Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0007972

Physical Location & Seq

Genomic Coordinates Forward (+)

38976515 .. 38980933

Gene Structure 4419 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1303200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1314 bp

ATGGCTGATCAGCAAGCCTCAATGGGCGAAGAAGAAGAGCCGCAGCAGCAGCAGCAGCAGCAACCCAATTCGCCTTCCCCAGACGCAGAGATTACAGATAACCCAACCCAGCAACAGCAAGAATACTCTTGGCCGGCGATTCGGTTCGATGTCCATCCGCAGAGAGCCTTCCACTTCCGCCGCCAGTTCCGAACTTCCCCGACCCCCAACAACTTTCTCAAGGGCGTCAAATGGTCGCCGGATGGATCGTGCTTTCTTACCAGTTCCGAGGACAACACGCTTCGGGTTTTTGCATTGCCAGATTATGGGAGTGAGAATGCAAATGGGTGTTCTGTAGCTGCTGATGAAGATTCGTATGCTGCAAACCTTGTTGTGAATGAGGGAGAGTCTGTATACGACTTCTGTTGGTACCCGTACATGTCTGCTGCAGACCCGGTTACCTGTGTTTTTGCAACTACATCTCGTGACCATCCAATTCATCTTTGGGATGCTTCTTCTGGACAGCTACGTTGCACTTATCGTGCTTATGATGCTGTGGATGAAATCACAGCTGCTTTTTCAATTGCCTTTAATCCTACTGGAACAAAGATTTTTGCTGGATACAACAAATCTGTTCGGGTGTTTGATTTACATCGCCCTGGTAGGGATTTTGAACAATATTCTACACTTCAAGGAAACAAAGAAGGACAAACAGGTATAATGTCTGCAATGGCATTTTGTCCAACCCATAGTGGAATGCTAGCTATAGGTTCTTATAGCAAGACTGCTGCAATATATAGGGAAGATAATATGGAACCCTTGTATGTATTGCATGGTCATGAAGGTGGGATTACACATGTCCAGTTTTCAAAAGATGGAAATTATTTATATACTGGAGGCCGAAAGGATCCTTATATATTATGCTGGGATATACGCAAAGCGGTTGATGTTGTGTATAAGTTGTATAGGTCATCGGAAGATACCAACCAGCGGATTTTGTTTGATATTGAGCCACTTGGACGCCATCTTTGTACAGGCGGTCAGGATGGTGTTGTGCATATCTATGATCTGCAAACTGGACAATGGGTAACAGGCTTCCAAGCGGCATTGGATACCGTTAACGGATTCTCCTTTCATCCCTTTCTGCCATTGGCCACCACTTCTTCAGGGCATCGAAGATTTGTTGGTCCTGATGATGGCAATGAGGAATTGCATCTGAGTGGTGACGAAAACTGCGCTTCTGTGTGGAGTTTCTCTGTTGCTTCAATGGTGGAGAATGATGCTGCAATGGACGCCGATGATTATAACAGCCACAACCTCGACCAGCATCCCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005697 GO:0006996 GO:0008150 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010556 GO:0010557 GO:0010604 GO:0015030 GO:0016043 GO:0016604 GO:0019219 GO:0019222 GO:0022607 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031974 GO:0031981 GO:0032200 GO:0032202 GO:0032203 GO:0032991 GO:0043085 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044085 GO:0044093 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045935 GO:0048518 GO:0048522 GO:0050789 GO:0050790 GO:0050794 GO:0051052 GO:0051054 GO:0051171 GO:0051173 GO:0051276 GO:0051338 GO:0051347 GO:0051972 GO:0051973 GO:0060255 GO:0065007 GO:0065009 GO:0070013 GO:0071840 GO:0080090 GO:0097159 GO:1901363 GO:1990904 GO:2000112 GO:2000278 GO:2000573

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

438

Amino Acids

48.82

Weight (kDa)

4.78

Isoelectric Point (pI)

46.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_THOC3	PF25174	74 - 373	3.3e-25	THOC3 beta-propeller domain
Beta-prop_WDR5	PF25175	75 - 211	4e-06	WDR5 beta-propeller domain
Beta-prop_EML_2	PF23414	142 - 307	2.9e-14	Echinoderm microtubule-associated protein second beta-propeller
EIF3I	PF24805	144 - 306	5.1e-06	EIF3I
WD40_Prp19	PF24814	148 - 373	2e-15	Prp19 WD40 domain
Beta-prop_EML	PF23409	149 - 304	7.4e-06	Echinoderm microtubule-associated protein first beta-propeller
WD40_WDHD1_1st	PF24817	151 - 310	2.3e-08	WDHD1 first WD40 domain
Beta-prop_WDR3_1st	PF25173	203 - 373	1.9e-13	WDR3 first beta-propeller domain
Beta-prop_TEP1_2nd	PF25047	217 - 362	1.3e-11	TEP-1 second beta-propeller
Beta-prop_WDR5	PF25175	229 - 382	1.5e-14	WDR5 beta-propeller domain
WDR55	PF24796	236 - 371	2.1e-08	WDR55
WD40	PF00400	265 - 303	3.8e-07	WD domain, G-beta repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0007972)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G21520 AT4G21520
fragaria_vesca	FvH4_3g04030 FvH4_3g04030 FvH4_3g04030 FvH4_3g04030 FvH4_3g04030 FvH4_3g04030
malus_domestica	MD05G1325300.v1.1 MD10G1303200.v1.1
prunus_persica	Prupe.4G036600_v2.0.a1
pyrus_communis	pycom05g29930 pycom10g25570
rosa_chinensis	RchiOBHm_Chr5g0006371
rosa_laevigata	RLG00000031362
rosa_multiflora	Rmu_sc0037704.1_g000001
rosa_roxburghii	Rroxscaffold_1G00069560
rosa_rugosa	Rorug04G0421200 Rorug04G0421200
rosa_samantha	Rh5AG049200 Rh5BG048500 Rh5CG056700 Rh5DG047800
rosa_wichuraiana	Rw5G004900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1284
Acc65I	GGTACC	1 cut(s)	408
AccB1I	GGYRCC	1 cut(s)	408
AccB7I	CCANNNNNTGG	1 cut(s)	305
AccI	GTMKAC	1 cut(s)	393
AciI	CCGC	8 cut(s)	41, 158, 178, 181, 920, 970, 1017, 1082
AclWI	GGATC	3 cut(s)	253, 881, 894
AcoI	YGGCCR	2 cut(s)	131, 1131
AcuI	CTGAAG	1 cut(s)	1128
AcyI	GRCGYC	3 cut(s)	225, 1000, 1272
AfaI	GTAC	3 cut(s)	410, 416, 1012
AfiI	CCNNNNNNNGG	4 cut(s)	305, 643, 969, 1175
AflIII	ACRYGT	2 cut(s)	417, 835
AgsI	TTSAA	5 cut(s)	561, 653, 671, 849, 1245
AjnI	CCWGG	1 cut(s)	637
AluBI	AGCT	4 cut(s)	338, 505, 551, 743
AluI	AGCT	4 cut(s)	338, 505, 551, 743
AlwI	GGATC	3 cut(s)	253, 881, 894
AoxI	GGCC	3 cut(s)	131, 877, 1131
ArsI	GACNNNNNNTTYG	2 cut(s)	990, 1022
Asp718I	GGTACC	1 cut(s)	408
AspLEI	GCGC	1 cut(s)	1217
AspS9I	GGNCC	1 cut(s)	1166
AsuC2I	CCSGG	1 cut(s)	434
AsuHPI	GGTGA	1 cut(s)	1214
AsuNHI	GCTAGC	1 cut(s)	739
AvaII	GGWCC	1 cut(s)	1166
BalI	TGGCCA	1 cut(s)	1133
BamHI	GGATCC	1 cut(s)	886
BanI	GGYRCC	1 cut(s)	408
BauI	CACGAG	1 cut(s)	462
BccI	CCATC	7 cut(s)	162, 236, 477, 848, 1011, 1019, 1169
BciT130I	CCWGG	1 cut(s)	639
BciVI	GTATCC	2 cut(s)	593, 1084
BclI	TGATCA	1 cut(s)	7
BcnI	CCSGG	1 cut(s)	434
BfaI	CTAG	2 cut(s)	740, 1312
BfmI	CTRYAG	3 cut(s)	333, 426, 744
BfuI	GTATCC	2 cut(s)	593, 1084
Bme1390I	CCNGG	2 cut(s)	434, 639
Bme18I	GGWCC	1 cut(s)	1166
BmgT120I	GGNCC	1 cut(s)	1166
BmiI	GGNNCC	3 cut(s)	410, 795, 888
BmrFI	CCNGG	2 cut(s)	434, 639
BmsI	GCATC	5 cut(s)	478, 520, 1159, 1201, 1249
BmtI	GCTAGC	1 cut(s)	743
BpmI	CTGGAG	1 cut(s)	894
BpuEI	CTTGAG	1 cut(s)	203
BpuMI	CCSGG	1 cut(s)	434
BsaBI	GATNNNNATC	2 cut(s)	153, 630
BsaHI	GRCGYC	3 cut(s)	225, 1000, 1272
BsaJI	CCNNGG	2 cut(s)	267, 637
BsaXI	ACNNNNNCTCC	4 cut(s)	375, 405, 1091, 1121
Bsc4I	CCNNNNNNNGG	4 cut(s)	305, 643, 969, 1175
Bse118I	RCCGGY	1 cut(s)	133
Bse1I	ACTGG	6 cut(s)	184, 261, 583, 841, 877, 1060
Bse3DI	GCAATG	4 cut(s)	293, 714, 1186, 1272
Bse8I	GATNNNNATC	2 cut(s)	153, 630
BseBI	CCWGG	1 cut(s)	639
BseDI	CCNNGG	2 cut(s)	267, 637
BseGI	GGATG	8 cut(s)	154, 247, 469, 493, 544, 1030, 1114, 1306
BseJI	GATNNNNATC	2 cut(s)	153, 630
BseLI	CCNNNNNNNGG	4 cut(s)	305, 643, 969, 1175
BseMI	GCAATG	4 cut(s)	293, 714, 1186, 1272
BseMII	CTCAG	1 cut(s)	1187
BseNI	ACTGG	6 cut(s)	184, 261, 583, 841, 877, 1060
BseYI	CCCAGC	2 cut(s)	108, 903
BshFI	GGCC	3 cut(s)	133, 879, 1133
BshNI	GGYRCC	1 cut(s)	408
BsiSI	CCGG	3 cut(s)	134, 239, 434
BslI	CCNNNNNNNGG	4 cut(s)	305, 643, 969, 1175
BsmI	GAATGC	2 cut(s)	322, 741
BsnI	GGCC	3 cut(s)	133, 879, 1133
Bsp1407I	TGTACA	1 cut(s)	1010
Bsp143I	GATC	4 cut(s)	7, 245, 886, 1045
BspACI	CCGC	8 cut(s)	41, 158, 178, 181, 920, 970, 1017, 1082
BspANI	GGCC	3 cut(s)	133, 879, 1133
BspCNI	CTCAG	1 cut(s)	1188
BspHI	TCATGA	1 cut(s)	817
BspLI	GGNNCC	3 cut(s)	410, 795, 888
BspMAI	CTGCAG	1 cut(s)	430
BspOI	GCTAGC	1 cut(s)	743
BspPI	GGATC	3 cut(s)	253, 881, 894
BspQI	GCTCTTC	1 cut(s)	30
BspT107I	GGYRCC	1 cut(s)	408
BsrDI	GCAATG	4 cut(s)	293, 714, 1186, 1272
BsrFI	RCCGGY	1 cut(s)	133
BsrGI	TGTACA	1 cut(s)	1010
BsrI	ACTGG	6 cut(s)	184, 261, 583, 841, 877, 1060
BssAI	RCCGGY	1 cut(s)	133
BssECI	CCNNGG	2 cut(s)	267, 637
BssMI	GATC	4 cut(s)	7, 245, 886, 1045
BssNAI	GTATAC	1 cut(s)	394
BssNI	GRCGYC	3 cut(s)	225, 1000, 1272
BssSI	CACGAG	1 cut(s)	462
Bst1107I	GTATAC	1 cut(s)	394
Bst2BI	CACGAG	1 cut(s)	462
Bst2UI	CCWGG	1 cut(s)	639
Bst4CI	ACNGT	1 cut(s)	1096
Bst6I	CTCTTC	1 cut(s)	30
BstACI	GRCGYC	3 cut(s)	225, 1000, 1272
BstAUI	TGTACA	1 cut(s)	1010
BstC8I	GCNNGC	3 cut(s)	15, 135, 741
BstDEI	CTNAG	1 cut(s)	1196
BstEII	GGTNACC	1 cut(s)	436
BstF5I	GGATG	8 cut(s)	154, 247, 469, 493, 544, 1030, 1114, 1306
BstHHI	GCGC	1 cut(s)	1217
BstKTI	GATC	4 cut(s)	10, 248, 889, 1048
BstMBI	GATC	4 cut(s)	7, 245, 886, 1045
BstMWI	GCNNNNNNNGC	7 cut(s)	46, 49, 52, 55, 58, 530, 1271
BstNI	CCWGG	1 cut(s)	639
BstNSI	RCATGY	2 cut(s)	421, 839
BstPI	GGTNACC	1 cut(s)	436
BstSCI	CCNGG	2 cut(s)	432, 637
BstSFI	CTRYAG	3 cut(s)	333, 426, 744
BstX2I	RGATCY	1 cut(s)	886
BstYI	RGATCY	1 cut(s)	886
BstZ17I	GTATAC	1 cut(s)	394
BsuI	GTATCC	2 cut(s)	593, 1084
BsuRI	GGCC	3 cut(s)	133, 879, 1133
BtgZI	GCGATG	1 cut(s)	617
BtsCI	GGATG	8 cut(s)	154, 247, 469, 493, 544, 1030, 1114, 1306
Cac8I	GCNNGC	3 cut(s)	15, 135, 741
CciI	TCATGA	1 cut(s)	817
CfoI	GCGC	1 cut(s)	1217
Cfr10I	RCCGGY	1 cut(s)	133
Cfr13I	GGNCC	1 cut(s)	1166
CseI	GACGC	4 cut(s)	92, 214, 1008, 1280
Csp6I	GTAC	3 cut(s)	409, 415, 1011
CviAII	CATG	4 cut(s)	418, 812, 818, 836
CviQI	GTAC	3 cut(s)	409, 415, 1011
DdeI	CTNAG	1 cut(s)	1196
DpnI	GATC	4 cut(s)	9, 247, 888, 1047
DpnII	GATC	4 cut(s)	7, 245, 886, 1045
EaeI	YGGCCR	2 cut(s)	131, 1131
Eam1104I	CTCTTC	1 cut(s)	30
EarI	CTCTTC	1 cut(s)	30
EciI	GGCGGA	1 cut(s)	167
Eco47I	GGWCC	1 cut(s)	1166
Eco57I	CTGAAG	1 cut(s)	1128
Eco91I	GGTNACC	1 cut(s)	436
EcoO65I	GGTNACC	1 cut(s)	436
EcoRII	CCWGG	1 cut(s)	637
FaeI	CATG	4 cut(s)	421, 815, 821, 839
FatI	CATG	4 cut(s)	417, 811, 817, 835
FbaI	TGATCA	1 cut(s)	7
FblI	GTMKAC	1 cut(s)	393
FokI	GGATG	8 cut(s)	141, 254, 456, 500, 551, 1037, 1101, 1293
FspBI	CTAG	2 cut(s)	740, 1312
GlaI	GCGC	1 cut(s)	1216
GsaI	CCCAGC	2 cut(s)	112, 907
GsuI	CTGGAG	1 cut(s)	894
HaeIII	GGCC	3 cut(s)	133, 879, 1133
HapII	CCGG	3 cut(s)	134, 239, 434
HgaI	GACGC	4 cut(s)	92, 214, 1008, 1280
HhaI	GCGC	1 cut(s)	1217
Hin1I	GRCGYC	3 cut(s)	225, 1000, 1272
Hin1II	CATG	4 cut(s)	421, 815, 821, 839
Hin6I	GCGC	1 cut(s)	1215
HinP1I	GCGC	1 cut(s)	1215
HincII	GTYRAC	1 cut(s)	1099
HindII	GTYRAC	1 cut(s)	1099
HinfI	GANTC	4 cut(s)	139, 350, 386, 1104
HpaI	GTTAAC	1 cut(s)	1099
HpaII	CCGG	3 cut(s)	134, 239, 434
HphI	GGTGA	1 cut(s)	1214
Hpy166II	GTNNAC	2 cut(s)	394, 1099
Hpy188I	TCNGA	4 cut(s)	191, 268, 955, 1197
Hpy188III	TCNNGA	5 cut(s)	464, 498, 818, 1022, 1169
Hpy8I	GTNNAC	2 cut(s)	394, 1099
HpyAV	CCTTC	4 cut(s)	84, 178, 677, 815
HpyCH4III	ACNGT	1 cut(s)	1096
HpyCH4IV	ACGT	1 cut(s)	508
HpyF10VI	GCNNNNNNNGC	7 cut(s)	46, 49, 52, 55, 58, 530, 1271
HpyF3I	CTNAG	1 cut(s)	1196
HpySE526I	ACGT	1 cut(s)	508
Hsp92I	GRCGYC	3 cut(s)	225, 1000, 1272
Hsp92II	CATG	4 cut(s)	421, 815, 821, 839
HspAI	GCGC	1 cut(s)	1215
KpnI	GGTACC	1 cut(s)	412
KroI	GCCGGC	1 cut(s)	133
KroNI	GCCGGC	1 cut(s)	135
Ksp22I	TGATCA	1 cut(s)	7
KspAI	GTTAAC	1 cut(s)	1099
Kzo9I	GATC	4 cut(s)	7, 245, 886, 1045
LguI	GCTCTTC	1 cut(s)	30
LweI	GCATC	5 cut(s)	478, 520, 1159, 1201, 1249
MaeI	CTAG	2 cut(s)	740, 1312
MaeII	ACGT	1 cut(s)	508
MaeIII	GTNAC	4 cut(s)	436, 464, 1066, 1202
MalI	GATC	4 cut(s)	9, 247, 888, 1047
MboI	GATC	4 cut(s)	7, 245, 886, 1045
MboII	GAAGA	9 cut(s)	41, 44, 47, 359, 486, 794, 968, 1134, 1167
MfeI	CAATTG	1 cut(s)	561
MflI	RGATCY	1 cut(s)	886
MlsI	TGGCCA	1 cut(s)	1133
MluCI	AATT	5 cut(s)	67, 474, 561, 859, 1187
MluNI	TGGCCA	1 cut(s)	1133
MlyI	GAGTC	1 cut(s)	395
MmeI	TCCRAC	1 cut(s)	746
MnlI	CCTC	6 cut(s)	28, 262, 373, 869, 1177, 1307
Mox20I	TGGCCA	1 cut(s)	1133
MroNI	GCCGGC	1 cut(s)	133
MscI	TGGCCA	1 cut(s)	1133
MseI	TTAA	2 cut(s)	570, 1098
MslI	CAYNNNNRTG	4 cut(s)	816, 822, 1041, 1197
Msp20I	TGGCCA	1 cut(s)	1133
MspA1I	CMGCKG	2 cut(s)	551, 970
MspI	CCGG	3 cut(s)	134, 239, 434
MspR9I	CCNGG	2 cut(s)	434, 639
MunI	CAATTG	1 cut(s)	561
Mva1269I	GAATGC	2 cut(s)	322, 741
MvaI	CCWGG	1 cut(s)	639
MwoI	GCNNNNNNNGC	7 cut(s)	46, 49, 52, 55, 58, 530, 1271
NaeI	GCCGGC	1 cut(s)	135
NciI	CCSGG	1 cut(s)	434
NdeII	GATC	4 cut(s)	7, 245, 886, 1045
NgoMIV	GCCGGC	1 cut(s)	133
NheI	GCTAGC	1 cut(s)	739
NlaIII	CATG	4 cut(s)	421, 815, 821, 839
NlaIV	GGNNCC	3 cut(s)	410, 795, 888
NmuCI	GTSAC	2 cut(s)	464, 1202
NspI	RCATGY	2 cut(s)	421, 839
PagI	TCATGA	1 cut(s)	817
PciI	ACATGT	2 cut(s)	417, 835
PciSI	GCTCTTC	1 cut(s)	30
PctI	GAATGC	2 cut(s)	322, 741
PdiI	GCCGGC	1 cut(s)	135
PfeI	GAWTC	3 cut(s)	139, 350, 1104
PflMI	CCANNNNNTGG	1 cut(s)	305
PleI	GAGTC	1 cut(s)	394
PpsI	GAGTC	1 cut(s)	394
PscI	ACATGT	2 cut(s)	417, 835
PsiI	TTATAA	1 cut(s)	1284
Psp6I	CCWGG	1 cut(s)	637
PspEI	GGTNACC	1 cut(s)	436
PspFI	CCCAGC	2 cut(s)	108, 903
PspGI	CCWGG	1 cut(s)	637
PspN4I	GGNNCC	3 cut(s)	410, 795, 888
PspPI	GGNCC	1 cut(s)	1166
PstI	CTGCAG	1 cut(s)	430
PsuI	RGATCY	1 cut(s)	886
PvuII	CAGCTG	1 cut(s)	551
RsaI	GTAC	3 cut(s)	410, 416, 1012
RsaNI	GTAC	3 cut(s)	409, 415, 1011
RseI	CAYNNNNRTG	4 cut(s)	816, 822, 1041, 1197
SapI	GCTCTTC	1 cut(s)	30
SaqAI	TTAA	2 cut(s)	570, 1098
Sau3AI	GATC	4 cut(s)	7, 245, 886, 1045
Sau96I	GGNCC	1 cut(s)	1166
SchI	GAGTC	1 cut(s)	395
ScrFI	CCNGG	2 cut(s)	434, 639
SfaNI	GCATC	5 cut(s)	478, 520, 1159, 1201, 1249
SfcI	CTRYAG	3 cut(s)	333, 426, 744
SinI	GGWCC	1 cut(s)	1166
SmiMI	CAYNNNNRTG	4 cut(s)	816, 822, 1041, 1197
SmlI	CTYRAG	1 cut(s)	218
SmoI	CTYRAG	1 cut(s)	218
Sse9I	AATT	5 cut(s)	67, 474, 561, 859, 1187
SsiI	CCGC	8 cut(s)	41, 158, 178, 181, 920, 970, 1017, 1082
SspI	AATATT	1 cut(s)	659
SspMI	CTAG	2 cut(s)	740, 1312
StyD4I	CCNGG	2 cut(s)	432, 637
TaaI	ACNGT	1 cut(s)	1096
TaiI	ACGT	1 cut(s)	511
TaqI	TCGA	3 cut(s)	147, 1153, 1299
TasI	AATT	5 cut(s)	67, 474, 561, 859, 1187
TatI	WGTACW	1 cut(s)	1010
TauI	GCSGC	3 cut(s)	43, 183, 1085
TfiI	GAWTC	3 cut(s)	139, 350, 1104
Tru1I	TTAA	2 cut(s)	570, 1098
Tru9I	TTAA	2 cut(s)	570, 1098
TseFI	GTSAC	2 cut(s)	464, 1202
Tsp45I	GTSAC	2 cut(s)	464, 1202
TspDTI	ATGAA	5 cut(s)	360, 467, 555, 834, 1103
TspGWI	ACGGA	1 cut(s)	1116
Van91I	CCANNNNNTGG	1 cut(s)	305
VpaK11BI	GGWCC	1 cut(s)	1166
XceI	RCATGY	2 cut(s)	421, 839
XcmI	CCANNNNNNNNNTGG	1 cut(s)	480
XmiI	GTMKAC	1 cut(s)	393
XspI	CTAG	2 cut(s)	740, 1312

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.