AT4G17260

L-lactate dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0005141
Physical Location & Seq
Forward (+)
9673897 .. 9675540
1644 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G17260.1

Sequence Viewer

Length: 1062 bp
ATGGAGAAGAACGCATCGACTTCGTCGTTGAAGGACTTGGGTCCGAGTGGTCTAGATCTAACCAGCGCCTTTTTCAAACCGATCCACAACTCCGATCCTTCTCTCCCTTCAAACCGACGTACCAAAGTCTCCGTCGTCGGAGTCGGAAACGTCGGAATGGCCATAGCTCAAACCATCCTCACTCAAGATCTCGCAGACGAGATCGCTCTCGTTGACGCCAAACCCGATAAGCTTCGTGGCGAAATGCTTGATCTCCAACACGCCGCTGCTTTCCTCCCTCGAACTAAGATCACCGCTTCTGTTGATTACGAAGTCACCGCTGGATCTGATCTTTGTATCGTCACTGCTGGTGCTAGACAGAATCCAGGCGAGTCTAGACTCAATTTGCTTCAGAGGAACGTCGCTCTCTTCCGTCATATCATTCCTCCACTCGCTAAAGCTTCTCCTGATTCTATATTGATCATTGTTTCTAATCCCGTTGATGTTTTGACTTACGTTGCTTGGAAACTCTCTGGCTTTCCGGTGAATCGGGTGCTTGGATCTGGTACTAATCTCGATTCTTCTCGGTTCCGATTCTTAATCGCAGATCATCTCGACGTTAATGCTCAGGATGTACAGGCATTTATTGTGGGAGAGCATGGAGACAGCTCAGTGGCATTGTGGTCAAGCATTAGTGTTGGAGGCATTCCTGTCTTAAGCTTTTTGGAGAAGAACCAGATAGCTTACGAGAAACAAACTCTTGAGGATATCCACCAAGCTGTTGTTGGTAGCGCCTATGAAGTGATTGGACTCAAGGGTTACACTTCTTGGGCCATTGGCTACTCAGTTGCTAATCTGGCTCGTACTATCCTCCGTGACCAGCGCAAGATCCACCCGGTTACTGTTCTTGCTCGTGGATTTTATGGTGTTGATGGTGGTGATGTTTTCCTCAGTCTTCCGGCTTTGCTTGGAAGGAATGGTGTGGTTGCTGTGACCAATGTGCATATGACTGATGAAGAGGCTGAGAAGCTGCAGAAATCGGCAAAGACTATATTGGAGATGCAGAGCCAATTGGGACTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko00270 ko00620 ko00640 ko01100 ko01110 ko01120 ko01130 ko04922 map00010 map00270 map00620 map00640 map01100 map01110 map01120 map01130 map04922
Pfam Domains
Protein Families
Ldh_1_C Ldh_1_N

Protein Analysis

353

Amino Acids

37.95

Weight (kDa)

6.07

Isoelectric Point (pI)

28.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ldh_1_N PF00056 41 - 180 4.4e-44 lactate/malate dehydrogenase, NAD binding domain
Ldh_1_C PF02866 183 - 346 1.8e-32 lactate/malate dehydrogenase, alpha/beta C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 264, 294, 318
AclWI GGATC 5 cut(s) 76, 89, 331, 547, 862
AcoI YGGCCR 1 cut(s) 159
AcuI CTGAAG 1 cut(s) 374
AcyI GRCGYC 1 cut(s) 216
AfaI GTAC 4 cut(s) 121, 547, 615, 844
AflII CTTAAG 1 cut(s) 694
AgsI TTSAA 3 cut(s) 31, 76, 111
AjnI CCWGG 1 cut(s) 364
AluBI AGCT 8 cut(s) 167, 232, 440, 648, 699, 722, 758, 1009
AluI AGCT 8 cut(s) 167, 232, 440, 648, 699, 722, 758, 1009
Alw26I GTCTC 2 cut(s) 133, 636
AlwI GGATC 5 cut(s) 76, 89, 331, 547, 862
AoxI GGCC 2 cut(s) 159, 810
ApeKI GCWGC 2 cut(s) 266, 1009
ArsI GACNNNNNNTTYG 2 cut(s) 117, 149
AspLEI GCGC 3 cut(s) 68, 773, 864
AspS9I GGNCC 2 cut(s) 41, 810
AsuC2I CCSGG 1 cut(s) 875
AsuHPI GGTGA 4 cut(s) 283, 307, 535, 929
AvaII GGWCC 1 cut(s) 41
BaeI ACNNNNGTAYC 2 cut(s) 103, 136
BalI TGGCCA 1 cut(s) 161
BauI CACGAG 1 cut(s) 891
BbsI GAAGAC 1 cut(s) 926
BbvI GCAGC 2 cut(s) 253, 996
BccI CCATC 2 cut(s) 182, 905
BcgI CGANNNNNNTGC 3 cut(s) 37, 584, 618
BciT130I CCWGG 1 cut(s) 366
BclI TGATCA 1 cut(s) 459
BcnI CCSGG 1 cut(s) 875
BcoDI GTCTC 2 cut(s) 133, 636
BfaI CTAG 3 cut(s) 53, 354, 375
BfmI CTRYAG 1 cut(s) 1010
BfoI RGCGCY 2 cut(s) 69, 774
BfrI CTTAAG 1 cut(s) 694
BglII AGATCT 2 cut(s) 55, 187
BisI GCNGC 3 cut(s) 264, 267, 1010
BlsI GCNGC 3 cut(s) 265, 268, 1011
Bme1390I CCNGG 2 cut(s) 366, 875
Bme18I GGWCC 1 cut(s) 41
BmgT120I GGNCC 2 cut(s) 41, 810
BmiI GGNNCC 2 cut(s) 42, 569
BmrFI CCNGG 2 cut(s) 366, 875
BmsI GCATC 2 cut(s) 23, 1029
BoxI GACNNNNGTC 1 cut(s) 39
BpiI GAAGAC 1 cut(s) 926
Bpu10I CCTNAGC 1 cut(s) 606
BpuEI CTTGAG 3 cut(s) 168, 761, 776
BpuMI CCSGG 1 cut(s) 875
BsaHI GRCGYC 1 cut(s) 216
BsaWI WCCGGW 1 cut(s) 520
BseBI CCWGG 1 cut(s) 366
BseGI GGATG 2 cut(s) 174, 616
BseMII CTCAG 5 cut(s) 620, 663, 837, 943, 993
BseXI GCAGC 2 cut(s) 253, 996
BshFI GGCC 2 cut(s) 161, 812
BsiSI CCGG 3 cut(s) 521, 875, 938
BsmAI GTCTC 2 cut(s) 133, 636
BsmI GAATGC 1 cut(s) 684
BsnI GGCC 2 cut(s) 161, 812
Bsp1407I TGTACA 1 cut(s) 613
BspACI CCGC 3 cut(s) 264, 294, 318
BspANI GGCC 2 cut(s) 161, 812
BspCNI CTCAG 5 cut(s) 619, 662, 836, 942, 994
BspLI GGNNCC 2 cut(s) 42, 569
BspMAI CTGCAG 1 cut(s) 1014
BspPI GGATC 5 cut(s) 76, 89, 331, 547, 862
BspTI CTTAAG 1 cut(s) 694
BsrGI TGTACA 1 cut(s) 613
BssNI GRCGYC 1 cut(s) 216
BssSI CACGAG 1 cut(s) 891
Bst2BI CACGAG 1 cut(s) 891
Bst2UI CCWGG 1 cut(s) 366
Bst4CI ACNGT 1 cut(s) 883
Bst6I CTCTTC 2 cut(s) 413, 990
BstACI GRCGYC 1 cut(s) 216
BstAFI CTTAAG 1 cut(s) 694
BstAUI TGTACA 1 cut(s) 613
BstDEI CTNAG 6 cut(s) 285, 606, 649, 823, 929, 1002
BstF5I GGATG 2 cut(s) 174, 616
BstH2I RGCGCY 2 cut(s) 69, 774
BstHHI GCGC 3 cut(s) 68, 773, 864
BstMAI GTCTC 2 cut(s) 133, 636
BstMWI GCNNNNNNNGC 1 cut(s) 836
BstNI CCWGG 1 cut(s) 366
BstPAI GACNNNNGTC 1 cut(s) 39
BstSCI CCNGG 2 cut(s) 364, 873
BstSFI CTRYAG 1 cut(s) 1010
BstV1I GCAGC 2 cut(s) 253, 996
BstV2I GAAGAC 1 cut(s) 926
BstX2I RGATCY 5 cut(s) 55, 187, 323, 539, 867
BstYI RGATCY 5 cut(s) 55, 187, 323, 539, 867
BsuRI GGCC 2 cut(s) 161, 812
BtsCI GGATG 2 cut(s) 174, 616
BtsI GCAGTG 1 cut(s) 342
BtsIMutI CAGTG 2 cut(s) 342, 657
CfoI GCGC 3 cut(s) 68, 773, 864
Cfr13I GGNCC 2 cut(s) 41, 810
CseI GACGC 1 cut(s) 224
Csp6I GTAC 4 cut(s) 120, 546, 614, 843
CviAII CATG 1 cut(s) 638
CviQI GTAC 4 cut(s) 120, 546, 614, 843
DdeI CTNAG 6 cut(s) 285, 606, 649, 823, 929, 1002
EaeI YGGCCR 1 cut(s) 159
Eam1104I CTCTTC 2 cut(s) 413, 990
EarI CTCTTC 2 cut(s) 413, 990
Eco32I GATATC 1 cut(s) 748
Eco47I GGWCC 1 cut(s) 41
Eco57I CTGAAG 1 cut(s) 374
EcoRII CCWGG 1 cut(s) 364
EcoRV GATATC 1 cut(s) 748
FaeI CATG 1 cut(s) 641
FaiI YATR 9 cut(s) 164, 417, 455, 639, 777, 903, 984, 986, 1031
FatI CATG 1 cut(s) 637
FauNDI CATATG 1 cut(s) 984
FbaI TGATCA 1 cut(s) 459
Fnu4HI GCNGC 3 cut(s) 264, 267, 1010
FokI GGATG 2 cut(s) 161, 623
Fsp4HI GCNGC 3 cut(s) 264, 267, 1010
FspBI CTAG 3 cut(s) 53, 354, 375
GlaI GCGC 3 cut(s) 67, 772, 863
GluI GCNGC 3 cut(s) 264, 267, 1010
HaeII RGCGCY 2 cut(s) 69, 774
HaeIII GGCC 2 cut(s) 161, 812
HapII CCGG 3 cut(s) 521, 875, 938
HgaI GACGC 1 cut(s) 224
HhaI GCGC 3 cut(s) 68, 773, 864
Hin1I GRCGYC 1 cut(s) 216
Hin1II CATG 1 cut(s) 641
Hin6I GCGC 3 cut(s) 66, 771, 862
HinP1I GCGC 3 cut(s) 66, 771, 862
HincII GTYRAC 1 cut(s) 214
HindII GTYRAC 1 cut(s) 214
HindIII AAGCTT 3 cut(s) 230, 438, 697
HinfI GANTC 9 cut(s) 141, 361, 371, 378, 449, 526, 557, 573, 789
HpaII CCGG 3 cut(s) 521, 875, 938
HphI GGTGA 4 cut(s) 283, 307, 535, 929
Hpy166II GTNNAC 1 cut(s) 214
Hpy188I TCNGA 8 cut(s) 45, 94, 140, 146, 155, 328, 393, 572
Hpy188III TCNNGA 8 cut(s) 53, 185, 375, 446, 554, 593, 608, 740
Hpy8I GTNNAC 1 cut(s) 214
Hpy99I CGWCG 7 cut(s) 28, 120, 137, 140, 155, 404, 599
HpyAV CCTTC 4 cut(s) 25, 108, 117, 945
HpyCH4III ACNGT 1 cut(s) 883
HpyCH4IV ACGT 5 cut(s) 118, 150, 399, 495, 597
HpyCH4V TGCA 3 cut(s) 982, 1012, 1042
HpyF10VI GCNNNNNNNGC 1 cut(s) 836
HpyF3I CTNAG 6 cut(s) 285, 606, 649, 823, 929, 1002
HpySE526I ACGT 5 cut(s) 118, 150, 399, 495, 597
Hsp92I GRCGYC 1 cut(s) 216
Hsp92II CATG 1 cut(s) 641
HspAI GCGC 3 cut(s) 66, 771, 862
Ksp22I TGATCA 1 cut(s) 459
Lsp1109I GCAGC 2 cut(s) 253, 996
LweI GCATC 2 cut(s) 23, 1029
MaeI CTAG 3 cut(s) 53, 354, 375
MaeII ACGT 5 cut(s) 118, 150, 399, 495, 597
MaeIII GTNAC 6 cut(s) 313, 340, 797, 854, 877, 970
MboII GAAGA 6 cut(s) 19, 400, 552, 721, 926, 1007
MfeI CAATTG 1 cut(s) 1049
MflI RGATCY 5 cut(s) 55, 187, 323, 539, 867
MlsI TGGCCA 1 cut(s) 161
MluCI AATT 2 cut(s) 382, 1049
MluNI TGGCCA 1 cut(s) 161
MlyI GAGTC 4 cut(s) 150, 372, 380, 783
MmeI TCCRAC 5 cut(s) 118, 124, 133, 280, 658
Mox20I TGGCCA 1 cut(s) 161
MscI TGGCCA 1 cut(s) 161
MseI TTAA 3 cut(s) 578, 600, 695
Msp20I TGGCCA 1 cut(s) 161
MspA1I CMGCKG 2 cut(s) 266, 320
MspCI CTTAAG 1 cut(s) 694
MspI CCGG 3 cut(s) 521, 875, 938
MspR9I CCNGG 2 cut(s) 366, 875
MunI CAATTG 1 cut(s) 1049
Mva1269I GAATGC 1 cut(s) 684
MvaI CCWGG 1 cut(s) 366
MwoI GCNNNNNNNGC 1 cut(s) 836
NciI CCSGG 1 cut(s) 875
NdeI CATATG 1 cut(s) 984
NlaIII CATG 1 cut(s) 641
NlaIV GGNNCC 2 cut(s) 42, 569
NmuCI GTSAC 4 cut(s) 313, 340, 854, 970
PcsI WCGNNNNNNNCGW 2 cut(s) 23, 222
PctI GAATGC 1 cut(s) 684
PfeI GAWTC 5 cut(s) 361, 449, 526, 557, 573
PflFI GACNNNGTC 1 cut(s) 22
PkrI GCNGC 3 cut(s) 265, 268, 1011
PleI GAGTC 4 cut(s) 149, 372, 379, 783
PpsI GAGTC 4 cut(s) 149, 372, 379, 783
PshAI GACNNNNGTC 1 cut(s) 39
Psp6I CCWGG 1 cut(s) 364
PspGI CCWGG 1 cut(s) 364
PspN4I GGNNCC 2 cut(s) 42, 569
PspPI GGNCC 2 cut(s) 41, 810
PstI CTGCAG 1 cut(s) 1014
PsuI RGATCY 5 cut(s) 55, 187, 323, 539, 867
PsyI GACNNNGTC 1 cut(s) 22
RsaI GTAC 4 cut(s) 121, 547, 615, 844
RsaNI GTAC 4 cut(s) 120, 546, 614, 843
SaqAI TTAA 3 cut(s) 578, 600, 695
SatI GCNGC 3 cut(s) 264, 267, 1010
Sau96I GGNCC 2 cut(s) 41, 810
SchI GAGTC 4 cut(s) 150, 372, 380, 783
ScrFI CCNGG 2 cut(s) 366, 875
SfaNI GCATC 2 cut(s) 23, 1029
SfcI CTRYAG 1 cut(s) 1010
SinI GGWCC 1 cut(s) 41
SmlI CTYRAG 4 cut(s) 183, 694, 740, 791
SmoI CTYRAG 4 cut(s) 183, 694, 740, 791
Sse9I AATT 2 cut(s) 382, 1049
SsiI CCGC 3 cut(s) 264, 294, 318
SspMI CTAG 3 cut(s) 53, 354, 375
StyD4I CCNGG 2 cut(s) 364, 873
TaaI ACNGT 1 cut(s) 883
TaiI ACGT 5 cut(s) 121, 153, 402, 498, 600
TaqI TCGA 4 cut(s) 17, 280, 555, 594
TasI AATT 2 cut(s) 382, 1049
TatI WGTACW 1 cut(s) 613
TauI GCSGC 1 cut(s) 266
TfiI GAWTC 5 cut(s) 361, 449, 526, 557, 573
Tru1I TTAA 3 cut(s) 578, 600, 695
Tru9I TTAA 3 cut(s) 578, 600, 695
TscAI CASTG 2 cut(s) 349, 657
TseFI GTSAC 4 cut(s) 313, 340, 854, 970
TseI GCWGC 2 cut(s) 266, 1009
Tsp45I GTSAC 4 cut(s) 313, 340, 854, 970
TspDTI ATGAA 2 cut(s) 792, 1008
TspGWI ACGGA 3 cut(s) 121, 401, 842
TspRI CASTG 2 cut(s) 349, 657
Tth111I GACNNNGTC 1 cut(s) 22
Vha464I CTTAAG 1 cut(s) 694
VpaK11BI GGWCC 1 cut(s) 41
XbaI TCTAGA 2 cut(s) 52, 374
XcmI CCANNNNNNNNNTGG 1 cut(s) 761
XspI CTAG 3 cut(s) 53, 354, 375
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.