RchiOBHm_Chr4g0425511

enoyl-CoA hydratase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0012967
Physical Location & Seq
Forward (+)
51126279 .. 51127731
1453 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ39466

Sequence Viewer

Length: 783 bp
ATGGACGGGAAACAATCTACGCCGGAAAATCTCATTCTCGTCAACCGCGAACCGGACGGAATCGCCTTCGTCAGGATCAACCGTCCCAAGTCGCTTAACTGGCTGACCAAGGCTATGATCATCGATCTCGCCCGGGCCATCAAGTCCCTGAATCAAGACGATTCGGTCCGGGTCATTATTCTTTCGGGTACGGGTCGGGCCTTCTGCTCCGGCGTCGATCTCACCGTGGCCGAGGACGTTTTCAAAGGCGACGTCAAGGACATCGAGACCGCGGGCCCCCAAATAGAGCTATACAAAAAGCCCATCATCGCCGCCATCCACGGCTTCGCGGTCGCAGGCGGATTCGAAATCGCGATCGCTTGCCCGAATCCGAATTTTGTTGACACTTTCGAGGATAATAGGGGCAACAAAGCGCGTGAGGTGTCACTGACGGCGACGCCGCTAACGGTGGAGGTGGGTGAGAGGTTGGGGTTAGTGAACCATGTTGTGGAGGAGAGTGAGTTGCTGAAGAAAGGGCGGGAAATAGCAGAGGCCATTGTGAAGAATAACCAGGACCTGGAGGTGAGGTACAAGGCTGTTATTAATGATGGGCTTAAGTTGGACCTGGGTACGTCATGCTCTTACACTAGAAAAGGTCTGCAATTTCGACTTAATTTTGTGGTTTTTGATGTTGCGTTGGAGAGGGCTCGTGAATATTACAATGGAATGACCAAGGATCAGTTTAAGAAGATGCAAGAGTTTATAGCTGGTCGGAGTTCGAAAAAGCCTTCTTCCAAGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ECH_1

Protein Analysis

260

Amino Acids

28.8

Weight (kDa)

8.32

Isoelectric Point (pI)

28.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ECH_1 PF00378 18 - 121 9.3e-22 Enoyl-CoA hydratase/isomerase
ECH_2 PF16113 22 - 120 4e-16 Enoyl-CoA hydratase/isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012967)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G16210
fragaria_vesca FvH4_4g19880
malus_domestica MD16G1152800.v1.1
prunus_persica Prupe.1G116000_v2.0.a1
pyrus_communis pycom16g12990
rosa_chinensis RchiOBHm_Chr4g0425501 RchiOBHm_Chr4g0425511
rosa_laevigata RLG00000007359 RLG00000007363 RLG00000007365 RLG00000007367
rosa_multiflora Rmu_sc0001900.1_g000011
rosa_roxburghii Rroxscaffold_5G00367450
rosa_rugosa Rorug04G0203600
rosa_samantha Rh4AG260600 Rh4BG266300 Rh4DG262600
rosa_wichuraiana Rw4G022560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 164
AatII GACGTC 1 cut(s) 255
AccB7I CCANNNNNTGG 2 cut(s) 487, 556
AccII CGCG 5 cut(s) 48, 272, 329, 353, 415
AciI CCGC 8 cut(s) 46, 270, 272, 312, 329, 339, 440, 517
AclWI GGATC 2 cut(s) 83, 723
AcoI YGGCCR 1 cut(s) 228
AcsI RAATTY 1 cut(s) 373
AcuI CTGAAG 1 cut(s) 527
AcyI GRCGYC 3 cut(s) 213, 252, 437
AfaI GTAC 3 cut(s) 190, 569, 610
AfiI CCNNNNNNNGG 4 cut(s) 52, 72, 487, 556
AflII CTTAAG 1 cut(s) 593
AgsI TTSAA 1 cut(s) 244
AjnI CCWGG 3 cut(s) 549, 555, 603
AluBI AGCT 2 cut(s) 289, 746
AluI AGCT 2 cut(s) 289, 746
Alw26I GTCTC 1 cut(s) 260
AlwI GGATC 2 cut(s) 83, 723
AlwNI CAGNNNCTG 1 cut(s) 556
Ama87I CYCGRG 1 cut(s) 132
AoxI GGCC 5 cut(s) 135, 198, 228, 274, 531
ApaI GGGCCC 1 cut(s) 278
ApoI RAATTY 1 cut(s) 373
ArsI GACNNNNNNTTYG 2 cut(s) 237, 269
AseI ATTAAT 1 cut(s) 582
AsiSI GCGATCGC 1 cut(s) 357
AspLEI GCGC 1 cut(s) 415
AspS9I GGNCC 7 cut(s) 135, 166, 198, 274, 275, 553, 601
AsuC2I CCSGG 3 cut(s) 133, 134, 170
AsuHPI GGTGA 3 cut(s) 214, 470, 574
AsuII TTCGAA 2 cut(s) 345, 758
AvaI CYCGRG 1 cut(s) 132
AvaII GGWCC 3 cut(s) 166, 553, 601
BaeGI GKGCMC 1 cut(s) 278
BanII GRGCYC 2 cut(s) 278, 688
BauI CACGAG 1 cut(s) 687
BccI CCATC 4 cut(s) 146, 311, 323, 581
BceAI ACGGC 2 cut(s) 337, 447
BciT130I CCWGG 3 cut(s) 551, 557, 605
BclI TGATCA 1 cut(s) 117
BcnI CCSGG 3 cut(s) 133, 134, 170
BcoDI GTCTC 1 cut(s) 260
BfaI CTAG 1 cut(s) 627
BfrI CTTAAG 1 cut(s) 593
BisI GCNGC 2 cut(s) 312, 440
BlsI GCNGC 2 cut(s) 313, 441
Bme1390I CCNGG 6 cut(s) 133, 134, 170, 551, 557, 605
Bme18I GGWCC 3 cut(s) 166, 553, 601
BmeT110I CYCGRG 1 cut(s) 132
BmgT120I GGNCC 7 cut(s) 135, 166, 198, 274, 275, 553, 601
BmiI GGNNCC 2 cut(s) 276, 277
BmrFI CCNGG 6 cut(s) 133, 134, 170, 551, 557, 605
BmsI GCATC 1 cut(s) 720
BpmI CTGGAG 1 cut(s) 578
Bpu14I TTCGAA 2 cut(s) 345, 758
BpuMI CCSGG 3 cut(s) 133, 134, 170
Bsa29I ATCGAT 1 cut(s) 123
BsaHI GRCGYC 3 cut(s) 213, 252, 437
BsaI GGTCTC 1 cut(s) 260
BsaJI CCNNGG 8 cut(s) 108, 132, 225, 231, 270, 319, 604, 711
BsaWI WCCGGW 1 cut(s) 52
BsaXI ACNNNNNCTCC 4 cut(s) 485, 515, 551, 581
Bsc4I CCNNNNNNNGG 4 cut(s) 52, 72, 487, 556
Bse1I ACTGG 1 cut(s) 104
BseBI CCWGG 3 cut(s) 551, 557, 605
BseCI ATCGAT 1 cut(s) 123
BseDI CCNNGG 8 cut(s) 108, 132, 225, 231, 270, 319, 604, 711
BseGI GGATG 1 cut(s) 315
BseLI CCNNNNNNNGG 4 cut(s) 52, 72, 487, 556
BseNI ACTGG 1 cut(s) 104
BseRI GAGGAG 1 cut(s) 506
BseSI GKGCMC 1 cut(s) 278
Bsh1236I CGCG 5 cut(s) 48, 272, 329, 353, 415
Bsh1285I CGRYCG 2 cut(s) 333, 357
BshFI GGCC 5 cut(s) 137, 200, 230, 276, 533
BshVI ATCGAT 1 cut(s) 123
BsiEI CGRYCG 2 cut(s) 333, 357
BsiHKCI CYCGRG 1 cut(s) 132
BsiSI CCGG 5 cut(s) 23, 53, 133, 169, 210
BslFI GGGAC 2 cut(s) 69, 130
BslI CCNNNNNNNGG 4 cut(s) 52, 72, 487, 556
BsmAI GTCTC 1 cut(s) 260
BsmFI GGGAC 2 cut(s) 69, 130
BsnI GGCC 5 cut(s) 137, 200, 230, 276, 533
Bso31I GGTCTC 1 cut(s) 260
BsoBI CYCGRG 1 cut(s) 132
Bsp119I TTCGAA 2 cut(s) 345, 758
Bsp120I GGGCCC 1 cut(s) 274
Bsp1286I GDGCHC 2 cut(s) 278, 688
Bsp143I GATC 6 cut(s) 75, 117, 124, 217, 354, 715
Bsp68I TCGCGA 1 cut(s) 353
BspACI CCGC 8 cut(s) 46, 270, 272, 312, 329, 339, 440, 517
BspANI GGCC 5 cut(s) 137, 200, 230, 276, 533
BspDI ATCGAT 1 cut(s) 123
BspFNI CGCG 5 cut(s) 48, 272, 329, 353, 415
BspLI GGNNCC 2 cut(s) 276, 277
BspPI GGATC 2 cut(s) 83, 723
BspT104I TTCGAA 2 cut(s) 345, 758
BspTI CTTAAG 1 cut(s) 593
BspTNI GGTCTC 1 cut(s) 260
BsrI ACTGG 1 cut(s) 104
BssECI CCNNGG 8 cut(s) 108, 132, 225, 231, 270, 319, 604, 711
BssMI GATC 6 cut(s) 75, 117, 124, 217, 354, 715
BssNI GRCGYC 3 cut(s) 213, 252, 437
BssSI CACGAG 1 cut(s) 687
BssT1I CCWWGG 2 cut(s) 108, 711
Bst2BI CACGAG 1 cut(s) 687
Bst2UI CCWGG 3 cut(s) 551, 557, 605
Bst4CI ACNGT 3 cut(s) 83, 226, 448
BstACI GRCGYC 3 cut(s) 213, 252, 437
BstAFI CTTAAG 1 cut(s) 593
BstBI TTCGAA 2 cut(s) 345, 758
BstC8I GCNNGC 3 cut(s) 274, 337, 361
BstDSI CCRYGG 3 cut(s) 225, 270, 319
BstENI CCTNNNNNAGG 1 cut(s) 70
BstF5I GGATG 1 cut(s) 315
BstFNI CGCG 5 cut(s) 48, 272, 329, 353, 415
BstHHI GCGC 1 cut(s) 415
BstKTI GATC 6 cut(s) 78, 120, 127, 220, 357, 718
BstMAI GTCTC 1 cut(s) 260
BstMBI GATC 6 cut(s) 75, 117, 124, 217, 354, 715
BstMCI CGRYCG 2 cut(s) 333, 357
BstMWI GCNNNNNNNGC 1 cut(s) 100
BstNI CCWGG 3 cut(s) 551, 557, 605
BstSCI CCNGG 6 cut(s) 131, 132, 168, 549, 555, 603
BstSLI GKGCMC 1 cut(s) 278
BstUI CGCG 5 cut(s) 48, 272, 329, 353, 415
Bsu15I ATCGAT 1 cut(s) 123
BsuRI GGCC 5 cut(s) 137, 200, 230, 276, 533
BsuTUI ATCGAT 1 cut(s) 123
BtgI CCRYGG 3 cut(s) 225, 270, 319
BtgZI GCGATG 1 cut(s) 292
BtsCI GGATG 1 cut(s) 315
BtsIMutI CAGTG 1 cut(s) 425
BtuMI TCGCGA 1 cut(s) 353
Cac8I GCNNGC 3 cut(s) 274, 337, 361
CaiI CAGNNNCTG 1 cut(s) 556
CfoI GCGC 1 cut(s) 415
Cfr13I GGNCC 7 cut(s) 135, 166, 198, 274, 275, 553, 601
Cfr42I CCGCGG 1 cut(s) 273
Cfr9I CCCGGG 1 cut(s) 132
ClaI ATCGAT 1 cut(s) 123
CpoI CGGWCCG 1 cut(s) 166
CseI GACGC 2 cut(s) 202, 445
Csp6I GTAC 3 cut(s) 189, 568, 609
CspI CGGWCCG 1 cut(s) 166
CviAII CATG 2 cut(s) 482, 615
CviQI GTAC 3 cut(s) 189, 568, 609
DpnI GATC 6 cut(s) 77, 119, 126, 219, 356, 717
DpnII GATC 6 cut(s) 75, 117, 124, 217, 354, 715
DrdI GACNNNNNNGTC 1 cut(s) 164
DseDI GACNNNNNNGTC 1 cut(s) 164
EaeI YGGCCR 1 cut(s) 228
EciI GGCGGA 1 cut(s) 354
Eco130I CCWWGG 2 cut(s) 108, 711
Eco24I GRGCYC 2 cut(s) 278, 688
Eco31I GGTCTC 1 cut(s) 260
Eco47I GGWCC 3 cut(s) 166, 553, 601
Eco57I CTGAAG 1 cut(s) 527
Eco88I CYCGRG 1 cut(s) 132
EcoNI CCTNNNNNAGG 1 cut(s) 70
EcoO109I RGGNCCY 2 cut(s) 275, 553
EcoRII CCWGG 3 cut(s) 549, 555, 603
EcoT14I CCWWGG 2 cut(s) 108, 711
EcoT38I GRGCYC 2 cut(s) 278, 688
ErhI CCWWGG 2 cut(s) 108, 711
FaeI CATG 2 cut(s) 485, 618
FaiI YATR 5 cut(s) 116, 292, 483, 616, 743
FaqI GGGAC 2 cut(s) 69, 130
FatI CATG 2 cut(s) 481, 614
FauI CCCGC 2 cut(s) 265, 510
FbaI TGATCA 1 cut(s) 117
Fnu4HI GCNGC 2 cut(s) 312, 440
FokI GGATG 1 cut(s) 302
FriOI GRGCYC 2 cut(s) 278, 688
Fsp4HI GCNGC 2 cut(s) 312, 440
FspBI CTAG 1 cut(s) 627
GlaI GCGC 1 cut(s) 414
GluI GCNGC 2 cut(s) 312, 440
GsuI CTGGAG 1 cut(s) 578
HaeIII GGCC 5 cut(s) 137, 200, 230, 276, 533
HapII CCGG 5 cut(s) 23, 53, 133, 169, 210
HgaI GACGC 2 cut(s) 202, 445
HhaI GCGC 1 cut(s) 415
Hin1I GRCGYC 3 cut(s) 213, 252, 437
Hin1II CATG 2 cut(s) 485, 618
Hin6I GCGC 1 cut(s) 413
HinP1I GCGC 1 cut(s) 413
HincII GTYRAC 2 cut(s) 43, 382
HindII GTYRAC 2 cut(s) 43, 382
HinfI GANTC 5 cut(s) 60, 151, 161, 342, 367
HpaII CCGG 5 cut(s) 23, 53, 133, 169, 210
HphI GGTGA 3 cut(s) 214, 470, 574
Hpy166II GTNNAC 3 cut(s) 43, 382, 478
Hpy188I TCNGA 2 cut(s) 372, 753
Hpy188III TCNNGA 5 cut(s) 73, 155, 265, 352, 689
Hpy8I GTNNAC 3 cut(s) 43, 382, 478
Hpy99I CGWCG 3 cut(s) 218, 254, 439
HpyAV CCTTC 3 cut(s) 76, 211, 777
HpyCH4III ACNGT 3 cut(s) 83, 226, 448
HpyCH4IV ACGT 3 cut(s) 237, 252, 611
HpyCH4V TGCA 2 cut(s) 640, 733
HpyF10VI GCNNNNNNNGC 1 cut(s) 100
HpySE526I ACGT 3 cut(s) 237, 252, 611
Hsp92I GRCGYC 3 cut(s) 213, 252, 437
Hsp92II CATG 2 cut(s) 485, 618
HspAI GCGC 1 cut(s) 413
Ksp22I TGATCA 1 cut(s) 117
KspI CCGCGG 1 cut(s) 273
Kzo9I GATC 6 cut(s) 75, 117, 124, 217, 354, 715
LmnI GCTCC 1 cut(s) 212
LweI GCATC 1 cut(s) 720
MaeI CTAG 1 cut(s) 627
MaeII ACGT 3 cut(s) 237, 252, 611
MaeIII GTNAC 1 cut(s) 423
MalI GATC 6 cut(s) 77, 119, 126, 219, 356, 717
MboI GATC 6 cut(s) 75, 117, 124, 217, 354, 715
MboII GAAGA 4 cut(s) 520, 553, 739, 762
MhlI GDGCHC 2 cut(s) 278, 688
MluCI AATT 3 cut(s) 373, 641, 652
MmeI TCCRAC 3 cut(s) 579, 657, 731
MseI TTAA 5 cut(s) 96, 582, 594, 651, 723
MspA1I CMGCKG 1 cut(s) 272
MspCI CTTAAG 1 cut(s) 593
MspI CCGG 5 cut(s) 23, 53, 133, 169, 210
MspR9I CCNGG 6 cut(s) 133, 134, 170, 551, 557, 605
MvaI CCWGG 3 cut(s) 551, 557, 605
MvnI CGCG 5 cut(s) 48, 272, 329, 353, 415
MwoI GCNNNNNNNGC 1 cut(s) 100
NciI CCSGG 3 cut(s) 133, 134, 170
NdeII GATC 6 cut(s) 75, 117, 124, 217, 354, 715
NlaIII CATG 2 cut(s) 485, 618
NlaIV GGNNCC 2 cut(s) 276, 277
NmeAIII GCCGAG 1 cut(s) 256
NmuCI GTSAC 1 cut(s) 423
NruI TCGCGA 1 cut(s) 353
NspV TTCGAA 2 cut(s) 345, 758
PcsI WCGNNNNNNNCGW 2 cut(s) 45, 222
PfeI GAWTC 5 cut(s) 60, 151, 161, 342, 367
PflMI CCANNNNNTGG 2 cut(s) 487, 556
PkrI GCNGC 2 cut(s) 313, 441
Ple19I CGATCG 1 cut(s) 357
PpuMI RGGWCCY 1 cut(s) 553
PshBI ATTAAT 1 cut(s) 582
Psp5II RGGWCCY 1 cut(s) 553
Psp6I CCWGG 3 cut(s) 549, 555, 603
PspGI CCWGG 3 cut(s) 549, 555, 603
PspN4I GGNNCC 2 cut(s) 276, 277
PspOMI GGGCCC 1 cut(s) 274
PspPI GGNCC 7 cut(s) 135, 166, 198, 274, 275, 553, 601
PspPPI RGGWCCY 1 cut(s) 553
PstNI CAGNNNCTG 1 cut(s) 556
PvuI CGATCG 1 cut(s) 357
RgaI GCGATCGC 1 cut(s) 357
RruI TCGCGA 1 cut(s) 353
RsaI GTAC 3 cut(s) 190, 569, 610
RsaNI GTAC 3 cut(s) 189, 568, 609
Rsr2I CGGWCCG 1 cut(s) 166
RsrII CGGWCCG 1 cut(s) 166
SacII CCGCGG 1 cut(s) 273
SaqAI TTAA 5 cut(s) 96, 582, 594, 651, 723
SatI GCNGC 2 cut(s) 312, 440
Sau3AI GATC 6 cut(s) 75, 117, 124, 217, 354, 715
Sau96I GGNCC 7 cut(s) 135, 166, 198, 274, 275, 553, 601
ScrFI CCNGG 6 cut(s) 133, 134, 170, 551, 557, 605
SduI GDGCHC 2 cut(s) 278, 688
SfaAI GCGATCGC 1 cut(s) 357
SfaNI GCATC 1 cut(s) 720
Sfr303I CCGCGG 1 cut(s) 273
SfuI TTCGAA 2 cut(s) 345, 758
SgfI GCGATCGC 1 cut(s) 357
SgrBI CCGCGG 1 cut(s) 273
SinI GGWCC 3 cut(s) 166, 553, 601
SmaI CCCGGG 1 cut(s) 134
SmlI CTYRAG 1 cut(s) 593
SmoI CTYRAG 1 cut(s) 593
SrfI GCCCGGGC 1 cut(s) 134
Sse9I AATT 3 cut(s) 373, 641, 652
SsiI CCGC 8 cut(s) 46, 270, 272, 312, 329, 339, 440, 517
SspI AATATT 1 cut(s) 695
SspMI CTAG 1 cut(s) 627
StyD4I CCNGG 6 cut(s) 131, 132, 168, 549, 555, 603
StyI CCWWGG 2 cut(s) 108, 711
TaaI ACNGT 3 cut(s) 83, 226, 448
TaiI ACGT 3 cut(s) 240, 255, 614
TaqI TCGA 7 cut(s) 123, 216, 264, 345, 390, 646, 758
TaqII GACCGA 1 cut(s) 154
TasI AATT 3 cut(s) 373, 641, 652
TauI GCSGC 2 cut(s) 314, 442
TfiI GAWTC 5 cut(s) 60, 151, 161, 342, 367
Tru1I TTAA 5 cut(s) 96, 582, 594, 651, 723
Tru9I TTAA 5 cut(s) 96, 582, 594, 651, 723
TscAI CASTG 1 cut(s) 432
TseFI GTSAC 1 cut(s) 423
Tsp45I GTSAC 1 cut(s) 423
TspGWI ACGGA 1 cut(s) 72
TspMI CCCGGG 1 cut(s) 132
TspRI CASTG 1 cut(s) 432
Van91I CCANNNNNTGG 2 cut(s) 487, 556
Vha464I CTTAAG 1 cut(s) 593
VpaK11BI GGWCC 3 cut(s) 166, 553, 601
VspI ATTAAT 1 cut(s) 582
XagI CCTNNNNNAGG 1 cut(s) 70
XapI RAATTY 1 cut(s) 373
XmaI CCCGGG 1 cut(s) 132
XspI CTAG 1 cut(s) 627
ZraI GACGTC 1 cut(s) 253
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.