Rh4DG432100
ERF Family

Belongs to the LDH MDH superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0005141
Physical Location & Seq
Forward (+)
63629148 .. 63630740
1593 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG432100.1

Sequence Viewer

Length: 1059 bp
ATGAACAAAAGCAGTTCGGCTTCGTCATTGGGCCCTGGAGGCCTTGACCTAACCCAGGCCTTCTTCAGGCCCATCAGCCGCGCAGCCCCGCCTTCCCCGACCAAGCGCCACAACAAAATCTCCGTCATTGGCGTCGGCAACGTCGGCATGGCCATAGCACAAACCATCTTAACCCAAGACCTCGCCGACGAGCTTGTCCTCGTCGACGCCAAACCTGAAAAGCTACGTGGAGAGATGCTGGACCTTCAACACGCAGCTGCCTTCTTACCTCGCACCAAAATCCACGCCTCCGTGGACTACGCCATCACCGTCGGATCCGACCTCTGCATCGTGACGGCCGGTGCGCGCCAGAACCCTGGCGAGTCCAGGCTGAACCTGCTGCAGAGGAACGTGGCTCTCTTTAGAAATATCATACCTCCACTAGCTAAGTACTCTCCGGATACCATATTACTCATCGTGTCCAATCCAGTGGATGTCCTGACTTACGTGGCGTGGAAGCTATCCGGATTTCCGAGCAACAGGGTTTTCGGGTCGGGTACGAATCTCGACTCATCTAGGTTTCGGTTCCTCATGGCCGATCACCTGGACGTTAACGCTCAGGATGTGCAGGCTTACATCATTGGCGAGCATGGGGACAGCTCAGTGGCTTTGTGGTCAAGCATTAGCGTCGGGGGAGTGCCGGTGTTGAGCTTCCTAGAGAAGCAGCAAATAGCATACGAGAAAGAGACACTGGAGAAGATTCACAAAGCAGTTGTGGACTGTGCGTATGAGGTGATAAGTCTGAAAGGGTATACTTCATGGGCAATTGGTTACTCAGTGGCAAACCTAGCCCGGAGCATACTCAGAGACCAGAGGAAGGTCCAACCTGTTTCAGTGCTTGCCAATGGGTTCTACGGTGTTGGTGGAGGTGATGTGTTCTTGAGCTTGCCTGCACAGCTCGGAAGGAGTGGAGTTCTGGGAGTGACCAACGTGCATTTGACTGATGAGGAGGCACAGAAGCTAAGGGACTCGGCTCACACCATTTTGGAGGTCCAAAGTCAGTTAAACTTGGGAATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko00270 ko00620 ko00640 ko01100 ko01110 ko01120 ko01130 ko04922 map00010 map00270 map00620 map00640 map01100 map01110 map01120 map01130 map04922
Pfam Domains
Protein Families
Ldh_1_C Ldh_1_N

Protein Analysis

352

Amino Acids

37.91

Weight (kDa)

7.1

Isoelectric Point (pI)

39.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ldh_1_N PF00056 39 - 177 2.1e-44 lactate/malate dehydrogenase, NAD binding domain
Ldh_1_C PF02866 180 - 343 1.8e-32 lactate/malate dehydrogenase, alpha/beta C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 194
Acc36I ACCTGC 1 cut(s) 384
AccI GTMKAC 2 cut(s) 204, 791
AccII CGCG 2 cut(s) 81, 346
AccIII TCCGGA 2 cut(s) 436, 503
AciI CCGC 2 cut(s) 79, 89
AclWI GGATC 2 cut(s) 309, 322
AcoI YGGCCR 3 cut(s) 150, 336, 573
AcuI CTGAAG 1 cut(s) 49
AcyI GRCGYC 2 cut(s) 132, 207
AfaI GTAC 2 cut(s) 431, 538
AfiI CCNNNNNNNGG 3 cut(s) 55, 66, 856
AgsI TTSAA 1 cut(s) 248
AjnI CCWGG 5 cut(s) 34, 54, 355, 365, 582
Alw26I GTCTC 2 cut(s) 719, 840
AlwI GGATC 2 cut(s) 309, 322
Aor13HI TCCGGA 2 cut(s) 436, 503
AoxI GGCC 7 cut(s) 31, 40, 57, 68, 150, 336, 573
ApaI GGGCCC 1 cut(s) 35
ApeKI GCWGC 5 cut(s) 83, 254, 257, 379, 703
ArsI GACNNNNNNTTYG 2 cut(s) 108, 140
AspLEI GCGC 4 cut(s) 83, 108, 346, 348
AspS9I GGNCC 6 cut(s) 31, 32, 69, 241, 859, 1030
AsuC2I CCSGG 1 cut(s) 832
AsuHPI GGTGA 4 cut(s) 298, 572, 784, 920
AvaII GGWCC 3 cut(s) 241, 859, 1030
BaeGI GKGCMC 1 cut(s) 35
BalI TGGCCA 1 cut(s) 152
BamHI GGATCC 1 cut(s) 314
BanII GRGCYC 1 cut(s) 35
BbvI GCAGC 5 cut(s) 95, 244, 266, 366, 715
BccI CCATC 3 cut(s) 80, 173, 311
BceAI ACGGC 1 cut(s) 351
BcgI CGANNNNNNTGC 2 cut(s) 649, 683
BciT130I CCWGG 5 cut(s) 36, 56, 357, 367, 584
BciVI GTATCC 1 cut(s) 433
BcnI CCSGG 1 cut(s) 832
BcoDI GTCTC 2 cut(s) 719, 840
BfaI CTAG 4 cut(s) 422, 555, 695, 827
BfmI CTRYAG 1 cut(s) 380
BfoI RGCGCY 1 cut(s) 109
BfuAI ACCTGC 1 cut(s) 384
BfuI GTATCC 1 cut(s) 433
BglI GCCNNNNNGGC 1 cut(s) 39
BisI GCNGC 6 cut(s) 79, 84, 255, 258, 380, 704
BlsI GCNGC 6 cut(s) 80, 85, 256, 259, 381, 705
BmcAI AGTACT 1 cut(s) 431
Bme1390I CCNGG 6 cut(s) 36, 56, 357, 367, 584, 832
Bme18I GGWCC 3 cut(s) 241, 859, 1030
BmgT120I GGNCC 6 cut(s) 31, 32, 69, 241, 859, 1030
BmiI GGNNCC 3 cut(s) 33, 316, 566
BmrFI CCNGG 6 cut(s) 36, 56, 357, 367, 584, 832
BmsI GCATC 2 cut(s) 225, 336
BpmI CTGGAG 2 cut(s) 57, 752
Bpu10I CCTNAGC 2 cut(s) 597, 1001
BpuEI CTTGAG 1 cut(s) 940
BpuMI CCSGG 1 cut(s) 832
BsaAI YACGTR 2 cut(s) 227, 487
BsaHI GRCGYC 2 cut(s) 132, 207
BsaI GGTCTC 1 cut(s) 840
BsaJI CCNNGG 4 cut(s) 34, 54, 291, 355
BsaWI WCCGGW 2 cut(s) 436, 503
BsaXI ACNNNNNCTCC 6 cut(s) 104, 134, 666, 696, 897, 927
Bsc4I CCNNNNNNNGG 3 cut(s) 55, 66, 856
Bse118I RCCGGY 2 cut(s) 338, 679
Bse1I ACTGG 2 cut(s) 467, 735
BseAI TCCGGA 2 cut(s) 436, 503
BseBI CCWGG 5 cut(s) 36, 56, 357, 367, 584
BseDI CCNNGG 4 cut(s) 34, 54, 291, 355
BseGI GGATG 2 cut(s) 478, 607
BseLI CCNNNNNNNGG 3 cut(s) 55, 66, 856
BseMII CTCAG 4 cut(s) 611, 654, 828, 856
BseNI ACTGG 2 cut(s) 467, 735
BsePI GCGCGC 1 cut(s) 344
BseRI GAGGAG 1 cut(s) 1001
BseSI GKGCMC 1 cut(s) 35
BseX3I CGGCCG 1 cut(s) 336
BseXI GCAGC 5 cut(s) 95, 244, 266, 366, 715
BsgI GTGCAG 2 cut(s) 626, 915
Bsh1236I CGCG 2 cut(s) 81, 346
Bsh1285I CGRYCG 1 cut(s) 339
BshFI GGCC 7 cut(s) 33, 42, 59, 70, 152, 338, 575
BsiEI CGRYCG 1 cut(s) 339
BsiSI CCGG 5 cut(s) 339, 437, 504, 680, 832
BslFI GGGAC 2 cut(s) 647, 1019
BslI CCNNNNNNNGG 3 cut(s) 55, 66, 856
BsmAI GTCTC 2 cut(s) 719, 840
BsmFI GGGAC 2 cut(s) 647, 1019
BsnI GGCC 7 cut(s) 33, 42, 59, 70, 152, 338, 575
Bso31I GGTCTC 1 cut(s) 840
Bsp120I GGGCCC 1 cut(s) 31
Bsp1286I GDGCHC 1 cut(s) 35
Bsp13I TCCGGA 2 cut(s) 436, 503
Bsp143I GATC 2 cut(s) 314, 577
BspACI CCGC 2 cut(s) 79, 89
BspANI GGCC 7 cut(s) 33, 42, 59, 70, 152, 338, 575
BspCNI CTCAG 4 cut(s) 610, 653, 827, 855
BspEI TCCGGA 2 cut(s) 436, 503
BspFNI CGCG 2 cut(s) 81, 346
BspLI GGNNCC 3 cut(s) 33, 316, 566
BspMAI CTGCAG 1 cut(s) 384
BspMI ACCTGC 1 cut(s) 384
BspPI GGATC 2 cut(s) 309, 322
BspTNI GGTCTC 1 cut(s) 840
BsrFI RCCGGY 2 cut(s) 338, 679
BsrI ACTGG 2 cut(s) 467, 735
BssAI RCCGGY 2 cut(s) 338, 679
BssECI CCNNGG 4 cut(s) 34, 54, 291, 355
BssHII GCGCGC 1 cut(s) 344
BssMI GATC 2 cut(s) 314, 577
BssNAI GTATAC 1 cut(s) 792
BssNI GRCGYC 2 cut(s) 132, 207
Bst1107I GTATAC 1 cut(s) 792
Bst2UI CCWGG 5 cut(s) 36, 56, 357, 367, 584
Bst4CI ACNGT 3 cut(s) 310, 761, 896
BstACI GRCGYC 2 cut(s) 132, 207
BstBAI YACGTR 2 cut(s) 227, 487
BstC8I GCNNGC 6 cut(s) 346, 609, 626, 879, 926, 930
BstDEI CTNAG 6 cut(s) 426, 597, 640, 814, 842, 1001
BstDSI CCRYGG 1 cut(s) 291
BstENI CCTNNNNNAGG 2 cut(s) 53, 64
BstF5I GGATG 2 cut(s) 478, 607
BstFNI CGCG 2 cut(s) 81, 346
BstH2I RGCGCY 1 cut(s) 109
BstHHI GCGC 4 cut(s) 83, 108, 346, 348
BstKTI GATC 2 cut(s) 317, 580
BstMAI GTCTC 2 cut(s) 719, 840
BstMBI GATC 2 cut(s) 314, 577
BstMCI CGRYCG 1 cut(s) 339
BstMWI GCNNNNNNNGC 5 cut(s) 39, 144, 376, 827, 934
BstNI CCWGG 5 cut(s) 36, 56, 357, 367, 584
BstSCI CCNGG 6 cut(s) 34, 54, 355, 365, 582, 830
BstSFI CTRYAG 1 cut(s) 380
BstSLI GKGCMC 1 cut(s) 35
BstUI CGCG 2 cut(s) 81, 346
BstV1I GCAGC 5 cut(s) 95, 244, 266, 366, 715
BstX2I RGATCY 1 cut(s) 314
BstXI CCANNNNNNTGG 2 cut(s) 356, 469
BstYI RGATCY 1 cut(s) 314
BstZ17I GTATAC 1 cut(s) 792
BstZI CGGCCG 1 cut(s) 336
BsuI GTATCC 1 cut(s) 433
BsuRI GGCC 7 cut(s) 33, 42, 59, 70, 152, 338, 575
BtgI CCRYGG 1 cut(s) 291
BtsCI GGATG 2 cut(s) 478, 607
BtsIMutI CAGTG 5 cut(s) 474, 648, 728, 822, 879
BveI ACCTGC 1 cut(s) 384
Cac8I GCNNGC 6 cut(s) 346, 609, 626, 879, 926, 930
CfoI GCGC 4 cut(s) 83, 108, 346, 348
Cfr10I RCCGGY 2 cut(s) 338, 679
Cfr13I GGNCC 6 cut(s) 31, 32, 69, 241, 859, 1030
CseI GACGC 3 cut(s) 121, 215, 655
Csp6I GTAC 2 cut(s) 430, 537
CviAII CATG 4 cut(s) 148, 571, 629, 798
CviQI GTAC 2 cut(s) 430, 537
DdeI CTNAG 6 cut(s) 426, 597, 640, 814, 842, 1001
DpnI GATC 2 cut(s) 316, 579
DpnII GATC 2 cut(s) 314, 577
DrdI GACNNNNNNGTC 1 cut(s) 194
DseDI GACNNNNNNGTC 1 cut(s) 194
EaeI YGGCCR 3 cut(s) 150, 336, 573
EagI CGGCCG 1 cut(s) 336
EclXI CGGCCG 1 cut(s) 336
Eco147I AGGCCT 2 cut(s) 42, 59
Eco24I GRGCYC 1 cut(s) 35
Eco31I GGTCTC 1 cut(s) 840
Eco47I GGWCC 3 cut(s) 241, 859, 1030
Eco52I CGGCCG 1 cut(s) 336
Eco57I CTGAAG 1 cut(s) 49
EcoNI CCTNNNNNAGG 2 cut(s) 53, 64
EcoO109I RGGNCCY 1 cut(s) 32
EcoRII CCWGG 5 cut(s) 34, 54, 355, 365, 582
EcoT38I GRGCYC 1 cut(s) 35
FaeI CATG 4 cut(s) 151, 574, 632, 801
FaqI GGGAC 2 cut(s) 647, 1019
FatI CATG 4 cut(s) 147, 570, 628, 797
FauI CCCGC 1 cut(s) 96
FblI GTMKAC 2 cut(s) 204, 791
Fnu4HI GCNGC 6 cut(s) 79, 84, 255, 258, 380, 704
FokI GGATG 2 cut(s) 485, 614
FriOI GRGCYC 1 cut(s) 35
Fsp4HI GCNGC 6 cut(s) 79, 84, 255, 258, 380, 704
FspBI CTAG 4 cut(s) 422, 555, 695, 827
GlaI GCGC 4 cut(s) 82, 107, 345, 347
GluI GCNGC 6 cut(s) 79, 84, 255, 258, 380, 704
GsuI CTGGAG 2 cut(s) 57, 752
HaeII RGCGCY 1 cut(s) 109
HaeIII GGCC 7 cut(s) 33, 42, 59, 70, 152, 338, 575
HapII CCGG 5 cut(s) 339, 437, 504, 680, 832
HgaI GACGC 3 cut(s) 121, 215, 655
HhaI GCGC 4 cut(s) 83, 108, 346, 348
Hin1I GRCGYC 2 cut(s) 132, 207
Hin1II CATG 4 cut(s) 151, 574, 632, 801
Hin6I GCGC 4 cut(s) 81, 106, 344, 346
HinP1I GCGC 4 cut(s) 81, 106, 344, 346
HincII GTYRAC 2 cut(s) 205, 592
HindII GTYRAC 2 cut(s) 205, 592
HinfI GANTC 5 cut(s) 362, 541, 548, 739, 1007
HpaI GTTAAC 1 cut(s) 592
HpaII CCGG 5 cut(s) 339, 437, 504, 680, 832
HphI GGTGA 4 cut(s) 298, 572, 784, 920
Hpy166II GTNNAC 5 cut(s) 205, 295, 592, 757, 792
Hpy188I TCNGA 6 cut(s) 314, 319, 513, 783, 845, 941
Hpy188III TCNNGA 7 cut(s) 331, 437, 478, 504, 545, 599, 919
Hpy8I GTNNAC 5 cut(s) 205, 295, 592, 757, 792
Hpy99I CGWCG 7 cut(s) 137, 146, 191, 206, 209, 314, 671
HpyAV CCTTC 6 cut(s) 70, 102, 254, 271, 850, 936
HpyCH4III ACNGT 3 cut(s) 310, 761, 896
HpyCH4IV ACGT 6 cut(s) 141, 226, 390, 486, 588, 969
HpyCH4V TGCA 5 cut(s) 327, 382, 607, 932, 973
HpyF10VI GCNNNNNNNGC 5 cut(s) 39, 144, 376, 827, 934
HpyF3I CTNAG 6 cut(s) 426, 597, 640, 814, 842, 1001
HpySE526I ACGT 6 cut(s) 141, 226, 390, 486, 588, 969
Hsp92I GRCGYC 2 cut(s) 132, 207
Hsp92II CATG 4 cut(s) 151, 574, 632, 801
HspAI GCGC 4 cut(s) 81, 106, 344, 346
Kpn2I TCCGGA 2 cut(s) 436, 503
KspAI GTTAAC 1 cut(s) 592
Kzo9I GATC 2 cut(s) 314, 577
LmnI GCTCC 1 cut(s) 834
Lsp1109I GCAGC 5 cut(s) 95, 244, 266, 366, 715
LweI GCATC 2 cut(s) 225, 336
MaeI CTAG 4 cut(s) 422, 555, 695, 827
MaeII ACGT 6 cut(s) 141, 226, 390, 486, 588, 969
MaeIII GTNAC 3 cut(s) 331, 809, 961
MalI GATC 2 cut(s) 316, 579
MboI GATC 2 cut(s) 314, 577
MboII GAAGA 2 cut(s) 55, 748
MfeI CAATTG 1 cut(s) 804
MflI RGATCY 1 cut(s) 314
MhlI GDGCHC 1 cut(s) 35
MlsI TGGCCA 1 cut(s) 152
MluCI AATT 1 cut(s) 804
MluNI TGGCCA 1 cut(s) 152
MlyI GAGTC 3 cut(s) 371, 542, 1001
MmeI TCCRAC 3 cut(s) 292, 342, 886
Mox20I TGGCCA 1 cut(s) 152
MroI TCCGGA 2 cut(s) 436, 503
MscI TGGCCA 1 cut(s) 152
MseI TTAA 3 cut(s) 170, 591, 1043
Msp20I TGGCCA 1 cut(s) 152
MspA1I CMGCKG 1 cut(s) 257
MspI CCGG 5 cut(s) 339, 437, 504, 680, 832
MspR9I CCNGG 6 cut(s) 36, 56, 357, 367, 584, 832
MunI CAATTG 1 cut(s) 804
MvaI CCWGG 5 cut(s) 36, 56, 357, 367, 584
MvnI CGCG 2 cut(s) 81, 346
MwoI GCNNNNNNNGC 5 cut(s) 39, 144, 376, 827, 934
NciI CCSGG 1 cut(s) 832
NdeII GATC 2 cut(s) 314, 577
NlaIII CATG 4 cut(s) 151, 574, 632, 801
NlaIV GGNNCC 3 cut(s) 33, 316, 566
NmeAIII GCCGAG 1 cut(s) 989
NmuCI GTSAC 2 cut(s) 331, 961
PauI GCGCGC 1 cut(s) 344
PceI AGGCCT 2 cut(s) 42, 59
PcsI WCGNNNNNNNCGW 1 cut(s) 306
PfeI GAWTC 2 cut(s) 541, 739
PkrI GCNGC 6 cut(s) 80, 85, 256, 259, 381, 705
PleI GAGTC 3 cut(s) 370, 542, 1001
PpsI GAGTC 3 cut(s) 370, 542, 1001
Ppu21I YACGTR 2 cut(s) 227, 487
Psp6I CCWGG 5 cut(s) 34, 54, 355, 365, 582
PspGI CCWGG 5 cut(s) 34, 54, 355, 365, 582
PspN4I GGNNCC 3 cut(s) 33, 316, 566
PspOMI GGGCCC 1 cut(s) 31
PspPI GGNCC 6 cut(s) 31, 32, 69, 241, 859, 1030
PstI CTGCAG 1 cut(s) 384
PsuI RGATCY 1 cut(s) 314
PteI GCGCGC 1 cut(s) 344
PvuII CAGCTG 1 cut(s) 257
RsaI GTAC 2 cut(s) 431, 538
RsaNI GTAC 2 cut(s) 430, 537
SalI GTCGAC 1 cut(s) 203
SaqAI TTAA 3 cut(s) 170, 591, 1043
SatI GCNGC 6 cut(s) 79, 84, 255, 258, 380, 704
Sau3AI GATC 2 cut(s) 314, 577
Sau96I GGNCC 6 cut(s) 31, 32, 69, 241, 859, 1030
ScaI AGTACT 1 cut(s) 431
SchI GAGTC 3 cut(s) 371, 542, 1001
ScrFI CCNGG 6 cut(s) 36, 56, 357, 367, 584, 832
SduI GDGCHC 1 cut(s) 35
SfaNI GCATC 2 cut(s) 225, 336
SfcI CTRYAG 1 cut(s) 380
SfiI GGCCNNNNNGGCC 1 cut(s) 39
SgrDI CGTCGACG 1 cut(s) 203
SinI GGWCC 3 cut(s) 241, 859, 1030
SmlI CTYRAG 1 cut(s) 919
SmoI CTYRAG 1 cut(s) 919
Sse9I AATT 1 cut(s) 804
SseBI AGGCCT 2 cut(s) 42, 59
SsiI CCGC 2 cut(s) 79, 89
SspMI CTAG 4 cut(s) 422, 555, 695, 827
StuI AGGCCT 2 cut(s) 42, 59
StyD4I CCNGG 6 cut(s) 34, 54, 355, 365, 582, 830
TaaI ACNGT 3 cut(s) 310, 761, 896
TaiI ACGT 6 cut(s) 144, 229, 393, 489, 591, 972
TaqI TCGA 2 cut(s) 204, 546
TasI AATT 1 cut(s) 804
TatI WGTACW 1 cut(s) 429
TauI GCSGC 1 cut(s) 81
TfiI GAWTC 2 cut(s) 541, 739
Tru1I TTAA 3 cut(s) 170, 591, 1043
Tru9I TTAA 3 cut(s) 170, 591, 1043
TscAI CASTG 5 cut(s) 474, 648, 735, 822, 879
TseFI GTSAC 2 cut(s) 331, 961
TseI GCWGC 5 cut(s) 83, 254, 257, 379, 703
Tsp45I GTSAC 2 cut(s) 331, 961
TspDTI ATGAA 2 cut(s) 17, 786
TspGWI ACGGA 2 cut(s) 112, 280
TspRI CASTG 5 cut(s) 474, 648, 735, 822, 879
VpaK11BI GGWCC 3 cut(s) 241, 859, 1030
XagI CCTNNNNNAGG 2 cut(s) 53, 64
XmiI GTMKAC 2 cut(s) 204, 791
XspI CTAG 4 cut(s) 422, 555, 695, 827
ZrmI AGTACT 1 cut(s) 431
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.