Prupe.1G116000_v2.0.a1

enoyl-CoA hydratase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0012967
Physical Location & Seq
Reverse (-)
9153732 .. 9155816
2085 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G116000.1

Sequence Viewer

Length: 804 bp
ATGGCCAGCCACCAATCCTTGCCGGAAAATCTCATACTCGTAAACCGCGAACCCAACGGAATCGCATTCGTGACGATCAACCGTCCCAAGTCGCTTAATTCGCTGACCCGGCCCATGATTATAGACCTCGCCAAAGCCTTCAAGGCCCTCAGTCAAGACGAGTCGGTCCGGGTCATCATTCTATCCGGGTCGGGTCGCGCCTTTTGCTCCGGAGTCGATCTCACGGCCGCGGAGGACGTTTTCAAAGGGGACGTGAAGGACGTGGAGACCGACACGGTGGCGCAAATGGAGCGGTGCCGAAAGCCCATCATCGGAGCCATTAATGGGTTCGCGGTCACTGCCGGGTTCGAAATCGCGCTCGCTTGTGATATAATCGTCGCCGCAAAAGGAGCTAAATTCGTGGACACTCATGCCAGGTTTGGGATATTTCCGTCATGGGGTTTGTCTCAGAAGCTTTCGAGGATTATAGGGGCTAACAAAGCTCGGGAAGTGTCGCTGACAGCGACGCCTTTGACGGCGGAGCTGGGTGAAAGGTTGGGGTTCGTGAACCATGTTGTTGAAGAGGGTGAGTTGCTGAAGAAAGCTCGTGAAATAGCTGAGGCCATTGCGAAAAATAACCAGGACCTGGTGTTGAGGTACAAGGCTGTTATTAATGACGGGCTTAAACTGGACCTAGGCCATGCTCTTGCACTAGAAAAGGAGAGGGGTCATGACTATTACAGTGGAATGACCAAAGAGCAGTTTAAGAAGATGCAAGAGTTCATAACAGGTCGGGGTTCAAAAAAGCCTTCTTCCAAGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ECH_1

Protein Analysis

268

Amino Acids

29.09

Weight (kDa)

9.03

Isoelectric Point (pI)

20.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012967)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G16210
fragaria_vesca FvH4_4g19880
malus_domestica MD16G1152800.v1.1
prunus_persica Prupe.1G116000_v2.0.a1
pyrus_communis pycom16g12990
rosa_chinensis RchiOBHm_Chr4g0425501 RchiOBHm_Chr4g0425511
rosa_laevigata RLG00000007359 RLG00000007363 RLG00000007365 RLG00000007367
rosa_multiflora Rmu_sc0001900.1_g000011
rosa_roxburghii Rroxscaffold_5G00367450
rosa_rugosa Rorug04G0203600
rosa_samantha Rh4AG260600 Rh4BG266300 Rh4DG262600
rosa_wichuraiana Rw4G022560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 164
AccB1I GGYRCC 1 cut(s) 294
AccB7I CCANNNNNTGG 1 cut(s) 625
AccBSI CCGCTC 1 cut(s) 292
AccII CGCG 5 cut(s) 48, 198, 230, 332, 356
AccIII TCCGGA 1 cut(s) 209
AciI CCGC 7 cut(s) 46, 228, 230, 292, 332, 381, 518
AcoI YGGCCR 2 cut(s) 3, 225
AcsI RAATTY 1 cut(s) 395
AcuI CTGAAG 1 cut(s) 596
AcyI GRCGYC 1 cut(s) 506
AfaI GTAC 1 cut(s) 638
AfiI CCNNNNNNNGG 5 cut(s) 311, 324, 420, 437, 625
AgsI TTSAA 4 cut(s) 142, 244, 560, 780
AjiI CACGTC 2 cut(s) 253, 262
AjnI CCWGG 3 cut(s) 413, 618, 624
AluBI AGCT 6 cut(s) 392, 454, 482, 523, 584, 596
AluI AGCT 6 cut(s) 392, 454, 482, 523, 584, 596
Alw26I GTCTC 2 cut(s) 260, 450
AlwNI CAGNNNCTG 1 cut(s) 625
Ama87I CYCGRG 1 cut(s) 483
Aor13HI TCCGGA 1 cut(s) 209
AoxI GGCC 6 cut(s) 3, 110, 144, 225, 600, 676
ApoI RAATTY 1 cut(s) 395
AseI ATTAAT 2 cut(s) 321, 651
AspA2I CCTAGG 1 cut(s) 673
AspLEI GCGC 3 cut(s) 200, 283, 358
AspS9I GGNCC 5 cut(s) 111, 145, 166, 622, 670
AsuC2I CCSGG 4 cut(s) 109, 170, 187, 343
AsuHPI GGTGA 2 cut(s) 539, 578
AsuII TTCGAA 1 cut(s) 348
AvaI CYCGRG 1 cut(s) 483
AvaII GGWCC 3 cut(s) 166, 622, 670
AvrII CCTAGG 1 cut(s) 673
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 294
BauI CACGAG 1 cut(s) 585
BbvCI CCTCAGC 1 cut(s) 597
BccI CCATC 1 cut(s) 314
BceAI ACGGC 2 cut(s) 240, 531
BciT130I CCWGG 3 cut(s) 415, 620, 626
BcnI CCSGG 4 cut(s) 109, 170, 187, 343
BcoDI GTCTC 2 cut(s) 260, 450
BfaI CTAG 2 cut(s) 674, 692
BglI GCCNNNNNGGC 1 cut(s) 143
BisI GCNGC 2 cut(s) 228, 381
BlnI CCTAGG 1 cut(s) 673
BlsI GCNGC 2 cut(s) 229, 382
Bme1390I CCNGG 7 cut(s) 109, 170, 187, 343, 415, 620, 626
Bme18I GGWCC 3 cut(s) 166, 622, 670
BmeT110I CYCGRG 1 cut(s) 483
BmgBI CACGTC 2 cut(s) 253, 262
BmgT120I GGNCC 5 cut(s) 111, 145, 166, 622, 670
BmiI GGNNCC 2 cut(s) 296, 316
BmrFI CCNGG 7 cut(s) 109, 170, 187, 343, 415, 620, 626
BmsI GCATC 1 cut(s) 741
BplI GAGNNNNNCTC 2 cut(s) 204, 236
Bpu10I CCTNAGC 1 cut(s) 597
Bpu14I TTCGAA 1 cut(s) 348
BpuMI CCSGG 4 cut(s) 109, 170, 187, 343
BsaHI GRCGYC 1 cut(s) 506
BsaI GGTCTC 1 cut(s) 260
BsaJI CCNNGG 2 cut(s) 228, 673
BsaWI WCCGGW 1 cut(s) 209
Bsc4I CCNNNNNNNGG 5 cut(s) 311, 324, 420, 437, 625
Bse1I ACTGG 1 cut(s) 672
Bse3DI GCAATG 1 cut(s) 603
BseAI TCCGGA 1 cut(s) 209
BseBI CCWGG 3 cut(s) 415, 620, 626
BseDI CCNNGG 2 cut(s) 228, 673
BseLI CCNNNNNNNGG 5 cut(s) 311, 324, 420, 437, 625
BseMI GCAATG 1 cut(s) 603
BseMII CTCAG 3 cut(s) 163, 461, 588
BseNI ACTGG 1 cut(s) 672
BseX3I CGGCCG 1 cut(s) 225
BseYI CCCAGC 1 cut(s) 523
Bsh1236I CGCG 5 cut(s) 48, 198, 230, 332, 356
Bsh1285I CGRYCG 1 cut(s) 228
BshFI GGCC 6 cut(s) 5, 112, 146, 227, 602, 678
BshNI GGYRCC 1 cut(s) 294
BsiEI CGRYCG 1 cut(s) 228
BsiHKCI CYCGRG 1 cut(s) 483
BsiSI CCGG 6 cut(s) 23, 109, 169, 186, 210, 342
BslFI GGGAC 2 cut(s) 69, 263
BslI CCNNNNNNNGG 5 cut(s) 311, 324, 420, 437, 625
BsmAI GTCTC 2 cut(s) 260, 450
BsmFI GGGAC 2 cut(s) 69, 263
BsmI GAATGC 1 cut(s) 65
BsnI GGCC 6 cut(s) 5, 112, 146, 227, 602, 678
Bso31I GGTCTC 1 cut(s) 260
BsoBI CYCGRG 1 cut(s) 483
Bsp119I TTCGAA 1 cut(s) 348
Bsp13I TCCGGA 1 cut(s) 209
Bsp143I GATC 2 cut(s) 75, 217
BspACI CCGC 7 cut(s) 46, 228, 230, 292, 332, 381, 518
BspANI GGCC 6 cut(s) 5, 112, 146, 227, 602, 678
BspCNI CTCAG 3 cut(s) 162, 460, 589
BspEI TCCGGA 1 cut(s) 209
BspFNI CGCG 5 cut(s) 48, 198, 230, 332, 356
BspHI TCATGA 1 cut(s) 709
BspLI GGNNCC 2 cut(s) 296, 316
BspT104I TTCGAA 1 cut(s) 348
BspT107I GGYRCC 1 cut(s) 294
BspTNI GGTCTC 1 cut(s) 260
BsrBI CCGCTC 1 cut(s) 292
BsrDI GCAATG 1 cut(s) 603
BsrI ACTGG 1 cut(s) 672
BssECI CCNNGG 2 cut(s) 228, 673
BssMI GATC 2 cut(s) 75, 217
BssNI GRCGYC 1 cut(s) 506
BssSI CACGAG 1 cut(s) 585
BssT1I CCWWGG 1 cut(s) 673
Bst2BI CACGAG 1 cut(s) 585
Bst2UI CCWGG 3 cut(s) 415, 620, 626
Bst4CI ACNGT 3 cut(s) 83, 277, 722
Bst6I CTCTTC 1 cut(s) 555
BstACI GRCGYC 1 cut(s) 506
BstBI TTCGAA 1 cut(s) 348
BstC8I GCNNGC 2 cut(s) 7, 360
BstDEI CTNAG 3 cut(s) 149, 447, 597
BstDSI CCRYGG 1 cut(s) 228
BstFNI CGCG 5 cut(s) 48, 198, 230, 332, 356
BstHHI GCGC 3 cut(s) 200, 283, 358
BstKTI GATC 2 cut(s) 78, 220
BstMAI GTCTC 2 cut(s) 260, 450
BstMBI GATC 2 cut(s) 75, 217
BstMCI CGRYCG 1 cut(s) 228
BstMWI GCNNNNNNNGC 8 cut(s) 100, 109, 143, 204, 289, 338, 389, 479
BstNI CCWGG 3 cut(s) 415, 620, 626
BstSCI CCNGG 7 cut(s) 107, 168, 185, 341, 413, 618, 624
BstUI CGCG 5 cut(s) 48, 198, 230, 332, 356
BstZI CGGCCG 1 cut(s) 225
BsuRI GGCC 6 cut(s) 5, 112, 146, 227, 602, 678
BtgI CCRYGG 1 cut(s) 228
BtrI CACGTC 2 cut(s) 253, 262
BtsI GCAGTG 1 cut(s) 336
BtsIMutI CAGTG 2 cut(s) 336, 727
Cac8I GCNNGC 2 cut(s) 7, 360
CaiI CAGNNNCTG 1 cut(s) 625
CciI TCATGA 1 cut(s) 709
CfoI GCGC 3 cut(s) 200, 283, 358
Cfr13I GGNCC 5 cut(s) 111, 145, 166, 622, 670
Cfr42I CCGCGG 1 cut(s) 231
CpoI CGGWCCG 1 cut(s) 166
CseI GACGC 1 cut(s) 514
CsiI ACCWGGT 1 cut(s) 624
Csp6I GTAC 1 cut(s) 637
CspI CGGWCCG 1 cut(s) 166
CviAII CATG 6 cut(s) 115, 410, 435, 551, 680, 710
CviQI GTAC 1 cut(s) 637
DdeI CTNAG 3 cut(s) 149, 447, 597
DpnI GATC 2 cut(s) 77, 219
DpnII GATC 2 cut(s) 75, 217
DrdI GACNNNNNNGTC 1 cut(s) 164
DseDI GACNNNNNNGTC 1 cut(s) 164
EaeI YGGCCR 2 cut(s) 3, 225
EagI CGGCCG 1 cut(s) 225
Eam1104I CTCTTC 1 cut(s) 555
EarI CTCTTC 1 cut(s) 555
EciI GGCGGA 1 cut(s) 533
EclXI CGGCCG 1 cut(s) 225
Eco130I CCWWGG 1 cut(s) 673
Eco31I GGTCTC 1 cut(s) 260
Eco47I GGWCC 3 cut(s) 166, 622, 670
Eco52I CGGCCG 1 cut(s) 225
Eco57I CTGAAG 1 cut(s) 596
Eco88I CYCGRG 1 cut(s) 483
EcoO109I RGGNCCY 2 cut(s) 145, 622
EcoRII CCWGG 3 cut(s) 413, 618, 624
EcoT14I CCWWGG 1 cut(s) 673
ErhI CCWWGG 1 cut(s) 673
FaeI CATG 6 cut(s) 118, 413, 438, 554, 683, 713
FaqI GGGAC 2 cut(s) 69, 263
FatI CATG 6 cut(s) 114, 409, 434, 550, 679, 709
Fnu4HI GCNGC 2 cut(s) 228, 381
Fsp4HI GCNGC 2 cut(s) 228, 381
FspBI CTAG 2 cut(s) 674, 692
GlaI GCGC 3 cut(s) 199, 282, 357
GluI GCNGC 2 cut(s) 228, 381
GsaI CCCAGC 1 cut(s) 527
HaeIII GGCC 6 cut(s) 5, 112, 146, 227, 602, 678
HapII CCGG 6 cut(s) 23, 109, 169, 186, 210, 342
HgaI GACGC 1 cut(s) 514
HhaI GCGC 3 cut(s) 200, 283, 358
Hin1I GRCGYC 1 cut(s) 506
Hin1II CATG 6 cut(s) 118, 413, 438, 554, 683, 713
Hin6I GCGC 3 cut(s) 198, 281, 356
HinP1I GCGC 3 cut(s) 198, 281, 356
HindIII AAGCTT 1 cut(s) 452
HinfI GANTC 3 cut(s) 60, 161, 213
HpaII CCGG 6 cut(s) 23, 109, 169, 186, 210, 342
HphI GGTGA 2 cut(s) 539, 578
Hpy166II GTNNAC 3 cut(s) 43, 403, 547
Hpy188I TCNGA 2 cut(s) 314, 450
Hpy188III TCNNGA 7 cut(s) 70, 155, 210, 485, 544, 587, 710
Hpy8I GTNNAC 3 cut(s) 43, 403, 547
Hpy99I CGWCG 2 cut(s) 380, 508
HpyAV CCTTC 3 cut(s) 148, 250, 798
HpyCH4III ACNGT 3 cut(s) 83, 277, 722
HpyCH4IV ACGT 3 cut(s) 237, 252, 261
HpyCH4V TGCA 2 cut(s) 689, 754
HpyF10VI GCNNNNNNNGC 8 cut(s) 100, 109, 143, 204, 289, 338, 389, 479
HpyF3I CTNAG 3 cut(s) 149, 447, 597
HpySE526I ACGT 3 cut(s) 237, 252, 261
Hsp92I GRCGYC 1 cut(s) 506
Hsp92II CATG 6 cut(s) 118, 413, 438, 554, 683, 713
HspAI GCGC 3 cut(s) 198, 281, 356
Kpn2I TCCGGA 1 cut(s) 209
KspI CCGCGG 1 cut(s) 231
Kzo9I GATC 2 cut(s) 75, 217
LmnI GCTCC 5 cut(s) 212, 289, 314, 389, 520
LweI GCATC 1 cut(s) 741
MabI ACCWGGT 1 cut(s) 624
MaeI CTAG 2 cut(s) 674, 692
MaeII ACGT 3 cut(s) 237, 252, 261
MaeIII GTNAC 2 cut(s) 70, 334
MalI GATC 2 cut(s) 77, 219
MbiI CCGCTC 1 cut(s) 292
MboI GATC 2 cut(s) 75, 217
MboII GAAGA 4 cut(s) 572, 589, 760, 783
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 97, 395
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 170, 222
MnlI CCTC 8 cut(s) 137, 158, 226, 453, 556, 592, 627, 696
Mox20I TGGCCA 1 cut(s) 5
MroI TCCGGA 1 cut(s) 209
MscI TGGCCA 1 cut(s) 5
MseI TTAA 5 cut(s) 96, 321, 651, 663, 744
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 230
MspI CCGG 6 cut(s) 23, 109, 169, 186, 210, 342
MspR9I CCNGG 7 cut(s) 109, 170, 187, 343, 415, 620, 626
Mva1269I GAATGC 1 cut(s) 65
MvaI CCWGG 3 cut(s) 415, 620, 626
MvnI CGCG 5 cut(s) 48, 198, 230, 332, 356
MwoI GCNNNNNNNGC 8 cut(s) 100, 109, 143, 204, 289, 338, 389, 479
NciI CCSGG 4 cut(s) 109, 170, 187, 343
NdeII GATC 2 cut(s) 75, 217
NlaIII CATG 6 cut(s) 118, 413, 438, 554, 683, 713
NlaIV GGNNCC 2 cut(s) 296, 316
NmuCI GTSAC 2 cut(s) 70, 334
NspV TTCGAA 1 cut(s) 348
PagI TCATGA 1 cut(s) 709
PcsI WCGNNNNNNNCGW 4 cut(s) 45, 258, 267, 500
PctI GAATGC 1 cut(s) 65
PfeI GAWTC 1 cut(s) 60
PflMI CCANNNNNTGG 1 cut(s) 625
PkrI GCNGC 2 cut(s) 229, 382
PleI GAGTC 2 cut(s) 169, 221
PpsI GAGTC 2 cut(s) 169, 221
PpuMI RGGWCCY 1 cut(s) 622
PshBI ATTAAT 2 cut(s) 321, 651
Psp5II RGGWCCY 1 cut(s) 622
Psp6I CCWGG 3 cut(s) 413, 618, 624
PspFI CCCAGC 1 cut(s) 523
PspGI CCWGG 3 cut(s) 413, 618, 624
PspN4I GGNNCC 2 cut(s) 296, 316
PspPI GGNCC 5 cut(s) 111, 145, 166, 622, 670
PspPPI RGGWCCY 1 cut(s) 622
PstNI CAGNNNCTG 1 cut(s) 625
RsaI GTAC 1 cut(s) 638
RsaNI GTAC 1 cut(s) 637
Rsr2I CGGWCCG 1 cut(s) 166
RsrII CGGWCCG 1 cut(s) 166
SacII CCGCGG 1 cut(s) 231
SaqAI TTAA 5 cut(s) 96, 321, 651, 663, 744
SatI GCNGC 2 cut(s) 228, 381
Sau3AI GATC 2 cut(s) 75, 217
Sau96I GGNCC 5 cut(s) 111, 145, 166, 622, 670
SchI GAGTC 2 cut(s) 170, 222
ScrFI CCNGG 7 cut(s) 109, 170, 187, 343, 415, 620, 626
SexAI ACCWGGT 1 cut(s) 624
SfaNI GCATC 1 cut(s) 741
Sfr303I CCGCGG 1 cut(s) 231
SfuI TTCGAA 1 cut(s) 348
SgrBI CCGCGG 1 cut(s) 231
SinI GGWCC 3 cut(s) 166, 622, 670
Sse9I AATT 2 cut(s) 97, 395
SsiI CCGC 7 cut(s) 46, 228, 230, 292, 332, 381, 518
SspMI CTAG 2 cut(s) 674, 692
StyD4I CCNGG 7 cut(s) 107, 168, 185, 341, 413, 618, 624
StyI CCWWGG 1 cut(s) 673
TaaI ACNGT 3 cut(s) 83, 277, 722
TaiI ACGT 3 cut(s) 240, 255, 264
TaqI TCGA 3 cut(s) 216, 348, 458
TaqII GACCGA 2 cut(s) 154, 284
TasI AATT 2 cut(s) 97, 395
TauI GCSGC 2 cut(s) 230, 383
TfiI GAWTC 1 cut(s) 60
Tru1I TTAA 5 cut(s) 96, 321, 651, 663, 744
Tru9I TTAA 5 cut(s) 96, 321, 651, 663, 744
TscAI CASTG 2 cut(s) 343, 727
TseFI GTSAC 2 cut(s) 70, 334
Tsp45I GTSAC 2 cut(s) 70, 334
TspDTI ATGAA 1 cut(s) 751
TspGWI ACGGA 2 cut(s) 72, 420
TspRI CASTG 2 cut(s) 343, 727
Van91I CCANNNNNTGG 1 cut(s) 625
VpaK11BI GGWCC 3 cut(s) 166, 622, 670
VspI ATTAAT 2 cut(s) 321, 651
XapI RAATTY 1 cut(s) 395
XmaJI CCTAGG 1 cut(s) 673
XspI CTAG 2 cut(s) 674, 692
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.