Rw6G021800

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0014404
Physical Location & Seq
Reverse (-)
43359690 .. 43361649
1960 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G021800.1

Sequence Viewer

Length: 1263 bp
ATGAGCCTCGACTACTTCTATGTTAGATTGCTCTTATTGCTGTCCTTATTTTGCTTCGAATGTGTGTTTGGCAGAGAAAGAGGAGCAGGTTCAGGCTTGCAGACCATCAATGTAGATCGCAAGGCCAAAAGCTCTCTTCTTTTTAATGTCAAGAGCTTTGGTGCCAAAGCAGATGGCCAAACCGATGACAGCAAAGCCTTCACAGCAGCATGGAAAGAGGCGTGCCAGGCCAAAGGTAGAGTCCACTTAATAATCCCAAGAGGAACATACATGATTGGCCCTCTTAAGTTTTCAGGCCCCTGCCAAAACGTATCGGCTCTAAATCTTCGAGTAAAGGGTTACTTGAAGGCTACAGCGGAGTTGTCCAAGTATGAATTCGGTGGCGGTTGGATTGAATTTGCTTGGATGAAGGGGCTAACCTTGACCGGAGGCGGAACCTTTGATGGTCAAGGTGCTAAAGCCTGGCCTCATAATAAGTGCTCTAAAGACTCCAATTGCAAACTTCTTCCAACTAGTTTGAAATTTGTTGCAATGAATAAGACAGTGATCCGGGGCATATCCTCGGTGAACAGCAAGTTCTTTCATATAGCTGTTGTGGAATGTCACAACTTCAAAGGTAGTAAACTCAAGATTCAAGCCCCAGAAACTAGTCCTAATACTGATGGAATCCACATTGAACGAAGCTCGAGTGTGTACTTCTCTTCGTCACACATTGCTACTGGTGATGACTGCATCTCAGTTGGACAAGGGAACTCCCAAGTCACCATAACTAGCATTGCTTGTGGACCTGGTCATGGCATCAGTGTGGGAAGTTTGGGAAAATATCCGAATGAAGGAGATGTTAGTGGGCTTGTGGTCAGAGACTGCACCATTTCAGGTACCACAAATGGTATTAGGATCAAGACATGGGCTAACTCTCCAGGTAGAAGTGCAGCCACCAACATGACATTCACAAACATCATCATGAACAATGTGACCAACCCCATCATAATCGATCAGACATATTGTCCATTCACGTCTTGCGCTACTAAGGTGCCATCAAAAGTGAAGCTGAGTGACATTTACTTCAAGAACATAAGAGGGACCTCGTCGTCTCAAGTAGCAGTTGCACTTGAATGTAGCAAAGGGATTCCATGCCAGAACATTTACCTTGAAGATGTTCATCTGAACTTGTCATCTGGGAAGAAGGAAGCCACTTCAACTTGCAGTAATGTTAAAGCCAAATTCATTGGCACCCAAATCCCACCACCATGTACTGATTAG

Protein Analysis

420

Amino Acids

45.14

Weight (kDa)

9.21

Isoelectric Point (pI)

34.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pect-lyase_RHGA_epim PF12708 48 - 92 1.2e-06 Rhamnogalacturonase A/epimerase, pectate lyase-like
Glyco_hydro_28 PF00295 75 - 408 2.8e-93 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014404)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G18180
fragaria_vesca FvH4_2g16950
malus_domestica MD05G1141300.v1.1 MD05G1142200.v1.1
prunus_persica Prupe.8G181300_v2.0.a1
pyrus_communis pycom05g13670
rosa_chinensis RchiOBHm_Chr6g0281151
rosa_laevigata RLG00000012990
rosa_multiflora Rmu_co8202118.1_g000001 Rmu_sc0035236.1_g000002
rosa_roxburghii Rroxscaffold_7G00187250
rosa_rugosa Rorug06G0139300
rosa_samantha Rh6AG251100 Rh6BG254300 Rh6CG253700 Rh6DG245700
rosa_wichuraiana Rw6G021800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1090
Acc36I ACCTGC 1 cut(s) 77
Acc65I GGTACC 1 cut(s) 878
AccB1I GGYRCC 4 cut(s) 161, 878, 1033, 1232
AciI CCGC 3 cut(s) 356, 384, 432
AclWI GGATC 2 cut(s) 541, 905
AcoI YGGCCR 1 cut(s) 175
AcsI RAATTY 4 cut(s) 374, 395, 521, 1223
AfaI GTAC 3 cut(s) 695, 880, 1255
AfiI CCNNNNNNNGG 2 cut(s) 794, 833
AflII CTTAAG 1 cut(s) 284
AhdI GACNNNNNGTC 1 cut(s) 1005
AhlI ACTAGT 2 cut(s) 512, 647
AjiI CACGTC 1 cut(s) 1017
AjnI CCWGG 4 cut(s) 225, 461, 787, 919
AjuI GAANNNNNNNTTGG 6 cut(s) 51, 83, 359, 391, 932, 964
AluBI AGCT 5 cut(s) 132, 156, 590, 684, 1051
AluI AGCT 5 cut(s) 132, 156, 590, 684, 1051
Alw21I GWGCWC 1 cut(s) 482
Alw26I GTCTC 2 cut(s) 855, 1098
AlwI GGATC 2 cut(s) 541, 905
AlwNI CAGNNNCTG 1 cut(s) 864
Ama87I CYCGRG 1 cut(s) 685
AoxI GGCC 6 cut(s) 123, 175, 228, 277, 295, 464
ApeKI GCWGC 2 cut(s) 206, 932
ApoI RAATTY 4 cut(s) 374, 395, 521, 1223
ArsI GACNNNNNNTTYG 2 cut(s) 225, 257
Asp700I GAANNNNTTC 1 cut(s) 1158
Asp718I GGTACC 1 cut(s) 878
AspLEI GCGC 1 cut(s) 1025
AspS9I GGNCC 4 cut(s) 278, 296, 785, 1083
AsuC2I CCSGG 1 cut(s) 551
AsuHPI GGTGA 3 cut(s) 577, 734, 754
AsuII TTCGAA 1 cut(s) 57
AvaI CYCGRG 1 cut(s) 685
AvaII GGWCC 2 cut(s) 785, 1083
BalI TGGCCA 1 cut(s) 177
BanI GGYRCC 4 cut(s) 161, 878, 1033, 1232
Bbv12I GWGCWC 1 cut(s) 482
BbvI GCAGC 2 cut(s) 218, 944
BccI CCATC 6 cut(s) 113, 167, 437, 656, 992, 1045
BciT130I CCWGG 4 cut(s) 227, 463, 789, 921
BcnI CCSGG 1 cut(s) 551
BcoDI GTCTC 2 cut(s) 855, 1098
BcuI ACTAGT 2 cut(s) 512, 647
BfaI CTAG 3 cut(s) 513, 648, 771
BfmI CTRYAG 1 cut(s) 351
BfrI CTTAAG 1 cut(s) 284
BfuAI ACCTGC 1 cut(s) 77
BisI GCNGC 2 cut(s) 207, 933
BlsI GCNGC 2 cut(s) 208, 934
Bme1390I CCNGG 5 cut(s) 227, 463, 551, 789, 921
Bme18I GGWCC 2 cut(s) 785, 1083
BmeRI GACNNNNNGTC 1 cut(s) 1005
BmeT110I CYCGRG 1 cut(s) 685
BmgBI CACGTC 1 cut(s) 1017
BmgT120I GGNCC 4 cut(s) 278, 296, 785, 1083
BmiI GGNNCC 7 cut(s) 163, 298, 436, 880, 1035, 1084, 1234
BmrFI CCNGG 5 cut(s) 227, 463, 551, 789, 921
BmsI GCATC 2 cut(s) 741, 807
BpmI CTGGAG 1 cut(s) 903
Bpu14I TTCGAA 1 cut(s) 57
BpuEI CTTGAG 2 cut(s) 611, 1080
BpuMI CCSGG 1 cut(s) 551
Bsa29I ATCGAT 1 cut(s) 993
BsaBI GATNNNNATC 1 cut(s) 1161
BsaJI CCNNGG 2 cut(s) 550, 561
BsaWI WCCGGW 1 cut(s) 425
Bsc4I CCNNNNNNNGG 2 cut(s) 794, 833
Bse1I ACTGG 1 cut(s) 724
Bse3DI GCAATG 3 cut(s) 537, 711, 774
Bse8I GATNNNNATC 1 cut(s) 1161
BseBI CCWGG 4 cut(s) 227, 463, 789, 921
BseCI ATCGAT 1 cut(s) 993
BseDI CCNNGG 2 cut(s) 550, 561
BseGI GGATG 1 cut(s) 411
BseJI GATNNNNATC 1 cut(s) 1161
BseLI CCNNNNNNNGG 2 cut(s) 794, 833
BseMI GCAATG 3 cut(s) 537, 711, 774
BseMII CTCAG 2 cut(s) 750, 1043
BseNI ACTGG 1 cut(s) 724
BseRI GAGGAG 1 cut(s) 96
BseXI GCAGC 2 cut(s) 218, 944
BsgI GTGCAG 2 cut(s) 850, 951
BshFI GGCC 6 cut(s) 125, 177, 230, 279, 297, 466
BshNI GGYRCC 4 cut(s) 161, 878, 1033, 1232
BshVI ATCGAT 1 cut(s) 993
BsiHKAI GWGCWC 1 cut(s) 482
BsiHKCI CYCGRG 1 cut(s) 685
BsiSI CCGG 2 cut(s) 426, 550
BslFI GGGAC 1 cut(s) 1096
BslI CCNNNNNNNGG 2 cut(s) 794, 833
BsmAI GTCTC 2 cut(s) 855, 1098
BsmBI CGTCTC 1 cut(s) 1098
BsmFI GGGAC 1 cut(s) 1096
BsnI GGCC 6 cut(s) 125, 177, 230, 279, 297, 466
BsoBI CYCGRG 1 cut(s) 685
Bsp119I TTCGAA 1 cut(s) 57
Bsp1286I GDGCHC 1 cut(s) 482
Bsp143I GATC 4 cut(s) 115, 546, 897, 994
BspACI CCGC 3 cut(s) 356, 384, 432
BspANI GGCC 6 cut(s) 125, 177, 230, 279, 297, 466
BspCNI CTCAG 2 cut(s) 749, 1044
BspDI ATCGAT 1 cut(s) 993
BspHI TCATGA 1 cut(s) 963
BspLI GGNNCC 7 cut(s) 163, 298, 436, 880, 1035, 1084, 1234
BspMI ACCTGC 1 cut(s) 77
BspPI GGATC 2 cut(s) 541, 905
BspT104I TTCGAA 1 cut(s) 57
BspT107I GGYRCC 4 cut(s) 161, 878, 1033, 1232
BspTI CTTAAG 1 cut(s) 284
BsrDI GCAATG 3 cut(s) 537, 711, 774
BsrI ACTGG 1 cut(s) 724
BssECI CCNNGG 2 cut(s) 550, 561
BssMI GATC 4 cut(s) 115, 546, 897, 994
Bst2UI CCWGG 4 cut(s) 227, 463, 789, 921
Bst4CI ACNGT 1 cut(s) 544
Bst6I CTCTTC 2 cut(s) 141, 706
BstAFI CTTAAG 1 cut(s) 284
BstBI TTCGAA 1 cut(s) 57
BstC8I GCNNGC 2 cut(s) 98, 223
BstDEI CTNAG 3 cut(s) 736, 1029, 1052
BstF5I GGATG 1 cut(s) 411
BstHHI GCGC 1 cut(s) 1025
BstKTI GATC 4 cut(s) 118, 549, 900, 997
BstMAI GTCTC 2 cut(s) 855, 1098
BstMBI GATC 4 cut(s) 115, 546, 897, 994
BstMWI GCNNNNNNNGC 3 cut(s) 37, 203, 227
BstNI CCWGG 4 cut(s) 227, 463, 789, 921
BstSCI CCNGG 5 cut(s) 225, 461, 549, 787, 919
BstSFI CTRYAG 1 cut(s) 351
BstV1I GCAGC 2 cut(s) 218, 944
Bsu15I ATCGAT 1 cut(s) 993
BsuRI GGCC 6 cut(s) 125, 177, 230, 279, 297, 466
BsuTUI ATCGAT 1 cut(s) 993
BtrI CACGTC 1 cut(s) 1017
BtsCI GGATG 1 cut(s) 411
BtsIMutI CAGTG 2 cut(s) 549, 808
BveI ACCTGC 1 cut(s) 77
Cac8I GCNNGC 2 cut(s) 98, 223
CaiI CAGNNNCTG 1 cut(s) 864
CciI TCATGA 1 cut(s) 963
CfoI GCGC 1 cut(s) 1025
Cfr13I GGNCC 4 cut(s) 278, 296, 785, 1083
ClaI ATCGAT 1 cut(s) 993
CsiI ACCWGGT 1 cut(s) 787
Csp6I GTAC 3 cut(s) 694, 879, 1254
CviAII CATG 8 cut(s) 210, 271, 794, 906, 943, 964, 1134, 1251
CviQI GTAC 3 cut(s) 694, 879, 1254
DdeI CTNAG 3 cut(s) 736, 1029, 1052
DpnI GATC 4 cut(s) 117, 548, 899, 996
DpnII GATC 4 cut(s) 115, 546, 897, 994
DrdI GACNNNNNNGTC 1 cut(s) 1090
DriI GACNNNNNGTC 1 cut(s) 1005
DseDI GACNNNNNNGTC 1 cut(s) 1090
EaeI YGGCCR 1 cut(s) 175
Eam1104I CTCTTC 2 cut(s) 141, 706
Eam1105I GACNNNNNGTC 1 cut(s) 1005
EarI CTCTTC 2 cut(s) 141, 706
EciI GGCGGA 1 cut(s) 447
Eco47I GGWCC 2 cut(s) 785, 1083
Eco88I CYCGRG 1 cut(s) 685
EcoO109I RGGNCCY 2 cut(s) 296, 1083
EcoRI GAATTC 1 cut(s) 374
EcoRII CCWGG 4 cut(s) 225, 461, 787, 919
Esp3I CGTCTC 1 cut(s) 1098
FaeI CATG 8 cut(s) 213, 274, 797, 909, 946, 967, 1137, 1254
FalI AAGNNNNNCTT 2 cut(s) 326, 358
FaqI GGGAC 1 cut(s) 1096
FatI CATG 8 cut(s) 209, 270, 793, 905, 942, 963, 1133, 1250
Fnu4HI GCNGC 2 cut(s) 207, 933
FokI GGATG 1 cut(s) 418
Fsp4HI GCNGC 2 cut(s) 207, 933
FspBI CTAG 3 cut(s) 513, 648, 771
GlaI GCGC 1 cut(s) 1024
GluI GCNGC 2 cut(s) 207, 933
GsuI CTGGAG 1 cut(s) 903
HaeIII GGCC 6 cut(s) 125, 177, 230, 279, 297, 466
HapII CCGG 2 cut(s) 426, 550
HhaI GCGC 1 cut(s) 1025
Hin1II CATG 8 cut(s) 213, 274, 797, 909, 946, 967, 1137, 1254
Hin6I GCGC 1 cut(s) 1023
HinP1I GCGC 1 cut(s) 1023
HinfI GANTC 5 cut(s) 240, 488, 631, 666, 1129
HpaII CCGG 2 cut(s) 426, 550
HphI GGTGA 3 cut(s) 577, 734, 754
Hpy166II GTNNAC 5 cut(s) 244, 568, 623, 694, 785
Hpy188I TCNGA 4 cut(s) 828, 860, 999, 1167
Hpy188III TCNNGA 5 cut(s) 151, 628, 901, 964, 1069
Hpy8I GTNNAC 5 cut(s) 244, 568, 623, 694, 785
Hpy99I CGWCG 1 cut(s) 1093
HpyAV CCTTC 5 cut(s) 208, 340, 403, 827, 1180
HpyCH4III ACNGT 1 cut(s) 544
HpyCH4IV ACGT 2 cut(s) 309, 1016
HpyCH4V TGCA 8 cut(s) 100, 498, 530, 732, 867, 932, 1109, 1206
HpyF10VI GCNNNNNNNGC 3 cut(s) 37, 203, 227
HpyF3I CTNAG 3 cut(s) 736, 1029, 1052
HpySE526I ACGT 2 cut(s) 309, 1016
Hsp92II CATG 8 cut(s) 213, 274, 797, 909, 946, 967, 1137, 1254
HspAI GCGC 1 cut(s) 1023
KpnI GGTACC 1 cut(s) 882
Kzo9I GATC 4 cut(s) 115, 546, 897, 994
LmnI GCTCC 1 cut(s) 83
Lsp1109I GCAGC 2 cut(s) 218, 944
LweI GCATC 2 cut(s) 741, 807
MabI ACCWGGT 1 cut(s) 787
MaeI CTAG 3 cut(s) 513, 648, 771
MaeII ACGT 2 cut(s) 309, 1016
MaeIII GTNAC 6 cut(s) 338, 602, 705, 760, 973, 1055
MalI GATC 4 cut(s) 117, 548, 899, 996
MboI GATC 4 cut(s) 115, 546, 897, 994
MboII GAAGA 6 cut(s) 128, 317, 497, 693, 1166, 1195
MfeI CAATTG 1 cut(s) 493
MhlI GDGCHC 1 cut(s) 482
MlsI TGGCCA 1 cut(s) 177
MluCI AATT 5 cut(s) 374, 395, 493, 521, 1223
MluNI TGGCCA 1 cut(s) 177
MlyI GAGTC 2 cut(s) 249, 482
MmeI TCCRAC 3 cut(s) 368, 533, 721
Mox20I TGGCCA 1 cut(s) 177
MroXI GAANNNNTTC 1 cut(s) 1158
MscI TGGCCA 1 cut(s) 177
MseI TTAA 4 cut(s) 144, 248, 285, 1215
MslI CAYNNNNRTG 5 cut(s) 803, 941, 962, 1114, 1249
Msp20I TGGCCA 1 cut(s) 177
MspA1I CMGCKG 1 cut(s) 356
MspCI CTTAAG 1 cut(s) 284
MspI CCGG 2 cut(s) 426, 550
MspR9I CCNGG 5 cut(s) 227, 463, 551, 789, 921
MunI CAATTG 1 cut(s) 493
MvaI CCWGG 4 cut(s) 227, 463, 789, 921
MwoI GCNNNNNNNGC 3 cut(s) 37, 203, 227
NciI CCSGG 1 cut(s) 551
NdeII GATC 4 cut(s) 115, 546, 897, 994
NlaIII CATG 8 cut(s) 213, 274, 797, 909, 946, 967, 1137, 1254
NlaIV GGNNCC 7 cut(s) 163, 298, 436, 880, 1035, 1084, 1234
NmuCI GTSAC 5 cut(s) 602, 705, 760, 973, 1055
NspV TTCGAA 1 cut(s) 57
PaeR7I CTCGAG 1 cut(s) 685
PagI TCATGA 1 cut(s) 963
PdmI GAANNNNTTC 1 cut(s) 1158
PfeI GAWTC 3 cut(s) 631, 666, 1129
PflFI GACNNNGTC 2 cut(s) 789, 1087
PkrI GCNGC 2 cut(s) 208, 934
PleI GAGTC 2 cut(s) 248, 482
PpsI GAGTC 2 cut(s) 248, 482
PpuMI RGGWCCY 1 cut(s) 1083
Psp5II RGGWCCY 1 cut(s) 1083
Psp6I CCWGG 4 cut(s) 225, 461, 787, 919
PspGI CCWGG 4 cut(s) 225, 461, 787, 919
PspN4I GGNNCC 7 cut(s) 163, 298, 436, 880, 1035, 1084, 1234
PspPI GGNCC 4 cut(s) 278, 296, 785, 1083
PspPPI RGGWCCY 1 cut(s) 1083
PspXI VCTCGAGB 1 cut(s) 685
PstNI CAGNNNCTG 1 cut(s) 864
PsyI GACNNNGTC 2 cut(s) 789, 1087
RsaI GTAC 3 cut(s) 695, 880, 1255
RsaNI GTAC 3 cut(s) 694, 879, 1254
RseI CAYNNNNRTG 5 cut(s) 803, 941, 962, 1114, 1249
SaqAI TTAA 4 cut(s) 144, 248, 285, 1215
SatI GCNGC 2 cut(s) 207, 933
Sau3AI GATC 4 cut(s) 115, 546, 897, 994
Sau96I GGNCC 4 cut(s) 278, 296, 785, 1083
SchI GAGTC 2 cut(s) 249, 482
ScrFI CCNGG 5 cut(s) 227, 463, 551, 789, 921
SduI GDGCHC 1 cut(s) 482
SexAI ACCWGGT 1 cut(s) 787
SfaNI GCATC 2 cut(s) 741, 807
SfcI CTRYAG 1 cut(s) 351
Sfr274I CTCGAG 1 cut(s) 685
SfuI TTCGAA 1 cut(s) 57
SinI GGWCC 2 cut(s) 785, 1083
SlaI CTCGAG 1 cut(s) 685
SmiMI CAYNNNNRTG 5 cut(s) 803, 941, 962, 1114, 1249
SmlI CTYRAG 4 cut(s) 284, 626, 685, 1095
SmoI CTYRAG 4 cut(s) 284, 626, 685, 1095
SpeI ACTAGT 2 cut(s) 512, 647
Sse9I AATT 5 cut(s) 374, 395, 493, 521, 1223
SsiI CCGC 3 cut(s) 356, 384, 432
SspMI CTAG 3 cut(s) 513, 648, 771
StyD4I CCNGG 5 cut(s) 225, 461, 549, 787, 919
TaaI ACNGT 1 cut(s) 544
TaiI ACGT 2 cut(s) 312, 1019
TaqI TCGA 5 cut(s) 9, 57, 328, 686, 993
TasI AATT 5 cut(s) 374, 395, 493, 521, 1223
TatI WGTACW 2 cut(s) 693, 1253
TfiI GAWTC 3 cut(s) 631, 666, 1129
Tru1I TTAA 4 cut(s) 144, 248, 285, 1215
Tru9I TTAA 4 cut(s) 144, 248, 285, 1215
TscAI CASTG 2 cut(s) 549, 808
TseFI GTSAC 5 cut(s) 602, 705, 760, 973, 1055
TseI GCWGC 2 cut(s) 206, 932
Tsp45I GTSAC 5 cut(s) 602, 705, 760, 973, 1055
TspDTI ATGAA 8 cut(s) 387, 422, 548, 572, 846, 980, 1151, 1216
TspRI CASTG 2 cut(s) 549, 808
Tth111I GACNNNGTC 2 cut(s) 789, 1087
Vha464I CTTAAG 1 cut(s) 284
VpaK11BI GGWCC 2 cut(s) 785, 1083
XapI RAATTY 4 cut(s) 374, 395, 521, 1223
XhoI CTCGAG 1 cut(s) 685
XmnI GAANNNNTTC 1 cut(s) 1158
XspI CTAG 3 cut(s) 513, 648, 771
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.