FvH4_4g19880

enoyl-CoA hydratase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0012967
Physical Location & Seq
Forward (+)
23373722 .. 23376237
2516 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g19880.t2

Sequence Viewer

Length: 804 bp
ATGGACCGAAAACAATCCACGCCGGAAAATCCCATCCTCGTGAACCGCGAACCGGACGGGATCGCCTACGTCACCATCAACCGTCCAAAGCTGCTTAACTCCCTCACCAAGGCTATGATCACGGATCTCGCCCGGATCTTCAAGTCCCTAAACCAAGACGATTCGGTCCGGGTCATTATTCTCTCGGGTACGGGTCGGGCCTTCTGCTCCGGCGTCGATCTCACCGCCGCCGAGGACGTTTTCAAAGGCGACGTCAAGGACATCGAGACCGACCCGGTCGCCCAGATGGAGCGCTGCAAGAAGCCCATCATCGCCGCCGTCAACGGCTTCGCGGTCACCGCCGGATTCGAAATCGCGCTCGCCTGTGACATCGTCGTCGCCGCTAAGGGAGCCAAGTTCATCGACACTCATGCCAGATTTGGCATATTTCCGTCGTGGGGTTTGTCTCAGAAGCTTTCGAGAATTATAGGGGCGAACAAAGCGCGGGAGGTGTCGCTGACGGCGACGCCGTTGACGGCGGAGGTGGGGGAGAGGTTGGGGTTTGTGAACCATGTTGTGGGGGAGACTGAGTTGTTGAAGAAAGCGTGGGAAATAGCTGAGGCTATTGTGAAGAACAACCAGGACATGGTGGTGAGGTACAAGGCTGTTATTAATGATGGGGTCAAGCTGGACCTGGGCCATGCTCTTACATTAGAAAAGGAGAGGGCTCATGAATATTACAATGGAATGACCAAGGATCAGTTTAGGAAGATGCAAGAGTTTATAGCCGGTCGGAGTTCGAAAAAGCCTTCTTCCAAGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ECH_1

Protein Analysis

268

Amino Acids

29.35

Weight (kDa)

9.01

Isoelectric Point (pI)

22.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ECH_1 PF00378 18 - 233 2.7e-52 Enoyl-CoA hydratase/isomerase
ECH_2 PF16113 22 - 200 4.7e-25 Enoyl-CoA hydratase/isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012967)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G16210
fragaria_vesca FvH4_4g19880
malus_domestica MD16G1152800.v1.1
prunus_persica Prupe.1G116000_v2.0.a1
pyrus_communis pycom16g12990
rosa_chinensis RchiOBHm_Chr4g0425501 RchiOBHm_Chr4g0425511
rosa_laevigata RLG00000007359 RLG00000007363 RLG00000007365 RLG00000007367
rosa_multiflora Rmu_sc0001900.1_g000011
rosa_roxburghii Rroxscaffold_5G00367450
rosa_rugosa Rorug04G0203600
rosa_samantha Rh4AG260600 Rh4BG266300 Rh4DG262600
rosa_wichuraiana Rw4G022560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 164, 374
AatII GACGTC 1 cut(s) 255
AccB7I CCANNNNNTGG 2 cut(s) 556, 625
AccII CGCG 4 cut(s) 48, 332, 356, 484
AciI CCGC 9 cut(s) 46, 225, 228, 315, 332, 339, 381, 484, 518
AclWI GGATC 4 cut(s) 68, 132, 143, 744
AcyI GRCGYC 3 cut(s) 213, 252, 506
AfaI GTAC 2 cut(s) 190, 638
AfeI AGCGCT 1 cut(s) 293
AfiI CCNNNNNNNGG 5 cut(s) 52, 109, 437, 556, 625
AgsI TTSAA 3 cut(s) 142, 244, 577
AjnI CCWGG 2 cut(s) 618, 672
AluBI AGCT 4 cut(s) 91, 454, 596, 667
AluI AGCT 4 cut(s) 91, 454, 596, 667
Alw26I GTCTC 3 cut(s) 260, 450, 557
AlwI GGATC 4 cut(s) 68, 132, 143, 744
Ama87I CYCGRG 1 cut(s) 184
Aor51HI AGCGCT 1 cut(s) 293
AoxI GGCC 2 cut(s) 198, 676
ApeKI GCWGC 2 cut(s) 91, 294
ArsI GACNNNNNNTTYG 2 cut(s) 237, 269
AseI ATTAAT 1 cut(s) 651
AspLEI GCGC 3 cut(s) 294, 358, 484
AspS9I GGNCC 5 cut(s) 4, 166, 198, 670, 676
AsuC2I CCSGG 3 cut(s) 133, 170, 275
AsuHPI GGTGA 5 cut(s) 64, 97, 214, 328, 643
AsuII TTCGAA 2 cut(s) 348, 779
AvaI CYCGRG 1 cut(s) 184
AvaII GGWCC 3 cut(s) 4, 166, 670
BanII GRGCYC 1 cut(s) 709
BauI CACGAG 1 cut(s) 38
BbvCI CCTCAGC 1 cut(s) 597
BbvI GCAGC 2 cut(s) 78, 281
BccI CCATC 5 cut(s) 41, 83, 280, 314, 650
BceAI ACGGC 5 cut(s) 302, 340, 493, 516, 531
BcgI CGANNNNNNTGC 2 cut(s) 392, 426
BciT130I CCWGG 2 cut(s) 620, 674
BclI TGATCA 1 cut(s) 117
BcnI CCSGG 3 cut(s) 133, 170, 275
BcoDI GTCTC 3 cut(s) 260, 450, 557
BfoI RGCGCY 1 cut(s) 295
BisI GCNGC 5 cut(s) 92, 228, 295, 315, 381
BlsI GCNGC 5 cut(s) 93, 229, 296, 316, 382
Bme1390I CCNGG 5 cut(s) 133, 170, 275, 620, 674
Bme18I GGWCC 3 cut(s) 4, 166, 670
BmeT110I CYCGRG 1 cut(s) 184
BmgT120I GGNCC 5 cut(s) 4, 166, 198, 670, 676
BmiI GGNNCC 1 cut(s) 391
BmrFI CCNGG 5 cut(s) 133, 170, 275, 620, 674
BmsI GCATC 1 cut(s) 741
Bpu10I CCTNAGC 2 cut(s) 384, 597
Bpu14I TTCGAA 2 cut(s) 348, 779
BpuMI CCSGG 3 cut(s) 133, 170, 275
BsaHI GRCGYC 3 cut(s) 213, 252, 506
BsaI GGTCTC 1 cut(s) 260
BsaJI CCNNGG 4 cut(s) 108, 231, 673, 732
BsaWI WCCGGW 1 cut(s) 52
BsaXI ACNNNNNCTCC 2 cut(s) 554, 584
Bsc4I CCNNNNNNNGG 5 cut(s) 52, 109, 437, 556, 625
Bse118I RCCGGY 1 cut(s) 767
BseBI CCWGG 2 cut(s) 620, 674
BseDI CCNNGG 4 cut(s) 108, 231, 673, 732
BseGI GGATG 1 cut(s) 33
BseLI CCNNNNNNNGG 5 cut(s) 52, 109, 437, 556, 625
BseMII CTCAG 3 cut(s) 461, 558, 588
BseXI GCAGC 2 cut(s) 78, 281
Bsh1236I CGCG 4 cut(s) 48, 332, 356, 484
Bsh1285I CGRYCG 2 cut(s) 279, 772
BshFI GGCC 2 cut(s) 200, 678
BsiEI CGRYCG 2 cut(s) 279, 772
BsiHKCI CYCGRG 1 cut(s) 184
BsiSI CCGG 8 cut(s) 23, 53, 133, 169, 210, 275, 342, 768
BslFI GGGAC 1 cut(s) 130
BslI CCNNNNNNNGG 5 cut(s) 52, 109, 437, 556, 625
BsmAI GTCTC 3 cut(s) 260, 450, 557
BsmFI GGGAC 1 cut(s) 130
BsnI GGCC 2 cut(s) 200, 678
Bso31I GGTCTC 1 cut(s) 260
BsoBI CYCGRG 1 cut(s) 184
Bsp119I TTCGAA 2 cut(s) 348, 779
Bsp1286I GDGCHC 1 cut(s) 709
Bsp143I GATC 6 cut(s) 60, 117, 124, 135, 217, 736
BspACI CCGC 9 cut(s) 46, 225, 228, 315, 332, 339, 381, 484, 518
BspANI GGCC 2 cut(s) 200, 678
BspCNI CTCAG 3 cut(s) 460, 559, 589
BspFNI CGCG 4 cut(s) 48, 332, 356, 484
BspHI TCATGA 1 cut(s) 709
BspLI GGNNCC 1 cut(s) 391
BspPI GGATC 4 cut(s) 68, 132, 143, 744
BspT104I TTCGAA 2 cut(s) 348, 779
BspTNI GGTCTC 1 cut(s) 260
BsrFI RCCGGY 1 cut(s) 767
BssAI RCCGGY 1 cut(s) 767
BssECI CCNNGG 4 cut(s) 108, 231, 673, 732
BssMI GATC 6 cut(s) 60, 117, 124, 135, 217, 736
BssNI GRCGYC 3 cut(s) 213, 252, 506
BssSI CACGAG 1 cut(s) 38
BssT1I CCWWGG 2 cut(s) 108, 732
Bst2BI CACGAG 1 cut(s) 38
Bst2UI CCWGG 2 cut(s) 620, 674
Bst4CI ACNGT 1 cut(s) 83
BstACI GRCGYC 3 cut(s) 213, 252, 506
BstBI TTCGAA 2 cut(s) 348, 779
BstC8I GCNNGC 1 cut(s) 360
BstDEI CTNAG 4 cut(s) 384, 447, 567, 597
BstEII GGTNACC 1 cut(s) 334
BstENI CCTNNNNNAGG 1 cut(s) 107
BstF5I GGATG 1 cut(s) 33
BstFNI CGCG 4 cut(s) 48, 332, 356, 484
BstH2I RGCGCY 1 cut(s) 295
BstHHI GCGC 3 cut(s) 294, 358, 484
BstKTI GATC 6 cut(s) 63, 120, 127, 138, 220, 739
BstMAI GTCTC 3 cut(s) 260, 450, 557
BstMBI GATC 6 cut(s) 60, 117, 124, 135, 217, 736
BstMCI CGRYCG 2 cut(s) 279, 772
BstMWI GCNNNNNNNGC 3 cut(s) 338, 389, 479
BstNI CCWGG 2 cut(s) 620, 674
BstPI GGTNACC 1 cut(s) 334
BstSCI CCNGG 5 cut(s) 131, 168, 273, 618, 672
BstUI CGCG 4 cut(s) 48, 332, 356, 484
BstV1I GCAGC 2 cut(s) 78, 281
BstX2I RGATCY 2 cut(s) 124, 135
BstYI RGATCY 2 cut(s) 124, 135
BsuRI GGCC 2 cut(s) 200, 678
BtgZI GCGATG 1 cut(s) 295
BtsCI GGATG 1 cut(s) 33
Cac8I GCNNGC 1 cut(s) 360
CciI TCATGA 1 cut(s) 709
CfoI GCGC 3 cut(s) 294, 358, 484
Cfr10I RCCGGY 1 cut(s) 767
Cfr13I GGNCC 5 cut(s) 4, 166, 198, 670, 676
CpoI CGGWCCG 1 cut(s) 166
CseI GACGC 2 cut(s) 202, 514
Csp6I GTAC 2 cut(s) 189, 637
CspI CGGWCCG 1 cut(s) 166
CviAII CATG 5 cut(s) 410, 551, 625, 680, 710
CviQI GTAC 2 cut(s) 189, 637
DdeI CTNAG 4 cut(s) 384, 447, 567, 597
DpnI GATC 6 cut(s) 62, 119, 126, 137, 219, 738
DpnII GATC 6 cut(s) 60, 117, 124, 135, 217, 736
DrdI GACNNNNNNGTC 2 cut(s) 164, 374
DseDI GACNNNNNNGTC 2 cut(s) 164, 374
EciI GGCGGA 1 cut(s) 533
Eco130I CCWWGG 2 cut(s) 108, 732
Eco24I GRGCYC 1 cut(s) 709
Eco31I GGTCTC 1 cut(s) 260
Eco47I GGWCC 3 cut(s) 4, 166, 670
Eco47III AGCGCT 1 cut(s) 293
Eco88I CYCGRG 1 cut(s) 184
Eco91I GGTNACC 1 cut(s) 334
EcoNI CCTNNNNNAGG 1 cut(s) 107
EcoO65I GGTNACC 1 cut(s) 334
EcoRII CCWGG 2 cut(s) 618, 672
EcoT14I CCWWGG 2 cut(s) 108, 732
EcoT38I GRGCYC 1 cut(s) 709
ErhI CCWWGG 2 cut(s) 108, 732
FaeI CATG 5 cut(s) 413, 554, 628, 683, 713
FaiI YATR 9 cut(s) 116, 411, 425, 467, 552, 626, 681, 711, 764
FaqI GGGAC 1 cut(s) 130
FatI CATG 5 cut(s) 409, 550, 624, 679, 709
FauI CCCGC 1 cut(s) 477
FbaI TGATCA 1 cut(s) 117
Fnu4HI GCNGC 5 cut(s) 92, 228, 295, 315, 381
FokI GGATG 1 cut(s) 20
FriOI GRGCYC 1 cut(s) 709
Fsp4HI GCNGC 5 cut(s) 92, 228, 295, 315, 381
GlaI GCGC 3 cut(s) 293, 357, 483
GluI GCNGC 5 cut(s) 92, 228, 295, 315, 381
HaeII RGCGCY 1 cut(s) 295
HaeIII GGCC 2 cut(s) 200, 678
HapII CCGG 8 cut(s) 23, 53, 133, 169, 210, 275, 342, 768
HgaI GACGC 2 cut(s) 202, 514
HhaI GCGC 3 cut(s) 294, 358, 484
Hin1I GRCGYC 3 cut(s) 213, 252, 506
Hin1II CATG 5 cut(s) 413, 554, 628, 683, 713
Hin6I GCGC 3 cut(s) 292, 356, 482
HinP1I GCGC 3 cut(s) 292, 356, 482
HincII GTYRAC 2 cut(s) 322, 513
HindII GTYRAC 2 cut(s) 322, 513
HindIII AAGCTT 1 cut(s) 452
HinfI GANTC 2 cut(s) 161, 345
HpaII CCGG 8 cut(s) 23, 53, 133, 169, 210, 275, 342, 768
HphI GGTGA 5 cut(s) 64, 97, 214, 328, 643
Hpy166II GTNNAC 4 cut(s) 43, 322, 513, 547
Hpy188I TCNGA 2 cut(s) 450, 774
Hpy188III TCNNGA 4 cut(s) 40, 265, 459, 710
Hpy8I GTNNAC 4 cut(s) 43, 322, 513, 547
Hpy99I CGWCG 6 cut(s) 218, 254, 377, 380, 436, 508
HpyAV CCTTC 2 cut(s) 211, 798
HpyCH4III ACNGT 1 cut(s) 83
HpyCH4IV ACGT 3 cut(s) 69, 237, 252
HpyCH4V TGCA 2 cut(s) 297, 754
HpyF10VI GCNNNNNNNGC 3 cut(s) 338, 389, 479
HpyF3I CTNAG 4 cut(s) 384, 447, 567, 597
HpySE526I ACGT 3 cut(s) 69, 237, 252
Hsp92I GRCGYC 3 cut(s) 213, 252, 506
Hsp92II CATG 5 cut(s) 413, 554, 628, 683, 713
HspAI GCGC 3 cut(s) 292, 356, 482
Ksp22I TGATCA 1 cut(s) 117
Kzo9I GATC 6 cut(s) 60, 117, 124, 135, 217, 736
LmnI GCTCC 3 cut(s) 212, 289, 389
Lsp1109I GCAGC 2 cut(s) 78, 281
LweI GCATC 1 cut(s) 741
MaeII ACGT 3 cut(s) 69, 237, 252
MaeIII GTNAC 3 cut(s) 70, 334, 365
MalI GATC 6 cut(s) 62, 119, 126, 137, 219, 738
MboI GATC 6 cut(s) 60, 117, 124, 135, 217, 736
MboII GAAGA 5 cut(s) 130, 589, 622, 760, 783
MflI RGATCY 2 cut(s) 124, 135
MhlI GDGCHC 1 cut(s) 709
MluCI AATT 1 cut(s) 462
MmeI TCCRAC 1 cut(s) 752
MnlI CCTC 9 cut(s) 47, 113, 226, 481, 514, 525, 592, 627, 696
MseI TTAA 2 cut(s) 96, 651
MslI CAYNNNNRTG 2 cut(s) 38, 629
MspI CCGG 8 cut(s) 23, 53, 133, 169, 210, 275, 342, 768
MspR9I CCNGG 5 cut(s) 133, 170, 275, 620, 674
MvaI CCWGG 2 cut(s) 620, 674
MvnI CGCG 4 cut(s) 48, 332, 356, 484
MwoI GCNNNNNNNGC 3 cut(s) 338, 389, 479
NciI CCSGG 3 cut(s) 133, 170, 275
NdeII GATC 6 cut(s) 60, 117, 124, 135, 217, 736
NlaIII CATG 5 cut(s) 413, 554, 628, 683, 713
NlaIV GGNNCC 1 cut(s) 391
NmeAIII GCCGAG 1 cut(s) 256
NmuCI GTSAC 3 cut(s) 70, 334, 365
NspV TTCGAA 2 cut(s) 348, 779
PagI TCATGA 1 cut(s) 709
PcsI WCGNNNNNNNCGW 3 cut(s) 45, 500, 506
PfeI GAWTC 2 cut(s) 161, 345
PflFI GACNNNGTC 2 cut(s) 275, 371
PflMI CCANNNNNTGG 2 cut(s) 556, 625
PkrI GCNGC 5 cut(s) 93, 229, 296, 316, 382
PshBI ATTAAT 1 cut(s) 651
Psp6I CCWGG 2 cut(s) 618, 672
PspEI GGTNACC 1 cut(s) 334
PspGI CCWGG 2 cut(s) 618, 672
PspN4I GGNNCC 1 cut(s) 391
PspPI GGNCC 5 cut(s) 4, 166, 198, 670, 676
PsuI RGATCY 2 cut(s) 124, 135
PsyI GACNNNGTC 2 cut(s) 275, 371
RsaI GTAC 2 cut(s) 190, 638
RsaNI GTAC 2 cut(s) 189, 637
RseI CAYNNNNRTG 2 cut(s) 38, 629
Rsr2I CGGWCCG 1 cut(s) 166
RsrII CGGWCCG 1 cut(s) 166
SaqAI TTAA 2 cut(s) 96, 651
SatI GCNGC 5 cut(s) 92, 228, 295, 315, 381
Sau3AI GATC 6 cut(s) 60, 117, 124, 135, 217, 736
Sau96I GGNCC 5 cut(s) 4, 166, 198, 670, 676
ScrFI CCNGG 5 cut(s) 133, 170, 275, 620, 674
SduI GDGCHC 1 cut(s) 709
SfaNI GCATC 1 cut(s) 741
SfuI TTCGAA 2 cut(s) 348, 779
SinI GGWCC 3 cut(s) 4, 166, 670
SmiMI CAYNNNNRTG 2 cut(s) 38, 629
Sse9I AATT 1 cut(s) 462
SsiI CCGC 9 cut(s) 46, 225, 228, 315, 332, 339, 381, 484, 518
SspI AATATT 1 cut(s) 716
StyD4I CCNGG 5 cut(s) 131, 168, 273, 618, 672
StyI CCWWGG 2 cut(s) 108, 732
TaaI ACNGT 1 cut(s) 83
TaiI ACGT 3 cut(s) 72, 240, 255
TaqI TCGA 6 cut(s) 216, 264, 348, 402, 458, 779
TaqII GACCGA 3 cut(s) 21, 154, 284
TasI AATT 1 cut(s) 462
TauI GCSGC 3 cut(s) 230, 317, 383
TfiI GAWTC 2 cut(s) 161, 345
Tru1I TTAA 2 cut(s) 96, 651
Tru9I TTAA 2 cut(s) 96, 651
TseFI GTSAC 3 cut(s) 70, 334, 365
TseI GCWGC 2 cut(s) 91, 294
Tsp45I GTSAC 3 cut(s) 70, 334, 365
TspDTI ATGAA 2 cut(s) 388, 726
TspGWI ACGGA 2 cut(s) 137, 420
Tth111I GACNNNGTC 2 cut(s) 275, 371
Van91I CCANNNNNTGG 2 cut(s) 556, 625
VpaK11BI GGWCC 3 cut(s) 4, 166, 670
VspI ATTAAT 1 cut(s) 651
XagI CCTNNNNNAGG 1 cut(s) 107
ZraI GACGTC 1 cut(s) 253
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.