RLG00000007363

enoyl-CoA hydratase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0012967
Physical Location & Seq
Reverse (-)
16140167 .. 16140889
723 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000007363

Sequence Viewer

Length: 633 bp
ATGGACGGGAAACAATCTTCGCCGGAAAATCTCATTCTCGTCAACCGCGAACCGGACGGAATCGCTTTCGTCAGGATCAACCGTCCCAAGTCGCTTAACTTGCTGACCAAGGCTATGATCATGGATCTCGCCCGGGCCATCAAGTCCCTGAATCAAGACGATTCGGTCCGGGCCATTATTCTGTCGGGTACGGGTCGGGCCTTCTGCTCGGGCGTCAATCTCACCGCGGCCGAGGACGTTTTCAAAGGCGACGTCAAGGACATCAAGACCGCGGGGGCCCAAATAGAGCTATGCAAAAAGCCCATCATCACCGCCATCCACGGCTTCGCGGTAGCCGGCGGATTTCGAAATCGCGATCGGTTGCGACATCCTAAAGGAGCGAAGTTCATCGATACTCATGCCACATTTGGAGTACTTCCGTCGCTGGGTTTGTCTCAGAAGCTTTCGAGGATAATAGGGGCCAACAAAGCGCGTGAGATGTCACTGACGGCGACGCCGCTAACGGCGGAGGTGGTTGAGAGGTTGGGGTTAGTGAACCGTGTTGTGGAGGAGAGTGAGTTGCTGAAGAAAGCGCGGGAAATAGCAGAGGCGATTGTGAAGAATAATAACCAGGACTTGGTGTTGAGGTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ECH_1

Protein Analysis

211

Amino Acids

22.9

Weight (kDa)

9.76

Isoelectric Point (pI)

32.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ECH_1 PF00378 18 - 203 1.2e-35 Enoyl-CoA hydratase/isomerase
ECH_2 PF16113 22 - 197 4.5e-18 Enoyl-CoA hydratase/isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012967)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G16210
fragaria_vesca FvH4_4g19880
malus_domestica MD16G1152800.v1.1
prunus_persica Prupe.1G116000_v2.0.a1
pyrus_communis pycom16g12990
rosa_chinensis RchiOBHm_Chr4g0425501 RchiOBHm_Chr4g0425511
rosa_laevigata RLG00000007359 RLG00000007363 RLG00000007365 RLG00000007367
rosa_multiflora Rmu_sc0001900.1_g000011
rosa_roxburghii Rroxscaffold_5G00367450
rosa_rugosa Rorug04G0203600
rosa_samantha Rh4AG260600 Rh4BG266300 Rh4DG262600
rosa_wichuraiana Rw4G022560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 164
AatII GACGTC 1 cut(s) 255
AccB7I CCANNNNNTGG 1 cut(s) 616
AccII CGCG 7 cut(s) 48, 227, 272, 329, 354, 472, 574
AclWI GGATC 2 cut(s) 83, 132
AcoI YGGCCR 1 cut(s) 228
AcuI CTGAAG 1 cut(s) 584
AcyI GRCGYC 3 cut(s) 213, 252, 494
AfaI GTAC 3 cut(s) 190, 414, 629
AfiI CCNNNNNNNGG 4 cut(s) 52, 425, 544, 616
AgsI TTSAA 1 cut(s) 244
AjnI CCWGG 1 cut(s) 609
AluBI AGCT 2 cut(s) 289, 442
AluI AGCT 2 cut(s) 289, 442
Alw26I GTCTC 1 cut(s) 438
AlwI GGATC 2 cut(s) 83, 132
Ama87I CYCGRG 2 cut(s) 132, 208
AoxI GGCC 6 cut(s) 135, 171, 198, 228, 276, 459
ApaI GGGCCC 1 cut(s) 280
ArsI GACNNNNNNTTYG 2 cut(s) 237, 269
AspLEI GCGC 2 cut(s) 472, 574
AspS9I GGNCC 7 cut(s) 135, 166, 171, 198, 276, 277, 459
AsuC2I CCSGG 3 cut(s) 133, 134, 170
AsuHPI GGTGA 2 cut(s) 214, 301
AsuII TTCGAA 1 cut(s) 346
AvaI CYCGRG 2 cut(s) 132, 208
AvaII GGWCC 1 cut(s) 166
BaeGI GKGCMC 1 cut(s) 280
BanII GRGCYC 1 cut(s) 280
BccI CCATC 3 cut(s) 146, 311, 323
BceAI ACGGC 3 cut(s) 337, 504, 519
BcgI CGANNNNNNTGC 2 cut(s) 380, 414
BciT130I CCWGG 1 cut(s) 611
BclI TGATCA 1 cut(s) 117
BcnI CCSGG 3 cut(s) 133, 134, 170
BcoDI GTCTC 1 cut(s) 438
BfaI CTAG 1 cut(s) 631
BisI GCNGC 2 cut(s) 228, 497
BlsI GCNGC 2 cut(s) 229, 498
BmcAI AGTACT 1 cut(s) 414
Bme1390I CCNGG 4 cut(s) 133, 134, 170, 611
Bme18I GGWCC 1 cut(s) 166
BmeT110I CYCGRG 2 cut(s) 132, 208
BmgT120I GGNCC 7 cut(s) 135, 166, 171, 198, 276, 277, 459
BmiI GGNNCC 3 cut(s) 277, 278, 460
BmrFI CCNGG 4 cut(s) 133, 134, 170, 611
Bpu14I TTCGAA 1 cut(s) 346
BpuMI CCSGG 3 cut(s) 133, 134, 170
Bsa29I ATCGAT 1 cut(s) 390
BsaHI GRCGYC 3 cut(s) 213, 252, 494
BsaJI CCNNGG 6 cut(s) 108, 132, 225, 231, 270, 319
BsaWI WCCGGW 1 cut(s) 52
BsaXI ACNNNNNCTCC 2 cut(s) 542, 572
Bsc4I CCNNNNNNNGG 4 cut(s) 52, 425, 544, 616
Bse118I RCCGGY 1 cut(s) 335
BseBI CCWGG 1 cut(s) 611
BseCI ATCGAT 1 cut(s) 390
BseDI CCNNGG 6 cut(s) 108, 132, 225, 231, 270, 319
BseGI GGATG 2 cut(s) 315, 367
BseLI CCNNNNNNNGG 4 cut(s) 52, 425, 544, 616
BseMII CTCAG 1 cut(s) 449
BseRI GAGGAG 1 cut(s) 563
BseSI GKGCMC 1 cut(s) 280
BseX3I CGGCCG 1 cut(s) 228
BseYI CCCAGC 1 cut(s) 424
Bsh1236I CGCG 7 cut(s) 48, 227, 272, 329, 354, 472, 574
Bsh1285I CGRYCG 2 cut(s) 231, 358
BshFI GGCC 6 cut(s) 137, 173, 200, 230, 278, 461
BshVI ATCGAT 1 cut(s) 390
BsiEI CGRYCG 2 cut(s) 231, 358
BsiHKCI CYCGRG 2 cut(s) 132, 208
BsiSI CCGG 5 cut(s) 23, 53, 133, 169, 336
BslFI GGGAC 2 cut(s) 69, 130
BslI CCNNNNNNNGG 4 cut(s) 52, 425, 544, 616
BsmAI GTCTC 1 cut(s) 438
BsmFI GGGAC 2 cut(s) 69, 130
BsnI GGCC 6 cut(s) 137, 173, 200, 230, 278, 461
BsoBI CYCGRG 2 cut(s) 132, 208
Bsp119I TTCGAA 1 cut(s) 346
Bsp120I GGGCCC 1 cut(s) 276
Bsp1286I GDGCHC 1 cut(s) 280
Bsp143I GATC 4 cut(s) 75, 117, 124, 355
Bsp68I TCGCGA 1 cut(s) 354
BspANI GGCC 6 cut(s) 137, 173, 200, 230, 278, 461
BspCNI CTCAG 1 cut(s) 448
BspDI ATCGAT 1 cut(s) 390
BspFNI CGCG 7 cut(s) 48, 227, 272, 329, 354, 472, 574
BspLI GGNNCC 3 cut(s) 277, 278, 460
BspPI GGATC 2 cut(s) 83, 132
BspT104I TTCGAA 1 cut(s) 346
BsrFI RCCGGY 1 cut(s) 335
BssAI RCCGGY 1 cut(s) 335
BssECI CCNNGG 6 cut(s) 108, 132, 225, 231, 270, 319
BssMI GATC 4 cut(s) 75, 117, 124, 355
BssNI GRCGYC 3 cut(s) 213, 252, 494
BssT1I CCWWGG 1 cut(s) 108
Bst2UI CCWGG 1 cut(s) 611
Bst4CI ACNGT 2 cut(s) 83, 539
BstACI GRCGYC 3 cut(s) 213, 252, 494
BstBI TTCGAA 1 cut(s) 346
BstC8I GCNNGC 1 cut(s) 337
BstDEI CTNAG 1 cut(s) 435
BstDSI CCRYGG 3 cut(s) 225, 270, 319
BstF5I GGATG 2 cut(s) 315, 367
BstFNI CGCG 7 cut(s) 48, 227, 272, 329, 354, 472, 574
BstHHI GCGC 2 cut(s) 472, 574
BstKTI GATC 4 cut(s) 78, 120, 127, 358
BstMAI GTCTC 1 cut(s) 438
BstMBI GATC 4 cut(s) 75, 117, 124, 355
BstMCI CGRYCG 2 cut(s) 231, 358
BstMWI GCNNNNNNNGC 2 cut(s) 100, 467
BstNI CCWGG 1 cut(s) 611
BstSCI CCNGG 4 cut(s) 131, 132, 168, 609
BstSLI GKGCMC 1 cut(s) 280
BstUI CGCG 7 cut(s) 48, 227, 272, 329, 354, 472, 574
BstX2I RGATCY 1 cut(s) 124
BstYI RGATCY 1 cut(s) 124
BstZI CGGCCG 1 cut(s) 228
Bsu15I ATCGAT 1 cut(s) 390
BsuRI GGCC 6 cut(s) 137, 173, 200, 230, 278, 461
BsuTUI ATCGAT 1 cut(s) 390
BtgI CCRYGG 3 cut(s) 225, 270, 319
BtsCI GGATG 2 cut(s) 315, 367
BtsIMutI CAGTG 1 cut(s) 482
BtuMI TCGCGA 1 cut(s) 354
Cac8I GCNNGC 1 cut(s) 337
CfoI GCGC 2 cut(s) 472, 574
Cfr10I RCCGGY 1 cut(s) 335
Cfr13I GGNCC 7 cut(s) 135, 166, 171, 198, 276, 277, 459
Cfr42I CCGCGG 2 cut(s) 228, 273
Cfr9I CCCGGG 1 cut(s) 132
ClaI ATCGAT 1 cut(s) 390
CpoI CGGWCCG 1 cut(s) 166
CseI GACGC 2 cut(s) 202, 502
Csp6I GTAC 3 cut(s) 189, 413, 628
CspI CGGWCCG 1 cut(s) 166
CviAII CATG 2 cut(s) 121, 398
CviQI GTAC 3 cut(s) 189, 413, 628
DdeI CTNAG 1 cut(s) 435
DpnI GATC 4 cut(s) 77, 119, 126, 357
DpnII GATC 4 cut(s) 75, 117, 124, 355
DrdI GACNNNNNNGTC 1 cut(s) 164
DseDI GACNNNNNNGTC 1 cut(s) 164
EaeI YGGCCR 1 cut(s) 228
EagI CGGCCG 1 cut(s) 228
EciI GGCGGA 2 cut(s) 354, 521
EclXI CGGCCG 1 cut(s) 228
Eco130I CCWWGG 1 cut(s) 108
Eco24I GRGCYC 1 cut(s) 280
Eco47I GGWCC 1 cut(s) 166
Eco52I CGGCCG 1 cut(s) 228
Eco57I CTGAAG 1 cut(s) 584
Eco88I CYCGRG 2 cut(s) 132, 208
EcoO109I RGGNCCY 1 cut(s) 276
EcoRII CCWGG 1 cut(s) 609
EcoT14I CCWWGG 1 cut(s) 108
EcoT38I GRGCYC 1 cut(s) 280
ErhI CCWWGG 1 cut(s) 108
FaeI CATG 2 cut(s) 124, 401
FaiI YATR 4 cut(s) 116, 122, 292, 399
FaqI GGGAC 2 cut(s) 69, 130
FatI CATG 2 cut(s) 120, 397
FauI CCCGC 2 cut(s) 265, 567
FbaI TGATCA 1 cut(s) 117
Fnu4HI GCNGC 2 cut(s) 228, 497
FokI GGATG 2 cut(s) 302, 354
FriOI GRGCYC 1 cut(s) 280
Fsp4HI GCNGC 2 cut(s) 228, 497
FspBI CTAG 1 cut(s) 631
GlaI GCGC 2 cut(s) 471, 573
GluI GCNGC 2 cut(s) 228, 497
GsaI CCCAGC 1 cut(s) 428
HaeIII GGCC 6 cut(s) 137, 173, 200, 230, 278, 461
HapII CCGG 5 cut(s) 23, 53, 133, 169, 336
HgaI GACGC 2 cut(s) 202, 502
HhaI GCGC 2 cut(s) 472, 574
Hin1I GRCGYC 3 cut(s) 213, 252, 494
Hin1II CATG 2 cut(s) 124, 401
Hin6I GCGC 2 cut(s) 470, 572
HinP1I GCGC 2 cut(s) 470, 572
HincII GTYRAC 1 cut(s) 43
HindII GTYRAC 1 cut(s) 43
HindIII AAGCTT 1 cut(s) 440
HinfI GANTC 3 cut(s) 60, 151, 161
HpaII CCGG 5 cut(s) 23, 53, 133, 169, 336
HphI GGTGA 2 cut(s) 214, 301
Hpy166II GTNNAC 2 cut(s) 43, 535
Hpy188I TCNGA 1 cut(s) 438
Hpy188III TCNNGA 4 cut(s) 73, 155, 265, 353
Hpy8I GTNNAC 2 cut(s) 43, 535
Hpy99I CGWCG 3 cut(s) 254, 424, 496
HpyAV CCTTC 1 cut(s) 211
HpyCH4III ACNGT 2 cut(s) 83, 539
HpyCH4IV ACGT 2 cut(s) 237, 252
HpyCH4V TGCA 1 cut(s) 294
HpyF10VI GCNNNNNNNGC 2 cut(s) 100, 467
HpyF3I CTNAG 1 cut(s) 435
HpySE526I ACGT 2 cut(s) 237, 252
Hsp92I GRCGYC 3 cut(s) 213, 252, 494
Hsp92II CATG 2 cut(s) 124, 401
HspAI GCGC 2 cut(s) 470, 572
KroI GCCGGC 1 cut(s) 335
KroNI GCCGGC 1 cut(s) 337
Ksp22I TGATCA 1 cut(s) 117
KspI CCGCGG 2 cut(s) 228, 273
Kzo9I GATC 4 cut(s) 75, 117, 124, 355
LmnI GCTCC 1 cut(s) 377
MaeI CTAG 1 cut(s) 631
MaeII ACGT 2 cut(s) 237, 252
MaeIII GTNAC 1 cut(s) 480
MalI GATC 4 cut(s) 77, 119, 126, 357
MboI GATC 4 cut(s) 75, 117, 124, 355
MboII GAAGA 3 cut(s) 9, 577, 610
MflI RGATCY 1 cut(s) 124
MhlI GDGCHC 1 cut(s) 280
MnlI CCTC 7 cut(s) 226, 441, 502, 513, 541, 580, 618
MroNI GCCGGC 1 cut(s) 335
MseI TTAA 1 cut(s) 96
MspA1I CMGCKG 2 cut(s) 227, 272
MspI CCGG 5 cut(s) 23, 53, 133, 169, 336
MspR9I CCNGG 4 cut(s) 133, 134, 170, 611
MvaI CCWGG 1 cut(s) 611
MvnI CGCG 7 cut(s) 48, 227, 272, 329, 354, 472, 574
MwoI GCNNNNNNNGC 2 cut(s) 100, 467
NaeI GCCGGC 1 cut(s) 337
NciI CCSGG 3 cut(s) 133, 134, 170
NdeII GATC 4 cut(s) 75, 117, 124, 355
NgoMIV GCCGGC 1 cut(s) 335
NlaIII CATG 2 cut(s) 124, 401
NlaIV GGNNCC 3 cut(s) 277, 278, 460
NmeAIII GCCGAG 1 cut(s) 256
NmuCI GTSAC 1 cut(s) 480
NruI TCGCGA 1 cut(s) 354
NspV TTCGAA 1 cut(s) 346
PcsI WCGNNNNNNNCGW 1 cut(s) 45
PdiI GCCGGC 1 cut(s) 337
PfeI GAWTC 3 cut(s) 60, 151, 161
PflMI CCANNNNNTGG 1 cut(s) 616
PkrI GCNGC 2 cut(s) 229, 498
Ple19I CGATCG 1 cut(s) 358
Psp6I CCWGG 1 cut(s) 609
PspFI CCCAGC 1 cut(s) 424
PspGI CCWGG 1 cut(s) 609
PspN4I GGNNCC 3 cut(s) 277, 278, 460
PspOMI GGGCCC 1 cut(s) 276
PspPI GGNCC 7 cut(s) 135, 166, 171, 198, 276, 277, 459
PsuI RGATCY 1 cut(s) 124
PvuI CGATCG 1 cut(s) 358
RruI TCGCGA 1 cut(s) 354
RsaI GTAC 3 cut(s) 190, 414, 629
RsaNI GTAC 3 cut(s) 189, 413, 628
Rsr2I CGGWCCG 1 cut(s) 166
RsrII CGGWCCG 1 cut(s) 166
SacII CCGCGG 2 cut(s) 228, 273
SaqAI TTAA 1 cut(s) 96
SatI GCNGC 2 cut(s) 228, 497
Sau3AI GATC 4 cut(s) 75, 117, 124, 355
Sau96I GGNCC 7 cut(s) 135, 166, 171, 198, 276, 277, 459
ScaI AGTACT 1 cut(s) 414
ScrFI CCNGG 4 cut(s) 133, 134, 170, 611
SduI GDGCHC 1 cut(s) 280
SetI ASST 7 cut(s) 240, 255, 291, 444, 513, 524, 629
Sfr303I CCGCGG 2 cut(s) 228, 273
SfuI TTCGAA 1 cut(s) 346
SgrBI CCGCGG 2 cut(s) 228, 273
SinI GGWCC 1 cut(s) 166
SmaI CCCGGG 1 cut(s) 134
SrfI GCCCGGGC 1 cut(s) 134
SspMI CTAG 1 cut(s) 631
StyD4I CCNGG 4 cut(s) 131, 132, 168, 609
StyI CCWWGG 1 cut(s) 108
TaaI ACNGT 2 cut(s) 83, 539
TaiI ACGT 2 cut(s) 240, 255
TaqI TCGA 3 cut(s) 346, 390, 446
TaqII GACCGA 1 cut(s) 154
TatI WGTACW 1 cut(s) 412
TauI GCSGC 2 cut(s) 230, 499
TfiI GAWTC 3 cut(s) 60, 151, 161
Tru1I TTAA 1 cut(s) 96
Tru9I TTAA 1 cut(s) 96
TscAI CASTG 1 cut(s) 489
TseFI GTSAC 1 cut(s) 480
Tsp45I GTSAC 1 cut(s) 480
TspDTI ATGAA 1 cut(s) 376
TspGWI ACGGA 2 cut(s) 72, 408
TspMI CCCGGG 1 cut(s) 132
TspRI CASTG 1 cut(s) 489
Van91I CCANNNNNTGG 1 cut(s) 616
VpaK11BI GGWCC 1 cut(s) 166
XmaI CCCGGG 1 cut(s) 132
XspI CTAG 1 cut(s) 631
ZraI GACGTC 1 cut(s) 253
ZrmI AGTACT 1 cut(s) 414
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.