MD05G1141300.v1.1

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0014404
Physical Location & Seq
Reverse (-)
27172655 .. 27174497
1843 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1141300.v1.1.491

Sequence Viewer

Length: 1266 bp
ATGGGCAATTCGAGCCTCGAGTTCTTGGACGTCAGACTGCTCTTTTTGCTGTCCATGTTTTGCATGGCAAGTGGTTTCGGTAGAGGAGTCAAAGCAGACGGAAGAAGCCTCTTGCAGCATCACAGCAAGGCTAAGAGCTCTAATCTTTTCAACGTCAAGAGCTTTGGCGCCCGAGCAGACGGCCGAACTGATGACAGCGATGCATTCACGGCGGCATGGAAGGAGGCTTGCCAGTCCATAGGTAGAGTCCACCTTATAATACCGAAAGGAACTTACACGATCGGCCCTCTGAGATTCTCCGGCCCCTGCGTAAATGTTACGTCTCTGAATTTTCGTGTGAAGGGTTACTTGAAGGCTACAACTGACTTGCGCAAGTATGGATCGGGTAGTGGTTGGATTGAATTCGCGTGGATGAAAGGGCTAACCTTGACCGGAGGTGGAACCTTTGATGGTCGAGGGGCTCACGCCTGGCCTCACAACAAGTGCATCACCAACTCCAACTGCAAACTTCTTCCAACTAACTTGAAATTTGTGGCACTTAACAAGACAGTTATCCGCGGCTTAACTTCCGTGAACAGCAAGTTCTTTCACATAGCTCTTGTAGAATGTCACAACTTCAAAGGTAGCAGGCTCAAGATTTCAGCGCCCGAAACTAGCCCTAACACAGATGGAATCCACATCGAGCGAAGCTCTAGCATCTACTTCTCTTCGTCGCGCATTGCTACTGGTGATGACTGCATCTCAGTTGGACAAGGAAACTCTCAGGTCACCATAACAAGCATCACTTGTGGACCTGGTCATGGCATCAGTGTGGGAAGTCTAGGAAAATATCCGGACGAAAGAGATGTCAGCGGACTTGTAGTGAAGGACTGCACCATGACAGGCACCACCAATGGAATTAGGATCAAGACATGGGCTAACTCTCCAGGAAGCAGCGCTGCCACAAATATGACATTCGAAAACATTGTCATGAACAACGTTACTAATCCGATCATAATCGATCAGTCCTATTGTCCATTCACCGCTTGCACTTCTAAGGCACCATCTAGAGTGAAGCTGAGTGACATATACTTCAAGAACATAAGAGGAACTTCGTCTTCGGAGATGGCGGTGTTACTTGAATGCAGCAAAGGGATTCCATGCCAGAATATTTTCCTTGAAGATGTTCACTTGGATTTGTCATCCGGAGAGAAACTAGCCACTTCAAGTTGCAGTAATGTTAAAGCTAAATTTATTGGCACCCAAATCCCACCACCTTGTACCTAA

Protein Analysis

422

Amino Acids

45.28

Weight (kDa)

9.21

Isoelectric Point (pI)

35.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pect-lyase_RHGA_epim PF12708 50 - 94 7.3e-06 Rhamnogalacturonase A/epimerase, pectate lyase-like
Glyco_hydro_28 PF00295 77 - 410 1.6e-93 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014404)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G18180
fragaria_vesca FvH4_2g16950
malus_domestica MD05G1141300.v1.1 MD05G1142200.v1.1
prunus_persica Prupe.8G181300_v2.0.a1
pyrus_communis pycom05g13670
rosa_chinensis RchiOBHm_Chr6g0281151
rosa_laevigata RLG00000012990
rosa_multiflora Rmu_co8202118.1_g000001 Rmu_sc0035236.1_g000002
rosa_roxburghii Rroxscaffold_7G00187250
rosa_rugosa Rorug06G0139300
rosa_samantha Rh6AG251100 Rh6BG254300 Rh6CG253700 Rh6DG245700
rosa_wichuraiana Rw6G021800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 257
AatII GACGTC 1 cut(s) 33
Acc16I TGCGCA 1 cut(s) 371
AccB1I GGYRCC 4 cut(s) 167, 884, 1039, 1238
AccII CGCG 3 cut(s) 407, 558, 715
AccIII TCCGGA 2 cut(s) 832, 1184
AciI CCGC 6 cut(s) 212, 556, 558, 852, 1023, 1109
AclI AACGTT 1 cut(s) 978
AclWI GGATC 2 cut(s) 388, 911
AcoI YGGCCR 1 cut(s) 181
AcsI RAATTY 4 cut(s) 328, 401, 527, 1229
AcyI GRCGYC 2 cut(s) 30, 168
AfaI GTAC 1 cut(s) 1261
AfeI AGCGCT 1 cut(s) 937
AfiI CCNNNNNNNGG 1 cut(s) 800
AgsI TTSAA 9 cut(s) 151, 352, 401, 526, 619, 1075, 1121, 1160, 1206
AjnI CCWGG 3 cut(s) 467, 793, 925
AluBI AGCT 6 cut(s) 138, 162, 596, 690, 1057, 1226
AluI AGCT 6 cut(s) 138, 162, 596, 690, 1057, 1226
Alw21I GWGCWC 1 cut(s) 140
Alw26I GTCTC 1 cut(s) 327
AlwI GGATC 2 cut(s) 388, 911
Ama87I CYCGRG 2 cut(s) 17, 171
Aor13HI TCCGGA 2 cut(s) 832, 1184
Aor51HI AGCGCT 1 cut(s) 937
AoxI GGCC 4 cut(s) 181, 283, 301, 470
ApeKI GCWGC 4 cut(s) 115, 933, 938, 1125
ApoI RAATTY 4 cut(s) 328, 401, 527, 1229
ArsI GACNNNNNNTTYG 4 cut(s) 831, 863, 951, 983
Asp700I GAANNNNTTC 2 cut(s) 1151, 1164
AspLEI GCGC 5 cut(s) 170, 372, 646, 717, 938
AspS9I GGNCC 3 cut(s) 284, 302, 791
AsuHPI GGTGA 4 cut(s) 481, 740, 760, 1012
AsuII TTCGAA 1 cut(s) 957
AvaI CYCGRG 2 cut(s) 17, 171
AvaII GGWCC 1 cut(s) 791
BanI GGYRCC 4 cut(s) 167, 884, 1039, 1238
BanII GRGCYC 2 cut(s) 140, 463
BbsI GAAGAC 1 cut(s) 1089
Bbv12I GWGCWC 1 cut(s) 140
BbvI GCAGC 4 cut(s) 127, 925, 945, 1137
BccI CCATC 4 cut(s) 443, 662, 1051, 1099
BceAI ACGGC 2 cut(s) 196, 225
BciT130I CCWGG 3 cut(s) 469, 795, 927
BcoDI GTCTC 1 cut(s) 327
BfaI CTAG 5 cut(s) 654, 693, 821, 1047, 1196
BfoI RGCGCY 3 cut(s) 171, 647, 939
BisI GCNGC 6 cut(s) 116, 213, 559, 934, 939, 1126
BlsI GCNGC 6 cut(s) 117, 214, 560, 935, 940, 1127
Bme1390I CCNGG 3 cut(s) 469, 795, 927
Bme18I GGWCC 1 cut(s) 791
BmeT110I CYCGRG 2 cut(s) 17, 171
BmgT120I GGNCC 3 cut(s) 284, 302, 791
BmiI GGNNCC 6 cut(s) 169, 304, 442, 886, 1041, 1240
BmrFI CCNGG 3 cut(s) 469, 795, 927
BmsI GCATC 7 cut(s) 127, 190, 495, 705, 747, 789, 813
BpiI GAAGAC 1 cut(s) 1089
BplI GAGNNNNNCTC 2 cut(s) 674, 706
BpmI CTGGAG 1 cut(s) 909
Bpu14I TTCGAA 1 cut(s) 957
BpuEI CTTGAG 1 cut(s) 617
Bsa29I ATCGAT 1 cut(s) 999
BsaBI GATNNNNATC 1 cut(s) 995
BsaHI GRCGYC 2 cut(s) 30, 168
BsaJI CCNNGG 1 cut(s) 556
BsaWI WCCGGW 3 cut(s) 431, 832, 1184
Bsc4I CCNNNNNNNGG 1 cut(s) 800
Bse1I ACTGG 2 cut(s) 232, 730
Bse3DI GCAATG 1 cut(s) 717
Bse8I GATNNNNATC 1 cut(s) 995
BseAI TCCGGA 2 cut(s) 832, 1184
BseBI CCWGG 3 cut(s) 469, 795, 927
BseCI ATCGAT 1 cut(s) 999
BseDI CCNNGG 1 cut(s) 556
BseGI GGATG 2 cut(s) 417, 1181
BseJI GATNNNNATC 1 cut(s) 995
BseLI CCNNNNNNNGG 1 cut(s) 800
BseMI GCAATG 1 cut(s) 717
BseMII CTCAG 4 cut(s) 281, 756, 776, 1049
BseNI ACTGG 2 cut(s) 232, 730
BseRI GAGGAG 1 cut(s) 99
BseX3I CGGCCG 1 cut(s) 181
BseXI GCAGC 4 cut(s) 127, 925, 945, 1137
BsgI GTGCAG 1 cut(s) 856
Bsh1236I CGCG 3 cut(s) 407, 558, 715
Bsh1285I CGRYCG 2 cut(s) 184, 282
BshFI GGCC 4 cut(s) 183, 285, 303, 472
BshNI GGYRCC 4 cut(s) 167, 884, 1039, 1238
BshVI ATCGAT 1 cut(s) 999
BsiEI CGRYCG 2 cut(s) 184, 282
BsiHKAI GWGCWC 1 cut(s) 140
BsiHKCI CYCGRG 2 cut(s) 17, 171
BsiSI CCGG 4 cut(s) 300, 432, 833, 1185
BslI CCNNNNNNNGG 1 cut(s) 800
BsmAI GTCTC 1 cut(s) 327
BsmBI CGTCTC 1 cut(s) 327
BsmI GAATGC 2 cut(s) 203, 1127
BsnI GGCC 4 cut(s) 183, 285, 303, 472
BsoBI CYCGRG 2 cut(s) 17, 171
Bsp119I TTCGAA 1 cut(s) 957
Bsp1286I GDGCHC 2 cut(s) 140, 463
Bsp13I TCCGGA 2 cut(s) 832, 1184
Bsp143I GATC 5 cut(s) 279, 380, 903, 990, 1000
BspACI CCGC 6 cut(s) 212, 556, 558, 852, 1023, 1109
BspANI GGCC 4 cut(s) 183, 285, 303, 472
BspCNI CTCAG 4 cut(s) 282, 755, 775, 1050
BspDI ATCGAT 1 cut(s) 999
BspEI TCCGGA 2 cut(s) 832, 1184
BspFNI CGCG 3 cut(s) 407, 558, 715
BspHI TCATGA 1 cut(s) 969
BspLI GGNNCC 6 cut(s) 169, 304, 442, 886, 1041, 1240
BspPI GGATC 2 cut(s) 388, 911
BspT104I TTCGAA 1 cut(s) 957
BspT107I GGYRCC 4 cut(s) 167, 884, 1039, 1238
BsrDI GCAATG 1 cut(s) 717
BsrI ACTGG 2 cut(s) 232, 730
BssECI CCNNGG 1 cut(s) 556
BssMI GATC 5 cut(s) 279, 380, 903, 990, 1000
BssNI GRCGYC 2 cut(s) 30, 168
Bst2UI CCWGG 3 cut(s) 469, 795, 927
Bst4CI ACNGT 1 cut(s) 550
Bst6I CTCTTC 1 cut(s) 712
BstACI GRCGYC 2 cut(s) 30, 168
BstBI TTCGAA 1 cut(s) 957
BstC8I GCNNGC 3 cut(s) 229, 629, 1027
BstDEI CTNAG 6 cut(s) 132, 290, 742, 762, 1035, 1058
BstDSI CCRYGG 1 cut(s) 556
BstEII GGTNACC 1 cut(s) 766
BstF5I GGATG 2 cut(s) 417, 1181
BstFNI CGCG 3 cut(s) 407, 558, 715
BstH2I RGCGCY 3 cut(s) 171, 647, 939
BstHHI GCGC 5 cut(s) 170, 372, 646, 717, 938
BstKTI GATC 5 cut(s) 282, 383, 906, 993, 1003
BstMAI GTCTC 1 cut(s) 327
BstMBI GATC 5 cut(s) 279, 380, 903, 990, 1000
BstMCI CGRYCG 2 cut(s) 184, 282
BstMWI GCNNNNNNNGC 3 cut(s) 12, 46, 209
BstNI CCWGG 3 cut(s) 469, 795, 927
BstPI GGTNACC 1 cut(s) 766
BstSCI CCNGG 3 cut(s) 467, 793, 925
BstUI CGCG 3 cut(s) 407, 558, 715
BstV1I GCAGC 4 cut(s) 127, 925, 945, 1137
BstV2I GAAGAC 1 cut(s) 1089
BstZI CGGCCG 1 cut(s) 181
Bsu15I ATCGAT 1 cut(s) 999
BsuRI GGCC 4 cut(s) 183, 285, 303, 472
BsuTUI ATCGAT 1 cut(s) 999
BtgI CCRYGG 1 cut(s) 556
BtgZI GCGATG 1 cut(s) 213
BtsCI GGATG 2 cut(s) 417, 1181
BtsIMutI CAGTG 1 cut(s) 814
Cac8I GCNNGC 3 cut(s) 229, 629, 1027
CciI TCATGA 1 cut(s) 969
CfoI GCGC 5 cut(s) 170, 372, 646, 717, 938
Cfr13I GGNCC 3 cut(s) 284, 302, 791
Cfr42I CCGCGG 1 cut(s) 559
ClaI ATCGAT 1 cut(s) 999
CsiI ACCWGGT 1 cut(s) 793
Csp6I GTAC 1 cut(s) 1260
CviAII CATG 8 cut(s) 55, 64, 216, 800, 877, 912, 970, 1140
CviQI GTAC 1 cut(s) 1260
DdeI CTNAG 6 cut(s) 132, 290, 742, 762, 1035, 1058
DinI GGCGCC 1 cut(s) 169
DpnI GATC 5 cut(s) 281, 382, 905, 992, 1002
DpnII GATC 5 cut(s) 279, 380, 903, 990, 1000
EaeI YGGCCR 1 cut(s) 181
EagI CGGCCG 1 cut(s) 181
Eam1104I CTCTTC 1 cut(s) 712
EarI CTCTTC 1 cut(s) 712
Ecl136II GAGCTC 1 cut(s) 138
EclXI CGGCCG 1 cut(s) 181
Eco24I GRGCYC 2 cut(s) 140, 463
Eco47I GGWCC 1 cut(s) 791
Eco47III AGCGCT 1 cut(s) 937
Eco52I CGGCCG 1 cut(s) 181
Eco53kI GAGCTC 1 cut(s) 138
Eco88I CYCGRG 2 cut(s) 17, 171
Eco91I GGTNACC 1 cut(s) 766
EcoICRI GAGCTC 1 cut(s) 138
EcoO65I GGTNACC 1 cut(s) 766
EcoRI GAATTC 1 cut(s) 401
EcoRII CCWGG 3 cut(s) 467, 793, 925
EcoT22I ATGCAT 1 cut(s) 205
EcoT38I GRGCYC 2 cut(s) 140, 463
EgeI GGCGCC 1 cut(s) 169
EheI GGCGCC 1 cut(s) 169
Esp3I CGTCTC 1 cut(s) 327
FaeI CATG 8 cut(s) 58, 67, 219, 803, 880, 915, 973, 1143
FalI AAGNNNNNCTT 6 cut(s) 332, 364, 769, 801, 1075, 1107
FatI CATG 8 cut(s) 54, 63, 215, 799, 876, 911, 969, 1139
Fnu4HI GCNGC 6 cut(s) 116, 213, 559, 934, 939, 1126
FokI GGATG 2 cut(s) 424, 1168
FriOI GRGCYC 2 cut(s) 140, 463
Fsp4HI GCNGC 6 cut(s) 116, 213, 559, 934, 939, 1126
FspBI CTAG 5 cut(s) 654, 693, 821, 1047, 1196
FspI TGCGCA 1 cut(s) 371
GlaI GCGC 5 cut(s) 169, 371, 645, 716, 937
GluI GCNGC 6 cut(s) 116, 213, 559, 934, 939, 1126
GsuI CTGGAG 1 cut(s) 909
HaeII RGCGCY 3 cut(s) 171, 647, 939
HaeIII GGCC 4 cut(s) 183, 285, 303, 472
HapII CCGG 4 cut(s) 300, 432, 833, 1185
HhaI GCGC 5 cut(s) 170, 372, 646, 717, 938
Hin1I GRCGYC 2 cut(s) 30, 168
Hin1II CATG 8 cut(s) 58, 67, 219, 803, 880, 915, 973, 1143
Hin6I GCGC 5 cut(s) 168, 370, 644, 715, 936
HinP1I GCGC 5 cut(s) 168, 370, 644, 715, 936
HinfI GANTC 5 cut(s) 87, 246, 294, 672, 1135
HpaII CCGG 4 cut(s) 300, 432, 833, 1185
HphI GGTGA 4 cut(s) 481, 740, 760, 1012
Hpy166II GTNNAC 4 cut(s) 250, 574, 791, 1168
Hpy188I TCNGA 5 cut(s) 35, 291, 327, 990, 1102
Hpy188III TCNNGA 8 cut(s) 157, 634, 833, 907, 970, 1047, 1075, 1185
Hpy8I GTNNAC 4 cut(s) 250, 574, 791, 1168
Hpy99I CGWCG 1 cut(s) 715
HpyAV CCTTC 4 cut(s) 214, 334, 346, 859
HpyCH4III ACNGT 1 cut(s) 550
HpyCH4IV ACGT 4 cut(s) 30, 153, 320, 978
HpyF10VI GCNNNNNNNGC 3 cut(s) 12, 46, 209
HpyF3I CTNAG 6 cut(s) 132, 290, 742, 762, 1035, 1058
HpySE526I ACGT 4 cut(s) 30, 153, 320, 978
Hsp92I GRCGYC 2 cut(s) 30, 168
Hsp92II CATG 8 cut(s) 58, 67, 219, 803, 880, 915, 973, 1143
HspAI GCGC 5 cut(s) 168, 370, 644, 715, 936
KasI GGCGCC 1 cut(s) 167
Kpn2I TCCGGA 2 cut(s) 832, 1184
KspI CCGCGG 1 cut(s) 559
Kzo9I GATC 5 cut(s) 279, 380, 903, 990, 1000
Lsp1109I GCAGC 4 cut(s) 127, 925, 945, 1137
LweI GCATC 7 cut(s) 127, 190, 495, 705, 747, 789, 813
MabI ACCWGGT 1 cut(s) 793
MaeI CTAG 5 cut(s) 654, 693, 821, 1047, 1196
MaeII ACGT 4 cut(s) 30, 153, 320, 978
MaeIII GTNAC 7 cut(s) 316, 344, 608, 766, 979, 1061, 1113
MalI GATC 5 cut(s) 281, 382, 905, 992, 1002
MboI GATC 5 cut(s) 279, 380, 903, 990, 1000
MboII GAAGA 5 cut(s) 114, 503, 699, 1089, 1172
MhlI GDGCHC 2 cut(s) 140, 463
MluCI AATT 6 cut(s) 7, 328, 401, 527, 897, 1229
Mly113I GGCGCC 1 cut(s) 168
MlyI GAGTC 2 cut(s) 96, 255
MmeI TCCRAC 4 cut(s) 374, 522, 539, 727
MnlI CCTC 9 cut(s) 26, 77, 119, 217, 297, 428, 449, 483, 1079
Mph1103I ATGCAT 1 cut(s) 205
MroI TCCGGA 2 cut(s) 832, 1184
MroXI GAANNNNTTC 2 cut(s) 1151, 1164
MseI TTAA 3 cut(s) 540, 563, 1221
MslI CAYNNNNRTG 3 cut(s) 809, 947, 968
MspA1I CMGCKG 2 cut(s) 558, 852
MspI CCGG 4 cut(s) 300, 432, 833, 1185
MspR9I CCNGG 3 cut(s) 469, 795, 927
Mva1269I GAATGC 2 cut(s) 203, 1127
MvaI CCWGG 3 cut(s) 469, 795, 927
MvnI CGCG 3 cut(s) 407, 558, 715
MwoI GCNNNNNNNGC 3 cut(s) 12, 46, 209
NarI GGCGCC 1 cut(s) 168
NdeII GATC 5 cut(s) 279, 380, 903, 990, 1000
NlaIII CATG 8 cut(s) 58, 67, 219, 803, 880, 915, 973, 1143
NlaIV GGNNCC 6 cut(s) 169, 304, 442, 886, 1041, 1240
NmuCI GTSAC 3 cut(s) 608, 766, 1061
NsbI TGCGCA 1 cut(s) 371
NsiI ATGCAT 1 cut(s) 205
NspV TTCGAA 1 cut(s) 957
PaeR7I CTCGAG 1 cut(s) 17
PagI TCATGA 1 cut(s) 969
PctI GAATGC 2 cut(s) 203, 1127
PdmI GAANNNNTTC 2 cut(s) 1151, 1164
PfeI GAWTC 3 cut(s) 294, 672, 1135
PflFI GACNNNGTC 1 cut(s) 795
PfoI TCCNGGA 1 cut(s) 925
PkrI GCNGC 6 cut(s) 117, 214, 560, 935, 940, 1127
Ple19I CGATCG 1 cut(s) 282
PleI GAGTC 2 cut(s) 95, 254
PluTI GGCGCC 1 cut(s) 171
PpsI GAGTC 2 cut(s) 95, 254
PsiI TTATAA 1 cut(s) 257
Psp124BI GAGCTC 1 cut(s) 140
Psp1406I AACGTT 1 cut(s) 978
Psp6I CCWGG 3 cut(s) 467, 793, 925
PspEI GGTNACC 1 cut(s) 766
PspGI CCWGG 3 cut(s) 467, 793, 925
PspN4I GGNNCC 6 cut(s) 169, 304, 442, 886, 1041, 1240
PspPI GGNCC 3 cut(s) 284, 302, 791
PspXI VCTCGAGB 1 cut(s) 17
PsyI GACNNNGTC 1 cut(s) 795
PvuI CGATCG 1 cut(s) 282
RsaI GTAC 1 cut(s) 1261
RsaNI GTAC 1 cut(s) 1260
RseI CAYNNNNRTG 3 cut(s) 809, 947, 968
SacI GAGCTC 1 cut(s) 140
SacII CCGCGG 1 cut(s) 559
SaqAI TTAA 3 cut(s) 540, 563, 1221
SatI GCNGC 6 cut(s) 116, 213, 559, 934, 939, 1126
Sau3AI GATC 5 cut(s) 279, 380, 903, 990, 1000
Sau96I GGNCC 3 cut(s) 284, 302, 791
SchI GAGTC 2 cut(s) 96, 255
ScrFI CCNGG 3 cut(s) 469, 795, 927
SduI GDGCHC 2 cut(s) 140, 463
SexAI ACCWGGT 1 cut(s) 793
SfaNI GCATC 7 cut(s) 127, 190, 495, 705, 747, 789, 813
SfoI GGCGCC 1 cut(s) 169
Sfr274I CTCGAG 1 cut(s) 17
Sfr303I CCGCGG 1 cut(s) 559
SfuI TTCGAA 1 cut(s) 957
SgrBI CCGCGG 1 cut(s) 559
SinI GGWCC 1 cut(s) 791
SlaI CTCGAG 1 cut(s) 17
SmiMI CAYNNNNRTG 3 cut(s) 809, 947, 968
SmlI CTYRAG 2 cut(s) 17, 632
SmoI CTYRAG 2 cut(s) 17, 632
Sse9I AATT 6 cut(s) 7, 328, 401, 527, 897, 1229
SsiI CCGC 6 cut(s) 212, 556, 558, 852, 1023, 1109
SspDI GGCGCC 1 cut(s) 167
SspI AATATT 1 cut(s) 1150
SspMI CTAG 5 cut(s) 654, 693, 821, 1047, 1196
SstI GAGCTC 1 cut(s) 140
StyD4I CCNGG 3 cut(s) 467, 793, 925
TaaI ACNGT 1 cut(s) 550
TaiI ACGT 4 cut(s) 33, 156, 323, 981
TaqI TCGA 6 cut(s) 11, 18, 454, 681, 957, 999
TasI AATT 6 cut(s) 7, 328, 401, 527, 897, 1229
TauI GCSGC 2 cut(s) 215, 561
TfiI GAWTC 3 cut(s) 294, 672, 1135
Tru1I TTAA 3 cut(s) 540, 563, 1221
Tru9I TTAA 3 cut(s) 540, 563, 1221
TscAI CASTG 1 cut(s) 814
TseFI GTSAC 3 cut(s) 608, 766, 1061
TseI GCWGC 4 cut(s) 115, 933, 938, 1125
Tsp45I GTSAC 3 cut(s) 608, 766, 1061
TspDTI ATGAA 2 cut(s) 428, 986
TspGWI ACGGA 2 cut(s) 114, 559
TspRI CASTG 1 cut(s) 814
Tth111I GACNNNGTC 1 cut(s) 795
VpaK11BI GGWCC 1 cut(s) 791
XapI RAATTY 4 cut(s) 328, 401, 527, 1229
XbaI TCTAGA 1 cut(s) 1046
XcmI CCANNNNNNNNNTGG 1 cut(s) 61
XhoI CTCGAG 1 cut(s) 17
XmnI GAANNNNTTC 2 cut(s) 1151, 1164
XspI CTAG 5 cut(s) 654, 693, 821, 1047, 1196
ZraI GACGTC 1 cut(s) 31
Zsp2I ATGCAT 1 cut(s) 205
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.