RLG00000030885

Microtubule-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0015178
Physical Location & Seq
Reverse (-)
432389 .. 435723
3335 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030885

Sequence Viewer

Length: 1662 bp
ATGATAAGAAACTTGTGCTTGTCAAATTTTGCTTCACAGCAAAATAAGGGAGATATGGTGGCGGGTCATGAACACCACCTACAACATGCAGCAGGAGGAGGAGGAGGAGAGGACCTCTCTCTTGTCTCCGCAGACCCTGTTGTCTTAGAGCTCAATCGTATGCAGAACCTACTCAAAGAAAAGAGCCGCGAGTTGGGGATTGCTCAGGGTGAGATCAAGGCTCTCAGAGCTGCTGAAGTTCTAAAGGATAAAGCTGTAGAAGAGCTAAGTAATGAAGTAGAGAAGCTGGATGAGAAACATAGGCTCACGCAAAATCTTCTAGAGCATAAGAACCTTGAAATCAAGAAACTAGCAGATGAGAAAAGAGACGCATTAGCCGCACAATATGCTGCAGAAGCTACTCTTAGAAGGGTACACATAAATCAAAAGGATGATGAACTTCCTTCTCTTGAATCTGTCATTGCTCCTCTCGAGGCTGAAATCAAAATGTGCAAGAATGAGATTGCAGCTCTTCAGGACGACAACAAGGCTTTGGAACGTCTCACCAAGTCGAAAGAGTCCGCTCTTCTTGAGGCAGAAAGGATATTGCGAAGTGCACTAGAAAGAGCTCTAATAGTTGAAGAGGTCCAAAACCAGAACTTCGAACTGAGGAGACAAATTGAAATCTGCCAGGAAGAAAACAGAATCCTTGAGAAAACAAATCGCCAGAAGGTACTAGAGGTTGAAAAGCTTAGCCAAACCATTCAAGAACTTGAGGAGGCCATTCTAGCTGGAGGGGCTGCTGCCAATGCTGTTCGTGACTACAAAAGACAGATAGATGAATTGCATGAGGAGAAGAGGACATTGGAGAGGGAGCTAGCAAGAGTGAAAGTTTCTGCAAACCGGGTAGCTACTGTTATTGCTAACGAGTGGAAGGATGAAAACGACAAGGTTATGCCTGTTAAACAGTGGCTGGAAGAGAGAAGACTAATGCAGGCAGAGATGCAACGTATGAGGGAGAAGCTAGCAATCTCGGATAGAACAGCCAAGGCAGAGGCACAACTCAAGGACAAGCTGAAGTTGAGGCTGAAGACTCTAGAAGAAGGGTTAAAGCATGTTTCAAGTTTCTCTGTCAATCCTAATTCATTTTGCCGTTCCCCTAAAACAGAGAAGTCCAGTAACTTCTTAGGATTTCTGACAAGCCCTGGTGGATTAGGAAAGAGATCGACTTCACAGCCAAGAGCCTCCACTCTTAGAAGCTCTCCTCTCCAACAGCCTAATTCAGACAATGAAACAGCCAATGCTGCCGGAGAGCTAAAAAGATCAAATAGCTTTAAAAAGAGATATGGTTCAGGAGAAAAAATGCTGAGAAAAAGCTTATGGGCTTCTAGAACTAAAGTTGTTGATAGTGGTGAGAAGGAGAACACAGAACAGAAAGCAAATACTGATAACAACAAGAATGATGATGAGACAGTTACTGCTGAGATAAAAACTAAAGATGGTGCCAATGAAGACTCACCCAACAGGATAAGCATTAACTGTGAAAGTGAAGATGTGGTCTCAGGATTTCTCTATGACAGACTTCAAAAGGAGGTCATCAATCTGAGGAGGCATTGTGAAGTCAAAGAGAGTGATATGAATGCGAAAGAACAAGAAATAAAGAAAGAAAAGTTTCACCAGTGA

Protein Analysis

554

Amino Acids

62.68

Weight (kDa)

6.5

Isoelectric Point (pI)

49.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MAP70 PF07058 41 - 547 3e-203 Microtubule-associated protein 70
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015178)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G17220
fragaria_vesca FvH4_3g00480 FvH4_3g00480
malus_domestica MD00G1056800.v1.1
prunus_persica Prupe.4G005400_v2.0.a1
pyrus_communis pycom05g32710
rosa_chinensis RchiOBHm_Chr5g0000651
rosa_laevigata RLG00000030885
rosa_multiflora Rmu_sc0011992.1_g000012
rosa_roxburghii Rroxscaffold_1G00075550
rosa_rugosa Rorug04G0385500
rosa_samantha Rh5AG005300 Rh5BG006900 Rh5CG005900 Rh5DG005800
rosa_wichuraiana Rw5G000550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 140
AccB1I GGYRCC 1 cut(s) 1481
AccBSI CCGCTC 1 cut(s) 563
AccII CGCG 1 cut(s) 189
AciI CCGC 5 cut(s) 62, 129, 187, 378, 561
AcsI RAATTY 1 cut(s) 25
AcuI CTGAAG 4 cut(s) 255, 497, 1076, 1088
AfaI GTAC 2 cut(s) 414, 714
AfiI CCNNNNNNNGG 1 cut(s) 193
AgsI TTSAA 8 cut(s) 338, 452, 620, 662, 725, 746, 1101, 1565
AjnI CCWGG 2 cut(s) 669, 1183
AjuI GAANNNNNNNTTGG 2 cut(s) 827, 859
Alw21I GWGCWC 3 cut(s) 153, 598, 610
Alw26I GTCTC 6 cut(s) 130, 360, 545, 646, 1442, 1543
Alw44I GTGCAC 1 cut(s) 594
AlwNI CAGNNNCTG 3 cut(s) 137, 952, 1457
Ama87I CYCGRG 1 cut(s) 470
AoxI GGCC 1 cut(s) 759
ApaLI GTGCAC 1 cut(s) 594
ApeKI GCWGC 7 cut(s) 89, 230, 389, 506, 779, 782, 1283
ApoI RAATTY 1 cut(s) 25
Asp700I GAANNNNTTC 1 cut(s) 1650
AspS9I GGNCC 2 cut(s) 112, 625
AsuC2I CCSGG 1 cut(s) 884
AsuHPI GGTGA 5 cut(s) 221, 535, 1403, 1488, 1646
AsuII TTCGAA 1 cut(s) 642
AsuNHI GCTAGC 2 cut(s) 856, 1003
AvaI CYCGRG 1 cut(s) 470
AvaII GGWCC 2 cut(s) 112, 625
BaeGI GKGCMC 1 cut(s) 598
BanI GGYRCC 1 cut(s) 1481
BanII GRGCYC 2 cut(s) 153, 610
BbsI GAAGAC 3 cut(s) 970, 1076, 1497
Bbv12I GWGCWC 3 cut(s) 153, 598, 610
BbvI GCAGC 7 cut(s) 101, 217, 376, 518, 766, 769, 1270
BccI CCATC 1 cut(s) 1472
BceAI ACGGC 1 cut(s) 1116
BciT130I CCWGG 2 cut(s) 671, 1185
BcnI CCSGG 1 cut(s) 884
BcoDI GTCTC 6 cut(s) 130, 360, 545, 646, 1442, 1543
BfaI CTAG 9 cut(s) 320, 350, 599, 716, 767, 857, 1004, 1076, 1368
BfmI CTRYAG 2 cut(s) 255, 390
BisI GCNGC 9 cut(s) 90, 187, 231, 378, 390, 507, 780, 783, 1284
BlpI GCTNAGC 1 cut(s) 731
BlsI GCNGC 9 cut(s) 91, 188, 232, 379, 391, 508, 781, 784, 1285
Bme1390I CCNGG 3 cut(s) 671, 884, 1185
Bme18I GGWCC 2 cut(s) 112, 625
BmeT110I CYCGRG 1 cut(s) 470
BmgT120I GGNCC 2 cut(s) 112, 625
BmiI GGNNCC 1 cut(s) 1483
BmrFI CCNGG 3 cut(s) 671, 884, 1185
BmsI GCATC 1 cut(s) 972
BmtI GCTAGC 2 cut(s) 860, 1007
BpiI GAAGAC 3 cut(s) 970, 1076, 1497
BplI GAGNNNNNCTC 4 cut(s) 99, 131, 101, 133
BpmI CTGGAG 1 cut(s) 792
Bpu10I CCTNAGC 1 cut(s) 204
Bpu1102I GCTNAGC 1 cut(s) 731
Bpu14I TTCGAA 1 cut(s) 642
BpuEI CTTGAG 4 cut(s) 590, 710, 773, 1028
BpuMI CCSGG 1 cut(s) 884
BsaI GGTCTC 1 cut(s) 1543
BsaJI CCNNGG 2 cut(s) 1026, 1183
BsaXI ACNNNNNCTCC 4 cut(s) 42, 72, 1579, 1609
Bsc4I CCNNNNNNNGG 1 cut(s) 193
Bse1I ACTGG 2 cut(s) 1155, 1657
Bse3DI GCAATG 1 cut(s) 459
BseBI CCWGG 2 cut(s) 671, 1185
BseDI CCNNGG 2 cut(s) 1026, 1183
BseGI GGATG 3 cut(s) 295, 436, 922
BseLI CCNNNNNNNGG 1 cut(s) 193
BseMI GCAATG 1 cut(s) 459
BseMII CTCAG 7 cut(s) 218, 238, 638, 1337, 1452, 1554, 1574
BseNI ACTGG 2 cut(s) 1155, 1657
BseSI GKGCMC 1 cut(s) 598
BseXI GCAGC 7 cut(s) 101, 217, 376, 518, 766, 769, 1270
Bsh1236I CGCG 1 cut(s) 189
BshFI GGCC 1 cut(s) 761
BshNI GGYRCC 1 cut(s) 1481
BsiHKAI GWGCWC 3 cut(s) 153, 598, 610
BsiHKCI CYCGRG 1 cut(s) 470
BsiSI CCGG 2 cut(s) 883, 1287
BslI CCNNNNNNNGG 1 cut(s) 193
BsmAI GTCTC 6 cut(s) 130, 360, 545, 646, 1442, 1543
BsmBI CGTCTC 2 cut(s) 360, 545
BsmI GAATGC 1 cut(s) 1624
BsnI GGCC 1 cut(s) 761
Bso31I GGTCTC 1 cut(s) 1543
BsoBI CYCGRG 1 cut(s) 470
Bsp119I TTCGAA 1 cut(s) 642
Bsp1286I GDGCHC 3 cut(s) 153, 598, 610
Bsp143I GATC 3 cut(s) 213, 1202, 1301
Bsp1720I GCTNAGC 1 cut(s) 731
BspACI CCGC 5 cut(s) 62, 129, 187, 378, 561
BspANI GGCC 1 cut(s) 761
BspCNI CTCAG 7 cut(s) 217, 237, 639, 1338, 1453, 1553, 1575
BspFNI CGCG 1 cut(s) 189
BspHI TCATGA 1 cut(s) 67
BspLI GGNNCC 1 cut(s) 1483
BspMAI CTGCAG 1 cut(s) 394
BspOI GCTAGC 2 cut(s) 860, 1007
BspQI GCTCTTC 3 cut(s) 255, 516, 570
BspT104I TTCGAA 1 cut(s) 642
BspT107I GGYRCC 1 cut(s) 1481
BspTNI GGTCTC 1 cut(s) 1543
BsrBI CCGCTC 1 cut(s) 563
BsrDI GCAATG 1 cut(s) 459
BsrI ACTGG 2 cut(s) 1155, 1657
BssECI CCNNGG 2 cut(s) 1026, 1183
BssMI GATC 3 cut(s) 213, 1202, 1301
BssT1I CCWWGG 1 cut(s) 1026
Bst2UI CCWGG 2 cut(s) 671, 1185
Bst4CI ACNGT 4 cut(s) 895, 948, 1453, 1520
Bst6I CTCTTC 6 cut(s) 255, 516, 570, 615, 830, 951
BstAPI GCANNNNNTGC 1 cut(s) 386
BstBI TTCGAA 1 cut(s) 642
BstC8I GCNNGC 3 cut(s) 858, 975, 1005
BstF5I GGATG 3 cut(s) 295, 436, 922
BstFNI CGCG 1 cut(s) 189
BstKTI GATC 3 cut(s) 216, 1205, 1304
BstMAI GTCTC 6 cut(s) 130, 360, 545, 646, 1442, 1543
BstMBI GATC 3 cut(s) 213, 1202, 1301
BstMWI GCNNNNNNNGC 8 cut(s) 227, 377, 386, 395, 767, 776, 788, 1283
BstNI CCWGG 2 cut(s) 671, 1185
BstNSI RCATGY 2 cut(s) 89, 1097
BstSCI CCNGG 3 cut(s) 669, 882, 1183
BstSFI CTRYAG 2 cut(s) 255, 390
BstSLI GKGCMC 1 cut(s) 598
BstUI CGCG 1 cut(s) 189
BstV1I GCAGC 7 cut(s) 101, 217, 376, 518, 766, 769, 1270
BstV2I GAAGAC 3 cut(s) 970, 1076, 1497
BsuRI GGCC 1 cut(s) 761
BtsCI GGATG 3 cut(s) 295, 436, 922
BtsIMutI CAGTG 1 cut(s) 953
Cac8I GCNNGC 3 cut(s) 858, 975, 1005
CaiI CAGNNNCTG 3 cut(s) 137, 952, 1457
CciI TCATGA 1 cut(s) 67
Cfr13I GGNCC 2 cut(s) 112, 625
CseI GACGC 1 cut(s) 377
Csp6I GTAC 2 cut(s) 413, 713
CviAII CATG 4 cut(s) 68, 86, 827, 1094
CviQI GTAC 2 cut(s) 413, 713
DpnI GATC 3 cut(s) 215, 1204, 1303
DpnII GATC 3 cut(s) 213, 1202, 1301
DraI TTTAAA 1 cut(s) 1315
DrdI GACNNNNNNGTC 1 cut(s) 140
DseDI GACNNNNNNGTC 1 cut(s) 140
Eam1104I CTCTTC 6 cut(s) 255, 516, 570, 615, 830, 951
EarI CTCTTC 6 cut(s) 255, 516, 570, 615, 830, 951
Ecl136II GAGCTC 2 cut(s) 151, 608
Eco130I CCWWGG 1 cut(s) 1026
Eco24I GRGCYC 2 cut(s) 153, 610
Eco31I GGTCTC 1 cut(s) 1543
Eco47I GGWCC 2 cut(s) 112, 625
Eco53kI GAGCTC 2 cut(s) 151, 608
Eco57I CTGAAG 4 cut(s) 255, 497, 1076, 1088
Eco88I CYCGRG 1 cut(s) 470
EcoICRI GAGCTC 2 cut(s) 151, 608
EcoO109I RGGNCCY 1 cut(s) 112
EcoRII CCWGG 2 cut(s) 669, 1183
EcoT14I CCWWGG 1 cut(s) 1026
EcoT38I GRGCYC 2 cut(s) 153, 610
ErhI CCWWGG 1 cut(s) 1026
Esp3I CGTCTC 2 cut(s) 360, 545
FaeI CATG 4 cut(s) 71, 89, 830, 1097
FalI AAGNNNNNCTT 2 cut(s) 387, 419
FatI CATG 4 cut(s) 67, 85, 826, 1093
FauI CCCGC 1 cut(s) 55
Fnu4HI GCNGC 9 cut(s) 90, 187, 231, 378, 390, 507, 780, 783, 1284
FokI GGATG 3 cut(s) 302, 443, 929
FriOI GRGCYC 2 cut(s) 153, 610
Fsp4HI GCNGC 9 cut(s) 90, 187, 231, 378, 390, 507, 780, 783, 1284
FspBI CTAG 9 cut(s) 320, 350, 599, 716, 767, 857, 1004, 1076, 1368
GluI GCNGC 9 cut(s) 90, 187, 231, 378, 390, 507, 780, 783, 1284
GsuI CTGGAG 1 cut(s) 792
HaeIII GGCC 1 cut(s) 761
HapII CCGG 2 cut(s) 883, 1287
HgaI GACGC 1 cut(s) 377
Hin1II CATG 4 cut(s) 71, 89, 830, 1097
HindIII AAGCTT 2 cut(s) 728, 1354
HinfI GANTC 5 cut(s) 452, 557, 684, 1072, 1493
HpaII CCGG 2 cut(s) 883, 1287
HphI GGTGA 5 cut(s) 221, 535, 1403, 1488, 1646
Hpy166II GTNNAC 2 cut(s) 415, 596
Hpy188I TCNGA 5 cut(s) 227, 1015, 1176, 1264, 1584
Hpy8I GTNNAC 2 cut(s) 415, 596
HpyAV CCTTC 6 cut(s) 402, 453, 703, 907, 1076, 1390
HpyCH4III ACNGT 4 cut(s) 895, 948, 1453, 1520
HpyCH4IV ACGT 2 cut(s) 538, 988
HpyF10VI GCNNNNNNNGC 8 cut(s) 227, 377, 386, 395, 767, 776, 788, 1283
HpySE526I ACGT 2 cut(s) 538, 988
Hsp92II CATG 4 cut(s) 71, 89, 830, 1097
Kzo9I GATC 3 cut(s) 213, 1202, 1301
LguI GCTCTTC 3 cut(s) 255, 516, 570
LmnI GCTCC 2 cut(s) 469, 853
Lsp1109I GCAGC 7 cut(s) 101, 217, 376, 518, 766, 769, 1270
LweI GCATC 1 cut(s) 972
MaeI CTAG 9 cut(s) 320, 350, 599, 716, 767, 857, 1004, 1076, 1368
MaeII ACGT 2 cut(s) 538, 988
MaeIII GTNAC 3 cut(s) 797, 1157, 1453
MalI GATC 3 cut(s) 215, 1204, 1303
MbiI CCGCTC 1 cut(s) 563
MboI GATC 3 cut(s) 213, 1202, 1301
MhlI GDGCHC 3 cut(s) 153, 598, 610
MluCI AATT 5 cut(s) 25, 657, 821, 1120, 1258
MlyI GAGTC 3 cut(s) 566, 1066, 1487
MmeI TCCRAC 1 cut(s) 1273
MroXI GAANNNNTTC 1 cut(s) 1650
MseI TTAA 4 cut(s) 942, 1088, 1314, 1515
MspI CCGG 2 cut(s) 883, 1287
MspR9I CCNGG 3 cut(s) 671, 884, 1185
Mva1269I GAATGC 1 cut(s) 1624
MvaI CCWGG 2 cut(s) 671, 1185
MvnI CGCG 1 cut(s) 189
MwoI GCNNNNNNNGC 8 cut(s) 227, 377, 386, 395, 767, 776, 788, 1283
NciI CCSGG 1 cut(s) 884
NdeII GATC 3 cut(s) 213, 1202, 1301
NheI GCTAGC 2 cut(s) 856, 1003
NlaIII CATG 4 cut(s) 71, 89, 830, 1097
NlaIV GGNNCC 1 cut(s) 1483
NmuCI GTSAC 1 cut(s) 797
NspI RCATGY 2 cut(s) 89, 1097
NspV TTCGAA 1 cut(s) 642
PaeR7I CTCGAG 1 cut(s) 470
PagI TCATGA 1 cut(s) 67
PciSI GCTCTTC 3 cut(s) 255, 516, 570
PctI GAATGC 1 cut(s) 1624
PdmI GAANNNNTTC 1 cut(s) 1650
PfeI GAWTC 2 cut(s) 452, 684
PkrI GCNGC 9 cut(s) 91, 188, 232, 379, 391, 508, 781, 784, 1285
PleI GAGTC 3 cut(s) 565, 1066, 1487
PpsI GAGTC 3 cut(s) 565, 1066, 1487
PpuMI RGGWCCY 1 cut(s) 112
Psp124BI GAGCTC 2 cut(s) 153, 610
Psp5II RGGWCCY 1 cut(s) 112
Psp6I CCWGG 2 cut(s) 669, 1183
PspGI CCWGG 2 cut(s) 669, 1183
PspN4I GGNNCC 1 cut(s) 1483
PspPI GGNCC 2 cut(s) 112, 625
PspPPI RGGWCCY 1 cut(s) 112
PsrI GAACNNNNNNTAC 2 cut(s) 63, 95
PstI CTGCAG 1 cut(s) 394
PstNI CAGNNNCTG 3 cut(s) 137, 952, 1457
RsaI GTAC 2 cut(s) 414, 714
RsaNI GTAC 2 cut(s) 413, 713
SacI GAGCTC 2 cut(s) 153, 610
SapI GCTCTTC 3 cut(s) 255, 516, 570
SaqAI TTAA 4 cut(s) 942, 1088, 1314, 1515
SatI GCNGC 9 cut(s) 90, 187, 231, 378, 390, 507, 780, 783, 1284
Sau3AI GATC 3 cut(s) 213, 1202, 1301
Sau96I GGNCC 2 cut(s) 112, 625
SchI GAGTC 3 cut(s) 566, 1066, 1487
ScrFI CCNGG 3 cut(s) 671, 884, 1185
SduI GDGCHC 3 cut(s) 153, 598, 610
SfaNI GCATC 1 cut(s) 972
SfcI CTRYAG 2 cut(s) 255, 390
Sfr274I CTCGAG 1 cut(s) 470
SfuI TTCGAA 1 cut(s) 642
SinI GGWCC 2 cut(s) 112, 625
SlaI CTCGAG 1 cut(s) 470
SmlI CTYRAG 5 cut(s) 470, 569, 689, 752, 1043
SmoI CTYRAG 5 cut(s) 470, 569, 689, 752, 1043
Sse9I AATT 5 cut(s) 25, 657, 821, 1120, 1258
SsiI CCGC 5 cut(s) 62, 129, 187, 378, 561
SspMI CTAG 9 cut(s) 320, 350, 599, 716, 767, 857, 1004, 1076, 1368
SstI GAGCTC 2 cut(s) 153, 610
StyD4I CCNGG 3 cut(s) 669, 882, 1183
StyI CCWWGG 1 cut(s) 1026
TaaI ACNGT 4 cut(s) 895, 948, 1453, 1520
TaiI ACGT 2 cut(s) 541, 991
TaqI TCGA 4 cut(s) 471, 551, 642, 1205
TasI AATT 5 cut(s) 25, 657, 821, 1120, 1258
TauI GCSGC 2 cut(s) 189, 380
TfiI GAWTC 2 cut(s) 452, 684
Tru1I TTAA 4 cut(s) 942, 1088, 1314, 1515
Tru9I TTAA 4 cut(s) 942, 1088, 1314, 1515
TscAI CASTG 1 cut(s) 953
TseFI GTSAC 1 cut(s) 797
TseI GCWGC 7 cut(s) 89, 230, 389, 506, 779, 782, 1283
Tsp45I GTSAC 1 cut(s) 797
TspDTI ATGAA 9 cut(s) 84, 288, 450, 834, 933, 1113, 1284, 1503, 1631
TspRI CASTG 1 cut(s) 953
VneI GTGCAC 1 cut(s) 594
VpaK11BI GGWCC 2 cut(s) 112, 625
XapI RAATTY 1 cut(s) 25
XbaI TCTAGA 3 cut(s) 319, 1075, 1367
XceI RCATGY 2 cut(s) 89, 1097
XhoI CTCGAG 1 cut(s) 470
XmnI GAANNNNTTC 1 cut(s) 1650
XspI CTAG 9 cut(s) 320, 350, 599, 716, 767, 857, 1004, 1076, 1368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.