AT4G18450

DNA-binding domain in plant proteins such as APETALA2 and EREBPs

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0015404
Physical Location & Seq
Reverse (-)
10190231 .. 10191252
1022 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G18450.1

Sequence Viewer

Length: 912 bp
ATGGCTTTTGGCAATATCCAAGAACTAGACGGCGAGATCCTAAAGAACGTTTGGGCGAATTACATCGGAACACCACAAACCGATACAAGATCAATTCAAGTTCCAGAAGTTTCTAGAACTTGGGAAGCGTTGCCTACCCTTGATGACATACCAGAAGGTTCTAGAGAAATGCTTCAAAGCCTAGATATGTCGACGGAGGACCAGGAATGGACAGAGATTCTCGATGCTATTGCTTCTTTCCCAAACAAAACCAATCATGATCCATTAACCAACCCTACCATTGATTCATGTTCTTTGTCTTCTCGGGTTTCTTGCAAAACAAGAAAATACAGGGGAGTACGGAAGCGTCCGTGGGGGAAATTTGCAGCCGAAATCAGGGATTCGACGAGAAACGGTGTTAGGGTTTGGCTCGGAACGTTCCAAACTGCAGAGGAAGCAGCTATGGCTTACGATAAAGCCGCGGTTAGAATTAGAGGTACTCAAAAAGCTCACACAAATTTTCAGCTCGAAACAGTTATAAAAGCTATGGAAATGGATTGCAACCCAAACTACTACCGGATGAACAACTCAAATACGTCCGATCCATTAAGAAGCAGCCGCAAAATCGGATTGAGAACAGGAAAAGAGGCGGTTAAGGCTTATGATGAAGTCGTTGATGGGATGGTTGAAAACCATTGTGCCCTTAGCTATTGTTCAACTAAGGAGCACTCGGAGACTCGTGGTTTGCGTGGGAGTGAAGAAACTTGGTTCGATTTAAGAAAGAGACGAAGGAGTAATGAAGATTCTATGTGTCAAGAAGTTGAAATGCAGAAGACGGTTACTGGAGAAGAGACAGTATGTGATGTGTTTGGTTTGTTTGAGTTTGAGGATTTGGGAAGTGATTATTTGGAGACGTTATTATCTTCTTTTTGA

Protein Analysis

303

Amino Acids

34.49

Weight (kDa)

5.07

Isoelectric Point (pI)

44.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 109 - 159 1.5e-12 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015404)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G18450
fragaria_vesca FvH4_4g10480
malus_domestica MD05G1306800.v1.1 MD10G1286200.v1.1
prunus_persica Prupe.4G055600_v2.0.a1
pyrus_communis pycom10g23890
rosa_chinensis RchiOBHm_Chr5g0009741
rosa_laevigata RLG00000031690
rosa_multiflora Rmu_sc0000410.1_g000002
rosa_roxburghii Rroxscaffold_1G00066480
rosa_rugosa Rorug04G0445400
rosa_samantha Rh5AG076900 Rh5BG072200 Rh5CG084600 Rh5DG072600
rosa_wichuraiana Rw5G007090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 518
AccI GTMKAC 1 cut(s) 191
AccII CGCG 1 cut(s) 461
AciI CCGC 4 cut(s) 459, 461, 598, 629
AclI AACGTT 2 cut(s) 48, 416
AclWI GGATC 3 cut(s) 31, 254, 575
AcsI RAATTY 2 cut(s) 359, 496
AfaI GTAC 2 cut(s) 339, 478
AfiI CCNNNNNNNGG 1 cut(s) 375
AgsI TTSAA 5 cut(s) 98, 176, 668, 696, 803
AjnI CCWGG 1 cut(s) 201
AluBI AGCT 5 cut(s) 440, 488, 505, 524, 687
AluI AGCT 5 cut(s) 440, 488, 505, 524, 687
Alw21I GWGCWC 1 cut(s) 708
Alw26I GTCTC 4 cut(s) 707, 757, 824, 884
AlwI GGATC 3 cut(s) 31, 254, 575
Ama87I CYCGRG 1 cut(s) 303
ApeKI GCWGC 3 cut(s) 365, 437, 594
ApoI RAATTY 2 cut(s) 359, 496
ArsI GACNNNNNNTTYG 2 cut(s) 706, 738
Asp700I GAANNNNTTC 1 cut(s) 171
AspS9I GGNCC 1 cut(s) 199
AvaI CYCGRG 1 cut(s) 303
AvaII GGWCC 1 cut(s) 199
BaeGI GKGCMC 1 cut(s) 682
BauI CACGAG 1 cut(s) 717
BbsI GAAGAC 2 cut(s) 291, 818
Bbv12I GWGCWC 1 cut(s) 708
BbvI GCAGC 3 cut(s) 377, 449, 606
BccI CCATC 2 cut(s) 650, 655
BceAI ACGGC 1 cut(s) 46
BcgI CGANNNNNNTGC 2 cut(s) 212, 246
BciT130I CCWGG 1 cut(s) 203
BcoDI GTCTC 4 cut(s) 707, 757, 824, 884
BfaI CTAG 4 cut(s) 26, 114, 162, 182
BfmI CTRYAG 1 cut(s) 426
BisI GCNGC 5 cut(s) 366, 438, 459, 595, 598
BlsI GCNGC 5 cut(s) 367, 439, 460, 596, 599
Bme1390I CCNGG 1 cut(s) 203
Bme18I GGWCC 1 cut(s) 199
BmeT110I CYCGRG 1 cut(s) 303
BmgT120I GGNCC 1 cut(s) 199
BmrFI CCNGG 1 cut(s) 203
BmsI GCATC 1 cut(s) 214
BpiI GAAGAC 2 cut(s) 291, 818
BpmI CTGGAG 1 cut(s) 843
Bpu10I CCTNAGC 1 cut(s) 683
BsaJI CCNNGG 2 cut(s) 350, 459
BsaWI WCCGGW 1 cut(s) 555
Bsc4I CCNNNNNNNGG 1 cut(s) 375
Bse1I ACTGG 1 cut(s) 826
BseBI CCWGG 1 cut(s) 203
BseDI CCNNGG 2 cut(s) 350, 459
BseGI GGATG 2 cut(s) 564, 666
BseLI CCNNNNNNNGG 1 cut(s) 375
BseNI ACTGG 1 cut(s) 826
BseSI GKGCMC 1 cut(s) 682
BseXI GCAGC 3 cut(s) 377, 449, 606
Bsh1236I CGCG 1 cut(s) 461
BsiHKAI GWGCWC 1 cut(s) 708
BsiHKCI CYCGRG 1 cut(s) 303
BsiSI CCGG 1 cut(s) 556
BslI CCNNNNNNNGG 1 cut(s) 375
BsmAI GTCTC 4 cut(s) 707, 757, 824, 884
BsmBI CGTCTC 2 cut(s) 757, 884
BsoBI CYCGRG 1 cut(s) 303
Bsp1286I GDGCHC 2 cut(s) 682, 708
Bsp143I GATC 4 cut(s) 36, 89, 259, 580
BspACI CCGC 4 cut(s) 459, 461, 598, 629
BspFNI CGCG 1 cut(s) 461
BspHI TCATGA 1 cut(s) 256
BspMAI CTGCAG 1 cut(s) 430
BspPI GGATC 3 cut(s) 31, 254, 575
BsrI ACTGG 1 cut(s) 826
BssECI CCNNGG 2 cut(s) 350, 459
BssMI GATC 4 cut(s) 36, 89, 259, 580
BssSI CACGAG 1 cut(s) 717
Bst2BI CACGAG 1 cut(s) 717
Bst2UI CCWGG 1 cut(s) 203
Bst4CI ACNGT 4 cut(s) 395, 514, 817, 835
Bst6I CTCTTC 1 cut(s) 822
BstDEI CTNAG 2 cut(s) 683, 699
BstDSI CCRYGG 2 cut(s) 350, 459
BstF5I GGATG 2 cut(s) 564, 666
BstFNI CGCG 1 cut(s) 461
BstKTI GATC 4 cut(s) 39, 92, 262, 583
BstMAI GTCTC 4 cut(s) 707, 757, 824, 884
BstMBI GATC 4 cut(s) 36, 89, 259, 580
BstMWI GCNNNNNNNGC 3 cut(s) 434, 443, 635
BstNI CCWGG 1 cut(s) 203
BstSCI CCNGG 1 cut(s) 201
BstSFI CTRYAG 1 cut(s) 426
BstSLI GKGCMC 1 cut(s) 682
BstUI CGCG 1 cut(s) 461
BstV1I GCAGC 3 cut(s) 377, 449, 606
BstV2I GAAGAC 2 cut(s) 291, 818
BstX2I RGATCY 1 cut(s) 36
BstYI RGATCY 1 cut(s) 36
BtgI CCRYGG 2 cut(s) 350, 459
BtsCI GGATG 2 cut(s) 564, 666
CciI TCATGA 1 cut(s) 256
Cfr13I GGNCC 1 cut(s) 199
Cfr42I CCGCGG 1 cut(s) 462
CseI GACGC 1 cut(s) 335
Csp6I GTAC 2 cut(s) 338, 477
CviAII CATG 2 cut(s) 257, 288
CviQI GTAC 2 cut(s) 338, 477
DdeI CTNAG 2 cut(s) 683, 699
DpnI GATC 4 cut(s) 38, 91, 261, 582
DpnII GATC 4 cut(s) 36, 89, 259, 580
Eam1104I CTCTTC 1 cut(s) 822
EarI CTCTTC 1 cut(s) 822
Eco47I GGWCC 1 cut(s) 199
Eco88I CYCGRG 1 cut(s) 303
EcoRII CCWGG 1 cut(s) 201
Esp3I CGTCTC 2 cut(s) 757, 884
FaeI CATG 2 cut(s) 260, 291
FatI CATG 2 cut(s) 256, 287
FblI GTMKAC 1 cut(s) 191
Fnu4HI GCNGC 5 cut(s) 366, 438, 459, 595, 598
FokI GGATG 2 cut(s) 571, 673
Fsp4HI GCNGC 5 cut(s) 366, 438, 459, 595, 598
FspBI CTAG 4 cut(s) 26, 114, 162, 182
GluI GCNGC 5 cut(s) 366, 438, 459, 595, 598
GsuI CTGGAG 1 cut(s) 843
HapII CCGG 1 cut(s) 556
HgaI GACGC 1 cut(s) 335
Hin1II CATG 2 cut(s) 260, 291
HincII GTYRAC 1 cut(s) 192
HindII GTYRAC 1 cut(s) 192
HinfI GANTC 5 cut(s) 217, 284, 380, 715, 782
HpaII CCGG 1 cut(s) 556
Hpy166II GTNNAC 1 cut(s) 192
Hpy188I TCNGA 5 cut(s) 68, 413, 580, 608, 712
Hpy188III TCNNGA 6 cut(s) 104, 114, 162, 221, 257, 794
Hpy8I GTNNAC 1 cut(s) 192
Hpy99I CGWCG 2 cut(s) 196, 388
HpyAV CCTTC 2 cut(s) 149, 762
HpyCH4III ACNGT 4 cut(s) 395, 514, 817, 835
HpyCH4IV ACGT 4 cut(s) 48, 416, 575, 893
HpyCH4V TGCA 5 cut(s) 315, 365, 428, 540, 808
HpyF10VI GCNNNNNNNGC 3 cut(s) 434, 443, 635
HpyF3I CTNAG 2 cut(s) 683, 699
HpySE526I ACGT 4 cut(s) 48, 416, 575, 893
Hsp92II CATG 2 cut(s) 260, 291
KspI CCGCGG 1 cut(s) 462
Kzo9I GATC 4 cut(s) 36, 89, 259, 580
LmnI GCTCC 1 cut(s) 703
LpnPI CCDG 9 cut(s) 117, 165, 188, 215, 316, 361, 569, 603, 807
Lsp1109I GCAGC 3 cut(s) 377, 449, 606
LweI GCATC 1 cut(s) 214
MaeI CTAG 4 cut(s) 26, 114, 162, 182
MaeII ACGT 4 cut(s) 48, 416, 575, 893
MaeIII GTNAC 1 cut(s) 817
MalI GATC 4 cut(s) 38, 91, 261, 582
MboI GATC 4 cut(s) 36, 89, 259, 580
MboII GAAGA 6 cut(s) 291, 749, 791, 823, 839, 894
MflI RGATCY 1 cut(s) 36
MhlI GDGCHC 2 cut(s) 682, 708
MluCI AATT 5 cut(s) 58, 93, 359, 468, 496
MlyI GAGTC 1 cut(s) 709
MnlI CCTC 5 cut(s) 190, 424, 467, 619, 859
MroXI GAANNNNTTC 1 cut(s) 171
MseI TTAA 4 cut(s) 266, 587, 633, 755
MspA1I CMGCKG 1 cut(s) 461
MspI CCGG 1 cut(s) 556
MspR9I CCNGG 1 cut(s) 203
MvaI CCWGG 1 cut(s) 203
MvnI CGCG 1 cut(s) 461
MwoI GCNNNNNNNGC 3 cut(s) 434, 443, 635
NdeII GATC 4 cut(s) 36, 89, 259, 580
NlaIII CATG 2 cut(s) 260, 291
PagI TCATGA 1 cut(s) 256
PcsI WCGNNNNNNNCGW 1 cut(s) 724
PdmI GAANNNNTTC 1 cut(s) 171
PfeI GAWTC 4 cut(s) 217, 284, 380, 782
PkrI GCNGC 5 cut(s) 367, 439, 460, 596, 599
PleI GAGTC 1 cut(s) 709
PpsI GAGTC 1 cut(s) 709
PsiI TTATAA 1 cut(s) 518
Psp1406I AACGTT 2 cut(s) 48, 416
Psp6I CCWGG 1 cut(s) 201
PspGI CCWGG 1 cut(s) 201
PspPI GGNCC 1 cut(s) 199
PstI CTGCAG 1 cut(s) 430
PsuI RGATCY 1 cut(s) 36
RsaI GTAC 2 cut(s) 339, 478
RsaNI GTAC 2 cut(s) 338, 477
SacII CCGCGG 1 cut(s) 462
SalI GTCGAC 1 cut(s) 190
SaqAI TTAA 4 cut(s) 266, 587, 633, 755
SatI GCNGC 5 cut(s) 366, 438, 459, 595, 598
Sau3AI GATC 4 cut(s) 36, 89, 259, 580
Sau96I GGNCC 1 cut(s) 199
SchI GAGTC 1 cut(s) 709
ScrFI CCNGG 1 cut(s) 203
SduI GDGCHC 2 cut(s) 682, 708
SfaNI GCATC 1 cut(s) 214
SfcI CTRYAG 1 cut(s) 426
Sfr303I CCGCGG 1 cut(s) 462
SgrBI CCGCGG 1 cut(s) 462
SinI GGWCC 1 cut(s) 199
Sse9I AATT 5 cut(s) 58, 93, 359, 468, 496
SsiI CCGC 4 cut(s) 459, 461, 598, 629
SspMI CTAG 4 cut(s) 26, 114, 162, 182
StyD4I CCNGG 1 cut(s) 201
TaaI ACNGT 4 cut(s) 395, 514, 817, 835
TaiI ACGT 4 cut(s) 51, 419, 578, 896
TaqI TCGA 5 cut(s) 191, 222, 383, 507, 750
TasI AATT 5 cut(s) 58, 93, 359, 468, 496
TauI GCSGC 2 cut(s) 461, 600
TfiI GAWTC 4 cut(s) 217, 284, 380, 782
Tru1I TTAA 4 cut(s) 266, 587, 633, 755
Tru9I TTAA 4 cut(s) 266, 587, 633, 755
TseI GCWGC 3 cut(s) 365, 437, 594
TspDTI ATGAA 4 cut(s) 276, 575, 660, 792
TspGWI ACGGA 3 cut(s) 209, 339, 355
VpaK11BI GGWCC 1 cut(s) 199
XapI RAATTY 2 cut(s) 359, 496
XbaI TCTAGA 2 cut(s) 113, 161
XmiI GTMKAC 1 cut(s) 191
XmnI GAANNNNTTC 1 cut(s) 171
XspI CTAG 4 cut(s) 26, 114, 162, 182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.