pycom05g13670

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0014404
Physical Location & Seq
Reverse (-)
17161607 .. 17163440
1834 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g13670.3

Sequence Viewer

Length: 1263 bp
ATGGGCAATTCGAGCCTCGAGTTCTTGGACGTCGGACTGCTCTTTTTGCTGTCCATGTTTTGCATGGCAAGTGGTTTCGGTAGAGGAGTCAAAGCAGACGGAAGAAGCCTCTTGCAGCATCACAAGGCTAAGAGCTCTAATCTTTTCAACGTCAAGAGCTTCGGCGCCCGAGCAGACGGCCGAACTGATGACAGCGATGCATTCACAGCGGCATGGAAGGAGGCATGCCAGTCCACAGGTAGAGTCCACCTTGTAATACCGAAAGGAACTTACACGATCGGCCCTCTGAGATTCTCCGGCCCCTGCGTAAATGTTACGTCTCTGAATTTTCGTGTGAAGGGTTACTTGAAGGCTACAACTGACTTGCGCAAGTATGGATCGGGTGGTGGTTGGATTGAATTCGCGTGGATGAAGGGGCTAACCTTGACCGGAGGTGGAACCTTTGATGGCCGAGGGGCTCATGCCTGGCCTCACAACAAGTGCGTCACCAACTCCAACTGCAAACTTCTTCCAACTAACTTGAAATTTGTGGCACTTAACAAGACAGTTATCCGCGGCTTAACTTCCGTGAACAGCAAGTTCTTTCACATAGCTCTTGTAGAATGTCACAACTTCAAAGGTAGCAGGCTCAAGATTTCAGCACCGGAAACTAGCCCTAACACAGATGGAATCCACATCGAGCGAAGCTCTAGCGTCTACTTCTCTTCGTCGCGCATTGCTACTGGTGATGACTGCATCTCAGTTGGACAAGGAAACTCTCAGGTCACCATAACAAGCATCACTTGTGGACCTGGTCATGGCATCAGTGTGGGAAGTCTAGGAAAATATCCGGACGAAAGAGATGTCAGCGGACTCGTAGTGAAGGACTGCACCATGACAGGCACCACCAATGGAATTAGGATCAAGACATGGGCTAACTCTCCAGGAAGCAGTGCTGCCACGAATATGACATTCGAAAACATTGTCATGAACAACGTGACTAATCCGATCATAATCGATCAGTCCTATTGTCCATTCACCGCTTGCACTTCCAAGGCACCATCTAGAGTGAAGCTGAGTGACATATACTTCAAGAACATAAGAGGAACTTCGTCATCGGAGATGGCGGTGTTACTTGAATGCAGCAAAGGGATTCCATGCCAGAATATTTTCCTTGAAGATGTTCACTTGGATTTGTCATTCGGAGAGAAACTAGCCACTTCAAGTTGCAGTAATGTTAAAGCTAAATTTATTGGCACCCAAATCCCACCACCTTGTACTTAA

Protein Analysis

421

Amino Acids

45.06

Weight (kDa)

9.15

Isoelectric Point (pI)

35.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014404)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G18180
fragaria_vesca FvH4_2g16950
malus_domestica MD05G1141300.v1.1 MD05G1142200.v1.1
prunus_persica Prupe.8G181300_v2.0.a1
pyrus_communis pycom05g13670
rosa_chinensis RchiOBHm_Chr6g0281151
rosa_laevigata RLG00000012990
rosa_multiflora Rmu_co8202118.1_g000001 Rmu_sc0035236.1_g000002
rosa_roxburghii Rroxscaffold_7G00187250
rosa_rugosa Rorug06G0139300
rosa_samantha Rh6AG251100 Rh6BG254300 Rh6CG253700 Rh6DG245700
rosa_wichuraiana Rw6G021800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 33
Acc16I TGCGCA 1 cut(s) 368
AccB1I GGYRCC 4 cut(s) 164, 881, 1036, 1235
AccI GTMKAC 1 cut(s) 696
AccII CGCG 3 cut(s) 404, 555, 712
AccIII TCCGGA 1 cut(s) 829
AciI CCGC 6 cut(s) 209, 553, 555, 849, 1020, 1106
AclWI GGATC 2 cut(s) 385, 908
AcoI YGGCCR 2 cut(s) 178, 448
AcsI RAATTY 4 cut(s) 325, 398, 524, 1226
AcyI GRCGYC 2 cut(s) 30, 165
AfaI GTAC 1 cut(s) 1258
AfiI CCNNNNNNNGG 1 cut(s) 797
AgsI TTSAA 9 cut(s) 148, 349, 398, 523, 616, 1072, 1118, 1157, 1203
AjnI CCWGG 3 cut(s) 464, 790, 922
AluBI AGCT 6 cut(s) 135, 159, 593, 687, 1054, 1223
AluI AGCT 6 cut(s) 135, 159, 593, 687, 1054, 1223
Alw21I GWGCWC 1 cut(s) 137
Alw26I GTCTC 1 cut(s) 324
AlwI GGATC 2 cut(s) 385, 908
Ama87I CYCGRG 2 cut(s) 17, 168
Aor13HI TCCGGA 1 cut(s) 829
AoxI GGCC 5 cut(s) 178, 280, 298, 448, 467
ApeKI GCWGC 3 cut(s) 115, 935, 1122
ApoI RAATTY 4 cut(s) 325, 398, 524, 1226
ArsI GACNNNNNNTTYG 4 cut(s) 828, 860, 948, 980
Asp700I GAANNNNTTC 2 cut(s) 1148, 1161
AspLEI GCGC 3 cut(s) 167, 369, 714
AspS9I GGNCC 3 cut(s) 281, 299, 788
AsuHPI GGTGA 4 cut(s) 478, 737, 757, 1009
AsuII TTCGAA 1 cut(s) 954
AvaI CYCGRG 2 cut(s) 17, 168
AvaII GGWCC 1 cut(s) 788
BanI GGYRCC 4 cut(s) 164, 881, 1036, 1235
BanII GRGCYC 2 cut(s) 137, 460
Bbv12I GWGCWC 1 cut(s) 137
BbvI GCAGC 3 cut(s) 127, 922, 1134
BccI CCATC 4 cut(s) 440, 659, 1048, 1096
BceAI ACGGC 1 cut(s) 193
BciT130I CCWGG 3 cut(s) 466, 792, 924
BcoDI GTCTC 1 cut(s) 324
BfaI CTAG 5 cut(s) 651, 690, 818, 1044, 1193
BfoI RGCGCY 1 cut(s) 168
BisI GCNGC 5 cut(s) 116, 210, 556, 936, 1123
BlsI GCNGC 5 cut(s) 117, 211, 557, 937, 1124
Bme1390I CCNGG 3 cut(s) 466, 792, 924
Bme18I GGWCC 1 cut(s) 788
BmeT110I CYCGRG 2 cut(s) 17, 168
BmgT120I GGNCC 3 cut(s) 281, 299, 788
BmiI GGNNCC 6 cut(s) 166, 301, 439, 883, 1038, 1237
BmrFI CCNGG 3 cut(s) 466, 792, 924
BmsI GCATC 5 cut(s) 127, 187, 744, 786, 810
BplI GAGNNNNNCTC 2 cut(s) 671, 703
BpmI CTGGAG 1 cut(s) 906
Bpu14I TTCGAA 1 cut(s) 954
BpuEI CTTGAG 1 cut(s) 614
Bsa29I ATCGAT 1 cut(s) 996
BsaBI GATNNNNATC 1 cut(s) 992
BsaHI GRCGYC 2 cut(s) 30, 165
BsaJI CCNNGG 3 cut(s) 451, 553, 1032
BsaWI WCCGGW 3 cut(s) 428, 643, 829
Bsc4I CCNNNNNNNGG 1 cut(s) 797
Bse1I ACTGG 2 cut(s) 229, 727
Bse3DI GCAATG 1 cut(s) 714
Bse8I GATNNNNATC 1 cut(s) 992
BseAI TCCGGA 1 cut(s) 829
BseBI CCWGG 3 cut(s) 466, 792, 924
BseCI ATCGAT 1 cut(s) 996
BseDI CCNNGG 3 cut(s) 451, 553, 1032
BseGI GGATG 1 cut(s) 414
BseJI GATNNNNATC 1 cut(s) 992
BseLI CCNNNNNNNGG 1 cut(s) 797
BseMI GCAATG 1 cut(s) 714
BseMII CTCAG 4 cut(s) 278, 753, 773, 1046
BseNI ACTGG 2 cut(s) 229, 727
BseRI GAGGAG 1 cut(s) 99
BseX3I CGGCCG 1 cut(s) 178
BseXI GCAGC 3 cut(s) 127, 922, 1134
BsgI GTGCAG 1 cut(s) 853
Bsh1236I CGCG 3 cut(s) 404, 555, 712
Bsh1285I CGRYCG 2 cut(s) 181, 279
BshFI GGCC 5 cut(s) 180, 282, 300, 450, 469
BshNI GGYRCC 4 cut(s) 164, 881, 1036, 1235
BshVI ATCGAT 1 cut(s) 996
BsiEI CGRYCG 2 cut(s) 181, 279
BsiHKAI GWGCWC 1 cut(s) 137
BsiHKCI CYCGRG 2 cut(s) 17, 168
BsiSI CCGG 4 cut(s) 297, 429, 644, 830
BslI CCNNNNNNNGG 1 cut(s) 797
BsmAI GTCTC 1 cut(s) 324
BsmBI CGTCTC 1 cut(s) 324
BsmI GAATGC 2 cut(s) 200, 1124
BsnI GGCC 5 cut(s) 180, 282, 300, 450, 469
BsoBI CYCGRG 2 cut(s) 17, 168
Bsp119I TTCGAA 1 cut(s) 954
Bsp1286I GDGCHC 2 cut(s) 137, 460
Bsp13I TCCGGA 1 cut(s) 829
Bsp143I GATC 5 cut(s) 276, 377, 900, 987, 997
BspACI CCGC 6 cut(s) 209, 553, 555, 849, 1020, 1106
BspANI GGCC 5 cut(s) 180, 282, 300, 450, 469
BspCNI CTCAG 4 cut(s) 279, 752, 772, 1047
BspDI ATCGAT 1 cut(s) 996
BspEI TCCGGA 1 cut(s) 829
BspFNI CGCG 3 cut(s) 404, 555, 712
BspHI TCATGA 1 cut(s) 966
BspLI GGNNCC 6 cut(s) 166, 301, 439, 883, 1038, 1237
BspPI GGATC 2 cut(s) 385, 908
BspT104I TTCGAA 1 cut(s) 954
BspT107I GGYRCC 4 cut(s) 164, 881, 1036, 1235
BsrDI GCAATG 1 cut(s) 714
BsrI ACTGG 2 cut(s) 229, 727
BssECI CCNNGG 3 cut(s) 451, 553, 1032
BssMI GATC 5 cut(s) 276, 377, 900, 987, 997
BssNI GRCGYC 2 cut(s) 30, 165
BssT1I CCWWGG 1 cut(s) 1032
Bst2UI CCWGG 3 cut(s) 466, 792, 924
Bst4CI ACNGT 1 cut(s) 547
Bst6I CTCTTC 1 cut(s) 709
BstACI GRCGYC 2 cut(s) 30, 165
BstBI TTCGAA 1 cut(s) 954
BstC8I GCNNGC 3 cut(s) 226, 626, 1024
BstDEI CTNAG 5 cut(s) 129, 287, 739, 759, 1055
BstDSI CCRYGG 1 cut(s) 553
BstEII GGTNACC 1 cut(s) 763
BstF5I GGATG 1 cut(s) 414
BstFNI CGCG 3 cut(s) 404, 555, 712
BstH2I RGCGCY 1 cut(s) 168
BstHHI GCGC 3 cut(s) 167, 369, 714
BstKTI GATC 5 cut(s) 279, 380, 903, 990, 1000
BstMAI GTCTC 1 cut(s) 324
BstMBI GATC 5 cut(s) 276, 377, 900, 987, 997
BstMCI CGRYCG 2 cut(s) 181, 279
BstMWI GCNNNNNNNGC 3 cut(s) 12, 46, 206
BstNI CCWGG 3 cut(s) 466, 792, 924
BstNSI RCATGY 1 cut(s) 228
BstPI GGTNACC 1 cut(s) 763
BstSCI CCNGG 3 cut(s) 464, 790, 922
BstUI CGCG 3 cut(s) 404, 555, 712
BstV1I GCAGC 3 cut(s) 127, 922, 1134
BstZI CGGCCG 1 cut(s) 178
Bsu15I ATCGAT 1 cut(s) 996
BsuRI GGCC 5 cut(s) 180, 282, 300, 450, 469
BsuTUI ATCGAT 1 cut(s) 996
BtgI CCRYGG 1 cut(s) 553
BtgZI GCGATG 1 cut(s) 210
BtsCI GGATG 1 cut(s) 414
BtsI GCAGTG 1 cut(s) 937
BtsIMutI CAGTG 2 cut(s) 811, 937
Cac8I GCNNGC 3 cut(s) 226, 626, 1024
CciI TCATGA 1 cut(s) 966
CfoI GCGC 3 cut(s) 167, 369, 714
Cfr13I GGNCC 3 cut(s) 281, 299, 788
Cfr42I CCGCGG 1 cut(s) 556
ClaI ATCGAT 1 cut(s) 996
CseI GACGC 2 cut(s) 472, 682
CsiI ACCWGGT 1 cut(s) 790
Csp6I GTAC 1 cut(s) 1257
CviQI GTAC 1 cut(s) 1257
DdeI CTNAG 5 cut(s) 129, 287, 739, 759, 1055
DinI GGCGCC 1 cut(s) 166
DpnI GATC 5 cut(s) 278, 379, 902, 989, 999
DpnII GATC 5 cut(s) 276, 377, 900, 987, 997
EaeI YGGCCR 2 cut(s) 178, 448
EagI CGGCCG 1 cut(s) 178
Eam1104I CTCTTC 1 cut(s) 709
EarI CTCTTC 1 cut(s) 709
Ecl136II GAGCTC 1 cut(s) 135
EclXI CGGCCG 1 cut(s) 178
Eco130I CCWWGG 1 cut(s) 1032
Eco24I GRGCYC 2 cut(s) 137, 460
Eco47I GGWCC 1 cut(s) 788
Eco52I CGGCCG 1 cut(s) 178
Eco53kI GAGCTC 1 cut(s) 135
Eco88I CYCGRG 2 cut(s) 17, 168
Eco91I GGTNACC 1 cut(s) 763
EcoICRI GAGCTC 1 cut(s) 135
EcoO65I GGTNACC 1 cut(s) 763
EcoRI GAATTC 1 cut(s) 398
EcoRII CCWGG 3 cut(s) 464, 790, 922
EcoT14I CCWWGG 1 cut(s) 1032
EcoT22I ATGCAT 1 cut(s) 202
EcoT38I GRGCYC 2 cut(s) 137, 460
EgeI GGCGCC 1 cut(s) 166
EheI GGCGCC 1 cut(s) 166
ErhI CCWWGG 1 cut(s) 1032
Esp3I CGTCTC 1 cut(s) 324
FalI AAGNNNNNCTT 6 cut(s) 329, 361, 766, 798, 1072, 1104
FblI GTMKAC 1 cut(s) 696
Fnu4HI GCNGC 5 cut(s) 116, 210, 556, 936, 1123
FokI GGATG 1 cut(s) 421
FriOI GRGCYC 2 cut(s) 137, 460
Fsp4HI GCNGC 5 cut(s) 116, 210, 556, 936, 1123
FspBI CTAG 5 cut(s) 651, 690, 818, 1044, 1193
FspI TGCGCA 1 cut(s) 368
GlaI GCGC 3 cut(s) 166, 368, 713
GluI GCNGC 5 cut(s) 116, 210, 556, 936, 1123
GsuI CTGGAG 1 cut(s) 906
HaeII RGCGCY 1 cut(s) 168
HaeIII GGCC 5 cut(s) 180, 282, 300, 450, 469
HapII CCGG 4 cut(s) 297, 429, 644, 830
HgaI GACGC 2 cut(s) 472, 682
HhaI GCGC 3 cut(s) 167, 369, 714
Hin1I GRCGYC 2 cut(s) 30, 165
Hin6I GCGC 3 cut(s) 165, 367, 712
HinP1I GCGC 3 cut(s) 165, 367, 712
HinfI GANTC 6 cut(s) 87, 243, 291, 669, 852, 1132
HpaII CCGG 4 cut(s) 297, 429, 644, 830
HphI GGTGA 4 cut(s) 478, 737, 757, 1009
Hpy166II GTNNAC 6 cut(s) 234, 247, 571, 697, 788, 1165
Hpy188I TCNGA 6 cut(s) 35, 288, 324, 987, 1099, 1184
Hpy188III TCNNGA 7 cut(s) 154, 631, 830, 904, 967, 1044, 1072
Hpy8I GTNNAC 6 cut(s) 234, 247, 571, 697, 788, 1165
Hpy99I CGWCG 2 cut(s) 35, 712
HpyAV CCTTC 5 cut(s) 211, 331, 343, 406, 856
HpyCH4III ACNGT 1 cut(s) 547
HpyCH4IV ACGT 4 cut(s) 30, 150, 317, 975
HpyCH4V TGCA 9 cut(s) 63, 115, 200, 501, 735, 870, 1026, 1122, 1209
HpyF10VI GCNNNNNNNGC 3 cut(s) 12, 46, 206
HpyF3I CTNAG 5 cut(s) 129, 287, 739, 759, 1055
HpySE526I ACGT 4 cut(s) 30, 150, 317, 975
Hsp92I GRCGYC 2 cut(s) 30, 165
HspAI GCGC 3 cut(s) 165, 367, 712
KasI GGCGCC 1 cut(s) 164
Kpn2I TCCGGA 1 cut(s) 829
KspI CCGCGG 1 cut(s) 556
Kzo9I GATC 5 cut(s) 276, 377, 900, 987, 997
Lsp1109I GCAGC 3 cut(s) 127, 922, 1134
LweI GCATC 5 cut(s) 127, 187, 744, 786, 810
MabI ACCWGGT 1 cut(s) 790
MaeI CTAG 5 cut(s) 651, 690, 818, 1044, 1193
MaeII ACGT 4 cut(s) 30, 150, 317, 975
MaeIII GTNAC 8 cut(s) 313, 341, 484, 605, 763, 976, 1058, 1110
MalI GATC 5 cut(s) 278, 379, 902, 989, 999
MboI GATC 5 cut(s) 276, 377, 900, 987, 997
MboII GAAGA 4 cut(s) 114, 500, 696, 1169
MhlI GDGCHC 2 cut(s) 137, 460
MluCI AATT 6 cut(s) 7, 325, 398, 524, 894, 1226
Mly113I GGCGCC 1 cut(s) 165
MlyI GAGTC 3 cut(s) 96, 252, 846
MmeI TCCRAC 5 cut(s) 13, 371, 519, 536, 724
MnlI CCTC 9 cut(s) 26, 77, 119, 214, 294, 425, 446, 480, 1076
Mph1103I ATGCAT 1 cut(s) 202
MroI TCCGGA 1 cut(s) 829
MroXI GAANNNNTTC 2 cut(s) 1148, 1161
MseI TTAA 4 cut(s) 537, 560, 1218, 1261
MslI CAYNNNNRTG 3 cut(s) 806, 944, 965
MspA1I CMGCKG 3 cut(s) 209, 555, 849
MspI CCGG 4 cut(s) 297, 429, 644, 830
MspR9I CCNGG 3 cut(s) 466, 792, 924
Mva1269I GAATGC 2 cut(s) 200, 1124
MvaI CCWGG 3 cut(s) 466, 792, 924
MvnI CGCG 3 cut(s) 404, 555, 712
MwoI GCNNNNNNNGC 3 cut(s) 12, 46, 206
NarI GGCGCC 1 cut(s) 165
NdeII GATC 5 cut(s) 276, 377, 900, 987, 997
NlaIV GGNNCC 6 cut(s) 166, 301, 439, 883, 1038, 1237
NmeAIII GCCGAG 1 cut(s) 476
NmuCI GTSAC 5 cut(s) 484, 605, 763, 976, 1058
NsbI TGCGCA 1 cut(s) 368
NsiI ATGCAT 1 cut(s) 202
NspI RCATGY 1 cut(s) 228
NspV TTCGAA 1 cut(s) 954
PaeI GCATGC 1 cut(s) 228
PaeR7I CTCGAG 1 cut(s) 17
PagI TCATGA 1 cut(s) 966
PctI GAATGC 2 cut(s) 200, 1124
PdmI GAANNNNTTC 2 cut(s) 1148, 1161
PfeI GAWTC 3 cut(s) 291, 669, 1132
PflFI GACNNNGTC 1 cut(s) 792
PfoI TCCNGGA 1 cut(s) 922
PkrI GCNGC 5 cut(s) 117, 211, 557, 937, 1124
Ple19I CGATCG 1 cut(s) 279
PleI GAGTC 3 cut(s) 95, 251, 846
PluTI GGCGCC 1 cut(s) 168
PpsI GAGTC 3 cut(s) 95, 251, 846
Psp124BI GAGCTC 1 cut(s) 137
Psp6I CCWGG 3 cut(s) 464, 790, 922
PspEI GGTNACC 1 cut(s) 763
PspGI CCWGG 3 cut(s) 464, 790, 922
PspN4I GGNNCC 6 cut(s) 166, 301, 439, 883, 1038, 1237
PspPI GGNCC 3 cut(s) 281, 299, 788
PspXI VCTCGAGB 1 cut(s) 17
PsyI GACNNNGTC 1 cut(s) 792
PvuI CGATCG 1 cut(s) 279
RsaI GTAC 1 cut(s) 1258
RsaNI GTAC 1 cut(s) 1257
RseI CAYNNNNRTG 3 cut(s) 806, 944, 965
SacI GAGCTC 1 cut(s) 137
SacII CCGCGG 1 cut(s) 556
SaqAI TTAA 4 cut(s) 537, 560, 1218, 1261
SatI GCNGC 5 cut(s) 116, 210, 556, 936, 1123
Sau3AI GATC 5 cut(s) 276, 377, 900, 987, 997
Sau96I GGNCC 3 cut(s) 281, 299, 788
SchI GAGTC 3 cut(s) 96, 252, 846
ScrFI CCNGG 3 cut(s) 466, 792, 924
SduI GDGCHC 2 cut(s) 137, 460
SexAI ACCWGGT 1 cut(s) 790
SfaNI GCATC 5 cut(s) 127, 187, 744, 786, 810
SfoI GGCGCC 1 cut(s) 166
Sfr274I CTCGAG 1 cut(s) 17
Sfr303I CCGCGG 1 cut(s) 556
SfuI TTCGAA 1 cut(s) 954
SgrBI CCGCGG 1 cut(s) 556
SinI GGWCC 1 cut(s) 788
SlaI CTCGAG 1 cut(s) 17
SmiMI CAYNNNNRTG 3 cut(s) 806, 944, 965
SmlI CTYRAG 2 cut(s) 17, 629
SmoI CTYRAG 2 cut(s) 17, 629
SphI GCATGC 1 cut(s) 228
Sse9I AATT 6 cut(s) 7, 325, 398, 524, 894, 1226
SsiI CCGC 6 cut(s) 209, 553, 555, 849, 1020, 1106
SspDI GGCGCC 1 cut(s) 164
SspI AATATT 1 cut(s) 1147
SspMI CTAG 5 cut(s) 651, 690, 818, 1044, 1193
SstI GAGCTC 1 cut(s) 137
StyD4I CCNGG 3 cut(s) 464, 790, 922
StyI CCWWGG 1 cut(s) 1032
TaaI ACNGT 1 cut(s) 547
TaiI ACGT 4 cut(s) 33, 153, 320, 978
TaqI TCGA 5 cut(s) 11, 18, 678, 954, 996
TasI AATT 6 cut(s) 7, 325, 398, 524, 894, 1226
TatI WGTACW 1 cut(s) 1256
TauI GCSGC 2 cut(s) 212, 558
TfiI GAWTC 3 cut(s) 291, 669, 1132
Tru1I TTAA 4 cut(s) 537, 560, 1218, 1261
Tru9I TTAA 4 cut(s) 537, 560, 1218, 1261
TscAI CASTG 2 cut(s) 811, 937
TseFI GTSAC 5 cut(s) 484, 605, 763, 976, 1058
TseI GCWGC 3 cut(s) 115, 935, 1122
Tsp45I GTSAC 5 cut(s) 484, 605, 763, 976, 1058
TspDTI ATGAA 2 cut(s) 425, 983
TspGWI ACGGA 2 cut(s) 114, 556
TspRI CASTG 2 cut(s) 811, 937
Tth111I GACNNNGTC 1 cut(s) 792
VpaK11BI GGWCC 1 cut(s) 788
XapI RAATTY 4 cut(s) 325, 398, 524, 1226
XbaI TCTAGA 1 cut(s) 1043
XceI RCATGY 1 cut(s) 228
XcmI CCANNNNNNNNNTGG 1 cut(s) 61
XhoI CTCGAG 1 cut(s) 17
XmiI GTMKAC 1 cut(s) 696
XmnI GAANNNNTTC 2 cut(s) 1148, 1161
XspI CTAG 5 cut(s) 651, 690, 818, 1044, 1193
ZraI GACGTC 1 cut(s) 31
Zsp2I ATGCAT 1 cut(s) 202
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.