Prupe.1G339800_v2.0.a1
ERF Family

Belongs to the LDH MDH superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0005141
Physical Location & Seq
Forward (+)
31981979 .. 31983130
1152 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G339800.1

Sequence Viewer

Length: 1059 bp
ATGCATAAGAGTAGTTCAGGGTCATCACTAGGACCAGGAGGGCTGGACTTAACCCAGTCCTTCTTCAAACCCATATCCAGTGCAGCCCCTCCTTCCCCCACCAAGCGCCACACCAAGATCTCCGTCGTCGGCGCTGGTAACGTCGGCATGGCCATTGCCCAAACCATCTTAACCCAAGACCTCGCCGACGAGCTCGTCCTCATCGACGTCAATCCCGACAAGCTACGCGGGGAGATGCTCGACCTCCAGCACGCCGCCGCCTTCCTCCCCCGCACCAAAATCCTGGCCTCCGTCGACTACGCCGTCACCGTTGGCTCCGACCTCTGCATAGTCACCGCCGGTGCCCGCCAGAATCTGGGAGAGTCCAGGCTCAACCTGCTCCAGAGGAACGTCGCTCTCTTCCGGAAAATCATCCCCCCACTCGTCAAGTTCTCGCCGGATACCATCTTACTCATCGTATCTAACCCGGTGGACGTCTTGACTCACGTGGCGTGGAAGCTATCCGGGTTTCCCAGCAACCGGGTCCTGGGGTCGGGGACCAATCTGGACTCTTCCAGGTTCCGCTTCCTCATCGCTGATCATCTTGACGTGAACGCCCAAGATGTGCAGGCTTACATCGTTGGGGAGCATGGAGACAGCTCAGTGGCTCTGTGGTCAAGCATCAGCGTAGGGGGAGTGCCGGTGCTGAGCTTCCTAGAGAAGCAGGAAATAGCGTACGAGAAAGAGACACTGGAGGAGATCCACAAGGCAGTGGTGGACAGCGCGTATGAAGTGATAAGCCTGAAAGGGTATACCTCTTGGGCAATAGGGTACTCGGTGGCTAACCTAGCTCGGAGCATACTGAGGGACCAGAGGAAGATCCACCCAGTTTCGGTTCTGGCCAAGGGCTTCTATGGAGTTGAAGGCGGGGAAGTGTTCTTGAGCTTGCCTGCGCAGCTGGGGAGAGGTGGGGTGTTGGGGGTCACCAATATACATCTCACTGATGAGGAGGAACAGAGGCTCAGGGACTCTGCTCGCACCATTTTGGATGTCCAATCTCAGTTGAACTTGGGGATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko00270 ko00620 ko00640 ko01100 ko01110 ko01120 ko01130 ko04922 map00010 map00270 map00620 map00640 map01100 map01110 map01120 map01130 map04922
Pfam Domains
Protein Families
Ldh_1_C Ldh_1_N

Protein Analysis

353

Amino Acids

37.9

Weight (kDa)

6.15

Isoelectric Point (pI)

40.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 194, 302
AatII GACGTC 2 cut(s) 210, 477
Acc16I TGCGCA 1 cut(s) 933
Acc36I ACCTGC 1 cut(s) 384
AccB1I GGYRCC 1 cut(s) 341
AccB7I CCANNNNNTGG 1 cut(s) 355
AccI GTMKAC 2 cut(s) 294, 791
AccII CGCG 2 cut(s) 228, 764
AccIII TCCGGA 1 cut(s) 402
AciI CCGC 8 cut(s) 228, 255, 258, 271, 336, 346, 562, 906
AclWI GGATC 2 cut(s) 733, 853
AcoI YGGCCR 2 cut(s) 150, 879
AcvI CACGTG 1 cut(s) 487
AcyI GRCGYC 2 cut(s) 207, 474
AfaI GTAC 2 cut(s) 716, 812
AfiI CCNNNNNNNGG 5 cut(s) 355, 519, 526, 532, 871
AgsI TTSAA 3 cut(s) 67, 902, 1045
AjiI CACGTC 1 cut(s) 589
AjnI CCWGG 5 cut(s) 34, 282, 365, 525, 554
AluBI AGCT 8 cut(s) 193, 223, 499, 639, 690, 830, 924, 937
AluI AGCT 8 cut(s) 193, 223, 499, 639, 690, 830, 924, 937
Alw21I GWGCWC 1 cut(s) 195
Alw26I GTCTC 2 cut(s) 627, 719
AlwI GGATC 2 cut(s) 733, 853
AlwNI CAGNNNCTG 1 cut(s) 355
Aor13HI TCCGGA 1 cut(s) 402
AoxI GGCC 3 cut(s) 150, 285, 879
ApeKI GCWGC 2 cut(s) 83, 934
AspLEI GCGC 4 cut(s) 108, 134, 764, 934
AspS9I GGNCC 4 cut(s) 32, 523, 537, 847
AsuC2I CCSGG 3 cut(s) 467, 505, 521
AsuHPI GGTGA 3 cut(s) 298, 325, 955
AvaII GGWCC 4 cut(s) 32, 523, 537, 847
BaeGI GKGCMC 1 cut(s) 346
BalI TGGCCA 2 cut(s) 152, 881
BanI GGYRCC 1 cut(s) 341
BanII GRGCYC 1 cut(s) 195
BbrPI CACGTG 1 cut(s) 487
Bbv12I GWGCWC 1 cut(s) 195
BbvI GCAGC 2 cut(s) 95, 946
BccI CCATC 2 cut(s) 173, 452
BceAI ACGGC 1 cut(s) 287
BciT130I CCWGG 5 cut(s) 36, 284, 367, 527, 556
BciVI GTATCC 1 cut(s) 433
BclI TGATCA 1 cut(s) 577
BcnI CCSGG 3 cut(s) 467, 505, 521
BcoDI GTCTC 2 cut(s) 627, 719
BfaI CTAG 3 cut(s) 29, 695, 827
BfoI RGCGCY 2 cut(s) 109, 135
BfuAI ACCTGC 1 cut(s) 384
BfuI GTATCC 1 cut(s) 433
BglII AGATCT 1 cut(s) 117
BisI GCNGC 4 cut(s) 84, 255, 258, 935
BlpI GCTNAGC 1 cut(s) 686
BlsI GCNGC 4 cut(s) 85, 256, 259, 936
Bme1390I CCNGG 8 cut(s) 36, 284, 367, 467, 505, 521, 527, 556
Bme18I GGWCC 4 cut(s) 32, 523, 537, 847
BmgBI CACGTC 1 cut(s) 589
BmgT120I GGNCC 4 cut(s) 32, 523, 537, 847
BmiI GGNNCC 6 cut(s) 316, 343, 524, 538, 560, 848
BmrFI CCNGG 8 cut(s) 36, 284, 367, 467, 505, 521, 527, 556
BmrI ACTGGG 2 cut(s) 49, 860
BmsI GCATC 2 cut(s) 225, 669
BmuI ACTGGG 2 cut(s) 49, 860
BpmI CTGGAG 3 cut(s) 230, 365, 752
Bpu10I CCTNAGC 1 cut(s) 1001
Bpu1102I GCTNAGC 1 cut(s) 686
BpuEI CTTGAG 1 cut(s) 940
BpuMI CCSGG 3 cut(s) 467, 505, 521
BsaAI YACGTR 1 cut(s) 487
BsaHI GRCGYC 2 cut(s) 207, 474
BsaJI CCNNGG 2 cut(s) 526, 882
BsaWI WCCGGW 1 cut(s) 402
BsaXI ACNNNNNCTCC 2 cut(s) 666, 696
Bsc4I CCNNNNNNNGG 5 cut(s) 355, 519, 526, 532, 871
Bse118I RCCGGY 2 cut(s) 338, 679
Bse1I ACTGG 4 cut(s) 55, 78, 735, 866
Bse3DI GCAATG 1 cut(s) 153
BseAI TCCGGA 1 cut(s) 402
BseBI CCWGG 5 cut(s) 36, 284, 367, 527, 556
BseDI CCNNGG 2 cut(s) 526, 882
BseGI GGATG 2 cut(s) 411, 1033
BseLI CCNNNNNNNGG 5 cut(s) 355, 519, 526, 532, 871
BseMI GCAATG 1 cut(s) 153
BseMII CTCAG 5 cut(s) 654, 677, 833, 1015, 1052
BseNI ACTGG 4 cut(s) 55, 78, 735, 866
BseRI GAGGAG 2 cut(s) 749, 1001
BseSI GKGCMC 1 cut(s) 346
BseXI GCAGC 2 cut(s) 95, 946
BseYI CCCAGC 2 cut(s) 512, 937
BsgI GTGCAG 2 cut(s) 102, 626
Bsh1236I CGCG 2 cut(s) 228, 764
BshFI GGCC 3 cut(s) 152, 287, 881
BshNI GGYRCC 1 cut(s) 341
BsiHKAI GWGCWC 1 cut(s) 195
BsiSI CCGG 7 cut(s) 339, 403, 437, 467, 504, 520, 680
BsiWI CGTACG 1 cut(s) 714
BslFI GGGAC 3 cut(s) 550, 860, 1019
BslI CCNNNNNNNGG 5 cut(s) 355, 519, 526, 532, 871
BsmAI GTCTC 2 cut(s) 627, 719
BsmFI GGGAC 3 cut(s) 550, 860, 1019
BsnI GGCC 3 cut(s) 152, 287, 881
Bsp1286I GDGCHC 2 cut(s) 195, 346
Bsp13I TCCGGA 1 cut(s) 402
Bsp143I GATC 4 cut(s) 117, 577, 738, 858
Bsp1720I GCTNAGC 1 cut(s) 686
BspACI CCGC 8 cut(s) 228, 255, 258, 271, 336, 346, 562, 906
BspANI GGCC 3 cut(s) 152, 287, 881
BspCNI CTCAG 5 cut(s) 653, 678, 834, 1014, 1051
BspEI TCCGGA 1 cut(s) 402
BspFNI CGCG 2 cut(s) 228, 764
BspLI GGNNCC 6 cut(s) 316, 343, 524, 538, 560, 848
BspMI ACCTGC 1 cut(s) 384
BspPI GGATC 2 cut(s) 733, 853
BspT107I GGYRCC 1 cut(s) 341
BsrDI GCAATG 1 cut(s) 153
BsrFI RCCGGY 2 cut(s) 338, 679
BsrI ACTGG 4 cut(s) 55, 78, 735, 866
BssAI RCCGGY 2 cut(s) 338, 679
BssECI CCNNGG 2 cut(s) 526, 882
BssMI GATC 4 cut(s) 117, 577, 738, 858
BssNAI GTATAC 1 cut(s) 792
BssNI GRCGYC 2 cut(s) 207, 474
BssT1I CCWWGG 1 cut(s) 882
Bst1107I GTATAC 1 cut(s) 792
Bst2UI CCWGG 5 cut(s) 36, 284, 367, 527, 556
Bst4CI ACNGT 1 cut(s) 310
Bst6I CTCTTC 2 cut(s) 404, 556
BstACI GRCGYC 2 cut(s) 207, 474
BstBAI YACGTR 1 cut(s) 487
BstC8I GCNNGC 6 cut(s) 252, 346, 609, 926, 930, 1015
BstDEI CTNAG 5 cut(s) 640, 686, 842, 1001, 1038
BstEII GGTNACC 1 cut(s) 961
BstF5I GGATG 2 cut(s) 411, 1033
BstFNI CGCG 2 cut(s) 228, 764
BstH2I RGCGCY 2 cut(s) 109, 135
BstHHI GCGC 4 cut(s) 108, 134, 764, 934
BstKTI GATC 4 cut(s) 120, 580, 741, 861
BstMAI GTCTC 2 cut(s) 627, 719
BstMBI GATC 4 cut(s) 117, 577, 738, 858
BstMWI GCNNNNNNNGC 3 cut(s) 376, 827, 934
BstNI CCWGG 5 cut(s) 36, 284, 367, 527, 556
BstPI GGTNACC 1 cut(s) 961
BstSCI CCNGG 8 cut(s) 34, 282, 365, 465, 503, 519, 525, 554
BstSLI GKGCMC 1 cut(s) 346
BstUI CGCG 2 cut(s) 228, 764
BstV1I GCAGC 2 cut(s) 95, 946
BstX2I RGATCY 3 cut(s) 117, 738, 858
BstXI CCANNNNNNTGG 1 cut(s) 283
BstYI RGATCY 3 cut(s) 117, 738, 858
BstZ17I GTATAC 1 cut(s) 792
BsuI GTATCC 1 cut(s) 433
BsuRI GGCC 3 cut(s) 152, 287, 881
BtgZI GCGATG 1 cut(s) 556
BtrI CACGTC 1 cut(s) 589
BtsCI GGATG 2 cut(s) 411, 1033
BtsI GCAGTG 1 cut(s) 756
BtsIMutI CAGTG 5 cut(s) 85, 648, 728, 756, 978
BveI ACCTGC 1 cut(s) 384
Cac8I GCNNGC 6 cut(s) 252, 346, 609, 926, 930, 1015
CaiI CAGNNNCTG 1 cut(s) 355
CfoI GCGC 4 cut(s) 108, 134, 764, 934
Cfr10I RCCGGY 2 cut(s) 338, 679
Cfr13I GGNCC 4 cut(s) 32, 523, 537, 847
Csp6I GTAC 2 cut(s) 715, 811
CviAII CATG 2 cut(s) 148, 629
CviQI GTAC 2 cut(s) 715, 811
DdeI CTNAG 5 cut(s) 640, 686, 842, 1001, 1038
DpnI GATC 4 cut(s) 119, 579, 740, 860
DpnII GATC 4 cut(s) 117, 577, 738, 858
DrdI GACNNNNNNGTC 2 cut(s) 194, 302
DseDI GACNNNNNNGTC 2 cut(s) 194, 302
EaeI YGGCCR 2 cut(s) 150, 879
Eam1104I CTCTTC 2 cut(s) 404, 556
EarI CTCTTC 2 cut(s) 404, 556
Ecl136II GAGCTC 1 cut(s) 193
Eco130I CCWWGG 1 cut(s) 882
Eco24I GRGCYC 1 cut(s) 195
Eco47I GGWCC 4 cut(s) 32, 523, 537, 847
Eco53kI GAGCTC 1 cut(s) 193
Eco72I CACGTG 1 cut(s) 487
Eco91I GGTNACC 1 cut(s) 961
EcoICRI GAGCTC 1 cut(s) 193
EcoO109I RGGNCCY 1 cut(s) 523
EcoO65I GGTNACC 1 cut(s) 961
EcoRII CCWGG 5 cut(s) 34, 282, 365, 525, 554
EcoT14I CCWWGG 1 cut(s) 882
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 195
ErhI CCWWGG 1 cut(s) 882
FaeI CATG 2 cut(s) 151, 632
FaqI GGGAC 3 cut(s) 550, 860, 1019
FatI CATG 2 cut(s) 147, 628
FauI CCCGC 4 cut(s) 221, 278, 353, 899
FbaI TGATCA 1 cut(s) 577
FblI GTMKAC 2 cut(s) 294, 791
Fnu4HI GCNGC 4 cut(s) 84, 255, 258, 935
FokI GGATG 2 cut(s) 398, 1040
FriOI GRGCYC 1 cut(s) 195
Fsp4HI GCNGC 4 cut(s) 84, 255, 258, 935
FspBI CTAG 3 cut(s) 29, 695, 827
FspI TGCGCA 1 cut(s) 933
GlaI GCGC 4 cut(s) 107, 133, 763, 933
GluI GCNGC 4 cut(s) 84, 255, 258, 935
GsaI CCCAGC 2 cut(s) 516, 941
GsuI CTGGAG 3 cut(s) 230, 365, 752
HaeII RGCGCY 2 cut(s) 109, 135
HaeIII GGCC 3 cut(s) 152, 287, 881
HapII CCGG 7 cut(s) 339, 403, 437, 467, 504, 520, 680
HhaI GCGC 4 cut(s) 108, 134, 764, 934
Hin1I GRCGYC 2 cut(s) 207, 474
Hin1II CATG 2 cut(s) 151, 632
Hin6I GCGC 4 cut(s) 106, 132, 762, 932
HinP1I GCGC 4 cut(s) 106, 132, 762, 932
HincII GTYRAC 1 cut(s) 295
HindII GTYRAC 1 cut(s) 295
HinfI GANTC 5 cut(s) 352, 362, 481, 548, 1007
HpaII CCGG 7 cut(s) 339, 403, 437, 467, 504, 520, 680
HphI GGTGA 3 cut(s) 298, 325, 955
Hpy166II GTNNAC 5 cut(s) 295, 472, 592, 757, 792
Hpy188I TCNGA 2 cut(s) 319, 834
Hpy188III TCNNGA 7 cut(s) 215, 382, 403, 478, 545, 584, 919
Hpy8I GTNNAC 5 cut(s) 295, 472, 592, 757, 792
Hpy99I CGWCG 7 cut(s) 128, 131, 146, 191, 209, 296, 395
HpyAV CCTTC 4 cut(s) 70, 102, 271, 896
HpyCH4III ACNGT 1 cut(s) 310
HpyCH4IV ACGT 6 cut(s) 141, 207, 390, 474, 486, 588
HpyCH4V TGCA 4 cut(s) 4, 83, 327, 607
HpyF10VI GCNNNNNNNGC 3 cut(s) 376, 827, 934
HpyF3I CTNAG 5 cut(s) 640, 686, 842, 1001, 1038
HpySE526I ACGT 6 cut(s) 141, 207, 390, 474, 486, 588
Hsp92I GRCGYC 2 cut(s) 207, 474
Hsp92II CATG 2 cut(s) 151, 632
HspAI GCGC 4 cut(s) 106, 132, 762, 932
Kpn2I TCCGGA 1 cut(s) 402
Ksp22I TGATCA 1 cut(s) 577
Kzo9I GATC 4 cut(s) 117, 577, 738, 858
LmnI GCTCC 4 cut(s) 320, 384, 625, 834
Lsp1109I GCAGC 2 cut(s) 95, 946
LweI GCATC 2 cut(s) 225, 669
MaeI CTAG 3 cut(s) 29, 695, 827
MaeII ACGT 6 cut(s) 141, 207, 390, 474, 486, 588
MaeIII GTNAC 4 cut(s) 137, 304, 331, 961
MalI GATC 4 cut(s) 119, 579, 740, 860
MboI GATC 4 cut(s) 117, 577, 738, 858
MboII GAAGA 4 cut(s) 55, 391, 543, 868
MflI RGATCY 3 cut(s) 117, 738, 858
MhlI GDGCHC 2 cut(s) 195, 346
MlsI TGGCCA 2 cut(s) 152, 881
MluNI TGGCCA 2 cut(s) 152, 881
MlyI GAGTC 4 cut(s) 371, 475, 542, 1001
MmeI TCCRAC 1 cut(s) 342
Mox20I TGGCCA 2 cut(s) 152, 881
Mph1103I ATGCAT 1 cut(s) 6
MroI TCCGGA 1 cut(s) 402
MscI TGGCCA 2 cut(s) 152, 881
MseI TTAA 2 cut(s) 50, 170
Msp20I TGGCCA 2 cut(s) 152, 881
MspA1I CMGCKG 1 cut(s) 937
MspI CCGG 7 cut(s) 339, 403, 437, 467, 504, 520, 680
MspR9I CCNGG 8 cut(s) 36, 284, 367, 467, 505, 521, 527, 556
MvaI CCWGG 5 cut(s) 36, 284, 367, 527, 556
MvnI CGCG 2 cut(s) 228, 764
MwoI GCNNNNNNNGC 3 cut(s) 376, 827, 934
NciI CCSGG 3 cut(s) 467, 505, 521
NdeII GATC 4 cut(s) 117, 577, 738, 858
NlaIII CATG 2 cut(s) 151, 632
NlaIV GGNNCC 6 cut(s) 316, 343, 524, 538, 560, 848
NmuCI GTSAC 3 cut(s) 304, 331, 961
NsbI TGCGCA 1 cut(s) 933
NsiI ATGCAT 1 cut(s) 6
PcsI WCGNNNNNNNCGW 4 cut(s) 201, 213, 300, 306
PfeI GAWTC 1 cut(s) 352
Pfl23II CGTACG 1 cut(s) 714
PflMI CCANNNNNTGG 1 cut(s) 355
PkrI GCNGC 4 cut(s) 85, 256, 259, 936
PleI GAGTC 4 cut(s) 370, 475, 542, 1001
PmaCI CACGTG 1 cut(s) 487
PmlI CACGTG 1 cut(s) 487
PpsI GAGTC 4 cut(s) 370, 475, 542, 1001
Ppu21I YACGTR 1 cut(s) 487
PpuMI RGGWCCY 1 cut(s) 523
Psp124BI GAGCTC 1 cut(s) 195
Psp5II RGGWCCY 1 cut(s) 523
Psp6I CCWGG 5 cut(s) 34, 282, 365, 525, 554
PspCI CACGTG 1 cut(s) 487
PspEI GGTNACC 1 cut(s) 961
PspFI CCCAGC 2 cut(s) 512, 937
PspGI CCWGG 5 cut(s) 34, 282, 365, 525, 554
PspLI CGTACG 1 cut(s) 714
PspN4I GGNNCC 6 cut(s) 316, 343, 524, 538, 560, 848
PspPI GGNCC 4 cut(s) 32, 523, 537, 847
PspPPI RGGWCCY 1 cut(s) 523
PstNI CAGNNNCTG 1 cut(s) 355
PsuI RGATCY 3 cut(s) 117, 738, 858
PvuII CAGCTG 1 cut(s) 937
RsaI GTAC 2 cut(s) 716, 812
RsaNI GTAC 2 cut(s) 715, 811
SacI GAGCTC 1 cut(s) 195
SalI GTCGAC 1 cut(s) 293
SaqAI TTAA 2 cut(s) 50, 170
SatI GCNGC 4 cut(s) 84, 255, 258, 935
Sau3AI GATC 4 cut(s) 117, 577, 738, 858
Sau96I GGNCC 4 cut(s) 32, 523, 537, 847
SchI GAGTC 4 cut(s) 371, 475, 542, 1001
ScrFI CCNGG 8 cut(s) 36, 284, 367, 467, 505, 521, 527, 556
SduI GDGCHC 2 cut(s) 195, 346
SfaNI GCATC 2 cut(s) 225, 669
SgrAI CRCCGGYG 1 cut(s) 338
SinI GGWCC 4 cut(s) 32, 523, 537, 847
SmlI CTYRAG 1 cut(s) 919
SmoI CTYRAG 1 cut(s) 919
SsiI CCGC 8 cut(s) 228, 255, 258, 271, 336, 346, 562, 906
SspMI CTAG 3 cut(s) 29, 695, 827
SstI GAGCTC 1 cut(s) 195
StyD4I CCNGG 8 cut(s) 34, 282, 365, 465, 503, 519, 525, 554
StyI CCWWGG 1 cut(s) 882
TaaI ACNGT 1 cut(s) 310
TaiI ACGT 6 cut(s) 144, 210, 393, 477, 489, 591
TaqI TCGA 3 cut(s) 204, 240, 294
TauI GCSGC 2 cut(s) 257, 260
TfiI GAWTC 1 cut(s) 352
Tru1I TTAA 2 cut(s) 50, 170
Tru9I TTAA 2 cut(s) 50, 170
TscAI CASTG 5 cut(s) 85, 648, 735, 756, 985
TseFI GTSAC 3 cut(s) 304, 331, 961
TseI GCWGC 2 cut(s) 83, 934
Tsp45I GTSAC 3 cut(s) 304, 331, 961
TspDTI ATGAA 1 cut(s) 783
TspGWI ACGGA 2 cut(s) 112, 280
TspRI CASTG 5 cut(s) 85, 648, 735, 756, 985
Van91I CCANNNNNTGG 1 cut(s) 355
VpaK11BI GGWCC 4 cut(s) 32, 523, 537, 847
XmiI GTMKAC 2 cut(s) 294, 791
XspI CTAG 3 cut(s) 29, 695, 827
ZraI GACGTC 2 cut(s) 208, 475
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.