Rh4BG266300

enoyl-CoA hydratase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0012967
Physical Location & Seq
Forward (+)
44048931 .. 44050797
1867 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG266300.1

Sequence Viewer

Length: 804 bp
ATGGACGGGAAACAATCTTCGCCGGAAAATCTCATTCTCGTCAACCGCGAACCAGACGGAGTCGCCTTCGTCACGATCAACCGTCCCAAGTTGCTCAACTCGCTGACCAAGGCTATGATCACGGATCTCGCCCGGGCCATCAAGTCCCTCAATCAAGACGATTCGGTCCGGGTCATTGTTCTGTCGGGTGCCGGTAGGGCCTTCTGCTCCGGCGTCGATCTCACCGCTGCCGAGGACGTTTTCAAAGGCGACGTCAAGGACATCGAGGCCGACCCGGTGGCCCAAATGGAGCGGTGCAAAAAGCCCATCATCGCCGCCATCAACGGCTTCGCGGTCACCGCCGGATTCGAAATCGCGCTCGCCTGTGACATCATCGTCGCCGCTAAAGGAGCGAAGTTCATCGACACTCACGCCAGATTTGGGATATTTCCGTCGTGGGGTTTGTCTCAGAAGCTTTCGAGGATAATAGGGGCCAACAAAGCGCGTGAGGTGTCGCTGACGGCGACGCCGCTAACGGCGGAGGTGGGTGAGAGGTTGGGGTTTGTGAACCATGTTGTGGAGGAGAGTGAGTTGCTGAAGAAAGCGCGGGAAGTAGCTGGGGCCATTGTGAAGAATAACCAGGACCTGGTGTTGAGGTACAAGGCTGTTATTAATGATGGGCTTAAGGTGGACCTGGGTCATGCTCTTACACTAGAAAAGGAGAGGGCTCATGAATATTACAATGGAATGACCAAGGATCAGTTTAAGAAGATGCAAGAGTTTATAGCCGGTCGGAGTTCAAAAAAGCCTTCTTCCAAGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ECH_1

Protein Analysis

267

Amino Acids

29.01

Weight (kDa)

9.01

Isoelectric Point (pI)

24.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ECH_1 PF00378 18 - 242 7.4e-52 Enoyl-CoA hydratase/isomerase
ECH_2 PF16113 22 - 199 3.2e-26 Enoyl-CoA hydratase/isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012967)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G16210
fragaria_vesca FvH4_4g19880
malus_domestica MD16G1152800.v1.1
prunus_persica Prupe.1G116000_v2.0.a1
pyrus_communis pycom16g12990
rosa_chinensis RchiOBHm_Chr4g0425501 RchiOBHm_Chr4g0425511
rosa_laevigata RLG00000007359 RLG00000007363 RLG00000007365 RLG00000007367
rosa_multiflora Rmu_sc0001900.1_g000011
rosa_roxburghii Rroxscaffold_5G00367450
rosa_rugosa Rorug04G0203600
rosa_samantha Rh4AG260600 Rh4BG266300 Rh4DG262600
rosa_wichuraiana Rw4G022560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 164, 374
AatII GACGTC 1 cut(s) 255
AccB1I GGYRCC 1 cut(s) 188
AccB7I CCANNNNNTGG 2 cut(s) 556, 625
AccBSI CCGCTC 1 cut(s) 292
AccII CGCG 5 cut(s) 48, 332, 356, 484, 586
AclWI GGATC 2 cut(s) 132, 744
AcuI CTGAAG 1 cut(s) 596
AcyI GRCGYC 3 cut(s) 213, 252, 506
AfaI GTAC 1 cut(s) 638
AfiI CCNNNNNNNGG 4 cut(s) 420, 437, 556, 625
AflII CTTAAG 1 cut(s) 662
AgsI TTSAA 2 cut(s) 244, 780
AjnI CCWGG 3 cut(s) 618, 624, 672
AluBI AGCT 2 cut(s) 454, 596
AluI AGCT 2 cut(s) 454, 596
Alw26I GTCTC 1 cut(s) 450
AlwI GGATC 2 cut(s) 132, 744
AlwNI CAGNNNCTG 1 cut(s) 625
Ama87I CYCGRG 1 cut(s) 132
AoxI GGCC 6 cut(s) 135, 198, 267, 279, 471, 600
ApeKI GCWGC 1 cut(s) 227
ArsI GACNNNNNNTTYG 2 cut(s) 237, 269
AseI ATTAAT 1 cut(s) 651
AspLEI GCGC 3 cut(s) 358, 484, 586
AspS9I GGNCC 8 cut(s) 135, 166, 198, 280, 471, 600, 622, 670
AsuC2I CCSGG 4 cut(s) 133, 134, 170, 275
AsuHPI GGTGA 3 cut(s) 214, 328, 539
AsuII TTCGAA 1 cut(s) 348
AvaI CYCGRG 1 cut(s) 132
AvaII GGWCC 3 cut(s) 166, 622, 670
BanI GGYRCC 1 cut(s) 188
BanII GRGCYC 1 cut(s) 709
BbvI GCAGC 1 cut(s) 214
BccI CCATC 4 cut(s) 146, 314, 326, 650
BceAI ACGGC 3 cut(s) 340, 516, 531
BciT130I CCWGG 3 cut(s) 620, 626, 674
BclI TGATCA 1 cut(s) 117
BcnI CCSGG 4 cut(s) 133, 134, 170, 275
BcoDI GTCTC 1 cut(s) 450
BfaI CTAG 1 cut(s) 692
BfrI CTTAAG 1 cut(s) 662
BglI GCCNNNNNGGC 1 cut(s) 197
BisI GCNGC 4 cut(s) 228, 315, 381, 509
BlsI GCNGC 4 cut(s) 229, 316, 382, 510
Bme1390I CCNGG 7 cut(s) 133, 134, 170, 275, 620, 626, 674
Bme18I GGWCC 3 cut(s) 166, 622, 670
BmeT110I CYCGRG 1 cut(s) 132
BmgT120I GGNCC 8 cut(s) 135, 166, 198, 280, 471, 600, 622, 670
BmiI GGNNCC 3 cut(s) 190, 472, 601
BmrFI CCNGG 7 cut(s) 133, 134, 170, 275, 620, 626, 674
BmsI GCATC 1 cut(s) 741
BoxI GACNNNNGTC 1 cut(s) 675
Bpu14I TTCGAA 1 cut(s) 348
BpuMI CCSGG 4 cut(s) 133, 134, 170, 275
BsaHI GRCGYC 3 cut(s) 213, 252, 506
BsaJI CCNNGG 5 cut(s) 108, 132, 231, 673, 732
BsaXI ACNNNNNCTCC 2 cut(s) 554, 584
Bsc4I CCNNNNNNNGG 4 cut(s) 420, 437, 556, 625
Bse118I RCCGGY 2 cut(s) 191, 767
BseBI CCWGG 3 cut(s) 620, 626, 674
BseDI CCNNGG 5 cut(s) 108, 132, 231, 673, 732
BseLI CCNNNNNNNGG 4 cut(s) 420, 437, 556, 625
BseMII CTCAG 1 cut(s) 461
BseRI GAGGAG 1 cut(s) 575
BseXI GCAGC 1 cut(s) 214
BseYI CCCAGC 1 cut(s) 596
Bsh1236I CGCG 5 cut(s) 48, 332, 356, 484, 586
Bsh1285I CGRYCG 1 cut(s) 772
BshFI GGCC 6 cut(s) 137, 200, 269, 281, 473, 602
BshNI GGYRCC 1 cut(s) 188
BsiEI CGRYCG 1 cut(s) 772
BsiHKCI CYCGRG 1 cut(s) 132
BsiSI CCGG 8 cut(s) 23, 133, 169, 192, 210, 275, 342, 768
BslFI GGGAC 2 cut(s) 69, 130
BslI CCNNNNNNNGG 4 cut(s) 420, 437, 556, 625
BsmAI GTCTC 1 cut(s) 450
BsmFI GGGAC 2 cut(s) 69, 130
BsnI GGCC 6 cut(s) 137, 200, 269, 281, 473, 602
BsoBI CYCGRG 1 cut(s) 132
Bsp119I TTCGAA 1 cut(s) 348
Bsp1286I GDGCHC 1 cut(s) 709
Bsp143I GATC 5 cut(s) 75, 117, 124, 217, 736
BspANI GGCC 6 cut(s) 137, 200, 269, 281, 473, 602
BspCNI CTCAG 1 cut(s) 460
BspFNI CGCG 5 cut(s) 48, 332, 356, 484, 586
BspHI TCATGA 1 cut(s) 709
BspLI GGNNCC 3 cut(s) 190, 472, 601
BspPI GGATC 2 cut(s) 132, 744
BspT104I TTCGAA 1 cut(s) 348
BspT107I GGYRCC 1 cut(s) 188
BspTI CTTAAG 1 cut(s) 662
BsrBI CCGCTC 1 cut(s) 292
BsrFI RCCGGY 2 cut(s) 191, 767
BssAI RCCGGY 2 cut(s) 191, 767
BssECI CCNNGG 5 cut(s) 108, 132, 231, 673, 732
BssMI GATC 5 cut(s) 75, 117, 124, 217, 736
BssNI GRCGYC 3 cut(s) 213, 252, 506
BssT1I CCWWGG 2 cut(s) 108, 732
Bst2UI CCWGG 3 cut(s) 620, 626, 674
Bst4CI ACNGT 1 cut(s) 83
BstACI GRCGYC 3 cut(s) 213, 252, 506
BstAFI CTTAAG 1 cut(s) 662
BstBI TTCGAA 1 cut(s) 348
BstC8I GCNNGC 1 cut(s) 360
BstDEI CTNAG 1 cut(s) 447
BstEII GGTNACC 1 cut(s) 334
BstFNI CGCG 5 cut(s) 48, 332, 356, 484, 586
BstHHI GCGC 3 cut(s) 358, 484, 586
BstKTI GATC 5 cut(s) 78, 120, 127, 220, 739
BstMAI GTCTC 1 cut(s) 450
BstMBI GATC 5 cut(s) 75, 117, 124, 217, 736
BstMCI CGRYCG 1 cut(s) 772
BstMWI GCNNNNNNNGC 5 cut(s) 100, 197, 338, 389, 479
BstNI CCWGG 3 cut(s) 620, 626, 674
BstPAI GACNNNNGTC 1 cut(s) 675
BstPI GGTNACC 1 cut(s) 334
BstSCI CCNGG 7 cut(s) 131, 132, 168, 273, 618, 624, 672
BstUI CGCG 5 cut(s) 48, 332, 356, 484, 586
BstV1I GCAGC 1 cut(s) 214
BstX2I RGATCY 1 cut(s) 124
BstYI RGATCY 1 cut(s) 124
BsuRI GGCC 6 cut(s) 137, 200, 269, 281, 473, 602
BtgZI GCGATG 1 cut(s) 295
Cac8I GCNNGC 1 cut(s) 360
CaiI CAGNNNCTG 1 cut(s) 625
CciI TCATGA 1 cut(s) 709
CfoI GCGC 3 cut(s) 358, 484, 586
Cfr10I RCCGGY 2 cut(s) 191, 767
Cfr13I GGNCC 8 cut(s) 135, 166, 198, 280, 471, 600, 622, 670
Cfr9I CCCGGG 1 cut(s) 132
CpoI CGGWCCG 1 cut(s) 166
CseI GACGC 2 cut(s) 202, 514
CsiI ACCWGGT 1 cut(s) 624
Csp6I GTAC 1 cut(s) 637
CspI CGGWCCG 1 cut(s) 166
CviAII CATG 3 cut(s) 551, 680, 710
CviQI GTAC 1 cut(s) 637
DdeI CTNAG 1 cut(s) 447
DpnI GATC 5 cut(s) 77, 119, 126, 219, 738
DpnII GATC 5 cut(s) 75, 117, 124, 217, 736
DrdI GACNNNNNNGTC 2 cut(s) 164, 374
DseDI GACNNNNNNGTC 2 cut(s) 164, 374
EciI GGCGGA 1 cut(s) 533
Eco130I CCWWGG 2 cut(s) 108, 732
Eco24I GRGCYC 1 cut(s) 709
Eco47I GGWCC 3 cut(s) 166, 622, 670
Eco57I CTGAAG 1 cut(s) 596
Eco88I CYCGRG 1 cut(s) 132
Eco91I GGTNACC 1 cut(s) 334
EcoO109I RGGNCCY 2 cut(s) 198, 622
EcoO65I GGTNACC 1 cut(s) 334
EcoRII CCWGG 3 cut(s) 618, 624, 672
EcoT14I CCWWGG 2 cut(s) 108, 732
EcoT38I GRGCYC 1 cut(s) 709
ErhI CCWWGG 2 cut(s) 108, 732
FaeI CATG 3 cut(s) 554, 683, 713
FaiI YATR 5 cut(s) 116, 552, 681, 711, 764
FaqI GGGAC 2 cut(s) 69, 130
FatI CATG 3 cut(s) 550, 679, 709
FauI CCCGC 1 cut(s) 579
FbaI TGATCA 1 cut(s) 117
Fnu4HI GCNGC 4 cut(s) 228, 315, 381, 509
FriOI GRGCYC 1 cut(s) 709
Fsp4HI GCNGC 4 cut(s) 228, 315, 381, 509
FspBI CTAG 1 cut(s) 692
GlaI GCGC 3 cut(s) 357, 483, 585
GluI GCNGC 4 cut(s) 228, 315, 381, 509
GsaI CCCAGC 1 cut(s) 600
HaeIII GGCC 6 cut(s) 137, 200, 269, 281, 473, 602
HapII CCGG 8 cut(s) 23, 133, 169, 192, 210, 275, 342, 768
HgaI GACGC 2 cut(s) 202, 514
HhaI GCGC 3 cut(s) 358, 484, 586
Hin1I GRCGYC 3 cut(s) 213, 252, 506
Hin1II CATG 3 cut(s) 554, 683, 713
Hin6I GCGC 3 cut(s) 356, 482, 584
HinP1I GCGC 3 cut(s) 356, 482, 584
HincII GTYRAC 1 cut(s) 43
HindII GTYRAC 1 cut(s) 43
HindIII AAGCTT 1 cut(s) 452
HinfI GANTC 3 cut(s) 60, 161, 345
HpaII CCGG 8 cut(s) 23, 133, 169, 192, 210, 275, 342, 768
HphI GGTGA 3 cut(s) 214, 328, 539
Hpy166II GTNNAC 3 cut(s) 43, 547, 670
Hpy188I TCNGA 2 cut(s) 450, 774
Hpy188III TCNNGA 3 cut(s) 73, 155, 710
Hpy8I GTNNAC 3 cut(s) 43, 547, 670
Hpy99I CGWCG 5 cut(s) 218, 254, 380, 436, 508
HpyAV CCTTC 3 cut(s) 76, 211, 798
HpyCH4III ACNGT 1 cut(s) 83
HpyCH4IV ACGT 2 cut(s) 237, 252
HpyCH4V TGCA 2 cut(s) 297, 754
HpyF10VI GCNNNNNNNGC 5 cut(s) 100, 197, 338, 389, 479
HpyF3I CTNAG 1 cut(s) 447
HpySE526I ACGT 2 cut(s) 237, 252
Hsp92I GRCGYC 3 cut(s) 213, 252, 506
Hsp92II CATG 3 cut(s) 554, 683, 713
HspAI GCGC 3 cut(s) 356, 482, 584
Ksp22I TGATCA 1 cut(s) 117
Kzo9I GATC 5 cut(s) 75, 117, 124, 217, 736
LmnI GCTCC 3 cut(s) 212, 289, 389
Lsp1109I GCAGC 1 cut(s) 214
LweI GCATC 1 cut(s) 741
MabI ACCWGGT 1 cut(s) 624
MaeI CTAG 1 cut(s) 692
MaeII ACGT 2 cut(s) 237, 252
MaeIII GTNAC 3 cut(s) 70, 334, 365
MalI GATC 5 cut(s) 77, 119, 126, 219, 738
MbiI CCGCTC 1 cut(s) 292
MboI GATC 5 cut(s) 75, 117, 124, 217, 736
MboII GAAGA 5 cut(s) 9, 589, 622, 760, 783
MflI RGATCY 1 cut(s) 124
MhlI GDGCHC 1 cut(s) 709
MlyI GAGTC 1 cut(s) 69
MmeI TCCRAC 1 cut(s) 752
MseI TTAA 3 cut(s) 651, 663, 744
MspA1I CMGCKG 1 cut(s) 227
MspCI CTTAAG 1 cut(s) 662
MspI CCGG 8 cut(s) 23, 133, 169, 192, 210, 275, 342, 768
MspR9I CCNGG 7 cut(s) 133, 134, 170, 275, 620, 626, 674
MvaI CCWGG 3 cut(s) 620, 626, 674
MvnI CGCG 5 cut(s) 48, 332, 356, 484, 586
MwoI GCNNNNNNNGC 5 cut(s) 100, 197, 338, 389, 479
NciI CCSGG 4 cut(s) 133, 134, 170, 275
NdeII GATC 5 cut(s) 75, 117, 124, 217, 736
NlaIII CATG 3 cut(s) 554, 683, 713
NlaIV GGNNCC 3 cut(s) 190, 472, 601
NmeAIII GCCGAG 1 cut(s) 256
NmuCI GTSAC 3 cut(s) 70, 334, 365
NspV TTCGAA 1 cut(s) 348
PagI TCATGA 1 cut(s) 709
PcsI WCGNNNNNNNCGW 2 cut(s) 45, 500
PfeI GAWTC 2 cut(s) 161, 345
PflFI GACNNNGTC 1 cut(s) 59
PflMI CCANNNNNTGG 2 cut(s) 556, 625
PkrI GCNGC 4 cut(s) 229, 316, 382, 510
PleI GAGTC 1 cut(s) 68
PpsI GAGTC 1 cut(s) 68
PpuMI RGGWCCY 1 cut(s) 622
PshAI GACNNNNGTC 1 cut(s) 675
PshBI ATTAAT 1 cut(s) 651
Psp5II RGGWCCY 1 cut(s) 622
Psp6I CCWGG 3 cut(s) 618, 624, 672
PspEI GGTNACC 1 cut(s) 334
PspFI CCCAGC 1 cut(s) 596
PspGI CCWGG 3 cut(s) 618, 624, 672
PspN4I GGNNCC 3 cut(s) 190, 472, 601
PspPI GGNCC 8 cut(s) 135, 166, 198, 280, 471, 600, 622, 670
PspPPI RGGWCCY 1 cut(s) 622
PstNI CAGNNNCTG 1 cut(s) 625
PsuI RGATCY 1 cut(s) 124
PsyI GACNNNGTC 1 cut(s) 59
RsaI GTAC 1 cut(s) 638
RsaNI GTAC 1 cut(s) 637
Rsr2I CGGWCCG 1 cut(s) 166
RsrII CGGWCCG 1 cut(s) 166
SaqAI TTAA 3 cut(s) 651, 663, 744
SatI GCNGC 4 cut(s) 228, 315, 381, 509
Sau3AI GATC 5 cut(s) 75, 117, 124, 217, 736
Sau96I GGNCC 8 cut(s) 135, 166, 198, 280, 471, 600, 622, 670
SchI GAGTC 1 cut(s) 69
ScrFI CCNGG 7 cut(s) 133, 134, 170, 275, 620, 626, 674
SduI GDGCHC 1 cut(s) 709
SexAI ACCWGGT 1 cut(s) 624
SfaNI GCATC 1 cut(s) 741
SfuI TTCGAA 1 cut(s) 348
SinI GGWCC 3 cut(s) 166, 622, 670
SmaI CCCGGG 1 cut(s) 134
SmlI CTYRAG 1 cut(s) 662
SmoI CTYRAG 1 cut(s) 662
SrfI GCCCGGGC 1 cut(s) 134
SspI AATATT 1 cut(s) 716
SspMI CTAG 1 cut(s) 692
StyD4I CCNGG 7 cut(s) 131, 132, 168, 273, 618, 624, 672
StyI CCWWGG 2 cut(s) 108, 732
TaaI ACNGT 1 cut(s) 83
TaiI ACGT 2 cut(s) 240, 255
TaqI TCGA 5 cut(s) 216, 264, 348, 402, 458
TaqII GACCGA 1 cut(s) 154
TauI GCSGC 3 cut(s) 317, 383, 511
TfiI GAWTC 2 cut(s) 161, 345
Tru1I TTAA 3 cut(s) 651, 663, 744
Tru9I TTAA 3 cut(s) 651, 663, 744
TseFI GTSAC 3 cut(s) 70, 334, 365
TseI GCWGC 1 cut(s) 227
Tsp45I GTSAC 3 cut(s) 70, 334, 365
TspDTI ATGAA 2 cut(s) 388, 726
TspGWI ACGGA 3 cut(s) 72, 137, 420
TspMI CCCGGG 1 cut(s) 132
Tth111I GACNNNGTC 1 cut(s) 59
Van91I CCANNNNNTGG 2 cut(s) 556, 625
Vha464I CTTAAG 1 cut(s) 662
VpaK11BI GGWCC 3 cut(s) 166, 622, 670
VspI ATTAAT 1 cut(s) 651
XmaI CCCGGG 1 cut(s) 132
XspI CTAG 1 cut(s) 692
ZraI GACGTC 1 cut(s) 253
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.