Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0007972

Physical Location & Seq

Genomic Coordinates Reverse (-)

11447429 .. 11450571

Gene Structure 3143 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT4G21520.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1278 bp

ATGGGTGAAGAAGAAGCAGTAGCAGAAGAAAATGGTGGACTTAAGGTAGAATCAGGAGAACAAAAATCATCCTGGCCTACTATGAGATTTGATGTCTCTCCTTATAGAACGCACCATTTCTCTAAGCAGTTCAGGACAGCTCGAAACCCTAATAATTTCCTCAAGGGTCTTAAGTGGTCACCTGATGGTTCATGTTTCCTTGCAAGCTCTGAAGATAATACACTTAGTCTGTTTCATCTGCCACAAGATGGAGGGGATTCTAATGGCTATGGAGTGCCAGTCCCTGAAGAAGATTCGTATGGTGCGAGTCTTCTAGTGAATGAGGGTGAATCTGTCTATGATTTCTGTTGGTATCCATACATGTCAGTTTCAGACCCTTTAACATGCGTCTTCGCTACCTCCACTAGAGACCATCCGATACATCTTTGGGATAGTACTTCTGGTGAGCTTCGATGCACATACCGGGCCTATGATGCTATGGATGAAATAACTGCTGCATTTTCAGTTGGATTCAATCCTGATGGAACCAAGATCTTTGCTGGTTACAACAGCTCCATCAGAGTGTTTGATCTTCATCGTCCTGGAAGAGATTTTAGGCAGTATTCAACTCTACAGAAAAACAAAGAGGGCCAAGCAGGTATATTATCTACTCTTGCTTTCTCCCCAACTAATTCTGGAATGTTGGCTGTGGGGTCTTACGGGCAAACTACTGGGATTTATAGAGAAGACAACATGGAGCTATTGTATGTTTTACATGGTCAAGAAGGCGGTGTTACACATGTCCAGTTTTCAAAGGATGGGAACTATTTATATACAGGAGGTCGCAAGGATCCTTACATTCTTTGCTGGGATATTCGGAAATCTGTTGAAATAGTCTATAAATTGTACAGAGCAACTGAGAACACAAACCAGCGTGTATTCTTTGATATTGAGCCATGTGGTCGACATTTAGGTACCGGTGGTCAGGATGGGCTTGTTCACATGTACGATTTACAAACCGGAAACTGGGTTTCTGGATATCAAGCTGCTTCAGATACCGTCAATGCTTTCTCTTTCCACCCTTACCTTCCAATGGCTGCTACATCCTCTGGTCACAGACGTTTCGCAATTCCAGATGATGACGATGGTGAAGATAAGAACGAGCTTCAATTGAAAGCTGATGAGAACTGTGTATCTCTCTGGAGTTTCTACGTAGAGGACAATACTTATGATGAAAACAATGGCGTTGCAAGCGAGTCTCATCTCCAAAATGTCACCGAAGAAATAGTTTTAACATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005697 GO:0006996 GO:0008150 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010556 GO:0010557 GO:0010604 GO:0015030 GO:0016043 GO:0016604 GO:0019219 GO:0019222 GO:0022607 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031974 GO:0031981 GO:0032200 GO:0032202 GO:0032203 GO:0032991 GO:0043085 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044085 GO:0044093 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045935 GO:0048518 GO:0048522 GO:0050789 GO:0050790 GO:0050794 GO:0051052 GO:0051054 GO:0051171 GO:0051173 GO:0051276 GO:0051338 GO:0051347 GO:0051972 GO:0051973 GO:0060255 GO:0065007 GO:0065009 GO:0070013 GO:0071840 GO:0080090 GO:0097159 GO:1901363 GO:1990904 GO:2000112 GO:2000278 GO:2000573

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

425

Amino Acids

47.54

Weight (kDa)

4.76

Isoelectric Point (pI)

37.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_THOC3	PF25174	55 - 289	2.2e-16	THOC3 beta-propeller domain
Beta-prop_EML_2	PF23414	126 - 290	1.6e-12	Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz	PF24807	139 - 289	7.8e-07	CDC20/Fizzy WD40 domain
WD40_WDHD1_1st	PF24817	165 - 291	2.9e-08	WDHD1 first WD40 domain
WD40_Prp19	PF24814	197 - 310	6.1e-07	Prp19 WD40 domain
Beta-prop_WDR3_1st	PF25173	209 - 363	3e-13	WDR3 first beta-propeller domain
Beta-prop_WDR5	PF25175	211 - 397	5.7e-15	WDR5 beta-propeller domain
WD40_CDC20-Fz	PF24807	211 - 358	1e-09	CDC20/Fizzy WD40 domain
WDR55	PF24796	216 - 363	5.1e-08	WDR55
Beta-prop_TEP1_2nd	PF25047	224 - 288	1e-07	TEP-1 second beta-propeller

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0007972)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G21520 AT4G21520
fragaria_vesca	FvH4_3g04030 FvH4_3g04030 FvH4_3g04030 FvH4_3g04030 FvH4_3g04030 FvH4_3g04030
malus_domestica	MD05G1325300.v1.1 MD10G1303200.v1.1
prunus_persica	Prupe.4G036600_v2.0.a1
pyrus_communis	pycom05g29930 pycom10g25570
rosa_chinensis	RchiOBHm_Chr5g0006371
rosa_laevigata	RLG00000031362
rosa_multiflora	Rmu_sc0037704.1_g000001
rosa_roxburghii	Rroxscaffold_1G00069560
rosa_rugosa	Rorug04G0421200 Rorug04G0421200
rosa_samantha	Rh5AG049200 Rh5BG048500 Rh5CG056700 Rh5DG047800
rosa_wichuraiana	Rw5G004900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	626
Acc65I	GGTACC	1 cut(s)	953
AccB1I	GGYRCC	1 cut(s)	953
AccB7I	CCANNNNNTGG	1 cut(s)	248
AccI	GTMKAC	1 cut(s)	943
AciI	CCGC	1 cut(s)	768
AclWI	GGATC	2 cut(s)	824, 837
AcuI	CTGAAG	3 cut(s)	231, 306, 1014
AfaI	GTAC	4 cut(s)	436, 887, 955, 986
AfiI	CCNNNNNNNGG	3 cut(s)	248, 1005, 1072
AflII	CTTAAG	2 cut(s)	41, 170
AflIII	ACRYGT	3 cut(s)	360, 778, 981
AgeI	ACCGGT	1 cut(s)	956
AgsI	TTSAA	6 cut(s)	514, 606, 792, 869, 1148, 1153
AjnI	CCWGG	2 cut(s)	71, 580
AluBI	AGCT	8 cut(s)	140, 207, 448, 552, 739, 1025, 1144, 1157
AluI	AGCT	8 cut(s)	140, 207, 448, 552, 739, 1025, 1144, 1157
Alw26I	GTCTC	3 cut(s)	100, 402, 1242
AlwI	GGATC	2 cut(s)	824, 837
AlwNI	CAGNNNCTG	1 cut(s)	284
AoxI	GGCC	3 cut(s)	74, 465, 628
ApeKI	GCWGC	3 cut(s)	494, 1025, 1076
AsiGI	ACCGGT	1 cut(s)	956
Asp718I	GGTACC	1 cut(s)	953
AspS9I	GGNCC	2 cut(s)	465, 628
AsuC2I	CCSGG	1 cut(s)	464
AsuHPI	GGTGA	6 cut(s)	17, 171, 338, 455, 1139, 1246
BamHI	GGATCC	1 cut(s)	829
BanI	GGYRCC	1 cut(s)	953
BbsI	GAAGAC	3 cut(s)	302, 382, 732
BbvI	GCAGC	3 cut(s)	481, 1012, 1063
BccI	CCATC	8 cut(s)	179, 242, 420, 515, 563, 791, 962, 1118
BciT130I	CCWGG	2 cut(s)	73, 582
BciVI	GTATCC	1 cut(s)	363
BcnI	CCSGG	1 cut(s)	464
BcoDI	GTCTC	3 cut(s)	100, 402, 1242
BfaI	CTAG	2 cut(s)	314, 405
BfmI	CTRYAG	1 cut(s)	611
BfrI	CTTAAG	2 cut(s)	41, 170
BfuAI	ACCTGC	1 cut(s)	626
BfuI	GTATCC	1 cut(s)	363
BglII	AGATCT	1 cut(s)	531
BisI	GCNGC	3 cut(s)	495, 1026, 1077
BlsI	GCNGC	3 cut(s)	496, 1027, 1078
BmcAI	AGTACT	1 cut(s)	436
Bme1390I	CCNGG	3 cut(s)	73, 464, 582
BmgT120I	GGNCC	2 cut(s)	465, 628
BmiI	GGNNCC	3 cut(s)	526, 831, 955
BmrFI	CCNGG	3 cut(s)	73, 464, 582
BmrI	ACTGGG	2 cut(s)	720, 1015
BmsI	GCATC	2 cut(s)	443, 463
BmuI	ACTGGG	2 cut(s)	720, 1015
BpiI	GAAGAC	3 cut(s)	302, 382, 732
BpmI	CTGGAG	1 cut(s)	1201
BpuEI	CTTGAG	1 cut(s)	146
BpuMI	CCSGG	1 cut(s)	464
BsaAI	YACGTR	1 cut(s)	1192
BsaBI	GATNNNNATC	1 cut(s)	573
BsaI	GGTCTC	1 cut(s)	402
BsaWI	WCCGGW	2 cut(s)	956, 998
BsaXI	ACNNNNNCTCC	6 cut(s)	264, 294, 536, 566, 728, 758
Bsc4I	CCNNNNNNNGG	3 cut(s)	248, 1005, 1072
Bse118I	RCCGGY	1 cut(s)	956
Bse1I	ACTGG	4 cut(s)	278, 715, 784, 1010
Bse8I	GATNNNNATC	1 cut(s)	573
BseBI	CCWGG	2 cut(s)	73, 582
BseGI	GGATG	6 cut(s)	68, 412, 487, 802, 973, 1082
BseJI	GATNNNNATC	1 cut(s)	573
BseLI	CCNNNNNNNGG	3 cut(s)	248, 1005, 1072
BseMII	CTCAG	1 cut(s)	888
BseNI	ACTGG	4 cut(s)	278, 715, 784, 1010
BseXI	GCAGC	3 cut(s)	481, 1012, 1063
BseYI	CCCAGC	1 cut(s)	846
BshFI	GGCC	3 cut(s)	76, 467, 630
BshNI	GGYRCC	1 cut(s)	953
BshTI	ACCGGT	1 cut(s)	956
BsiSI	CCGG	3 cut(s)	463, 957, 999
BslFI	GGGAC	1 cut(s)	266
BslI	CCNNNNNNNGG	3 cut(s)	248, 1005, 1072
BsmAI	GTCTC	3 cut(s)	100, 402, 1242
BsmFI	GGGAC	1 cut(s)	266
BsnI	GGCC	3 cut(s)	76, 467, 630
Bso31I	GGTCTC	1 cut(s)	402
Bsp1407I	TGTACA	1 cut(s)	885
Bsp143I	GATC	3 cut(s)	531, 568, 829
BspACI	CCGC	1 cut(s)	768
BspANI	GGCC	3 cut(s)	76, 467, 630
BspCNI	CTCAG	1 cut(s)	889
BspLI	GGNNCC	3 cut(s)	526, 831, 955
BspMI	ACCTGC	1 cut(s)	626
BspPI	GGATC	2 cut(s)	824, 837
BspT107I	GGYRCC	1 cut(s)	953
BspTI	CTTAAG	2 cut(s)	41, 170
BspTNI	GGTCTC	1 cut(s)	402
BsrFI	RCCGGY	1 cut(s)	956
BsrGI	TGTACA	1 cut(s)	885
BsrI	ACTGG	4 cut(s)	278, 715, 784, 1010
BssAI	RCCGGY	1 cut(s)	956
BssMI	GATC	3 cut(s)	531, 568, 829
Bst2UI	CCWGG	2 cut(s)	73, 582
Bst4CI	ACNGT	2 cut(s)	1039, 1169
Bst6I	CTCTTC	1 cut(s)	580
BstAFI	CTTAAG	2 cut(s)	41, 170
BstAUI	TGTACA	1 cut(s)	885
BstBAI	YACGTR	1 cut(s)	1192
BstC8I	GCNNGC	2 cut(s)	205, 1231
BstDEI	CTNAG	3 cut(s)	123, 224, 897
BstEII	GGTNACC	1 cut(s)	177
BstF5I	GGATG	6 cut(s)	68, 412, 487, 802, 973, 1082
BstKTI	GATC	3 cut(s)	534, 571, 832
BstMAI	GTCTC	3 cut(s)	100, 402, 1242
BstMBI	GATC	3 cut(s)	531, 568, 829
BstMWI	GCNNNNNNNGC	2 cut(s)	473, 1230
BstNI	CCWGG	2 cut(s)	73, 582
BstNSI	RCATGY	4 cut(s)	364, 387, 782, 985
BstPI	GGTNACC	1 cut(s)	177
BstSCI	CCNGG	3 cut(s)	71, 462, 580
BstSFI	CTRYAG	1 cut(s)	611
BstSNI	TACGTA	1 cut(s)	1192
BstV1I	GCAGC	3 cut(s)	481, 1012, 1063
BstV2I	GAAGAC	3 cut(s)	302, 382, 732
BstX2I	RGATCY	2 cut(s)	531, 829
BstYI	RGATCY	2 cut(s)	531, 829
BsuI	GTATCC	1 cut(s)	363
BsuRI	GGCC	3 cut(s)	76, 467, 630
BtsCI	GGATG	6 cut(s)	68, 412, 487, 802, 973, 1082
BveI	ACCTGC	1 cut(s)	626
Cac8I	GCNNGC	2 cut(s)	205, 1231
CaiI	CAGNNNCTG	1 cut(s)	284
Cfr10I	RCCGGY	1 cut(s)	956
Cfr13I	GGNCC	2 cut(s)	465, 628
CseI	GACGC	1 cut(s)	376
Csp6I	GTAC	4 cut(s)	435, 886, 954, 985
CspAI	ACCGGT	1 cut(s)	956
CviAII	CATG	8 cut(s)	192, 361, 384, 733, 755, 779, 936, 982
CviQI	GTAC	4 cut(s)	435, 886, 954, 985
DdeI	CTNAG	3 cut(s)	123, 224, 897
DpnI	GATC	3 cut(s)	533, 570, 831
DpnII	GATC	3 cut(s)	531, 568, 829
Eam1104I	CTCTTC	1 cut(s)	580
EarI	CTCTTC	1 cut(s)	580
Eco105I	TACGTA	1 cut(s)	1192
Eco31I	GGTCTC	1 cut(s)	402
Eco32I	GATATC	1 cut(s)	1019
Eco57I	CTGAAG	3 cut(s)	231, 306, 1014
Eco91I	GGTNACC	1 cut(s)	177
EcoO65I	GGTNACC	1 cut(s)	177
EcoRII	CCWGG	2 cut(s)	71, 580
EcoRV	GATATC	1 cut(s)	1019
FaeI	CATG	8 cut(s)	195, 364, 387, 736, 758, 782, 939, 985
FaqI	GGGAC	1 cut(s)	266
FatI	CATG	8 cut(s)	191, 360, 383, 732, 754, 778, 935, 981
FblI	GTMKAC	1 cut(s)	943
Fnu4HI	GCNGC	3 cut(s)	495, 1026, 1077
FokI	GGATG	6 cut(s)	55, 399, 494, 809, 980, 1069
Fsp4HI	GCNGC	3 cut(s)	495, 1026, 1077
FspBI	CTAG	2 cut(s)	314, 405
GluI	GCNGC	3 cut(s)	495, 1026, 1077
GsaI	CCCAGC	1 cut(s)	850
GsuI	CTGGAG	1 cut(s)	1201
HaeIII	GGCC	3 cut(s)	76, 467, 630
HapII	CCGG	3 cut(s)	463, 957, 999
HgaI	GACGC	1 cut(s)	376
Hin1II	CATG	8 cut(s)	195, 364, 387, 736, 758, 782, 939, 985
HincII	GTYRAC	1 cut(s)	944
HindII	GTYRAC	1 cut(s)	944
HinfI	GANTC	7 cut(s)	50, 257, 293, 307, 329, 510, 1235
HpaII	CCGG	3 cut(s)	463, 957, 999
HphI	GGTGA	6 cut(s)	17, 171, 338, 455, 1139, 1246
Hpy166II	GTNNAC	3 cut(s)	38, 944, 979
Hpy188I	TCNGA	6 cut(s)	211, 373, 417, 560, 858, 1033
Hpy188III	TCNNGA	9 cut(s)	54, 133, 518, 675, 761, 965, 1014, 1112, 1180
Hpy8I	GTNNAC	3 cut(s)	38, 944, 979
HpyAV	CCTTC	2 cut(s)	758, 1076
HpyCH4III	ACNGT	2 cut(s)	1039, 1169
HpyCH4IV	ACGT	2 cut(s)	1099, 1191
HpyCH4V	TGCA	4 cut(s)	203, 456, 497, 1229
HpyF10VI	GCNNNNNNNGC	2 cut(s)	473, 1230
HpyF3I	CTNAG	3 cut(s)	123, 224, 897
HpySE526I	ACGT	2 cut(s)	1099, 1191
Hsp92II	CATG	8 cut(s)	195, 364, 387, 736, 758, 782, 939, 985
KpnI	GGTACC	1 cut(s)	957
Kzo9I	GATC	3 cut(s)	531, 568, 829
LmnI	GCTCC	2 cut(s)	557, 736
Lsp1109I	GCAGC	3 cut(s)	481, 1012, 1063
LweI	GCATC	2 cut(s)	443, 463
MaeI	CTAG	2 cut(s)	314, 405
MaeII	ACGT	2 cut(s)	1099, 1191
MaeIII	GTNAC	5 cut(s)	177, 542, 772, 1091, 1252
MalI	GATC	3 cut(s)	533, 570, 831
MboI	GATC	3 cut(s)	531, 568, 829
MfeI	CAATTG	1 cut(s)	1148
MflI	RGATCY	2 cut(s)	531, 829
MluCI	AATT	5 cut(s)	154, 670, 881, 1107, 1148
MlyI	GAGTC	2 cut(s)	316, 1244
MmeI	TCCRAC	1 cut(s)	487
MnlI	CCTC	8 cut(s)	170, 245, 316, 409, 619, 812, 1096, 1189
MseI	TTAA	4 cut(s)	42, 171, 380, 1271
MslI	CAYNNNNRTG	1 cut(s)	560
MspCI	CTTAAG	2 cut(s)	41, 170
MspI	CCGG	3 cut(s)	463, 957, 999
MspR9I	CCNGG	3 cut(s)	73, 464, 582
MunI	CAATTG	1 cut(s)	1148
MvaI	CCWGG	2 cut(s)	73, 582
MwoI	GCNNNNNNNGC	2 cut(s)	473, 1230
NciI	CCSGG	1 cut(s)	464
NdeII	GATC	3 cut(s)	531, 568, 829
NlaIII	CATG	8 cut(s)	195, 364, 387, 736, 758, 782, 939, 985
NlaIV	GGNNCC	3 cut(s)	526, 831, 955
NmuCI	GTSAC	3 cut(s)	177, 1091, 1252
NspI	RCATGY	4 cut(s)	364, 387, 782, 985
PciI	ACATGT	3 cut(s)	360, 778, 981
PcsI	WCGNNNNNNNCGW	1 cut(s)	302
PfeI	GAWTC	5 cut(s)	50, 257, 293, 329, 510
PflMI	CCANNNNNTGG	1 cut(s)	248
PfoI	TCCNGGA	1 cut(s)	580
PinAI	ACCGGT	1 cut(s)	956
PkrI	GCNGC	3 cut(s)	496, 1027, 1078
PleI	GAGTC	2 cut(s)	315, 1243
PpsI	GAGTC	2 cut(s)	315, 1243
Ppu21I	YACGTR	1 cut(s)	1192
PscI	ACATGT	3 cut(s)	360, 778, 981
Psp6I	CCWGG	2 cut(s)	71, 580
PspEI	GGTNACC	1 cut(s)	177
PspFI	CCCAGC	1 cut(s)	846
PspGI	CCWGG	2 cut(s)	71, 580
PspN4I	GGNNCC	3 cut(s)	526, 831, 955
PspPI	GGNCC	2 cut(s)	465, 628
PstNI	CAGNNNCTG	1 cut(s)	284
PsuI	RGATCY	2 cut(s)	531, 829
RsaI	GTAC	4 cut(s)	436, 887, 955, 986
RsaNI	GTAC	4 cut(s)	435, 886, 954, 985
RseI	CAYNNNNRTG	1 cut(s)	560
SalI	GTCGAC	1 cut(s)	942
SaqAI	TTAA	4 cut(s)	42, 171, 380, 1271
SatI	GCNGC	3 cut(s)	495, 1026, 1077
Sau3AI	GATC	3 cut(s)	531, 568, 829
Sau96I	GGNCC	2 cut(s)	465, 628
ScaI	AGTACT	1 cut(s)	436
SchI	GAGTC	2 cut(s)	316, 1244
ScrFI	CCNGG	3 cut(s)	73, 464, 582
SfaNI	GCATC	2 cut(s)	443, 463
SfcI	CTRYAG	1 cut(s)	611
SmiMI	CAYNNNNRTG	1 cut(s)	560
SmlI	CTYRAG	3 cut(s)	41, 161, 170
SmoI	CTYRAG	3 cut(s)	41, 161, 170
SnaBI	TACGTA	1 cut(s)	1192
Sse9I	AATT	5 cut(s)	154, 670, 881, 1107, 1148
SsiI	CCGC	1 cut(s)	768
SspMI	CTAG	2 cut(s)	314, 405
StyD4I	CCNGG	3 cut(s)	71, 462, 580
TaaI	ACNGT	2 cut(s)	1039, 1169
TaiI	ACGT	2 cut(s)	1102, 1194
TaqI	TCGA	3 cut(s)	142, 451, 943
TasI	AATT	5 cut(s)	154, 670, 881, 1107, 1148
TatI	WGTACW	2 cut(s)	434, 885
TfiI	GAWTC	5 cut(s)	50, 257, 293, 329, 510
Tru1I	TTAA	4 cut(s)	42, 171, 380, 1271
Tru9I	TTAA	4 cut(s)	42, 171, 380, 1271
TseFI	GTSAC	3 cut(s)	177, 1091, 1252
TseI	GCWGC	3 cut(s)	494, 1025, 1076
Tsp45I	GTSAC	3 cut(s)	177, 1091, 1252
TspDTI	ATGAA	5 cut(s)	180, 224, 498, 563, 1227
Van91I	CCANNNNNTGG	1 cut(s)	248
Vha464I	CTTAAG	2 cut(s)	41, 170
XceI	RCATGY	4 cut(s)	364, 387, 782, 985
XmiI	GTMKAC	1 cut(s)	943
XspI	CTAG	2 cut(s)	314, 405
ZrmI	AGTACT	1 cut(s)	436

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.