pycom10g23890

Ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0015404
Physical Location & Seq
Forward (+)
25856575 .. 25857348
774 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g23890.1

Sequence Viewer

Length: 774 bp
ATGACTGTACATAGTGATATTCATGCAGAAAAGCCAGCATCTGAAGCTATAGTACTAGAGAACGTGTGGGCGAGCTTTATCGGTGTGGATGGACCGAAAAACGCAACAGAATTGTCTCAATCCTGGGAAGATATGCCTCCTCTTGATGGGAGAGAAGGTTCAATGGAAGTTCTACAAAGGCTACCAAGTCTAGGTAGGTGGATATCAATGGGAACTGAGGCCTGGGAGGAGCTTCTGGACAGTGTTGTTGGTCCTGCACAAAATACGGAGCAGTCTTGCAATGACAAGTTAGAAGGCAATGGAGCTACAAATTCGGAATGTGAAGCAAAGGTCGAGAGAGCAAAGAAAGTGGAAATTGTGAAGCACTACCGCGGAGTTAGGAGGCGGCCGTGGGGCAAGTATGCAGCAGAGATTAGGGACTCAACAAGAAAAGGGGCTAGGGTTTGGCTTGGGACTTTTAAGACTGCTGAGGAGGCCGCTTTGGCTTATGACAAGGCTGCTTTGAGAATTAGAGGCTCAAAGGCACATCTTAACTTCCCCCTTGAAACAGTTGCAAAGGCTATGGGATTGGATTGCTTCAGCCTTTCTTCATCCTCAATCGGTTCGAATCCTCGGAAGAGAGGGTTGAGCGATTGGGAAGAAAATGTTGAGGAAAGAAAACCTGCAGTGAAGAGGAAGGCAAGCATGCATAAGATGTGTGATCATGAATTTGATGTATTTGAGTTCCAAGACCTGGGAAGTGACTACTTGGACAGTTTGCTGTCTTCTTTTTGA

Protein Analysis

258

Amino Acids

28.68

Weight (kDa)

5.64

Isoelectric Point (pI)

51.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 122 - 172 3.2e-15 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015404)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G18450
fragaria_vesca FvH4_4g10480
malus_domestica MD05G1306800.v1.1 MD10G1286200.v1.1
prunus_persica Prupe.4G055600_v2.0.a1
pyrus_communis pycom10g23890
rosa_chinensis RchiOBHm_Chr5g0009741
rosa_laevigata RLG00000031690
rosa_multiflora Rmu_sc0000410.1_g000002
rosa_roxburghii Rroxscaffold_1G00066480
rosa_rugosa Rorug04G0445400
rosa_samantha Rh5AG076900 Rh5BG072200 Rh5CG084600 Rh5DG072600
rosa_wichuraiana Rw5G007090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 670
AccB7I CCANNNNNTGG 1 cut(s) 733
AccII CGCG 1 cut(s) 372
AciI CCGC 4 cut(s) 370, 372, 385, 477
AcoI YGGCCR 1 cut(s) 386
AcsI RAATTY 2 cut(s) 310, 707
AcuI CTGAAG 2 cut(s) 63, 562
AfaI GTAC 2 cut(s) 9, 54
AfiI CCNNNNNNNGG 3 cut(s) 146, 191, 733
AflIII ACRYGT 1 cut(s) 63
AgsI TTSAA 2 cut(s) 162, 545
AjnI CCWGG 3 cut(s) 122, 221, 732
AloI GAACNNNNNNTCC 2 cut(s) 142, 174
AluBI AGCT 4 cut(s) 47, 75, 232, 305
AluI AGCT 4 cut(s) 47, 75, 232, 305
Alw26I GTCTC 1 cut(s) 120
AlwNI CAGNNNCTG 1 cut(s) 41
AoxI GGCC 3 cut(s) 219, 386, 474
ApeKI GCWGC 2 cut(s) 404, 497
ApoI RAATTY 2 cut(s) 310, 707
AspS9I GGNCC 2 cut(s) 92, 251
AsuII TTCGAA 1 cut(s) 605
AvaII GGWCC 2 cut(s) 92, 251
BarI GAAGNNNNNNTAC 2 cut(s) 36, 68
BbsI GAAGAC 1 cut(s) 756
BbvCI CCTCAGC 1 cut(s) 468
BbvI GCAGC 2 cut(s) 416, 484
BccI CCATC 2 cut(s) 83, 140
BceAI ACGGC 1 cut(s) 373
BciT130I CCWGG 3 cut(s) 124, 223, 734
BclI TGATCA 1 cut(s) 700
BcoDI GTCTC 1 cut(s) 120
BfaI CTAG 3 cut(s) 56, 191, 438
BfmI CTRYAG 2 cut(s) 48, 663
BfuAI ACCTGC 1 cut(s) 670
BglI GCCNNNNNGGC 1 cut(s) 482
BisI GCNGC 4 cut(s) 386, 405, 477, 498
BlsI GCNGC 4 cut(s) 387, 406, 478, 499
BmcAI AGTACT 1 cut(s) 54
Bme1390I CCNGG 3 cut(s) 124, 223, 734
Bme18I GGWCC 2 cut(s) 92, 251
BmgT120I GGNCC 2 cut(s) 92, 251
BmrFI CCNGG 3 cut(s) 124, 223, 734
BmsI GCATC 1 cut(s) 47
BpiI GAAGAC 1 cut(s) 756
Bpu10I CCTNAGC 1 cut(s) 468
Bpu14I TTCGAA 1 cut(s) 605
BsaJI CCNNGG 6 cut(s) 123, 222, 370, 389, 611, 733
BsaXI ACNNNNNCTCC 2 cut(s) 142, 172
Bsc4I CCNNNNNNNGG 3 cut(s) 146, 191, 733
Bse3DI GCAATG 2 cut(s) 286, 304
BseBI CCWGG 3 cut(s) 124, 223, 734
BseDI CCNNGG 6 cut(s) 123, 222, 370, 389, 611, 733
BseGI GGATG 2 cut(s) 94, 590
BseLI CCNNNNNNNGG 3 cut(s) 146, 191, 733
BseMI GCAATG 2 cut(s) 286, 304
BseMII CTCAG 2 cut(s) 207, 459
BseRI GAGGAG 3 cut(s) 129, 242, 485
BseX3I CGGCCG 1 cut(s) 386
BseXI GCAGC 2 cut(s) 416, 484
BsgI GTGCAG 1 cut(s) 240
Bsh1236I CGCG 1 cut(s) 372
Bsh1285I CGRYCG 1 cut(s) 389
BshFI GGCC 3 cut(s) 221, 388, 476
BsiEI CGRYCG 1 cut(s) 389
BslFI GGGAC 2 cut(s) 431, 466
BslI CCNNNNNNNGG 3 cut(s) 146, 191, 733
BsmAI GTCTC 1 cut(s) 120
BsmFI GGGAC 2 cut(s) 431, 466
BsnI GGCC 3 cut(s) 221, 388, 476
Bsp119I TTCGAA 1 cut(s) 605
Bsp1407I TGTACA 1 cut(s) 7
Bsp143I GATC 1 cut(s) 700
BspACI CCGC 4 cut(s) 370, 372, 385, 477
BspANI GGCC 3 cut(s) 221, 388, 476
BspCNI CTCAG 2 cut(s) 208, 460
BspFNI CGCG 1 cut(s) 372
BspHI TCATGA 1 cut(s) 703
BspMAI CTGCAG 1 cut(s) 667
BspMI ACCTGC 1 cut(s) 670
BspT104I TTCGAA 1 cut(s) 605
BsrDI GCAATG 2 cut(s) 286, 304
BsrGI TGTACA 1 cut(s) 7
BssECI CCNNGG 6 cut(s) 123, 222, 370, 389, 611, 733
BssMI GATC 1 cut(s) 700
Bst2UI CCWGG 3 cut(s) 124, 223, 734
Bst4CI ACNGT 4 cut(s) 7, 242, 550, 755
Bst6I CTCTTC 2 cut(s) 611, 665
BstAUI TGTACA 1 cut(s) 7
BstBI TTCGAA 1 cut(s) 605
BstC8I GCNNGC 4 cut(s) 36, 73, 682, 686
BstDEI CTNAG 2 cut(s) 216, 468
BstDSI CCRYGG 2 cut(s) 370, 389
BstF5I GGATG 2 cut(s) 94, 590
BstFNI CGCG 1 cut(s) 372
BstKTI GATC 1 cut(s) 703
BstMAI GTCTC 1 cut(s) 120
BstMBI GATC 1 cut(s) 700
BstMCI CGRYCG 1 cut(s) 389
BstMWI GCNNNNNNNGC 3 cut(s) 44, 473, 482
BstNI CCWGG 3 cut(s) 124, 223, 734
BstNSI RCATGY 1 cut(s) 688
BstSCI CCNGG 3 cut(s) 122, 221, 732
BstSFI CTRYAG 2 cut(s) 48, 663
BstUI CGCG 1 cut(s) 372
BstV1I GCAGC 2 cut(s) 416, 484
BstV2I GAAGAC 1 cut(s) 756
BstZI CGGCCG 1 cut(s) 386
BsuRI GGCC 3 cut(s) 221, 388, 476
BtgI CCRYGG 2 cut(s) 370, 389
BtsCI GGATG 2 cut(s) 94, 590
BtsI GCAGTG 1 cut(s) 672
BtsIMutI CAGTG 2 cut(s) 247, 672
BveI ACCTGC 1 cut(s) 670
Cac8I GCNNGC 4 cut(s) 36, 73, 682, 686
CaiI CAGNNNCTG 1 cut(s) 41
CciI TCATGA 1 cut(s) 703
Cfr13I GGNCC 2 cut(s) 92, 251
Cfr42I CCGCGG 1 cut(s) 373
Csp6I GTAC 2 cut(s) 8, 53
CspCI CAANNNNNGTGG 2 cut(s) 330, 365
CviAII CATG 3 cut(s) 23, 685, 704
CviQI GTAC 2 cut(s) 8, 53
DdeI CTNAG 2 cut(s) 216, 468
DpnI GATC 1 cut(s) 702
DpnII GATC 1 cut(s) 700
EaeI YGGCCR 1 cut(s) 386
EagI CGGCCG 1 cut(s) 386
Eam1104I CTCTTC 2 cut(s) 611, 665
EarI CTCTTC 2 cut(s) 611, 665
EclXI CGGCCG 1 cut(s) 386
Eco147I AGGCCT 1 cut(s) 221
Eco32I GATATC 1 cut(s) 204
Eco47I GGWCC 2 cut(s) 92, 251
Eco52I CGGCCG 1 cut(s) 386
Eco57I CTGAAG 2 cut(s) 63, 562
EcoRII CCWGG 3 cut(s) 122, 221, 732
EcoRV GATATC 1 cut(s) 204
EcoT22I ATGCAT 1 cut(s) 690
FaeI CATG 3 cut(s) 26, 688, 707
FaqI GGGAC 2 cut(s) 431, 466
FatI CATG 3 cut(s) 22, 684, 703
FbaI TGATCA 1 cut(s) 700
Fnu4HI GCNGC 4 cut(s) 386, 405, 477, 498
FokI GGATG 2 cut(s) 101, 577
Fsp4HI GCNGC 4 cut(s) 386, 405, 477, 498
FspBI CTAG 3 cut(s) 56, 191, 438
GluI GCNGC 4 cut(s) 386, 405, 477, 498
HaeIII GGCC 3 cut(s) 221, 388, 476
Hin1II CATG 3 cut(s) 26, 688, 707
HinfI GANTC 2 cut(s) 419, 607
Hpy188I TCNGA 3 cut(s) 43, 316, 615
Hpy188III TCNNGA 4 cut(s) 143, 236, 334, 704
HpyAV CCTTC 3 cut(s) 149, 287, 670
HpyCH4III ACNGT 4 cut(s) 7, 242, 550, 755
HpyCH4IV ACGT 1 cut(s) 63
HpyCH4V TGCA 7 cut(s) 26, 257, 279, 404, 554, 665, 688
HpyF10VI GCNNNNNNNGC 3 cut(s) 44, 473, 482
HpyF3I CTNAG 2 cut(s) 216, 468
HpySE526I ACGT 1 cut(s) 63
Hsp92II CATG 3 cut(s) 26, 688, 707
Ksp22I TGATCA 1 cut(s) 700
KspI CCGCGG 1 cut(s) 373
Kzo9I GATC 1 cut(s) 700
LmnI GCTCC 3 cut(s) 229, 268, 302
Lsp1109I GCAGC 2 cut(s) 416, 484
LweI GCATC 1 cut(s) 47
MaeI CTAG 3 cut(s) 56, 191, 438
MaeII ACGT 1 cut(s) 63
MaeIII GTNAC 1 cut(s) 740
MalI GATC 1 cut(s) 702
MboI GATC 1 cut(s) 700
MboII GAAGA 6 cut(s) 140, 579, 628, 650, 682, 756
MluCI AATT 5 cut(s) 110, 310, 354, 507, 707
MlyI GAGTC 1 cut(s) 413
Mph1103I ATGCAT 1 cut(s) 690
MseI TTAA 2 cut(s) 459, 531
MspA1I CMGCKG 1 cut(s) 372
MspR9I CCNGG 3 cut(s) 124, 223, 734
MvaI CCWGG 3 cut(s) 124, 223, 734
MvnI CGCG 1 cut(s) 372
MwoI GCNNNNNNNGC 3 cut(s) 44, 473, 482
NdeII GATC 1 cut(s) 700
NlaIII CATG 3 cut(s) 26, 688, 707
NmuCI GTSAC 1 cut(s) 740
NsiI ATGCAT 1 cut(s) 690
NspI RCATGY 1 cut(s) 688
NspV TTCGAA 1 cut(s) 605
PaeI GCATGC 1 cut(s) 688
PagI TCATGA 1 cut(s) 703
PceI AGGCCT 1 cut(s) 221
PfeI GAWTC 1 cut(s) 607
PflMI CCANNNNNTGG 1 cut(s) 733
PkrI GCNGC 4 cut(s) 387, 406, 478, 499
PleI GAGTC 1 cut(s) 413
PpsI GAGTC 1 cut(s) 413
Psp6I CCWGG 3 cut(s) 122, 221, 732
PspGI CCWGG 3 cut(s) 122, 221, 732
PspPI GGNCC 2 cut(s) 92, 251
PstI CTGCAG 1 cut(s) 667
PstNI CAGNNNCTG 1 cut(s) 41
RsaI GTAC 2 cut(s) 9, 54
RsaNI GTAC 2 cut(s) 8, 53
SacII CCGCGG 1 cut(s) 373
SaqAI TTAA 2 cut(s) 459, 531
SatI GCNGC 4 cut(s) 386, 405, 477, 498
Sau3AI GATC 1 cut(s) 700
Sau96I GGNCC 2 cut(s) 92, 251
ScaI AGTACT 1 cut(s) 54
SchI GAGTC 1 cut(s) 413
ScrFI CCNGG 3 cut(s) 124, 223, 734
SfaNI GCATC 1 cut(s) 47
SfcI CTRYAG 2 cut(s) 48, 663
Sfr303I CCGCGG 1 cut(s) 373
SfuI TTCGAA 1 cut(s) 605
SgrBI CCGCGG 1 cut(s) 373
SinI GGWCC 2 cut(s) 92, 251
SphI GCATGC 1 cut(s) 688
Sse9I AATT 5 cut(s) 110, 310, 354, 507, 707
SseBI AGGCCT 1 cut(s) 221
SsiI CCGC 4 cut(s) 370, 372, 385, 477
SspMI CTAG 3 cut(s) 56, 191, 438
StuI AGGCCT 1 cut(s) 221
StyD4I CCNGG 3 cut(s) 122, 221, 732
TaaI ACNGT 4 cut(s) 7, 242, 550, 755
TaiI ACGT 1 cut(s) 66
TaqI TCGA 2 cut(s) 333, 605
TaqII GACCGA 1 cut(s) 109
TasI AATT 5 cut(s) 110, 310, 354, 507, 707
TatI WGTACW 2 cut(s) 7, 52
TauI GCSGC 2 cut(s) 388, 479
TfiI GAWTC 1 cut(s) 607
Tru1I TTAA 2 cut(s) 459, 531
Tru9I TTAA 2 cut(s) 459, 531
TscAI CASTG 2 cut(s) 247, 672
TseFI GTSAC 1 cut(s) 740
TseI GCWGC 2 cut(s) 404, 497
Tsp45I GTSAC 1 cut(s) 740
TspDTI ATGAA 3 cut(s) 11, 579, 720
TspGWI ACGGA 1 cut(s) 281
TspRI CASTG 2 cut(s) 247, 672
Van91I CCANNNNNTGG 1 cut(s) 733
VpaK11BI GGWCC 2 cut(s) 92, 251
XapI RAATTY 2 cut(s) 310, 707
XceI RCATGY 1 cut(s) 688
XspI CTAG 3 cut(s) 56, 191, 438
ZrmI AGTACT 1 cut(s) 54
Zsp2I ATGCAT 1 cut(s) 690
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.