AT4G18180

Glycosyl hydrolases family 28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0014404
Physical Location & Seq
Forward (+)
10065291 .. 10067521
2231 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G18180.1

Sequence Viewer

Length: 1269 bp
ATGTACATCGAGATGGAGAATTTTCGTTACCTGAAATTTTTCTTCTTCTTCATCTTTGTAATATTATCTCCTTCTGAAGCAGGAAATATAGTAGACAAAAGCTCTGTTTTCCTTGTTGATGTCCGTAGCTTTGGTGCTCGAGCTAATGACCACAGAGACCACACCAAGGCCTTTGTAGCAGCATGGGACAAAGCATGCAAATCATCTTCAAGCTCTGTGAATCTTATAATCCCAAGAGGTGAATTCTCTGTTGGTTCTCTCAGATTCTCTGGTCCTTGCACTAATGTATCCAATCTCACTGTCCGAGTTAAGGGTCATCTCAAGGCATCAACTGATCTGTCAAAGTATAGATCAGGCGGTGGTTGGATTCAGTTTGGATGGATCAATGGCTTAACTCTTACCGGAGGTGGAACATTTGACGGTCAAGGCGCTTTAGCTTGGCCGTTTAATAACTGTACTTCTGATTCTAACTGCAAACTCCTTCCAACTAGCTTGAAGTTTGTGGGGATGAACAGAACAGTTGTGAGAAGGATAAGCTCAGTGAATAGCAAGTTCTTTCATATAGCATTAGTAGAATGCAGAGACTTCAAAGGGACAAGACTCAACATCACTGCTCCTTCTGATAGTCCAAATACAGATGGTATACATATCGAACGCAGTTCGAATGTGTATTTCTCGCGTTCACATATCGCAACTGGAGATGATTGTGTCTCGATAGGACAAGGAAATTCTCAGATTACAATCACAAGTATCAAATGTGGACCAGGACATGGTATCAGCGTTGGGAGTTTAGGGAGATATCCAAATGAGAAAGATGTTAATGGACTGGTGGTGAAGGATTGCAAGATAAGCGGTACGACGAATGGGATAAGGATCAAAACTTGGGCTAATTCTCCTGGTCTAAGTGCTGCTACCAACATGACTTTTGAGAATATTATTATGAACAATGTGACTAATCCAATCATCATTGATCAGTCTTATTGTCCTTTCTCTTCTTGCATTTCAAATGTACCTTCAAAGGTTGAGCTGAGTGAGATATACTTCAAGAATATAAGAGGGACATCATCTTCACTGGTCGCGGTGCAGCTGCATTGTAGCAGAGGAATGCCATGTAAAAAAGTGTACTTAGAGAATGTTCATCTTGATCTTTCTTCGTCTGATGGAGGAAGAAAACAGAGTAGTAACAGAGGAAATGAAGCTGTCTCTTCTTCTTGTAGAAACGTTAGAGCTAATTACATTGGGACTCAAATCCCACCTCCATGTCACTAA

Protein Analysis

422

Amino Acids

45.97

Weight (kDa)

9.27

Isoelectric Point (pI)

42.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 70 - 386 1.8e-90 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014404)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G18180
fragaria_vesca FvH4_2g16950
malus_domestica MD05G1141300.v1.1 MD05G1142200.v1.1
prunus_persica Prupe.8G181300_v2.0.a1
pyrus_communis pycom05g13670
rosa_chinensis RchiOBHm_Chr6g0281151
rosa_laevigata RLG00000012990
rosa_multiflora Rmu_co8202118.1_g000001 Rmu_sc0035236.1_g000002
rosa_roxburghii Rroxscaffold_7G00187250
rosa_rugosa Rorug06G0139300
rosa_samantha Rh6AG251100 Rh6BG254300 Rh6CG253700 Rh6DG245700
rosa_wichuraiana Rw6G021800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 227
AccB7I CCANNNNNTGG 1 cut(s) 770
AccI GTMKAC 2 cut(s) 93, 643
AccII CGCG 2 cut(s) 679, 1079
AciI CCGC 3 cut(s) 357, 852, 1079
AclI AACGTT 1 cut(s) 1221
AclWI GGATC 2 cut(s) 389, 881
AcoI YGGCCR 1 cut(s) 440
AcsI RAATTY 4 cut(s) 19, 35, 242, 727
AcuI CTGAAG 1 cut(s) 96
AfaI GTAC 5 cut(s) 5, 457, 856, 1011, 1124
AfiI CCNNNNNNNGG 3 cut(s) 166, 310, 770
AgsI TTSAA 6 cut(s) 210, 496, 589, 1005, 1017, 1045
AjnI CCWGG 2 cut(s) 763, 895
AjuI GAANNNNNNNTTGG 2 cut(s) 234, 266
Alw21I GWGCWC 1 cut(s) 139
Alw26I GTCTC 4 cut(s) 150, 576, 715, 1207
AlwI GGATC 2 cut(s) 389, 881
Ama87I CYCGRG 1 cut(s) 138
AoxI GGCC 2 cut(s) 168, 440
ApeKI GCWGC 4 cut(s) 179, 908, 1084, 1087
ApoI RAATTY 4 cut(s) 19, 35, 242, 727
Asp700I GAANNNNTTC 1 cut(s) 38
AspLEI GCGC 1 cut(s) 431
AspS9I GGNCC 2 cut(s) 272, 761
AsuHPI GGTGA 2 cut(s) 251, 844
AsuII TTCGAA 1 cut(s) 662
AvaI CYCGRG 1 cut(s) 138
AvaII GGWCC 2 cut(s) 272, 761
BaeI ACNNNNGTAYC 2 cut(s) 270, 303
Bbv12I GWGCWC 1 cut(s) 139
BbvI GCAGC 4 cut(s) 191, 895, 1074, 1096
BccI CCATC 4 cut(s) 7, 372, 632, 1154
BceAI ACGGC 1 cut(s) 427
BciT130I CCWGG 2 cut(s) 765, 897
BciVI GTATCC 1 cut(s) 298
BclI TGATCA 1 cut(s) 970
BcoDI GTCTC 4 cut(s) 150, 576, 715, 1207
BfaI CTAG 1 cut(s) 489
BfoI RGCGCY 1 cut(s) 432
BfuI GTATCC 1 cut(s) 298
BisI GCNGC 4 cut(s) 180, 909, 1085, 1088
BlsI GCNGC 4 cut(s) 181, 910, 1086, 1089
Bme1390I CCNGG 2 cut(s) 765, 897
Bme18I GGWCC 2 cut(s) 272, 761
BmeT110I CYCGRG 1 cut(s) 138
BmgT120I GGNCC 2 cut(s) 272, 761
BmrFI CCNGG 2 cut(s) 765, 897
BmsI GCATC 1 cut(s) 335
BpmI CTGGAG 1 cut(s) 717
Bpu14I TTCGAA 1 cut(s) 662
BpuEI CTTGAG 1 cut(s) 305
BsaBI GATNNNNATC 2 cut(s) 740, 872
BsaI GGTCTC 1 cut(s) 150
BsaJI CCNNGG 1 cut(s) 165
BsaWI WCCGGW 1 cut(s) 401
Bsc4I CCNNNNNNNGG 3 cut(s) 166, 310, 770
Bse1I ACTGG 3 cut(s) 700, 831, 1077
Bse8I GATNNNNATC 2 cut(s) 740, 872
BseBI CCWGG 2 cut(s) 765, 897
BseDI CCNNGG 1 cut(s) 165
BseGI GGATG 2 cut(s) 383, 513
BseJI GATNNNNATC 2 cut(s) 740, 872
BseLI CCNNNNNNNGG 3 cut(s) 166, 310, 770
BseMII CTCAG 4 cut(s) 274, 552, 746, 1019
BseNI ACTGG 3 cut(s) 700, 831, 1077
BseXI GCAGC 4 cut(s) 191, 895, 1074, 1096
BsgI GTGCAG 1 cut(s) 1103
Bsh1236I CGCG 2 cut(s) 679, 1079
BshFI GGCC 2 cut(s) 170, 442
BsiHKAI GWGCWC 1 cut(s) 139
BsiHKCI CYCGRG 1 cut(s) 138
BsiSI CCGG 1 cut(s) 402
BslFI GGGAC 4 cut(s) 200, 607, 1072, 1255
BslI CCNNNNNNNGG 3 cut(s) 166, 310, 770
BsmAI GTCTC 4 cut(s) 150, 576, 715, 1207
BsmFI GGGAC 4 cut(s) 200, 607, 1072, 1255
BsmI GAATGC 2 cut(s) 581, 1110
BsnI GGCC 2 cut(s) 170, 442
Bso31I GGTCTC 1 cut(s) 150
BsoBI CYCGRG 1 cut(s) 138
Bsp119I TTCGAA 1 cut(s) 662
Bsp1286I GDGCHC 1 cut(s) 139
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 6 cut(s) 334, 350, 381, 873, 970, 1144
BspACI CCGC 3 cut(s) 357, 852, 1079
BspANI GGCC 2 cut(s) 170, 442
BspCNI CTCAG 4 cut(s) 273, 551, 745, 1020
BspFNI CGCG 2 cut(s) 679, 1079
BspPI GGATC 2 cut(s) 389, 881
BspT104I TTCGAA 1 cut(s) 662
BspTNI GGTCTC 1 cut(s) 150
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 3 cut(s) 700, 831, 1077
BssECI CCNNGG 1 cut(s) 165
BssMI GATC 6 cut(s) 334, 350, 381, 873, 970, 1144
BssNAI GTATAC 1 cut(s) 644
BssT1I CCWWGG 1 cut(s) 165
Bst1107I GTATAC 1 cut(s) 644
Bst2UI CCWGG 2 cut(s) 765, 897
Bst4CI ACNGT 4 cut(s) 301, 422, 455, 520
Bst6I CTCTTC 2 cut(s) 997, 1210
BstAUI TGTACA 1 cut(s) 3
BstBI TTCGAA 1 cut(s) 662
BstC8I GCNNGC 1 cut(s) 196
BstDEI CTNAG 6 cut(s) 260, 538, 732, 902, 1028, 1126
BstF5I GGATG 2 cut(s) 383, 513
BstFNI CGCG 2 cut(s) 679, 1079
BstH2I RGCGCY 1 cut(s) 432
BstHHI GCGC 1 cut(s) 431
BstKTI GATC 6 cut(s) 337, 353, 384, 876, 973, 1147
BstMAI GTCTC 4 cut(s) 150, 576, 715, 1207
BstMBI GATC 6 cut(s) 334, 350, 381, 873, 970, 1144
BstMWI GCNNNNNNNGC 2 cut(s) 176, 849
BstNI CCWGG 2 cut(s) 765, 897
BstNSI RCATGY 1 cut(s) 198
BstSCI CCNGG 2 cut(s) 763, 895
BstUI CGCG 2 cut(s) 679, 1079
BstV1I GCAGC 4 cut(s) 191, 895, 1074, 1096
BstZ17I GTATAC 1 cut(s) 644
BsuI GTATCC 1 cut(s) 298
BsuRI GGCC 2 cut(s) 170, 442
BtsCI GGATG 2 cut(s) 383, 513
BtsI GCAGTG 1 cut(s) 609
BtsIMutI CAGTG 4 cut(s) 297, 546, 609, 1070
Cac8I GCNNGC 1 cut(s) 196
CfoI GCGC 1 cut(s) 431
Cfr13I GGNCC 2 cut(s) 272, 761
Csp6I GTAC 5 cut(s) 4, 456, 855, 1010, 1123
CviAII CATG 6 cut(s) 183, 195, 770, 919, 1110, 1260
CviQI GTAC 5 cut(s) 4, 456, 855, 1010, 1123
DdeI CTNAG 6 cut(s) 260, 538, 732, 902, 1028, 1126
DpnI GATC 6 cut(s) 336, 352, 383, 875, 972, 1146
DpnII GATC 6 cut(s) 334, 350, 381, 873, 970, 1144
EaeI YGGCCR 1 cut(s) 440
Eam1104I CTCTTC 2 cut(s) 997, 1210
EarI CTCTTC 2 cut(s) 997, 1210
Eco130I CCWWGG 1 cut(s) 165
Eco147I AGGCCT 1 cut(s) 170
Eco31I GGTCTC 1 cut(s) 150
Eco32I GATATC 1 cut(s) 800
Eco47I GGWCC 2 cut(s) 272, 761
Eco57I CTGAAG 1 cut(s) 96
Eco88I CYCGRG 1 cut(s) 138
EcoRI GAATTC 1 cut(s) 242
EcoRII CCWGG 2 cut(s) 763, 895
EcoRV GATATC 1 cut(s) 800
EcoT14I CCWWGG 1 cut(s) 165
ErhI CCWWGG 1 cut(s) 165
FaeI CATG 6 cut(s) 186, 198, 773, 922, 1113, 1263
FaqI GGGAC 4 cut(s) 200, 607, 1072, 1255
FatI CATG 6 cut(s) 182, 194, 769, 918, 1109, 1259
FbaI TGATCA 1 cut(s) 970
FblI GTMKAC 2 cut(s) 93, 643
Fnu4HI GCNGC 4 cut(s) 180, 909, 1085, 1088
FokI GGATG 2 cut(s) 390, 520
Fsp4HI GCNGC 4 cut(s) 180, 909, 1085, 1088
FspBI CTAG 1 cut(s) 489
GlaI GCGC 1 cut(s) 430
GluI GCNGC 4 cut(s) 180, 909, 1085, 1088
GsuI CTGGAG 1 cut(s) 717
HaeII RGCGCY 1 cut(s) 432
HaeIII GGCC 2 cut(s) 170, 442
HapII CCGG 1 cut(s) 402
HhaI GCGC 1 cut(s) 431
Hin1II CATG 6 cut(s) 186, 198, 773, 922, 1113, 1263
Hin6I GCGC 1 cut(s) 429
HinP1I GCGC 1 cut(s) 429
HinfI GANTC 6 cut(s) 220, 264, 367, 464, 600, 1243
HpaII CCGG 1 cut(s) 402
HphI GGTGA 2 cut(s) 251, 844
Hpy166II GTNNAC 5 cut(s) 94, 644, 683, 761, 1123
Hpy188I TCNGA 7 cut(s) 76, 263, 305, 463, 622, 735, 1159
Hpy188III TCNNGA 4 cut(s) 10, 712, 1045, 1142
Hpy8I GTNNAC 5 cut(s) 94, 644, 683, 761, 1123
Hpy99I CGWCG 1 cut(s) 862
HpyAV CCTTC 6 cut(s) 81, 491, 522, 627, 829, 1023
HpyCH4III ACNGT 4 cut(s) 301, 422, 455, 520
HpyCH4IV ACGT 1 cut(s) 1221
HpyCH4V TGCA 8 cut(s) 198, 279, 474, 579, 843, 999, 1084, 1090
HpyF10VI GCNNNNNNNGC 2 cut(s) 176, 849
HpyF3I CTNAG 6 cut(s) 260, 538, 732, 902, 1028, 1126
HpySE526I ACGT 1 cut(s) 1221
Hsp92II CATG 6 cut(s) 186, 198, 773, 922, 1113, 1263
HspAI GCGC 1 cut(s) 429
Ksp22I TGATCA 1 cut(s) 970
Kzo9I GATC 6 cut(s) 334, 350, 381, 873, 970, 1144
LmnI GCTCC 1 cut(s) 619
Lsp1109I GCAGC 4 cut(s) 191, 895, 1074, 1096
LweI GCATC 1 cut(s) 335
MaeI CTAG 1 cut(s) 489
MaeII ACGT 1 cut(s) 1221
MaeIII GTNAC 4 cut(s) 26, 949, 1181, 1262
MalI GATC 6 cut(s) 336, 352, 383, 875, 972, 1146
MboI GATC 6 cut(s) 334, 350, 381, 873, 970, 1144
MhlI GDGCHC 1 cut(s) 139
MluCI AATT 6 cut(s) 19, 35, 242, 727, 889, 1231
MlyI GAGTC 2 cut(s) 594, 1237
MmeI TCCRAC 2 cut(s) 344, 509
MnlI CCTC 7 cut(s) 230, 398, 1049, 1094, 1157, 1181, 1266
MroXI GAANNNNTTC 1 cut(s) 38
MseI TTAA 4 cut(s) 309, 392, 447, 819
MslI CAYNNNNRTG 2 cut(s) 11, 1258
MspA1I CMGCKG 1 cut(s) 1087
MspI CCGG 1 cut(s) 402
MspR9I CCNGG 2 cut(s) 765, 897
Mva1269I GAATGC 2 cut(s) 581, 1110
MvaI CCWGG 2 cut(s) 765, 897
MvnI CGCG 2 cut(s) 679, 1079
MwoI GCNNNNNNNGC 2 cut(s) 176, 849
NdeII GATC 6 cut(s) 334, 350, 381, 873, 970, 1144
NlaIII CATG 6 cut(s) 186, 198, 773, 922, 1113, 1263
NmuCI GTSAC 2 cut(s) 949, 1262
NspI RCATGY 1 cut(s) 198
NspV TTCGAA 1 cut(s) 662
PaeI GCATGC 1 cut(s) 198
PaeR7I CTCGAG 1 cut(s) 138
PceI AGGCCT 1 cut(s) 170
PctI GAATGC 2 cut(s) 581, 1110
PdmI GAANNNNTTC 1 cut(s) 38
PfeI GAWTC 4 cut(s) 220, 264, 367, 464
PflMI CCANNNNNTGG 1 cut(s) 770
PkrI GCNGC 4 cut(s) 181, 910, 1086, 1089
PleI GAGTC 2 cut(s) 594, 1237
PpsI GAGTC 2 cut(s) 594, 1237
PsiI TTATAA 1 cut(s) 227
Psp1406I AACGTT 1 cut(s) 1221
Psp6I CCWGG 2 cut(s) 763, 895
PspGI CCWGG 2 cut(s) 763, 895
PspPI GGNCC 2 cut(s) 272, 761
PspXI VCTCGAGB 1 cut(s) 138
PvuII CAGCTG 1 cut(s) 1087
RsaI GTAC 5 cut(s) 5, 457, 856, 1011, 1124
RsaNI GTAC 5 cut(s) 4, 456, 855, 1010, 1123
RseI CAYNNNNRTG 2 cut(s) 11, 1258
SaqAI TTAA 4 cut(s) 309, 392, 447, 819
SatI GCNGC 4 cut(s) 180, 909, 1085, 1088
Sau3AI GATC 6 cut(s) 334, 350, 381, 873, 970, 1144
Sau96I GGNCC 2 cut(s) 272, 761
SchI GAGTC 2 cut(s) 594, 1237
ScrFI CCNGG 2 cut(s) 765, 897
SduI GDGCHC 1 cut(s) 139
SfaNI GCATC 1 cut(s) 335
Sfr274I CTCGAG 1 cut(s) 138
SfuI TTCGAA 1 cut(s) 662
SinI GGWCC 2 cut(s) 272, 761
SlaI CTCGAG 1 cut(s) 138
SmiMI CAYNNNNRTG 2 cut(s) 11, 1258
SmlI CTYRAG 2 cut(s) 138, 320
SmoI CTYRAG 2 cut(s) 138, 320
SphI GCATGC 1 cut(s) 198
Sse9I AATT 6 cut(s) 19, 35, 242, 727, 889, 1231
SseBI AGGCCT 1 cut(s) 170
SsiI CCGC 3 cut(s) 357, 852, 1079
SspI AATATT 2 cut(s) 63, 934
SspMI CTAG 1 cut(s) 489
StuI AGGCCT 1 cut(s) 170
StyD4I CCNGG 2 cut(s) 763, 895
StyI CCWWGG 1 cut(s) 165
TaaI ACNGT 4 cut(s) 301, 422, 455, 520
TaiI ACGT 1 cut(s) 1224
TaqI TCGA 5 cut(s) 9, 139, 651, 662, 713
TasI AATT 6 cut(s) 19, 35, 242, 727, 889, 1231
TatI WGTACW 3 cut(s) 3, 455, 1122
TfiI GAWTC 4 cut(s) 220, 264, 367, 464
Tru1I TTAA 4 cut(s) 309, 392, 447, 819
Tru9I TTAA 4 cut(s) 309, 392, 447, 819
TscAI CASTG 4 cut(s) 304, 546, 616, 1077
TseFI GTSAC 2 cut(s) 949, 1262
TseI GCWGC 4 cut(s) 179, 908, 1084, 1087
Tsp45I GTSAC 2 cut(s) 949, 1262
TspDTI ATGAA 6 cut(s) 40, 524, 548, 956, 1127, 1209
TspGWI ACGGA 1 cut(s) 113
TspRI CASTG 4 cut(s) 304, 546, 616, 1077
Van91I CCANNNNNTGG 1 cut(s) 770
VpaK11BI GGWCC 2 cut(s) 272, 761
XapI RAATTY 4 cut(s) 19, 35, 242, 727
XceI RCATGY 1 cut(s) 198
XhoI CTCGAG 1 cut(s) 138
XmiI GTMKAC 2 cut(s) 93, 643
XmnI GAANNNNTTC 1 cut(s) 38
XspI CTAG 1 cut(s) 489
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.