Rroxscaffold_1G00066480

Ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0015404
Physical Location & Seq
Forward (+)
88002969 .. 88003748
780 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00066480.1

Sequence Viewer

Length: 780 bp
ATGGCTGTACATAGTGTTAATCATGATTCTGCAGCTGCACCAGAAGCTATTCTTGAGAACGTGTGGGCCAGCTTTATTGGTGTTGATGGAGGGAATCAAGAAACAAGTAATACACCAGAAATGTCGAAATCATGGGAAGAACTGCCTAGTCTTGATGGGAGAGAAGGATCAATGGAAATTCTTCAAAGGCTACCAAGTCTCGGTAGGTGGATGTCAATGGGAGCTGAGGCCTGGGAAGAGCTTCTCGACACTCACATTATTCCTTCTTGTGATATTGAAAAGTCTTGCAATGAAAAATTAGAGGGAAATGAGGATGCCAAACCGGAAGCGGAAGAGAAGGTTGAGAGAGTAGAGAAAGTGGCAACAAAGCATTACCGCGGTGTTAGGAGACGGCCATGGGGAAAATATGCAGCAGAAATTCGAGACTCATCAAGAAAAGGAGCTAGAGTTTGGCTTGGGACTTTCGAGACTGCTGAGGAGGCAGCTTTGGCATATGACAAAGCTGCTTTAAGAATCAGAGGTACAAAAGCGCATCTTAACTTCCCTTTTGAAACAGTTGCAAAGGCTATGGGACTTGAGAATTTCAGCTTCAACAATATTTGTTTGAATCCTCGTAAGAGAGGAGTGAGAGAGTGGGAAGAAAATGTTGAGGTAATGACTGGAGAACCTGCATTAAAAAGAAACGCAAGCATGGAAAAGATGTTCGGAGATGAGTTTGATGTGTTTGAATTCCAAGACCTGGGAACTGATTACTTGGACAGTTTGCTCTCTTCATTTTGA

Protein Analysis

259

Amino Acids

29.21

Weight (kDa)

4.91

Isoelectric Point (pI)

55.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 124 - 174 2.6e-15 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015404)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G18450
fragaria_vesca FvH4_4g10480
malus_domestica MD05G1306800.v1.1 MD10G1286200.v1.1
prunus_persica Prupe.4G055600_v2.0.a1
pyrus_communis pycom10g23890
rosa_chinensis RchiOBHm_Chr5g0009741
rosa_laevigata RLG00000031690
rosa_multiflora Rmu_sc0000410.1_g000002
rosa_roxburghii Rroxscaffold_1G00066480
rosa_rugosa Rorug04G0445400
rosa_samantha Rh5AG076900 Rh5BG072200 Rh5CG084600 Rh5DG072600
rosa_wichuraiana Rw5G007090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 676
AccB7I CCANNNNNTGG 1 cut(s) 739
AccII CGCG 1 cut(s) 378
AciI CCGC 3 cut(s) 329, 376, 378
AclWI GGATC 1 cut(s) 175
AcoI YGGCCR 1 cut(s) 392
AcsI RAATTY 4 cut(s) 177, 417, 580, 728
AfaI GTAC 2 cut(s) 9, 523
AfiI CCNNNNNNNGG 2 cut(s) 200, 739
AflIII ACRYGT 1 cut(s) 60
AgsI TTSAA 6 cut(s) 185, 278, 551, 592, 607, 728
AjnI CCWGG 2 cut(s) 230, 738
AloI GAACNNNNNNTCC 2 cut(s) 686, 718
AluBI AGCT 9 cut(s) 35, 47, 72, 224, 241, 443, 485, 503, 588
AluI AGCT 9 cut(s) 35, 47, 72, 224, 241, 443, 485, 503, 588
Alw26I GTCTC 4 cut(s) 203, 382, 417, 461
AlwI GGATC 1 cut(s) 175
AoxI GGCC 3 cut(s) 66, 228, 392
ApeKI GCWGC 5 cut(s) 32, 35, 410, 482, 503
ApoI RAATTY 4 cut(s) 177, 417, 580, 728
Asp700I GAANNNNTTC 3 cut(s) 48, 180, 240
AspLEI GCGC 1 cut(s) 532
AspS9I GGNCC 1 cut(s) 66
BbvCI CCTCAGC 2 cut(s) 225, 474
BbvI GCAGC 5 cut(s) 22, 44, 422, 490, 494
BccI CCATC 2 cut(s) 80, 149
BceAI ACGGC 1 cut(s) 407
BciT130I CCWGG 2 cut(s) 232, 740
BcoDI GTCTC 4 cut(s) 203, 382, 417, 461
BfaI CTAG 2 cut(s) 147, 444
BfmI CTRYAG 1 cut(s) 30
BfuAI ACCTGC 1 cut(s) 676
BisI GCNGC 5 cut(s) 33, 36, 411, 483, 504
BlsI GCNGC 5 cut(s) 34, 37, 412, 484, 505
Bme1390I CCNGG 2 cut(s) 232, 740
BmgT120I GGNCC 1 cut(s) 66
BmrFI CCNGG 2 cut(s) 232, 740
BmsI GCATC 2 cut(s) 304, 541
BpmI CTGGAG 1 cut(s) 681
Bpu10I CCTNAGC 2 cut(s) 225, 474
BpuEI CTTGAG 2 cut(s) 74, 596
BsaJI CCNNGG 4 cut(s) 231, 376, 395, 739
BsaWI WCCGGW 1 cut(s) 322
BsaXI ACNNNNNCTCC 2 cut(s) 432, 462
Bsc4I CCNNNNNNNGG 2 cut(s) 200, 739
Bse1I ACTGG 1 cut(s) 664
Bse3DI GCAATG 1 cut(s) 295
BseBI CCWGG 2 cut(s) 232, 740
BseDI CCNNGG 4 cut(s) 231, 376, 395, 739
BseGI GGATG 2 cut(s) 216, 319
BseLI CCNNNNNNNGG 2 cut(s) 200, 739
BseMI GCAATG 1 cut(s) 295
BseMII CTCAG 2 cut(s) 216, 465
BseNI ACTGG 1 cut(s) 664
BseRI GAGGAG 2 cut(s) 491, 636
BseXI GCAGC 5 cut(s) 22, 44, 422, 490, 494
BsgI GTGCAG 1 cut(s) 21
Bsh1236I CGCG 1 cut(s) 378
BshFI GGCC 3 cut(s) 68, 230, 394
BsiSI CCGG 1 cut(s) 323
BslFI GGGAC 2 cut(s) 472, 585
BslI CCNNNNNNNGG 2 cut(s) 200, 739
BsmAI GTCTC 4 cut(s) 203, 382, 417, 461
BsmBI CGTCTC 1 cut(s) 382
BsmFI GGGAC 2 cut(s) 472, 585
BsnI GGCC 3 cut(s) 68, 230, 394
Bsp1407I TGTACA 1 cut(s) 7
Bsp143I GATC 1 cut(s) 167
Bsp19I CCATGG 1 cut(s) 395
BspACI CCGC 3 cut(s) 329, 376, 378
BspANI GGCC 3 cut(s) 68, 230, 394
BspCNI CTCAG 2 cut(s) 217, 466
BspFNI CGCG 1 cut(s) 378
BspHI TCATGA 1 cut(s) 22
BspMAI CTGCAG 1 cut(s) 34
BspMI ACCTGC 1 cut(s) 676
BspPI GGATC 1 cut(s) 175
BspQI GCTCTTC 1 cut(s) 231
BsrDI GCAATG 1 cut(s) 295
BsrGI TGTACA 1 cut(s) 7
BsrI ACTGG 1 cut(s) 664
BssECI CCNNGG 4 cut(s) 231, 376, 395, 739
BssMI GATC 1 cut(s) 167
BssT1I CCWWGG 1 cut(s) 395
Bst2UI CCWGG 2 cut(s) 232, 740
Bst4CI ACNGT 2 cut(s) 556, 761
Bst6I CTCTTC 3 cut(s) 231, 327, 775
BstAUI TGTACA 1 cut(s) 7
BstC8I GCNNGC 2 cut(s) 70, 688
BstDEI CTNAG 2 cut(s) 225, 474
BstDSI CCRYGG 2 cut(s) 376, 395
BstF5I GGATG 2 cut(s) 216, 319
BstFNI CGCG 1 cut(s) 378
BstHHI GCGC 1 cut(s) 532
BstKTI GATC 1 cut(s) 170
BstMAI GTCTC 4 cut(s) 203, 382, 417, 461
BstMBI GATC 1 cut(s) 167
BstMWI GCNNNNNNNGC 3 cut(s) 44, 479, 488
BstNI CCWGG 2 cut(s) 232, 740
BstSCI CCNGG 2 cut(s) 230, 738
BstSFI CTRYAG 1 cut(s) 30
BstUI CGCG 1 cut(s) 378
BstV1I GCAGC 5 cut(s) 22, 44, 422, 490, 494
BsuRI GGCC 3 cut(s) 68, 230, 394
BtgI CCRYGG 2 cut(s) 376, 395
BtsCI GGATG 2 cut(s) 216, 319
BveI ACCTGC 1 cut(s) 676
Cac8I GCNNGC 2 cut(s) 70, 688
CciI TCATGA 1 cut(s) 22
CfoI GCGC 1 cut(s) 532
Cfr13I GGNCC 1 cut(s) 66
Cfr42I CCGCGG 1 cut(s) 379
Csp6I GTAC 2 cut(s) 8, 522
CviAII CATG 4 cut(s) 23, 132, 396, 691
CviQI GTAC 2 cut(s) 8, 522
DdeI CTNAG 2 cut(s) 225, 474
DpnI GATC 1 cut(s) 169
DpnII GATC 1 cut(s) 167
EaeI YGGCCR 1 cut(s) 392
Eam1104I CTCTTC 3 cut(s) 231, 327, 775
EarI CTCTTC 3 cut(s) 231, 327, 775
Eco130I CCWWGG 1 cut(s) 395
Eco147I AGGCCT 1 cut(s) 230
EcoRI GAATTC 1 cut(s) 728
EcoRII CCWGG 2 cut(s) 230, 738
EcoT14I CCWWGG 1 cut(s) 395
ErhI CCWWGG 1 cut(s) 395
Esp3I CGTCTC 1 cut(s) 382
FaeI CATG 4 cut(s) 26, 135, 399, 694
FaiI YATR 9 cut(s) 12, 24, 133, 397, 408, 493, 495, 569, 692
FalI AAGNNNNNCTT 4 cut(s) 36, 68, 519, 551
FaqI GGGAC 2 cut(s) 472, 585
FatI CATG 4 cut(s) 22, 131, 395, 690
FauNDI CATATG 1 cut(s) 493
Fnu4HI GCNGC 5 cut(s) 33, 36, 411, 483, 504
FokI GGATG 2 cut(s) 223, 326
Fsp4HI GCNGC 5 cut(s) 33, 36, 411, 483, 504
FspBI CTAG 2 cut(s) 147, 444
GlaI GCGC 1 cut(s) 531
GluI GCNGC 5 cut(s) 33, 36, 411, 483, 504
GsuI CTGGAG 1 cut(s) 681
HaeIII GGCC 3 cut(s) 68, 230, 394
HapII CCGG 1 cut(s) 323
HhaI GCGC 1 cut(s) 532
Hin1II CATG 4 cut(s) 26, 135, 399, 694
Hin6I GCGC 1 cut(s) 530
HinP1I GCGC 1 cut(s) 530
HinfI GANTC 5 cut(s) 26, 94, 425, 513, 607
HpaII CCGG 1 cut(s) 323
Hpy188I TCNGA 2 cut(s) 518, 707
Hpy188III TCNNGA 8 cut(s) 23, 53, 98, 152, 245, 422, 432, 466
HpyAV CCTTC 3 cut(s) 158, 273, 331
HpyCH4III ACNGT 2 cut(s) 556, 761
HpyCH4IV ACGT 1 cut(s) 60
HpyCH4V TGCA 6 cut(s) 32, 38, 288, 410, 560, 671
HpyF10VI GCNNNNNNNGC 3 cut(s) 44, 479, 488
HpyF3I CTNAG 2 cut(s) 225, 474
HpySE526I ACGT 1 cut(s) 60
Hsp92II CATG 4 cut(s) 26, 135, 399, 694
HspAI GCGC 1 cut(s) 530
KspI CCGCGG 1 cut(s) 379
Kzo9I GATC 1 cut(s) 167
LguI GCTCTTC 1 cut(s) 231
LmnI GCTCC 2 cut(s) 221, 440
Lsp1109I GCAGC 5 cut(s) 22, 44, 422, 490, 494
LweI GCATC 2 cut(s) 304, 541
MaeI CTAG 2 cut(s) 147, 444
MaeII ACGT 1 cut(s) 60
MalI GATC 1 cut(s) 169
MboI GATC 1 cut(s) 167
MboII GAAGA 6 cut(s) 149, 173, 248, 344, 650, 762
MluCI AATT 5 cut(s) 177, 296, 417, 580, 728
MlyI GAGTC 1 cut(s) 419
MroXI GAANNNNTTC 3 cut(s) 48, 180, 240
MseI TTAA 4 cut(s) 18, 509, 537, 674
MspA1I CMGCKG 2 cut(s) 35, 378
MspI CCGG 1 cut(s) 323
MspR9I CCNGG 2 cut(s) 232, 740
MvaI CCWGG 2 cut(s) 232, 740
MvnI CGCG 1 cut(s) 378
MwoI GCNNNNNNNGC 3 cut(s) 44, 479, 488
NcoI CCATGG 1 cut(s) 395
NdeI CATATG 1 cut(s) 493
NdeII GATC 1 cut(s) 167
NlaIII CATG 4 cut(s) 26, 135, 399, 694
PagI TCATGA 1 cut(s) 22
PceI AGGCCT 1 cut(s) 230
PciSI GCTCTTC 1 cut(s) 231
PdmI GAANNNNTTC 3 cut(s) 48, 180, 240
PfeI GAWTC 4 cut(s) 26, 94, 513, 607
PflMI CCANNNNNTGG 1 cut(s) 739
PkrI GCNGC 5 cut(s) 34, 37, 412, 484, 505
PleI GAGTC 1 cut(s) 419
PpsI GAGTC 1 cut(s) 419
Psp6I CCWGG 2 cut(s) 230, 738
PspGI CCWGG 2 cut(s) 230, 738
PspPI GGNCC 1 cut(s) 66
PstI CTGCAG 1 cut(s) 34
PvuII CAGCTG 1 cut(s) 35
RsaI GTAC 2 cut(s) 9, 523
RsaNI GTAC 2 cut(s) 8, 522
SacII CCGCGG 1 cut(s) 379
SapI GCTCTTC 1 cut(s) 231
SaqAI TTAA 4 cut(s) 18, 509, 537, 674
SatI GCNGC 5 cut(s) 33, 36, 411, 483, 504
Sau3AI GATC 1 cut(s) 167
Sau96I GGNCC 1 cut(s) 66
SchI GAGTC 1 cut(s) 419
ScrFI CCNGG 2 cut(s) 232, 740
SfaNI GCATC 2 cut(s) 304, 541
SfcI CTRYAG 1 cut(s) 30
Sfr303I CCGCGG 1 cut(s) 379
SgrBI CCGCGG 1 cut(s) 379
SmlI CTYRAG 2 cut(s) 53, 575
SmoI CTYRAG 2 cut(s) 53, 575
Sse9I AATT 5 cut(s) 177, 296, 417, 580, 728
SseBI AGGCCT 1 cut(s) 230
SsiI CCGC 3 cut(s) 329, 376, 378
SspI AATATT 1 cut(s) 598
SspMI CTAG 2 cut(s) 147, 444
StuI AGGCCT 1 cut(s) 230
StyD4I CCNGG 2 cut(s) 230, 738
StyI CCWWGG 1 cut(s) 395
TaaI ACNGT 2 cut(s) 556, 761
TaiI ACGT 1 cut(s) 63
TaqI TCGA 4 cut(s) 125, 246, 421, 465
TasI AATT 5 cut(s) 177, 296, 417, 580, 728
TatI WGTACW 1 cut(s) 7
TfiI GAWTC 4 cut(s) 26, 94, 513, 607
Tru1I TTAA 4 cut(s) 18, 509, 537, 674
Tru9I TTAA 4 cut(s) 18, 509, 537, 674
TseI GCWGC 5 cut(s) 32, 35, 410, 482, 503
TspDTI ATGAA 2 cut(s) 306, 762
Van91I CCANNNNNTGG 1 cut(s) 739
XapI RAATTY 4 cut(s) 177, 417, 580, 728
XmnI GAANNNNTTC 3 cut(s) 48, 180, 240
XspI CTAG 2 cut(s) 147, 444
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.