RchiOBHm_Chr4g0406091
ERF Family

Belongs to the LDH MDH superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0005141
Physical Location & Seq
Forward (+)
27181827 .. 27183145
1319 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ37752

Sequence Viewer

Length: 1053 bp
ATGAACAAAAGCAGTTCGGCTTCGTCATTGGGCCCTGGAGGCCTTGACCTAACCCAGGCCTTCTTCAGGCCCATCATCCGCGCAGCCCCGCCTTCCCCGACCAAGCGCCACAACAAAATCTCCGTCATTGGCGTCGGCAACGTCGGCATGGCCATAGCACAAACCATCTTAACCCTAGACCTCGCCGACGAGCTTGTCCTCGTCGACGCCAAACCTGAAAAGCTACGTGGAGAGATGCTGGACCTTCAACACGCAGCTGCCTTCTTACCCCGCACCAAAATCCACGCTTCCGTGGATTACGCCATCACCGTCGGATCCGACCTCTACATCGTGACGACCGGTGCGCGCCAGAACCCTGGCGAGTCCAGGCTGAACCTGCTGCAGAGGAACGTGGCTCTCTTTAGAAATATCATACCTCCACTAGTTAAGTACTCTCCGGACACCATATTACTCATCGTGTCCAATCCAGTGGATGTCTTGACTTACGTGGCGTGGAAGCTATCCGGATTTCTGAGCAACAGGGTTTTCGGGTCGAGTACGAATCTCGACTCATCTAGATTTCGGTTCCTCATGGTCGATCACCTGGACGTTAACGCTCAGGATGCTTACATCATTGGCGAGCACGGGGACAGCTCAGTGGCTTTGTGGTCAAGCATCAGCGTCGGGGGAGTGCCGGTGTTGAGCTTCCTAGAGATGCACCAAATAGCATACGAGAAAGAGACACTGGAGAAGATTCACAAAGCAGTTGTGGACTGTGCGTATGAGGTGATAAGTCTGAAAGGGTATACTTCATGGGCAATTGGTTATTCAGTGGCAAACCTAGCCCGGAGCATACTCAGAGACCAGAGGAAGGTCCAACCTGTTTCAGTGCTTGCGAATGGGTTCTACGGTGTTGGTGGCGGTGATGTGTTCTTGAGCTTGCCTGCACAGCTCGGAAGGAGTGGAGTTCTGGGAGTGACCAATGTGCATTTGACTGATGAGGAGGCACAGAAGCTAAGGGACTCGGCTCACACCATTTTGGAGGTCCAAAGTCAGTTAAACTTGGGAATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko00270 ko00620 ko00640 ko01100 ko01110 ko01120 ko01130 ko04922 map00010 map00270 map00620 map00640 map01100 map01110 map01120 map01130 map04922
Pfam Domains
Protein Families
Ldh_1_C Ldh_1_N

Protein Analysis

350

Amino Acids

37.9

Weight (kDa)

6.76

Isoelectric Point (pI)

37.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ldh_1_N PF00056 39 - 177 1.9e-41 lactate/malate dehydrogenase, NAD binding domain
Ldh_1_C PF02866 180 - 341 4.6e-28 lactate/malate dehydrogenase, alpha/beta C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 194
Acc36I ACCTGC 1 cut(s) 384
AccI GTMKAC 2 cut(s) 204, 785
AccII CGCG 2 cut(s) 81, 346
AccIII TCCGGA 2 cut(s) 436, 503
AciI CCGC 4 cut(s) 79, 89, 271, 900
AclWI GGATC 2 cut(s) 309, 322
AcoI YGGCCR 1 cut(s) 150
AcuI CTGAAG 1 cut(s) 49
AcyI GRCGYC 2 cut(s) 132, 207
AfaI GTAC 2 cut(s) 431, 538
AfiI CCNNNNNNNGG 3 cut(s) 55, 66, 850
AgeI ACCGGT 1 cut(s) 338
AgsI TTSAA 1 cut(s) 248
AhlI ACTAGT 1 cut(s) 421
AjnI CCWGG 5 cut(s) 34, 54, 355, 365, 582
AluBI AGCT 9 cut(s) 193, 223, 257, 499, 633, 684, 918, 931, 994
AluI AGCT 9 cut(s) 193, 223, 257, 499, 633, 684, 918, 931, 994
Alw21I GWGCWC 1 cut(s) 624
Alw26I GTCTC 2 cut(s) 713, 834
AlwI GGATC 2 cut(s) 309, 322
Aor13HI TCCGGA 2 cut(s) 436, 503
AoxI GGCC 5 cut(s) 31, 40, 57, 68, 150
ApaI GGGCCC 1 cut(s) 35
ApeKI GCWGC 4 cut(s) 83, 254, 257, 379
ArsI GACNNNNNNTTYG 2 cut(s) 108, 140
AsiGI ACCGGT 1 cut(s) 338
Asp700I GAANNNNTTC 1 cut(s) 881
AspLEI GCGC 4 cut(s) 83, 108, 346, 348
AspS9I GGNCC 6 cut(s) 31, 32, 69, 241, 853, 1024
AsuC2I CCSGG 1 cut(s) 826
AsuHPI GGTGA 4 cut(s) 298, 572, 778, 914
AvaII GGWCC 3 cut(s) 241, 853, 1024
BaeGI GKGCMC 1 cut(s) 35
BalI TGGCCA 1 cut(s) 152
BamHI GGATCC 1 cut(s) 314
BanII GRGCYC 1 cut(s) 35
Bbv12I GWGCWC 1 cut(s) 624
BbvI GCAGC 4 cut(s) 95, 244, 266, 366
BccI CCATC 3 cut(s) 80, 173, 311
BcgI CGANNNNNNTGC 2 cut(s) 643, 677
BciT130I CCWGG 5 cut(s) 36, 56, 357, 367, 584
BcnI CCSGG 1 cut(s) 826
BcoDI GTCTC 2 cut(s) 713, 834
BcuI ACTAGT 1 cut(s) 421
BfaI CTAG 5 cut(s) 176, 422, 555, 689, 821
BfmI CTRYAG 1 cut(s) 380
BfoI RGCGCY 1 cut(s) 109
BfuAI ACCTGC 1 cut(s) 384
BglI GCCNNNNNGGC 1 cut(s) 39
BisI GCNGC 4 cut(s) 84, 255, 258, 380
BlsI GCNGC 4 cut(s) 85, 256, 259, 381
BmcAI AGTACT 1 cut(s) 431
Bme1390I CCNGG 6 cut(s) 36, 56, 357, 367, 584, 826
Bme18I GGWCC 3 cut(s) 241, 853, 1024
BmgT120I GGNCC 6 cut(s) 31, 32, 69, 241, 853, 1024
BmiI GGNNCC 3 cut(s) 33, 316, 566
BmrFI CCNGG 6 cut(s) 36, 56, 357, 367, 584, 826
BmsI GCATC 4 cut(s) 225, 592, 663, 684
BpmI CTGGAG 2 cut(s) 57, 746
Bpu10I CCTNAGC 2 cut(s) 597, 995
BpuEI CTTGAG 1 cut(s) 934
BpuMI CCSGG 1 cut(s) 826
BsaAI YACGTR 2 cut(s) 227, 487
BsaHI GRCGYC 2 cut(s) 132, 207
BsaI GGTCTC 1 cut(s) 834
BsaJI CCNNGG 4 cut(s) 34, 54, 291, 355
BsaWI WCCGGW 3 cut(s) 338, 436, 503
BsaXI ACNNNNNCTCC 4 cut(s) 104, 134, 660, 690
Bsc4I CCNNNNNNNGG 3 cut(s) 55, 66, 850
Bse118I RCCGGY 2 cut(s) 338, 673
Bse1I ACTGG 2 cut(s) 467, 729
BseAI TCCGGA 2 cut(s) 436, 503
BseBI CCWGG 5 cut(s) 36, 56, 357, 367, 584
BseDI CCNNGG 4 cut(s) 34, 54, 291, 355
BseGI GGATG 3 cut(s) 75, 478, 607
BseLI CCNNNNNNNGG 3 cut(s) 55, 66, 850
BseMII CTCAG 4 cut(s) 503, 611, 648, 850
BseNI ACTGG 2 cut(s) 467, 729
BsePI GCGCGC 1 cut(s) 344
BseRI GAGGAG 1 cut(s) 995
BseSI GKGCMC 1 cut(s) 35
BseXI GCAGC 4 cut(s) 95, 244, 266, 366
BsgI GTGCAG 1 cut(s) 909
Bsh1236I CGCG 2 cut(s) 81, 346
Bsh1285I CGRYCG 1 cut(s) 339
BshFI GGCC 5 cut(s) 33, 42, 59, 70, 152
BshTI ACCGGT 1 cut(s) 338
BsiEI CGRYCG 1 cut(s) 339
BsiHKAI GWGCWC 1 cut(s) 624
BsiSI CCGG 5 cut(s) 339, 437, 504, 674, 826
BslFI GGGAC 2 cut(s) 641, 1013
BslI CCNNNNNNNGG 3 cut(s) 55, 66, 850
BsmAI GTCTC 2 cut(s) 713, 834
BsmFI GGGAC 2 cut(s) 641, 1013
BsnI GGCC 5 cut(s) 33, 42, 59, 70, 152
Bso31I GGTCTC 1 cut(s) 834
Bsp120I GGGCCC 1 cut(s) 31
Bsp1286I GDGCHC 2 cut(s) 35, 624
Bsp13I TCCGGA 2 cut(s) 436, 503
Bsp143I GATC 2 cut(s) 314, 577
BspACI CCGC 4 cut(s) 79, 89, 271, 900
BspANI GGCC 5 cut(s) 33, 42, 59, 70, 152
BspCNI CTCAG 4 cut(s) 504, 610, 647, 849
BspEI TCCGGA 2 cut(s) 436, 503
BspFNI CGCG 2 cut(s) 81, 346
BspLI GGNNCC 3 cut(s) 33, 316, 566
BspMAI CTGCAG 1 cut(s) 384
BspMI ACCTGC 1 cut(s) 384
BspPI GGATC 2 cut(s) 309, 322
BspTNI GGTCTC 1 cut(s) 834
BsrFI RCCGGY 2 cut(s) 338, 673
BsrI ACTGG 2 cut(s) 467, 729
BssAI RCCGGY 2 cut(s) 338, 673
BssECI CCNNGG 4 cut(s) 34, 54, 291, 355
BssHII GCGCGC 1 cut(s) 344
BssMI GATC 2 cut(s) 314, 577
BssNAI GTATAC 1 cut(s) 786
BssNI GRCGYC 2 cut(s) 132, 207
Bst1107I GTATAC 1 cut(s) 786
Bst2UI CCWGG 5 cut(s) 36, 56, 357, 367, 584
Bst4CI ACNGT 3 cut(s) 310, 755, 890
BstACI GRCGYC 2 cut(s) 132, 207
BstBAI YACGTR 2 cut(s) 227, 487
BstC8I GCNNGC 5 cut(s) 346, 620, 873, 920, 924
BstDEI CTNAG 5 cut(s) 512, 597, 634, 836, 995
BstDSI CCRYGG 1 cut(s) 291
BstENI CCTNNNNNAGG 2 cut(s) 53, 64
BstF5I GGATG 3 cut(s) 75, 478, 607
BstFNI CGCG 2 cut(s) 81, 346
BstH2I RGCGCY 1 cut(s) 109
BstHHI GCGC 4 cut(s) 83, 108, 346, 348
BstKTI GATC 2 cut(s) 317, 580
BstMAI GTCTC 2 cut(s) 713, 834
BstMBI GATC 2 cut(s) 314, 577
BstMCI CGRYCG 1 cut(s) 339
BstMWI GCNNNNNNNGC 6 cut(s) 39, 144, 376, 602, 821, 928
BstNI CCWGG 5 cut(s) 36, 56, 357, 367, 584
BstSCI CCNGG 6 cut(s) 34, 54, 355, 365, 582, 824
BstSFI CTRYAG 1 cut(s) 380
BstSLI GKGCMC 1 cut(s) 35
BstUI CGCG 2 cut(s) 81, 346
BstV1I GCAGC 4 cut(s) 95, 244, 266, 366
BstX2I RGATCY 1 cut(s) 314
BstXI CCANNNNNNTGG 2 cut(s) 356, 469
BstYI RGATCY 1 cut(s) 314
BstZ17I GTATAC 1 cut(s) 786
BsuRI GGCC 5 cut(s) 33, 42, 59, 70, 152
BtgI CCRYGG 1 cut(s) 291
BtsCI GGATG 3 cut(s) 75, 478, 607
BtsIMutI CAGTG 5 cut(s) 474, 642, 722, 816, 873
BveI ACCTGC 1 cut(s) 384
Cac8I GCNNGC 5 cut(s) 346, 620, 873, 920, 924
CfoI GCGC 4 cut(s) 83, 108, 346, 348
Cfr10I RCCGGY 2 cut(s) 338, 673
Cfr13I GGNCC 6 cut(s) 31, 32, 69, 241, 853, 1024
CseI GACGC 3 cut(s) 121, 215, 649
Csp6I GTAC 2 cut(s) 430, 537
CspAI ACCGGT 1 cut(s) 338
CviAII CATG 3 cut(s) 148, 571, 792
CviQI GTAC 2 cut(s) 430, 537
DdeI CTNAG 5 cut(s) 512, 597, 634, 836, 995
DpnI GATC 2 cut(s) 316, 579
DpnII GATC 2 cut(s) 314, 577
DrdI GACNNNNNNGTC 1 cut(s) 194
DseDI GACNNNNNNGTC 1 cut(s) 194
EaeI YGGCCR 1 cut(s) 150
Eco147I AGGCCT 2 cut(s) 42, 59
Eco24I GRGCYC 1 cut(s) 35
Eco31I GGTCTC 1 cut(s) 834
Eco47I GGWCC 3 cut(s) 241, 853, 1024
Eco57I CTGAAG 1 cut(s) 49
EcoNI CCTNNNNNAGG 2 cut(s) 53, 64
EcoO109I RGGNCCY 1 cut(s) 32
EcoRII CCWGG 5 cut(s) 34, 54, 355, 365, 582
EcoT38I GRGCYC 1 cut(s) 35
FaeI CATG 3 cut(s) 151, 574, 795
FaqI GGGAC 2 cut(s) 641, 1013
FatI CATG 3 cut(s) 147, 570, 791
FauI CCCGC 2 cut(s) 96, 278
FblI GTMKAC 2 cut(s) 204, 785
Fnu4HI GCNGC 4 cut(s) 84, 255, 258, 380
FokI GGATG 3 cut(s) 62, 485, 614
FriOI GRGCYC 1 cut(s) 35
Fsp4HI GCNGC 4 cut(s) 84, 255, 258, 380
FspBI CTAG 5 cut(s) 176, 422, 555, 689, 821
GlaI GCGC 4 cut(s) 82, 107, 345, 347
GluI GCNGC 4 cut(s) 84, 255, 258, 380
GsuI CTGGAG 2 cut(s) 57, 746
HaeII RGCGCY 1 cut(s) 109
HaeIII GGCC 5 cut(s) 33, 42, 59, 70, 152
HapII CCGG 5 cut(s) 339, 437, 504, 674, 826
HgaI GACGC 3 cut(s) 121, 215, 649
HhaI GCGC 4 cut(s) 83, 108, 346, 348
Hin1I GRCGYC 2 cut(s) 132, 207
Hin1II CATG 3 cut(s) 151, 574, 795
Hin6I GCGC 4 cut(s) 81, 106, 344, 346
HinP1I GCGC 4 cut(s) 81, 106, 344, 346
HincII GTYRAC 2 cut(s) 205, 592
HindII GTYRAC 2 cut(s) 205, 592
HinfI GANTC 5 cut(s) 362, 541, 548, 733, 1001
HpaI GTTAAC 1 cut(s) 592
HpaII CCGG 5 cut(s) 339, 437, 504, 674, 826
HphI GGTGA 4 cut(s) 298, 572, 778, 914
Hpy166II GTNNAC 4 cut(s) 205, 592, 751, 786
Hpy188I TCNGA 6 cut(s) 314, 319, 513, 777, 839, 935
Hpy188III TCNNGA 8 cut(s) 331, 437, 478, 504, 545, 555, 599, 913
Hpy8I GTNNAC 4 cut(s) 205, 592, 751, 786
Hpy99I CGWCG 7 cut(s) 137, 146, 191, 206, 209, 314, 665
HpyAV CCTTC 6 cut(s) 70, 102, 254, 271, 844, 930
HpyCH4III ACNGT 3 cut(s) 310, 755, 890
HpyCH4IV ACGT 5 cut(s) 141, 226, 390, 486, 588
HpyCH4V TGCA 4 cut(s) 382, 697, 926, 967
HpyF10VI GCNNNNNNNGC 6 cut(s) 39, 144, 376, 602, 821, 928
HpyF3I CTNAG 5 cut(s) 512, 597, 634, 836, 995
HpySE526I ACGT 5 cut(s) 141, 226, 390, 486, 588
Hsp92I GRCGYC 2 cut(s) 132, 207
Hsp92II CATG 3 cut(s) 151, 574, 795
HspAI GCGC 4 cut(s) 81, 106, 344, 346
Kpn2I TCCGGA 2 cut(s) 436, 503
KspAI GTTAAC 1 cut(s) 592
Kzo9I GATC 2 cut(s) 314, 577
LmnI GCTCC 1 cut(s) 828
Lsp1109I GCAGC 4 cut(s) 95, 244, 266, 366
LweI GCATC 4 cut(s) 225, 592, 663, 684
MaeI CTAG 5 cut(s) 176, 422, 555, 689, 821
MaeII ACGT 5 cut(s) 141, 226, 390, 486, 588
MaeIII GTNAC 2 cut(s) 331, 955
MalI GATC 2 cut(s) 316, 579
MboI GATC 2 cut(s) 314, 577
MboII GAAGA 2 cut(s) 55, 742
MfeI CAATTG 1 cut(s) 798
MflI RGATCY 1 cut(s) 314
MhlI GDGCHC 2 cut(s) 35, 624
MlsI TGGCCA 1 cut(s) 152
MluCI AATT 1 cut(s) 798
MluNI TGGCCA 1 cut(s) 152
MlyI GAGTC 3 cut(s) 371, 542, 995
MmeI TCCRAC 3 cut(s) 292, 342, 880
Mox20I TGGCCA 1 cut(s) 152
MroI TCCGGA 2 cut(s) 436, 503
MroXI GAANNNNTTC 1 cut(s) 881
MscI TGGCCA 1 cut(s) 152
MseI TTAA 4 cut(s) 170, 426, 591, 1037
Msp20I TGGCCA 1 cut(s) 152
MspA1I CMGCKG 1 cut(s) 257
MspI CCGG 5 cut(s) 339, 437, 504, 674, 826
MspR9I CCNGG 6 cut(s) 36, 56, 357, 367, 584, 826
MunI CAATTG 1 cut(s) 798
MvaI CCWGG 5 cut(s) 36, 56, 357, 367, 584
MvnI CGCG 2 cut(s) 81, 346
MwoI GCNNNNNNNGC 6 cut(s) 39, 144, 376, 602, 821, 928
NciI CCSGG 1 cut(s) 826
NdeII GATC 2 cut(s) 314, 577
NlaIII CATG 3 cut(s) 151, 574, 795
NlaIV GGNNCC 3 cut(s) 33, 316, 566
NmeAIII GCCGAG 1 cut(s) 983
NmuCI GTSAC 2 cut(s) 331, 955
PauI GCGCGC 1 cut(s) 344
PceI AGGCCT 2 cut(s) 42, 59
PcsI WCGNNNNNNNCGW 1 cut(s) 306
PdmI GAANNNNTTC 1 cut(s) 881
PfeI GAWTC 2 cut(s) 541, 733
PinAI ACCGGT 1 cut(s) 338
PkrI GCNGC 4 cut(s) 85, 256, 259, 381
PleI GAGTC 3 cut(s) 370, 542, 995
PpsI GAGTC 3 cut(s) 370, 542, 995
Ppu21I YACGTR 2 cut(s) 227, 487
Psp6I CCWGG 5 cut(s) 34, 54, 355, 365, 582
PspGI CCWGG 5 cut(s) 34, 54, 355, 365, 582
PspN4I GGNNCC 3 cut(s) 33, 316, 566
PspOMI GGGCCC 1 cut(s) 31
PspPI GGNCC 6 cut(s) 31, 32, 69, 241, 853, 1024
PstI CTGCAG 1 cut(s) 384
PsuI RGATCY 1 cut(s) 314
PteI GCGCGC 1 cut(s) 344
PvuII CAGCTG 1 cut(s) 257
RsaI GTAC 2 cut(s) 431, 538
RsaNI GTAC 2 cut(s) 430, 537
SalI GTCGAC 1 cut(s) 203
SaqAI TTAA 4 cut(s) 170, 426, 591, 1037
SatI GCNGC 4 cut(s) 84, 255, 258, 380
Sau3AI GATC 2 cut(s) 314, 577
Sau96I GGNCC 6 cut(s) 31, 32, 69, 241, 853, 1024
ScaI AGTACT 1 cut(s) 431
SchI GAGTC 3 cut(s) 371, 542, 995
ScrFI CCNGG 6 cut(s) 36, 56, 357, 367, 584, 826
SduI GDGCHC 2 cut(s) 35, 624
SfaNI GCATC 4 cut(s) 225, 592, 663, 684
SfcI CTRYAG 1 cut(s) 380
SfiI GGCCNNNNNGGCC 1 cut(s) 39
SgrDI CGTCGACG 1 cut(s) 203
SinI GGWCC 3 cut(s) 241, 853, 1024
SmlI CTYRAG 1 cut(s) 913
SmoI CTYRAG 1 cut(s) 913
SpeI ACTAGT 1 cut(s) 421
Sse9I AATT 1 cut(s) 798
SseBI AGGCCT 2 cut(s) 42, 59
SsiI CCGC 4 cut(s) 79, 89, 271, 900
SspMI CTAG 5 cut(s) 176, 422, 555, 689, 821
StuI AGGCCT 2 cut(s) 42, 59
StyD4I CCNGG 6 cut(s) 34, 54, 355, 365, 582, 824
TaaI ACNGT 3 cut(s) 310, 755, 890
TaiI ACGT 5 cut(s) 144, 229, 393, 489, 591
TaqI TCGA 4 cut(s) 204, 533, 546, 576
TasI AATT 1 cut(s) 798
TatI WGTACW 1 cut(s) 429
TfiI GAWTC 2 cut(s) 541, 733
Tru1I TTAA 4 cut(s) 170, 426, 591, 1037
Tru9I TTAA 4 cut(s) 170, 426, 591, 1037
TscAI CASTG 5 cut(s) 474, 642, 729, 816, 873
TseFI GTSAC 2 cut(s) 331, 955
TseI GCWGC 4 cut(s) 83, 254, 257, 379
Tsp45I GTSAC 2 cut(s) 331, 955
TspDTI ATGAA 2 cut(s) 17, 780
TspGWI ACGGA 2 cut(s) 112, 280
TspRI CASTG 5 cut(s) 474, 642, 729, 816, 873
VpaK11BI GGWCC 3 cut(s) 241, 853, 1024
XagI CCTNNNNNAGG 2 cut(s) 53, 64
XbaI TCTAGA 1 cut(s) 554
XmiI GTMKAC 2 cut(s) 204, 785
XmnI GAANNNNTTC 1 cut(s) 881
XspI CTAG 5 cut(s) 176, 422, 555, 689, 821
ZrmI AGTACT 1 cut(s) 431
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.