCGRG 1 cut(s) 477
AvaII GGWCC 1 cut(s) 160
BamHI GGATCC 2 cut(s) 253, 770
BanII GRGCYC 1 cut(s) 703
BauI CACGAG 1 cut(s) 100
Bbv12I GWGCWC 1 cut(s) 703
BccI CCATC 4 cut(s) 106, 274, 335, 644
BceAI ACGGC 1 cut(s) 44
BcgI CGANNNNNNTGC 2 cut(s) 180, 214
BciT130I CCWGG 2 cut(s) 164, 409
BciVI GTATCC 1 cut(s) 391
BcnI CCSGG 2 cut(s) 269, 337
BcoDI GTCTC 2 cut(s) 237, 444
BfaI CTAG 3 cut(s) 378, 579, 686
BfrI CTTAAG 1 cut(s) 656
BfuI GTATCC 1 cut(s) 391
BisI GCNGC 1 cut(s) 222
BlsI GCNGC 1 cut(s) 223
Bme1390I CCNGG 4 cut(s) 164, 269, 337, 409
Bme18I GGWCC 1 cut(s) 160
BmeT110I CYCGRG 1 cut(s) 477
BmgBI CACGTC 3 cut(s) 247, 517, 767
BmgT120I GGNCC 1 cut(s) 160
BmiI GGNNCC 2 cut(s) 255, 772
BmrFI CCNGG 4 cut(s) 164, 269, 337, 409
BmsI GCATC 2 cut(s) 489, 735
BpuMI CCSGG 2 cut(s) 269, 337
Bsa29I ATCGAT 1 cut(s) 497
BsaAI YACGTR 1 cut(s) 293
BsaJI CCNNGG 2 cut(s) 126, 351
BsaWI WCCGGW 2 cut(s) 179, 256
Bsc4I CCNNNNNNNGG 3 cut(s) 305, 414, 431
BseAI TCCGGA 1 cut(s) 256
BseBI CCWGG 2 cut(s) 164, 409
BseCI ATCGAT 1 cut(s) 497
BseDI CCNNGG 2 cut(s) 126, 351
BseGI GGATG 1 cut(s) 726
BseLI CCNNNNNNNGG 3 cut(s) 305, 414, 431
BseMII CTCAG 2 cut(s) 455, 617
BseRI GAGGAG 1 cut(s) 396
BseYI CCCAGC 1 cut(s) 521
BsgI GTGCAG 1 cut(s) 296
Bsh1236I CGCG 1 cut(s) 707
BshFI GGCC 1 cut(s) 356
BshVI ATCGAT 1 cut(s) 497
BsiHKAI GWGCWC 1 cut(s) 703
BsiHKCI CYCGRG 1 cut(s) 477
BsiSI CCGG 5 cut(s) 180, 257, 269, 336, 762
BslI CCNNNNNNNGG 3 cut(s) 305, 414, 431
BsmAI GTCTC 2 cut(s) 237, 444
BsmBI CGTCTC 1 cut(s) 237
BsmI GAATGC 1 cut(s) 131
BsnI GGCC 1 cut(s) 356
BsoBI CYCGRG 1 cut(s) 477
Bsp1286I GDGCHC 1 cut(s) 703
Bsp13I TCCGGA 1 cut(s) 256
BspACI CCGC 5 cut(s) 51, 221, 224, 286, 333
BspANI GGCC 1 cut(s) 356
BspCNI CTCAG 2 cut(s) 454, 618
BspDI ATCGAT 1 cut(s) 497
BspEI TCCGGA 1 cut(s) 256
BspFNI CGCG 1 cut(s) 707
BspLI GGNNCC 2 cut(s) 255, 772
BspPI GGATC 8 cut(s) 12, 62, 190, 248, 261, 464, 765, 778
BspTI CTTAAG 1 cut(s) 656
BsrBI CCGCTC 1 cut(s) 286
BssECI CCNNGG 2 cut(s) 126, 351
BssSI CACGAG 1 cut(s) 100
BssT1I CCWWGG 2 cut(s) 126, 351
Bst2BI CACGAG 1 cut(s) 100
Bst2UI CCWGG 2 cut(s) 164, 409
Bst4CI ACNGT 5 cut(s) 13, 77, 320, 716, 735
BstAFI CTTAAG 1 cut(s) 656
BstBAI YACGTR 1 cut(s) 293
BstC8I GCNNGC 2 cut(s) 675, 699
BstDEI CTNAG 4 cut(s) 387, 441, 626, 789
BstF5I GGATG 1 cut(s) 726
BstFNI CGCG 1 cut(s) 707
BstMAI GTCTC 2 cut(s) 237, 444
BstMWI GCNNNNNNNGC 4 cut(s) 283, 332, 473, 518
BstNI CCWGG 2 cut(s) 164, 409
BstNSI RCATGY 1 cut(s) 677
BstSCI CCNGG 4 cut(s) 162, 267, 335, 407
BstSNI TACGTA 1 cut(s) 293
BstUI CGCG 1 cut(s) 707
BstX2I RGATCY 2 cut(s) 253, 770
BstYI RGATCY 2 cut(s) 253, 770
Bsu15I ATCGAT 1 cut(s) 497
BsuI GTATCC 1 cut(s) 391
BsuRI GGCC 1 cut(s) 356
BsuTUI ATCGAT 1 cut(s) 497
BtgZI GCGATG 1 cut(s) 122
BtrI CACGTC 3 cut(s) 247, 517, 767
BtsCI GGATG 1 cut(s) 726
BtsIMutI CAGTG 2 cut(s) 503, 721
Cac8I GCNNGC 2 cut(s) 675, 699
CaiI CAGNNNCTG 1 cut(s) 448
Cfr13I GGNCC 1 cut(s) 160
ClaI ATCGAT 1 cut(s) 497
CviAII CATG 5 cut(s) 394, 429, 545, 619, 674
DdeI CTNAG 4 cut(s) 387, 441, 626, 789
DrdI GACNNNNNNGTC 1 cut(s) 158
DseDI GACNNNNNNGTC 1 cut(s) 158
EciI GGCGGA 1 cut(s) 239
Ecl136II GAGCTC 1 cut(s) 701
Eco105I TACGTA 1 cut(s) 293
Eco130I CCWWGG 2 cut(s) 126, 351
Eco24I GRGCYC 1 cut(s) 703
Eco47I GGWCC 1 cut(s) 160
Eco53kI GAGCTC 1 cut(s) 701
Eco88I CYCGRG 1 cut(s) 477
EcoICRI GAGCTC 1 cut(s) 701
EcoRII CCWGG 2 cut(s) 162, 407
EcoT14I CCWWGG 2 cut(s) 126, 351
EcoT38I GRGCYC 1 cut(s) 703
ErhI CCWWGG 2 cut(s) 126, 351
Esp3I CGTCTC 1 cut(s) 237
FaeI CATG 5 cut(s) 397, 432, 548, 622, 677
FaiI YATR 8 cut(s) 143, 395, 430, 546, 599, 620, 675, 758
FatI CATG 5 cut(s) 393, 428, 544, 618, 673
FauI CCCGC 1 cut(s) 44
Fnu4HI GCNGC 1 cut(s) 222
FokI GGATG 1 cut(s) 733
FriOI GRGCYC 1 cut(s) 703
Fsp4HI GCNGC 1 cut(s) 222
FspBI CTAG 3 cut(s) 378, 579, 686
GluI GCNGC 1 cut(s) 222
GsaI CCCAGC 1 cut(s) 525
HaeIII GGCC 1 cut(s) 356
HapII CCGG 5 cut(s) 180, 257, 269, 336, 762
Hin1II CATG 5 cut(s) 397, 432, 548, 622, 677
HindIII AAGCTT 1 cut(s) 659
HinfI GANTC 4 cut(s) 44, 146, 155, 227
HpaII CCGG 5 cut(s) 180, 257, 269, 336, 762
HphI GGTGA 3 cut(s) 46, 169, 322
Hpy166II GTNNAC 2 cut(s) 296, 541
Hpy188I TCNGA 7 cut(s) 19, 81, 151, 308, 444, 464, 627
Hpy188III TCNNGA 6 cut(s) 100, 169, 257, 378, 479, 604
Hpy8I GTNNAC 2 cut(s) 296, 541
Hpy99I CGWCG 1 cut(s) 155
HpyAV CCTTC 5 cut(s) 244, 435, 551, 721, 792
HpyCH4III ACNGT 5 cut(s) 13, 77, 320, 716, 735
HpyCH4IV ACGT 4 cut(s) 246, 292, 516, 766
HpyCH4V TGCA 2 cut(s) 277, 748
HpyF10VI GCNNNNNNNGC 4 cut(s) 283, 332, 473, 518
HpyF3I CTNAG 4 cut(s) 387, 441, 626, 789
HpySE526I ACGT 4 cut(s) 246, 292, 516, 766
Hsp92II CATG 5 cut(s) 397, 432, 548, 622, 677
Kpn2I TCCGGA 1 cut(s) 256
LmnI GCTCC 5 cut(s) 283, 308, 383, 464, 694
LweI GCATC 2 cut(s) 489, 735
MaeI CTAG 3 cut(s) 378, 579, 686
MaeII ACGT 4 cut(s) 246, 292, 516, 766
MaeIII GTNAC 1 cut(s) 288
MbiI CCGCTC 1 cut(s) 286
MboII GAAGA 3 cut(s) 49, 566, 777
MflI RGATCY 2 cut(s) 253, 770
MhlI GDGCHC 1 cut(s) 703
MluCI AATT 1 cut(s) 91
MluI ACGCGT 1 cut(s) 705
MlyI GAGTC 2 cut(s) 140, 236
MmeI TCCRAC 1 cut(s) 174
MnlI CCTC 4 cut(s) 374, 447, 586, 621
MroI TCCGGA 1 cut(s) 256
MseI TTAA 5 cut(s) 315, 491, 569, 633, 657
MslI CAYNNNNRTG 1 cut(s) 32
MspCI CTTAAG 1 cut(s) 656
MspI CCGG 5 cut(s) 180, 257, 269, 336, 762
MspR9I CCNGG 4 cut(s) 164, 269, 337, 409
Mva1269I GAATGC 1 cut(s) 131
MvaI CCWGG 2 cut(s) 164, 409
MvnI CGCG 1 cut(s) 707
MwoI GCNNNNNNNGC 4 cut(s) 283, 332, 473, 518
NciI CCSGG 2 cut(s) 269, 337
NlaIII CATG 5 cut(s) 397, 432, 548, 622, 677
NlaIV GGNNCC 2 cut(s) 255, 772
NspI RCATGY 1 cut(s) 677
PaeI GCATGC 1 cut(s) 677
PaeR7I CTCGAG 1 cut(s) 477
PctI GAATGC 1 cut(s) 131
PfeI GAWTC 2 cut(s) 44, 155
PkrI GCNGC 1 cut(s) 223
PleI GAGTC 2 cut(s) 140, 235
PpsI GAGTC 2 cut(s) 140, 235
Ppu21I YACGTR 1 cut(s) 293
Psp124BI GAGCTC 1 cut(s) 703
Psp6I CCWGG 2 cut(s) 162, 407
PspFI CCCAGC 1 cut(s) 521
PspGI CCWGG 2 cut(s) 162, 407
PspN4I GGNNCC 2 cut(s) 255, 772
PspPI GGNCC 1 cut(s) 160
PstNI CAGNNNCTG 1 cut(s) 448
PsuI RGATCY 2 cut(s) 253, 770
RseI CAYNNNNRTG 1 cut(s) 32
SacI GAGCTC 1 cut(s) 703
SaqAI TTAA 5 cut(s) 315, 491, 569, 633, 657
SatI GCNGC 1 cut(s) 222
Sau96I GGNCC 1 cut(s) 160
SchI GAGTC 2 cut(s) 140, 236
ScrFI CCNGG 4 cut(s) 164, 269, 337, 409
SduI GDGCHC 1 cut(s) 703
SfaNI GCATC 2 cut(s) 489, 735
Sfr274I CTCGAG 1 cut(s) 477
SinI GGWCC 1 cut(s) 160
SlaI CTCGAG 1 cut(s) 477
SmiMI CAYNNNNRTG 1 cut(s) 32
SmlI CTYRAG 2 cut(s) 477, 656
SmoI CTYRAG 2 cut(s) 477, 656
SnaBI TACGTA 1 cut(s) 293
SphI GCATGC 1 cut(s) 677
Sse9I AATT 1 cut(s) 91
SsiI CCGC 5 cut(s) 51, 221, 224, 286, 333
SspMI CTAG 3 cut(s) 378, 579, 686
SstI GAGCTC 1 cut(s) 703
StyD4I CCNGG 4 cut(s) 162, 267, 335, 407
StyI CCWWGG 2 cut(s) 126, 351
TaaI ACNGT 5 cut(s) 13, 77, 320, 716, 735
TaiI ACGT 4 cut(s) 249, 295, 519, 769
TaqI TCGA 4 cut(s) 190, 452, 478, 497
TaqII GACCGA 1 cut(s) 148
TasI AATT 1 cut(s) 91
TauI GCSGC 1 cut(s) 224
TfiI GAWTC 2 cut(s) 44, 155
Tru1I TTAA 5 cut(s) 315, 491, 569, 633, 657
Tru9I TTAA 5 cut(s) 315, 491, 569, 633, 657
TscAI CASTG 2 cut(s) 510, 721
TspDTI ATGAA 3 cut(s) 382, 417, 624
TspGWI ACGGA 1 cut(s) 628
TspRI CASTG 2 cut(s) 510, 721
Vha464I CTTAAG 1 cut(s) 656
VpaK11BI GGWCC 1 cut(s) 160
XbaI TCTAGA 1 cut(s) 377
XceI RCATGY 1 cut(s) 677
XhoI CTCGAG 1 cut(s) 477
XspI CTAG 3 cut(s) 378, 579, 686
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.