RLG00000007365

enoyl-CoA hydratase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0012967
Physical Location & Seq
Reverse (-)
16145007 .. 16146656
1650 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000007365

Sequence Viewer

Length: 795 bp
ATGGACGGGAAACAATCTTCGCCGGAAAATCTCATTCTCGTCAACCGCGAACCGGACGGAATCGCTTTCGTCAGGATCAACCGTCCCAAGTCGCTTAACTTGCTGACCAAGGCTATGATCATGGATCTCGCCCGGGCCATCAAGTCCCTGAATCAAGACGATTCGGTCCGGGCCATTATTCTGTCGGGTACGGGTCGGGCCTTCTGCTCGGGCGTCAATCTCACCGCGGCCGAGGACGTTTTCAAAGGCGACGTCAAGGACATCAAGACCGCGGGGGCCCAAATAGAGCTATGCAAAAAGCCCATCATCACCGCCATCCACGGCTTCGCGGTAGCCGGCGGATTTCGAAATCGCGATCGGTTGCGACATCCTAAAGGAGCGAAGTTCATCGATACTCATGCCACATTTGGAGTACTTCCGTCGCTGGGTTTGTCTCAGAAGCTTTCGAGGATAATAGGGGCCAACAAAGCGCGTGAGATGTCACTGACGGCGACGCCGCTAACGGCGGAGGTGGTTGAGAGGTTGGGGTTAGTGAACCGTGTTGTGGAGGAGAGTGAGTTGCTGAAGAAAGCGCGGGAAATAGCAGAGGCGATTGTGAAGAATAATAACCAGGACTTGGTGTTGAGGTACAAGGCTGTTATTAATGATGGGCTTAAGCTGGACCTGGGACATGCTCTTACACTAGAAAGGGAGAGGGCTCATGAATATTACAATGGAATGACCAAGGATCAGTTTAAGAAGATGCAAGAGTTTATAGCCGGTCGTAGTACAGAAAAGCCTTCTTCCAAGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ECH_1

Protein Analysis

265

Amino Acids

29.07

Weight (kDa)

9.67

Isoelectric Point (pI)

30.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ECH_1 PF00378 18 - 239 5.3e-37 Enoyl-CoA hydratase/isomerase
ECH_2 PF16113 22 - 204 3.8e-18 Enoyl-CoA hydratase/isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012967)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G16210
fragaria_vesca FvH4_4g19880
malus_domestica MD16G1152800.v1.1
prunus_persica Prupe.1G116000_v2.0.a1
pyrus_communis pycom16g12990
rosa_chinensis RchiOBHm_Chr4g0425501 RchiOBHm_Chr4g0425511
rosa_laevigata RLG00000007359 RLG00000007363 RLG00000007365 RLG00000007367
rosa_multiflora Rmu_sc0001900.1_g000011
rosa_roxburghii Rroxscaffold_5G00367450
rosa_rugosa Rorug04G0203600
rosa_samantha Rh4AG260600 Rh4BG266300 Rh4DG262600
rosa_wichuraiana Rw4G022560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 164
AatII GACGTC 1 cut(s) 255
AccB7I CCANNNNNTGG 1 cut(s) 616
AccII CGCG 7 cut(s) 48, 227, 272, 329, 354, 472, 574
AclWI GGATC 3 cut(s) 83, 132, 735
AcoI YGGCCR 1 cut(s) 228
AcuI CTGAAG 1 cut(s) 584
AcyI GRCGYC 3 cut(s) 213, 252, 494
AfaI GTAC 4 cut(s) 190, 414, 629, 769
AfiI CCNNNNNNNGG 4 cut(s) 52, 425, 544, 616
AflII CTTAAG 1 cut(s) 653
AgsI TTSAA 1 cut(s) 244
AjnI CCWGG 2 cut(s) 609, 663
AluBI AGCT 3 cut(s) 289, 442, 658
AluI AGCT 3 cut(s) 289, 442, 658
Alw26I GTCTC 1 cut(s) 438
AlwI GGATC 3 cut(s) 83, 132, 735
Ama87I CYCGRG 2 cut(s) 132, 208
AoxI GGCC 6 cut(s) 135, 171, 198, 228, 276, 459
ApaI GGGCCC 1 cut(s) 280
ArsI GACNNNNNNTTYG 2 cut(s) 237, 269
AseI ATTAAT 1 cut(s) 642
AspLEI GCGC 2 cut(s) 472, 574
AspS9I GGNCC 8 cut(s) 135, 166, 171, 198, 276, 277, 459, 661
AsuC2I CCSGG 3 cut(s) 133, 134, 170
AsuHPI GGTGA 2 cut(s) 214, 301
AsuII TTCGAA 1 cut(s) 346
AvaI CYCGRG 2 cut(s) 132, 208
AvaII GGWCC 2 cut(s) 166, 661
BaeGI GKGCMC 1 cut(s) 280
BanII GRGCYC 2 cut(s) 280, 700
BccI CCATC 4 cut(s) 146, 311, 323, 641
BceAI ACGGC 3 cut(s) 337, 504, 519
BcgI CGANNNNNNTGC 2 cut(s) 380, 414
BciT130I CCWGG 2 cut(s) 611, 665
BclI TGATCA 1 cut(s) 117
BcnI CCSGG 3 cut(s) 133, 134, 170
BcoDI GTCTC 1 cut(s) 438
BfaI CTAG 1 cut(s) 683
BfrI CTTAAG 1 cut(s) 653
BisI GCNGC 2 cut(s) 228, 497
BlsI GCNGC 2 cut(s) 229, 498
BmcAI AGTACT 1 cut(s) 414
Bme1390I CCNGG 5 cut(s) 133, 134, 170, 611, 665
Bme18I GGWCC 2 cut(s) 166, 661
BmeT110I CYCGRG 2 cut(s) 132, 208
BmgT120I GGNCC 8 cut(s) 135, 166, 171, 198, 276, 277, 459, 661
BmiI GGNNCC 3 cut(s) 277, 278, 460
BmrFI CCNGG 5 cut(s) 133, 134, 170, 611, 665
BmsI GCATC 1 cut(s) 732
Bpu14I TTCGAA 1 cut(s) 346
BpuMI CCSGG 3 cut(s) 133, 134, 170
Bsa29I ATCGAT 1 cut(s) 390
BsaHI GRCGYC 3 cut(s) 213, 252, 494
BsaJI CCNNGG 8 cut(s) 108, 132, 225, 231, 270, 319, 664, 723
BsaWI WCCGGW 1 cut(s) 52
BsaXI ACNNNNNCTCC 2 cut(s) 542, 572
Bsc4I CCNNNNNNNGG 4 cut(s) 52, 425, 544, 616
Bse118I RCCGGY 2 cut(s) 335, 758
BseBI CCWGG 2 cut(s) 611, 665
BseCI ATCGAT 1 cut(s) 390
BseDI CCNNGG 8 cut(s) 108, 132, 225, 231, 270, 319, 664, 723
BseGI GGATG 2 cut(s) 315, 367
BseLI CCNNNNNNNGG 4 cut(s) 52, 425, 544, 616
BseMII CTCAG 1 cut(s) 449
BseRI GAGGAG 1 cut(s) 563
BseSI GKGCMC 1 cut(s) 280
BseX3I CGGCCG 1 cut(s) 228
BseYI CCCAGC 1 cut(s) 424
Bsh1236I CGCG 7 cut(s) 48, 227, 272, 329, 354, 472, 574
Bsh1285I CGRYCG 3 cut(s) 231, 358, 763
BshFI GGCC 6 cut(s) 137, 173, 200, 230, 278, 461
BshVI ATCGAT 1 cut(s) 390
BsiEI CGRYCG 3 cut(s) 231, 358, 763
BsiHKCI CYCGRG 2 cut(s) 132, 208
BsiSI CCGG 6 cut(s) 23, 53, 133, 169, 336, 759
BslFI GGGAC 3 cut(s) 69, 130, 681
BslI CCNNNNNNNGG 4 cut(s) 52, 425, 544, 616
BsmAI GTCTC 1 cut(s) 438
BsmFI GGGAC 3 cut(s) 69, 130, 681
BsnI GGCC 6 cut(s) 137, 173, 200, 230, 278, 461
BsoBI CYCGRG 2 cut(s) 132, 208
Bsp119I TTCGAA 1 cut(s) 346
Bsp120I GGGCCC 1 cut(s) 276
Bsp1286I GDGCHC 2 cut(s) 280, 700
Bsp143I GATC 5 cut(s) 75, 117, 124, 355, 727
Bsp68I TCGCGA 1 cut(s) 354
BspANI GGCC 6 cut(s) 137, 173, 200, 230, 278, 461
BspCNI CTCAG 1 cut(s) 448
BspDI ATCGAT 1 cut(s) 390
BspFNI CGCG 7 cut(s) 48, 227, 272, 329, 354, 472, 574
BspHI TCATGA 1 cut(s) 700
BspLI GGNNCC 3 cut(s) 277, 278, 460
BspPI GGATC 3 cut(s) 83, 132, 735
BspT104I TTCGAA 1 cut(s) 346
BspTI CTTAAG 1 cut(s) 653
BsrFI RCCGGY 2 cut(s) 335, 758
BssAI RCCGGY 2 cut(s) 335, 758
BssECI CCNNGG 8 cut(s) 108, 132, 225, 231, 270, 319, 664, 723
BssMI GATC 5 cut(s) 75, 117, 124, 355, 727
BssNI GRCGYC 3 cut(s) 213, 252, 494
BssT1I CCWWGG 2 cut(s) 108, 723
Bst2UI CCWGG 2 cut(s) 611, 665
Bst4CI ACNGT 2 cut(s) 83, 539
BstACI GRCGYC 3 cut(s) 213, 252, 494
BstAFI CTTAAG 1 cut(s) 653
BstBI TTCGAA 1 cut(s) 346
BstC8I GCNNGC 1 cut(s) 337
BstDEI CTNAG 1 cut(s) 435
BstDSI CCRYGG 3 cut(s) 225, 270, 319
BstF5I GGATG 2 cut(s) 315, 367
BstFNI CGCG 7 cut(s) 48, 227, 272, 329, 354, 472, 574
BstHHI GCGC 2 cut(s) 472, 574
BstKTI GATC 5 cut(s) 78, 120, 127, 358, 730
BstMAI GTCTC 1 cut(s) 438
BstMBI GATC 5 cut(s) 75, 117, 124, 355, 727
BstMCI CGRYCG 3 cut(s) 231, 358, 763
BstMWI GCNNNNNNNGC 2 cut(s) 100, 467
BstNI CCWGG 2 cut(s) 611, 665
BstNSI RCATGY 1 cut(s) 674
BstSCI CCNGG 5 cut(s) 131, 132, 168, 609, 663
BstSLI GKGCMC 1 cut(s) 280
BstUI CGCG 7 cut(s) 48, 227, 272, 329, 354, 472, 574
BstX2I RGATCY 1 cut(s) 124
BstYI RGATCY 1 cut(s) 124
BstZI CGGCCG 1 cut(s) 228
Bsu15I ATCGAT 1 cut(s) 390
BsuRI GGCC 6 cut(s) 137, 173, 200, 230, 278, 461
BsuTUI ATCGAT 1 cut(s) 390
BtgI CCRYGG 3 cut(s) 225, 270, 319
BtsCI GGATG 2 cut(s) 315, 367
BtsIMutI CAGTG 1 cut(s) 482
BtuMI TCGCGA 1 cut(s) 354
Cac8I GCNNGC 1 cut(s) 337
CciI TCATGA 1 cut(s) 700
CfoI GCGC 2 cut(s) 472, 574
Cfr10I RCCGGY 2 cut(s) 335, 758
Cfr13I GGNCC 8 cut(s) 135, 166, 171, 198, 276, 277, 459, 661
Cfr42I CCGCGG 2 cut(s) 228, 273
Cfr9I CCCGGG 1 cut(s) 132
ClaI ATCGAT 1 cut(s) 390
CpoI CGGWCCG 1 cut(s) 166
CseI GACGC 2 cut(s) 202, 502
Csp6I GTAC 4 cut(s) 189, 413, 628, 768
CspI CGGWCCG 1 cut(s) 166
CviAII CATG 4 cut(s) 121, 398, 671, 701
CviQI GTAC 4 cut(s) 189, 413, 628, 768
DdeI CTNAG 1 cut(s) 435
DpnI GATC 5 cut(s) 77, 119, 126, 357, 729
DpnII GATC 5 cut(s) 75, 117, 124, 355, 727
DrdI GACNNNNNNGTC 1 cut(s) 164
DseDI GACNNNNNNGTC 1 cut(s) 164
EaeI YGGCCR 1 cut(s) 228
EagI CGGCCG 1 cut(s) 228
EciI GGCGGA 2 cut(s) 354, 521
EclXI CGGCCG 1 cut(s) 228
Eco130I CCWWGG 2 cut(s) 108, 723
Eco24I GRGCYC 2 cut(s) 280, 700
Eco47I GGWCC 2 cut(s) 166, 661
Eco52I CGGCCG 1 cut(s) 228
Eco57I CTGAAG 1 cut(s) 584
Eco88I CYCGRG 2 cut(s) 132, 208
EcoO109I RGGNCCY 1 cut(s) 276
EcoRII CCWGG 2 cut(s) 609, 663
EcoT14I CCWWGG 2 cut(s) 108, 723
EcoT38I GRGCYC 2 cut(s) 280, 700
ErhI CCWWGG 2 cut(s) 108, 723
FaeI CATG 4 cut(s) 124, 401, 674, 704
FaiI YATR 7 cut(s) 116, 122, 292, 399, 672, 702, 755
FaqI GGGAC 3 cut(s) 69, 130, 681
FatI CATG 4 cut(s) 120, 397, 670, 700
FauI CCCGC 2 cut(s) 265, 567
FbaI TGATCA 1 cut(s) 117
Fnu4HI GCNGC 2 cut(s) 228, 497
FokI GGATG 2 cut(s) 302, 354
FriOI GRGCYC 2 cut(s) 280, 700
Fsp4HI GCNGC 2 cut(s) 228, 497
FspBI CTAG 1 cut(s) 683
GlaI GCGC 2 cut(s) 471, 573
GluI GCNGC 2 cut(s) 228, 497
GsaI CCCAGC 1 cut(s) 428
HaeIII GGCC 6 cut(s) 137, 173, 200, 230, 278, 461
HapII CCGG 6 cut(s) 23, 53, 133, 169, 336, 759
HgaI GACGC 2 cut(s) 202, 502
HhaI GCGC 2 cut(s) 472, 574
Hin1I GRCGYC 3 cut(s) 213, 252, 494
Hin1II CATG 4 cut(s) 124, 401, 674, 704
Hin6I GCGC 2 cut(s) 470, 572
HinP1I GCGC 2 cut(s) 470, 572
HincII GTYRAC 1 cut(s) 43
HindII GTYRAC 1 cut(s) 43
HindIII AAGCTT 1 cut(s) 440
HinfI GANTC 3 cut(s) 60, 151, 161
HpaII CCGG 6 cut(s) 23, 53, 133, 169, 336, 759
HphI GGTGA 2 cut(s) 214, 301
Hpy166II GTNNAC 2 cut(s) 43, 535
Hpy188I TCNGA 1 cut(s) 438
Hpy188III TCNNGA 5 cut(s) 73, 155, 265, 353, 701
Hpy8I GTNNAC 2 cut(s) 43, 535
Hpy99I CGWCG 3 cut(s) 254, 424, 496
HpyAV CCTTC 2 cut(s) 211, 789
HpyCH4III ACNGT 2 cut(s) 83, 539
HpyCH4IV ACGT 2 cut(s) 237, 252
HpyCH4V TGCA 2 cut(s) 294, 745
HpyF10VI GCNNNNNNNGC 2 cut(s) 100, 467
HpyF3I CTNAG 1 cut(s) 435
HpySE526I ACGT 2 cut(s) 237, 252
Hsp92I GRCGYC 3 cut(s) 213, 252, 494
Hsp92II CATG 4 cut(s) 124, 401, 674, 704
HspAI GCGC 2 cut(s) 470, 572
KroI GCCGGC 1 cut(s) 335
KroNI GCCGGC 1 cut(s) 337
Ksp22I TGATCA 1 cut(s) 117
KspI CCGCGG 2 cut(s) 228, 273
Kzo9I GATC 5 cut(s) 75, 117, 124, 355, 727
LmnI GCTCC 1 cut(s) 377
LweI GCATC 1 cut(s) 732
MaeI CTAG 1 cut(s) 683
MaeII ACGT 2 cut(s) 237, 252
MaeIII GTNAC 1 cut(s) 480
MalI GATC 5 cut(s) 77, 119, 126, 357, 729
MboI GATC 5 cut(s) 75, 117, 124, 355, 727
MboII GAAGA 5 cut(s) 9, 577, 610, 751, 774
MflI RGATCY 1 cut(s) 124
MhlI GDGCHC 2 cut(s) 280, 700
MnlI CCTC 8 cut(s) 226, 441, 502, 513, 541, 580, 618, 687
MroNI GCCGGC 1 cut(s) 335
MseI TTAA 4 cut(s) 96, 642, 654, 735
MspA1I CMGCKG 2 cut(s) 227, 272
MspCI CTTAAG 1 cut(s) 653
MspI CCGG 6 cut(s) 23, 53, 133, 169, 336, 759
MspR9I CCNGG 5 cut(s) 133, 134, 170, 611, 665
MvaI CCWGG 2 cut(s) 611, 665
MvnI CGCG 7 cut(s) 48, 227, 272, 329, 354, 472, 574
MwoI GCNNNNNNNGC 2 cut(s) 100, 467
NaeI GCCGGC 1 cut(s) 337
NciI CCSGG 3 cut(s) 133, 134, 170
NdeII GATC 5 cut(s) 75, 117, 124, 355, 727
NgoMIV GCCGGC 1 cut(s) 335
NlaIII CATG 4 cut(s) 124, 401, 674, 704
NlaIV GGNNCC 3 cut(s) 277, 278, 460
NmeAIII GCCGAG 1 cut(s) 256
NmuCI GTSAC 1 cut(s) 480
NruI TCGCGA 1 cut(s) 354
NspI RCATGY 1 cut(s) 674
NspV TTCGAA 1 cut(s) 346
PagI TCATGA 1 cut(s) 700
PcsI WCGNNNNNNNCGW 1 cut(s) 45
PdiI GCCGGC 1 cut(s) 337
PfeI GAWTC 3 cut(s) 60, 151, 161
PflMI CCANNNNNTGG 1 cut(s) 616
PkrI GCNGC 2 cut(s) 229, 498
Ple19I CGATCG 1 cut(s) 358
PshBI ATTAAT 1 cut(s) 642
Psp6I CCWGG 2 cut(s) 609, 663
PspFI CCCAGC 1 cut(s) 424
PspGI CCWGG 2 cut(s) 609, 663
PspN4I GGNNCC 3 cut(s) 277, 278, 460
PspOMI GGGCCC 1 cut(s) 276
PspPI GGNCC 8 cut(s) 135, 166, 171, 198, 276, 277, 459, 661
PsuI RGATCY 1 cut(s) 124
PvuI CGATCG 1 cut(s) 358
RruI TCGCGA 1 cut(s) 354
RsaI GTAC 4 cut(s) 190, 414, 629, 769
RsaNI GTAC 4 cut(s) 189, 413, 628, 768
Rsr2I CGGWCCG 1 cut(s) 166
RsrII CGGWCCG 1 cut(s) 166
SacII CCGCGG 2 cut(s) 228, 273
SaqAI TTAA 4 cut(s) 96, 642, 654, 735
SatI GCNGC 2 cut(s) 228, 497
Sau3AI GATC 5 cut(s) 75, 117, 124, 355, 727
Sau96I GGNCC 8 cut(s) 135, 166, 171, 198, 276, 277, 459, 661
ScaI AGTACT 1 cut(s) 414
ScrFI CCNGG 5 cut(s) 133, 134, 170, 611, 665
SduI GDGCHC 2 cut(s) 280, 700
SetI ASST 9 cut(s) 240, 255, 291, 444, 513, 524, 629, 660, 666
SfaNI GCATC 1 cut(s) 732
Sfr303I CCGCGG 2 cut(s) 228, 273
SfuI TTCGAA 1 cut(s) 346
SgrBI CCGCGG 2 cut(s) 228, 273
SinI GGWCC 2 cut(s) 166, 661
SmaI CCCGGG 1 cut(s) 134
SmlI CTYRAG 1 cut(s) 653
SmoI CTYRAG 1 cut(s) 653
SrfI GCCCGGGC 1 cut(s) 134
SspI AATATT 1 cut(s) 707
SspMI CTAG 1 cut(s) 683
StyD4I CCNGG 5 cut(s) 131, 132, 168, 609, 663
StyI CCWWGG 2 cut(s) 108, 723
TaaI ACNGT 2 cut(s) 83, 539
TaiI ACGT 2 cut(s) 240, 255
TaqI TCGA 3 cut(s) 346, 390, 446
TaqII GACCGA 1 cut(s) 154
TatI WGTACW 2 cut(s) 412, 767
TauI GCSGC 2 cut(s) 230, 499
TfiI GAWTC 3 cut(s) 60, 151, 161
Tru1I TTAA 4 cut(s) 96, 642, 654, 735
Tru9I TTAA 4 cut(s) 96, 642, 654, 735
TscAI CASTG 1 cut(s) 489
TseFI GTSAC 1 cut(s) 480
Tsp45I GTSAC 1 cut(s) 480
TspDTI ATGAA 2 cut(s) 376, 717
TspGWI ACGGA 2 cut(s) 72, 408
TspMI CCCGGG 1 cut(s) 132
TspRI CASTG 1 cut(s) 489
Van91I CCANNNNNTGG 1 cut(s) 616
Vha464I CTTAAG 1 cut(s) 653
VpaK11BI GGWCC 2 cut(s) 166, 661
VspI ATTAAT 1 cut(s) 642
XceI RCATGY 1 cut(s) 674
XmaI CCCGGG 1 cut(s) 132
XspI CTAG 1 cut(s) 683
ZraI GACGTC 1 cut(s) 253
ZrmI AGTACT 1 cut(s) 414
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.