Rh4DG262600

enoyl-CoA hydratase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0012967
Physical Location & Seq
Forward (+)
48118823 .. 48121068
2246 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG262600.1

Sequence Viewer

Length: 804 bp
ATGGACCGGAAACAATCTTCGCCGGAAAATCTCATTCTCGTCGACCGCGAACCGGACGGAGTCGCCTTCGTGACGATCAATCGTCCCAAGTTGCTCAACTCGCTGACCAAGGCTATGATCACGGATCTCGCCCGGGCCATCAAGTCACTGAATCAAGACGATTCAGTCCGGGTCATTGTTCTGTCGGGTGCGGGTCGCGCCTTCTGCTCCGGCGTCGATCTCACCGCTGCCGAGGACGTTTTCAAAGGCGACGTCAAGGACATCGAGGCCGACCCGGTGGCCCAAATGGAGCGGTGCAAAAAGCCCATCATCGCCGCCATCAACGGCTTCGCGGTCACCGCCGGATTCGAAATCGCGCTCGCCTGCGACATCATCGTCGCCGCTAAAGGAGCGAAGTTCCTCGACACTCATGCCAGATTTGGGATATTTCCGTCGTGGGGTTTGTCTCAGAAGCTTTCGAGGATAATAGGGGCCAACAAAGCGCGTGAGGTGTCGCTGACGGCGACGCCGCTAACGGCGGAGGTGGGTGAGAGGTTGGGGTTTGTGAACCATGTTGTGGAGGAGAGTGAGTTGCTGAAGAAAGCACGGGAAGTCGCTGGGGCCATTGTGAAGAACAACCAGGACCTGGTGTTGAGGTACAAGGCTGTTATTAATGATGGGCTTAAGCTGGACCTGGGTCATGCTCTTACACTAGAAAAGGAGAGGGCTCATGAATATTACAATGGAATGACCAAGGATCAGTTTAAGAAGATGCAAGACTTTATAGCCGGTCGGAGTTCTAAAAAGCCTTCTTCCAAGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ECH_1

Protein Analysis

267

Amino Acids

29.11

Weight (kDa)

9.01

Isoelectric Point (pI)

24.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ECH_1 PF00378 18 - 242 5.4e-52 Enoyl-CoA hydratase/isomerase
ECH_2 PF16113 22 - 198 4.1e-26 Enoyl-CoA hydratase/isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012967)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G16210
fragaria_vesca FvH4_4g19880
malus_domestica MD16G1152800.v1.1
prunus_persica Prupe.1G116000_v2.0.a1
pyrus_communis pycom16g12990
rosa_chinensis RchiOBHm_Chr4g0425501 RchiOBHm_Chr4g0425511
rosa_laevigata RLG00000007359 RLG00000007363 RLG00000007365 RLG00000007367
rosa_multiflora Rmu_sc0001900.1_g000011
rosa_roxburghii Rroxscaffold_5G00367450
rosa_rugosa Rorug04G0203600
rosa_samantha Rh4AG260600 Rh4BG266300 Rh4DG262600
rosa_wichuraiana Rw4G022560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 164, 374
AatII GACGTC 1 cut(s) 255
AccB7I CCANNNNNTGG 2 cut(s) 556, 625
AccBSI CCGCTC 1 cut(s) 292
AccI GTMKAC 1 cut(s) 42
AccII CGCG 5 cut(s) 48, 198, 332, 356, 484
AclWI GGATC 2 cut(s) 132, 744
AcuI CTGAAG 1 cut(s) 596
AcyI GRCGYC 3 cut(s) 213, 252, 506
AfaI GTAC 1 cut(s) 638
AfiI CCNNNNNNNGG 5 cut(s) 52, 420, 437, 556, 625
AflII CTTAAG 1 cut(s) 662
AgsI TTSAA 1 cut(s) 244
AjnI CCWGG 3 cut(s) 618, 624, 672
AluBI AGCT 2 cut(s) 454, 667
AluI AGCT 2 cut(s) 454, 667
Alw26I GTCTC 1 cut(s) 450
AlwI GGATC 2 cut(s) 132, 744
AlwNI CAGNNNCTG 1 cut(s) 625
Ama87I CYCGRG 1 cut(s) 132
AoxI GGCC 5 cut(s) 135, 267, 279, 471, 600
ApeKI GCWGC 1 cut(s) 227
ArsI GACNNNNNNTTYG 2 cut(s) 237, 269
AseI ATTAAT 1 cut(s) 651
AspLEI GCGC 3 cut(s) 200, 358, 484
AspS9I GGNCC 7 cut(s) 4, 135, 280, 471, 600, 622, 670
AsuC2I CCSGG 4 cut(s) 133, 134, 170, 275
AsuHPI GGTGA 3 cut(s) 214, 328, 539
AsuII TTCGAA 1 cut(s) 348
AvaI CYCGRG 1 cut(s) 132
AvaII GGWCC 3 cut(s) 4, 622, 670
BanII GRGCYC 1 cut(s) 709
BbvI GCAGC 1 cut(s) 214
BccI CCATC 4 cut(s) 146, 314, 326, 650
BceAI ACGGC 3 cut(s) 340, 516, 531
BcgI CGANNNNNNTGC 2 cut(s) 392, 426
BciT130I CCWGG 3 cut(s) 620, 626, 674
BclI TGATCA 1 cut(s) 117
BcnI CCSGG 4 cut(s) 133, 134, 170, 275
BcoDI GTCTC 1 cut(s) 450
BfaI CTAG 1 cut(s) 692
BfrI CTTAAG 1 cut(s) 662
BisI GCNGC 4 cut(s) 228, 315, 381, 509
BlsI GCNGC 4 cut(s) 229, 316, 382, 510
Bme1390I CCNGG 7 cut(s) 133, 134, 170, 275, 620, 626, 674
Bme18I GGWCC 3 cut(s) 4, 622, 670
BmeT110I CYCGRG 1 cut(s) 132
BmgT120I GGNCC 7 cut(s) 4, 135, 280, 471, 600, 622, 670
BmiI GGNNCC 2 cut(s) 472, 601
BmrFI CCNGG 7 cut(s) 133, 134, 170, 275, 620, 626, 674
BmsI GCATC 1 cut(s) 741
BoxI GACNNNNGTC 1 cut(s) 675
Bpu14I TTCGAA 1 cut(s) 348
BpuMI CCSGG 4 cut(s) 133, 134, 170, 275
BsaHI GRCGYC 3 cut(s) 213, 252, 506
BsaJI CCNNGG 5 cut(s) 108, 132, 231, 673, 732
BsaWI WCCGGW 2 cut(s) 6, 52
BsaXI ACNNNNNCTCC 2 cut(s) 554, 584
Bsc4I CCNNNNNNNGG 5 cut(s) 52, 420, 437, 556, 625
Bse118I RCCGGY 1 cut(s) 767
BseBI CCWGG 3 cut(s) 620, 626, 674
BseDI CCNNGG 5 cut(s) 108, 132, 231, 673, 732
BseLI CCNNNNNNNGG 5 cut(s) 52, 420, 437, 556, 625
BseMII CTCAG 1 cut(s) 461
BseRI GAGGAG 1 cut(s) 575
BseXI GCAGC 1 cut(s) 214
BseYI CCCAGC 1 cut(s) 596
Bsh1236I CGCG 5 cut(s) 48, 198, 332, 356, 484
Bsh1285I CGRYCG 2 cut(s) 46, 772
BshFI GGCC 5 cut(s) 137, 269, 281, 473, 602
BsiEI CGRYCG 2 cut(s) 46, 772
BsiHKCI CYCGRG 1 cut(s) 132
BsiSI CCGG 9 cut(s) 7, 23, 53, 133, 169, 210, 275, 342, 768
BslFI GGGAC 1 cut(s) 69
BslI CCNNNNNNNGG 5 cut(s) 52, 420, 437, 556, 625
BsmAI GTCTC 1 cut(s) 450
BsmFI GGGAC 1 cut(s) 69
BsnI GGCC 5 cut(s) 137, 269, 281, 473, 602
BsoBI CYCGRG 1 cut(s) 132
Bsp119I TTCGAA 1 cut(s) 348
Bsp1286I GDGCHC 1 cut(s) 709
Bsp143I GATC 5 cut(s) 75, 117, 124, 217, 736
BspANI GGCC 5 cut(s) 137, 269, 281, 473, 602
BspCNI CTCAG 1 cut(s) 460
BspFNI CGCG 5 cut(s) 48, 198, 332, 356, 484
BspHI TCATGA 1 cut(s) 709
BspLI GGNNCC 2 cut(s) 472, 601
BspPI GGATC 2 cut(s) 132, 744
BspT104I TTCGAA 1 cut(s) 348
BspTI CTTAAG 1 cut(s) 662
BsrBI CCGCTC 1 cut(s) 292
BsrFI RCCGGY 1 cut(s) 767
BssAI RCCGGY 1 cut(s) 767
BssECI CCNNGG 5 cut(s) 108, 132, 231, 673, 732
BssMI GATC 5 cut(s) 75, 117, 124, 217, 736
BssNI GRCGYC 3 cut(s) 213, 252, 506
BssT1I CCWWGG 2 cut(s) 108, 732
Bst2UI CCWGG 3 cut(s) 620, 626, 674
BstACI GRCGYC 3 cut(s) 213, 252, 506
BstAFI CTTAAG 1 cut(s) 662
BstBI TTCGAA 1 cut(s) 348
BstC8I GCNNGC 2 cut(s) 360, 364
BstDEI CTNAG 1 cut(s) 447
BstEII GGTNACC 1 cut(s) 334
BstFNI CGCG 5 cut(s) 48, 198, 332, 356, 484
BstHHI GCGC 3 cut(s) 200, 358, 484
BstKTI GATC 5 cut(s) 78, 120, 127, 220, 739
BstMAI GTCTC 1 cut(s) 450
BstMBI GATC 5 cut(s) 75, 117, 124, 217, 736
BstMCI CGRYCG 2 cut(s) 46, 772
BstMWI GCNNNNNNNGC 6 cut(s) 100, 197, 204, 338, 389, 479
BstNI CCWGG 3 cut(s) 620, 626, 674
BstPAI GACNNNNGTC 1 cut(s) 675
BstPI GGTNACC 1 cut(s) 334
BstSCI CCNGG 7 cut(s) 131, 132, 168, 273, 618, 624, 672
BstUI CGCG 5 cut(s) 48, 198, 332, 356, 484
BstV1I GCAGC 1 cut(s) 214
BstX2I RGATCY 1 cut(s) 124
BstYI RGATCY 1 cut(s) 124
BsuRI GGCC 5 cut(s) 137, 269, 281, 473, 602
BtgZI GCGATG 1 cut(s) 295
BtsIMutI CAGTG 1 cut(s) 146
Cac8I GCNNGC 2 cut(s) 360, 364
CaiI CAGNNNCTG 1 cut(s) 625
CciI TCATGA 1 cut(s) 709
CfoI GCGC 3 cut(s) 200, 358, 484
Cfr10I RCCGGY 1 cut(s) 767
Cfr13I GGNCC 7 cut(s) 4, 135, 280, 471, 600, 622, 670
Cfr9I CCCGGG 1 cut(s) 132
CseI GACGC 2 cut(s) 202, 514
CsiI ACCWGGT 1 cut(s) 624
Csp6I GTAC 1 cut(s) 637
CviAII CATG 4 cut(s) 410, 551, 680, 710
CviQI GTAC 1 cut(s) 637
DdeI CTNAG 1 cut(s) 447
DpnI GATC 5 cut(s) 77, 119, 126, 219, 738
DpnII GATC 5 cut(s) 75, 117, 124, 217, 736
DrdI GACNNNNNNGTC 2 cut(s) 164, 374
DseDI GACNNNNNNGTC 2 cut(s) 164, 374
EciI GGCGGA 1 cut(s) 533
Eco130I CCWWGG 2 cut(s) 108, 732
Eco24I GRGCYC 1 cut(s) 709
Eco47I GGWCC 3 cut(s) 4, 622, 670
Eco57I CTGAAG 1 cut(s) 596
Eco88I CYCGRG 1 cut(s) 132
Eco91I GGTNACC 1 cut(s) 334
EcoO109I RGGNCCY 1 cut(s) 622
EcoO65I GGTNACC 1 cut(s) 334
EcoRII CCWGG 3 cut(s) 618, 624, 672
EcoT14I CCWWGG 2 cut(s) 108, 732
EcoT38I GRGCYC 1 cut(s) 709
ErhI CCWWGG 2 cut(s) 108, 732
FaeI CATG 4 cut(s) 413, 554, 683, 713
FaiI YATR 6 cut(s) 116, 411, 552, 681, 711, 764
FaqI GGGAC 1 cut(s) 69
FatI CATG 4 cut(s) 409, 550, 679, 709
FauI CCCGC 1 cut(s) 184
FbaI TGATCA 1 cut(s) 117
FblI GTMKAC 1 cut(s) 42
Fnu4HI GCNGC 4 cut(s) 228, 315, 381, 509
FriOI GRGCYC 1 cut(s) 709
Fsp4HI GCNGC 4 cut(s) 228, 315, 381, 509
FspBI CTAG 1 cut(s) 692
GlaI GCGC 3 cut(s) 199, 357, 483
GluI GCNGC 4 cut(s) 228, 315, 381, 509
GsaI CCCAGC 1 cut(s) 600
HaeIII GGCC 5 cut(s) 137, 269, 281, 473, 602
HapII CCGG 9 cut(s) 7, 23, 53, 133, 169, 210, 275, 342, 768
HgaI GACGC 2 cut(s) 202, 514
HhaI GCGC 3 cut(s) 200, 358, 484
Hin1I GRCGYC 3 cut(s) 213, 252, 506
Hin1II CATG 4 cut(s) 413, 554, 683, 713
Hin6I GCGC 3 cut(s) 198, 356, 482
HinP1I GCGC 3 cut(s) 198, 356, 482
HincII GTYRAC 1 cut(s) 43
HindII GTYRAC 1 cut(s) 43
HindIII AAGCTT 1 cut(s) 452
HinfI GANTC 4 cut(s) 60, 151, 161, 345
HpaII CCGG 9 cut(s) 7, 23, 53, 133, 169, 210, 275, 342, 768
HphI GGTGA 3 cut(s) 214, 328, 539
Hpy166II GTNNAC 2 cut(s) 43, 547
Hpy188I TCNGA 2 cut(s) 450, 774
Hpy188III TCNNGA 3 cut(s) 70, 155, 710
Hpy8I GTNNAC 2 cut(s) 43, 547
Hpy99I CGWCG 6 cut(s) 44, 218, 254, 380, 436, 508
HpyAV CCTTC 3 cut(s) 76, 211, 798
HpyCH4IV ACGT 2 cut(s) 237, 252
HpyCH4V TGCA 2 cut(s) 297, 754
HpyF10VI GCNNNNNNNGC 6 cut(s) 100, 197, 204, 338, 389, 479
HpyF3I CTNAG 1 cut(s) 447
HpySE526I ACGT 2 cut(s) 237, 252
Hsp92I GRCGYC 3 cut(s) 213, 252, 506
Hsp92II CATG 4 cut(s) 413, 554, 683, 713
HspAI GCGC 3 cut(s) 198, 356, 482
Ksp22I TGATCA 1 cut(s) 117
Kzo9I GATC 5 cut(s) 75, 117, 124, 217, 736
LmnI GCTCC 3 cut(s) 212, 289, 389
Lsp1109I GCAGC 1 cut(s) 214
LweI GCATC 1 cut(s) 741
MabI ACCWGGT 1 cut(s) 624
MaeI CTAG 1 cut(s) 692
MaeII ACGT 2 cut(s) 237, 252
MaeIII GTNAC 3 cut(s) 70, 144, 334
MalI GATC 5 cut(s) 77, 119, 126, 219, 738
MbiI CCGCTC 1 cut(s) 292
MboI GATC 5 cut(s) 75, 117, 124, 217, 736
MboII GAAGA 5 cut(s) 9, 589, 622, 760, 783
MflI RGATCY 1 cut(s) 124
MhlI GDGCHC 1 cut(s) 709
MlyI GAGTC 1 cut(s) 69
MmeI TCCRAC 1 cut(s) 752
MseI TTAA 3 cut(s) 651, 663, 744
MspA1I CMGCKG 1 cut(s) 227
MspCI CTTAAG 1 cut(s) 662
MspI CCGG 9 cut(s) 7, 23, 53, 133, 169, 210, 275, 342, 768
MspR9I CCNGG 7 cut(s) 133, 134, 170, 275, 620, 626, 674
MvaI CCWGG 3 cut(s) 620, 626, 674
MvnI CGCG 5 cut(s) 48, 198, 332, 356, 484
MwoI GCNNNNNNNGC 6 cut(s) 100, 197, 204, 338, 389, 479
NciI CCSGG 4 cut(s) 133, 134, 170, 275
NdeII GATC 5 cut(s) 75, 117, 124, 217, 736
NlaIII CATG 4 cut(s) 413, 554, 683, 713
NlaIV GGNNCC 2 cut(s) 472, 601
NmeAIII GCCGAG 1 cut(s) 256
NmuCI GTSAC 3 cut(s) 70, 144, 334
NspV TTCGAA 1 cut(s) 348
PagI TCATGA 1 cut(s) 709
PcsI WCGNNNNNNNCGW 2 cut(s) 45, 500
PfeI GAWTC 3 cut(s) 151, 161, 345
PflFI GACNNNGTC 1 cut(s) 59
PflMI CCANNNNNTGG 2 cut(s) 556, 625
PkrI GCNGC 4 cut(s) 229, 316, 382, 510
PleI GAGTC 1 cut(s) 68
PpsI GAGTC 1 cut(s) 68
PpuMI RGGWCCY 1 cut(s) 622
PshAI GACNNNNGTC 1 cut(s) 675
PshBI ATTAAT 1 cut(s) 651
Psp5II RGGWCCY 1 cut(s) 622
Psp6I CCWGG 3 cut(s) 618, 624, 672
PspEI GGTNACC 1 cut(s) 334
PspFI CCCAGC 1 cut(s) 596
PspGI CCWGG 3 cut(s) 618, 624, 672
PspN4I GGNNCC 2 cut(s) 472, 601
PspPI GGNCC 7 cut(s) 4, 135, 280, 471, 600, 622, 670
PspPPI RGGWCCY 1 cut(s) 622
PstNI CAGNNNCTG 1 cut(s) 625
PsuI RGATCY 1 cut(s) 124
PsyI GACNNNGTC 1 cut(s) 59
RsaI GTAC 1 cut(s) 638
RsaNI GTAC 1 cut(s) 637
SalI GTCGAC 1 cut(s) 41
SaqAI TTAA 3 cut(s) 651, 663, 744
SatI GCNGC 4 cut(s) 228, 315, 381, 509
Sau3AI GATC 5 cut(s) 75, 117, 124, 217, 736
Sau96I GGNCC 7 cut(s) 4, 135, 280, 471, 600, 622, 670
SchI GAGTC 1 cut(s) 69
ScrFI CCNGG 7 cut(s) 133, 134, 170, 275, 620, 626, 674
SduI GDGCHC 1 cut(s) 709
SexAI ACCWGGT 1 cut(s) 624
SfaNI GCATC 1 cut(s) 741
SfuI TTCGAA 1 cut(s) 348
SinI GGWCC 3 cut(s) 4, 622, 670
SmaI CCCGGG 1 cut(s) 134
SmlI CTYRAG 1 cut(s) 662
SmoI CTYRAG 1 cut(s) 662
SrfI GCCCGGGC 1 cut(s) 134
SspI AATATT 1 cut(s) 716
SspMI CTAG 1 cut(s) 692
StyD4I CCNGG 7 cut(s) 131, 132, 168, 273, 618, 624, 672
StyI CCWWGG 2 cut(s) 108, 732
TaiI ACGT 2 cut(s) 240, 255
TaqI TCGA 6 cut(s) 42, 216, 264, 348, 402, 458
TauI GCSGC 3 cut(s) 317, 383, 511
TfiI GAWTC 3 cut(s) 151, 161, 345
Tru1I TTAA 3 cut(s) 651, 663, 744
Tru9I TTAA 3 cut(s) 651, 663, 744
TscAI CASTG 1 cut(s) 153
TseFI GTSAC 3 cut(s) 70, 144, 334
TseI GCWGC 1 cut(s) 227
Tsp45I GTSAC 3 cut(s) 70, 144, 334
TspDTI ATGAA 1 cut(s) 726
TspGWI ACGGA 3 cut(s) 72, 137, 420
TspMI CCCGGG 1 cut(s) 132
TspRI CASTG 1 cut(s) 153
Tth111I GACNNNGTC 1 cut(s) 59
Van91I CCANNNNNTGG 2 cut(s) 556, 625
Vha464I CTTAAG 1 cut(s) 662
VpaK11BI GGWCC 3 cut(s) 4, 622, 670
VspI ATTAAT 1 cut(s) 651
XmaI CCCGGG 1 cut(s) 132
XmiI GTMKAC 1 cut(s) 42
XspI CTAG 1 cut(s) 692
ZraI GACGTC 1 cut(s) 253
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.