AT4G17220

Microtubule-associated protein 70

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0015178
Physical Location & Seq
Reverse (-)
9656652 .. 9659576
2925 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G17220.1

Sequence Viewer

Length: 1542 bp
ATGACTGCAGCAGAAAACCCTTTTGTATCTGACACCTCTTCTCTGCAAAGCCAGCTTAAAGAAAAAGAGAAAGAGCTTTTGGCTGCTAAAGCTGAAGTTGAGGCTTTGAGAACAAATGAAGAGCTCAAAGACAGAGTCTTTAAGGAGCTTAGAGAAAACGTGAGGAAGTTGGAGGAGAAACTTGGAGCCACCGAGAATCAAGTTGACCAAAAGGAGTTGGAGAGGAAGAAGCTAGAAGAAGAGAAGGAAGATGCATTGGCGGCACAGGACGCTGCAGAAGAAGCACTAAGGAGGGTTTACACTCACCAACAGGACGATGACTCTCTACCCCTAGAATCCATCATAGCTCCTCTTGAATCTCAGATCAAGATTCACAAGCATGAGATTTCTGCACTTCAAGAGGACAAGAAAGCGTTGGAACGTCTAACAAAATCAAAAGAATCGGCGCTTCTTGAAGCAGAGAGAATCTTGAGAAGTGCCCTTGAACGTGCTTTGATTGTTGAAGAGGTCCAGAACCATAACTTTGAACTTCGACGACAGATTGAGATATGCCAGGATGAGAACAAGTTTCTTGAGAAAATTAACCGCCAGAAAGTGTTGGAGATTGAGAAGCTCTCCCAAAGCATTGTAGAACTGGAGGAAGCGATCTTGGCAGGAGGAACAGCCGCCAATGCAGTTCGAGACTATCGCCGCCAAATCTCCCAGCTCAATGATGAGAAGAGAACCTTAGAGAGAGAGCTAGCCAGAGTCAAAGTGTCAGCAAGTAGAGTGGCACTTGCAGTTGCTAACGAGTGGAAAGATGAGAACGATAGAGTCATGCCTGTAAAGCAATGGCTTGAAGAGAGAAGGATTCTCCATGGAGAAATGCAGAAATTGAAAGATAAACTCGCTGTTTCCGAGAGAACCGCTAAGGCTGAGTCCCAGCTAAAGGAGAGGTTAAAGCTTAGGCTGAAAACCATAGAAGATGGCTTGAAGGGCCCAAACACGTTTTTCGTCTCTCCAACTACAAAGACTGAAAAATCTGGCAAGATTTTAGGATTCTTGACCAGTGGTGGTGGCTCAAAGAAAAGGTCTAGTTCTCAGCTAAGAGGCTCAGTTACAGGCAGAATCCATGCTATGAATCAGCCAATTGACAGAGTTGGGGAAAGTGATGAAATGGAGAATTCAAAGATTACTGCAAACGGATTGACTGATCAACATGAAGAAGATAGCGAAAGGAAAACAGAGGAAGATGGAAATGTTTATTCTGAAGATATGGTTTCAGGGTTTTTATATGACAGGCTTCAGAAGGAAGTGATTGCTTTAAGGAAGCTATGTGAGAGTAAAGAGGGTACTATAAATGCCAAAAATGAAGAAATCAAGATGTTGTTGAAGAAGGTGGATGCTCTAACGAAAGCCATTGAAGTTGAGACAAAGAAGGCAAAGAGGGAAGCCGCAGCGAGAGAAAAGGAAAACGCATTGGCAATGTTGAATGAAGAGTCAAAACTATGTAGAAAGGCAAAACTACCGAGAAGCCGCATACCCAATCCACGTTGCCAGTAG

Protein Analysis

513

Amino Acids

58.52

Weight (kDa)

6.34

Isoelectric Point (pI)

50.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MAP70 PF07058 6 - 368 4.1e-150 Microtubule-associated protein 70
MAP70 PF07058 388 - 498 4.1e-31 Microtubule-associated protein 70
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015178)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G17220
fragaria_vesca FvH4_3g00480 FvH4_3g00480
malus_domestica MD00G1056800.v1.1
prunus_persica Prupe.4G005400_v2.0.a1
pyrus_communis pycom05g32710
rosa_chinensis RchiOBHm_Chr5g0000651
rosa_laevigata RLG00000030885
rosa_multiflora Rmu_sc0011992.1_g000012
rosa_roxburghii Rroxscaffold_1G00075550
rosa_rugosa Rorug04G0385500
rosa_samantha Rh5AG005300 Rh5BG006900 Rh5CG005900 Rh5DG005800
rosa_wichuraiana Rw5G000550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 7 cut(s) 260, 586, 666, 691, 906, 1434, 1516
AcsI RAATTY 1 cut(s) 1162
AcuI CTGAAG 3 cut(s) 114, 1268, 1269
AfaI GTAC 1 cut(s) 1333
AfiI CCNNNNNNNGG 1 cut(s) 928
AflIII ACRYGT 1 cut(s) 984
AjnI CCWGG 1 cut(s) 552
AjuI GAANNNNNNNTTGG 2 cut(s) 62, 94
Alw21I GWGCWC 1 cut(s) 126
Alw26I GTCTC 3 cut(s) 675, 1000, 1403
AoxI GGCC 1 cut(s) 976
ApaI GGGCCC 1 cut(s) 980
ApeKI GCWGC 4 cut(s) 8, 83, 272, 1436
ApoI RAATTY 1 cut(s) 1162
ArsI GACNNNNNNTTYG 4 cut(s) 120, 152, 1055, 1087
AspLEI GCGC 1 cut(s) 448
AspS9I GGNCC 3 cut(s) 508, 976, 977
AsuHPI GGTGA 1 cut(s) 296
AsuNHI GCTAGC 1 cut(s) 739
AvaII GGWCC 1 cut(s) 508
BaeGI GKGCMC 2 cut(s) 481, 980
BanII GRGCYC 2 cut(s) 126, 980
Bbv12I GWGCWC 1 cut(s) 126
BbvI GCAGC 4 cut(s) 20, 70, 259, 1448
BccI CCATC 3 cut(s) 347, 959, 1226
BciT130I CCWGG 1 cut(s) 554
BclI TGATCA 1 cut(s) 1192
BcoDI GTCTC 3 cut(s) 675, 1000, 1403
BfaI CTAG 4 cut(s) 233, 332, 740, 1074
BfmI CTRYAG 2 cut(s) 6, 273
BfoI RGCGCY 1 cut(s) 449
BisI GCNGC 9 cut(s) 9, 84, 261, 273, 666, 691, 1434, 1437, 1516
BlsI GCNGC 9 cut(s) 10, 85, 262, 274, 667, 692, 1435, 1438, 1517
Bme1390I CCNGG 1 cut(s) 554
Bme18I GGWCC 1 cut(s) 508
BmgT120I GGNCC 3 cut(s) 508, 976, 977
BmiI GGNNCC 2 cut(s) 187, 978
BmrFI CCNGG 1 cut(s) 554
BmsI GCATC 2 cut(s) 241, 1372
BmtI GCTAGC 1 cut(s) 743
BplI GAGNNNNNCTC 2 cut(s) 599, 631
BpmI CTGGAG 1 cut(s) 656
Bpu10I CCTNAGC 2 cut(s) 909, 944
BpuEI CTTGAG 2 cut(s) 490, 593
BsaJI CCNNGG 1 cut(s) 856
Bsc4I CCNNNNNNNGG 1 cut(s) 928
Bse1I ACTGG 3 cut(s) 639, 1047, 1537
Bse3DI GCAATG 2 cut(s) 836, 1470
BseBI CCWGG 1 cut(s) 554
BseDI CCNNGG 1 cut(s) 856
BseGI GGATG 2 cut(s) 562, 1387
BseLI CCNNNNNNNGG 1 cut(s) 928
BseMI GCAATG 2 cut(s) 836, 1470
BseMII CTCAG 4 cut(s) 374, 906, 1094, 1107
BseNI ACTGG 3 cut(s) 639, 1047, 1537
BseRI GAGGAG 2 cut(s) 188, 339
BseSI GKGCMC 2 cut(s) 481, 980
BseXI GCAGC 4 cut(s) 20, 70, 259, 1448
BseYI CCCAGC 2 cut(s) 702, 921
BsgI GTGCAG 1 cut(s) 375
BshFI GGCC 1 cut(s) 978
BsiHKAI GWGCWC 1 cut(s) 126
BslFI GGGAC 1 cut(s) 904
BslI CCNNNNNNNGG 1 cut(s) 928
BsmAI GTCTC 3 cut(s) 675, 1000, 1403
BsmBI CGTCTC 1 cut(s) 1000
BsmFI GGGAC 1 cut(s) 904
BsnI GGCC 1 cut(s) 978
Bsp120I GGGCCC 1 cut(s) 976
Bsp1286I GDGCHC 3 cut(s) 126, 481, 980
Bsp143I GATC 3 cut(s) 363, 645, 1192
Bsp19I CCATGG 1 cut(s) 856
BspACI CCGC 7 cut(s) 260, 586, 666, 691, 906, 1434, 1516
BspANI GGCC 1 cut(s) 978
BspCNI CTCAG 4 cut(s) 373, 907, 1093, 1106
BspLI GGNNCC 2 cut(s) 187, 978
BspMAI CTGCAG 2 cut(s) 10, 277
BspOI GCTAGC 1 cut(s) 743
BspQI GCTCTTC 1 cut(s) 114
BsrDI GCAATG 2 cut(s) 836, 1470
BsrI ACTGG 3 cut(s) 639, 1047, 1537
BssECI CCNNGG 1 cut(s) 856
BssMI GATC 3 cut(s) 363, 645, 1192
BssT1I CCWWGG 1 cut(s) 856
Bst2UI CCWGG 1 cut(s) 554
Bst6I CTCTTC 7 cut(s) 43, 114, 234, 498, 713, 834, 1470
BstC8I GCNNGC 2 cut(s) 53, 741
BstDSI CCRYGG 1 cut(s) 856
BstF5I GGATG 2 cut(s) 562, 1387
BstH2I RGCGCY 1 cut(s) 449
BstHHI GCGC 1 cut(s) 448
BstKTI GATC 3 cut(s) 366, 648, 1195
BstMAI GTCTC 3 cut(s) 675, 1000, 1403
BstMBI GATC 3 cut(s) 363, 645, 1192
BstMWI GCNNNNNNNGC 9 cut(s) 52, 89, 260, 269, 281, 650, 671, 826, 975
BstNI CCWGG 1 cut(s) 554
BstSCI CCNGG 1 cut(s) 552
BstSFI CTRYAG 2 cut(s) 6, 273
BstSLI GKGCMC 2 cut(s) 481, 980
BstV1I GCAGC 4 cut(s) 20, 70, 259, 1448
BsuRI GGCC 1 cut(s) 978
BtgI CCRYGG 1 cut(s) 856
BtsCI GGATG 2 cut(s) 562, 1387
BtsIMutI CAGTG 1 cut(s) 1054
Cac8I GCNNGC 2 cut(s) 53, 741
CfoI GCGC 1 cut(s) 448
Cfr13I GGNCC 3 cut(s) 508, 976, 977
CseI GACGC 1 cut(s) 278
Csp6I GTAC 1 cut(s) 1332
CspCI CAANNNNNGTGG 2 cut(s) 750, 785
CviAII CATG 5 cut(s) 380, 817, 857, 1112, 1199
CviQI GTAC 1 cut(s) 1332
DpnI GATC 3 cut(s) 365, 647, 1194
DpnII GATC 3 cut(s) 363, 645, 1192
Eam1104I CTCTTC 7 cut(s) 43, 114, 234, 498, 713, 834, 1470
EarI CTCTTC 7 cut(s) 43, 114, 234, 498, 713, 834, 1470
Ecl136II GAGCTC 1 cut(s) 124
Eco130I CCWWGG 1 cut(s) 856
Eco24I GRGCYC 2 cut(s) 126, 980
Eco47I GGWCC 1 cut(s) 508
Eco53kI GAGCTC 1 cut(s) 124
Eco57I CTGAAG 3 cut(s) 114, 1268, 1269
EcoICRI GAGCTC 1 cut(s) 124
EcoO109I RGGNCCY 1 cut(s) 976
EcoRI GAATTC 1 cut(s) 1162
EcoRII CCWGG 1 cut(s) 552
EcoT14I CCWWGG 1 cut(s) 856
EcoT22I ATGCAT 1 cut(s) 256
EcoT38I GRGCYC 2 cut(s) 126, 980
ErhI CCWWGG 1 cut(s) 856
Esp3I CGTCTC 1 cut(s) 1000
FaeI CATG 5 cut(s) 383, 820, 860, 1115, 1202
FalI AAGNNNNNCTT 2 cut(s) 710, 742
FaqI GGGAC 1 cut(s) 904
FatI CATG 5 cut(s) 379, 816, 856, 1111, 1198
FbaI TGATCA 1 cut(s) 1192
Fnu4HI GCNGC 9 cut(s) 9, 84, 261, 273, 666, 691, 1434, 1437, 1516
FokI GGATG 2 cut(s) 569, 1394
FriOI GRGCYC 2 cut(s) 126, 980
Fsp4HI GCNGC 9 cut(s) 9, 84, 261, 273, 666, 691, 1434, 1437, 1516
FspBI CTAG 4 cut(s) 233, 332, 740, 1074
GlaI GCGC 1 cut(s) 447
GluI GCNGC 9 cut(s) 9, 84, 261, 273, 666, 691, 1434, 1437, 1516
GsaI CCCAGC 2 cut(s) 706, 925
GsuI CTGGAG 1 cut(s) 656
HaeII RGCGCY 1 cut(s) 449
HaeIII GGCC 1 cut(s) 978
HgaI GACGC 1 cut(s) 278
HhaI GCGC 1 cut(s) 448
Hin1II CATG 5 cut(s) 383, 820, 860, 1115, 1202
Hin6I GCGC 1 cut(s) 446
HinP1I GCGC 1 cut(s) 446
HincII GTYRAC 1 cut(s) 205
HindII GTYRAC 1 cut(s) 205
HindIII AAGCTT 1 cut(s) 941
HphI GGTGA 1 cut(s) 296
Hpy166II GTNNAC 2 cut(s) 205, 298
Hpy188I TCNGA 5 cut(s) 31, 363, 898, 1249, 1287
Hpy8I GTNNAC 2 cut(s) 205, 298
Hpy99I CGWCG 1 cut(s) 537
HpyAV CCTTC 6 cut(s) 238, 840, 967, 1282, 1369, 1411
HpyCH4IV ACGT 5 cut(s) 159, 421, 487, 986, 1531
HpyCH4V TGCA 9 cut(s) 8, 46, 254, 275, 392, 674, 779, 868, 1178
HpyF10VI GCNNNNNNNGC 9 cut(s) 52, 89, 260, 269, 281, 650, 671, 826, 975
HpySE526I ACGT 5 cut(s) 159, 421, 487, 986, 1531
Hsp92II CATG 5 cut(s) 383, 820, 860, 1115, 1202
HspAI GCGC 1 cut(s) 446
Ksp22I TGATCA 1 cut(s) 1192
Kzo9I GATC 3 cut(s) 363, 645, 1192
LguI GCTCTTC 1 cut(s) 114
LmnI GCTCC 3 cut(s) 145, 185, 352
Lsp1109I GCAGC 4 cut(s) 20, 70, 259, 1448
LweI GCATC 2 cut(s) 241, 1372
MaeI CTAG 4 cut(s) 233, 332, 740, 1074
MaeII ACGT 5 cut(s) 159, 421, 487, 986, 1531
MaeIII GTNAC 1 cut(s) 1096
MalI GATC 3 cut(s) 365, 647, 1194
MboI GATC 3 cut(s) 363, 645, 1192
MfeI CAATTG 1 cut(s) 1128
MhlI GDGCHC 3 cut(s) 126, 481, 980
MluCI AATT 4 cut(s) 579, 872, 1128, 1162
MlyI GAGTC 6 cut(s) 144, 314, 756, 822, 926, 1487
MmeI TCCRAC 5 cut(s) 150, 198, 396, 579, 1025
Mph1103I ATGCAT 1 cut(s) 256
MseI TTAA 5 cut(s) 57, 141, 582, 938, 1304
MslI CAYNNNNRTG 1 cut(s) 378
MspR9I CCNGG 1 cut(s) 554
MunI CAATTG 1 cut(s) 1128
MvaI CCWGG 1 cut(s) 554
MwoI GCNNNNNNNGC 9 cut(s) 52, 89, 260, 269, 281, 650, 671, 826, 975
NcoI CCATGG 1 cut(s) 856
NdeII GATC 3 cut(s) 363, 645, 1192
NheI GCTAGC 1 cut(s) 739
NlaIII CATG 5 cut(s) 383, 820, 860, 1115, 1202
NlaIV GGNNCC 2 cut(s) 187, 978
NsiI ATGCAT 1 cut(s) 256
PciSI GCTCTTC 1 cut(s) 114
PcsI WCGNNNNNNNCGW 1 cut(s) 894
PflFI GACNNNGTC 1 cut(s) 134
PkrI GCNGC 9 cut(s) 10, 85, 262, 274, 667, 692, 1435, 1438, 1517
PleI GAGTC 6 cut(s) 143, 314, 755, 821, 925, 1486
PpsI GAGTC 6 cut(s) 143, 314, 755, 821, 925, 1486
Psp124BI GAGCTC 1 cut(s) 126
Psp6I CCWGG 1 cut(s) 552
PspFI CCCAGC 2 cut(s) 702, 921
PspGI CCWGG 1 cut(s) 552
PspN4I GGNNCC 2 cut(s) 187, 978
PspOMI GGGCCC 1 cut(s) 976
PspPI GGNCC 3 cut(s) 508, 976, 977
PstI CTGCAG 2 cut(s) 10, 277
PsyI GACNNNGTC 1 cut(s) 134
RsaI GTAC 1 cut(s) 1333
RsaNI GTAC 1 cut(s) 1332
RseI CAYNNNNRTG 1 cut(s) 378
SacI GAGCTC 1 cut(s) 126
SapI GCTCTTC 1 cut(s) 114
SaqAI TTAA 5 cut(s) 57, 141, 582, 938, 1304
SatI GCNGC 9 cut(s) 9, 84, 261, 273, 666, 691, 1434, 1437, 1516
Sau3AI GATC 3 cut(s) 363, 645, 1192
Sau96I GGNCC 3 cut(s) 508, 976, 977
SchI GAGTC 6 cut(s) 144, 314, 756, 822, 926, 1487
ScrFI CCNGG 1 cut(s) 554
SduI GDGCHC 3 cut(s) 126, 481, 980
SfaNI GCATC 2 cut(s) 241, 1372
SfcI CTRYAG 2 cut(s) 6, 273
SinI GGWCC 1 cut(s) 508
SmiMI CAYNNNNRTG 1 cut(s) 378
SmlI CTYRAG 2 cut(s) 469, 572
SmoI CTYRAG 2 cut(s) 469, 572
Sse9I AATT 4 cut(s) 579, 872, 1128, 1162
SsiI CCGC 7 cut(s) 260, 586, 666, 691, 906, 1434, 1516
SspMI CTAG 4 cut(s) 233, 332, 740, 1074
SstI GAGCTC 1 cut(s) 126
StyD4I CCNGG 1 cut(s) 552
StyI CCWWGG 1 cut(s) 856
TaiI ACGT 5 cut(s) 162, 424, 490, 989, 1534
TaqI TCGA 2 cut(s) 532, 679
TasI AATT 4 cut(s) 579, 872, 1128, 1162
TauI GCSGC 5 cut(s) 263, 668, 693, 1436, 1518
Tru1I TTAA 5 cut(s) 57, 141, 582, 938, 1304
Tru9I TTAA 5 cut(s) 57, 141, 582, 938, 1304
TscAI CASTG 1 cut(s) 1054
TseI GCWGC 4 cut(s) 8, 83, 272, 1436
TspDTI ATGAA 6 cut(s) 132, 1133, 1167, 1215, 1365, 1488
TspGWI ACGGA 1 cut(s) 1197
TspRI CASTG 1 cut(s) 1054
Tth111I GACNNNGTC 1 cut(s) 134
VpaK11BI GGWCC 1 cut(s) 508
XapI RAATTY 1 cut(s) 1162
XspI CTAG 4 cut(s) 233, 332, 740, 1074
Zsp2I ATGCAT 1 cut(s) 256
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.