Rorug04G0203600

enoyl-CoA hydratase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0012967
Physical Location & Seq
Reverse (-)
35396085 .. 35401292
5208 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0203600.1

Sequence Viewer

Length: 618 bp
ATGCTTTGCAGAGGAGTTGAATCCATGATTCACAAGGAAATCTTTATGGGAAGGTTTAGGAATCATCAACTCATATATAAAGCAAGGTCCCTGTTCTCACAATTAATCAAGCAATATTGTCTACCCCATTATAGACTGCTAAAGCATTGGAGAGAACAGAAGACTTGTGCTTTCGGCATTGCGATGGCTTCTACATCATCAGAATGGAGTGTAATCAATGACACTAGTTCGTCGTTTGCATTTCAAGGAAATAAGAAATGCGATTTTGACACTTCTTTGGCATTTAATCGGTCAGTGCTTTCTTGCCGCTGCCCACAAGGGTTTGATGGAAATCCCTATCTTCTCCAACCTTGTCAAGACATTGATGAGTGCATAGATGGTACTAAACGATGCTGGCCCGGCAGCAAGTGCATGAACCTTCCTGGTTCGTTCAAATGCGAGGACGAAAAGGATACAATTAAATTGGCACTTGCAGGTCTTGGCTCGAGTCTTGGACTGTTATTATTACTTATTGCTGCATGGTGGGTACACAAACTGGTGAAGAAAAGGAAAAGCATTAAACGCAAGGAAATGTCATTCAAAAGTGTAGATCCTAGAAGGAGCCTAGAGGGGGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ECH_1

Protein Analysis

205

Amino Acids

23.38

Weight (kDa)

9.36

Isoelectric Point (pI)

48.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EGF_CA PF07645 120 - 147 5.2e-07 Calcium-binding EGF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012967)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G16210
fragaria_vesca FvH4_4g19880
malus_domestica MD16G1152800.v1.1
prunus_persica Prupe.1G116000_v2.0.a1
pyrus_communis pycom16g12990
rosa_chinensis RchiOBHm_Chr4g0425501 RchiOBHm_Chr4g0425511
rosa_laevigata RLG00000007359 RLG00000007363 RLG00000007365 RLG00000007367
rosa_multiflora Rmu_sc0001900.1_g000011
rosa_roxburghii Rroxscaffold_5G00367450
rosa_rugosa Rorug04G0203600
rosa_samantha Rh4AG260600 Rh4BG266300 Rh4DG262600
rosa_wichuraiana Rw4G022560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 464
AccI GTMKAC 1 cut(s) 121
AciI CCGC 1 cut(s) 307
AclWI GGATC 1 cut(s) 584
AfaI GTAC 2 cut(s) 382, 528
AfiI CCNNNNNNNGG 1 cut(s) 610
AgsI TTSAA 4 cut(s) 20, 245, 433, 580
AhlI ACTAGT 1 cut(s) 224
AjnI CCWGG 1 cut(s) 421
AlwI GGATC 1 cut(s) 584
Ama87I CYCGRG 1 cut(s) 484
AoxI GGCC 1 cut(s) 395
ApeKI GCWGC 3 cut(s) 309, 402, 515
AseI ATTAAT 1 cut(s) 104
AspS9I GGNCC 2 cut(s) 87, 396
AsuC2I CCSGG 1 cut(s) 399
AsuHPI GGTGA 1 cut(s) 550
AvaI CYCGRG 1 cut(s) 484
AvaII GGWCC 1 cut(s) 87
BbsI GAAGAC 1 cut(s) 167
BbvI GCAGC 3 cut(s) 296, 414, 502
BccI CCATC 3 cut(s) 178, 320, 371
BciT130I CCWGG 1 cut(s) 423
BciVI GTATCC 1 cut(s) 445
BcnI CCSGG 1 cut(s) 399
BcuI ACTAGT 1 cut(s) 224
BfaI CTAG 3 cut(s) 225, 594, 605
BfuAI ACCTGC 1 cut(s) 464
BfuI GTATCC 1 cut(s) 445
BisI GCNGC 4 cut(s) 307, 310, 403, 516
BlsI GCNGC 4 cut(s) 308, 311, 404, 517
Bme1390I CCNGG 2 cut(s) 399, 423
Bme18I GGWCC 1 cut(s) 87
BmeT110I CYCGRG 1 cut(s) 484
BmgT120I GGNCC 2 cut(s) 87, 396
BmiI GGNNCC 2 cut(s) 89, 602
BmrFI CCNGG 2 cut(s) 399, 423
BmsI GCATC 1 cut(s) 380
BpiI GAAGAC 1 cut(s) 167
BpuMI CCSGG 1 cut(s) 399
BsaBI GATNNNNATC 1 cut(s) 330
Bsc4I CCNNNNNNNGG 1 cut(s) 610
Bse1I ACTGG 1 cut(s) 540
Bse3DI GCAATG 1 cut(s) 177
Bse8I GATNNNNATC 1 cut(s) 330
BseBI CCWGG 1 cut(s) 423
BseJI GATNNNNATC 1 cut(s) 330
BseLI CCNNNNNNNGG 1 cut(s) 610
BseMI GCAATG 1 cut(s) 177
BseNI ACTGG 1 cut(s) 540
BseRI GAGGAG 1 cut(s) 27
BseXI GCAGC 3 cut(s) 296, 414, 502
BshFI GGCC 1 cut(s) 397
BsiHKCI CYCGRG 1 cut(s) 484
BsiSI CCGG 1 cut(s) 399
BslFI GGGAC 1 cut(s) 73
BslI CCNNNNNNNGG 1 cut(s) 610
BsmFI GGGAC 1 cut(s) 73
BsnI GGCC 1 cut(s) 397
BsoBI CYCGRG 1 cut(s) 484
Bsp143I GATC 1 cut(s) 589
BspACI CCGC 1 cut(s) 307
BspANI GGCC 1 cut(s) 397
BspLI GGNNCC 2 cut(s) 89, 602
BspMI ACCTGC 1 cut(s) 464
BspPI GGATC 1 cut(s) 584
BsrDI GCAATG 1 cut(s) 177
BsrI ACTGG 1 cut(s) 540
BssMI GATC 1 cut(s) 589
Bst2UI CCWGG 1 cut(s) 423
Bst4CI ACNGT 1 cut(s) 498
BstAPI GCANNNNNTGC 1 cut(s) 408
BstC8I GCNNGC 1 cut(s) 395
BstKTI GATC 1 cut(s) 592
BstMBI GATC 1 cut(s) 589
BstMWI GCNNNNNNNGC 3 cut(s) 399, 408, 561
BstNI CCWGG 1 cut(s) 423
BstSCI CCNGG 2 cut(s) 397, 421
BstV1I GCAGC 3 cut(s) 296, 414, 502
BstV2I GAAGAC 1 cut(s) 167
BstX2I RGATCY 1 cut(s) 589
BstYI RGATCY 1 cut(s) 589
BsuI GTATCC 1 cut(s) 445
BsuRI GGCC 1 cut(s) 397
BtgZI GCGATG 1 cut(s) 197
BtsIMutI CAGTG 1 cut(s) 300
BveI ACCTGC 1 cut(s) 464
Cac8I GCNNGC 1 cut(s) 395
Cfr13I GGNCC 2 cut(s) 87, 396
Csp6I GTAC 2 cut(s) 381, 527
CviAII CATG 3 cut(s) 25, 412, 519
CviJI RGCY 4 cut(s) 188, 397, 483, 603
CviKI_1 RGCY 4 cut(s) 188, 397, 483, 603
CviQI GTAC 2 cut(s) 381, 527
DpnI GATC 1 cut(s) 591
DpnII GATC 1 cut(s) 589
Eco47I GGWCC 1 cut(s) 87
Eco88I CYCGRG 1 cut(s) 484
EcoO109I RGGNCCY 1 cut(s) 87
EcoRII CCWGG 1 cut(s) 421
FaeI CATG 3 cut(s) 28, 415, 522
FaiI YATR 9 cut(s) 26, 47, 74, 76, 78, 132, 374, 413, 520
FalI AAGNNNNNCTT 2 cut(s) 26, 58
FaqI GGGAC 1 cut(s) 73
FatI CATG 3 cut(s) 24, 411, 518
FblI GTMKAC 1 cut(s) 121
Fnu4HI GCNGC 4 cut(s) 307, 310, 403, 516
Fsp4HI GCNGC 4 cut(s) 307, 310, 403, 516
FspBI CTAG 3 cut(s) 225, 594, 605
GluI GCNGC 4 cut(s) 307, 310, 403, 516
HaeIII GGCC 1 cut(s) 397
HapII CCGG 1 cut(s) 399
Hin1II CATG 3 cut(s) 28, 415, 522
HinfI GANTC 4 cut(s) 20, 28, 61, 487
HpaII CCGG 1 cut(s) 399
HphI GGTGA 1 cut(s) 550
Hpy166II GTNNAC 2 cut(s) 122, 529
Hpy188I TCNGA 1 cut(s) 202
Hpy188III TCNNGA 1 cut(s) 356
Hpy8I GTNNAC 2 cut(s) 122, 529
Hpy99I CGWCG 1 cut(s) 235
HpyAV CCTTC 3 cut(s) 45, 428, 591
HpyCH4III ACNGT 1 cut(s) 498
HpyCH4V TGCA 6 cut(s) 9, 239, 372, 411, 473, 518
HpyF10VI GCNNNNNNNGC 3 cut(s) 399, 408, 561
Hsp92II CATG 3 cut(s) 28, 415, 522
Kzo9I GATC 1 cut(s) 589
LmnI GCTCC 1 cut(s) 600
LpnPI CCDG 7 cut(s) 104, 379, 408, 412, 435, 459, 521
Lsp1109I GCAGC 3 cut(s) 296, 414, 502
LweI GCATC 1 cut(s) 380
MaeI CTAG 3 cut(s) 225, 594, 605
MalI GATC 1 cut(s) 591
MboI GATC 1 cut(s) 589
MboII GAAGA 3 cut(s) 172, 332, 553
MflI RGATCY 1 cut(s) 589
MluCI AATT 3 cut(s) 101, 456, 461
MlyI GAGTC 1 cut(s) 496
MmeI TCCRAC 1 cut(s) 370
MnlI CCTC 3 cut(s) 5, 433, 601
MseI TTAA 4 cut(s) 104, 285, 459, 558
MslI CAYNNNNRTG 2 cut(s) 182, 202
MspA1I CMGCKG 1 cut(s) 309
MspI CCGG 1 cut(s) 399
MspR9I CCNGG 2 cut(s) 399, 423
MvaI CCWGG 1 cut(s) 423
MwoI GCNNNNNNNGC 3 cut(s) 399, 408, 561
NciI CCSGG 1 cut(s) 399
NdeII GATC 1 cut(s) 589
NlaIII CATG 3 cut(s) 28, 415, 522
NlaIV GGNNCC 2 cut(s) 89, 602
PaeR7I CTCGAG 1 cut(s) 484
PfeI GAWTC 3 cut(s) 20, 28, 61
PkrI GCNGC 4 cut(s) 308, 311, 404, 517
PleI GAGTC 1 cut(s) 495
PpsI GAGTC 1 cut(s) 495
PpuMI RGGWCCY 1 cut(s) 87
PshBI ATTAAT 1 cut(s) 104
Psp5II RGGWCCY 1 cut(s) 87
Psp6I CCWGG 1 cut(s) 421
PspGI CCWGG 1 cut(s) 421
PspN4I GGNNCC 2 cut(s) 89, 602
PspPI GGNCC 2 cut(s) 87, 396
PspPPI RGGWCCY 1 cut(s) 87
PspXI VCTCGAGB 1 cut(s) 484
PsuI RGATCY 1 cut(s) 589
RsaI GTAC 2 cut(s) 382, 528
RsaNI GTAC 2 cut(s) 381, 527
RseI CAYNNNNRTG 2 cut(s) 182, 202
SaqAI TTAA 4 cut(s) 104, 285, 459, 558
SatI GCNGC 4 cut(s) 307, 310, 403, 516
Sau3AI GATC 1 cut(s) 589
Sau96I GGNCC 2 cut(s) 87, 396
SchI GAGTC 1 cut(s) 496
ScrFI CCNGG 2 cut(s) 399, 423
SetI ASST 5 cut(s) 56, 89, 352, 420, 478
SfaNI GCATC 1 cut(s) 380
Sfr274I CTCGAG 1 cut(s) 484
SinI GGWCC 1 cut(s) 87
SlaI CTCGAG 1 cut(s) 484
SmiMI CAYNNNNRTG 2 cut(s) 182, 202
SmlI CTYRAG 1 cut(s) 484
SmoI CTYRAG 1 cut(s) 484
SpeI ACTAGT 1 cut(s) 224
Sse9I AATT 3 cut(s) 101, 456, 461
SsiI CCGC 1 cut(s) 307
SspI AATATT 1 cut(s) 116
SspMI CTAG 3 cut(s) 225, 594, 605
StyD4I CCNGG 2 cut(s) 397, 421
TaaI ACNGT 1 cut(s) 498
TaqI TCGA 1 cut(s) 485
TaqII GACCGA 1 cut(s) 279
TasI AATT 3 cut(s) 101, 456, 461
TauI GCSGC 1 cut(s) 309
TfiI GAWTC 3 cut(s) 20, 28, 61
Tru1I TTAA 4 cut(s) 104, 285, 459, 558
Tru9I TTAA 4 cut(s) 104, 285, 459, 558
TscAI CASTG 1 cut(s) 300
TseI GCWGC 3 cut(s) 309, 402, 515
TspDTI ATGAA 1 cut(s) 428
TspRI CASTG 1 cut(s) 300
VpaK11BI GGWCC 1 cut(s) 87
VspI ATTAAT 1 cut(s) 104
XhoI CTCGAG 1 cut(s) 484
XmiI GTMKAC 1 cut(s) 121
XspI CTAG 3 cut(s) 225, 594, 605
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.