Rh4BG435800
ERF Family

Belongs to the LDH MDH superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0005141
Physical Location & Seq
Forward (+)
57750403 .. 57751998
1596 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG435800.1

Sequence Viewer

Length: 1059 bp
ATGAACAAAAGCAGTTCGGCTTCGTCATTGGGCCCTGGAGGCCTTGACCTAACCCAGGCCTTCTTCAGGCCCATCAGCCGCGCAGCCCCGCCTTCCCCGACCAAGCGCCACAACAAAATCTCTGTCATTGGCGTCGGCAACGTCGGCATGGCCATAGCACAAACCATCTTAACCCAAGACCTCGCCGACGAGCTTGTCCTCGTCGACGCCAAACCTGAAAAGCTACGTGGAGAGATGCTGGACCTTCAACACGCAGCTGCCTTCTTACCCCGCACCAAAATCCACGCCTCCGTGGACTACGCCATCACCGTCGGATCCGACCTCTGCATCGTGACGGCCGGTGCGCGCCAGAACCCTGGCGAGTCCAGGCTGAACCTGCTGCAGAGGAACGTGGCTCTCTTTAGAAATATCATACCTCCACTAGCTAAGTACTCTCCGGACACCATATTACTCATCGTGTCCAATCCAGTGGATGTCCTGACTTACGTGGCGTGGAAGCTATCCGGATTTCCGAGCAACAGGGTTTTCGGGTCGGGTACGAATCTCGACTCATCTAGGTTTCGGTTCCTCATGGCCGATCACCTGGACGTTAACGCTCAGGATGTGCAGGCTTACATCATTGGCGAGCATGGGGACAGCTCAGTGGCTTTGTGGTCAAGCATTAGCGTCGGGGGAGTGCCGGTGTTGAGCTTCCTAGAGAAGCAGCAAATAGCATACGAGAAAGAGACACTGGAGAAGATTCACAAAGCAGTTGTGGACTGTGCGTATGAGGTGATAAGTCTGAAAGGGTATACTTCATGGGCAATTGGTTACTCAGTGGCAAACCTAGCCCGGAGCATACTCAGAGACCAGAGGAAGGTCCAACCTGTTTCAGTGCTTGCCAATGGGTTCTACGGTGTTGGTGGAGGTGATGTGTTCTTGAGCTTGCCTGCACAGCTCGGAAGGAGTGGAGTTCTGGGAGTGACCAATGTGCATTTGACTGATGAGGAGGCACAGAAGCTAAGGGACTCGGCTCACACCATTTTGGAGGTCCAAAGTCAGTTAAACTTGGGAATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko00270 ko00620 ko00640 ko01100 ko01110 ko01120 ko01130 ko04922 map00010 map00270 map00620 map00640 map01100 map01110 map01120 map01130 map04922
Pfam Domains
Protein Families
Ldh_1_C Ldh_1_N

Protein Analysis

352

Amino Acids

37.91

Weight (kDa)

7.1

Isoelectric Point (pI)

39.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ldh_1_N PF00056 39 - 177 2.1e-44 lactate/malate dehydrogenase, NAD binding domain
Ldh_1_C PF02866 180 - 343 1.8e-32 lactate/malate dehydrogenase, alpha/beta C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 194
Acc36I ACCTGC 1 cut(s) 384
AccI GTMKAC 2 cut(s) 204, 791
AccII CGCG 2 cut(s) 81, 346
AccIII TCCGGA 2 cut(s) 436, 503
AciI CCGC 3 cut(s) 79, 89, 271
AclWI GGATC 2 cut(s) 309, 322
AcoI YGGCCR 3 cut(s) 150, 336, 573
AcuI CTGAAG 1 cut(s) 49
AcyI GRCGYC 2 cut(s) 132, 207
AfaI GTAC 2 cut(s) 431, 538
AfiI CCNNNNNNNGG 3 cut(s) 55, 66, 856
AgsI TTSAA 1 cut(s) 248
AjnI CCWGG 5 cut(s) 34, 54, 355, 365, 582
Alw26I GTCTC 2 cut(s) 719, 840
AlwI GGATC 2 cut(s) 309, 322
Aor13HI TCCGGA 2 cut(s) 436, 503
AoxI GGCC 7 cut(s) 31, 40, 57, 68, 150, 336, 573
ApaI GGGCCC 1 cut(s) 35
ApeKI GCWGC 5 cut(s) 83, 254, 257, 379, 703
ArsI GACNNNNNNTTYG 2 cut(s) 108, 140
AspLEI GCGC 4 cut(s) 83, 108, 346, 348
AspS9I GGNCC 6 cut(s) 31, 32, 69, 241, 859, 1030
AsuC2I CCSGG 1 cut(s) 832
AsuHPI GGTGA 4 cut(s) 298, 572, 784, 920
AvaII GGWCC 3 cut(s) 241, 859, 1030
BaeGI GKGCMC 1 cut(s) 35
BalI TGGCCA 1 cut(s) 152
BamHI GGATCC 1 cut(s) 314
BanII GRGCYC 1 cut(s) 35
BbvI GCAGC 5 cut(s) 95, 244, 266, 366, 715
BccI CCATC 3 cut(s) 80, 173, 311
BceAI ACGGC 1 cut(s) 351
BcgI CGANNNNNNTGC 2 cut(s) 649, 683
BciT130I CCWGG 5 cut(s) 36, 56, 357, 367, 584
BcnI CCSGG 1 cut(s) 832
BcoDI GTCTC 2 cut(s) 719, 840
BfaI CTAG 4 cut(s) 422, 555, 695, 827
BfmI CTRYAG 1 cut(s) 380
BfoI RGCGCY 1 cut(s) 109
BfuAI ACCTGC 1 cut(s) 384
BglI GCCNNNNNGGC 1 cut(s) 39
BisI GCNGC 6 cut(s) 79, 84, 255, 258, 380, 704
BlsI GCNGC 6 cut(s) 80, 85, 256, 259, 381, 705
BmcAI AGTACT 1 cut(s) 431
Bme1390I CCNGG 6 cut(s) 36, 56, 357, 367, 584, 832
Bme18I GGWCC 3 cut(s) 241, 859, 1030
BmgT120I GGNCC 6 cut(s) 31, 32, 69, 241, 859, 1030
BmiI GGNNCC 3 cut(s) 33, 316, 566
BmrFI CCNGG 6 cut(s) 36, 56, 357, 367, 584, 832
BmsI GCATC 2 cut(s) 225, 336
BpmI CTGGAG 2 cut(s) 57, 752
Bpu10I CCTNAGC 2 cut(s) 597, 1001
BpuEI CTTGAG 1 cut(s) 940
BpuMI CCSGG 1 cut(s) 832
BsaAI YACGTR 2 cut(s) 227, 487
BsaHI GRCGYC 2 cut(s) 132, 207
BsaI GGTCTC 1 cut(s) 840
BsaJI CCNNGG 4 cut(s) 34, 54, 291, 355
BsaWI WCCGGW 2 cut(s) 436, 503
BsaXI ACNNNNNCTCC 4 cut(s) 666, 696, 897, 927
Bsc4I CCNNNNNNNGG 3 cut(s) 55, 66, 856
Bse118I RCCGGY 2 cut(s) 338, 679
Bse1I ACTGG 2 cut(s) 467, 735
BseAI TCCGGA 2 cut(s) 436, 503
BseBI CCWGG 5 cut(s) 36, 56, 357, 367, 584
BseDI CCNNGG 4 cut(s) 34, 54, 291, 355
BseGI GGATG 2 cut(s) 478, 607
BseLI CCNNNNNNNGG 3 cut(s) 55, 66, 856
BseMII CTCAG 4 cut(s) 611, 654, 828, 856
BseNI ACTGG 2 cut(s) 467, 735
BsePI GCGCGC 1 cut(s) 344
BseRI GAGGAG 1 cut(s) 1001
BseSI GKGCMC 1 cut(s) 35
BseX3I CGGCCG 1 cut(s) 336
BseXI GCAGC 5 cut(s) 95, 244, 266, 366, 715
BsgI GTGCAG 2 cut(s) 626, 915
Bsh1236I CGCG 2 cut(s) 81, 346
Bsh1285I CGRYCG 1 cut(s) 339
BshFI GGCC 7 cut(s) 33, 42, 59, 70, 152, 338, 575
BsiEI CGRYCG 1 cut(s) 339
BsiSI CCGG 5 cut(s) 339, 437, 504, 680, 832
BslFI GGGAC 2 cut(s) 647, 1019
BslI CCNNNNNNNGG 3 cut(s) 55, 66, 856
BsmAI GTCTC 2 cut(s) 719, 840
BsmFI GGGAC 2 cut(s) 647, 1019
BsnI GGCC 7 cut(s) 33, 42, 59, 70, 152, 338, 575
Bso31I GGTCTC 1 cut(s) 840
Bsp120I GGGCCC 1 cut(s) 31
Bsp1286I GDGCHC 1 cut(s) 35
Bsp13I TCCGGA 2 cut(s) 436, 503
Bsp143I GATC 2 cut(s) 314, 577
BspACI CCGC 3 cut(s) 79, 89, 271
BspANI GGCC 7 cut(s) 33, 42, 59, 70, 152, 338, 575
BspCNI CTCAG 4 cut(s) 610, 653, 827, 855
BspEI TCCGGA 2 cut(s) 436, 503
BspFNI CGCG 2 cut(s) 81, 346
BspLI GGNNCC 3 cut(s) 33, 316, 566
BspMAI CTGCAG 1 cut(s) 384
BspMI ACCTGC 1 cut(s) 384
BspPI GGATC 2 cut(s) 309, 322
BspTNI GGTCTC 1 cut(s) 840
BsrFI RCCGGY 2 cut(s) 338, 679
BsrI ACTGG 2 cut(s) 467, 735
BssAI RCCGGY 2 cut(s) 338, 679
BssECI CCNNGG 4 cut(s) 34, 54, 291, 355
BssHII GCGCGC 1 cut(s) 344
BssMI GATC 2 cut(s) 314, 577
BssNAI GTATAC 1 cut(s) 792
BssNI GRCGYC 2 cut(s) 132, 207
Bst1107I GTATAC 1 cut(s) 792
Bst2UI CCWGG 5 cut(s) 36, 56, 357, 367, 584
Bst4CI ACNGT 3 cut(s) 310, 761, 896
BstACI GRCGYC 2 cut(s) 132, 207
BstBAI YACGTR 2 cut(s) 227, 487
BstC8I GCNNGC 6 cut(s) 346, 609, 626, 879, 926, 930
BstDEI CTNAG 6 cut(s) 426, 597, 640, 814, 842, 1001
BstDSI CCRYGG 1 cut(s) 291
BstENI CCTNNNNNAGG 2 cut(s) 53, 64
BstF5I GGATG 2 cut(s) 478, 607
BstFNI CGCG 2 cut(s) 81, 346
BstH2I RGCGCY 1 cut(s) 109
BstHHI GCGC 4 cut(s) 83, 108, 346, 348
BstKTI GATC 2 cut(s) 317, 580
BstMAI GTCTC 2 cut(s) 719, 840
BstMBI GATC 2 cut(s) 314, 577
BstMCI CGRYCG 1 cut(s) 339
BstMWI GCNNNNNNNGC 5 cut(s) 39, 144, 376, 827, 934
BstNI CCWGG 5 cut(s) 36, 56, 357, 367, 584
BstSCI CCNGG 6 cut(s) 34, 54, 355, 365, 582, 830
BstSFI CTRYAG 1 cut(s) 380
BstSLI GKGCMC 1 cut(s) 35
BstUI CGCG 2 cut(s) 81, 346
BstV1I GCAGC 5 cut(s) 95, 244, 266, 366, 715
BstX2I RGATCY 1 cut(s) 314
BstXI CCANNNNNNTGG 2 cut(s) 356, 469
BstYI RGATCY 1 cut(s) 314
BstZ17I GTATAC 1 cut(s) 792
BstZI CGGCCG 1 cut(s) 336
BsuRI GGCC 7 cut(s) 33, 42, 59, 70, 152, 338, 575
BtgI CCRYGG 1 cut(s) 291
BtsCI GGATG 2 cut(s) 478, 607
BtsIMutI CAGTG 5 cut(s) 474, 648, 728, 822, 879
BveI ACCTGC 1 cut(s) 384
Cac8I GCNNGC 6 cut(s) 346, 609, 626, 879, 926, 930
CfoI GCGC 4 cut(s) 83, 108, 346, 348
Cfr10I RCCGGY 2 cut(s) 338, 679
Cfr13I GGNCC 6 cut(s) 31, 32, 69, 241, 859, 1030
CseI GACGC 3 cut(s) 121, 215, 655
Csp6I GTAC 2 cut(s) 430, 537
CviAII CATG 4 cut(s) 148, 571, 629, 798
CviQI GTAC 2 cut(s) 430, 537
DdeI CTNAG 6 cut(s) 426, 597, 640, 814, 842, 1001
DpnI GATC 2 cut(s) 316, 579
DpnII GATC 2 cut(s) 314, 577
DrdI GACNNNNNNGTC 1 cut(s) 194
DseDI GACNNNNNNGTC 1 cut(s) 194
EaeI YGGCCR 3 cut(s) 150, 336, 573
EagI CGGCCG 1 cut(s) 336
EclXI CGGCCG 1 cut(s) 336
Eco147I AGGCCT 2 cut(s) 42, 59
Eco24I GRGCYC 1 cut(s) 35
Eco31I GGTCTC 1 cut(s) 840
Eco47I GGWCC 3 cut(s) 241, 859, 1030
Eco52I CGGCCG 1 cut(s) 336
Eco57I CTGAAG 1 cut(s) 49
EcoNI CCTNNNNNAGG 2 cut(s) 53, 64
EcoO109I RGGNCCY 1 cut(s) 32
EcoRII CCWGG 5 cut(s) 34, 54, 355, 365, 582
EcoT38I GRGCYC 1 cut(s) 35
FaeI CATG 4 cut(s) 151, 574, 632, 801
FaqI GGGAC 2 cut(s) 647, 1019
FatI CATG 4 cut(s) 147, 570, 628, 797
FauI CCCGC 2 cut(s) 96, 278
FblI GTMKAC 2 cut(s) 204, 791
Fnu4HI GCNGC 6 cut(s) 79, 84, 255, 258, 380, 704
FokI GGATG 2 cut(s) 485, 614
FriOI GRGCYC 1 cut(s) 35
Fsp4HI GCNGC 6 cut(s) 79, 84, 255, 258, 380, 704
FspBI CTAG 4 cut(s) 422, 555, 695, 827
GlaI GCGC 4 cut(s) 82, 107, 345, 347
GluI GCNGC 6 cut(s) 79, 84, 255, 258, 380, 704
GsuI CTGGAG 2 cut(s) 57, 752
HaeII RGCGCY 1 cut(s) 109
HaeIII GGCC 7 cut(s) 33, 42, 59, 70, 152, 338, 575
HapII CCGG 5 cut(s) 339, 437, 504, 680, 832
HgaI GACGC 3 cut(s) 121, 215, 655
HhaI GCGC 4 cut(s) 83, 108, 346, 348
Hin1I GRCGYC 2 cut(s) 132, 207
Hin1II CATG 4 cut(s) 151, 574, 632, 801
Hin6I GCGC 4 cut(s) 81, 106, 344, 346
HinP1I GCGC 4 cut(s) 81, 106, 344, 346
HincII GTYRAC 2 cut(s) 205, 592
HindII GTYRAC 2 cut(s) 205, 592
HinfI GANTC 5 cut(s) 362, 541, 548, 739, 1007
HpaI GTTAAC 1 cut(s) 592
HpaII CCGG 5 cut(s) 339, 437, 504, 680, 832
HphI GGTGA 4 cut(s) 298, 572, 784, 920
Hpy166II GTNNAC 5 cut(s) 205, 295, 592, 757, 792
Hpy188I TCNGA 6 cut(s) 314, 319, 513, 783, 845, 941
Hpy188III TCNNGA 7 cut(s) 331, 437, 478, 504, 545, 599, 919
Hpy8I GTNNAC 5 cut(s) 205, 295, 592, 757, 792
Hpy99I CGWCG 7 cut(s) 137, 146, 191, 206, 209, 314, 671
HpyAV CCTTC 6 cut(s) 70, 102, 254, 271, 850, 936
HpyCH4III ACNGT 3 cut(s) 310, 761, 896
HpyCH4IV ACGT 5 cut(s) 141, 226, 390, 486, 588
HpyCH4V TGCA 5 cut(s) 327, 382, 607, 932, 973
HpyF10VI GCNNNNNNNGC 5 cut(s) 39, 144, 376, 827, 934
HpyF3I CTNAG 6 cut(s) 426, 597, 640, 814, 842, 1001
HpySE526I ACGT 5 cut(s) 141, 226, 390, 486, 588
Hsp92I GRCGYC 2 cut(s) 132, 207
Hsp92II CATG 4 cut(s) 151, 574, 632, 801
HspAI GCGC 4 cut(s) 81, 106, 344, 346
Kpn2I TCCGGA 2 cut(s) 436, 503
KspAI GTTAAC 1 cut(s) 592
Kzo9I GATC 2 cut(s) 314, 577
LmnI GCTCC 1 cut(s) 834
Lsp1109I GCAGC 5 cut(s) 95, 244, 266, 366, 715
LweI GCATC 2 cut(s) 225, 336
MaeI CTAG 4 cut(s) 422, 555, 695, 827
MaeII ACGT 5 cut(s) 141, 226, 390, 486, 588
MaeIII GTNAC 3 cut(s) 331, 809, 961
MalI GATC 2 cut(s) 316, 579
MboI GATC 2 cut(s) 314, 577
MboII GAAGA 2 cut(s) 55, 748
MfeI CAATTG 1 cut(s) 804
MflI RGATCY 1 cut(s) 314
MhlI GDGCHC 1 cut(s) 35
MlsI TGGCCA 1 cut(s) 152
MluCI AATT 1 cut(s) 804
MluNI TGGCCA 1 cut(s) 152
MlyI GAGTC 3 cut(s) 371, 542, 1001
MmeI TCCRAC 3 cut(s) 292, 342, 886
Mox20I TGGCCA 1 cut(s) 152
MroI TCCGGA 2 cut(s) 436, 503
MscI TGGCCA 1 cut(s) 152
MseI TTAA 3 cut(s) 170, 591, 1043
Msp20I TGGCCA 1 cut(s) 152
MspA1I CMGCKG 1 cut(s) 257
MspI CCGG 5 cut(s) 339, 437, 504, 680, 832
MspR9I CCNGG 6 cut(s) 36, 56, 357, 367, 584, 832
MunI CAATTG 1 cut(s) 804
MvaI CCWGG 5 cut(s) 36, 56, 357, 367, 584
MvnI CGCG 2 cut(s) 81, 346
MwoI GCNNNNNNNGC 5 cut(s) 39, 144, 376, 827, 934
NciI CCSGG 1 cut(s) 832
NdeII GATC 2 cut(s) 314, 577
NlaIII CATG 4 cut(s) 151, 574, 632, 801
NlaIV GGNNCC 3 cut(s) 33, 316, 566
NmeAIII GCCGAG 1 cut(s) 989
NmuCI GTSAC 2 cut(s) 331, 961
PauI GCGCGC 1 cut(s) 344
PceI AGGCCT 2 cut(s) 42, 59
PcsI WCGNNNNNNNCGW 1 cut(s) 306
PfeI GAWTC 2 cut(s) 541, 739
PkrI GCNGC 6 cut(s) 80, 85, 256, 259, 381, 705
PleI GAGTC 3 cut(s) 370, 542, 1001
PpsI GAGTC 3 cut(s) 370, 542, 1001
Ppu21I YACGTR 2 cut(s) 227, 487
Psp6I CCWGG 5 cut(s) 34, 54, 355, 365, 582
PspGI CCWGG 5 cut(s) 34, 54, 355, 365, 582
PspN4I GGNNCC 3 cut(s) 33, 316, 566
PspOMI GGGCCC 1 cut(s) 31
PspPI GGNCC 6 cut(s) 31, 32, 69, 241, 859, 1030
PstI CTGCAG 1 cut(s) 384
PsuI RGATCY 1 cut(s) 314
PteI GCGCGC 1 cut(s) 344
PvuII CAGCTG 1 cut(s) 257
RsaI GTAC 2 cut(s) 431, 538
RsaNI GTAC 2 cut(s) 430, 537
SalI GTCGAC 1 cut(s) 203
SaqAI TTAA 3 cut(s) 170, 591, 1043
SatI GCNGC 6 cut(s) 79, 84, 255, 258, 380, 704
Sau3AI GATC 2 cut(s) 314, 577
Sau96I GGNCC 6 cut(s) 31, 32, 69, 241, 859, 1030
ScaI AGTACT 1 cut(s) 431
SchI GAGTC 3 cut(s) 371, 542, 1001
ScrFI CCNGG 6 cut(s) 36, 56, 357, 367, 584, 832
SduI GDGCHC 1 cut(s) 35
SfaNI GCATC 2 cut(s) 225, 336
SfcI CTRYAG 1 cut(s) 380
SfiI GGCCNNNNNGGCC 1 cut(s) 39
SgrDI CGTCGACG 1 cut(s) 203
SinI GGWCC 3 cut(s) 241, 859, 1030
SmlI CTYRAG 1 cut(s) 919
SmoI CTYRAG 1 cut(s) 919
Sse9I AATT 1 cut(s) 804
SseBI AGGCCT 2 cut(s) 42, 59
SsiI CCGC 3 cut(s) 79, 89, 271
SspMI CTAG 4 cut(s) 422, 555, 695, 827
StuI AGGCCT 2 cut(s) 42, 59
StyD4I CCNGG 6 cut(s) 34, 54, 355, 365, 582, 830
TaaI ACNGT 3 cut(s) 310, 761, 896
TaiI ACGT 5 cut(s) 144, 229, 393, 489, 591
TaqI TCGA 2 cut(s) 204, 546
TasI AATT 1 cut(s) 804
TatI WGTACW 1 cut(s) 429
TauI GCSGC 1 cut(s) 81
TfiI GAWTC 2 cut(s) 541, 739
Tru1I TTAA 3 cut(s) 170, 591, 1043
Tru9I TTAA 3 cut(s) 170, 591, 1043
TscAI CASTG 5 cut(s) 474, 648, 735, 822, 879
TseFI GTSAC 2 cut(s) 331, 961
TseI GCWGC 5 cut(s) 83, 254, 257, 379, 703
Tsp45I GTSAC 2 cut(s) 331, 961
TspDTI ATGAA 2 cut(s) 17, 786
TspGWI ACGGA 1 cut(s) 280
TspRI CASTG 5 cut(s) 474, 648, 735, 822, 879
VpaK11BI GGWCC 3 cut(s) 241, 859, 1030
XagI CCTNNNNNAGG 2 cut(s) 53, 64
XmiI GTMKAC 2 cut(s) 204, 791
XspI CTAG 4 cut(s) 422, 555, 695, 827
ZrmI AGTACT 1 cut(s) 431
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.