pycom06g05170
ERF Family

Belongs to the LDH MDH superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0005141
Physical Location & Seq
Forward (+)
7135239 .. 7137085
1847 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g05170.2

Sequence Viewer

Length: 1053 bp
ATGCATAAAACTCCTTCAGGCTCCACGCTGGGCGCCGGTGGCCTCGACCTCACCCAGGCCTTCTTCAAGCCCATCTCCAATGCCGCCGCTCCCTCCCTCACCAAACGCCACACCAAGATCTCCGTCATCGGCGCCGGCAACGTCGGCATGGCCATCGCCCAGACCATCCTCACCCAAGACCTCGCCGACGAGCTCGTCCTCGTCGACGCCAAGCCCGACAAGCTCCGTGGCGAGATGCTCGACCTCCAGCACGCCGCCGCCTTCCTCCCCCGCACCAAGATCCTCGCCGACGTCGACTACGCCGTCACCGTCGGATCCGACCTCTGCATCGTCACCGCCGGCGCACGACAGATCGTTGGCGAGTCCAGGCTCAATCTCCTCCACCGCAACGTCGCTCTCTTTCGGAACATTATCCCGCCGCTAGCAATGTATTCGCCAGAGACGATCTTGATGATCGTGTCCAACCCGGTTGATATCTTGACTTACGTGGCCTGGAAGTTGTCCGGATTCCCCAGCAATCGGGTTGTCGGTACGGGTACCAATCTGGATTCGTCCCGGTTTCGGTTCCTCATCGCCGATCACCTCGATGTCAATGCTCAGGATGTGCAGGCTTACATTGTTGGGGAGCATGGTGACAGCTCGGTGGCATTGTGGTCGAGCATTAGTGTCGGGGGAGTGCCGGTGTTGAGTTTCTTAGAAAAGCAACAAATAGCATACGAGAAAGAGACGCTAGAGAGCATTCACAAGGCGGTTATAGACAGTGCCTATGAGGTGATAAGTCTGAAAGGGTATACTTCCTGGGCAATTGGGTACTCGGCAGCTGCGCTAGCTAAGAGCATACTGAGAGATCAGAGGAAGATTCACCCGGTTTCCGTGCTTGCAAAGGGGTTCTATGGGGTGGATGGTGGAGACGTGTTCTTGAGCTTGCCTGCCCAGCTTGGGAGGGGTGGGGTGTTGGGGGTTACCAACGTGCATTTGACTGATGAGGAGGAACAAAGGTTGAGAGATTCTGCTAATACCATTTTGGATTTGCAGGGTCAGTTGGGACTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko00270 ko00620 ko00640 ko01100 ko01110 ko01120 ko01130 ko04922 map00010 map00270 map00620 map00640 map01100 map01110 map01120 map01130 map04922
Pfam Domains
Protein Families
Ldh_1_C Ldh_1_N

Protein Analysis

351

Amino Acids

37.51

Weight (kDa)

5.99

Isoelectric Point (pI)

32.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 194, 302
AatII GACGTC 1 cut(s) 294
Acc65I GGTACC 1 cut(s) 536
AccB1I GGYRCC 3 cut(s) 32, 131, 536
AccBSI CCGCTC 1 cut(s) 89
AccI GTMKAC 3 cut(s) 204, 294, 791
AccIII TCCGGA 1 cut(s) 503
AclWI GGATC 3 cut(s) 274, 309, 322
AcoI YGGCCR 1 cut(s) 150
AcyI GRCGYC 4 cut(s) 33, 132, 207, 291
AfaI GTAC 3 cut(s) 532, 538, 812
AfiI CCNNNNNNNGG 4 cut(s) 55, 519, 561, 939
AflIII ACRYGT 1 cut(s) 912
AgsI TTSAA 1 cut(s) 67
AjiI CACGTC 1 cut(s) 913
AjnI CCWGG 4 cut(s) 54, 365, 491, 797
AluBI AGCT 7 cut(s) 193, 223, 639, 821, 830, 924, 937
AluI AGCT 7 cut(s) 193, 223, 639, 821, 830, 924, 937
Alw21I GWGCWC 1 cut(s) 195
Alw26I GTCTC 3 cut(s) 434, 719, 903
AlwI GGATC 3 cut(s) 274, 309, 322
Aor13HI TCCGGA 1 cut(s) 503
AoxI GGCC 4 cut(s) 40, 57, 150, 489
ApeKI GCWGC 2 cut(s) 818, 821
Asp718I GGTACC 1 cut(s) 536
AspLEI GCGC 4 cut(s) 35, 134, 344, 826
AsuC2I CCSGG 3 cut(s) 467, 556, 866
AsuHPI GGTGA 9 cut(s) 43, 91, 163, 298, 325, 572, 644, 784, 854
AsuNHI GCTAGC 2 cut(s) 421, 826
BalI TGGCCA 1 cut(s) 152
BamHI GGATCC 1 cut(s) 314
BanI GGYRCC 3 cut(s) 32, 131, 536
BanII GRGCYC 1 cut(s) 195
Bbv12I GWGCWC 1 cut(s) 195
BbvI GCAGC 2 cut(s) 808, 830
BccI CCATC 4 cut(s) 80, 161, 173, 896
BceAI ACGGC 1 cut(s) 287
BciT130I CCWGG 4 cut(s) 56, 367, 493, 799
BcnI CCSGG 3 cut(s) 467, 556, 866
BcoDI GTCTC 3 cut(s) 434, 719, 903
BfaI CTAG 3 cut(s) 422, 731, 827
BfoI RGCGCY 2 cut(s) 36, 135
BglII AGATCT 1 cut(s) 117
BisI GCNGC 7 cut(s) 84, 87, 255, 258, 419, 819, 822
BlsI GCNGC 7 cut(s) 85, 88, 256, 259, 420, 820, 823
Bme1390I CCNGG 7 cut(s) 56, 367, 467, 493, 556, 799, 866
BmgBI CACGTC 1 cut(s) 913
BmiI GGNNCC 6 cut(s) 22, 34, 133, 316, 538, 566
BmrFI CCNGG 7 cut(s) 56, 367, 467, 493, 556, 799, 866
BmsI GCATC 2 cut(s) 225, 336
BmtI GCTAGC 2 cut(s) 425, 830
BpmI CTGGAG 1 cut(s) 230
Bpu10I CCTNAGC 1 cut(s) 597
BpuEI CTTGAG 1 cut(s) 940
BpuMI CCSGG 3 cut(s) 467, 556, 866
BsaAI YACGTR 1 cut(s) 487
BsaHI GRCGYC 4 cut(s) 33, 132, 207, 291
BsaJI CCNNGG 3 cut(s) 54, 226, 798
BsaWI WCCGGW 1 cut(s) 503
BsaXI ACNNNNNCTCC 2 cut(s) 666, 696
Bsc4I CCNNNNNNNGG 4 cut(s) 55, 519, 561, 939
Bse118I RCCGGY 4 cut(s) 35, 134, 338, 679
Bse3DI GCAATG 1 cut(s) 432
BseAI TCCGGA 1 cut(s) 503
BseBI CCWGG 4 cut(s) 56, 367, 493, 799
BseDI CCNNGG 3 cut(s) 54, 226, 798
BseGI GGATG 3 cut(s) 165, 607, 907
BseLI CCNNNNNNNGG 4 cut(s) 55, 519, 561, 939
BseMI GCAATG 1 cut(s) 432
BseMII CTCAG 2 cut(s) 611, 833
BseRI GAGGAG 2 cut(s) 368, 1001
BseXI GCAGC 2 cut(s) 808, 830
BseYI CCCAGC 3 cut(s) 28, 512, 933
BsgI GTGCAG 1 cut(s) 626
BshFI GGCC 4 cut(s) 42, 59, 152, 491
BshNI GGYRCC 3 cut(s) 32, 131, 536
BsiHKAI GWGCWC 1 cut(s) 195
BsiSI CCGG 8 cut(s) 36, 135, 339, 467, 504, 556, 680, 866
BslFI GGGAC 1 cut(s) 538
BslI CCNNNNNNNGG 4 cut(s) 55, 519, 561, 939
BsmAI GTCTC 3 cut(s) 434, 719, 903
BsmBI CGTCTC 3 cut(s) 434, 719, 903
BsmFI GGGAC 1 cut(s) 538
BsmI GAATGC 1 cut(s) 738
BsnI GGCC 4 cut(s) 42, 59, 152, 491
Bsp1286I GDGCHC 1 cut(s) 195
Bsp13I TCCGGA 1 cut(s) 503
Bsp143I GATC 8 cut(s) 117, 279, 314, 351, 444, 453, 577, 847
BspANI GGCC 4 cut(s) 42, 59, 152, 491
BspCNI CTCAG 2 cut(s) 610, 834
BspEI TCCGGA 1 cut(s) 503
BspLI GGNNCC 6 cut(s) 22, 34, 133, 316, 538, 566
BspOI GCTAGC 2 cut(s) 425, 830
BspPI GGATC 3 cut(s) 274, 309, 322
BspT107I GGYRCC 3 cut(s) 32, 131, 536
BsrBI CCGCTC 1 cut(s) 89
BsrDI GCAATG 1 cut(s) 432
BsrFI RCCGGY 4 cut(s) 35, 134, 338, 679
BssAI RCCGGY 4 cut(s) 35, 134, 338, 679
BssECI CCNNGG 3 cut(s) 54, 226, 798
BssMI GATC 8 cut(s) 117, 279, 314, 351, 444, 453, 577, 847
BssNAI GTATAC 1 cut(s) 792
BssNI GRCGYC 4 cut(s) 33, 132, 207, 291
Bst1107I GTATAC 1 cut(s) 792
Bst2UI CCWGG 4 cut(s) 56, 367, 493, 799
Bst4CI ACNGT 2 cut(s) 310, 761
BstACI GRCGYC 4 cut(s) 33, 132, 207, 291
BstBAI YACGTR 1 cut(s) 487
BstC8I GCNNGC 9 cut(s) 136, 252, 340, 423, 609, 828, 879, 926, 930
BstDEI CTNAG 4 cut(s) 597, 694, 831, 842
BstDSI CCRYGG 1 cut(s) 226
BstEII GGTNACC 1 cut(s) 961
BstENI CCTNNNNNAGG 1 cut(s) 53
BstF5I GGATG 3 cut(s) 165, 607, 907
BstH2I RGCGCY 2 cut(s) 36, 135
BstHHI GCGC 4 cut(s) 35, 134, 344, 826
BstKTI GATC 8 cut(s) 120, 282, 317, 354, 447, 456, 580, 850
BstMAI GTCTC 3 cut(s) 434, 719, 903
BstMBI GATC 8 cut(s) 117, 279, 314, 351, 444, 453, 577, 847
BstMWI GCNNNNNNNGC 5 cut(s) 39, 144, 220, 827, 934
BstNI CCWGG 4 cut(s) 56, 367, 493, 799
BstPI GGTNACC 1 cut(s) 961
BstSCI CCNGG 7 cut(s) 54, 365, 465, 491, 554, 797, 864
BstV1I GCAGC 2 cut(s) 808, 830
BstX2I RGATCY 3 cut(s) 117, 279, 314
BstYI RGATCY 3 cut(s) 117, 279, 314
BstZ17I GTATAC 1 cut(s) 792
BsuRI GGCC 4 cut(s) 42, 59, 152, 491
BtgI CCRYGG 1 cut(s) 226
BtgZI GCGATG 2 cut(s) 139, 556
BtrI CACGTC 1 cut(s) 913
BtsCI GGATG 3 cut(s) 165, 607, 907
BtsIMutI CAGTG 1 cut(s) 766
Cac8I GCNNGC 9 cut(s) 136, 252, 340, 423, 609, 828, 879, 926, 930
CfoI GCGC 4 cut(s) 35, 134, 344, 826
Cfr10I RCCGGY 4 cut(s) 35, 134, 338, 679
CseI GACGC 2 cut(s) 215, 736
Csp6I GTAC 3 cut(s) 531, 537, 811
CspCI CAANNNNNGTGG 2 cut(s) 208, 243
CviAII CATG 2 cut(s) 148, 629
CviQI GTAC 3 cut(s) 531, 537, 811
DdeI CTNAG 4 cut(s) 597, 694, 831, 842
DinI GGCGCC 2 cut(s) 34, 133
DpnI GATC 8 cut(s) 119, 281, 316, 353, 446, 455, 579, 849
DpnII GATC 8 cut(s) 117, 279, 314, 351, 444, 453, 577, 847
DrdI GACNNNNNNGTC 2 cut(s) 194, 302
DseDI GACNNNNNNGTC 2 cut(s) 194, 302
EaeI YGGCCR 1 cut(s) 150
Ecl136II GAGCTC 1 cut(s) 193
Eco147I AGGCCT 1 cut(s) 59
Eco24I GRGCYC 1 cut(s) 195
Eco32I GATATC 1 cut(s) 475
Eco53kI GAGCTC 1 cut(s) 193
Eco91I GGTNACC 1 cut(s) 961
EcoICRI GAGCTC 1 cut(s) 193
EcoNI CCTNNNNNAGG 1 cut(s) 53
EcoO65I GGTNACC 1 cut(s) 961
EcoRII CCWGG 4 cut(s) 54, 365, 491, 797
EcoRV GATATC 1 cut(s) 475
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 195
EgeI GGCGCC 2 cut(s) 34, 133
EheI GGCGCC 2 cut(s) 34, 133
Esp3I CGTCTC 3 cut(s) 434, 719, 903
FaeI CATG 2 cut(s) 151, 632
FaiI YATR 9 cut(s) 6, 149, 630, 715, 755, 768, 792, 839, 894
FaqI GGGAC 1 cut(s) 538
FatI CATG 2 cut(s) 147, 628
FauI CCCGC 2 cut(s) 278, 423
FblI GTMKAC 3 cut(s) 204, 294, 791
Fnu4HI GCNGC 7 cut(s) 84, 87, 255, 258, 419, 819, 822
FokI GGATG 3 cut(s) 152, 614, 914
FriOI GRGCYC 1 cut(s) 195
Fsp4HI GCNGC 7 cut(s) 84, 87, 255, 258, 419, 819, 822
FspBI CTAG 3 cut(s) 422, 731, 827
GlaI GCGC 4 cut(s) 34, 133, 343, 825
GluI GCNGC 7 cut(s) 84, 87, 255, 258, 419, 819, 822
GsaI CCCAGC 3 cut(s) 32, 516, 937
GsuI CTGGAG 1 cut(s) 230
HaeII RGCGCY 2 cut(s) 36, 135
HaeIII GGCC 4 cut(s) 42, 59, 152, 491
HapII CCGG 8 cut(s) 36, 135, 339, 467, 504, 556, 680, 866
HgaI GACGC 2 cut(s) 215, 736
HhaI GCGC 4 cut(s) 35, 134, 344, 826
Hin1I GRCGYC 4 cut(s) 33, 132, 207, 291
Hin1II CATG 2 cut(s) 151, 632
Hin6I GCGC 4 cut(s) 33, 132, 342, 824
HinP1I GCGC 4 cut(s) 33, 132, 342, 824
HincII GTYRAC 2 cut(s) 205, 295
HindII GTYRAC 2 cut(s) 205, 295
HinfI GANTC 6 cut(s) 362, 507, 548, 859, 1007, 1047
HpaII CCGG 8 cut(s) 36, 135, 339, 467, 504, 556, 680, 866
HphI GGTGA 9 cut(s) 43, 91, 163, 298, 325, 572, 644, 784, 854
Hpy166II GTNNAC 3 cut(s) 205, 295, 792
Hpy188I TCNGA 6 cut(s) 314, 319, 405, 783, 852, 1052
Hpy188III TCNNGA 6 cut(s) 448, 478, 504, 545, 599, 919
Hpy8I GTNNAC 3 cut(s) 205, 295, 792
Hpy99I CGWCG 8 cut(s) 146, 191, 206, 209, 293, 296, 314, 395
HpyAV CCTTC 3 cut(s) 24, 70, 271
HpyCH4III ACNGT 2 cut(s) 310, 761
HpyCH4IV ACGT 6 cut(s) 141, 291, 390, 486, 912, 969
HpyCH4V TGCA 6 cut(s) 4, 327, 607, 881, 973, 1033
HpyF10VI GCNNNNNNNGC 5 cut(s) 39, 144, 220, 827, 934
HpyF3I CTNAG 4 cut(s) 597, 694, 831, 842
HpySE526I ACGT 6 cut(s) 141, 291, 390, 486, 912, 969
Hsp92I GRCGYC 4 cut(s) 33, 132, 207, 291
Hsp92II CATG 2 cut(s) 151, 632
HspAI GCGC 4 cut(s) 33, 132, 342, 824
KasI GGCGCC 2 cut(s) 32, 131
Kpn2I TCCGGA 1 cut(s) 503
KpnI GGTACC 1 cut(s) 540
KroI GCCGGC 2 cut(s) 134, 338
KroNI GCCGGC 2 cut(s) 136, 340
Kzo9I GATC 8 cut(s) 117, 279, 314, 351, 444, 453, 577, 847
LmnI GCTCC 4 cut(s) 26, 94, 228, 625
Lsp1109I GCAGC 2 cut(s) 808, 830
LweI GCATC 2 cut(s) 225, 336
MaeI CTAG 3 cut(s) 422, 731, 827
MaeII ACGT 6 cut(s) 141, 291, 390, 486, 912, 969
MaeIII GTNAC 4 cut(s) 304, 331, 632, 961
MalI GATC 8 cut(s) 119, 281, 316, 353, 446, 455, 579, 849
MbiI CCGCTC 1 cut(s) 89
MboI GATC 8 cut(s) 117, 279, 314, 351, 444, 453, 577, 847
MboII GAAGA 2 cut(s) 55, 868
MfeI CAATTG 1 cut(s) 804
MflI RGATCY 3 cut(s) 117, 279, 314
MhlI GDGCHC 1 cut(s) 195
MlsI TGGCCA 1 cut(s) 152
MluCI AATT 1 cut(s) 804
MluNI TGGCCA 1 cut(s) 152
Mly113I GGCGCC 2 cut(s) 33, 132
MlyI GAGTC 2 cut(s) 371, 1041
MmeI TCCRAC 3 cut(s) 292, 342, 486
Mox20I TGGCCA 1 cut(s) 152
Mph1103I ATGCAT 1 cut(s) 6
MreI CGCCGGCG 1 cut(s) 338
MroI TCCGGA 1 cut(s) 503
MroNI GCCGGC 2 cut(s) 134, 338
MscI TGGCCA 1 cut(s) 152
MslI CAYNNNNRTG 1 cut(s) 585
Msp20I TGGCCA 1 cut(s) 152
MspA1I CMGCKG 1 cut(s) 821
MspI CCGG 8 cut(s) 36, 135, 339, 467, 504, 556, 680, 866
MspR9I CCNGG 7 cut(s) 56, 367, 467, 493, 556, 799, 866
MunI CAATTG 1 cut(s) 804
Mva1269I GAATGC 1 cut(s) 738
MvaI CCWGG 4 cut(s) 56, 367, 493, 799
MwoI GCNNNNNNNGC 5 cut(s) 39, 144, 220, 827, 934
NaeI GCCGGC 2 cut(s) 136, 340
NarI GGCGCC 2 cut(s) 33, 132
NciI CCSGG 3 cut(s) 467, 556, 866
NdeII GATC 8 cut(s) 117, 279, 314, 351, 444, 453, 577, 847
NgoMIV GCCGGC 2 cut(s) 134, 338
NheI GCTAGC 2 cut(s) 421, 826
NlaIII CATG 2 cut(s) 151, 632
NlaIV GGNNCC 6 cut(s) 22, 34, 133, 316, 538, 566
NmeAIII GCCGAG 1 cut(s) 794
NmuCI GTSAC 3 cut(s) 304, 331, 632
NsiI ATGCAT 1 cut(s) 6
PceI AGGCCT 1 cut(s) 59
PcsI WCGNNNNNNNCGW 6 cut(s) 201, 213, 291, 300, 306, 440
PctI GAATGC 1 cut(s) 738
PdiI GCCGGC 2 cut(s) 136, 340
PfeI GAWTC 4 cut(s) 507, 548, 859, 1007
PkrI GCNGC 7 cut(s) 85, 88, 256, 259, 420, 820, 823
PleI GAGTC 2 cut(s) 370, 1041
PluTI GGCGCC 2 cut(s) 36, 135
PpsI GAGTC 2 cut(s) 370, 1041
Ppu21I YACGTR 1 cut(s) 487
Psp124BI GAGCTC 1 cut(s) 195
Psp6I CCWGG 4 cut(s) 54, 365, 491, 797
PspEI GGTNACC 1 cut(s) 961
PspFI CCCAGC 3 cut(s) 28, 512, 933
PspGI CCWGG 4 cut(s) 54, 365, 491, 797
PspN4I GGNNCC 6 cut(s) 22, 34, 133, 316, 538, 566
PsuI RGATCY 3 cut(s) 117, 279, 314
PvuII CAGCTG 1 cut(s) 821
RsaI GTAC 3 cut(s) 532, 538, 812
RsaNI GTAC 3 cut(s) 531, 537, 811
RseI CAYNNNNRTG 1 cut(s) 585
SacI GAGCTC 1 cut(s) 195
SalI GTCGAC 2 cut(s) 203, 293
SatI GCNGC 7 cut(s) 84, 87, 255, 258, 419, 819, 822
Sau3AI GATC 8 cut(s) 117, 279, 314, 351, 444, 453, 577, 847
SchI GAGTC 2 cut(s) 371, 1041
ScrFI CCNGG 7 cut(s) 56, 367, 467, 493, 556, 799, 866
SduI GDGCHC 1 cut(s) 195
SfaNI GCATC 2 cut(s) 225, 336
SfoI GGCGCC 2 cut(s) 34, 133
SgrAI CRCCGGYG 2 cut(s) 35, 338
SgrDI CGTCGACG 1 cut(s) 203
SmiMI CAYNNNNRTG 1 cut(s) 585
SmlI CTYRAG 1 cut(s) 919
SmoI CTYRAG 1 cut(s) 919
Sse9I AATT 1 cut(s) 804
SseBI AGGCCT 1 cut(s) 59
SspDI GGCGCC 2 cut(s) 32, 131
SspMI CTAG 3 cut(s) 422, 731, 827
SstI GAGCTC 1 cut(s) 195
StuI AGGCCT 1 cut(s) 59
StyD4I CCNGG 7 cut(s) 54, 365, 465, 491, 554, 797, 864
TaaI ACNGT 2 cut(s) 310, 761
TaiI ACGT 6 cut(s) 144, 294, 393, 489, 915, 972
TaqI TCGA 6 cut(s) 45, 204, 240, 294, 585, 656
TasI AATT 1 cut(s) 804
TauI GCSGC 5 cut(s) 86, 89, 257, 260, 421
TfiI GAWTC 4 cut(s) 507, 548, 859, 1007
TscAI CASTG 1 cut(s) 766
TseFI GTSAC 3 cut(s) 304, 331, 632
TseI GCWGC 2 cut(s) 818, 821
Tsp45I GTSAC 3 cut(s) 304, 331, 632
TspGWI ACGGA 3 cut(s) 112, 215, 862
TspRI CASTG 1 cut(s) 766
XagI CCTNNNNNAGG 1 cut(s) 53
XmiI GTMKAC 3 cut(s) 204, 294, 791
XspI CTAG 3 cut(s) 422, 731, 827
ZraI GACGTC 1 cut(s) 292
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.