Rroxscaffold_5G00367450

enoyl-CoA hydratase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0012967
Physical Location & Seq
Forward (+)
49296887 .. 49298745
1859 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00367450.1

Sequence Viewer

Length: 753 bp
ATGGACGGGAAACAGTCTTCGCCGGAAAATCTCATTCTCGTCAACCGCGAACCGGACGGAGTCGCCTTCGTCACCATCAACCGTCCCAAGTTGCTCAACTCGCTGACCAAGGCTATGATCACGGATCTCGCCGGGGCCATCAAGTCCCTGAATCAAGACGATTCGGTCCGGGTCATTGTTCTGTCGGGTACGGGTCGGGCCTTCTGCTCCGGCGTCGATCTCACCGCGGCCGAGGACGTTTTCAAAGGCGACGTCAAGGACATCGAGGCCGACCCGGTGGCCCAAATGGAGCGGTGCAAAAAGCCTATCATCGCCGCCATCAACGGCTTCGCGGTCACCGCCGGGATTCGAAATCGCGCTCGCCTATTTGGGATATTTCCGTCGTGGGGTTTGTCTCAGAAGCTTTCGAGGATAATAGGGGCAAACAAAGCGCGTGAGGTGTCGCTGACGGCGACGCCGCTAACGGCGGAGGTGGGTGAGAGGTTGGGGTTTGTGAACCATGTTGTGGAGGAGAGTGAGTTGCTGAAGAAAGCGCGGGAAATAGCTGGGGCCATTGTGAAGAATAACCAGGACCTGGTGTTGAGGTACAAGGCTGTTATTAATGATGGGCTCAAGCTGGACCTGGGTCATGCTCTTACACTAGAACAGGAGAGGGCTCATGAATATTACAATGGAATGACCAAGGATCAGTTTAAGAAGATGCAAGAGTTTATAGCCGGTCGGAGTTCAAAAAAGCCTTCTTCCAAGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ECH_1

Protein Analysis

250

Amino Acids

27.29

Weight (kDa)

9.26

Isoelectric Point (pI)

26.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ECH_1 PF00378 18 - 116 3e-22 Enoyl-CoA hydratase/isomerase
ECH_2 PF16113 22 - 115 9.3e-17 Enoyl-CoA hydratase/isomerase
ECH_1 PF00378 122 - 225 7.8e-13 Enoyl-CoA hydratase/isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012967)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G16210
fragaria_vesca FvH4_4g19880
malus_domestica MD16G1152800.v1.1
prunus_persica Prupe.1G116000_v2.0.a1
pyrus_communis pycom16g12990
rosa_chinensis RchiOBHm_Chr4g0425501 RchiOBHm_Chr4g0425511
rosa_laevigata RLG00000007359 RLG00000007363 RLG00000007365 RLG00000007367
rosa_multiflora Rmu_sc0001900.1_g000011
rosa_roxburghii Rroxscaffold_5G00367450
rosa_rugosa Rorug04G0203600
rosa_samantha Rh4AG260600 Rh4BG266300 Rh4DG262600
rosa_wichuraiana Rw4G022560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 164
AatII GACGTC 1 cut(s) 255
AccB7I CCANNNNNTGG 2 cut(s) 505, 574
AccBSI CCGCTC 1 cut(s) 292
AccII CGCG 6 cut(s) 48, 227, 332, 357, 433, 535
AclWI GGATC 2 cut(s) 132, 693
AcoI YGGCCR 1 cut(s) 228
AcuI CTGAAG 1 cut(s) 545
AcyI GRCGYC 3 cut(s) 213, 252, 455
AfaI GTAC 2 cut(s) 190, 587
AfiI CCNNNNNNNGG 4 cut(s) 52, 386, 505, 574
AgsI TTSAA 2 cut(s) 244, 729
AjnI CCWGG 3 cut(s) 567, 573, 621
AluBI AGCT 3 cut(s) 403, 545, 616
AluI AGCT 3 cut(s) 403, 545, 616
Alw26I GTCTC 1 cut(s) 399
AlwI GGATC 2 cut(s) 132, 693
AlwNI CAGNNNCTG 1 cut(s) 574
AoxI GGCC 6 cut(s) 135, 198, 228, 267, 279, 549
ArsI GACNNNNNNTTYG 2 cut(s) 237, 269
AseI ATTAAT 1 cut(s) 600
AspLEI GCGC 3 cut(s) 359, 433, 535
AspS9I GGNCC 7 cut(s) 135, 166, 198, 280, 549, 571, 619
AsuC2I CCSGG 4 cut(s) 133, 170, 275, 343
AsuHPI GGTGA 4 cut(s) 64, 214, 328, 488
AsuII TTCGAA 1 cut(s) 349
AvaII GGWCC 3 cut(s) 166, 571, 619
BanII GRGCYC 2 cut(s) 612, 658
BbsI GAAGAC 1 cut(s) 9
BccI CCATC 4 cut(s) 83, 146, 326, 599
BceAI ACGGC 3 cut(s) 340, 465, 480
BciT130I CCWGG 3 cut(s) 569, 575, 623
BclI TGATCA 1 cut(s) 117
BcnI CCSGG 4 cut(s) 133, 170, 275, 343
BcoDI GTCTC 1 cut(s) 399
BfaI CTAG 1 cut(s) 641
BisI GCNGC 3 cut(s) 228, 315, 458
BlsI GCNGC 3 cut(s) 229, 316, 459
Bme1390I CCNGG 7 cut(s) 133, 170, 275, 343, 569, 575, 623
Bme18I GGWCC 3 cut(s) 166, 571, 619
BmgT120I GGNCC 7 cut(s) 135, 166, 198, 280, 549, 571, 619
BmiI GGNNCC 2 cut(s) 136, 550
BmrFI CCNGG 7 cut(s) 133, 170, 275, 343, 569, 575, 623
BmsI GCATC 1 cut(s) 690
BoxI GACNNNNGTC 1 cut(s) 624
BpiI GAAGAC 1 cut(s) 9
Bpu14I TTCGAA 1 cut(s) 349
BpuEI CTTGAG 1 cut(s) 596
BpuMI CCSGG 4 cut(s) 133, 170, 275, 343
BsaHI GRCGYC 3 cut(s) 213, 252, 455
BsaJI CCNNGG 6 cut(s) 108, 132, 225, 231, 622, 681
BsaWI WCCGGW 1 cut(s) 52
BsaXI ACNNNNNCTCC 2 cut(s) 503, 533
Bsc4I CCNNNNNNNGG 4 cut(s) 52, 386, 505, 574
Bse118I RCCGGY 1 cut(s) 716
BseBI CCWGG 3 cut(s) 569, 575, 623
BseDI CCNNGG 6 cut(s) 108, 132, 225, 231, 622, 681
BseLI CCNNNNNNNGG 4 cut(s) 52, 386, 505, 574
BseMII CTCAG 1 cut(s) 410
BseRI GAGGAG 1 cut(s) 524
BseX3I CGGCCG 1 cut(s) 228
BseYI CCCAGC 1 cut(s) 545
Bsh1236I CGCG 6 cut(s) 48, 227, 332, 357, 433, 535
Bsh1285I CGRYCG 2 cut(s) 231, 721
BshFI GGCC 6 cut(s) 137, 200, 230, 269, 281, 551
BsiEI CGRYCG 2 cut(s) 231, 721
BsiSI CCGG 8 cut(s) 23, 53, 132, 169, 210, 275, 342, 717
BslFI GGGAC 2 cut(s) 69, 130
BslI CCNNNNNNNGG 4 cut(s) 52, 386, 505, 574
BsmAI GTCTC 1 cut(s) 399
BsmFI GGGAC 2 cut(s) 69, 130
BsnI GGCC 6 cut(s) 137, 200, 230, 269, 281, 551
Bsp119I TTCGAA 1 cut(s) 349
Bsp1286I GDGCHC 2 cut(s) 612, 658
Bsp143I GATC 4 cut(s) 117, 124, 217, 685
BspANI GGCC 6 cut(s) 137, 200, 230, 269, 281, 551
BspCNI CTCAG 1 cut(s) 409
BspFNI CGCG 6 cut(s) 48, 227, 332, 357, 433, 535
BspHI TCATGA 1 cut(s) 658
BspLI GGNNCC 2 cut(s) 136, 550
BspPI GGATC 2 cut(s) 132, 693
BspT104I TTCGAA 1 cut(s) 349
BsrBI CCGCTC 1 cut(s) 292
BsrFI RCCGGY 1 cut(s) 716
BssAI RCCGGY 1 cut(s) 716
BssECI CCNNGG 6 cut(s) 108, 132, 225, 231, 622, 681
BssMI GATC 4 cut(s) 117, 124, 217, 685
BssNI GRCGYC 3 cut(s) 213, 252, 455
BssT1I CCWWGG 2 cut(s) 108, 681
Bst2UI CCWGG 3 cut(s) 569, 575, 623
Bst4CI ACNGT 2 cut(s) 15, 83
BstACI GRCGYC 3 cut(s) 213, 252, 455
BstBI TTCGAA 1 cut(s) 349
BstC8I GCNNGC 1 cut(s) 361
BstDEI CTNAG 1 cut(s) 396
BstDSI CCRYGG 1 cut(s) 225
BstEII GGTNACC 1 cut(s) 334
BstFNI CGCG 6 cut(s) 48, 227, 332, 357, 433, 535
BstHHI GCGC 3 cut(s) 359, 433, 535
BstKTI GATC 4 cut(s) 120, 127, 220, 688
BstMAI GTCTC 1 cut(s) 399
BstMBI GATC 4 cut(s) 117, 124, 217, 685
BstMCI CGRYCG 2 cut(s) 231, 721
BstMWI GCNNNNNNNGC 3 cut(s) 100, 338, 428
BstNI CCWGG 3 cut(s) 569, 575, 623
BstPAI GACNNNNGTC 1 cut(s) 624
BstPI GGTNACC 1 cut(s) 334
BstSCI CCNGG 7 cut(s) 131, 168, 273, 341, 567, 573, 621
BstUI CGCG 6 cut(s) 48, 227, 332, 357, 433, 535
BstV2I GAAGAC 1 cut(s) 9
BstX2I RGATCY 1 cut(s) 124
BstYI RGATCY 1 cut(s) 124
BstZI CGGCCG 1 cut(s) 228
BsuRI GGCC 6 cut(s) 137, 200, 230, 269, 281, 551
BtgI CCRYGG 1 cut(s) 225
BtgZI GCGATG 1 cut(s) 295
Cac8I GCNNGC 1 cut(s) 361
CaiI CAGNNNCTG 1 cut(s) 574
CciI TCATGA 1 cut(s) 658
CfoI GCGC 3 cut(s) 359, 433, 535
Cfr10I RCCGGY 1 cut(s) 716
Cfr13I GGNCC 7 cut(s) 135, 166, 198, 280, 549, 571, 619
Cfr42I CCGCGG 1 cut(s) 228
CpoI CGGWCCG 1 cut(s) 166
CseI GACGC 2 cut(s) 202, 463
CsiI ACCWGGT 1 cut(s) 573
Csp6I GTAC 2 cut(s) 189, 586
CspI CGGWCCG 1 cut(s) 166
CviAII CATG 3 cut(s) 500, 629, 659
CviQI GTAC 2 cut(s) 189, 586
DdeI CTNAG 1 cut(s) 396
DpnI GATC 4 cut(s) 119, 126, 219, 687
DpnII GATC 4 cut(s) 117, 124, 217, 685
DrdI GACNNNNNNGTC 1 cut(s) 164
DseDI GACNNNNNNGTC 1 cut(s) 164
EaeI YGGCCR 1 cut(s) 228
EagI CGGCCG 1 cut(s) 228
EciI GGCGGA 1 cut(s) 482
EclXI CGGCCG 1 cut(s) 228
Eco130I CCWWGG 2 cut(s) 108, 681
Eco24I GRGCYC 2 cut(s) 612, 658
Eco47I GGWCC 3 cut(s) 166, 571, 619
Eco52I CGGCCG 1 cut(s) 228
Eco57I CTGAAG 1 cut(s) 545
Eco91I GGTNACC 1 cut(s) 334
EcoO109I RGGNCCY 1 cut(s) 571
EcoO65I GGTNACC 1 cut(s) 334
EcoRII CCWGG 3 cut(s) 567, 573, 621
EcoT14I CCWWGG 2 cut(s) 108, 681
EcoT38I GRGCYC 2 cut(s) 612, 658
ErhI CCWWGG 2 cut(s) 108, 681
FaeI CATG 3 cut(s) 503, 632, 662
FaiI YATR 5 cut(s) 116, 501, 630, 660, 713
FaqI GGGAC 2 cut(s) 69, 130
FatI CATG 3 cut(s) 499, 628, 658
FauI CCCGC 1 cut(s) 528
FbaI TGATCA 1 cut(s) 117
Fnu4HI GCNGC 3 cut(s) 228, 315, 458
FriOI GRGCYC 2 cut(s) 612, 658
Fsp4HI GCNGC 3 cut(s) 228, 315, 458
FspBI CTAG 1 cut(s) 641
GlaI GCGC 3 cut(s) 358, 432, 534
GluI GCNGC 3 cut(s) 228, 315, 458
GsaI CCCAGC 1 cut(s) 549
HaeIII GGCC 6 cut(s) 137, 200, 230, 269, 281, 551
HapII CCGG 8 cut(s) 23, 53, 132, 169, 210, 275, 342, 717
HgaI GACGC 2 cut(s) 202, 463
HhaI GCGC 3 cut(s) 359, 433, 535
Hin1I GRCGYC 3 cut(s) 213, 252, 455
Hin1II CATG 3 cut(s) 503, 632, 662
Hin6I GCGC 3 cut(s) 357, 431, 533
HinP1I GCGC 3 cut(s) 357, 431, 533
HincII GTYRAC 1 cut(s) 43
HindII GTYRAC 1 cut(s) 43
HindIII AAGCTT 1 cut(s) 401
HinfI GANTC 4 cut(s) 60, 151, 161, 346
HpaII CCGG 8 cut(s) 23, 53, 132, 169, 210, 275, 342, 717
HphI GGTGA 4 cut(s) 64, 214, 328, 488
Hpy166II GTNNAC 2 cut(s) 43, 496
Hpy188I TCNGA 2 cut(s) 399, 723
Hpy188III TCNNGA 2 cut(s) 155, 659
Hpy8I GTNNAC 2 cut(s) 43, 496
Hpy99I CGWCG 4 cut(s) 218, 254, 385, 457
HpyAV CCTTC 3 cut(s) 76, 211, 747
HpyCH4III ACNGT 2 cut(s) 15, 83
HpyCH4IV ACGT 2 cut(s) 237, 252
HpyCH4V TGCA 2 cut(s) 297, 703
HpyF10VI GCNNNNNNNGC 3 cut(s) 100, 338, 428
HpyF3I CTNAG 1 cut(s) 396
HpySE526I ACGT 2 cut(s) 237, 252
Hsp92I GRCGYC 3 cut(s) 213, 252, 455
Hsp92II CATG 3 cut(s) 503, 632, 662
HspAI GCGC 3 cut(s) 357, 431, 533
Ksp22I TGATCA 1 cut(s) 117
KspI CCGCGG 1 cut(s) 228
Kzo9I GATC 4 cut(s) 117, 124, 217, 685
LmnI GCTCC 2 cut(s) 212, 289
LweI GCATC 1 cut(s) 690
MabI ACCWGGT 1 cut(s) 573
MaeI CTAG 1 cut(s) 641
MaeII ACGT 2 cut(s) 237, 252
MaeIII GTNAC 2 cut(s) 70, 334
MalI GATC 4 cut(s) 119, 126, 219, 687
MbiI CCGCTC 1 cut(s) 292
MboI GATC 4 cut(s) 117, 124, 217, 685
MboII GAAGA 5 cut(s) 9, 538, 571, 709, 732
MflI RGATCY 1 cut(s) 124
MhlI GDGCHC 2 cut(s) 612, 658
MlyI GAGTC 1 cut(s) 69
MmeI TCCRAC 1 cut(s) 701
MnlI CCTC 9 cut(s) 226, 259, 402, 430, 463, 474, 502, 576, 645
MseI TTAA 2 cut(s) 600, 693
MspA1I CMGCKG 1 cut(s) 227
MspI CCGG 8 cut(s) 23, 53, 132, 169, 210, 275, 342, 717
MspR9I CCNGG 7 cut(s) 133, 170, 275, 343, 569, 575, 623
MvaI CCWGG 3 cut(s) 569, 575, 623
MvnI CGCG 6 cut(s) 48, 227, 332, 357, 433, 535
MwoI GCNNNNNNNGC 3 cut(s) 100, 338, 428
NciI CCSGG 4 cut(s) 133, 170, 275, 343
NdeII GATC 4 cut(s) 117, 124, 217, 685
NlaIII CATG 3 cut(s) 503, 632, 662
NlaIV GGNNCC 2 cut(s) 136, 550
NmeAIII GCCGAG 1 cut(s) 256
NmuCI GTSAC 2 cut(s) 70, 334
NspV TTCGAA 1 cut(s) 349
PagI TCATGA 1 cut(s) 658
PcsI WCGNNNNNNNCGW 2 cut(s) 45, 449
PfeI GAWTC 3 cut(s) 151, 161, 346
PflFI GACNNNGTC 1 cut(s) 59
PflMI CCANNNNNTGG 2 cut(s) 505, 574
PkrI GCNGC 3 cut(s) 229, 316, 459
PleI GAGTC 1 cut(s) 68
PpsI GAGTC 1 cut(s) 68
PpuMI RGGWCCY 1 cut(s) 571
PshAI GACNNNNGTC 1 cut(s) 624
PshBI ATTAAT 1 cut(s) 600
Psp5II RGGWCCY 1 cut(s) 571
Psp6I CCWGG 3 cut(s) 567, 573, 621
PspEI GGTNACC 1 cut(s) 334
PspFI CCCAGC 1 cut(s) 545
PspGI CCWGG 3 cut(s) 567, 573, 621
PspN4I GGNNCC 2 cut(s) 136, 550
PspPI GGNCC 7 cut(s) 135, 166, 198, 280, 549, 571, 619
PspPPI RGGWCCY 1 cut(s) 571
PstNI CAGNNNCTG 1 cut(s) 574
PsuI RGATCY 1 cut(s) 124
PsyI GACNNNGTC 1 cut(s) 59
RsaI GTAC 2 cut(s) 190, 587
RsaNI GTAC 2 cut(s) 189, 586
Rsr2I CGGWCCG 1 cut(s) 166
RsrII CGGWCCG 1 cut(s) 166
SacII CCGCGG 1 cut(s) 228
SaqAI TTAA 2 cut(s) 600, 693
SatI GCNGC 3 cut(s) 228, 315, 458
Sau3AI GATC 4 cut(s) 117, 124, 217, 685
Sau96I GGNCC 7 cut(s) 135, 166, 198, 280, 549, 571, 619
SchI GAGTC 1 cut(s) 69
ScrFI CCNGG 7 cut(s) 133, 170, 275, 343, 569, 575, 623
SduI GDGCHC 2 cut(s) 612, 658
SexAI ACCWGGT 1 cut(s) 573
SfaNI GCATC 1 cut(s) 690
Sfr303I CCGCGG 1 cut(s) 228
SfuI TTCGAA 1 cut(s) 349
SgrBI CCGCGG 1 cut(s) 228
SinI GGWCC 3 cut(s) 166, 571, 619
SmlI CTYRAG 1 cut(s) 611
SmoI CTYRAG 1 cut(s) 611
SspI AATATT 1 cut(s) 665
SspMI CTAG 1 cut(s) 641
StyD4I CCNGG 7 cut(s) 131, 168, 273, 341, 567, 573, 621
StyI CCWWGG 2 cut(s) 108, 681
TaaI ACNGT 2 cut(s) 15, 83
TaiI ACGT 2 cut(s) 240, 255
TaqI TCGA 4 cut(s) 216, 264, 349, 407
TaqII GACCGA 1 cut(s) 154
TauI GCSGC 3 cut(s) 230, 317, 460
TfiI GAWTC 3 cut(s) 151, 161, 346
Tru1I TTAA 2 cut(s) 600, 693
Tru9I TTAA 2 cut(s) 600, 693
TseFI GTSAC 2 cut(s) 70, 334
Tsp45I GTSAC 2 cut(s) 70, 334
TspDTI ATGAA 1 cut(s) 675
TspGWI ACGGA 3 cut(s) 72, 137, 369
Tth111I GACNNNGTC 1 cut(s) 59
Van91I CCANNNNNTGG 2 cut(s) 505, 574
VpaK11BI GGWCC 3 cut(s) 166, 571, 619
VspI ATTAAT 1 cut(s) 600
XspI CTAG 1 cut(s) 641
ZraI GACGTC 1 cut(s) 253
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.