FvH4_3g00480

Microtubule-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0015178
Physical Location & Seq
Reverse (-)
255418 .. 260110
4693 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g00480.t2

Sequence Viewer

Length: 1749 bp
ATGGTGGCGGGTCAGGAACAACACCTACAACTTGCAGGAGGAGGAGCAGAGGACCAATCTCTTGTCACTGCAGACCCTGTCGTCTTAGAACTCAATCGCATGCACAACGTACTCAAAGAAAAGAATCGTGAGTTGGGGATTGCTCAAGGTGAGATCAAGGCTCTCAGAGCTACTGAAGTTCTTAAGGATAAAGCTGCAGAAGAGCTAAGAAATGAAGTAGAGAAGCTGGATGAGAAACACAGACTCACGCAAACTCTTCTAGAACAAAAGAACCTTGAAATCAAGAAACTGACAGACGAGAAAAAAGATGCATTGGCTGCACAATATGCTGCAGAAGCAACTCTTAGAAGGGTACACACAAGTCGAAAGGATGATGATCTTCCTTCTCTTGAATCTATCATTGCTCCACTCGAGGCTGAAATCAAAATGTGCAAGAATGAGATTGCAGCACTTCAGGAGGACAAGAAGGCTTTGGAACGTCTTACCAAGTCGAAAGAATTGGCTCTTCTTGAGGCAGAAAGGATATTACGAAGTGCACTTGAAAGAGCTTTAATAGTTGAAGAGGTTCAAAACCAAAACTTTGAATTAAGGAGACAGATTGAAATCTGCCAGGAGGAAAACAGAATCCTTGAGAGAACAAATCGCCAAAAGGTTTTAGAGGTTGAAAAGCTTAGCCAGACTATTCAAGAACTTGAGGAGGCCATTTTAGCTGGAGGGGCTGCTGCCAATGCTATTCGTGACTACAAAAGACAAATAGATGAATTGCATGATGAGAAGAAGACTCTGGAGAGGGAGCTAGCAAGAGTTAAAGTTTCGGCAAACCGAGTAGCCACTGTTGTTGCTAATGAGTGGAAGGATGAAAATGACAAAGTTATGCCTGTGAAACAGTGGCTGGAAGACAGAAGATTAATGCAGGCAGAGATGCAACGCATGAGGGAGAAGCTAGCAATCTCGGATAGAACAGCCAAGGCAGAGGCACAACTCAAGGAAAAGCTGAAGTTGAGGCTGAAGACTCTCGAAGAAGGATTAAAGCATGTTTCGAGTTTCTCAGTCAATCCTAATTCGTTTTGCCGTTCCCCTAAAACAGAAAAGTCCAGTAACTTCTTAGGATTTCTGACAAGCCCTGGTGGATTAGGAAAGAGATCAACTTCACAGCCAAGAGCCTCCTCACTTAGAAGCTCCCCTCTCCAACAACCAAATTCAGACAATGAAACAGCCAATGCTGCCGGAGACCTAAAAAGATCAAATAGCTTTAAAAATAGATATGGTTCAGGAGAAAAAATGCTGAGAAAAAGCTTATGGGCTTCTAGAACTAAAGTTGTTGACAGTGGTGAGAAGGAGAACACAGAAGGGAAGACAAATACTGATAACAACAAGAATGATGATAAAACTGTTTCTGCTGATATTAAAACTAAAGATGGCGCCGATGAAGACTCGCCTAGCAAGATAAGTATTAACTGTGAAAGTGAGGATGTGGTCTCAGGATTTCTGTATGACAGACTACAAAAGGAGGTCATCAATCTGAGGAGGCATTGTGAAGTCAAAGAGACTGATATGACTGCGAAAGAACAAGAAATAAAGTTGCTGATGAAAAAGGTTGATGCACTGACAAAAGCCATGGAAGTGGAGTCTAAGAGAATGAAGAGAGAAGCCATAGCTAGAGATAAAGAAGCTGCAGCAGCCAAGGTGGATGATAACAAAAAGAACAGAACTATTAACTCATCCAAGAGGGTAACAAAAGCAGGTTGA

Protein Analysis

583

Amino Acids

65.8

Weight (kDa)

8.71

Isoelectric Point (pI)

46.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MAP70 PF07058 21 - 576 6.5e-220 Microtubule-associated protein 70
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015178)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G17220
fragaria_vesca FvH4_3g00480 FvH4_3g00480
malus_domestica MD00G1056800.v1.1
prunus_persica Prupe.4G005400_v2.0.a1
pyrus_communis pycom05g32710
rosa_chinensis RchiOBHm_Chr5g0000651
rosa_laevigata RLG00000030885
rosa_multiflora Rmu_sc0011992.1_g000012
rosa_roxburghii Rroxscaffold_1G00075550
rosa_rugosa Rorug04G0385500
rosa_samantha Rh5AG005300 Rh5BG006900 Rh5CG005900 Rh5DG005800
rosa_wichuraiana Rw5G000550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 80
Acc36I ACCTGC 1 cut(s) 1733
AccB1I GGYRCC 1 cut(s) 1421
AciI CCGC 1 cut(s) 8
AcsI RAATTY 1 cut(s) 1198
AcuI CTGAAG 4 cut(s) 195, 437, 1016, 1028
AcyI GRCGYC 1 cut(s) 1422
AfaI GTAC 2 cut(s) 111, 354
AflII CTTAAG 1 cut(s) 182
AgsI TTSAA 9 cut(s) 278, 392, 542, 560, 569, 584, 602, 665, 686
AjnI CCWGG 2 cut(s) 609, 1123
AjuI GAANNNNNNNTTGG 2 cut(s) 116, 148
Alw21I GWGCWC 1 cut(s) 538
Alw26I GTCTC 4 cut(s) 586, 1224, 1483, 1541
Alw44I GTGCAC 1 cut(s) 534
AlwNI CAGNNNCTG 2 cut(s) 77, 892
Ama87I CYCGRG 1 cut(s) 410
AoxI GGCC 1 cut(s) 699
ApaLI GTGCAC 1 cut(s) 534
ApoI RAATTY 1 cut(s) 1198
AseI ATTAAT 1 cut(s) 908
Asp700I GAANNNNTTC 1 cut(s) 564
AspLEI GCGC 1 cut(s) 1424
AspS9I GGNCC 1 cut(s) 52
AsuHPI GGTGA 2 cut(s) 161, 1343
AsuNHI GCTAGC 2 cut(s) 796, 943
AvaI CYCGRG 1 cut(s) 410
AvaII GGWCC 1 cut(s) 52
BaeGI GKGCMC 1 cut(s) 538
BanI GGYRCC 1 cut(s) 1421
BbsI GAAGAC 5 cut(s) 785, 903, 1016, 1361, 1437
Bbv12I GWGCWC 1 cut(s) 538
BccI CCATC 1 cut(s) 1412
BceAI ACGGC 1 cut(s) 1056
BciT130I CCWGG 2 cut(s) 611, 1125
BcoDI GTCTC 4 cut(s) 586, 1224, 1483, 1541
BfaI CTAG 6 cut(s) 260, 797, 944, 1308, 1440, 1659
BfmI CTRYAG 4 cut(s) 69, 195, 330, 1674
BfoI RGCGCY 1 cut(s) 1425
BfrI CTTAAG 1 cut(s) 182
BfuAI ACCTGC 1 cut(s) 1733
BlpI GCTNAGC 1 cut(s) 671
Bme1390I CCNGG 2 cut(s) 611, 1125
Bme18I GGWCC 1 cut(s) 52
BmeT110I CYCGRG 1 cut(s) 410
BmgT120I GGNCC 1 cut(s) 52
BmiI GGNNCC 1 cut(s) 1423
BmrFI CCNGG 2 cut(s) 611, 1125
BmsI GCATC 3 cut(s) 298, 912, 1591
BmtI GCTAGC 2 cut(s) 800, 947
BpiI GAAGAC 5 cut(s) 785, 903, 1016, 1361, 1437
BpmI CTGGAG 2 cut(s) 732, 806
Bpu1102I GCTNAGC 1 cut(s) 671
BpuEI CTTGAG 5 cut(s) 129, 530, 650, 713, 968
BsaBI GATNNNNATC 2 cut(s) 375, 602
BsaHI GRCGYC 1 cut(s) 1422
BsaI GGTCTC 2 cut(s) 1224, 1483
BsaJI CCNNGG 4 cut(s) 966, 1123, 1617, 1683
BsaXI ACNNNNNCTCC 2 cut(s) 1519, 1549
Bse1I ACTGG 1 cut(s) 1095
Bse3DI GCAATG 1 cut(s) 399
Bse8I GATNNNNATC 2 cut(s) 375, 602
BseBI CCWGG 2 cut(s) 611, 1125
BseDI CCNNGG 4 cut(s) 966, 1123, 1617, 1683
BseGI GGATG 6 cut(s) 235, 376, 862, 1477, 1696, 1721
BseJI GATNNNNATC 2 cut(s) 375, 602
BseMI GCAATG 1 cut(s) 399
BseMII CTCAG 5 cut(s) 178, 1062, 1277, 1494, 1514
BseNI ACTGG 1 cut(s) 1095
BseRI GAGGAG 5 cut(s) 54, 57, 710, 1156, 1540
BseSI GKGCMC 1 cut(s) 538
BsgI GTGCAG 1 cut(s) 303
BshFI GGCC 1 cut(s) 701
BshNI GGYRCC 1 cut(s) 1421
BsiHKAI GWGCWC 1 cut(s) 538
BsiHKCI CYCGRG 1 cut(s) 410
BsiSI CCGG 1 cut(s) 1227
BsmAI GTCTC 4 cut(s) 586, 1224, 1483, 1541
BsnI GGCC 1 cut(s) 701
Bso31I GGTCTC 2 cut(s) 1224, 1483
BsoBI CYCGRG 1 cut(s) 410
Bsp1286I GDGCHC 1 cut(s) 538
Bsp143I GATC 4 cut(s) 153, 376, 1142, 1241
Bsp1720I GCTNAGC 1 cut(s) 671
Bsp19I CCATGG 1 cut(s) 1617
BspACI CCGC 1 cut(s) 8
BspANI GGCC 1 cut(s) 701
BspCNI CTCAG 5 cut(s) 177, 1061, 1278, 1493, 1515
BspLI GGNNCC 1 cut(s) 1423
BspMAI CTGCAG 4 cut(s) 73, 199, 334, 1678
BspMI ACCTGC 1 cut(s) 1733
BspOI GCTAGC 2 cut(s) 800, 947
BspQI GCTCTTC 2 cut(s) 195, 510
BspT107I GGYRCC 1 cut(s) 1421
BspTI CTTAAG 1 cut(s) 182
BspTNI GGTCTC 2 cut(s) 1224, 1483
BsrDI GCAATG 1 cut(s) 399
BsrI ACTGG 1 cut(s) 1095
BssECI CCNNGG 4 cut(s) 966, 1123, 1617, 1683
BssMI GATC 4 cut(s) 153, 376, 1142, 1241
BssNI GRCGYC 1 cut(s) 1422
BssT1I CCWWGG 3 cut(s) 966, 1617, 1683
Bst2UI CCWGG 2 cut(s) 611, 1125
Bst4CI ACNGT 5 cut(s) 835, 888, 1328, 1393, 1460
Bst6I CTCTTC 5 cut(s) 195, 261, 510, 555, 1637
BstACI GRCGYC 1 cut(s) 1422
BstAFI CTTAAG 1 cut(s) 182
BstAPI GCANNNNNTGC 2 cut(s) 317, 326
BstC8I GCNNGC 4 cut(s) 101, 798, 915, 945
BstDSI CCRYGG 1 cut(s) 1617
BstF5I GGATG 6 cut(s) 235, 376, 862, 1477, 1696, 1721
BstH2I RGCGCY 1 cut(s) 1425
BstHHI GCGC 1 cut(s) 1424
BstKTI GATC 4 cut(s) 156, 379, 1145, 1244
BstMAI GTCTC 4 cut(s) 586, 1224, 1483, 1541
BstMBI GATC 4 cut(s) 153, 376, 1142, 1241
BstMWI GCNNNNNNNGC 9 cut(s) 167, 317, 326, 335, 707, 716, 728, 1223, 1679
BstNI CCWGG 2 cut(s) 611, 1125
BstNSI RCATGY 2 cut(s) 103, 1037
BstSCI CCNGG 2 cut(s) 609, 1123
BstSFI CTRYAG 4 cut(s) 69, 195, 330, 1674
BstSLI GKGCMC 1 cut(s) 538
BstV2I GAAGAC 5 cut(s) 785, 903, 1016, 1361, 1437
BstXI CCANNNNNNTGG 1 cut(s) 1624
BsuRI GGCC 1 cut(s) 701
BtgI CCRYGG 1 cut(s) 1617
BtsCI GGATG 6 cut(s) 235, 376, 862, 1477, 1696, 1721
BtsI GCAGTG 1 cut(s) 66
BtsIMutI CAGTG 5 cut(s) 66, 831, 893, 1333, 1604
BveI ACCTGC 1 cut(s) 1733
Cac8I GCNNGC 4 cut(s) 101, 798, 915, 945
CaiI CAGNNNCTG 2 cut(s) 77, 892
CfoI GCGC 1 cut(s) 1424
Cfr13I GGNCC 1 cut(s) 52
Csp6I GTAC 2 cut(s) 110, 353
CspCI CAANNNNNGTGG 2 cut(s) 820, 855
CviAII CATG 5 cut(s) 100, 767, 931, 1034, 1618
CviQI GTAC 2 cut(s) 110, 353
DinI GGCGCC 1 cut(s) 1423
DpnI GATC 4 cut(s) 155, 378, 1144, 1243
DpnII GATC 4 cut(s) 153, 376, 1142, 1241
DraI TTTAAA 1 cut(s) 1255
DrdI GACNNNNNNGTC 1 cut(s) 80
DseDI GACNNNNNNGTC 1 cut(s) 80
Eam1104I CTCTTC 5 cut(s) 195, 261, 510, 555, 1637
EarI CTCTTC 5 cut(s) 195, 261, 510, 555, 1637
Eco130I CCWWGG 3 cut(s) 966, 1617, 1683
Eco31I GGTCTC 2 cut(s) 1224, 1483
Eco47I GGWCC 1 cut(s) 52
Eco57I CTGAAG 4 cut(s) 195, 437, 1016, 1028
Eco88I CYCGRG 1 cut(s) 410
EcoRII CCWGG 2 cut(s) 609, 1123
EcoT14I CCWWGG 3 cut(s) 966, 1617, 1683
EcoT22I ATGCAT 1 cut(s) 313
EgeI GGCGCC 1 cut(s) 1423
EheI GGCGCC 1 cut(s) 1423
ErhI CCWWGG 3 cut(s) 966, 1617, 1683
FaeI CATG 5 cut(s) 103, 770, 934, 1037, 1621
FalI AAGNNNNNCTT 2 cut(s) 327, 359
FatI CATG 5 cut(s) 99, 766, 930, 1033, 1617
FokI GGATG 6 cut(s) 242, 383, 869, 1484, 1703, 1708
FspBI CTAG 6 cut(s) 260, 797, 944, 1308, 1440, 1659
GlaI GCGC 1 cut(s) 1423
GsuI CTGGAG 2 cut(s) 732, 806
HaeII RGCGCY 1 cut(s) 1425
HaeIII GGCC 1 cut(s) 701
HapII CCGG 1 cut(s) 1227
HhaI GCGC 1 cut(s) 1424
Hin1I GRCGYC 1 cut(s) 1422
Hin1II CATG 5 cut(s) 103, 770, 934, 1037, 1621
Hin6I GCGC 1 cut(s) 1422
HinP1I GCGC 1 cut(s) 1422
HincII GTYRAC 1 cut(s) 1324
HindII GTYRAC 1 cut(s) 1324
HindIII AAGCTT 2 cut(s) 668, 1294
HinfI GANTC 8 cut(s) 124, 243, 392, 624, 781, 1012, 1433, 1628
HpaII CCGG 1 cut(s) 1227
HphI GGTGA 2 cut(s) 161, 1343
Hpy166II GTNNAC 3 cut(s) 355, 536, 1324
Hpy188I TCNGA 5 cut(s) 167, 955, 1116, 1204, 1524
Hpy8I GTNNAC 3 cut(s) 355, 536, 1324
HpyAV CCTTC 7 cut(s) 342, 393, 460, 847, 1016, 1330, 1343
HpyCH4III ACNGT 5 cut(s) 835, 888, 1328, 1393, 1460
HpyCH4IV ACGT 2 cut(s) 108, 478
HpyF10VI GCNNNNNNNGC 9 cut(s) 167, 317, 326, 335, 707, 716, 728, 1223, 1679
HpySE526I ACGT 2 cut(s) 108, 478
Hsp92I GRCGYC 1 cut(s) 1422
Hsp92II CATG 5 cut(s) 103, 770, 934, 1037, 1621
HspAI GCGC 1 cut(s) 1422
KasI GGCGCC 1 cut(s) 1421
Kzo9I GATC 4 cut(s) 153, 376, 1142, 1241
LguI GCTCTTC 2 cut(s) 195, 510
LmnI GCTCC 4 cut(s) 44, 409, 793, 1184
LweI GCATC 3 cut(s) 298, 912, 1591
MaeI CTAG 6 cut(s) 260, 797, 944, 1308, 1440, 1659
MaeII ACGT 2 cut(s) 108, 478
MaeIII GTNAC 4 cut(s) 64, 737, 1097, 1732
MalI GATC 4 cut(s) 155, 378, 1144, 1243
MboI GATC 4 cut(s) 153, 376, 1142, 1241
MhlI GDGCHC 1 cut(s) 538
MluCI AATT 5 cut(s) 497, 584, 761, 1060, 1198
Mly113I GGCGCC 1 cut(s) 1422
MlyI GAGTC 5 cut(s) 237, 775, 1006, 1427, 1637
MmeI TCCRAC 1 cut(s) 1213
Mph1103I ATGCAT 1 cut(s) 313
MroXI GAANNNNTTC 1 cut(s) 564
MslI CAYNNNNRTG 1 cut(s) 1622
MspCI CTTAAG 1 cut(s) 182
MspI CCGG 1 cut(s) 1227
MspR9I CCNGG 2 cut(s) 611, 1125
MvaI CCWGG 2 cut(s) 611, 1125
MwoI GCNNNNNNNGC 9 cut(s) 167, 317, 326, 335, 707, 716, 728, 1223, 1679
NarI GGCGCC 1 cut(s) 1422
NcoI CCATGG 1 cut(s) 1617
NdeII GATC 4 cut(s) 153, 376, 1142, 1241
NheI GCTAGC 2 cut(s) 796, 943
NlaIII CATG 5 cut(s) 103, 770, 934, 1037, 1621
NlaIV GGNNCC 1 cut(s) 1423
NmuCI GTSAC 2 cut(s) 64, 737
NsiI ATGCAT 1 cut(s) 313
NspI RCATGY 2 cut(s) 103, 1037
PaeI GCATGC 1 cut(s) 103
PaeR7I CTCGAG 1 cut(s) 410
PciSI GCTCTTC 2 cut(s) 195, 510
PdmI GAANNNNTTC 1 cut(s) 564
PfeI GAWTC 3 cut(s) 124, 392, 624
PflFI GACNNNGTC 1 cut(s) 77
PleI GAGTC 5 cut(s) 237, 775, 1006, 1427, 1636
PluTI GGCGCC 1 cut(s) 1425
PpsI GAGTC 5 cut(s) 237, 775, 1006, 1427, 1636
PshBI ATTAAT 1 cut(s) 908
Psp6I CCWGG 2 cut(s) 609, 1123
PspGI CCWGG 2 cut(s) 609, 1123
PspN4I GGNNCC 1 cut(s) 1423
PspPI GGNCC 1 cut(s) 52
PspXI VCTCGAGB 1 cut(s) 410
PsrI GAACNNNNNNTAC 2 cut(s) 9, 41
PstI CTGCAG 4 cut(s) 73, 199, 334, 1678
PstNI CAGNNNCTG 2 cut(s) 77, 892
PsyI GACNNNGTC 1 cut(s) 77
RsaI GTAC 2 cut(s) 111, 354
RsaNI GTAC 2 cut(s) 110, 353
RseI CAYNNNNRTG 1 cut(s) 1622
SapI GCTCTTC 2 cut(s) 195, 510
Sau3AI GATC 4 cut(s) 153, 376, 1142, 1241
Sau96I GGNCC 1 cut(s) 52
SchI GAGTC 5 cut(s) 237, 775, 1006, 1427, 1637
ScrFI CCNGG 2 cut(s) 611, 1125
SduI GDGCHC 1 cut(s) 538
SfaNI GCATC 3 cut(s) 298, 912, 1591
SfcI CTRYAG 4 cut(s) 69, 195, 330, 1674
SfoI GGCGCC 1 cut(s) 1423
Sfr274I CTCGAG 1 cut(s) 410
SinI GGWCC 1 cut(s) 52
SlaI CTCGAG 1 cut(s) 410
SmiMI CAYNNNNRTG 1 cut(s) 1622
SmlI CTYRAG 7 cut(s) 144, 182, 410, 509, 629, 692, 983
SmoI CTYRAG 7 cut(s) 144, 182, 410, 509, 629, 692, 983
SphI GCATGC 1 cut(s) 103
Sse9I AATT 5 cut(s) 497, 584, 761, 1060, 1198
SsiI CCGC 1 cut(s) 8
SspDI GGCGCC 1 cut(s) 1421
SspMI CTAG 6 cut(s) 260, 797, 944, 1308, 1440, 1659
StyD4I CCNGG 2 cut(s) 609, 1123
StyI CCWWGG 3 cut(s) 966, 1617, 1683
TaaI ACNGT 5 cut(s) 835, 888, 1328, 1393, 1460
TaiI ACGT 2 cut(s) 111, 481
TaqI TCGA 5 cut(s) 364, 411, 491, 1017, 1040
TasI AATT 5 cut(s) 497, 584, 761, 1060, 1198
TfiI GAWTC 3 cut(s) 124, 392, 624
TscAI CASTG 5 cut(s) 73, 838, 893, 1333, 1611
TseFI GTSAC 2 cut(s) 64, 737
Tsp45I GTSAC 2 cut(s) 64, 737
TspDTI ATGAA 7 cut(s) 228, 774, 873, 1224, 1443, 1604, 1655
TspRI CASTG 5 cut(s) 73, 838, 893, 1333, 1611
Tth111I GACNNNGTC 1 cut(s) 77
Vha464I CTTAAG 1 cut(s) 182
VneI GTGCAC 1 cut(s) 534
VpaK11BI GGWCC 1 cut(s) 52
VspI ATTAAT 1 cut(s) 908
XapI RAATTY 1 cut(s) 1198
XbaI TCTAGA 2 cut(s) 259, 1307
XceI RCATGY 2 cut(s) 103, 1037
XhoI CTCGAG 1 cut(s) 410
XmnI GAANNNNTTC 1 cut(s) 564
XspI CTAG 6 cut(s) 260, 797, 944, 1308, 1440, 1659
Zsp2I ATGCAT 1 cut(s) 313
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.