AT4G21585

endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0007730
Physical Location & Seq
Forward (+)
11477814 .. 11480179
2366 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G21585.1

Sequence Viewer

Length: 900 bp
ATGAGTTCGTCGCTGCGACAGTGGTTTGCTAGAGTACTCGTGCTTACACAGCTCATCAATGGAGCTCTGTGTTGGGGAAAAGAAGGTCACTACACAGTCTGCAAAATAGCCGAGAGCTACTTTGAGGAAGAAACTGTAGCTGCGGTGAAGAAACTTCTACCTAAATCAGCTGATGGAGATCTAGCATCTGTTTGCTCATGGCCTGATGAGATTAAGCACCATTGGCAATGGCGATGGACTAGCCCTTTGCACTATGTTGACACACCTGATTACAGATGCAACTACGAGTATTGTCGGGATTGCCATGATACTCATAAGAATCAAGACCGGTGTGTGACAGGAGCTATTTTCAATTACACTATGCAACTTATGTCCGCTTCTGAAAACTCGGATACTATAGTCCACTACAACTTGACAGAGGCTCTCATGTTCTTATCACATTTTATTGGAGATATTCACCAGCCCTTGCATGTTGGTTTTCTGGGAGATGAAGGTGGAAACACGATAACAGTCCGCTGGTACCGTCGCAAAACAAATTTACACCATGTCTGGGATAACATGATAATTGAGTCTGCTCTTAAAACATACTACAATAAAAGTCTTCCACTCATGATTGAAGCACTTCAAGCCAATCTTACGAACGACTGGTCAAATGATGTTCCATTGTGGGAGTCATGTCAACTTAACCAAACGGCCTGTCCAAATCCGTACGCATCTGAAAGCATAAATCTAGCCTGCAAGTATGCTTATAGGAACGCTACCCCAGGGACTACTTTAGGAGATGACTATTTCCTCTCTCGGTTACCCATTGTGGAGAAGAGACTTGCGCAAGGTGGGATACGCTTGGCAGCCACTCTTAACCGTATCTTTTCTTCAAAACCTAAGCATGCTGGATCATGA

Protein Analysis

299

Amino Acids

34.2

Weight (kDa)

6.49

Isoelectric Point (pI)

42.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S1-P1_nuclease PF02265 25 - 290 3.4e-90 S1/P1 Nuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 828
Acc65I GGTACC 1 cut(s) 519
AccB1I GGYRCC 1 cut(s) 519
AciI CCGC 3 cut(s) 143, 375, 514
AcsI RAATTY 1 cut(s) 535
AfaI GTAC 3 cut(s) 36, 521, 710
AfiI CCNNNNNNNGG 1 cut(s) 550
AgeI ACCGGT 1 cut(s) 327
AgsI TTSAA 4 cut(s) 352, 617, 626, 876
AjnI CCWGG 1 cut(s) 763
AluBI AGCT 6 cut(s) 52, 65, 117, 140, 170, 344
AluI AGCT 6 cut(s) 52, 65, 117, 140, 170, 344
Alw21I GWGCWC 1 cut(s) 67
Alw26I GTCTC 1 cut(s) 814
AoxI GGCC 2 cut(s) 200, 693
ApeKI GCWGC 3 cut(s) 13, 140, 848
ApoI RAATTY 1 cut(s) 535
ArsI GACNNNNNNTTYG 6 cut(s) 229, 261, 632, 664, 682, 714
AsiGI ACCGGT 1 cut(s) 327
Asp700I GAANNNNTTC 1 cut(s) 621
Asp718I GGTACC 1 cut(s) 519
AspLEI GCGC 1 cut(s) 829
AsuHPI GGTGA 2 cut(s) 157, 449
BaeI ACNNNNGTAYC 2 cut(s) 384, 417
BanI GGYRCC 1 cut(s) 519
BanII GRGCYC 1 cut(s) 67
BarI GAAGNNNNNNTAC 2 cut(s) 856, 888
BauI CACGAG 1 cut(s) 38
BbsI GAAGAC 1 cut(s) 593
Bbv12I GWGCWC 1 cut(s) 67
BbvI GCAGC 2 cut(s) 127, 860
BccI CCATC 2 cut(s) 167, 228
BceAI ACGGC 1 cut(s) 708
BciT130I CCWGG 1 cut(s) 765
BciVI GTATCC 2 cut(s) 385, 831
BcoDI GTCTC 1 cut(s) 814
BfaI CTAG 4 cut(s) 30, 182, 240, 731
BfmI CTRYAG 2 cut(s) 135, 396
BfuI GTATCC 2 cut(s) 385, 831
BglII AGATCT 1 cut(s) 178
BisI GCNGC 3 cut(s) 14, 141, 849
BlsI GCNGC 3 cut(s) 15, 142, 850
BmcAI AGTACT 1 cut(s) 36
Bme1390I CCNGG 1 cut(s) 765
BmiI GGNNCC 1 cut(s) 521
BmrFI CCNGG 1 cut(s) 765
BmsI GCATC 3 cut(s) 194, 266, 722
BpiI GAAGAC 1 cut(s) 593
Bpu10I CCTNAGC 1 cut(s) 882
BsaBI GATNNNNATC 1 cut(s) 177
BsaJI CCNNGG 2 cut(s) 763, 764
BsaWI WCCGGW 1 cut(s) 327
Bsc4I CCNNNNNNNGG 1 cut(s) 550
Bse118I RCCGGY 1 cut(s) 327
Bse1I ACTGG 1 cut(s) 650
Bse3DI GCAATG 1 cut(s) 233
Bse8I GATNNNNATC 1 cut(s) 177
BseBI CCWGG 1 cut(s) 765
BseDI CCNNGG 2 cut(s) 763, 764
BseJI GATNNNNATC 1 cut(s) 177
BseLI CCNNNNNNNGG 1 cut(s) 550
BseMI GCAATG 1 cut(s) 233
BseNI ACTGG 1 cut(s) 650
BseXI GCAGC 2 cut(s) 127, 860
BshFI GGCC 2 cut(s) 202, 695
BshNI GGYRCC 1 cut(s) 519
BshTI ACCGGT 1 cut(s) 327
BsiHKAI GWGCWC 1 cut(s) 67
BsiSI CCGG 1 cut(s) 328
BsiWI CGTACG 1 cut(s) 708
BslFI GGGAC 1 cut(s) 781
BslI CCNNNNNNNGG 1 cut(s) 550
BsmAI GTCTC 1 cut(s) 814
BsmFI GGGAC 1 cut(s) 781
BsnI GGCC 2 cut(s) 202, 695
Bsp1286I GDGCHC 1 cut(s) 67
Bsp143I GATC 2 cut(s) 178, 893
BspACI CCGC 3 cut(s) 143, 375, 514
BspANI GGCC 2 cut(s) 202, 695
BspHI TCATGA 2 cut(s) 609, 896
BspLI GGNNCC 1 cut(s) 521
BspT107I GGYRCC 1 cut(s) 519
BsrDI GCAATG 1 cut(s) 233
BsrFI RCCGGY 1 cut(s) 327
BsrI ACTGG 1 cut(s) 650
BssAI RCCGGY 1 cut(s) 327
BssECI CCNNGG 2 cut(s) 763, 764
BssMI GATC 2 cut(s) 178, 893
BssSI CACGAG 1 cut(s) 38
Bst2BI CACGAG 1 cut(s) 38
Bst2UI CCWGG 1 cut(s) 765
Bst4CI ACNGT 6 cut(s) 21, 97, 136, 511, 524, 863
Bst6I CTCTTC 1 cut(s) 812
BstC8I GCNNGC 2 cut(s) 736, 888
BstDEI CTNAG 1 cut(s) 882
BstEII GGTNACC 1 cut(s) 801
BstHHI GCGC 1 cut(s) 829
BstKTI GATC 2 cut(s) 181, 896
BstMAI GTCTC 1 cut(s) 814
BstMBI GATC 2 cut(s) 178, 893
BstMWI GCNNNNNNNGC 3 cut(s) 49, 223, 626
BstNI CCWGG 1 cut(s) 765
BstNSI RCATGY 2 cut(s) 473, 890
BstPI GGTNACC 1 cut(s) 801
BstSCI CCNGG 1 cut(s) 763
BstSFI CTRYAG 2 cut(s) 135, 396
BstV1I GCAGC 2 cut(s) 127, 860
BstV2I GAAGAC 1 cut(s) 593
BstX2I RGATCY 1 cut(s) 178
BstYI RGATCY 1 cut(s) 178
BsuI GTATCC 2 cut(s) 385, 831
BsuRI GGCC 2 cut(s) 202, 695
BtgZI GCGATG 1 cut(s) 247
BtsIMutI CAGTG 1 cut(s) 26
Cac8I GCNNGC 2 cut(s) 736, 888
CciI TCATGA 2 cut(s) 609, 896
CfoI GCGC 1 cut(s) 829
Cfr10I RCCGGY 1 cut(s) 327
Csp6I GTAC 3 cut(s) 35, 520, 709
CspAI ACCGGT 1 cut(s) 327
CviQI GTAC 3 cut(s) 35, 520, 709
DdeI CTNAG 1 cut(s) 882
DpnI GATC 2 cut(s) 180, 895
DpnII GATC 2 cut(s) 178, 893
Eam1104I CTCTTC 1 cut(s) 812
EarI CTCTTC 1 cut(s) 812
Ecl136II GAGCTC 1 cut(s) 65
Eco24I GRGCYC 1 cut(s) 67
Eco53kI GAGCTC 1 cut(s) 65
Eco91I GGTNACC 1 cut(s) 801
EcoICRI GAGCTC 1 cut(s) 65
EcoO65I GGTNACC 1 cut(s) 801
EcoRII CCWGG 1 cut(s) 763
EcoT38I GRGCYC 1 cut(s) 67
FalI AAGNNNNNCTT 2 cut(s) 618, 650
FaqI GGGAC 1 cut(s) 781
Fnu4HI GCNGC 3 cut(s) 14, 141, 849
FriOI GRGCYC 1 cut(s) 67
Fsp4HI GCNGC 3 cut(s) 14, 141, 849
FspBI CTAG 4 cut(s) 30, 182, 240, 731
FspI TGCGCA 1 cut(s) 828
GlaI GCGC 1 cut(s) 828
GluI GCNGC 3 cut(s) 14, 141, 849
HaeIII GGCC 2 cut(s) 202, 695
HapII CCGG 1 cut(s) 328
HhaI GCGC 1 cut(s) 829
Hin6I GCGC 1 cut(s) 827
HinP1I GCGC 1 cut(s) 827
HincII GTYRAC 2 cut(s) 259, 680
HindII GTYRAC 2 cut(s) 259, 680
HinfI GANTC 3 cut(s) 319, 569, 671
HpaII CCGG 1 cut(s) 328
HphI GGTGA 2 cut(s) 157, 449
Hpy166II GTNNAC 3 cut(s) 259, 403, 680
Hpy188I TCNGA 3 cut(s) 382, 391, 718
Hpy188III TCNNGA 4 cut(s) 296, 323, 610, 897
Hpy8I GTNNAC 3 cut(s) 259, 403, 680
Hpy99I CGWCG 2 cut(s) 13, 528
HpyAV CCTTC 2 cut(s) 77, 485
HpyCH4III ACNGT 6 cut(s) 21, 97, 136, 511, 524, 863
HpyCH4V TGCA 6 cut(s) 102, 250, 279, 364, 469, 738
HpyF10VI GCNNNNNNNGC 3 cut(s) 49, 223, 626
HpyF3I CTNAG 1 cut(s) 882
HspAI GCGC 1 cut(s) 827
KpnI GGTACC 1 cut(s) 523
Kzo9I GATC 2 cut(s) 178, 893
LmnI GCTCC 2 cut(s) 62, 341
Lsp1109I GCAGC 2 cut(s) 127, 860
LweI GCATC 3 cut(s) 194, 266, 722
MaeI CTAG 4 cut(s) 30, 182, 240, 731
MaeIII GTNAC 3 cut(s) 86, 334, 801
MalI GATC 2 cut(s) 180, 895
MboI GATC 2 cut(s) 178, 893
MboII GAAGA 5 cut(s) 140, 160, 593, 829, 864
MflI RGATCY 1 cut(s) 178
MhlI GDGCHC 1 cut(s) 67
MluCI AATT 3 cut(s) 352, 535, 564
MlyI GAGTC 2 cut(s) 578, 680
MnlI CCTC 3 cut(s) 118, 412, 803
MroXI GAANNNNTTC 1 cut(s) 621
MseI TTAA 4 cut(s) 213, 579, 684, 858
MspA1I CMGCKG 2 cut(s) 170, 516
MspI CCGG 1 cut(s) 328
MspR9I CCNGG 1 cut(s) 765
MvaI CCWGG 1 cut(s) 765
MwoI GCNNNNNNNGC 3 cut(s) 49, 223, 626
NdeII GATC 2 cut(s) 178, 893
NlaIV GGNNCC 1 cut(s) 521
NmeAIII GCCGAG 1 cut(s) 136
NmuCI GTSAC 2 cut(s) 86, 334
NsbI TGCGCA 1 cut(s) 828
NspI RCATGY 2 cut(s) 473, 890
PaeI GCATGC 1 cut(s) 890
PagI TCATGA 2 cut(s) 609, 896
PasI CCCWGGG 1 cut(s) 764
PdmI GAANNNNTTC 1 cut(s) 621
PfeI GAWTC 1 cut(s) 319
Pfl23II CGTACG 1 cut(s) 708
PinAI ACCGGT 1 cut(s) 327
PkrI GCNGC 3 cut(s) 15, 142, 850
PleI GAGTC 2 cut(s) 577, 679
PpsI GAGTC 2 cut(s) 577, 679
Psp124BI GAGCTC 1 cut(s) 67
Psp6I CCWGG 1 cut(s) 763
PspEI GGTNACC 1 cut(s) 801
PspGI CCWGG 1 cut(s) 763
PspLI CGTACG 1 cut(s) 708
PspN4I GGNNCC 1 cut(s) 521
PsuI RGATCY 1 cut(s) 178
PvuII CAGCTG 1 cut(s) 170
RsaI GTAC 3 cut(s) 36, 521, 710
RsaNI GTAC 3 cut(s) 35, 520, 709
SacI GAGCTC 1 cut(s) 67
SaqAI TTAA 4 cut(s) 213, 579, 684, 858
SatI GCNGC 3 cut(s) 14, 141, 849
Sau3AI GATC 2 cut(s) 178, 893
ScaI AGTACT 1 cut(s) 36
SchI GAGTC 2 cut(s) 578, 680
ScrFI CCNGG 1 cut(s) 765
SduI GDGCHC 1 cut(s) 67
SfaNI GCATC 3 cut(s) 194, 266, 722
SfcI CTRYAG 2 cut(s) 135, 396
SphI GCATGC 1 cut(s) 890
Sse9I AATT 3 cut(s) 352, 535, 564
SsiI CCGC 3 cut(s) 143, 375, 514
SspMI CTAG 4 cut(s) 30, 182, 240, 731
SstI GAGCTC 1 cut(s) 67
StyD4I CCNGG 1 cut(s) 763
TaaI ACNGT 6 cut(s) 21, 97, 136, 511, 524, 863
TasI AATT 3 cut(s) 352, 535, 564
TatI WGTACW 1 cut(s) 34
TfiI GAWTC 1 cut(s) 319
Tru1I TTAA 4 cut(s) 213, 579, 684, 858
Tru9I TTAA 4 cut(s) 213, 579, 684, 858
TscAI CASTG 1 cut(s) 26
TseFI GTSAC 2 cut(s) 86, 334
TseI GCWGC 3 cut(s) 13, 140, 848
Tsp45I GTSAC 2 cut(s) 86, 334
TspDTI ATGAA 1 cut(s) 504
TspGWI ACGGA 1 cut(s) 696
TspRI CASTG 1 cut(s) 26
XapI RAATTY 1 cut(s) 535
XceI RCATGY 2 cut(s) 473, 890
XmnI GAANNNNTTC 1 cut(s) 621
XspI CTAG 4 cut(s) 30, 182, 240, 731
ZrmI AGTACT 1 cut(s) 36
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.