Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0007972

Physical Location & Seq

Genomic Coordinates Forward (+)

45152164 .. 45155702

Gene Structure 3539 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1325300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1296 bp

ATGGCCGAAGAAGAAGAGCGGCAGCAGCACCAACCCAATTCGCCTTCCCCAGACGCAGAAATTACAGAACACCCAACCCAGCAACAGCAAGACGGCTACTCTTGGCCGGCGATTCGGTTCGATGTTCATCCGCACAGAGCCTTCCACTTCCGCCACCAGTTCCGAACTTCCCCAATTCCCAACAGCTTTCTCAAGGGCGTCACATGGTCGCCGGATGGGTCGTGCTTTCTTACCAGTTCCGAGGACAACACGCTTCGGGTTTTCGCATTACCAGATTATGGGAGTGAGAATGATCATGGGTGTTCTGTAGCTGCTGATGAAGATTCGTATGCTGCAAACCTTGTTGTGAACGAGGGAGAGTCTGTATACGACTTCTGTTGGTACCCATACATGTCTGCTTCAGACCCGGTTACCTGTGTTTTTGCAACTACATCTCGTGACCATCCCATTCATCTTTGGGATGCTTCTTCTGGACAGCTACGTTGCACTTATCGTGCTTATGATGCTGTGGATGAAATCACGGCTGCTTTTTCAATTGCCTTTCCTTTTCAATATTCTACTGGAACAAAGATTTTTGCTGGATACAACAAATCCGTTCGGGTGTTTGATTTACATAACCCTGGTAGGGATTTTGAACAATATTCGACACTTCAAGGAAACAAAGAAGGCCAAACGGGTATAGTGTCCGCAATGTCTTTTTGTCCAACCCATAGTGGAATGCTTGCTATAGGTTCTTACAGCAAAACTGCTGCAATATATAGCGAAGATAATATGGAACCCTTGTATGTATTGCATGGTCATGAAGGTGGGATTACACATGTCCAGTTTTCAAAGGATGGAAATTATTTATATACTGGAGCCCGAAAGGATCCTTATATATTATGCTGGGATATACGCAAAGCAGTTGATGTTGTGTACAAGTTATATAGGTCATCGGAAGATACCAACCAACGGATTTTGTTTGATATTGAGCCACTTGGACGCCATCTTTGTACAGGTGGTCAGGACGGTGTTGTGCATATCTATGATCTGCAAACTGGACAATGGGTATCAGGCTTCCAAGCGGCATTGGATACTGTTAATGGAATCTCCTTTCATCCCTTTCTGCCAATGGCTACCACTTCTTCAGGGCATCGAAGATTTGTTGATCCTGATGGTGGCAATGACAAATTGCATCTGAGTGGTGATGAAAACTGTGCTTCTGTGTGGAGTTTCTCTGTTGCTTCAATGGTGGAGAATGATGGTGCAATGGACGGTGATGATTATAACAGCCACAACCTTGACCAGCATCCCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005697 GO:0006996 GO:0008150 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010556 GO:0010557 GO:0010604 GO:0015030 GO:0016043 GO:0016604 GO:0019219 GO:0019222 GO:0022607 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031974 GO:0031981 GO:0032200 GO:0032202 GO:0032203 GO:0032991 GO:0043085 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044085 GO:0044093 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045935 GO:0048518 GO:0048522 GO:0050789 GO:0050790 GO:0050794 GO:0051052 GO:0051054 GO:0051171 GO:0051173 GO:0051276 GO:0051338 GO:0051347 GO:0051972 GO:0051973 GO:0060255 GO:0065007 GO:0065009 GO:0070013 GO:0071840 GO:0080090 GO:0097159 GO:1901363 GO:1990904 GO:2000112 GO:2000278 GO:2000573

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

432

Amino Acids

48.12

Weight (kDa)

4.92

Isoelectric Point (pI)

42.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_THOC3	PF25174	65 - 301	1.2e-16	THOC3 beta-propeller domain
Beta-prop_EML_2	PF23414	134 - 301	5.9e-12	Echinoderm microtubule-associated protein second beta-propeller
EIF3I	PF24805	136 - 301	4e-06	EIF3I
WD40_Prp19	PF24814	140 - 322	1.6e-09	Prp19 WD40 domain
WD40_CDC20-Fz	PF24807	184 - 371	5.4e-12	CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st	PF25173	193 - 367	6e-14	WDR3 first beta-propeller domain
WD40_Prp19	PF24814	195 - 365	1.1e-13	Prp19 WD40 domain
WD40_WDHD1_1st	PF24817	198 - 304	2.2e-06	WDHD1 first WD40 domain
Beta-prop_TEP1_2nd	PF25047	211 - 362	1.2e-11	TEP-1 second beta-propeller
Beta-prop_WDR5	PF25175	222 - 376	2.2e-16	WDR5 beta-propeller domain
WDR55	PF24796	228 - 368	2e-09	WDR55
WD40	PF00400	259 - 297	6.6e-07	WD domain, G-beta repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0007972)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G21520 AT4G21520
fragaria_vesca	FvH4_3g04030 FvH4_3g04030 FvH4_3g04030 FvH4_3g04030 FvH4_3g04030 FvH4_3g04030
malus_domestica	MD05G1325300.v1.1 MD10G1303200.v1.1
prunus_persica	Prupe.4G036600_v2.0.a1
pyrus_communis	pycom05g29930 pycom10g25570
rosa_chinensis	RchiOBHm_Chr5g0006371
rosa_laevigata	RLG00000031362
rosa_multiflora	Rmu_sc0037704.1_g000001
rosa_roxburghii	Rroxscaffold_1G00069560
rosa_rugosa	Rorug04G0421200 Rorug04G0421200
rosa_samantha	Rh5AG049200 Rh5BG048500 Rh5CG056700 Rh5DG047800
rosa_wichuraiana	Rw5G004900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1266
Acc65I	GGTACC	1 cut(s)	381
AccB1I	GGYRCC	1 cut(s)	381
AccB7I	CCANNNNNTGG	1 cut(s)	278
AccBSI	CCGCTC	1 cut(s)	19
AccI	GTMKAC	1 cut(s)	366
AciI	CCGC	5 cut(s)	19, 131, 151, 687, 1064
AclWI	GGATC	3 cut(s)	863, 876, 1142
AcoI	YGGCCR	2 cut(s)	3, 104
AcuI	CTGAAG	2 cut(s)	384, 1110
AcyI	GRCGYC	2 cut(s)	198, 982
AfaI	GTAC	3 cut(s)	383, 917, 994
AfiI	CCNNNNNNNGG	4 cut(s)	278, 625, 951, 1157
AflIII	ACRYGT	2 cut(s)	390, 817
AgsI	TTSAA	6 cut(s)	534, 551, 635, 653, 831, 1227
AjnI	CCWGG	1 cut(s)	619
AluBI	AGCT	3 cut(s)	186, 311, 478
AluI	AGCT	3 cut(s)	186, 311, 478
AlwI	GGATC	3 cut(s)	863, 876, 1142
AoxI	GGCC	3 cut(s)	3, 104, 667
ApeKI	GCWGC	6 cut(s)	22, 25, 311, 332, 524, 749
ArsI	GACNNNNNNTTYG	2 cut(s)	972, 1004
Asp718I	GGTACC	1 cut(s)	381
AsuC2I	CCSGG	1 cut(s)	407
AsuHPI	GGTGA	2 cut(s)	1196, 1268
BamHI	GGATCC	1 cut(s)	868
BanI	GGYRCC	1 cut(s)	381
BanII	GRGCYC	1 cut(s)	862
BauI	CACGAG	1 cut(s)	435
BbvI	GCAGC	6 cut(s)	34, 37, 298, 319, 511, 736
BccI	CCATC	6 cut(s)	209, 450, 830, 993, 1148, 1235
BceAI	ACGGC	2 cut(s)	109, 537
BciT130I	CCWGG	1 cut(s)	621
BciVI	GTATCC	2 cut(s)	575, 1066
BclI	TGATCA	1 cut(s)	292
BcnI	CCSGG	1 cut(s)	407
BfaI	CTAG	1 cut(s)	1294
BfmI	CTRYAG	2 cut(s)	306, 726
BfuI	GTATCC	2 cut(s)	575, 1066
BisI	GCNGC	8 cut(s)	20, 23, 26, 312, 333, 525, 750, 1065
BlsI	GCNGC	8 cut(s)	21, 24, 27, 313, 334, 526, 751, 1066
Bme1390I	CCNGG	2 cut(s)	407, 621
BmiI	GGNNCC	4 cut(s)	383, 777, 859, 870
BmrFI	CCNGG	2 cut(s)	407, 621
BmsI	GCATC	4 cut(s)	451, 493, 1141, 1183
BpmI	CTGGAG	1 cut(s)	876
BpuEI	CTTGAG	1 cut(s)	176
BpuMI	CCSGG	1 cut(s)	407
BsaBI	GATNNNNATC	1 cut(s)	126
BsaHI	GRCGYC	2 cut(s)	198, 982
BsaJI	CCNNGG	2 cut(s)	240, 619
BsaXI	ACNNNNNCTCC	2 cut(s)	348, 378
Bsc4I	CCNNNNNNNGG	4 cut(s)	278, 625, 951, 1157
Bse118I	RCCGGY	1 cut(s)	106
Bse1I	ACTGG	6 cut(s)	157, 234, 565, 823, 859, 1042
Bse3DI	GCAATG	3 cut(s)	696, 1168, 1254
Bse8I	GATNNNNATC	1 cut(s)	126
BseBI	CCWGG	1 cut(s)	621
BseDI	CCNNGG	2 cut(s)	240, 619
BseGI	GGATG	8 cut(s)	127, 220, 442, 466, 517, 841, 1096, 1288
BseJI	GATNNNNATC	1 cut(s)	126
BseLI	CCNNNNNNNGG	4 cut(s)	278, 625, 951, 1157
BseMI	GCAATG	3 cut(s)	696, 1168, 1254
BseMII	CTCAG	1 cut(s)	1169
BseNI	ACTGG	6 cut(s)	157, 234, 565, 823, 859, 1042
BseXI	GCAGC	6 cut(s)	34, 37, 298, 319, 511, 736
BseYI	CCCAGC	2 cut(s)	78, 885
BshFI	GGCC	3 cut(s)	5, 106, 669
BshNI	GGYRCC	1 cut(s)	381
BsiSI	CCGG	3 cut(s)	107, 212, 407
BslI	CCNNNNNNNGG	4 cut(s)	278, 625, 951, 1157
BsmI	GAATGC	1 cut(s)	723
BsnI	GGCC	3 cut(s)	5, 106, 669
Bsp1286I	GDGCHC	1 cut(s)	862
Bsp1407I	TGTACA	2 cut(s)	915, 992
Bsp143I	GATC	4 cut(s)	292, 868, 1027, 1147
BspACI	CCGC	5 cut(s)	19, 131, 151, 687, 1064
BspANI	GGCC	3 cut(s)	5, 106, 669
BspCNI	CTCAG	1 cut(s)	1170
BspHI	TCATGA	1 cut(s)	799
BspLI	GGNNCC	4 cut(s)	383, 777, 859, 870
BspPI	GGATC	3 cut(s)	863, 876, 1142
BspQI	GCTCTTC	1 cut(s)	9
BspT107I	GGYRCC	1 cut(s)	381
BsrBI	CCGCTC	1 cut(s)	19
BsrDI	GCAATG	3 cut(s)	696, 1168, 1254
BsrFI	RCCGGY	1 cut(s)	106
BsrGI	TGTACA	2 cut(s)	915, 992
BsrI	ACTGG	6 cut(s)	157, 234, 565, 823, 859, 1042
BssAI	RCCGGY	1 cut(s)	106
BssECI	CCNNGG	2 cut(s)	240, 619
BssMI	GATC	4 cut(s)	292, 868, 1027, 1147
BssNAI	GTATAC	1 cut(s)	367
BssNI	GRCGYC	2 cut(s)	198, 982
BssSI	CACGAG	1 cut(s)	435
Bst1107I	GTATAC	1 cut(s)	367
Bst2BI	CACGAG	1 cut(s)	435
Bst2UI	CCWGG	1 cut(s)	621
Bst4CI	ACNGT	4 cut(s)	1010, 1078, 1196, 1256
Bst6I	CTCTTC	1 cut(s)	9
BstACI	GRCGYC	2 cut(s)	198, 982
BstAUI	TGTACA	2 cut(s)	915, 992
BstC8I	GCNNGC	2 cut(s)	108, 723
BstDEI	CTNAG	1 cut(s)	1178
BstEII	GGTNACC	1 cut(s)	409
BstF5I	GGATG	8 cut(s)	127, 220, 442, 466, 517, 841, 1096, 1288
BstKTI	GATC	4 cut(s)	295, 871, 1030, 1150
BstMBI	GATC	4 cut(s)	292, 868, 1027, 1147
BstMWI	GCNNNNNNNGC	2 cut(s)	25, 503
BstNI	CCWGG	1 cut(s)	621
BstNSI	RCATGY	2 cut(s)	394, 821
BstPI	GGTNACC	1 cut(s)	409
BstSCI	CCNGG	2 cut(s)	405, 619
BstSFI	CTRYAG	2 cut(s)	306, 726
BstV1I	GCAGC	6 cut(s)	34, 37, 298, 319, 511, 736
BstX2I	RGATCY	1 cut(s)	868
BstYI	RGATCY	1 cut(s)	868
BstZ17I	GTATAC	1 cut(s)	367
BsuI	GTATCC	2 cut(s)	575, 1066
BsuRI	GGCC	3 cut(s)	5, 106, 669
BtsCI	GGATG	8 cut(s)	127, 220, 442, 466, 517, 841, 1096, 1288
Cac8I	GCNNGC	2 cut(s)	108, 723
CciI	TCATGA	1 cut(s)	799
Cfr10I	RCCGGY	1 cut(s)	106
CseI	GACGC	3 cut(s)	62, 187, 990
Csp6I	GTAC	3 cut(s)	382, 916, 993
CviAII	CATG	6 cut(s)	204, 296, 391, 794, 800, 818
CviQI	GTAC	3 cut(s)	382, 916, 993
DdeI	CTNAG	1 cut(s)	1178
DpnI	GATC	4 cut(s)	294, 870, 1029, 1149
DpnII	GATC	4 cut(s)	292, 868, 1027, 1147
EaeI	YGGCCR	2 cut(s)	3, 104
Eam1104I	CTCTTC	1 cut(s)	9
EarI	CTCTTC	1 cut(s)	9
EciI	GGCGGA	1 cut(s)	140
Eco24I	GRGCYC	1 cut(s)	862
Eco57I	CTGAAG	2 cut(s)	384, 1110
Eco91I	GGTNACC	1 cut(s)	409
EcoO65I	GGTNACC	1 cut(s)	409
EcoRII	CCWGG	1 cut(s)	619
EcoT38I	GRGCYC	1 cut(s)	862
FaeI	CATG	6 cut(s)	207, 299, 394, 797, 803, 821
FatI	CATG	6 cut(s)	203, 295, 390, 793, 799, 817
FbaI	TGATCA	1 cut(s)	292
FblI	GTMKAC	1 cut(s)	366
Fnu4HI	GCNGC	8 cut(s)	20, 23, 26, 312, 333, 525, 750, 1065
FokI	GGATG	8 cut(s)	114, 227, 429, 473, 524, 848, 1083, 1275
FriOI	GRGCYC	1 cut(s)	862
Fsp4HI	GCNGC	8 cut(s)	20, 23, 26, 312, 333, 525, 750, 1065
FspBI	CTAG	1 cut(s)	1294
GluI	GCNGC	8 cut(s)	20, 23, 26, 312, 333, 525, 750, 1065
GsaI	CCCAGC	2 cut(s)	82, 889
GsuI	CTGGAG	1 cut(s)	876
HaeIII	GGCC	3 cut(s)	5, 106, 669
HapII	CCGG	3 cut(s)	107, 212, 407
HgaI	GACGC	3 cut(s)	62, 187, 990
Hin1I	GRCGYC	2 cut(s)	198, 982
Hin1II	CATG	6 cut(s)	207, 299, 394, 797, 803, 821
HinfI	GANTC	4 cut(s)	112, 323, 359, 1086
HpaII	CCGG	3 cut(s)	107, 212, 407
HphI	GGTGA	2 cut(s)	1196, 1268
Hpy166II	GTNNAC	3 cut(s)	349, 367, 916
Hpy188I	TCNGA	5 cut(s)	164, 241, 403, 937, 1179
Hpy188III	TCNNGA	5 cut(s)	437, 471, 800, 1004, 1151
Hpy8I	GTNNAC	3 cut(s)	349, 367, 916
HpyAV	CCTTC	4 cut(s)	54, 151, 659, 797
HpyCH4III	ACNGT	4 cut(s)	1010, 1078, 1196, 1256
HpyCH4IV	ACGT	1 cut(s)	481
HpyCH4V	TGCA	9 cut(s)	335, 425, 486, 752, 793, 1018, 1033, 1174, 1247
HpyF10VI	GCNNNNNNNGC	2 cut(s)	25, 503
HpyF3I	CTNAG	1 cut(s)	1178
HpySE526I	ACGT	1 cut(s)	481
Hsp92I	GRCGYC	2 cut(s)	198, 982
Hsp92II	CATG	6 cut(s)	207, 299, 394, 797, 803, 821
KpnI	GGTACC	1 cut(s)	385
KroI	GCCGGC	1 cut(s)	106
KroNI	GCCGGC	1 cut(s)	108
Ksp22I	TGATCA	1 cut(s)	292
Kzo9I	GATC	4 cut(s)	292, 868, 1027, 1147
LguI	GCTCTTC	1 cut(s)	9
LmnI	GCTCC	1 cut(s)	857
Lsp1109I	GCAGC	6 cut(s)	34, 37, 298, 319, 511, 736
LweI	GCATC	4 cut(s)	451, 493, 1141, 1183
MaeI	CTAG	1 cut(s)	1294
MaeII	ACGT	1 cut(s)	481
MaeIII	GTNAC	3 cut(s)	199, 409, 437
MalI	GATC	4 cut(s)	294, 870, 1029, 1149
MbiI	CCGCTC	1 cut(s)	19
MboI	GATC	4 cut(s)	292, 868, 1027, 1147
MboII	GAAGA	9 cut(s)	20, 23, 26, 332, 459, 776, 950, 1116, 1149
MfeI	CAATTG	1 cut(s)	534
MflI	RGATCY	1 cut(s)	868
MhlI	GDGCHC	1 cut(s)	862
MluCI	AATT	6 cut(s)	37, 60, 174, 534, 841, 1169
MlyI	GAGTC	1 cut(s)	368
MmeI	TCCRAC	1 cut(s)	728
MnlI	CCTC	2 cut(s)	235, 346
MroNI	GCCGGC	1 cut(s)	106
MseI	TTAA	1 cut(s)	1080
MslI	CAYNNNNRTG	4 cut(s)	798, 804, 1023, 1179
MspI	CCGG	3 cut(s)	107, 212, 407
MspR9I	CCNGG	2 cut(s)	407, 621
MunI	CAATTG	1 cut(s)	534
Mva1269I	GAATGC	1 cut(s)	723
MvaI	CCWGG	1 cut(s)	621
MwoI	GCNNNNNNNGC	2 cut(s)	25, 503
NaeI	GCCGGC	1 cut(s)	108
NciI	CCSGG	1 cut(s)	407
NdeII	GATC	4 cut(s)	292, 868, 1027, 1147
NgoMIV	GCCGGC	1 cut(s)	106
NlaIII	CATG	6 cut(s)	207, 299, 394, 797, 803, 821
NlaIV	GGNNCC	4 cut(s)	383, 777, 859, 870
NmuCI	GTSAC	2 cut(s)	199, 437
NspI	RCATGY	2 cut(s)	394, 821
PagI	TCATGA	1 cut(s)	799
PciI	ACATGT	2 cut(s)	390, 817
PciSI	GCTCTTC	1 cut(s)	9
PctI	GAATGC	1 cut(s)	723
PdiI	GCCGGC	1 cut(s)	108
PfeI	GAWTC	3 cut(s)	112, 323, 1086
PflMI	CCANNNNNTGG	1 cut(s)	278
PkrI	GCNGC	8 cut(s)	21, 24, 27, 313, 334, 526, 751, 1066
PleI	GAGTC	1 cut(s)	367
PpsI	GAGTC	1 cut(s)	367
PscI	ACATGT	2 cut(s)	390, 817
PsiI	TTATAA	1 cut(s)	1266
Psp6I	CCWGG	1 cut(s)	619
PspEI	GGTNACC	1 cut(s)	409
PspFI	CCCAGC	2 cut(s)	78, 885
PspGI	CCWGG	1 cut(s)	619
PspN4I	GGNNCC	4 cut(s)	383, 777, 859, 870
PsuI	RGATCY	1 cut(s)	868
RsaI	GTAC	3 cut(s)	383, 917, 994
RsaNI	GTAC	3 cut(s)	382, 916, 993
RseI	CAYNNNNRTG	4 cut(s)	798, 804, 1023, 1179
SapI	GCTCTTC	1 cut(s)	9
SaqAI	TTAA	1 cut(s)	1080
SatI	GCNGC	8 cut(s)	20, 23, 26, 312, 333, 525, 750, 1065
Sau3AI	GATC	4 cut(s)	292, 868, 1027, 1147
SchI	GAGTC	1 cut(s)	368
ScrFI	CCNGG	2 cut(s)	407, 621
SduI	GDGCHC	1 cut(s)	862
SfaNI	GCATC	4 cut(s)	451, 493, 1141, 1183
SfcI	CTRYAG	2 cut(s)	306, 726
SmiMI	CAYNNNNRTG	4 cut(s)	798, 804, 1023, 1179
SmlI	CTYRAG	1 cut(s)	191
SmoI	CTYRAG	1 cut(s)	191
Sse9I	AATT	6 cut(s)	37, 60, 174, 534, 841, 1169
SsiI	CCGC	5 cut(s)	19, 131, 151, 687, 1064
SspI	AATATT	2 cut(s)	554, 641
SspMI	CTAG	1 cut(s)	1294
StyD4I	CCNGG	2 cut(s)	405, 619
TaaI	ACNGT	4 cut(s)	1010, 1078, 1196, 1256
TaiI	ACGT	1 cut(s)	484
TaqI	TCGA	3 cut(s)	120, 644, 1135
TasI	AATT	6 cut(s)	37, 60, 174, 534, 841, 1169
TatI	WGTACW	2 cut(s)	915, 992
TauI	GCSGC	2 cut(s)	22, 1067
TfiI	GAWTC	3 cut(s)	112, 323, 1086
Tru1I	TTAA	1 cut(s)	1080
Tru9I	TTAA	1 cut(s)	1080
TseFI	GTSAC	2 cut(s)	199, 437
TseI	GCWGC	6 cut(s)	22, 25, 311, 332, 524, 749
Tsp45I	GTSAC	2 cut(s)	199, 437
TspDTI	ATGAA	7 cut(s)	116, 333, 440, 528, 816, 1085, 1203
TspGWI	ACGGA	2 cut(s)	583, 967
Van91I	CCANNNNNTGG	1 cut(s)	278
XceI	RCATGY	2 cut(s)	394, 821
XmiI	GTMKAC	1 cut(s)	366
XspI	CTAG	1 cut(s)	1294

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.