Prupe.8G181300_v2.0.a1

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0014404
Physical Location & Seq
Reverse (-)
18154229 .. 18155951
1723 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G181300.1

Sequence Viewer

Length: 1164 bp
ATGAAAGCAGAAGGAAGAAGCCTGCATCACAGGGCTAAGAACTCTGCTGTTTTTGACGTGATGAGCTTCGGCGCCCGAGCGGACGGTCGAACTGATGACAGCAATGCCTTCATGGCAGCATGGAAGGAGGCATGCCAGTCTACAGGTAGGGCACACATTACGATACCGAAAGGGACATACATAATTGGCCCTCTTAAATTCTCAGGCCCCTGCCTAAACGTTTCGTCTCTGACGATTCGCGGTTATTTGAAGGCTACAACGGACCTGCGCAAGTATGGATTTGGTGGTAGTTGGCTCGAATTTGGGTGGATGGAGGGGCTAACCTTGACCGGAGGTGGAACCTTTGATGGCCGAGGTGCTCGAGCCTGGCCTCACAACAAGTGCCCCACAGACTCCAACTGCAAGCTTCTTCCAACTAGTTTGAAATTTGTGGCAATGAATAAGACTGTCATCCGAGGCTTAACTTCAGTGAACAGCAAGTTCTTTCACATAGCTCTTGTAGAATGTCACAACTTCAAAGGTAGCAGGCTCAAGATTTCAGCCCCCGAAACAAGTCCTAACACCGATGGCATCCACATCGAACGAAGCTCTAGTGTCTACTTCTCTCTGTCGCATATTGCTACCGGTGATGACTGCATCTCGGTTGGACAAGGAAATTCTCAGATCACCATAACAAGCATCACTTGTGGACCTGGCCATGGCATCAGTGTGGGAAGTCTAGGAAAATATCCAGACGAAAGAGATGTTAGTGGACTTGTAGTGAAAGACTGCACCATGACAGGTACCACCAACGGCATTAGGATCAAGACATGGGCAAACTCTCCCGGCAACAGTGCAGCCACGAACATGACATTCGAAAACATTGTCATGAATAACGTGACCAATCCGATCATAATCGATCAATTGTATTGTCCATTCACATCCTGCACTTCTAAGGCACCATCCAGAGTGAAGCTGAGTGACATATACTTCAAGAACATAAGAGGGACATCATCGTCGGAGGTGGCAGTGGCTCTTGAATGCAGCAAAGGGATCCCATGCCAGAATATTTACCTTGAAGATGTTCACTTGGACCTGTCATCTGGGGAGAAACAAGCTACTTCAAGTTGCAGCAATGTCAAAGCTAAATTCAGTGGCACCCAAATCCCACCTCCATGTACTTAG

Protein Analysis

388

Amino Acids

41.55

Weight (kDa)

8.93

Isoelectric Point (pI)

39.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014404)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G18180
fragaria_vesca FvH4_2g16950
malus_domestica MD05G1141300.v1.1 MD05G1142200.v1.1
prunus_persica Prupe.8G181300_v2.0.a1
pyrus_communis pycom05g13670
rosa_chinensis RchiOBHm_Chr6g0281151
rosa_laevigata RLG00000012990
rosa_multiflora Rmu_co8202118.1_g000001 Rmu_sc0035236.1_g000002
rosa_roxburghii Rroxscaffold_7G00187250
rosa_rugosa Rorug06G0139300
rosa_samantha Rh6AG251100 Rh6BG254300 Rh6CG253700 Rh6DG245700
rosa_wichuraiana Rw6G021800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 994
Acc16I TGCGCA 1 cut(s) 269
Acc36I ACCTGC 1 cut(s) 273
Acc65I GGTACC 1 cut(s) 782
AccB1I GGYRCC 4 cut(s) 71, 782, 937, 1136
AccBSI CCGCTC 1 cut(s) 80
AccI GTMKAC 2 cut(s) 140, 597
AccII CGCG 1 cut(s) 240
AciI CCGC 2 cut(s) 80, 240
AclI AACGTT 1 cut(s) 219
AclWI GGATC 3 cut(s) 809, 1027, 1040
AcoI YGGCCR 2 cut(s) 349, 694
AcsI RAATTY 5 cut(s) 197, 299, 425, 655, 1127
AcuI CTGAAG 1 cut(s) 450
AcyI GRCGYC 1 cut(s) 72
AfaI GTAC 2 cut(s) 784, 1159
AfiI CCNNNNNNNGG 1 cut(s) 698
AgeI ACCGGT 1 cut(s) 623
AgsI TTSAA 7 cut(s) 250, 424, 517, 973, 1019, 1058, 1104
AhlI ACTAGT 1 cut(s) 416
AjiI CACGTC 1 cut(s) 58
AjnI CCWGG 2 cut(s) 365, 691
AluBI AGCT 7 cut(s) 66, 406, 494, 588, 955, 1097, 1124
AluI AGCT 7 cut(s) 66, 406, 494, 588, 955, 1097, 1124
Alw21I GWGCWC 1 cut(s) 361
Alw26I GTCTC 1 cut(s) 231
AlwI GGATC 3 cut(s) 809, 1027, 1040
Ama87I CYCGRG 2 cut(s) 75, 360
AoxI GGCC 5 cut(s) 187, 205, 349, 368, 694
ApeKI GCWGC 4 cut(s) 116, 836, 1023, 1110
ApoI RAATTY 5 cut(s) 197, 299, 425, 655, 1127
ArsI GACNNNNNNTTYG 2 cut(s) 849, 881
AsiGI ACCGGT 1 cut(s) 623
Asp700I GAANNNNTTC 1 cut(s) 1062
Asp718I GGTACC 1 cut(s) 782
AspLEI GCGC 2 cut(s) 74, 270
AspS9I GGNCC 5 cut(s) 188, 206, 262, 689, 1072
AsuC2I CCSGG 1 cut(s) 825
AsuHPI GGTGA 2 cut(s) 638, 658
AsuII TTCGAA 1 cut(s) 855
AvaI CYCGRG 2 cut(s) 75, 360
AvaII GGWCC 3 cut(s) 262, 689, 1072
BaeGI GKGCMC 2 cut(s) 154, 386
BalI TGGCCA 1 cut(s) 696
BamHI GGATCC 1 cut(s) 1032
BanI GGYRCC 4 cut(s) 71, 782, 937, 1136
Bbv12I GWGCWC 1 cut(s) 361
BbvI GCAGC 4 cut(s) 128, 848, 1035, 1122
BccI CCATC 4 cut(s) 304, 341, 560, 949
BceAI ACGGC 1 cut(s) 808
BcgI CGANNNNNNTGC 2 cut(s) 559, 593
BciT130I CCWGG 2 cut(s) 367, 693
BcnI CCSGG 1 cut(s) 825
BcoDI GTCTC 1 cut(s) 231
BcuI ACTAGT 1 cut(s) 416
BfaI CTAG 3 cut(s) 417, 591, 719
BfmI CTRYAG 1 cut(s) 141
BfoI RGCGCY 1 cut(s) 75
BfuAI ACCTGC 1 cut(s) 273
BglI GCCNNNNNGGC 1 cut(s) 113
BisI GCNGC 4 cut(s) 117, 837, 1024, 1111
BlsI GCNGC 4 cut(s) 118, 838, 1025, 1112
Bme1390I CCNGG 3 cut(s) 367, 693, 825
Bme18I GGWCC 3 cut(s) 262, 689, 1072
BmeT110I CYCGRG 2 cut(s) 75, 360
BmgBI CACGTC 1 cut(s) 58
BmgT120I GGNCC 5 cut(s) 188, 206, 262, 689, 1072
BmiI GGNNCC 7 cut(s) 73, 208, 340, 784, 939, 1034, 1138
BmrFI CCNGG 3 cut(s) 367, 693, 825
BmsI GCATC 5 cut(s) 34, 579, 645, 687, 711
Bpu14I TTCGAA 1 cut(s) 855
BpuEI CTTGAG 1 cut(s) 515
BpuMI CCSGG 1 cut(s) 825
Bsa29I ATCGAT 1 cut(s) 897
BsaBI GATNNNNATC 1 cut(s) 893
BsaHI GRCGYC 1 cut(s) 72
BsaJI CCNNGG 3 cut(s) 352, 454, 697
BsaWI WCCGGW 2 cut(s) 329, 623
Bsc4I CCNNNNNNNGG 1 cut(s) 698
Bse118I RCCGGY 1 cut(s) 623
Bse1I ACTGG 1 cut(s) 136
Bse3DI GCAATG 3 cut(s) 109, 441, 1120
Bse8I GATNNNNATC 1 cut(s) 893
BseBI CCWGG 2 cut(s) 367, 693
BseCI ATCGAT 1 cut(s) 897
BseDI CCNNGG 3 cut(s) 352, 454, 697
BseGI GGATG 5 cut(s) 315, 450, 570, 920, 941
BseJI GATNNNNATC 1 cut(s) 893
BseLI CCNNNNNNNGG 1 cut(s) 698
BseMI GCAATG 3 cut(s) 109, 441, 1120
BseMII CTCAG 3 cut(s) 216, 674, 947
BseNI ACTGG 1 cut(s) 136
BseSI GKGCMC 2 cut(s) 154, 386
BseXI GCAGC 4 cut(s) 128, 848, 1035, 1122
BsgI GTGCAG 3 cut(s) 754, 855, 910
Bsh1236I CGCG 1 cut(s) 240
Bsh1285I CGRYCG 1 cut(s) 88
BshFI GGCC 5 cut(s) 189, 207, 351, 370, 696
BshNI GGYRCC 4 cut(s) 71, 782, 937, 1136
BshTI ACCGGT 1 cut(s) 623
BshVI ATCGAT 1 cut(s) 897
BsiEI CGRYCG 1 cut(s) 88
BsiHKAI GWGCWC 1 cut(s) 361
BsiHKCI CYCGRG 2 cut(s) 75, 360
BsiSI CCGG 3 cut(s) 330, 624, 825
BslFI GGGAC 2 cut(s) 187, 1000
BslI CCNNNNNNNGG 1 cut(s) 698
BsmAI GTCTC 1 cut(s) 231
BsmBI CGTCTC 1 cut(s) 231
BsmFI GGGAC 2 cut(s) 187, 1000
BsmI GAATGC 1 cut(s) 1025
BsnI GGCC 5 cut(s) 189, 207, 351, 370, 696
BsoBI CYCGRG 2 cut(s) 75, 360
Bsp119I TTCGAA 1 cut(s) 855
Bsp1286I GDGCHC 3 cut(s) 154, 361, 386
Bsp143I GATC 5 cut(s) 663, 801, 888, 898, 1032
Bsp19I CCATGG 1 cut(s) 697
BspACI CCGC 2 cut(s) 80, 240
BspANI GGCC 5 cut(s) 189, 207, 351, 370, 696
BspCNI CTCAG 3 cut(s) 215, 673, 948
BspDI ATCGAT 1 cut(s) 897
BspFNI CGCG 1 cut(s) 240
BspHI TCATGA 1 cut(s) 867
BspLI GGNNCC 7 cut(s) 73, 208, 340, 784, 939, 1034, 1138
BspMI ACCTGC 1 cut(s) 273
BspPI GGATC 3 cut(s) 809, 1027, 1040
BspT104I TTCGAA 1 cut(s) 855
BspT107I GGYRCC 4 cut(s) 71, 782, 937, 1136
BsrBI CCGCTC 1 cut(s) 80
BsrDI GCAATG 3 cut(s) 109, 441, 1120
BsrFI RCCGGY 1 cut(s) 623
BsrI ACTGG 1 cut(s) 136
BssAI RCCGGY 1 cut(s) 623
BssECI CCNNGG 3 cut(s) 352, 454, 697
BssMI GATC 5 cut(s) 663, 801, 888, 898, 1032
BssNI GRCGYC 1 cut(s) 72
BssT1I CCWWGG 1 cut(s) 697
Bst2UI CCWGG 2 cut(s) 367, 693
Bst4CI ACNGT 3 cut(s) 86, 448, 833
BstACI GRCGYC 1 cut(s) 72
BstBI TTCGAA 1 cut(s) 855
BstC8I GCNNGC 4 cut(s) 23, 133, 404, 527
BstDEI CTNAG 6 cut(s) 36, 202, 660, 933, 956, 1161
BstDSI CCRYGG 1 cut(s) 697
BstF5I GGATG 5 cut(s) 315, 450, 570, 920, 941
BstFNI CGCG 1 cut(s) 240
BstH2I RGCGCY 1 cut(s) 75
BstHHI GCGC 2 cut(s) 74, 270
BstKTI GATC 5 cut(s) 666, 804, 891, 901, 1035
BstMAI GTCTC 1 cut(s) 231
BstMBI GATC 5 cut(s) 663, 801, 888, 898, 1032
BstMCI CGRYCG 1 cut(s) 88
BstMWI GCNNNNNNNGC 1 cut(s) 113
BstNI CCWGG 2 cut(s) 367, 693
BstNSI RCATGY 1 cut(s) 135
BstSCI CCNGG 3 cut(s) 365, 691, 823
BstSFI CTRYAG 1 cut(s) 141
BstSLI GKGCMC 2 cut(s) 154, 386
BstUI CGCG 1 cut(s) 240
BstV1I GCAGC 4 cut(s) 128, 848, 1035, 1122
BstX2I RGATCY 1 cut(s) 1032
BstYI RGATCY 1 cut(s) 1032
Bsu15I ATCGAT 1 cut(s) 897
BsuRI GGCC 5 cut(s) 189, 207, 351, 370, 696
BsuTUI ATCGAT 1 cut(s) 897
BtgI CCRYGG 1 cut(s) 697
BtrI CACGTC 1 cut(s) 58
BtsCI GGATG 5 cut(s) 315, 450, 570, 920, 941
BtsI GCAGTG 1 cut(s) 1014
BtsIMutI CAGTG 5 cut(s) 474, 712, 838, 1014, 1138
BveI ACCTGC 1 cut(s) 273
Cac8I GCNNGC 4 cut(s) 23, 133, 404, 527
CciI TCATGA 1 cut(s) 867
CfoI GCGC 2 cut(s) 74, 270
Cfr10I RCCGGY 1 cut(s) 623
Cfr13I GGNCC 5 cut(s) 188, 206, 262, 689, 1072
ClaI ATCGAT 1 cut(s) 897
Csp6I GTAC 2 cut(s) 783, 1158
CspAI ACCGGT 1 cut(s) 623
CviQI GTAC 2 cut(s) 783, 1158
DdeI CTNAG 6 cut(s) 36, 202, 660, 933, 956, 1161
DinI GGCGCC 1 cut(s) 73
DpnI GATC 5 cut(s) 665, 803, 890, 900, 1034
DpnII GATC 5 cut(s) 663, 801, 888, 898, 1032
DrdI GACNNNNNNGTC 1 cut(s) 994
DseDI GACNNNNNNGTC 1 cut(s) 994
EaeI YGGCCR 2 cut(s) 349, 694
Eco130I CCWWGG 1 cut(s) 697
Eco47I GGWCC 3 cut(s) 262, 689, 1072
Eco57I CTGAAG 1 cut(s) 450
Eco88I CYCGRG 2 cut(s) 75, 360
EcoO109I RGGNCCY 1 cut(s) 206
EcoRII CCWGG 2 cut(s) 365, 691
EcoT14I CCWWGG 1 cut(s) 697
EgeI GGCGCC 1 cut(s) 73
EheI GGCGCC 1 cut(s) 73
ErhI CCWWGG 1 cut(s) 697
Esp3I CGTCTC 1 cut(s) 231
FalI AAGNNNNNCTT 2 cut(s) 667, 699
FaqI GGGAC 2 cut(s) 187, 1000
FblI GTMKAC 2 cut(s) 140, 597
Fnu4HI GCNGC 4 cut(s) 117, 837, 1024, 1111
FokI GGATG 5 cut(s) 322, 437, 557, 907, 928
Fsp4HI GCNGC 4 cut(s) 117, 837, 1024, 1111
FspBI CTAG 3 cut(s) 417, 591, 719
FspI TGCGCA 1 cut(s) 269
GlaI GCGC 2 cut(s) 73, 269
GluI GCNGC 4 cut(s) 117, 837, 1024, 1111
HaeII RGCGCY 1 cut(s) 75
HaeIII GGCC 5 cut(s) 189, 207, 351, 370, 696
HapII CCGG 3 cut(s) 330, 624, 825
HhaI GCGC 2 cut(s) 74, 270
Hin1I GRCGYC 1 cut(s) 72
Hin6I GCGC 2 cut(s) 72, 268
HinP1I GCGC 2 cut(s) 72, 268
HindIII AAGCTT 1 cut(s) 404
HinfI GANTC 2 cut(s) 235, 392
HpaII CCGG 3 cut(s) 330, 624, 825
HphI GGTGA 2 cut(s) 638, 658
Hpy166II GTNNAC 6 cut(s) 141, 472, 598, 689, 752, 1066
Hpy188I TCNGA 5 cut(s) 231, 455, 663, 888, 1000
Hpy188III TCNNGA 7 cut(s) 532, 731, 805, 868, 945, 973, 1016
Hpy8I GTNNAC 6 cut(s) 141, 472, 598, 689, 752, 1066
Hpy99I CGWCG 1 cut(s) 1000
HpyAV CCTTC 4 cut(s) 5, 118, 118, 244
HpyCH4III ACNGT 3 cut(s) 86, 448, 833
HpyCH4IV ACGT 3 cut(s) 57, 219, 876
HpyCH4V TGCA 8 cut(s) 25, 402, 636, 771, 836, 927, 1023, 1110
HpyF10VI GCNNNNNNNGC 1 cut(s) 113
HpyF3I CTNAG 6 cut(s) 36, 202, 660, 933, 956, 1161
HpySE526I ACGT 3 cut(s) 57, 219, 876
Hsp92I GRCGYC 1 cut(s) 72
HspAI GCGC 2 cut(s) 72, 268
KasI GGCGCC 1 cut(s) 71
KpnI GGTACC 1 cut(s) 786
Kzo9I GATC 5 cut(s) 663, 801, 888, 898, 1032
Lsp1109I GCAGC 4 cut(s) 128, 848, 1035, 1122
LweI GCATC 5 cut(s) 34, 579, 645, 687, 711
MaeI CTAG 3 cut(s) 417, 591, 719
MaeII ACGT 3 cut(s) 57, 219, 876
MaeIII GTNAC 3 cut(s) 506, 877, 959
MalI GATC 5 cut(s) 665, 803, 890, 900, 1034
MbiI CCGCTC 1 cut(s) 80
MboI GATC 5 cut(s) 663, 801, 888, 898, 1032
MboII GAAGA 3 cut(s) 27, 401, 1070
MfeI CAATTG 1 cut(s) 902
MflI RGATCY 1 cut(s) 1032
MhlI GDGCHC 3 cut(s) 154, 361, 386
MlsI TGGCCA 1 cut(s) 696
MluCI AATT 7 cut(s) 183, 197, 299, 425, 655, 902, 1127
MluNI TGGCCA 1 cut(s) 696
Mly113I GGCGCC 1 cut(s) 72
MlyI GAGTC 1 cut(s) 386
MmeI TCCRAC 4 cut(s) 420, 437, 625, 978
Mox20I TGGCCA 1 cut(s) 696
MroXI GAANNNNTTC 1 cut(s) 1062
MscI TGGCCA 1 cut(s) 696
MseI TTAA 2 cut(s) 195, 461
MslI CAYNNNNRTG 4 cut(s) 707, 845, 866, 1153
Msp20I TGGCCA 1 cut(s) 696
MspI CCGG 3 cut(s) 330, 624, 825
MspR9I CCNGG 3 cut(s) 367, 693, 825
MunI CAATTG 1 cut(s) 902
Mva1269I GAATGC 1 cut(s) 1025
MvaI CCWGG 2 cut(s) 367, 693
MvnI CGCG 1 cut(s) 240
MwoI GCNNNNNNNGC 1 cut(s) 113
NarI GGCGCC 1 cut(s) 72
NciI CCSGG 1 cut(s) 825
NcoI CCATGG 1 cut(s) 697
NdeII GATC 5 cut(s) 663, 801, 888, 898, 1032
NlaIV GGNNCC 7 cut(s) 73, 208, 340, 784, 939, 1034, 1138
NmeAIII GCCGAG 1 cut(s) 377
NmuCI GTSAC 3 cut(s) 506, 877, 959
NsbI TGCGCA 1 cut(s) 269
NspI RCATGY 1 cut(s) 135
NspV TTCGAA 1 cut(s) 855
PaeI GCATGC 1 cut(s) 135
PaeR7I CTCGAG 1 cut(s) 360
PagI TCATGA 1 cut(s) 867
PcsI WCGNNNNNNNCGW 1 cut(s) 230
PctI GAATGC 1 cut(s) 1025
PdmI GAANNNNTTC 1 cut(s) 1062
PfeI GAWTC 1 cut(s) 235
PinAI ACCGGT 1 cut(s) 623
PkrI GCNGC 4 cut(s) 118, 838, 1025, 1112
PleI GAGTC 1 cut(s) 386
PluTI GGCGCC 1 cut(s) 75
PpsI GAGTC 1 cut(s) 386
Psp1406I AACGTT 1 cut(s) 219
Psp6I CCWGG 2 cut(s) 365, 691
PspGI CCWGG 2 cut(s) 365, 691
PspN4I GGNNCC 7 cut(s) 73, 208, 340, 784, 939, 1034, 1138
PspPI GGNCC 5 cut(s) 188, 206, 262, 689, 1072
PspXI VCTCGAGB 1 cut(s) 360
PsuI RGATCY 1 cut(s) 1032
RsaI GTAC 2 cut(s) 784, 1159
RsaNI GTAC 2 cut(s) 783, 1158
RseI CAYNNNNRTG 4 cut(s) 707, 845, 866, 1153
SaqAI TTAA 2 cut(s) 195, 461
SatI GCNGC 4 cut(s) 117, 837, 1024, 1111
Sau3AI GATC 5 cut(s) 663, 801, 888, 898, 1032
Sau96I GGNCC 5 cut(s) 188, 206, 262, 689, 1072
SchI GAGTC 1 cut(s) 386
ScrFI CCNGG 3 cut(s) 367, 693, 825
SduI GDGCHC 3 cut(s) 154, 361, 386
SfaNI GCATC 5 cut(s) 34, 579, 645, 687, 711
SfcI CTRYAG 1 cut(s) 141
SfoI GGCGCC 1 cut(s) 73
Sfr274I CTCGAG 1 cut(s) 360
SfuI TTCGAA 1 cut(s) 855
SinI GGWCC 3 cut(s) 262, 689, 1072
SlaI CTCGAG 1 cut(s) 360
SmiMI CAYNNNNRTG 4 cut(s) 707, 845, 866, 1153
SmlI CTYRAG 2 cut(s) 360, 530
SmoI CTYRAG 2 cut(s) 360, 530
SpeI ACTAGT 1 cut(s) 416
SphI GCATGC 1 cut(s) 135
Sse9I AATT 7 cut(s) 183, 197, 299, 425, 655, 902, 1127
SsiI CCGC 2 cut(s) 80, 240
SspDI GGCGCC 1 cut(s) 71
SspI AATATT 1 cut(s) 1048
SspMI CTAG 3 cut(s) 417, 591, 719
StyD4I CCNGG 3 cut(s) 365, 691, 823
StyI CCWWGG 1 cut(s) 697
TaaI ACNGT 3 cut(s) 86, 448, 833
TaiI ACGT 3 cut(s) 60, 222, 879
TaqI TCGA 6 cut(s) 88, 297, 361, 579, 855, 897
TasI AATT 7 cut(s) 183, 197, 299, 425, 655, 902, 1127
TatI WGTACW 1 cut(s) 1157
TfiI GAWTC 1 cut(s) 235
Tru1I TTAA 2 cut(s) 195, 461
Tru9I TTAA 2 cut(s) 195, 461
TscAI CASTG 5 cut(s) 474, 712, 838, 1014, 1138
TseFI GTSAC 3 cut(s) 506, 877, 959
TseI GCWGC 4 cut(s) 116, 836, 1023, 1110
Tsp45I GTSAC 3 cut(s) 506, 877, 959
TspDTI ATGAA 4 cut(s) 17, 100, 452, 884
TspGWI ACGGA 1 cut(s) 275
TspRI CASTG 5 cut(s) 474, 712, 838, 1014, 1138
VpaK11BI GGWCC 3 cut(s) 262, 689, 1072
XapI RAATTY 5 cut(s) 197, 299, 425, 655, 1127
XceI RCATGY 1 cut(s) 135
XhoI CTCGAG 1 cut(s) 360
XmiI GTMKAC 2 cut(s) 140, 597
XmnI GAANNNNTTC 1 cut(s) 1062
XspI CTAG 3 cut(s) 417, 591, 719
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.