AT4G21600

endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0007730
Physical Location & Seq
Forward (+)
11484981 .. 11487535
2555 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G21600.1

Sequence Viewer

Length: 891 bp
ATGAGATTGTGGATTGTGAGTGTACTCGTGCTTACACACCTTGTCCATGGAGCTCTCTGTTGGGGGAAGGATGGTCACTACACTGTTTGCAAATTAGCTGAGGGATTCTTTGAGGATGATACTATTGCTGCAGTGAAGAAACTTCTGCCTGAATCAGTTGATGGTGGTGGACTAGCAGATTTTTGCTCATGGCCTGATGAAATTAAAAAACTCTCGCAATGGCAATGGACTAGCACTTTGCACTATGTTAACACACCTGAATACAGGTGCAACTACGAGTATTGCCGGGACTGCCATGACACTCATAAGCATAAGGACTGGTGTGTGACAGGAGCGATCTTCAATTACACAAACCAGTTAATGTCTGCTTCTGAAAACTCACAGAATATAGTCCACTACAACTTGACAGAGGCTCTCTTGTTCTTGTCACATTATATGGGAGATGTTCATCAGCCCTTGCATACTGGTTTTCTTGGAGATCTAGGTGGTAACACAATAATAGTCAACTGGTACCATAACAAATCAAATTTGCACCATGTCTGGGACAACATGATAATTGACTCTGCTCTAGAAACATACTACAATTCAAGCCTTCCACACATGATTCAAGCTCTTCAAGCCAAACTCAAGAACGGCTGGTCGAATGATGTTCCGTCGTGGAAGTCATGTCACTTTCACCAAAAGGCCTGTCCAAATCTGTATGCTTCTGAAAGCATTGATCTCGCCTGCAAGTATGCTTACAGGAACGCTACTCCGGGGACTACTTTAGGAGATGAGTATTTCCTTTCTCGGTTACCCGTTGTAGAGAAGAGACTTGCACAAGGTGGGATTCGCTTGGCAGCTACTCTTAATCGTATCTTTTCTGCAAAACCGAAGCTCGCAGGATTATGA

Protein Analysis

296

Amino Acids

33.61

Weight (kDa)

6.61

Isoelectric Point (pI)

38.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S1-P1_nuclease PF02265 21 - 287 1.7e-84 S1/P1 Nuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 510
AccB1I GGYRCC 1 cut(s) 510
AcsI RAATTY 1 cut(s) 526
AdeI CACNNNGTG 1 cut(s) 824
AfaI GTAC 2 cut(s) 24, 512
AfiI CCNNNNNNNGG 1 cut(s) 541
AgsI TTSAA 4 cut(s) 343, 588, 608, 617
AluBI AGCT 5 cut(s) 53, 98, 611, 842, 877
AluI AGCT 5 cut(s) 53, 98, 611, 842, 877
Alw21I GWGCWC 1 cut(s) 55
Alw26I GTCTC 1 cut(s) 805
AoxI GGCC 2 cut(s) 191, 684
ApeKI GCWGC 2 cut(s) 128, 839
ApoI RAATTY 1 cut(s) 526
ArsI GACNNNNNNTTYG 4 cut(s) 220, 252, 673, 705
Asp718I GGTACC 1 cut(s) 510
AsuC2I CCSGG 2 cut(s) 287, 756
AsuHPI GGTGA 1 cut(s) 668
BanI GGYRCC 1 cut(s) 510
BanII GRGCYC 1 cut(s) 55
BauI CACGAG 1 cut(s) 26
Bbv12I GWGCWC 1 cut(s) 55
BbvCI CCTCAGC 1 cut(s) 99
BbvI GCAGC 2 cut(s) 115, 851
BccI CCATC 2 cut(s) 65, 155
BceAI ACGGC 1 cut(s) 649
BcgI CGANNNNNNTGC 2 cut(s) 703, 737
BcnI CCSGG 2 cut(s) 287, 756
BcoDI GTCTC 1 cut(s) 805
BfaI CTAG 4 cut(s) 173, 231, 482, 569
BfmI CTRYAG 1 cut(s) 129
BglII AGATCT 1 cut(s) 478
BisI GCNGC 2 cut(s) 129, 840
BlsI GCNGC 2 cut(s) 130, 841
Bme1390I CCNGG 2 cut(s) 287, 756
BmiI GGNNCC 1 cut(s) 512
BmrFI CCNGG 2 cut(s) 287, 756
Bpu10I CCTNAGC 1 cut(s) 99
BpuEI CTTGAG 1 cut(s) 611
BpuMI CCSGG 2 cut(s) 287, 756
BsaBI GATNNNNATC 1 cut(s) 447
BsaJI CCNNGG 2 cut(s) 46, 755
Bsc4I CCNNNNNNNGG 1 cut(s) 541
Bse1I ACTGG 4 cut(s) 323, 355, 469, 512
Bse3DI GCAATG 2 cut(s) 224, 230
Bse8I GATNNNNATC 1 cut(s) 447
BseDI CCNNGG 2 cut(s) 46, 755
BseGI GGATG 2 cut(s) 76, 121
BseJI GATNNNNATC 1 cut(s) 447
BseLI CCNNNNNNNGG 1 cut(s) 541
BseMI GCAATG 2 cut(s) 224, 230
BseMII CTCAG 1 cut(s) 90
BseNI ACTGG 4 cut(s) 323, 355, 469, 512
BseXI GCAGC 2 cut(s) 115, 851
BshFI GGCC 2 cut(s) 193, 686
BshNI GGYRCC 1 cut(s) 510
BsiHKAI GWGCWC 1 cut(s) 55
BsiSI CCGG 2 cut(s) 286, 755
BslFI GGGAC 3 cut(s) 302, 557, 772
BslI CCNNNNNNNGG 1 cut(s) 541
BsmAI GTCTC 1 cut(s) 805
BsmFI GGGAC 3 cut(s) 302, 557, 772
BsnI GGCC 2 cut(s) 193, 686
Bsp1286I GDGCHC 1 cut(s) 55
Bsp143I GATC 3 cut(s) 336, 478, 718
Bsp19I CCATGG 1 cut(s) 46
BspANI GGCC 2 cut(s) 193, 686
BspCNI CTCAG 1 cut(s) 91
BspLI GGNNCC 1 cut(s) 512
BspMAI CTGCAG 1 cut(s) 133
BspQI GCTCTTC 1 cut(s) 618
BspT107I GGYRCC 1 cut(s) 510
BsrDI GCAATG 2 cut(s) 224, 230
BsrI ACTGG 4 cut(s) 323, 355, 469, 512
BssECI CCNNGG 2 cut(s) 46, 755
BssMI GATC 3 cut(s) 336, 478, 718
BssSI CACGAG 1 cut(s) 26
BssT1I CCWWGG 1 cut(s) 46
Bst2BI CACGAG 1 cut(s) 26
Bst4CI ACNGT 1 cut(s) 85
Bst6I CTCTTC 2 cut(s) 618, 803
BstC8I GCNNGC 2 cut(s) 727, 879
BstDEI CTNAG 1 cut(s) 99
BstDSI CCRYGG 1 cut(s) 46
BstEII GGTNACC 1 cut(s) 792
BstF5I GGATG 2 cut(s) 76, 121
BstKTI GATC 3 cut(s) 339, 481, 721
BstMAI GTCTC 1 cut(s) 805
BstMBI GATC 3 cut(s) 336, 478, 718
BstMWI GCNNNNNNNGC 2 cut(s) 291, 617
BstPI GGTNACC 1 cut(s) 792
BstSCI CCNGG 2 cut(s) 285, 754
BstSFI CTRYAG 1 cut(s) 129
BstV1I GCAGC 2 cut(s) 115, 851
BstX2I RGATCY 1 cut(s) 478
BstYI RGATCY 1 cut(s) 478
BsuRI GGCC 2 cut(s) 193, 686
BtgI CCRYGG 1 cut(s) 46
BtsCI GGATG 2 cut(s) 76, 121
BtsI GCAGTG 1 cut(s) 138
BtsIMutI CAGTG 2 cut(s) 81, 138
Cac8I GCNNGC 2 cut(s) 727, 879
Csp6I GTAC 2 cut(s) 23, 511
CviAII CATG 7 cut(s) 47, 189, 296, 536, 550, 601, 666
CviQI GTAC 2 cut(s) 23, 511
DdeI CTNAG 1 cut(s) 99
DpnI GATC 3 cut(s) 338, 480, 720
DpnII GATC 3 cut(s) 336, 478, 718
DraIII CACNNNGTG 1 cut(s) 824
Eam1104I CTCTTC 2 cut(s) 618, 803
EarI CTCTTC 2 cut(s) 618, 803
Ecl136II GAGCTC 1 cut(s) 53
Eco130I CCWWGG 1 cut(s) 46
Eco147I AGGCCT 1 cut(s) 686
Eco24I GRGCYC 1 cut(s) 55
Eco53kI GAGCTC 1 cut(s) 53
Eco91I GGTNACC 1 cut(s) 792
EcoICRI GAGCTC 1 cut(s) 53
EcoO65I GGTNACC 1 cut(s) 792
EcoT14I CCWWGG 1 cut(s) 46
EcoT38I GRGCYC 1 cut(s) 55
ErhI CCWWGG 1 cut(s) 46
FaeI CATG 7 cut(s) 50, 192, 299, 539, 553, 604, 669
FaqI GGGAC 3 cut(s) 302, 557, 772
FatI CATG 7 cut(s) 46, 188, 295, 535, 549, 600, 665
Fnu4HI GCNGC 2 cut(s) 129, 840
FokI GGATG 2 cut(s) 83, 128
FriOI GRGCYC 1 cut(s) 55
Fsp4HI GCNGC 2 cut(s) 129, 840
FspBI CTAG 4 cut(s) 173, 231, 482, 569
GluI GCNGC 2 cut(s) 129, 840
HaeIII GGCC 2 cut(s) 193, 686
HapII CCGG 2 cut(s) 286, 755
Hin1II CATG 7 cut(s) 50, 192, 299, 539, 553, 604, 669
HincII GTYRAC 2 cut(s) 250, 505
HindII GTYRAC 2 cut(s) 250, 505
HinfI GANTC 5 cut(s) 105, 152, 560, 604, 829
HpaI GTTAAC 1 cut(s) 250
HpaII CCGG 2 cut(s) 286, 755
HphI GGTGA 1 cut(s) 668
Hpy166II GTNNAC 5 cut(s) 23, 170, 250, 394, 505
Hpy188I TCNGA 2 cut(s) 373, 709
Hpy188III TCNNGA 2 cut(s) 569, 628
Hpy8I GTNNAC 5 cut(s) 23, 170, 250, 394, 505
Hpy99I CGWCG 1 cut(s) 658
HpyAV CCTTC 2 cut(s) 61, 602
HpyCH4III ACNGT 1 cut(s) 85
HpyCH4V TGCA 9 cut(s) 90, 131, 241, 270, 460, 532, 729, 818, 866
HpyF10VI GCNNNNNNNGC 2 cut(s) 291, 617
HpyF3I CTNAG 1 cut(s) 99
Hsp92II CATG 7 cut(s) 50, 192, 299, 539, 553, 604, 669
KpnI GGTACC 1 cut(s) 514
KspAI GTTAAC 1 cut(s) 250
Kzo9I GATC 3 cut(s) 336, 478, 718
LguI GCTCTTC 1 cut(s) 618
LmnI GCTCC 2 cut(s) 50, 332
Lsp1109I GCAGC 2 cut(s) 115, 851
MaeI CTAG 4 cut(s) 173, 231, 482, 569
MaeIII GTNAC 6 cut(s) 74, 325, 426, 488, 668, 792
MalI GATC 3 cut(s) 338, 480, 720
MboI GATC 3 cut(s) 336, 478, 718
MboII GAAGA 4 cut(s) 148, 331, 605, 820
MflI RGATCY 1 cut(s) 478
MhlI GDGCHC 1 cut(s) 55
MluCI AATT 6 cut(s) 92, 201, 343, 526, 555, 583
MlyI GAGTC 1 cut(s) 554
MnlI CCTC 3 cut(s) 94, 106, 403
MseI TTAA 4 cut(s) 204, 249, 359, 849
MspI CCGG 2 cut(s) 286, 755
MspR9I CCNGG 2 cut(s) 287, 756
MwoI GCNNNNNNNGC 2 cut(s) 291, 617
NciI CCSGG 2 cut(s) 287, 756
NcoI CCATGG 1 cut(s) 46
NdeII GATC 3 cut(s) 336, 478, 718
NlaIII CATG 7 cut(s) 50, 192, 299, 539, 553, 604, 669
NlaIV GGNNCC 1 cut(s) 512
NmuCI GTSAC 4 cut(s) 74, 325, 426, 668
PceI AGGCCT 1 cut(s) 686
PciSI GCTCTTC 1 cut(s) 618
PfeI GAWTC 4 cut(s) 105, 152, 604, 829
PkrI GCNGC 2 cut(s) 130, 841
PleI GAGTC 1 cut(s) 554
PpsI GAGTC 1 cut(s) 554
Psp124BI GAGCTC 1 cut(s) 55
PspEI GGTNACC 1 cut(s) 792
PspN4I GGNNCC 1 cut(s) 512
PstI CTGCAG 1 cut(s) 133
PsuI RGATCY 1 cut(s) 478
RsaI GTAC 2 cut(s) 24, 512
RsaNI GTAC 2 cut(s) 23, 511
SacI GAGCTC 1 cut(s) 55
SapI GCTCTTC 1 cut(s) 618
SaqAI TTAA 4 cut(s) 204, 249, 359, 849
SatI GCNGC 2 cut(s) 129, 840
Sau3AI GATC 3 cut(s) 336, 478, 718
SchI GAGTC 1 cut(s) 554
ScrFI CCNGG 2 cut(s) 287, 756
SduI GDGCHC 1 cut(s) 55
SfcI CTRYAG 1 cut(s) 129
SmlI CTYRAG 1 cut(s) 626
SmoI CTYRAG 1 cut(s) 626
Sse9I AATT 6 cut(s) 92, 201, 343, 526, 555, 583
SseBI AGGCCT 1 cut(s) 686
SspMI CTAG 4 cut(s) 173, 231, 482, 569
SstI GAGCTC 1 cut(s) 55
StuI AGGCCT 1 cut(s) 686
StyD4I CCNGG 2 cut(s) 285, 754
StyI CCWWGG 1 cut(s) 46
TaaI ACNGT 1 cut(s) 85
TaqI TCGA 1 cut(s) 641
TasI AATT 6 cut(s) 92, 201, 343, 526, 555, 583
TatI WGTACW 1 cut(s) 22
TfiI GAWTC 4 cut(s) 105, 152, 604, 829
Tru1I TTAA 4 cut(s) 204, 249, 359, 849
Tru9I TTAA 4 cut(s) 204, 249, 359, 849
TscAI CASTG 2 cut(s) 88, 138
TseFI GTSAC 4 cut(s) 74, 325, 426, 668
TseI GCWGC 2 cut(s) 128, 839
Tsp45I GTSAC 4 cut(s) 74, 325, 426, 668
TspDTI ATGAA 2 cut(s) 213, 437
TspGWI ACGGA 1 cut(s) 642
TspRI CASTG 2 cut(s) 88, 138
XapI RAATTY 1 cut(s) 526
XbaI TCTAGA 1 cut(s) 568
XspI CTAG 4 cut(s) 173, 231, 482, 569
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.