Rroxscaffold_7G00187250

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0014404
Physical Location & Seq
Forward (+)
26645012 .. 26650801
5790 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00187250.1

Sequence Viewer

Length: 1524 bp
ATGTCACTGAGAATCAAAGTGGTGGTGGACAAGTTTGTACAGGAGCTCAAGGAAGCTTTGGATGCGGATATTCAAGACAGAATCATGAAGGAGAGGAGATGCAGAGCTACATCGAAGAACGCGAACGCGAAGTCGCCGAGCGCGAAGCTGCTTGGAAAGCCGAGCTCTCTCGCCGTGAGGAACCCAGTGGAGGCAGAAATTGCCAGACAAGAAGCAAGGCTGAAAATAGAGAAGGAAAACCTTGAGAAAGAGAAAAGTGTTCTGATGGGGACTGCATCAAATCAAGATAACCAAGATGGAGCTCTTGAAATCACTGTAAGTGGTGAGAAGTATCGATGTCTTAGAGAAAGAGGAGCAGGTTCAGGCTTGCAGACCATCAATGTAGATCGCAAGGCCAAAAGCTCTCTTCTTTTTAATGTCAAGAGCTTTGGTGCCAAAGCAGATGGCCAAACCGATGACAGCAAAGCCTTCACAGCAGCATGGAAAGAGGCGTGCCAGTCCAAAGGTAGAGTCCACCTAATAATCCCAAGAGGAACATACATGATTGGGCCTCTTAAGTTTTCAGGCCCCTGCCAAAACGTATCGGCTCTAAATCTTCGAGGTTACTTGAAGGCTACAGCGGAGTTGTCCAAGTATGAATTCGGTGGCGGTTGGATTGAATTTGCTTGGATGAAGGGGCTAACCTTGACCGGAGGCGGAACCTTTGATGGTCAAGGTGCTAAAGCCTGGCCTCACAATAAGTGCTCTAAAGACTCCAATTGCAAACTTCTTCCAACTAGTCTGAAATTTGTTGCAATGAATAAGACAGTGATCCGGGGCATATCTTCGGTGAACAGCAAGTTCTTTCATATAGCTGTAGTGGAATGTCACAACTTCAAAGGTAGTAAACTCAAGATTCAAGCCCCAGAAACTAGTCCTAATACTGATGGAATCCACATTGAACGAAGCTCGAGTGTGTACTTCTCTTCGTCACACATTGCTACTGGTGATGACTGCATCTCAGTTGGACAAGGGAACTCCCAAGTCACCATAACTAGCATTGCTTGTGGACCTGGTCATGGCATCAGTGTGGGAAGTTTGGGAAAATATCCGAATGAAGGAGATGTTAGTGGGCTTGTGGTCAGAGACTGCACCATTTCAGGTACCACAAATGGTGTTAGGATCAAGACATGGGCTAACTCTCCAGGTAGAAGTGCAGCCACCAACATGACATTCACAAACATCATCATGAACAATGTGACTAACCCCATCATAATCGATCAGACATATTGTCCATTCACGTCTTGCTCTACTAAGGTGCCATCAAAAGTGAAGCTGAGTGACATTTACTTCAAGAACATAAGAGGGACCTCGTCGTCTCAAGTAGCGGTTGCACTTGAATGTAGCAAAGGGATTCCATGCCAGAACATTTACCTTGAAGATGTTCATCTGGACTTGTCATCTGGGAAGAAGGAAGCCACTTCAACTTGCAGTAATGTTAAAGCCAAATTCATTGGCACCCAAATCCCACCACCATGTACTTGA

Protein Analysis

507

Amino Acids

54.67

Weight (kDa)

9.31

Isoelectric Point (pI)

35.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pect-lyase_RHGA_epim PF12708 138 - 182 8.1e-07 Rhamnogalacturonase A/epimerase, pectate lyase-like
Glyco_hydro_28 PF00295 165 - 496 8.1e-95 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014404)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G18180
fragaria_vesca FvH4_2g16950
malus_domestica MD05G1141300.v1.1 MD05G1142200.v1.1
prunus_persica Prupe.8G181300_v2.0.a1
pyrus_communis pycom05g13670
rosa_chinensis RchiOBHm_Chr6g0281151
rosa_laevigata RLG00000012990
rosa_multiflora Rmu_co8202118.1_g000001 Rmu_sc0035236.1_g000002
rosa_roxburghii Rroxscaffold_7G00187250
rosa_rugosa Rorug06G0139300
rosa_samantha Rh6AG251100 Rh6BG254300 Rh6CG253700 Rh6DG245700
rosa_wichuraiana Rw6G021800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1354
Acc36I ACCTGC 1 cut(s) 347
Acc65I GGTACC 1 cut(s) 1142
AccB1I GGYRCC 4 cut(s) 431, 1142, 1297, 1496
AccII CGCG 3 cut(s) 122, 128, 143
AciI CCGC 5 cut(s) 65, 620, 648, 696, 1367
AclWI GGATC 2 cut(s) 805, 1169
AcoI YGGCCR 1 cut(s) 445
AcsI RAATTY 4 cut(s) 638, 659, 785, 1487
AfaI GTAC 4 cut(s) 39, 959, 1144, 1519
AfiI CCNNNNNNNGG 3 cut(s) 190, 1058, 1097
AflII CTTAAG 1 cut(s) 554
AhdI GACNNNNNGTC 1 cut(s) 1269
AhlI ACTAGT 2 cut(s) 776, 911
AjiI CACGTC 1 cut(s) 1281
AjnI CCWGG 3 cut(s) 725, 1051, 1183
AjuI GAANNNNNNNTTGG 4 cut(s) 623, 655, 1196, 1228
Alw21I GWGCWC 4 cut(s) 48, 167, 304, 746
Alw26I GTCTC 2 cut(s) 1119, 1362
AlwI GGATC 2 cut(s) 805, 1169
AlwNI CAGNNNCTG 1 cut(s) 1128
Ama87I CYCGRG 1 cut(s) 949
AoxI GGCC 5 cut(s) 393, 445, 548, 565, 728
ApeKI GCWGC 3 cut(s) 148, 476, 1196
ApoI RAATTY 4 cut(s) 638, 659, 785, 1487
ArsI GACNNNNNNTTYG 4 cut(s) 116, 148, 495, 527
Asp700I GAANNNNTTC 1 cut(s) 1422
Asp718I GGTACC 1 cut(s) 1142
AspLEI GCGC 1 cut(s) 143
AspS9I GGNCC 4 cut(s) 548, 566, 1049, 1347
AsuC2I CCSGG 1 cut(s) 815
AsuHPI GGTGA 4 cut(s) 335, 841, 998, 1018
AvaI CYCGRG 1 cut(s) 949
AvaII GGWCC 2 cut(s) 1049, 1347
BalI TGGCCA 1 cut(s) 447
BanI GGYRCC 4 cut(s) 431, 1142, 1297, 1496
BanII GRGCYC 3 cut(s) 48, 167, 304
Bbv12I GWGCWC 4 cut(s) 48, 167, 304, 746
BbvI GCAGC 3 cut(s) 135, 488, 1208
BccI CCATC 8 cut(s) 259, 290, 383, 437, 701, 920, 1256, 1309
BceAI ACGGC 1 cut(s) 158
BciT130I CCWGG 3 cut(s) 727, 1053, 1185
BcnI CCSGG 1 cut(s) 815
BcoDI GTCTC 2 cut(s) 1119, 1362
BcuI ACTAGT 2 cut(s) 776, 911
BfaI CTAG 3 cut(s) 777, 912, 1035
BfmI CTRYAG 2 cut(s) 615, 855
BfrI CTTAAG 1 cut(s) 554
BfuAI ACCTGC 1 cut(s) 347
BisI GCNGC 3 cut(s) 149, 477, 1197
BlsI GCNGC 3 cut(s) 150, 478, 1198
Bme1390I CCNGG 4 cut(s) 727, 815, 1053, 1185
Bme18I GGWCC 2 cut(s) 1049, 1347
BmeRI GACNNNNNGTC 1 cut(s) 1269
BmeT110I CYCGRG 1 cut(s) 949
BmgBI CACGTC 1 cut(s) 1281
BmgT120I GGNCC 4 cut(s) 548, 566, 1049, 1347
BmiI GGNNCC 8 cut(s) 182, 433, 568, 700, 1144, 1299, 1348, 1498
BmrFI CCNGG 4 cut(s) 727, 815, 1053, 1185
BmrI ACTGGG 1 cut(s) 179
BmsI GCATC 5 cut(s) 52, 89, 284, 1005, 1071
BmuI ACTGGG 1 cut(s) 179
BpmI CTGGAG 1 cut(s) 1167
BpuEI CTTGAG 4 cut(s) 32, 263, 875, 1344
BpuMI CCSGG 1 cut(s) 815
Bsa29I ATCGAT 2 cut(s) 334, 1257
BsaBI GATNNNNATC 1 cut(s) 1425
BsaJI CCNNGG 1 cut(s) 814
BsaWI WCCGGW 1 cut(s) 689
Bsc4I CCNNNNNNNGG 3 cut(s) 190, 1058, 1097
Bse1I ACTGG 3 cut(s) 185, 496, 988
Bse3DI GCAATG 3 cut(s) 801, 975, 1038
Bse8I GATNNNNATC 1 cut(s) 1425
BseBI CCWGG 3 cut(s) 727, 1053, 1185
BseCI ATCGAT 2 cut(s) 334, 1257
BseDI CCNNGG 1 cut(s) 814
BseGI GGATG 2 cut(s) 67, 675
BseJI GATNNNNATC 1 cut(s) 1425
BseLI CCNNNNNNNGG 3 cut(s) 190, 1058, 1097
BseMI GCAATG 3 cut(s) 801, 975, 1038
BseMII CTCAG 2 cut(s) 1014, 1307
BseNI ACTGG 3 cut(s) 185, 496, 988
BseRI GAGGAG 2 cut(s) 109, 366
BseXI GCAGC 3 cut(s) 135, 488, 1208
BsgI GTGCAG 2 cut(s) 1114, 1215
Bsh1236I CGCG 3 cut(s) 122, 128, 143
BshFI GGCC 5 cut(s) 395, 447, 550, 567, 730
BshNI GGYRCC 4 cut(s) 431, 1142, 1297, 1496
BshVI ATCGAT 2 cut(s) 334, 1257
BsiHKAI GWGCWC 4 cut(s) 48, 167, 304, 746
BsiHKCI CYCGRG 1 cut(s) 949
BsiSI CCGG 2 cut(s) 690, 814
BslFI GGGAC 2 cut(s) 283, 1360
BslI CCNNNNNNNGG 3 cut(s) 190, 1058, 1097
BsmAI GTCTC 2 cut(s) 1119, 1362
BsmBI CGTCTC 1 cut(s) 1362
BsmFI GGGAC 2 cut(s) 283, 1360
BsnI GGCC 5 cut(s) 395, 447, 550, 567, 730
BsoBI CYCGRG 1 cut(s) 949
Bsp1286I GDGCHC 4 cut(s) 48, 167, 304, 746
Bsp1407I TGTACA 1 cut(s) 37
Bsp143I GATC 4 cut(s) 385, 810, 1161, 1258
BspACI CCGC 5 cut(s) 65, 620, 648, 696, 1367
BspANI GGCC 5 cut(s) 395, 447, 550, 567, 730
BspCNI CTCAG 2 cut(s) 1013, 1308
BspDI ATCGAT 2 cut(s) 334, 1257
BspFNI CGCG 3 cut(s) 122, 128, 143
BspHI TCATGA 2 cut(s) 84, 1227
BspLI GGNNCC 8 cut(s) 182, 433, 568, 700, 1144, 1299, 1348, 1498
BspMI ACCTGC 1 cut(s) 347
BspPI GGATC 2 cut(s) 805, 1169
BspT107I GGYRCC 4 cut(s) 431, 1142, 1297, 1496
BspTI CTTAAG 1 cut(s) 554
BsrDI GCAATG 3 cut(s) 801, 975, 1038
BsrGI TGTACA 1 cut(s) 37
BsrI ACTGG 3 cut(s) 185, 496, 988
BssECI CCNNGG 1 cut(s) 814
BssMI GATC 4 cut(s) 385, 810, 1161, 1258
Bst2UI CCWGG 3 cut(s) 727, 1053, 1185
Bst4CI ACNGT 2 cut(s) 316, 808
Bst6I CTCTTC 2 cut(s) 411, 970
BstAFI CTTAAG 1 cut(s) 554
BstAPI GCANNNNNTGC 1 cut(s) 200
BstAUI TGTACA 1 cut(s) 37
BstC8I GCNNGC 2 cut(s) 368, 493
BstDEI CTNAG 5 cut(s) 8, 341, 1000, 1293, 1316
BstF5I GGATG 2 cut(s) 67, 675
BstFNI CGCG 3 cut(s) 122, 128, 143
BstHHI GCGC 1 cut(s) 143
BstKTI GATC 4 cut(s) 388, 813, 1164, 1261
BstMAI GTCTC 2 cut(s) 1119, 1362
BstMBI GATC 4 cut(s) 385, 810, 1161, 1258
BstMWI GCNNNNNNNGC 4 cut(s) 62, 157, 200, 473
BstNI CCWGG 3 cut(s) 727, 1053, 1185
BstSCI CCNGG 4 cut(s) 725, 813, 1051, 1183
BstSFI CTRYAG 2 cut(s) 615, 855
BstUI CGCG 3 cut(s) 122, 128, 143
BstV1I GCAGC 3 cut(s) 135, 488, 1208
Bsu15I ATCGAT 2 cut(s) 334, 1257
BsuRI GGCC 5 cut(s) 395, 447, 550, 567, 730
BsuTUI ATCGAT 2 cut(s) 334, 1257
BtrI CACGTC 1 cut(s) 1281
BtsCI GGATG 2 cut(s) 67, 675
BtsIMutI CAGTG 5 cut(s) 5, 192, 312, 813, 1072
BveI ACCTGC 1 cut(s) 347
Cac8I GCNNGC 2 cut(s) 368, 493
CaiI CAGNNNCTG 1 cut(s) 1128
CciI TCATGA 2 cut(s) 84, 1227
CfoI GCGC 1 cut(s) 143
Cfr13I GGNCC 4 cut(s) 548, 566, 1049, 1347
ClaI ATCGAT 2 cut(s) 334, 1257
CsiI ACCWGGT 1 cut(s) 1051
Csp6I GTAC 4 cut(s) 38, 958, 1143, 1518
CviAII CATG 9 cut(s) 85, 480, 541, 1058, 1170, 1207, 1228, 1398, 1515
CviQI GTAC 4 cut(s) 38, 958, 1143, 1518
DdeI CTNAG 5 cut(s) 8, 341, 1000, 1293, 1316
DpnI GATC 4 cut(s) 387, 812, 1163, 1260
DpnII GATC 4 cut(s) 385, 810, 1161, 1258
DrdI GACNNNNNNGTC 1 cut(s) 1354
DriI GACNNNNNGTC 1 cut(s) 1269
DseDI GACNNNNNNGTC 1 cut(s) 1354
EaeI YGGCCR 1 cut(s) 445
Eam1104I CTCTTC 2 cut(s) 411, 970
Eam1105I GACNNNNNGTC 1 cut(s) 1269
EarI CTCTTC 2 cut(s) 411, 970
EciI GGCGGA 1 cut(s) 711
Ecl136II GAGCTC 3 cut(s) 46, 165, 302
Eco24I GRGCYC 3 cut(s) 48, 167, 304
Eco47I GGWCC 2 cut(s) 1049, 1347
Eco53kI GAGCTC 3 cut(s) 46, 165, 302
Eco88I CYCGRG 1 cut(s) 949
EcoICRI GAGCTC 3 cut(s) 46, 165, 302
EcoO109I RGGNCCY 2 cut(s) 566, 1347
EcoRI GAATTC 1 cut(s) 638
EcoRII CCWGG 3 cut(s) 725, 1051, 1183
EcoT38I GRGCYC 3 cut(s) 48, 167, 304
Esp3I CGTCTC 1 cut(s) 1362
FaeI CATG 9 cut(s) 88, 483, 544, 1061, 1173, 1210, 1231, 1401, 1518
FaqI GGGAC 2 cut(s) 283, 1360
FatI CATG 9 cut(s) 84, 479, 540, 1057, 1169, 1206, 1227, 1397, 1514
Fnu4HI GCNGC 3 cut(s) 149, 477, 1197
FokI GGATG 2 cut(s) 74, 682
FriOI GRGCYC 3 cut(s) 48, 167, 304
Fsp4HI GCNGC 3 cut(s) 149, 477, 1197
FspBI CTAG 3 cut(s) 777, 912, 1035
GlaI GCGC 1 cut(s) 142
GluI GCNGC 3 cut(s) 149, 477, 1197
GsuI CTGGAG 1 cut(s) 1167
HaeIII GGCC 5 cut(s) 395, 447, 550, 567, 730
HapII CCGG 2 cut(s) 690, 814
HhaI GCGC 1 cut(s) 143
Hin1II CATG 9 cut(s) 88, 483, 544, 1061, 1173, 1210, 1231, 1401, 1518
Hin6I GCGC 1 cut(s) 141
HinP1I GCGC 1 cut(s) 141
HindIII AAGCTT 1 cut(s) 54
HinfI GANTC 7 cut(s) 12, 81, 510, 752, 895, 930, 1393
HpaII CCGG 2 cut(s) 690, 814
HphI GGTGA 4 cut(s) 335, 841, 998, 1018
Hpy166II GTNNAC 6 cut(s) 28, 514, 832, 887, 958, 1049
Hpy188I TCNGA 5 cut(s) 264, 783, 1092, 1124, 1263
Hpy8I GTNNAC 6 cut(s) 28, 514, 832, 887, 958, 1049
Hpy99I CGWCG 1 cut(s) 1357
HpyAV CCTTC 7 cut(s) 82, 226, 478, 604, 667, 1091, 1444
HpyCH4III ACNGT 2 cut(s) 316, 808
HpyCH4IV ACGT 2 cut(s) 579, 1280
HpyF10VI GCNNNNNNNGC 4 cut(s) 62, 157, 200, 473
HpyF3I CTNAG 5 cut(s) 8, 341, 1000, 1293, 1316
HpySE526I ACGT 2 cut(s) 579, 1280
Hsp92II CATG 9 cut(s) 88, 483, 544, 1061, 1173, 1210, 1231, 1401, 1518
HspAI GCGC 1 cut(s) 141
KpnI GGTACC 1 cut(s) 1146
Kzo9I GATC 4 cut(s) 385, 810, 1161, 1258
LmnI GCTCC 3 cut(s) 43, 299, 353
Lsp1109I GCAGC 3 cut(s) 135, 488, 1208
LweI GCATC 5 cut(s) 52, 89, 284, 1005, 1071
MabI ACCWGGT 1 cut(s) 1051
MaeI CTAG 3 cut(s) 777, 912, 1035
MaeII ACGT 2 cut(s) 579, 1280
MaeIII GTNAC 7 cut(s) 3, 602, 866, 969, 1024, 1237, 1319
MalI GATC 4 cut(s) 387, 812, 1163, 1260
MboI GATC 4 cut(s) 385, 810, 1161, 1258
MboII GAAGA 8 cut(s) 127, 398, 587, 761, 816, 957, 1430, 1459
MfeI CAATTG 1 cut(s) 757
MhlI GDGCHC 4 cut(s) 48, 167, 304, 746
MlsI TGGCCA 1 cut(s) 447
MluCI AATT 6 cut(s) 198, 638, 659, 757, 785, 1487
MluNI TGGCCA 1 cut(s) 447
MlyI GAGTC 2 cut(s) 519, 746
MmeI TCCRAC 3 cut(s) 632, 797, 985
Mox20I TGGCCA 1 cut(s) 447
MroXI GAANNNNTTC 1 cut(s) 1422
MscI TGGCCA 1 cut(s) 447
MseI TTAA 3 cut(s) 414, 555, 1479
MslI CAYNNNNRTG 5 cut(s) 1067, 1205, 1226, 1378, 1513
Msp20I TGGCCA 1 cut(s) 447
MspA1I CMGCKG 1 cut(s) 620
MspCI CTTAAG 1 cut(s) 554
MspI CCGG 2 cut(s) 690, 814
MspR9I CCNGG 4 cut(s) 727, 815, 1053, 1185
MunI CAATTG 1 cut(s) 757
MvaI CCWGG 3 cut(s) 727, 1053, 1185
MvnI CGCG 3 cut(s) 122, 128, 143
MwoI GCNNNNNNNGC 4 cut(s) 62, 157, 200, 473
NciI CCSGG 1 cut(s) 815
NdeII GATC 4 cut(s) 385, 810, 1161, 1258
NlaIII CATG 9 cut(s) 88, 483, 544, 1061, 1173, 1210, 1231, 1401, 1518
NlaIV GGNNCC 8 cut(s) 182, 433, 568, 700, 1144, 1299, 1348, 1498
NmeAIII GCCGAG 2 cut(s) 162, 186
NmuCI GTSAC 6 cut(s) 3, 866, 969, 1024, 1237, 1319
PaeR7I CTCGAG 1 cut(s) 949
PagI TCATGA 2 cut(s) 84, 1227
PcsI WCGNNNNNNNCGW 2 cut(s) 119, 140
PdmI GAANNNNTTC 1 cut(s) 1422
PfeI GAWTC 5 cut(s) 12, 81, 895, 930, 1393
PflFI GACNNNGTC 2 cut(s) 1053, 1351
PkrI GCNGC 3 cut(s) 150, 478, 1198
PleI GAGTC 2 cut(s) 518, 746
PpsI GAGTC 2 cut(s) 518, 746
PpuMI RGGWCCY 1 cut(s) 1347
Psp124BI GAGCTC 3 cut(s) 48, 167, 304
Psp5II RGGWCCY 1 cut(s) 1347
Psp6I CCWGG 3 cut(s) 725, 1051, 1183
PspGI CCWGG 3 cut(s) 725, 1051, 1183
PspN4I GGNNCC 8 cut(s) 182, 433, 568, 700, 1144, 1299, 1348, 1498
PspPI GGNCC 4 cut(s) 548, 566, 1049, 1347
PspPPI RGGWCCY 1 cut(s) 1347
PspXI VCTCGAGB 1 cut(s) 949
PstNI CAGNNNCTG 1 cut(s) 1128
PsyI GACNNNGTC 2 cut(s) 1053, 1351
RsaI GTAC 4 cut(s) 39, 959, 1144, 1519
RsaNI GTAC 4 cut(s) 38, 958, 1143, 1518
RseI CAYNNNNRTG 5 cut(s) 1067, 1205, 1226, 1378, 1513
SacI GAGCTC 3 cut(s) 48, 167, 304
SaqAI TTAA 3 cut(s) 414, 555, 1479
SatI GCNGC 3 cut(s) 149, 477, 1197
Sau3AI GATC 4 cut(s) 385, 810, 1161, 1258
Sau96I GGNCC 4 cut(s) 548, 566, 1049, 1347
SchI GAGTC 2 cut(s) 519, 746
ScrFI CCNGG 4 cut(s) 727, 815, 1053, 1185
SduI GDGCHC 4 cut(s) 48, 167, 304, 746
SexAI ACCWGGT 1 cut(s) 1051
SfaNI GCATC 5 cut(s) 52, 89, 284, 1005, 1071
SfcI CTRYAG 2 cut(s) 615, 855
Sfr274I CTCGAG 1 cut(s) 949
SinI GGWCC 2 cut(s) 1049, 1347
SlaI CTCGAG 1 cut(s) 949
SmiMI CAYNNNNRTG 5 cut(s) 1067, 1205, 1226, 1378, 1513
SmlI CTYRAG 6 cut(s) 47, 242, 554, 890, 949, 1359
SmoI CTYRAG 6 cut(s) 47, 242, 554, 890, 949, 1359
SpeI ACTAGT 2 cut(s) 776, 911
Sse9I AATT 6 cut(s) 198, 638, 659, 757, 785, 1487
SsiI CCGC 5 cut(s) 65, 620, 648, 696, 1367
SspMI CTAG 3 cut(s) 777, 912, 1035
SstI GAGCTC 3 cut(s) 48, 167, 304
StyD4I CCNGG 4 cut(s) 725, 813, 1051, 1183
TaaI ACNGT 2 cut(s) 316, 808
TaiI ACGT 2 cut(s) 582, 1283
TaqI TCGA 5 cut(s) 113, 334, 598, 950, 1257
TasI AATT 6 cut(s) 198, 638, 659, 757, 785, 1487
TatI WGTACW 3 cut(s) 37, 957, 1517
TfiI GAWTC 5 cut(s) 12, 81, 895, 930, 1393
Tru1I TTAA 3 cut(s) 414, 555, 1479
Tru9I TTAA 3 cut(s) 414, 555, 1479
TscAI CASTG 5 cut(s) 12, 192, 319, 813, 1072
TseFI GTSAC 6 cut(s) 3, 866, 969, 1024, 1237, 1319
TseI GCWGC 3 cut(s) 148, 476, 1196
Tsp45I GTSAC 6 cut(s) 3, 866, 969, 1024, 1237, 1319
TspDTI ATGAA 9 cut(s) 101, 651, 686, 812, 836, 1110, 1244, 1415, 1480
TspRI CASTG 5 cut(s) 12, 192, 319, 813, 1072
Tth111I GACNNNGTC 2 cut(s) 1053, 1351
Vha464I CTTAAG 1 cut(s) 554
VpaK11BI GGWCC 2 cut(s) 1049, 1347
XapI RAATTY 4 cut(s) 638, 659, 785, 1487
XhoI CTCGAG 1 cut(s) 949
XmnI GAANNNNTTC 1 cut(s) 1422
XspI CTAG 3 cut(s) 777, 912, 1035
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.